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*  Glycosylation - Wikipedia
N-linked glycosylation requires participation of a special lipid called dolichol phosphate. O-linked glycans attached to the ... In addition to their function in protein folding and cellular attachment, the N-linked glycans of a protein can modulate a ... In other cases, proteins are not stable unless they contain oligosaccharides linked at the amide nitrogen of certain asparagine ... Five classes of glycans are produced: N-linked glycans attached to a nitrogen of asparagine or arginine side-chains. ...
  https://en.wikipedia.org/wiki/Glycosylation
*  Similarities and Differences in the Glycosylation Mechanisms in Prokaryotes and Eukaryotes
... the pglB protein of C. jejuni is capable of transferring a variety of O-antigen oligosaccharides onto protein acceptors in ... donated by dolichol-phosphate-linked sugars, to form Glc3Man9GlcNAc2-P-P-Dol (Figure 1(b)). The glucoses play a pivotal role in ... and the glycans are transferred en bloc by the O-OSTs from the LLOs carrier onto the protein [39]. The translocation of the LLO ... "Glycosylation of the phosphate binding protein, PstS, in Streptomyces coelicolor by a pathway that resembles protein O- ...
  https://www.hindawi.com/journals/ijmicro/2010/148178/
*  User-friendly extraction and multistage tandem mass spectrometry based analysis of lipid-linked oligosaccharides in microalgae ...
Background Protein N-glycosylation is initiated within the endoplasmic reticulum through the synthesis of a lipid-linked ... oligosaccharides (LLO) precursor. This precursor is then transferred en bloc... ... Isomeric analysis of oligomannosidic N-glycans and their dolichol-linked precursors. Glycobiology. 2012;22:389-99.CrossRef ... Protein N-glycosylation is initiated within the endoplasmic reticulum through the synthesis of a lipid-linked oligosaccharides ...
  https://link.springer.com/article/10.1186%2Fs13007-018-0374-8
*  DiVA - Search result
... of the target oligosaccharides were synthesized as amino-spacer glycosides in order to make conjugation to a carrier protein ... phosphate and the transesterification of 2-hydroxypropyl p-nitrophenyl phosphate, which are model systems for the same ... Carbohydrates, or glycans, are ubiquitous biomolecules exhibiting a wide range of biological roles. The specific functions of ... Different matrices and additives for positive ion FAB-MS of model polyprenol and dolichol compounds were investigated. Negative ...
  http://www.diva-portal.org/smash/resultList.jsf?dswid=-6003&af=%5B%22publicationTypeCode%3AcomprehensiveDoctoralThesis%22%2C%22categoryId%3A11533%22%5D&p=1&fs=true&language=en&searchType=SIMPLE&query=&aq=%5B%5B%7B%22organisationId%22%3A%22631%22%7D%5D%5D&aq2=%5B%5B%5D%5D&aqe=%5B%5D&noOfRows=50&sortOrder=author_sort_asc&sortOrder2=title_sort_asc&onlyFullText=false&sf=all
*  Glycomic analysis: an array of technologies
Probing protein-carbohydrate interactions with microarrays of synthetic oligosaccharides. Chembiochem. 2004;5:379-382. [PubMed] ... and starts with the transfer of the core glycan GlcNAc2Man9Glc3 from a dolichol phosphate precursor to the asparagine residue. ... To understand the role of glycans in modulating protein and lipid function, it is necessary to identify the molecular context ... Local glycan concentration is determined by both the mutivalent presentation of glycans within a single protein and the ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3443568/?lang=en-ca
*  N-Glycan biosynthesis | LAMP
... and involved in conversion of dolichol phosphate from dolichol (dolichol kinase) and dolichol diphosphate ( ... Two different types of glycans attached to proteins are N-glycans and O-glycans. N-glycans, more precisely N-linked glycans are ... Dolichyl-phosphate beta-glucosyltransferase (ALG5). 2.4.1.117. TGME49_216540. Dolichyl-diphosphooligosaccharide-protein ... The term 'Glycan' refers to oligosaccharides or polysaccharides. They also refer to glycoconjugates such as glycolipids and ...
  http://www.llamp.net/?q=N-Glycan%20biosynthesis
*  Frontiers | Archaeal S-Layers: Overview and Current State of the Art | Microbiology
A paracrystalline protein surface layer, commonly referred to as S-layer, is present in nearly all archaea described to date. S ... A paracrystalline protein surface layer, commonly referred to as S-layer, is present in nearly all archaea described to date. S ... AglR flips the mannose bound dolichol phosphate carrier and AglS adds the final mannose to the protein (based on the model ... Interestingly, UDP activated oligosaccharides have been detected in the biosynthesis process of pseudomurein in other ...
  https://www.frontiersin.org/articles/10.3389/fmicb.2017.02597/full
*  Glycosylation - Wikipedia
In addition to their function in protein folding and cellular attachment, the N-linked glycans of a protein can modulate a ... N-linked glycosylation requires participation of a special lipid called dolichol phosphate. ... Glycan length-can be short- or long-chain oligosaccharides. Mechanisms[edit]. There are various mechanisms for glycosylation, ... phosphoglycans linked through the phosphate of a phosphoserine;. *C-linked glycans, a rare form of glycosylation where a sugar ...
  https://en.wikipedia.org/wiki/Glycosylated
*  new enzymes
... phosphate from 4-(phosphohydroxy)-L-threonine and 1-deoxy-D-xylulose-5-phosphate by PdxA and PdxJ protein. FEBS Lett. 449 (1999 ... 2. Elharar, Y., Podilapu, A.R., Guan, Z., Kulkarni, S.S. and Eichler, J. Assembling glycan-charged dolichol phosphates: ... Comments: The enzyme catalyses the addition of N-acetyl-α-D-glucosamine to the core 2 structure of O-glycans.. Links to other ... and O-nitrophenyl glycosides of oligosaccharides. Analysis of substrates and products for four N-acetyl-D-glucosaminyl- ...
  http://www.sbcs.qmul.ac.uk/iubmb/enzyme/supplements/sup2018/newenz3.html
*  V Region Carbohydrate and Antibody Expression | The Journal of Immunology
It has been proposed that glycans located on exposed protein loops, rather than sequestered within β-pleated sheets, will be ... When Abs possessed oligosaccharides in the V region of both H and L chains, the H chain carbohydrates remained in the high ... A dolichol (lipid)-linked precursor carbohydrate consisting of a branched GlcNAc2Mann9Glc3 structure is transferred from the ER ... phosphate gels or under reducing conditions on 12.5% Tris-glycine (TG) gels, as described (16). Samples prepared for ...
  http://www.jimmunol.org/content/172/9/5489.full
*  N-linked glycosylation - Wikipedia
Sugar molecules are attached to the dolichol through a pyrophosphate linkage (one phosphate was originally linked to dolichol, ... The nature of N-linked glycans attached to a glycoprotein is determined by the protein and the cell in which it is expressed. ... often almost as many as are seen in the precursor oligosaccharides before it is attached to the protein. Complex ... A chaperone protein (calnexin/calreticulin) binds to the unfolded or partially folded protein to assist protein folding. The ...
  https://en.wikipedia.org/wiki/N-linked_glycosylation
*  glycosylphosphatidylinositols
N-glycans, not the GPI anchor, mediate the apical targeting of a naturally glycosylated, GPI-anchored protein in polarised ... A homologue of Saccharomyces cerevisiae Dpm1p is not sufficient for synthesis of dolichol-phosphate-mannose in mammalian cells ... Synthetic Oligosaccharides for Probing Infection and Innate Immunity. Geert Jan Boons; Fiscal Year: 2013 ... Effect of glycans and the glycophosphatidylinositol anchor on strain dependent conformations of scrapie prion protein: improved ...
  http://www.labome.org/topics/chemicals/lipids/glycolipids/glycosylphosphatidylinositols-13367.html
*  Glycan - Wikipedia
N-linked glycans also contribute to protein folding by steric effects. For example, cysteine residues in the peptide may be ... The modification of an N-linked glycan with a mannose-6-phosphate residue serves as a signal that the protein to which this ... First, two N-acetyl glucosamine residues are attached to dolichol monophosphate, a lipid, on the external side of the ... Hase S, Ikenaka T, Matsushima Y (November 1978). "Structure analyses of oligosaccharides by tagging of the reducing end sugars ...
  https://en.wikipedia.org/wiki/Glycan
*  PRODUCTION OF GLYCOPROTEINS WITH REDUCED O-GLYCOSYLATION - Patent application
Therefore, the only glycans on the Kringle 1-3 protein would be the result of O-glycosylation. [0082] Plasmid containing DNA ... addition of the initial mannose from dolichol-phosphate mannose to a serine or threonine residue of a nascent glycoprotein in ... 0005] In general, the glycosylation structures of glycoprotein oligosaccharides will vary depending upon the host species of ... protein in the cell or one or more α 1,2-mannosidases, or both, at the time expression of the protein is induced. The protein ...
  http://www.patentsencyclopedia.com/app/20120237973
*  Frontiers | A comparative glycoproteome study of developing endosperm in the hexose-deficient miniature1 (mn1) seed mutant and...
... using 2D gel electrophoresis and glycosylation/total protein staining, followed by image analysis. Protein identification was ... using 2D gel electrophoresis and glycosylation/total protein staining, followed by image analysis. Protein identification was ... These proteins and activities were related to endoplasmic reticulum (ER) stress and unfolded protein response (UPR) as a result ... Of these, 45 showed > 20% decrease/increase in glycosylation level and were selected for protein identification. A large number ...
  https://www.frontiersin.org/articles/10.3389/fpls.2014.00063/full
*  ASMscience | Campylobacter Metabolomi
... characterized novel biosynthetic substrates and novel flagellar glycans, and elucidated poorly understood metabolic pathways. ... Lehrman, M. A. 2007. Teaching dolichol-linked oligosaccharides more tricks with alternatives to metabolic radiolabeling. ... on the polyprenyl carrier dolichol phosphate. It is then flipped to the luminal side of the ER, where it is transferred to ... Szymanski, C. M.,, S. M. Logan,, D. Linton, and, B. W. Wren. 2003a. Campylobacter -a tale of two protein glycosylation systems. ...
  http://www.asmscience.org/content/book/10.1128/9781555815554.ch29
*  Altered glycosylation of exported proteins, including surface immune receptors, compromises calcium and downstream signaling...
Analysis of dolichol-linked oligosaccharides. a HPLC-based size fractionation of 2-pyridylamine (PA)-labelled oligosaccharides ... 50 mM sodium phosphate pH 7.5, 1 % NP-40) or with 1 μL Endo Hf (Endoglycosidase H fused to maltose binding protein) in G5 ... Arabidopsis thaliana ALG3 mutant synthesizes immature oligosaccharides in the ER and accumulates unique N-glycans. Glycobiology ... Protein extraction and western blotting. For protein extraction from mature plants or seedlings, frozen leaf discs or seedlings ...
  https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-016-0718-3
*  Patente US7507417 - Immunogenic compositions comprising human immunodeficiency virus type 2 (HIV ... - Google Patentes
tunicamycin inhibits assembly of dolichol-P-P glycan, and for this reason the 80 Kd protein does not become glycosylated. ... Asparagine-linked oligosaccharides (Glc3ManqGlcNAc2) of glycoproteins undergo extensive modifications or processing following ... All glycoproteins carrying N-linked glycans derive their oligosaccharide moiety from the lipid-linked oligosaccharide, Glc3Manq ... such as aluminum phosphate and aluminum hydroxide gel, in an amount sufficient to potentiate humoral or cell-mediated immune ...
  http://www.google.es/patents/US7507417
*  N-Glycan biosynthesis | LAMP
Dolichol kinase. 2.7.1.108. CMU_013270. UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephospho transferase (ALG7 ... Two different types of glycans attached to proteins are N-glycans and O-glycans. N-glycans, more precisely N-linked glycans are ... Dolichyl-phosphate beta-glucosyltransferase (ALG5). 2.4.1.117. Missing in annotation. Dolichyl-diphosphooligosaccharide-protein ... The term 'Glycan' refers to oligosaccharides or polysaccharides. They also refer to glycoconjugates such as glycolipids and ...
  http://www.llamp.net/?q=Cmu%20N-Glycan%20biosynthesis
*  VIVO ISOTOPIC LABELING METHOD FOR QUANTITATIVE GLYCOMICS - Patent application
The glycans are released from the digested protein and/or lipid using techniques which are standard in the art (e.g., PNGaseF ... For N-linked oligosaccharides, a 14-sugar precursor (dolichol) is first added to the asparagine in the polypeptide chain of the ... 1A). The first and rate-limiting step in this pathway converts fructose-6-phosphate to glucosamine-6-phosphate with the ... 0033]The analysis of glycans can proceed on the isolated glycans or on the proteins or lipids to which the glycans are bound. ...
  http://www.patentsencyclopedia.com/app/20100297609
*  Plus it
Protein extracts were analyzed using anti-HRP antibody, which recognizes complex N-glycans with β-1,2-Xyl and core α-1,3-Fuc ... Protein Gel-Blot Analysis. Plant material was ground in liquid nitrogen using a mixer mill, resuspended in 10 µL of phosphate- ... but their involvement in the transfer of oligosaccharides to proteins has not been reported (Gallois et al., 1997; Danon et al ... from the lipid carrier dolichol pyrophosphate to selected Asn residues in the sequence Asn-X-Ser/Thr (where X ≠ P) within ...
  http://www.plantphysiol.org/content/162/1/24
*  Similarities and Differences in the Glycosylation Mechanisms in Prokaryotes and Eukaryotes
"Glycosylation of the phosphate binding protein, PstS, in Streptomyces coelicolor by a pathway that resembles protein O- ... "Translocation of lipid-linked oligosaccharides across the ER membrane requires Rft1 protein," Nature, vol. 415, no. 6870, pp. ... T. Endo, "O-Mannosyl glycans in mammals," Biochimica et Biophysica Acta, vol. 1473, no. 1, pp. 237-246, 1999. View at Publisher ... D. J. Kelleher, S. Banerjee, A. J. Cura, J. Samuelson, and R. Gilmore, "Dolichol-linked oligosaccharide selection by the ...
  https://www.hindawi.com/journals/ijmicro/2010/148178/ref/
*  US5354853A - Phospholipid-saccharide conjugates - Google Patents
Oligosaccharides typically contain from 2 to 10 monosaccharide units joined in glycosidic linkage. Polysaccharides (glycans) ... The biological role of dolichol.. Guo et al. 2001. Steric stabilization of fusogenic liposomes by a low-pH sensitive PEG− ... Preparation comprising hexose-6-phosphate-modified cholesterol derivative CN105153251B (en) * 2015-09-29. 2018-03-20. 浙江合糖科技有限公 ... protein or other determinant which is specific for a cell-surface receptor on the targeted cell type or tissue. For example, ...
  https://patents.google.com/patent/US5354853A/en
*  Carbohydrates and glycobiology: the '3rd alphabet of life' after DNA and proteins
Glycans - the third revolution in Molecular biology 1 The development and maintenance of a complex organism composed of ... one phosphate was originally linked to dolichol, and the second phosphate came from the nucleotide sugar). The oligosaccharide ... glycans) to generate the most complex epiproteomic modification - protein glycosylation. Very large number of different glycans ... The Third Alphabet of Life: Carbohydrate-Protein Interactions 1. The structural diversity of oligosaccharides found in ...
  http://reasonandscience.heavenforum.org/t2071-carbohydrates-and-glycobiology-the-3rd-alphabet-of-life-after-dna-and-proteins