*  Background Prokaryotic plasmids have played out significant roles in the evolution - Suppression of KRas-mutant cancer through...
Even within the same cell, the genome sizes of different plasmids vary widely; for example, CT-43 has 10 plasmids with genome ... utilized network solutions to study all of the 2,343 plasmids with available genomic sequences and explained these plasmids ... The plasmids in this group display strain-dependent distribution, with some strains made up of no plasmids, whereas others have ... By analyzing the plasmids of genus group as a model to explore the development and dynamics of plasmids [20]. In the present ...
  http://complextraitgenomics.com/background-prokaryotic-plasmids-have-played-out-significant-roles-in-the-evolution/
*  Investigation of diversity of plasmids carrying the blaTEM-52 gene - DTU Orbit
Seven out of 10 blaTEM-52 plasmids tested in conjugation experiments were shown to be capable of self-transfer to a plasmid- ... Detected IncI1 plasmids underwent further plasmid multilocus sequence typing. RESULTS: RFLP profiles demonstrated dissemination ... Investigation of diversity of plasmids carrying the blaTEM-52 gene. Publication: Research - peer-review › Journal article - ... Some of the blaTEM-52 plasmids carry replicons that differ from the classical ones. Two novel replicons were detected, IncX1A ...
  http://orbit.dtu.dk/en/publications/investigation-of-diversity-of-plasmids-carrying-the-blatem52-gene
*  A versatile one-step CRISPR-Cas9 based approach to plasmid-curing | Microbial Cell Factories | Full Text
The pFREE plasmid was transformed into a strain harboring the same three pZ plasmids as used in Fig. 3. After recovery, plasmid ... Plasmid interference for curing antibiotic resistance plasmids in vivo. PLoS ONE. 2017;12:e0172913.View ArticleGoogle Scholar. ... The percentage of plasmid-carrying cells is depicted. Of the non-fluorescent and tested cells, all had lost the pFREE plasmid ... The percentage of plasmid-carrying cells is depicted. The pFREE plasmid was cured in all colonies tested. Curing of the target ...
  https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-017-0748-z
*  Patent US4403036 - Genetic reagents for generating plasmids containing multiple copies of DNA ... - Google Patents
The plasmid rings with modified restriction sites are also used in linear form to obtain head-to-tail joining of multiple DNA ... The modified plasmids permit cloning of DNA segments which can be recovered with sticky ends that are complementary but not ... The critical restriction site sequences of the modified plasmid rings can be prepared as a reagent which permits the sequence ... segments into plasmid rings for further cloning and/or expression of the DNA segment-directed protein. ...
  http://www.google.com/patents/US4403036?dq=7,172,682
*  Gentaur Molecular :Allele \ Plasmid Preparation for Retrovirus Packaging Service \ ABP-CS-RVPPP
Plasmid Preparation for Retrovirus Packaging Service \ ABP-CS-RVPPP for more molecular products just contact us ... trfA] Plasmid replication initiator protein TrfA. [pcnB b0143 JW5808] Poly(A) polymerase I (PAP I) (EC 2.7.7.19) (Plasmid copy ... Probable plasmid replicative DNA helicase (EC 3.6.4.12) (DnaB-like protein) (Protein P-3) (Virulence plasmid protein pGP1-D). [ ... Index / Allele / Plasmid Preparation for Retrovirus Packaging Service / Product Detail : ABP-CS-RVPPP Plasmid Preparation for ...
  http://www.antibody-antibodies.com/product_det.php?id=1193582&supplier=search&name=Plasmid%20Preparation%20for%20Retrovirus%20Packaging%20Service
*  Yeast Plasmid Miniprep - Yeast Plasmid Isolation Kit
... rescue plasmids from yeast using spin columns. Simple and highly efficient method. Zymolyase included. ... Yeast Plasmid Isolation Kit, Yeast Plasmid Mini-Preps. The Easy Yeast Plasmid Isolation Kit provides a simple and efficient ... Plasmid DNA mini-preps from yeast *Rescue positive prey plasmids after a yeast two-hybrid or a yeast one-hybrid screen ... A spin column is used to purify the plasmid DNA, which can then be transformed into E. coli for propagation and scaled-up ...
  http://www.clontech.com/US/Products/Protein_Interactions_and_Profiling/Yeast_Plasmid_Isolation_Kit/Plasmid_Minipreps?sitex=10020:22372:US&PROD=7IlmY5TCRajYCSlyCejDVPLR:S&PROD_pses=ZGF5A87DA390C37F08743F1349B41C41E8594AF7C1BA54BE52B9586B6BEC84715708865F6E3A9817E59DD50A75EB513675F91726325C66BAAA
*  uncut plasmid dna vs linearized plasmid gel - Molecular Cloning - BioForum
I am going to run a gel comparing my uncut plasmid dna vs linearized plasmid. My insert is 135 bps and my vector is 3Kb. What ... I am assuming the uncut plasmid will have several bands at different sizes and the linearized will have only one, is that right ... uncut plasmid dna vs linearized plasmid gel - posted in Molecular Cloning: Hello, ... I am going to run a gel comparing my uncut plasmid dna vs linearized plasmid. My insert is 135 bps and my vector is 3Kb. What ...
  http://www.protocol-online.org/forums/topic/19386-uncut-plasmid-dna-vs-linearized-plasmid-gel/
*  The IncP-1 plasmid backbone adapts to different host bacterial species and evolves through homologous recombination.
Globally circulating IncP-1 plasmids hence contain mosaic structures of segments derived from several parental plasmids that ... Phylogenetic analysis reveals seven clades, in which two plasmids that we isolated from a marine biofilm represent a novel ... We also found that homologous recombination is a prominent feature of the plasmid backbone evolution. Analysis of genomic ... Although these inserted elements have drawn considerable interest, evolutionary information about the plasmid backbones, which ...
  http://publications.lib.chalmers.se/publication/169522-the-incp-1-plasmid-backbone-adapts-to-different-host-bacterial-species-and-evolves-through-homologou
*  Replication and segregation of a miniF plasmid during the division cycle of Escherichia coli
It is concluded that both pML31 and pBR322 plasmids can replicate at all stages of the division cycle, with a probability of ... Replication and segregation of a miniF plasmid during the division cycle of Escherichia coli. ... In contrast, the modes of segregation of pML31 and pBR322 plasmids into daughter cells at division appeared to differ, raising ... Replication of the miniF plasmid pML31 was examined during the division cycle of Escherichia coli growing with doubling times ...
  https://repository.lib.fit.edu/handle/11141/1675
*  Difference between revisions of "Vectors" - OpenWetWare
PlasMapper: "automatically generates and annotates plasmid maps using only the plasmid DNA sequence as input. Plasmid sequences ... automatically generates and annotates plasmid maps using only the plasmid DNA sequence as input. Plasmid sequences up to 20,000 ... automatically generates and annotates plasmid maps using only the plasmid DNA sequence as input. Plasmid sequences up to 20,000 ... For some plasmids (pMB1/colE1 replicons) the positive regulator is an RNA and in others (e.g. pSC101) it is a protein. Plasmids ...
  https://openwetware.org/wiki/?title=Vectors&diff=9103&oldid=9089
*  Transcriptional reprogramming in yeast using dCas9 and combinatorial gRNA strategies | Microbial Cell Factories | Full Text
For generation of multiplex gRNA plasmid pERA-103, plasmid pERA-86 served as template for primers EDJ-216 and EDJ-217, plasmid ... To generate the multiplex gRNA plasmid pERA-104, plasmid pERA-86 served as template for primers EDJ-216 and EDJ-217, plasmid ... For generation of multiplex gRNA plasmid pERA-105, plasmid pERA-95 served as template for primers EDJ-216 and EDJ-217, plasmid ... BsmI pre-digested USER plasmid p0054 [47], generating plasmids pERA-39 and pERA-40, respectively. Plasmids pEDJ-51 and pEDJ-52 ...
  https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-017-0664-2
*  LARGE PLASMIDS
... the End jgraham at bronze.ucs.indiana.edu Fri Dec 11 10:10:11 EST 1992 *Previous message: LARGE PLASMIDS ... Ian, I've had much luck sith a 11.5 Kb low copy (pSC101) plasmid. I grow in TB 'terrific broth' as described in the new ...
  http://www.bio.net/bionet/mm/methods/1992-December/003761.html
*  Planning Plasmids | Science
... and design complex plasmids for molecular biologists. ... Planning Plasmids Message Subject. (Your Name) has forwarded a ...
  http://science.sciencemag.org/content/286/5444/1495
*  Plasmid-mediated resistance - Wikipedia
Plasmid-mediated resistance is the transfer of antibiotic resistance genes which are carried on plasmids. The plasmids can be ... Other smaller plasmids (usually < 10 kb in size) can be mobilized by a conjugative plasmid (usually > 30 kb) in order to be ... are common for resistance plasmids in Enterobacteriaceae. Often multiple beta-lactamase genes are found on the same plasmid ... It is very common for the resistance genes or entire resistance cassettes to be re-arranged on the same plasmid or be moved to ...
  https://en.wikipedia.org/wiki/Plasmid-mediated_resistance
*  plasmid sequences
... Dominic-Luc Webb molmed domweb at mbox.ki.se Fri Jul 13 03:43:17 EST 2001 *Previous message: Shine Dalgano ... Does anyone know of a database with plasmid sequences? Alternatively, I would like to know vendor for pCMX plasmid. Cheers, ... I am trying to find the sequence of some plasmids, but I have no idea who the vendor is, etc... ...
  http://www.bio.net/bionet/mm/proteins/2001-July/009549.html
*  Plasmid concentration
... Cristina Cebrian cebrian at ar.vhebron.es Thu Sep 18 14:10:41 EST 1997 *Previous message: spermine/ ... Just wandering... Is it possible to know plasmid DNA concentration without digestion? Is the 260 absorvance reliable? is it ...
  http://www.bio.net/bionet/mm/methods/1997-September/061078.html
*  Plasmid - Journal - Elsevier
Plasmid publishes original research on genetic elements in all kingdoms of life with emphasis on maintenance, transmission and ... Plasmids promote the immigration of other plasmids but repress co-colonizing plasmids. ... Plasmids promote the immigration of other plasmids but repress co-colonizing plasmids. ... Recently published articles from Plasmid * Evolution in situ of ARI-A in pB2-1, a type 1 IncC plasmid recovered from Klebsiella ...
  https://www.journals.elsevier.com/plasmid/
*  Plasmid tracker - opinions?
... Andy Phillips andy.phillips at bbsrc.ac.uk Wed Oct 16 03:16:56 EST 1996 *Previous message: ... There was an advert for Plasmid Tracker on this newsgroup recently. Has anyone used this program? Is useful, stable etc? I'm ...
  http://www.bio.net/bionet/mm/bio-soft/1996-October/015314.html
*  plasmid DNA purification
... Claude Baker claude at mcs.net Mon Feb 20 21:17:15 EST 1995 *Previous message: Help: PAGE sequencing ... Does anyone know if any really good Plasmid DNA purifications kits available on the market? Looking for max DNA recovery. ...
  http://www.bio.net/bionet/mm/methods/1995-February/024803.html
*  DNA, plasmid center ?
... GIETZ at bldghsc.lan1.umanitoba.ca GIETZ at bldghsc.lan1.umanitoba.ca Fri Oct 8 11:18:00 EST 1993 * ... question about the most efficient way to get yeast libraries and plasmids. ,The Arabidopsis community has a DNA stock center ... These are made from the plasmid pBluescript. Good Luck with your project! Dan Gietz ______________________________________ R. ...
  http://www.bio.net/bionet/mm/yeast/1993-October/000368.html
*  Plasmid drawing program
... Yii-Cheng Chou ycchou at life.nthu.edu.tw Fri Mar 18 03:24:22 EST 1994 *Previous message: Plasmid ...
  http://www.bio.net/bionet/mm/bio-soft/1994-March/007149.html
*  Concatemerized Plasmid DNA
So, how can this be happening? Could , the cells have become contaminated with a rec containing plasmid? Is there , any ... Concatemerized Plasmid DNA. Geoffrey Kidd gkidd at aptagen.com Wed Nov 11 15:11:41 EST 1998 *Previous message: type II ... www.aptagen.com Antonin Tutter wrote: , I have encountered the problem of concatemerized plasmid DNA in recent , preparations ...
  http://www.bio.net/bionet/mm/methods/1998-November/071629.html
*  plasmid rescue experiments
... Department of Molecular Karma YABLONSKY at BIOVAX.RUTGERS.EDU Thu May 24 12:59:00 EST 1990 * ... These control plasmids are also masked. The effect seems to be due to the fact that relatively high amounts (10s of ng ) of ... I'm experiencing a problem with a plasmid rescue experiment. It appears that the overwhelming amount of host cell DNA isolated ... If anyone has any experience in plasmid rescue or some other enlightening comments please e-mail me directly. Thank you, ...
  http://www.bio.net/bionet/mm/bioforum/1990-May/002542.html
*  plasmid library amplification
... Franck Laurent franck.laurent at medew.fyto.wau.nl Wed Nov 27 08:38:23 EST 1996 *Previous ... Hi Does anyone has a good protocol and experience to amplify a plasmid library? Thanks Franck franck.laurent at medew.fyto.wau. ...
  http://www.bio.net/bionet/mm/methods/1996-November/051945.html