*  Response of superficial proximal convoluted tubule to decreased and increased renal perfusion pressure. In vivo microperfusion...
Although urinary sodium excretion is positively influenced by acute changes in renal perfusion pressure, micropuncture studies show highly conflicting results concerning the response of superficial proximal tubule sodium reabsorption to changes in renal perfusion pressure. In the present study, the changes of superficial proximal reabsorption to decreased and increased renal perfusion pressure were determined in rats by an in vivo microperfusion method. In vivo microperfusion was selected as the method to determine the proximal sodium reabsorption because this method made it possible to deliver a constant fluid and electrolyte load to the proximal tubule without the influence of possible changes of glomerular filtration rate. Renal perfusion pressure was decreased from normal pressure by inflating a suprarenal aortic cuff and was increased from the normal level by the occlusion of celiac and mesenteric ...
  http://circres.ahajournals.org/content/66/5/1184
*  Plus it
Ferret (Mustela putorius furo, n = 45), pig (Yorkshire, n = 4), and sheep (Suffolk-Rambouillet, n = 5) tracheas were obtained ∼1 h postmortem (pentobarbital sodium injection) following experiments carried out for other purposes. No animals were euthanized specifically for these experiments; nevertheless, the experiments were approved by the Stanford University Institutional Animal Care and Use Committee. The mean age of the ferrets was 141.1 ± 23.7 days (n = 43); the youngest ferret was 59 days old and had abundant airway glands along its whole trachea. Pigs and sheep were adults. Human tracheas (n = 5) were obtained from surgical trim from lung transplantation donors, under procedures approved by Stanford's Institutional Review Board.. All tissues were transported to the laboratory in cold Physiosol solution (Abbott Laboratories) and transferred to ice-cold Krebs-Ringer bicarbonate buffer, bubbled with 95% O2-5% CO2, in which they were maintained until use, usually within 24 h. The ...
  http://ajplung.physiology.org/content/299/1/L124
*  GLP-1 Receptor Agonist Exenatide Increases Capillary Perfusion Independent of Nitric Oxide in Healthy Overweight...
In this mechanistic study, we demonstrate that the GLP-1RA exenatide increases capillary density, that is, microvascular perfusion, in healthy overweight men. These findings are consistent with several recent studies using native GLP-1 infusion in animals4,8,12,13 and humans,4,5 in which an increase in microvascular perfusion was demonstrated. We are the first to demonstrate that this vasoactive effect is also present with the use of a GLP-1RA in humans. Moreover, in contrast to previous findings using native GLP-1, we demonstrate that the microvascular effects of exenatide are independent of NO and insulin concentrations.. Capillary perfusion is regulated by precapillary arteriolar tone, which is modulated by several systemic and local factors. We demonstrate that exenatide increases perfusion, which can either be a direct or an indirect effect. A direct effect is conceivable, and can be explained by the observation that the GLP-1 receptor ...
  http://atvb.ahajournals.org/content/35/6/1538.long
*  Increase of Protein Yield in High Five Cells in a Single-Use Perfusion Bioreactor by Medium Replacement - Rausch - 2012 -...
Rausch, M., Pörtner, R. and Knäblein, J. (2013), Increase of Protein Yield in High Five Cells in a Single-Use Perfusion Bioreactor by Medium Replacement. Chemie Ingenieur Technik, 85: 111-117. doi: 10.1002/cite.201200121 ...
  http://onlinelibrary.wiley.com/doi/10.1002/cite.201200121/references?globalMessage=0
*  Investigation of cerebral perfusion changes following MDMA Ecstasy administration in an animal model using bolus-tracking...
The recreational drug of abuse 3,4 methylenedioxymethamphetamine (MDMA; Ecstasy) carries a risk of cerebrovascular accidents (CVA) that may relate to the role of serotonin (5- HT) and/or dopamine in the regulation of cerebrovascular tone. Recent advances in magnetic resonance imaging (MRI) have enabled measurement of cerebral blood perfusion using contrast agent-free approaches such as bolus-tracking arterial spin labeling (btASL). This investigation assessed changes in cerebral perfusion following systemic MDMA administration to rats using btASL MRI. Adult male Wistar rats were administered MDMA (5 or 20 mg/kg; i.p.) or saline, anaesthetised 1, 3 or 24 hours later and a high resolution anatomical scan followed by a continuous ASL (cASL) sequence was conducted using a 7 Tesla MRI scanner. Perfusion-weighted images were generated by subtraction of labelled from control images and experimental data was fitted to a quantitative model of cerebral ...
  http://www.tara.tcd.ie/handle/2262/78184
*  'edema cardiac' Protocols and Video...
Video articles in JoVE about edema cardiac include 'Quantitative Magnetic Resonance Imaging of Skeletal Muscle Disease', 'Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program', 'Assessment of Cardiac Function and Myocardial Morphology Using Small Animal Look-locker Inversion Recovery (SALLI) MRI in Rats', '2-Vessel Occlusion/Hypotension: A Rat Model of Global Brain Ischemia', 'Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging', 'In vivo Measurement of the Mouse Pulmonary Endothelial Surface Layer', 'Standardized Colon Ascendens Stent Peritonitis in Rats - a Simple, Feasible Animal Model to Induce Septic Acute Kidney Injury', 'Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow', 'Pericardiocentesis', 'Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery ...
  https://www.jove.com/keyword/edema+cardiac
*  'air pressure' Protocols and Video...
Video articles in JoVE about air pressure include 'Preparation of Carbon Nanosheets at Room Temperature', 'Rapid and Low-cost Prototyping of Medical Devices Using 3D Printed Molds for Liquid Injection Molding', 'Study of Siphon Breaker Experiment and Simulation for a Research Reactor', 'Whole-Body Nanoparticle Aerosol Inhalation Exposures', 'Conducting Miller-Urey Experiments', 'Quantitative Autonomic Testing', 'Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program', 'Biomass Conversion to Produce Hydrocarbon Liquid Fuel Via Hot-vapor Filtered Fast Pyrolysis and Catalytic Hydrotreating', 'Blast Quantification Using Hopkinson Pressure Bars', 'Surgical Placement of Catheters for Long-term Cardiovascular Exercise Testing in Swine', 'Laboratory Production of Biofuels and Biochemicals from a Rapeseed Oil through Catalytic Cracking Conversion', 'Ammonia Synthesis at Low Pressure', 'Design and Use of a Full Flow Sampling System (FFS) for the ...
  https://www.jove.com/keyword/air+pressure
*  Value of Gating of Technetium-99m Sestamibi Single-Photon Emission Computed Tomographic Imaging | JACC: Journal of the American...
This study, which was undertaken in a sequential group of heterogeneous patients referred to the Nuclear Cardiology Laboratory for testing, showed that the addition of gated SPECT acquisition for the assessment of regional systolic function reduces the degree of uncertainty in the interpretation of stress and rest Tc-99m sestamibi SPECT perfusion studies. The number of "borderline normal" and "borderline abnormal" interpretations when perfusion images were read without the gated images were significantly reduced when the gated tomograms were interpreted simultaneously with perfusion images, yielding better separation of normal from abnormal studies for coronary artery disease detection. It should be pointed out that when artifacts (e.g., breast attenuation, diaphragmatic attenuation) were unequivocally identified on perfusion images, those studies were read as normal or borderline normal. The "borderline abnormal" designation was used only ...
  http://www.onlinejacc.org/content/30/7/1687
*  Toronto XVIV0 Lung Perfusion System allows high-risk lungs to be safely transplanted
Results at 72 hours showed that 15% of the lungs treated with the Toronto XVIVO System had primary graft dysfunction, in comparison to 30% in the control group. Both groups of lung transplant patients had similar results in their secondary endpoints, i.e. 30-day mortality, bronchial complications, length of hospital and ICU stay and mechanical ventilation.. The Toronto XVIVO System was associated with gradual improvement in function in most lungs and, importantly, it was also helpful in determining a small subset of donor lungs (three) that were not suitable for transplantation. "For the first time, we can assess more precisely the function of an organ before using it. The most important finding of this study was that even donor lungs previously thought to be unusable can now be used for transplantation with excellent outcomes, if they perform with acceptable function on the XVIVO system. This will give us more lungs with more predictable, safer outcomes after transplantation, and shorter ...
  http://www.innovations-report.com/html/reports/medicine-health/toronto-xviv0-lung-perfusion-system-high-risk-lungs-173747.html
*  A new device for continuous assessment of gut perfusion: proof of concept on a porcine model of septic shock | Critical Care |...
The key findings of this experiment were a significant decrease of PAC and PDC during sepsis and a significant correlation with GM. PPG signal evolution was partially uncoupled with macrocirculatory parameters. A strong correlation between PAC, PDC and lactate concentration was found. Sepsis is characterized by a disruption in normal regulatory mechanisms that balance pro- and anti-inflammation and pro- and anti-coagulation. This imbalance results in cardiac and endothelial dysfunction, leading to macro- and microcirculation impairments. The GM is thought to be affected earlier than other territories [5], making its monitoring a promising means to improve treatment of sepsis. Improving gut perfusion has been associated with an improvement of intramucosal pH [23] and gastric mucosal tonometry, a surrogate of gastric mucosal perfusion [24], is one of the rare monitoring devices able to improve mortality of patients in septic shock [5]. In the aforementioned context, monitoring ...
  https://ccforum.biomedcentral.com/articles/10.1186/cc13992
*  Masimo to Present at 34th Annual J.P. Morgan Healthcare Conference | Masimo Corporation
NASDAQ: MASI) is a global leader in innovative noninvasive monitoring technologies. Our mission is to improve patient outcomes and reduce the cost of care by taking noninvasive monitoring to new sites and applications. In 1995, the company debuted Masimo SET® Measure-through Motion and Low Perfusion™ pulse oximetry, which has been shown in multiple studies to significantly reduce false alarms and accurately monitor for true alarms. The benefits of Masimo SET® have been proven in more than 100 independent and objective studies and it is estimated to be used on more than 100 million patients in leading hospitals and other healthcare settings around the world. In 2005 ...
  http://masimocorp.gcs-web.com/news-releases/news-release-details/masimo-present-34th-annual-jp-morgan-healthcare-conference
*  DLP® Coronary Perfusion Adapter, Y-Type
... ,This adapter features a female luer on the single leg. A rigid Y has two legs with white clamps and terminate with male luers.,medicine,medical supply,medical supplies,medical product
  http://www.bio-medicine.org/medicine-products/DLP-26reg-3B-Coronary-Perfusion-Adapter--Y-Type-17631-1/
*  Aortic plaque distribution in relation to cross-clamp and cannulation procedures during cardiac surgery
Background: Despite the successful development in cardiac surgery, cerebrovascular accidents (CVA) remain a devastating complication. Aortic atherosclerosis has been identified as a major risk factor for CVA. The present thesis addresses this question in relation to aortic manipulation during cardiac surgery, being divided into a clinical (I-II) and an experimental part (III-V).. Material and methods: Consecutive cardiac surgery cases (n=2641) were analyzed. Patients with CVA were extracted from a database designed to monitor clinical symptoms. Patient records were used to confirm clinical data and diagnosis. Subdivision was made into three groups: control subjects, immediate, and delayed CVA, being analyzed for neurological symptoms (I). Patients with CVA who also had been investigated with computer tomography (CT) (n=77) were further evaluated in terms of hemispheric and vascular distribution of lesions. The CT-findings were compared with CVA symptoms (II). An aortic perfusion model was ...
  http://umu.diva-portal.org/smash/record.jsf?pid=diva2:144051
*  High-frequency QRS electrocardiogram predicts perfusion defects during myocardial perfusion imaging.
BACKGROUND: Changes in high-frequency (HF) QRS components of the electrocardiogram (ECG) (150-250 Hz) are more sensitive than changes in conventional ST segments for detecting myocardial ischemia. We investigated the accuracy of 12-lead HF QRS ECG in
  http://www.biomedsearch.com/nih/High-frequency-QRS-electrocardiogram-predicts/16387056.html
*  Neurourology and Urodynamics - Volume 26, Issue 7 - November 2007 - Wiley Online Library
The extracorporeal perfusion of the female pig detrusor as an experimental model for the study of bladder contractility (pages 1024-1029). Ralf Dittrich, Matthias W. Beckmann, Caroline Maake, Patricia G. Oppelt, Andreas Mueller, Siegfried Mueller, Inge Hoffmann, Gert Naumann, Heinz Koelbl and Theodoros Maltaris. Version of Record online: 16 JUL 2007 , DOI: 10.1002/nau.20443. ...
  http://onlinelibrary.wiley.com/doi/10.1002/nau.v26:7/issuetoc
*  Isolation and culture of adult mouse hepatocytes - Opus
The liver performs a multitude of functions including the regulation of carbohydrate, fat, and protein metabolism, the detoxification of endo- and xenobiotics, and the synthesis and secretion of plasma proteins and bile. Isolated hepatocytes constitute a useful technique for studying liver function in an in vitro setting. Here we describe a method for the isolation of hepatocytes from adult mouse liver. The principle of the method is the two-step collagenase perfusion technique which involves sequential perfusion of the liver with ethylenediaminetetraacetic acid and collagenase. Following isolation, the cells can either be used for short-term studies or, alternatively, maintained in culture for prolonged periods to study long-term changes in gene expression. The protocol for mouse hepatocyte isolation may be applied to both normal and transgenic mice.. ...
  http://opus.bath.ac.uk/20342/
*  Severe sepsis/shock in the ED (1 month - 18 years) - Curbside
num":"0","calc_variableID":"","ev":"","ab":"","media":"","calc":""},{"trackID":"0","aID":"44822","blockId":"decisioncontainer63","nodetype":"decision","positionX":1091,"positionY":1933,"posW":110,"posH":110,"name":". Persistent poor perfusion at max dose?. ","orderbody":"","num":"0","calc_variableID":"","ev":"","ab":"","media":"","calc":""},{"trackID":"0","aID":"44823","blockId":"decisioncontainer61","nodetype":"decision","positionX":760,"positionY":2141,"posW":110,"posH":110,"name":". Persistent poor perfusion at max dose?. ","orderbody":"","num":"0","calc_variableID":"","ev":"","ab":"","media":"","calc":""},{"trackID":"0","aID":"44824","blockId":"notecontainer59","nodetype":"note","positionX":84,"positionY":1320,"posW":327,"posH":110,"name":". Do not delay antibotics for lumbar puncture. However, consider delaying lumbar puncture in patients that continues to be unstable, have severe respiratory compromise or signs of ...
  http://curbsideup.com/path.asp?pathID=806
*  Re: [Histonet] Perfusion debate
Strange we did not have shrinkage problems with our set up. Pam Marcum Original Message: ----------------- From: Charles W. Scouten, Ph.D. cwscouten@myneurolab.com Date: Wed, 8 Oct 2003 10:39:33 -0500 To: mucram11@earthlink.net, frouwke@sci.kun.nl, nancy.walker@sanofi-synthelabo.com, histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: shrinkage and Perfusion One system Yes, gravity will work, with 20% shrinkage of the brain, some shape distortion, and with a reddish tinge due to remaining red blood cells, which catalyze any HRP reaction, and autofluoresce. OK for some purposes, terrible for others, but not the optimum for any. I have asked www.Histonet.org to post a picture called Perfused Brains Annotated which shows whole rat brains, one fresh dissected out, one perfused by pressure sucrose and 4% paraformaldehyde/glut, and the last by traditional gravity flow with saline prewash, and the same fixative. The last is reddish and shrunken compared to the middle brain. Three ft of ...
  http://www.histosearch.com/histonet/Oct03/Re.HistonetPerfusiondebatB.html
*  Perfusion cannula definition | Drugs.com
Definition of perfusion cannula. Provided by Stedman's medical dictionary and Drugs.com. Includes medical terms and definitions.
  https://www.drugs.com/dict/perfusion-cannula.html
*  Intrathecal chemotherapy: brain tissue profiles after ventriculocisternal perfusion.
Abstract Ventriculocisternal perfusions with five isotopically labeled drugs were performed in the rhesus monkey and the resultant tissue diffusion concentration profiles in caudat..
  https://www.omicsonline.org/references/intrathecal-chemotherapy-brain-tissue-profiles-after-ventriculocisternal-perfusion-1441528.html
*  Perfusion Services
Procirca integrates staffing and personnel management, equipment and disposable management, quality assurance and improvement, compliance concerns, and program design into a cost-effective and high-quality perfusion program.
  http://www.procirca.com/perfusion-services/Pages/default.aspx
*  Woodworking (50 topics)
I got my first chance to use a up-cutting spiral bit yesterday with my router and I have mixed reviews about it.. First, the situation. I needed to cut mortises in some posts. Eight mortises in all, to be exact. The mortise dimensions were 3/4″ wide by 3 7/8″ long by 1 3/8″ deep. So long and somewhat skinny. The bit I have is 1/2″ in diameter.. The router I used is a Porter Cable 691 with the plunge router base. Because of the depth of the cut, I knew I'd need at least a couple passes to finish the cut. I was hoping that was all I'd need.. The good part is that the bit cuts very easily and cleanly, especially when compared to a straight cutting bit. The plunge into the wood was not a problem and, as long as I didn't attempt to remove too much material in a single pass, there was no bucking or chattering as I moved the bit through the wood. The bit even manages to eject some of the wood shavings, as I'd expect from an "up-cutter.". The bad is that it's not nearly as effective as I'd hoped ...
  http://sahd.lamafam.org/?cat=9
*  Anti Pig CD163 Antibody, clone 2A10/11 | Bio-Rad Antibodies (formerly AbD Serotec)
Mouse anti Pig CD163 antibody, clone 2A10/11 recognises porcine CD163, a 120 kDa single pass type 1 transmembrane cell surface glycoprotei
  https://www.bio-rad-antibodies.com/pig-porcine-cd163-antibody-2a10-11-mca2311ga.html
*  Neuropsychopharmacology - Residual Effects of THC via Novel Measures of Brain Perfusion and Metabolism in a Large Group of...
Neuropsychopharmacology, the official publication of the American College of Neuropsychopharmacology, publishing the highest quality original research and advancing our understanding of the brain and behavior.
  http://www.nature.com/npp/journal/vaop/ncurrent/full/npp201744a.html?foxtrotcallback=true&error=cookies_not_supported&code=273931a0-af54-40a6-8360-584ce0f80d28