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*  Coupling IC to HRAM-MS for the Fast and Robust Analysis of Anionic Pesticides in Fruits and Vegetables | Thermo Fisher...
Learn about the application and evaluation of ion chromatography coupled to Orbitrap mass spectrometry for multiresidue ... detection of polar pesticides in fruits and vegetables in this free, on-demand webinar. ... Additionally, their retention in the reversed-phase liquid chromatography columns typically used for pesticide analysis is ... Highly polar pesticides (glyphosate, AMPA, fosetyl-aluminum, etc.) are particularly challenging analytes to study. They have ...
  http://www.thermofisher.com/ca/en/home/global/forms/industrial/coupling-ic-hram-ms-analysis-pesticides-fruits-vegetables.html
*  Reversed-Phase HPLC Buffers | Sigma-Aldrich
... of the compound enhances retention. Dramatic effects are observed on retention of these analytes in pH range near the pKa of a ... Preparation of aqueous mobile phase is the most critical step in reversed-phase chromatography (RPC) method development for ... can result in poor or irreproducible retention and tailing in reversedphase separation of polar and ionizable compounds. ... Mobile Phase pH and Reversed-Phase Retention. Retention of ionic analytes in RPC is fundamentally affected by mobile phase pH. ...
  https://www.sigmaaldrich.com/technical-documents/articles/reporter-us/reversed-phase-hplc.html
*  Hypercarb HPLC and UHPLC Columns | Thermo Fisher Scientific - US
... has unique properties as a stationary phase in high performance liquid chromatography (HPLC). ... Your solution for difficult separations in both reversed phase and normal phase HPLC and LC/MS applications ... PGC behaves as a strongly retentive alkyl-bonded silica gel for non-polar analytes. However, its retention and selectivity ... Porous graphitic carbon (PGC, Hypercarb™) has unique properties as a stationary phase in high-performance liquid chromatography ...
  https://www.thermofisher.com/us/en/home/industrial/chromatography/liquid-chromatography-lc/hplc-uhplc-columns/hypercarb-hplc-uhplc-columns.html
*  Reversed-Phase SPE Methodology in Solid Phase Extraction | China-Mainland | Sigma-Aldrich
Reversed-phase SPE is considered the least selective retention mechanism when compared to normalphase or ion-exchange SPE. ... Reversed-Phase SPE Methodology in Solid Phase Extraction. ... Increase bed weight to retain more polar compounds.. *Concern ... Analyte Characteristics:. Analytes exhibiting non-polar functionalities *Most organic analytes *Alkyl, aromatic, alicyclic ... Analytical Chromatography Home * Analytical / Chromatography Products * Air Monitoring * Analytical Reagents & Solvents * ...
  https://www.sigmaaldrich.com/china-mainland/zh/analytical-chromatography/sample-preparation/spe/reversedphase-methodology.html
*  HPLC Application for Separation of Nucleotide Bases | SIELC
Compounds are retained by weak reverse phase and weak ion-exchange mechanisms. Method can utilize UV, ELSD, and LC/MS detection ... In the view of chromatography these compounds are very polar and similar in properties. It is hard to obtain base line ...
  http://www.sielc.com/Application-HPLC-Application-For-Separation-of-Nucleotide-Bases.html
*  HPLC Application for Retention of Guanidine by Mixed-Mode Chromatography | SIELC
Guanidine is highly basic (pKa 12.5), and a polar compound is formed by the oxidation of guanine. In the human body, guanine is ... If substituted guanidine has hydrophobic properties, it will retain based on ion-exchange and reverse phase mechanisms. ... HPLC Application for Retention of Guanidine by Mixed-Mode Chromatography. *Home. *HPLC Application for Retention of Guanidine ... This method can be used for analysis of other guanidine-based compounds. ...
  http://www.sielc.com/Application-HPLC-Application-For-Retention-of-Guanidine-By-Mixed-Mode-Chromatography.html
*  Reversed-phase chromatography - Wikipedia
"Reverse-phase chromatography of polar biological substances: separation of catechol compounds by high-performance liquid ... while cyano columns can be used in a reversed-phase mode depending on analyte and mobile phase conditions. Not all C18 columns ... hence the term reversed-phase chromatography. Reversed-phase chromatography employs a polar (aqueous) mobile phase. As a result ... Reversed-phase chromatography (also called RPC, reverse-phase chromatography, or hydrophobic chromatography) includes any ...
  https://en.wikipedia.org/wiki/Reversed-phase_chromatography
*  Water Analyses equipment (Water and Wastewater) | Water and Wastewater - Environmental XPRT
... for all classes of polar hydrophilic compounds. What is Hydrophilic Interaction Liquid Chromatography (HILIC)? One can say that ... HILIC is a normal-phase (NPLC) type of separation but uses reversed-phase (RPLC) type eluents. In HILIC one uses: - A column ... Model 33623 - Alkylphenol Target Analyte Mix Solution for DIN EN ISO 18857-2. Alkylphenol Target Analyte Mix solution for DIN ... L × I.D. 15 m × 0.10 mm, df 0.10 μmApplication: The 5% phenyl equivalent phase provides a boiling point elution order with a ...
  https://www.environmental-expert.com/water-wastewater/water-analyses/products
*  Quality and Efficacy of Tribulus terrestris as an Ingredient for Dermatological Formulations - PDF
In the widely used reversed phase methods polar compounds ... YMC HILIC Columns for Polar Analytes Polar Analytes Advantages ... italica) BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY (LC-MS) AND DETERMINATION OF ANTICANCER PROPERTIES OF BROCCOLI EXTRACTS ... YMC HILIC Columns for Polar Analytes YMC HILIC Columns for Polar Analytes Polar Analytes Advantages In general, reliable ... In the widely used reversed phase methods polar compounds ... Solid Phase Peptide Synthesis (SPPS) and Solid Phase. Fragment ...
  http://healthdocbox.com/Herbs_For_Health/71670678-Quality-and-efficacy-of-tribulus-terrestris-as-an-ingredient-for-dermatological-formulations.html
*  HPLC Columns | Lab Manager
... reverse-phase and normal-phase chromatography separation methods are based on polarity. Reverse phase columns separate analytes ... reverse-phase and normal-phase chromatography separation methods are based on polarity. Reverse phase columns separate analytes ... 6. Do you need a unique selectivity (HILIC, polar-end capped, etc.) to separate any very polar and/ or non-polar components in ... based on their hydrophobicity, with the more hydrophobic compounds being retained longer on the column. Separations based on ...
  http://www.labmanager.com/lab-product/2012/08/hplc-columns
*  Biotage - Search Literature
Reversed-phase chromatography is typically used when you need to separate several milligrams of relatively polar compounds that ... Analyte Type Acid herbicides. Acidic. Acidic catecholamine metabolites. Alcohol metabolite. Aliphatic hydrocarbons. Alkaloids. ... If you are currently using reversed-phase at preparative scale, such as flash chromatography, you know the mobile phase ... Greener reversed-phase Flash Chromatography Using Acetone Instead of Acetonitrile Part No: P158 Issued year: 2017 File size: ...
  http://www.biotage.com/literature/search/15/50?search_query=
*  Biotage - Search Literature
Reversed-phase chromatography is typically used when you need to separate several milligrams of relatively polar compounds that ... ENV+ is a hyper-cross linked polystyrene polymer sorbent capable of extracting a wide range of polar and water soluble analytes ... If you are currently using reversed-phase at preparative scale, such as flash chromatography, you know the mobile phase ... Impact of Injection Solvent Choice on Peak Shape and Resolution in Reversed-phase Flash Chromatography Part No: P140 Issued ...
  http://www.biotage.com/literature/search/8/100?search_query=
*  KNAUER LC Columns for HPLC, UHPLC, FPLC and preparative HPLC | KNAUER - Science Together
... liquid chromatography columns from KNAUER have been well-known for their high quality and performance. Choose from more than ... aqueous mobile phase. C18 stationary phase for the analysis of more polar and hydrophilic compounds in reversed phase mode (USP ... Columns for more unpolar analytes in Reversed Phase. The C8 stationary phase in Reversed Phase mode has alternative selectivity ... Si columns for Normal Phase and HILIC applications. Unmodified Silica gel materials were the first ones used for chromatography ...
  https://www.knauer.net/en/Systems-Solutions/LC-Columns
*  Micellar liquid chromatography - Wikipedia
Reverse phase high-performance liquid chromatography (RP-HPLC) involves a non-polar stationary phase, often a hydrocarbon chain ... and somewhat on the polar groups present in the stationary phase. 23 compounds were analyzed with varying mobile phases and ... When a solution of analytes is injected into the system, the components begin to partition out of the mobile phase and interact ... Micellar liquid chromatography (MLC) is a form of reversed phase liquid chromatography that uses an aqueous micellar solutions ...
  https://en.wikipedia.org/wiki/Micellar_liquid_chromatography
*  VANQUISH UHPLC Columns | Thermo Fisher Scientific - AR
Get a new level of liquid chromatography separation, speed and throughput. Vanquish UHPLC columns are designed for extended ... Use these reversed-phase VANQUISH columns for even greater power of separation, speed, and throughput of polar and nonpolar ... Use these columns for better, faster, and easier separation of polar analytes in high throughput environments. ... and easier separation of halogenated and non-halogenated polar compounds in complex mixtures. ...
  http://www.thermofisher.com/ar/es/home/industrial/chromatography/liquid-chromatography-lc/hplc-uhplc-columns/vanquish-uhplc-columns.html
*  Chromatography - Wikipedia
... is polar (e.g. paper, silica etc.) it is forward phase chromatography, and if it is non-polar (C-18) it is reverse phase. ... Gas chromatography is based on a partition equilibrium of analyte between a solid or viscous liquid stationary phase (often a ... Ion exchange chromatography uses a charged stationary phase to separate charged compounds including anions, cations, amino ... Reversed-phase chromatography[edit]. Main article: Reversed-phase chromatography. Reversed-phase chromatography (RPC) is any ...
  https://en.wikipedia.org/wiki/Chromatography
*  Hydrophilic Interaction Chromatography for the Characterization of Therapeutic Monoclonal Antibodies at Protein, Peptide, and...
A good example is hydrophilic interaction chromatography (HILIC). While the technique has been used for more than 20 years to ... A good example is hydrophilic interaction chromatography (HILIC). While the technique has been used for more than 20 years to ... Besides the analysis of polar compounds, HILIC can also be considered as a complementary technique to reversed-phase LC for the ... the ion exchange between a charged analyte and the stationary phase, and (iii) the adsorption of the analyte at the surface of ...
  http://www.chromatographyonline.com/hydrophilic-interaction-chromatography-characterization-therapeutic-monoclonal-antibodies-protein-pe
*  Exposure of the Main Italian River Basin to Pharmaceuticals
Aqueous samples were extracted by solid phase extraction (SPE) cartridges and analysed by reversed-phase liquid chromatography/ ... Chemical compounds with higher show greater affinity to hydrophobic matrices rather than to water. So, it might be predicted ... Sorbed analytes were then eluted with acetonitrile (10 mL) into a 10 mL glass test tube. The solvent was evaporated under a ... M. Stumpf, T. A. Ternes, R. D. Wilken, S. V. Rodrigues, and W. Baumann, "Polar drug residues in sewage and natural waters in ...
  https://www.hindawi.com/journals/jt/2011/989270/
*  GC-MS Sample Preparation | Thermo Fisher Scientific - FR
Learn how gas chromatography mass spectrometry samples are prepared for analysis using different preparation methods and GC-MS ... Reversed phase silica columns contain an alkyl-bonded phase for nonpolar to moderately polar compounds. The hydrophobic ... Normal phase silica phase chemistries These medium polarity sorbent columns are used in the isolation of polar analytes from ... Solid phase micro extraction (SPME): Used before both gas chromatography or HPLC, SPME is a sample extraction technique that ...
  https://www.thermofisher.com/fr/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/gas-chromatography-mass-spectrometry-gc-ms-information/gc-ms-sample-preparation.html
*  186008212 - XBridge Oligonucleotide BEH C18 OBD Prep Column, 130Å, 2.5 µm, 10 mm X 50 mm, 1/pkg to 667003977 - Empower 3...
This sorbent will provide reversed-phase cleanup of acidic, basic and neutral compounds form complex sample matrices. Oasis ... trifunctionally bonded amide phase that is stable from pH 2 to 11 to enable the separation of polar analytes spanning a wide ... reversed-phase chromatography. The availability of 1.7µm UPLC technology or 2.5µm HPLC particles in various column dimensions ... This sorbent will provide reversed-phase cleanup of acidic, basic and neutral compounds form complex sample matrices. Oasis ...
  http://www.waters.com/1/2/indexe22.html
*  Thermo Scientific HyperSep C8 Cartridges 500mg Bed Weight; 6mL Column
6mL Column Volume Chromatography:Solid Phase Extraction Products:SPE Columns, Discs, and Cartridges:SPE Columns ... Need a less retentive alternative to C18 for polar and nonpolar compounds? These hydrophobic reversed phase C8 cartridges offer ... HyperSep C8 SPE Cartridges provide excellent performance characteristics for moderately polar analytes. They also offer the ... Thermo Scientific HyperSep C8 Cartridges offer a less retentive alternative to C18 for polar and nonpolar compounds, and are ...
  https://www.fishersci.com/shop/products/hypersep-c8-cartridges-4/03251161
*  Thin-layer chromatography - Wikipedia
... when the stationary phase is polar and the mobile phase is non-polar, the method is normal-phase as opposed to reverse-phase. ... For instance, if normal-phase silica gel is used as the stationary phase, it can be considered polar. Given two compounds that ... Because different analytes ascend the TLC plate at different rates, separation is achieved. The mobile phase has different ... For example, with silica gel, a very polar substance, non-polar mobile phases such as heptane are used. The mobile phase may be ...
  https://en.wikipedia.org/wiki/Thin-layer_chromatography
*  New Chromatography Columns and Accessories for 2018 | LCGC
... including columns for reversed-phase, HILIC, chiral, SEC, GPC, ion-exchange, and SFC separations of small and large molecules. ... including columns for reversed-phase, HILIC, chiral, SEC, GPC, ion-exchange, and SFC separations of small and large molecules. ... Our annual review of new liquid chromatography columns and accessories, ... Our annual review of new liquid chromatography columns and accessories, ...
  http://www.chromatographyonline.com/new-chromatography-columns-and-accessories-2018
*  Hydrophilic interaction chromatography - Wikipedia
The more polar compounds will have a stronger interaction with the stationary aqueous layer than the less polar compounds. Thus ... HILIC uses hydrophilic stationary phases with reversed-phase type eluents. The name was suggested by Dr. Andrew Alpert in his ... October 2004). "Hydrophilic Interaction Chromatography Using Silica Columns for the Retention of Polar Analytes and Enhanced ... phases amide bonded phases cationic bonded phases zwitterionic bonded phases A typical mobile phase for HILIC chromatography ...
  https://en.wikipedia.org/wiki/Hydrophilic_interaction_chromatography
*  Difference between revisions of "Lidstrom:in vitro Pathway Assay: Sample Analysis via UPLC-MS/MS" - OpenWetWare
ion pairing: for when analytes include basic or ionic compounds that are difficult to chromatograph by reverse-phase LC. In IPC ... analytes are eluted in the order of their increasing polarity.The separation of very polar and/or ionic compoundsgenerally ... Ultrahigh performance liquid chromatography-tandem mass spectrometry method for fast and robust quantification of anionic and ... ion pairing: for when analytes include basic or ionic compounds that are difficult to chromatograph by reverse-phase LC. In IPC ...
  https://openwetware.org/wiki/?title=Lidstrom:in_vitro_Pathway_Assay:_Sample_Analysis_via_UPLC-MS/MS&diff=779349&oldid=624706