Tyrosine-protein kinase SYK/ZAP-70, inter-SH2 domain superfamily (IPR023420) | InterPro | EMBL-EBI
We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their ... InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites ... Protein phosphorylation, which plays a key role in most cellular activities, is a reversible process mediated by protein ... Tyrosine-protein kinases can transfer a phosphate group from ATP to a tyrosine residue in a protein. These enzymes can be ...http://www.ebi.ac.uk/interpro/entry/IPR023420
Lymphocyte cytosolic protein 2 - Wikipedia
"Differential Requirements for Src-Family Kinases in SYK or ZAP70-Mediated SLP-76 Phosphorylation in Lymphocytes". Frontiers in ... activation-induced tyrosine phosphorylation and recruitment of SLP-76 to Vav by distinct isoforms of the CD45 protein-tyrosine ... activation-induced tyrosine phosphorylation and recruitment of SLP-76 to Vav by distinct isoforms of the CD45 protein-tyrosine ... "SLP-76 is a substrate of the high affinity IgE receptor-stimulated protein tyrosine kinases in rat basophilic leukemia cells". ...https://en.wikipedia.org/wiki/Lymphocyte_cytosolic_protein_2
TXK (gene) - Wikipedia
Tyrosine-protein kinase TXK is an enzyme that in humans is encoded by the TXK gene. GRCh38: Ensembl release 89: ENSG00000074966 ... 1996). "Phosphorylation of SLP-76 by the ZAP-70 protein-tyrosine kinase is required for T-cell receptor function". J. Biol. ... "A YAC contig spanning a cluster of human type III receptor protein tyrosine kinase genes (PDGFRA-KIT-KDR) in chromosome segment ... a novel human tyrosine kinase expressed in T cells shares sequence identity with Tec family kinases and maps to 4p12". Hum Mol ...https://en.wikipedia.org/wiki/TXK_(gene)
ZAP70 - Wikipedia
... spectrometry of the sites of tyrosine phosphorylation induced in activated Jurkat T cells on the protein tyrosine kinase ZAP-70 ... Katzav S, Sutherland M, Packham G, Yi T, Weiss A (December 1994). "The protein tyrosine kinase ZAP-70 can associate with the ... Huby RD, Carlile GW, Ley SC (December 1995). "Interactions between the protein-tyrosine kinase ZAP-70, the proto-oncoprotein ... and it is a member of the protein-tyrosine kinase family. ZAP-70 in B cells is used as a prognostic marker in identifying ...https://en.wikipedia.org/wiki/ZAP70
NF-κB - Wikipedia
... protein kinase Lck is recruited and phosphorylates the ITAMs of the CD3 cytoplasmic tail. ZAP70 is then recruited to the ... "Tyrosine phosphorylation-dependent activation of NF-kappa B. Requirement for p56 LCK and ZAP-70 protein tyrosine kinases". ... "DNA binding of repressor nuclear factor-kappaB p50/p50 depends on phosphorylation of Ser337 by the protein kinase A catalytic ... Through a cascade of phosphorylation events, the kinase complex is activated and NF-κB is able to enter the nucleus to ...https://en.wikipedia.org/wiki/NF-%CE%BAB
Evidence That Glutathione Depletion Is a Mechanism Responsible for the Anti-Inflammatory Effects of Ethyl Pyruvate in Cultured...
Tyrosine phosphorylation-dependent activation of NF-κB: requirement for p56 LCK and ZAP-70 protein tyrosine kinases. Eur J ... using T4 polynucleotide kinase (Promega). Nuclear proteins (5 μg) were incubated with γ-32P-labeled NF-κB probe at room ... Protein concentration was determined using the Bio-Rad protein assay kit with BSA as a standard. Results are expressed as nmol/ ... Nuclear protein concentration was determined using the Bio-Rad protein assay kit with BSA as a standard (Bio-Rad Laboratories, ...http://jpet.aspetjournals.org/content/308/1/307.long
Mutagenetix > Phenotypic Mutation ...
FUNCTION: This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of ... Phosphorylation of SLP-76 by the ZAP-70 Protein-Tyrosine Kinase is Required for T-Cell Receptor Function. J Biol Chem. 271, ... is a protein tyrosine kinase (PTK) that binds to the doubly phosphorylated immunoreceptor tyrosine-based activation motifs ( ... Protein Prediction. Figure 7. Structure of ZAP-70. Mouse Zap-70 is a 618 amino acid protein tyrosine kinasen (PTK) that ...http://mutagenetix.utsouthwestern.edu/phenotypic/phenotypic_rec.cfm?pk=1084
... spectrometry of the sites of tyrosine phosphorylation induced in activated Jurkat T cells on the protein tyrosine kinase ZAP-70 ... By quantitatively assessing the tyrosine phosphorylation status of activated kinases in xenograft models of ALL, we were able ... Using an unbiased and quantitative tool to identify, the tyrosine phosphorylation state of activated kinases in xenograft ... 4A and B) was markedly upregulated only in ALL-2 along with phosphorylation of key downstream tyrosine phosphorylation targets ...http://cancerres.aacrjournals.org/content/76/9/2766
Nonmitogenic Anti-CD3 Monoclonal Antibodies Deliver a Partial T Cell Receptor Signal and Induce Clonal Anergy | JEM
... including the protein tyrosine kinase, ZAP70 ((9)). In the present study, the ability of nonmitogenic anti-CD3 to trigger these ... 1996) Lck regulates the tyrosine phosphorylation of the T cell receptor subunits and ZAP-70 in murine thymocytes. J Exp Med 183 ... additional tyrosine phosphorylated proteins, including the ZAP-70 kinase, associate with the TCR-CD3 complex ((11)). These ... including ζ chain tyrosine phosphorylation and ZAP-70 association. However, unlike the mitogenic anti-CD3 stimulation, ...http://jem.rupress.org/content/185/8/1413
The immunological synapse balances T cell receptor signaling and degradation. - Nuffield Department of Orthopaedics,...
Phosphorylation, Models, Immunological, Computer Simulation, ZAP-70 Protein-Tyrosine Kinase, Protein-Tyrosine Kinases ... Adaptor Proteins, Signal Transducing, Proteins, Cytoskeletal Proteins, Receptors, Antigen, T-Cell, Antigens, Ligands, ...https://www.ndorms.ox.ac.uk/publications/482638
Similar articles for PubMed (Select 14738763) - PubMed - NCBI
Regulation of T-cell antigen receptor signalling by Syk tyrosine protein kinase. ... Hematopoietic cell phosphatase (HCP) regulates p56LCK phosphorylation and ZAP-70 binding to T cell receptor zeta chain. ... The Sts proteins target tyrosine phosphorylated, ubiquitinated proteins within TCR signaling pathways. ... Tyrosine 474 of ZAP-70 is required for association with the Shc adaptor and for T-cell antigen receptor-dependent gene ...https://www.ncbi.nlm.nih.gov/pubmed?LinkName=pubmed_pubmed&from_uid=14738763
Vorinostat interferes with the signaling transduction pathway of T-cell receptor and synergizes with phosphoinositide-3 kinase...
... spectrometry of the sites of tyrosine phosphorylation induced in activated Jurkat T cells on the protein tyrosine kinase ZAP-70 ... ZAP70 tyrosine kinase is recruited to the TCR where it is activated by LCK through tyrosine phosphorylation. ZAP70 is a key ... Tyrosine phosphorylation of ZAP70 correlates well with its increased kinase activity and downstream signaling events. To extend ... 2002) Protein kinase C and AKT/protein kinase B in CD4+ T-lymphocytes: new partners in TCR/CD28 signal integration. Mol Immunol ...http://www.haematologica.org/content/95/4/613
Mutagenetix > Phenotypic Mutation ...
Three major kinases, Lck, Zap70, and Itk, regulate tyrosine phosphorylation during the early phases of TCR signaling. Treatment ... non-catalytic region of tyrosine kinase adaptor protein), IL-2-induced tyrosine kinase (Itk), PLC-γ1, adhesion and ... and protein tyrosine binding domain-containing proteins. The Syk family kinases phosphorylate LAT and SLP-76. LAT binds to the ... non-catalytic region of tyrosine kinase adaptor protein), Itk, PLCγ1, adhesion and degranulation-promoting adaptor protein ( ...http://mutagenetix.utsouthwestern.edu/phenotypic/phenotypic_rec.cfm?pk=1009&rn=99&rl=511&so=al&ac=1&r0=0&nr=100
CD25 and CD69 induction by α4β1 outside-in signalling requires TCR early signalling complex proteins | Biochemical Journal
1994) The protein tyrosine kinase ZAP-70 can associated with the SH2 domain of proto-Vav. J. Biol. Chem. 269:32579-32585. ... 1999) Tyrosine 319, a newly identified phosphorylation site of ZAP-70, plays a critical role in T cell antigen receptor ... 2000) CD28 and the tyrosine kinase lck stimulate mitogen-activated protein kinase activity in T cells via inhibition of the ... 1996) Mutation of tyrosines 492/493 in the kinase domain of ZAP-70 affects multiple T-cell receptor signaling pathways. J. Biol ...http://www.biochemj.org/content/454/1/109
TFG (gene) - Wikipedia
... mitogen-activated kinase activity through a pathway that involves related adhesion focal tyrosine kinase and protein kinase C ... Gotoh N, Toyoda M, Shibuya M (Apr 1997). "Tyrosine phosphorylation sites at amino acids 239 and 240 of Shc are involved in ... Walk SF, March ME, Ravichandran KS (Aug 1998). "Roles of Lck, Syk and ZAP-70 tyrosine kinases in TCR-mediated phosphorylation ... receptor is coupled to mitogen-activated protein kinase via protein kinase C and epidermal growth factor receptor kinase". The ...https://en.wikipedia.org/wiki/TFG_(gene)
Anti-LAT Antibody, clone 2E9 | 05-561
... domain of the Tec family protein tyrosine kinase, Inducible T cell Kinase (ITK), is critical for the recruitment of the kinase ... Present in T-cells but not B-cells (at protein level).. PTM: Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by ... Deduced amino acid sequence identifies a novel integral membrane protein containing multiple potential tyrosine phosphorylation ... Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular ...http://www.merckmillipore.com/INTL/en/product/Anti-LAT-Antibody-clone-2E9,MM_NF-05-561
Frontiers | Protein Kinase C-θ (PKC-θ) in Natural Killer Cell Function and Anti-Tumor Immunity | Immunology
The protein kinase C-theta (PKCtheta), which is essential for T cell function and survival, is also required for efficient anti ... The protein kinase C-theta (PKCtheta), which is essential for T cell function and survival, is also required for efficient anti ... Anel, A., and Kleinfeld, A. M. (1993). Tyrosine phosphorylation of a 100 kDa protein is correlated with cytotoxic T-lymphocyte ... Chan, A. C., Iwashima, M., Turck, C. W., and Weiss, A. (1992). ZAP-70: a 70 kd protein-tyrosine kinase that associates with the ...https://www.frontiersin.org/articles/10.3389/fimmu.2012.00187/full
The Nonphagocytic NADPH Oxidase Duox1 Mediates a Positive Feedback Loop During T Cell Receptor Signaling | Science Signaling
... especially phosphorylation of tyrosine-319 of ζ chain-associated protein kinase of 70 kilodaltons (ZAP-70), store-operated ... protein tyrosine phosphatase 2 promoted the phosphorylation of ZAP-70 and its association with the Src family tyrosine kinase ... Reactive oxygen species enhance T cell receptor signaling by promoting the phosphorylation of a proximal kinase. ... Reactive oxygen species enhance T cell receptor signaling by promoting the phosphorylation of a proximal kinase. ...http://stke.sciencemag.org/content/3/133/ra59
Linker for Activation of T cells - Wikipedia
The protein encoded by this gene is phosphorylated by ZAP70/Syk protein tyrosine kinases following activation of the T-cell ... Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular ... "SLP-76 is a substrate of the high affinity IgE receptor-stimulated protein tyrosine kinases in rat basophilic leukemia cells". ... Sarkar S (1999). "Tyrosine phosphorylation and translocation of LAT in platelets". FEBS Lett. 441 (3): 357-60. doi:10.1016/ ...https://en.wikipedia.org/wiki/Linker_for_Activation_of_T_cells
Actin and agonist MHC-peptide complex-dependent T cell receptor microclusters as scaffolds for signaling
Tyrosine 319, a newly identified phosphorylation site of ZAP-70, plays a critical role in T cell antigen receptor signaling. J ... We next determined the relationship of microcluster formation to activation of tyrosine kinases Lck and ζ-associated protein of ... The Src-selective kinase inhibitor PP1 also inhibits Kit and Bcr-Abl tyrosine kinases. J. Biol. Chem. 278:4847-4853. [PubMed] ... in which the cSMAC has relatively low tyrosine kinase activation and the pSMAC contains foci of efficient tyrosine kinase ...http://pubmedcentralcanada.ca/pmcc/articles/PMC1373686/
Keeping the (Kinase) Party Going: SLP-76 and ITK Dance to the Beat | Science Signaling
The Src-family tyrosine kinase Lck and the Syk-family tyrosine kinase ζ chain-associated protein kinase of 70 kD (ZAP-70), ... together with ITK, are necessary for the phosphorylation of PLC-γ1 in response to TCR stimulation. ITK is thought to ... The Tec-family protein tyrosine kinase IL-2-inducible T cell kinase (ITK) mediates T cell activation, as does the adaptor ... The adaptor protein SLP-76 serves as more than a neutral adaptor during T cell activation. ...http://stke.sciencemag.org/content/2007/396/pe39
CD4 - Wikipedia
Phosphorylated ITAM motifs on CD3 recruit and activate SH2 domain-containing protein tyrosine kinases (PTK) such as Zap70 to ... further mediate downstream signalling through tyrosine phosphorylation, leading to transcription factor activation including NF ... allows the tyrosine kinase Lck bound to the cytoplasmic tail of CD4 to tyrosine-phosphorylate the Immunoreceptor tyrosine ... "The CD4 receptor is complexed in detergent lysates to a protein-tyrosine kinase (pp58) from human T lymphocytes". Proc. Natl. ...https://en.wikipedia.org/wiki/CD4
A Novel Role for p21-Activated Protein Kinase 2 in T Cell Activation | The Journal of Immunology
The adaptor protein Nck links receptor tyrosine kinases with the serine-threonine kinase Pak1. J. Biol. Chem. 271:20997. ... Phosphorylation of myosin light chain kinase by p21-activated kinase PAK2. J. Biol. Chem. 275:18366. ... such as ZAP70, Syk, Lck, and phospholipase C (PLC)γ1 (2). The fourth most frequently represented cDNA was PAK2. Two kinase ... 4 Abbreviations used in this paper: MAPK, mitogen-activated protein kinase; PAK, p21-activated serine/threonine protein kinase ...http://www.jimmunol.org/content/172/12/7324.long
Fyn-Binding Protein (Fyb)/Slp-76-Associated Protein (Slap), Ena/Vasodilator-Stimulated Phosphoprotein (Vasp) Proteins and the...
TCR engagement induces the activation of tyrosine kinases (Fyn, Lck, and ZAP-70), leading to the phosphorylation of several ... Tyrosine phosphorylation is a key step during T cell activation. At least two of the proteins involved in the complex, Fyb/SLAP ... 1994) In vivo association of Grb2 with pp116, a substrate of the T cell antigen receptor-activated protein tyrosine kinase. J. ... 1995), and a protein of 120-130 kD named Fyn-binding protein (Fyb) or SLP-76-associated protein of 130 kD (SLAP-130), which was ...http://jcb.rupress.org/content/149/1/181.long
TUBA4A - Wikipedia
Huby RD, Carlile GW, Ley SC (Dec 1995). "Interactions between the protein-tyrosine kinase ZAP-70, the proto-oncoprotein Vav, ... induces tyrosine phosphorylation of alpha-tubulin and its association with the SH2 domain of the p59fyn protein tyrosine kinase ... Tubulin alpha-4A chain is a protein that in humans is encoded by the TUBA4A gene. Microtubules of the eukaryotic cytoskeleton ... Kirsch J, Langosch D, Prior P, Littauer UZ, Schmitt B, Betz H (Nov 1991). "The 93-kDa glycine receptor-associated protein binds ...https://en.wikipedia.org/wiki/TUBA4A