Motion Analysis of Live Objects by Super-Resolution Fluorescence Microscopy
Establishing correspondence between particles in a sequence of frames with high particle density, particles merging and ... frictional coupling between the two membrane leaflets and between membrane and solid support . A simulation and estimation ... Researchers are exerting efforts not only in simple localization, but also in improving the future methods in other aspects.(1) ... A tracking algorithm is tested in  on solid-lipid nanoparticles diffusing within cells and on lymphocytes diffusing in ...https://www.hindawi.com/journals/cmmm/2012/859398/
UGA researchers boost efficacy of drugs by using nanoparticles to target 'powerhouse... ( Athens Ga. - Nanoparticles have show...
... researchers,boost,efficacy,of,drugs,by,using,nanoparticles,to,target,'powerhouse,of,cells',medicine,medical news today,latest ... Nanoparticles have shown great promise in the targeted d...By targeting mitochondria often called the powerhouse of cells the ... Upon entering cells, nanoparticles enter a sorting center known as the endosome. The first thing Dhar and Marrache had to ... The outer membrane only permits molecules of a certain size to pass through, while the inner membrane only permits molecules of ...http://www.bio-medicine.org/medicine-news-1/UGA-researchers-boost-efficacy-of-drugs-by-using-nanoparticles-to-target-powerhouse-of-cells-94612-1/
Hemagglutinin (influenza) - Wikipedia
... and pulls the endosomal membrane right up next to the virus particle's own membrane, causing the two to fuse together. Once ... It is also responsible for the fusion of the viral envelope with the endosome membrane, after the pH has been reduced. The name ... the opening of which is thought to be the first step in the membrane fusion process. In 2015 researchers designed an immunogen ... One team designed self-assembling HA-stem nanoparticles, using a protein called ferritin to hold the HA together. Another ...https://en.wikipedia.org/wiki/Hemagglutinin_(influenza)
Frontiers | Nanoparticle-Based and Bioengineered Probes and Sensors to Detect Physiological and Pathological Biomarkers in...
In this context, quantum dots (QDs), carbon-based structures (C-dots, graphene and nanodiamonds) and gold nanoparticles are the ... In this context, quantum dots (QDs), carbon-based structures (C-dots, graphene and nanodiamonds) and gold nanoparticles are the ... suggest that the particle quantity, NP surface properties and membrane structures are closely linked with NP-membrane forces ... Endocytosis and cooperative transmembrane penetration of nanoparticles are the ways by which nanoparticles can enter cells ( ...https://www.frontiersin.org/articles/10.3389/fnins.2015.00480/full
Nanotechnology Now - Press Release: Researchers create new 'smart' nanocapsule delivery system for use in protein therapy
"Here, we've been able to use this new technology to stabilize the protein, making it very easy to cross the cell membrane, ... When the protein nanocapsule is taken in by the cell, it will stay within the endosome initially. Endosomes generally have ... Leti Develops World s First Micro-Coolers for CERN Particle Detectors: Leti Design, Fabrication and Packaging Expertise Extends ... Drug-delivering nanoparticles seek and destroy elusive cancer stem cells November 28th, 2017 ...http://www.nanotech-now.com/news.cgi?story_id=35917
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Nanoparticles, Endosome Escape, Intracellular Trafficking, Cytosolic Drug Delivery, red blood cells, assay ... In this model system the erythrocyte membrane serves as a surrogate for the lipid bilayer membrane that enclose endo-lysosomal ... As such, researchers have more recently used microfluidic systems to study blood cell deformability, blood cell aggregation, ... particle size distribution, and proximate and ultimate analysis. Also described in the protocol are the analyses of the ...https://www.jove.com/visualize/abstract/20090940/hypoxia-activates-ca2-permeable-cation-conductance-sensitive-to
Protocols and Video Articles Authored by Shuming Nie
As a result, researchers can tune the light emission from these particles throughout the ultraviolet, visible, near-infrared, ... 2005 , Pubmed ID: 17282550 Nanometer-sized particles such as luminescent quantum dots (QDs) and energy-transfer nanoparticles ... The proton-resistant QDs are found to cause moderate membrane damage to cultured epithelial cells, but PEGylation (PEG grafting ... Cell-penetrating Quantum Dots Based on Multivalent and Endosome-disrupting Surface Coatings Journal of the American Chemical ...https://www.jove.com/author/Shuming_Nie
Nanomaterials in medicine and pharmaceuticals: nanoscale materials developed with less toxicity and more efficacy : European...
The most interesting thing is that these nanoparticles can escape from the endosome and release the payloads effectively in ... prepared rod-like nano-HAp/gelatin scaffolds coated with nano-HAp particles. The HAp particles on the scaffold wall easily ... Researchers have compared the utility of USPIOs with Gd-chelates for brain-tumor MRI and found that USPIOs could offer ... The increasing drug efflux is caused by the integral membrane proteins, known as MDR transporters. The transporters such as p- ...https://www.degruyter.com/view/j/ejnm.2013.5.issue-2/ejnm-2013-0003/ejnm-2013-0003.xml
Pharmaceutics | Free Full-Text | Exploring the Correlation Between Lipid Packaging in Lipoplexes and Their Transfection...
Some other researchers add that packing properties of the lipids used in the formulation of lipoplexes may also play a role in ... allowing the release of the DNA from the endosome and the lipoplex, leaving it free it to enter the nucleus [11,31]. However in ... Alternatives to cholesterol as a membrane stabilising agent. J. Pharm. Pharmacol. 2010, 62, 1646-1655. [Google Scholar] ... causing aggregation of large particles to form with lower transfection efficacy presumably due to reduced internalisation . ...http://www.mdpi.com/1999-4923/3/4/848/htm
Manash (Subhaditya Edusoft): Subhaditya News Channel Presents Science,Movie,Political ,Sports and Book News This Two Weeks (128...
... creating an excess of these charged particles just outside its inner membrane. TH enzymes, which are fixed at one end within ... In order for the protein to be effective once inside the cell, it must be released from the vesicle (endosome) holding it. The ... The researchers exploited the receptors on prostate cancer cells, which rely on the recognition of a C-end rule internalizing ... The team used a modular nickel linking layer on the surface of the nanoparticles that is able to support different kinds of ...http://manashsubhaditya.blogspot.com/2015/01/subhaditya-news-channel-presents.html
Field responsive materials: photo-, electro-, magnetic- and ultrasound-sensitive polymers - Polymer Chemistry (RSC Publishing)...
The drug loaded nanoparticles were taken up by the cells via the endocytosis pathway and reached the late endosome, where they ... d) Proposed mechanism for the internalization of the nanoparticles into the HeLa cells followed by particle photo-chemo-lysis ... Maximum displacements were achieved when the field was applied at the periphery of the membrane, causing its buckling.160 ... researchers extended the synthetic route to the synthesis of photo-responsive membranes with light controlled permeability and ...http://pubs.rsc.org/en/content/articlehtml/2016/py/c6py01455k
IJMS | Free Full-Text | Bispecific Antibodies as a Development Platform for New Concepts and Treatment Strategies | HTML
... end and formulated into nanoparticles consisting of dynamic polyconjugates (DPCs) or into lipid-based nanoparticles (LNPs). ... A single-chain antibody fragment against DOTA complexed with β particle-emitting radio metals, such as 177Lu and 90Y, was ... The CH3 domain is a potential site for researchers to modify and add some entities to create multifunctional molecules. ... Finally, minicells loaded with chemotherapeutic drugs were targeted via bispecific antibodies to antigens on the membrane of ...http://www.mdpi.com/1422-0067/18/1/48/htm
Jianghong Rao | Stanford Medicine Profiles
Development of novel tumor-targeted theranostic nanoparticles activated by membrane-type matrix metalloproteinases for combined ... Small particles are in part renally excreted. Peptide-coated particles are cleared from liver faster than physical decay alone ... report here novel molecular imaging probes that specifically target GTP-bound Rab5 associated with the early endosome membrane ... Semiconductor quantum dots (QDs) have captivated researchers in the biomedical field over the last decade. Compared to organic ...https://med.stanford.edu/profiles/jianghong-rao
Systematic review of factors influencing extracellular vesicle yield from cell cultures
2014). In TRPS, conducting medium containing nanoparticles passes through a nano-sized pore in a non-conductive membrane. The ... such as differentiation of particles by their refractive index. Researchers working with EVs derived from cell cultures need to ... One of the main subpopulations of EVs is the endosome-derived exosome (Akers et al. 2013). Exosomes are formed by inward ... Particle yields were not included due to the fact that 25 of the 34 particle yield counts were obtained with UC and UC-W. Less ...http://pubmedcentralcanada.ca/pmcc/articles/PMC4960200/
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Nanoparticles, Endosome Escape, Intracellular Trafficking, Cytosolic Drug Delivery, red blood cells, assay ... Membrane trafficking involves transport of proteins from the plasma membrane to the cell interior (i.e. endocytosis) followed ... Overall, our method provides a straightforward way for researchers to rapidly gain further insight into protein-ligand ... and phagocytic particles. ... Plasma membrane injury is a frequent event, and wounds have to ...https://www.jove.com/visualize/abstract/21887293/loss-niemann-pick-c1-or-c2-protein-results-similar-biochemical
Nanomaterials | Free Full-Text | Autophagy as a Possible Underlying Mechanism of Nanomaterial Toxicity | HTML
... outer membrane mitochondria , Golgi  or plasma membrane . The initiation step of autophagy involves two major ... Chen, H.W.; Su, S.F.; Chien, C.T.; Lin, W.H.; Yu, S.L.; Chou, C.C.; Chen, J.J.; Yang, P.C. Titanium dioxide nanoparticles ... Indeed, researchers used acidic NP to lower the pH of compromised lysosomes in human retinal pigment epithelial cells and thus ... It is interesting to note that, before the autophagosome/lysosome fusion, the autophagosome may also fuse with an endosome to ...http://www.mdpi.com/2079-4991/4/3/548/htm
PVM/MA-shelled selol nanocapsules promote cell cycle arrest in A549 lung adenocarcinoma cells | Journal of Nanobiotechnology |...
In d, the arrow points to an endosome. In e and f, the ultrastructural morphology is unchanged by SPN treatment. N, M, ER, GC, ... Therefore, some researchers have aimed to find organic selenium compounds with higher therapeutic indexes [23,24]. Selol, an ... Bl nanoparticles were prepared using the same method with the same concentrations of the components used for SPN but without ... Mitochondrial membrane potential and by DNA fragmentation of A549 cells treated with selol-PVM/MA nanocapsules (SPN) and ...https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-014-0032-x
Guofeng Zhang | National Institute of Biomedical Imaging and Bioengineering
Mechanical tension drives cell membrane fusion. Kim JH, Ren Y, Ng WP, Li S, Son S, Kee YS, Zhang S, Zhang G, Fletcher DA, ... Monoubiquitination of EEA1 regulates endosome fusion and trafficking. Ramanathan HN, Zhang G, Ye Y. Cell Biosci. 2013 May 23. ... Polyaspartic acid coated manganese oxide nanoparticles for efficient liver MRI. Xing R, Zhang F, Xie J, Aronova M, Zhang G, Guo ... A new peptide ligand that targets particles and heterologous proteins to hepatocytes in vivo. Sokoloff AV, Wong SC, Ludtke JJ, ...https://www.nibib.nih.gov/about-nibib/staff/guofeng-zhang
Photo: Capture of a vesicle by an endosome by the tethering factor EEA1 binding Rab5. Active Rab5 (shiny blue particles) ... Photo: In a series of meetings got together the researchers of the FANTOM5 consortium in that took part over 250 researchers in ... The membrane itself must be recognized by a target membrane, which employs long tethering proteins to find its match. ... to create a nanometer-sized photonic circuit with the helices of the genetic code carrier and gold nanoparticles.. In the ...http://www.biotec.tu-dresden.de/news.html
Site-specific targeting of enterovirus capsid by functionalized monodisperse gold nanoclusters | PNAS
... one may evaluate the spatial location of the virus in relation to the receptor or the limiting membrane of the endosome. In ... On one hand, the particles of PTA salt are able to enter the grooves of the virus capsid in C. On the other hand, only the ... Researchers report an early monumental burial site near Lake Turkana in Kenya that may have served as a stable landmark for ... Linking colloidal gold nanoparticles and phosphine-functionalized gold nanoclusters to biomolecules has been a topic of wide ...http://www.pnas.org/content/111/4/1277
Protocols and Video Articles Authored by Wei Tian
The optimal membrane can be chosen based on the patient and tissue type to realize the synchronization of membrane degradation ... As a neighbor of carbon in the periodic table, element boron is interesting and many researchers have contributed their efforts ... Hydrodynamic diameter and zeta potential analysis demonstrated the formation of GNS/siHSP72/HA having a particle size of 73.2 ... Aug, 2015 , Pubmed ID: 26238737 A composite photoanode comprising ultralong ZnO nanobelts and TiO2 nanoparticles was prepared ...https://www.jove.com/author/Wei_Tian
Jamboree/Project Abstract/Team Abstracts - 2010.igem.org
Bacterial cells are lysed in the endosome and then LLO is released. LLO is a pore-forming toxin which causes endosomal membrane ... Enabling researchers to synthesize their own enzymes and having access to inexpensive tools will allow for increased ... Mms6 from Magnetospirillum magneticum removes heavy metals from solution by forming nanoparticles. The Mms6 protein will be ... enzymes as well as readily assembled vectors for gene delivery and production of non-replicative virus particles. The viral ...http://2010.igem.org/wiki/index.php?title=Jamboree/Project_Abstract/Team_Abstracts&diff=208669&oldid=78270
Protocols and Video Articles Authored by Min Zhu
Expression of Platelet Membrane Glycoprotein Ib/IX/V Complex, a Receptor of Thrombin, in Patients with Hemorrhagic Thrombopathy ... The fusion of endosome and lysosome in epithelial cells was observed by immunocytochemistry. The antigenicity of protein ... Inactivation of spaP eliminated the small-particle DDAG. The results suggest that IrvA promotes DDAG not only by GbpC, but also ... Oxidative Damage of Lung and Its Protective Mechanism in Mice Caused by Long-term Exposure to Titanium Dioxide Nanoparticles ...https://www.jove.com/author/Min_Zhu
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
The endocytosed PNs could transport to apical early endosome (AEE) and then from AEE to lysososmes via AEE/late endosome (LE)/ ... The transport mechanisms of polymer nanoparticles in Caco-2 epithelial cells.. Bing He, Ping Lin, Zengrong Jia, Wenwen Du, Wei ... Both ER/Golgi and Golgi/REC/plasma membrane (PM) pathways were involved in the exocytosis of PNs. The transcytosis of PNs ... Cartilage regeneration after trauma is still a great challenge for clinicians and researchers due to many reasons, such as ...https://www.jove.com/visualize?author=Yuan-Long+Lan
Go to Publications.
Identification of the B-cell epitopes of the early endosome antigen 1 (EEA1).. Selak S1, Mahler M, Miyachi K, Fritzler ML, ... Effects of copy center particles on the lungs: a toxicological characterization using a Balb/c mouse model.. Pirela S1, Molina ... Eve Technologies offers the most affordable assay services in the world for biomedical and life science researchers, and ... Disease / Biomarker Category: Immunology, Nanoparticles, Drug Delivery. Synthesis and characterization of novel zwitterionic ...https://www.evetechnologies.com/publications/