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*  Blog on Optical Tweezers: November 2011
... which we experimentally measure here. This experimental constraint-combined with other experimentally determined parameters-is ... Deletion studies revealed that period 3 is important for the positive cooperative effect of Tm on actin filament regulation and ... Mechanical stochastic tug-of-war models cannot explain bidirectional lipid-droplet transport ... We used optical tweezers to rotate bacterial cells relative to the optical axis. We rapidly oscillate the optical tweezers ...
  http://opticaltweezers.blogspot.com/2011_11_01_archive.html
*  Difference between revisions of "User:Pranav Rathi/Notebook/OT/2012/11/13/Optical Tweezers Calibration" - OpenWetWare
To perform accurate measurements with optical tweezers, it is necessary to calibrate some experimental parameters and even ... So it is second most important component of the tweezers. AOM has two components: AOM driver and AOM module, I characterized ... I put a water droplet on the objective and record the power with a power meter directly. I used Thorlabs sensor: model D10MM ( ... It is absolutely unpractical to measure the laser power in the trap before every power-spectrum data is acquired to calculate ...
  https://openwetware.org/wiki/?title=User:Pranav_Rathi/Notebook/OT/2012/11/13/Optical_Tweezers_Calibration&curid=126877&diff=657417&oldid=657414
*  User:Pranav Rathi/Notebook/OT/2012/11/13/Optical Tweezers Calibration - OpenWetWare
To perform accurate measurements with optical tweezers, it is necessary to calibrate some experimental parameters and even ... It is important to know some details about the source. IR-laser is more important because it is tweezing laser. I did not do a ... I put a water droplet on the objective and record the power with a power meter directly. I used Thorlabs sensor: model D10MM ( ... It is absolutely unpractical to measure the laser power in the trap before every power-spectrum data is acquired to calculate ...
  https://openwetware.org/wiki/?title=User:Pranav_Rathi/Notebook/OT/2012/11/13/Optical_Tweezers_Calibration&curid=126877&oldid=657654
*  Open access articles from Soft Matter Home
Using Brownian motion to measure shape asymmetry in mesoscopic matter using optical tweezers Basudev Roy, Argha Mondal, Sudipta ... The bending modulus κ is known to be a crucial parameter for the stability of the droplet phase. By combination of droplet ... The interaction of protein-coated bionanoparticles and surface receptors reevaluated: how important is the number of bonds? ... Steady-state droplet size in montmorillonite stabilised emulsions William J. Ganley and Jeroen S. van Duijneveldt ...
  http://pubs.rsc.org/en/journals/articlecollectionlanding?sercode=sm&themeid=e297b895-0853-410c-8079-aa5917ba7475
*  Difference between revisions of "User:Pranav Rathi/Notebook/OT/2012/11/13/Optical Tweezers Calibration" - OpenWetWare
Optical tweezers calibration schematics in image shows all the parameters and programs I use. To determine the force exerted by ... It is important to know some details about the source. IR-laser is more important because it is tweezing laser. I did not do a ... I put a water droplet on the objective and record the power with a power meter directly. I used Thorlabs sensor: model D10MM ( ... It is absolutely unpractical to measure the laser power in the trap before every power-spectrum data is acquired to calculate ...
  https://openwetware.org/wiki/?title=User:Pranav_Rathi/Notebook/OT/2012/11/13/Optical_Tweezers_Calibration&diff=658487&oldid=658472
*  Laser manipulation in liquid crystals: an approach to microfluidics and micromachines | Philosophical Transactions of the Royal...
You described the use of optical tweezers to drag a colloidal particle through a nematic liquid and measure from the velocity ... We did measure the relative intensities of the π and σ polarizations after passing through droplets and we noted that droplet ... A convenient parameter to distinguish the regimes is the Ericksen number Er, defined by , where v∞ and r are characteristic ... In the case of LC droplets, viscoelasticity rather than isotropic viscosity is likely to be important, but it is nonetheless ...
  http://rsta.royalsocietypublishing.org/content/364/1847/2789
*  Live cell imaging of mechanotransduction | Journal of The Royal Society Interface
Relatively local stretch can be conveniently applied on cells with laser/optical tweezers or magnetic tweezers. Optical ... 2006 A dual parameter FRET probe for measuring PKC and PKA activity in living cells. J. Am. Chem. Soc. 128, 24-25. (doi:10.1021 ... Calcium ion is one of the most important biological signals (Berridge et al. 2000; Kim et al. 2009). It has been proved to play ... When the tip of the force sensor is inserted into the droplet of FN solution to suck the liquid and result in stretching the FN ...
  http://rsif.royalsocietypublishing.org/content/early/2010/03/29/rsif.2010.0042.focus
*  ACPD - Recent
One of the most important parameters in climate science is the equilibrium climate sensitivity (ECS). Estimates of this ... index of insoluble organic extracts from atmospheric aerosol over the visible wavelength range using optical tweezers Rosalie H ... The role of droplet sedimentation in the evolution of low level clouds over Southern West Africa Christopher Dearden, Adrian ... We collected a 15 m ice core from the Canadian High Arctic to measure a 38-year deposition record of PFAAs, proving information ...
  https://www.atmos-chem-phys-discuss.net/
*  Publications | Stavans Lab
By scattering a focused laser beam from a particle observed in an optical microscope, we measure its fluctuations via the ... Phase diagrams of droplet volume fraction versus polymer concentration are presented. At large droplet volume fractions where ... HU plays important roles as an accessory architectural factor in a variety of bacterial cellular processes such as DNA ... The model exhibits a one-parameter family of fixed-point distributions x(l)*(sigma). Within this model, universality is ...
  http://www.weizmann.ac.il/complex/stavans/publications
*  Analyzing Large Protein Complexes by Structural Mass Spectrometry | Protocol
It is also important to examine the nanoflow capillary prior to initiation of spray. The droplet size is a function of the tip ... Place the capillary under an optical microscope and trim the tip, using the sharp AA-type tweezers. ... Optimize these parameters, in combination with the nanoflow pressure.. *To improve desolvation and strip off residual water and ... Moreover, the technique can simultaneously measure the characteristics of separate populations co-existing in a mixture. ...
  https://www.jove.com/video/1954/analyzing-large-protein-complexes-by-structural-mass-spectrometry
*  Download Alien Encounters
Prerequisite of practice; transition tweezers findings in new military reflection projects. download of application; optical ... not makes an able, how to sense on how I had the download alien encounters and developed a important use of Mac OS X. batteries ... now 13 queue in link( more on the droplet). lost from and been by Amazon. Please call a sexual UK gene. Please become a ... The download alien that it is consistent to not like imaging TV critical ce( articulating fMRI and EEG) to measure whether ...
  http://pd19.org/ebook/download-alien-encounters
*  New England Complex Fluids Workgroup
... which is a direct measure of the viscoelastic response of the material.. Keywords: Microrheology, Biopolymer, Optical tweezers ... We have developed a droplet-based microfluidics technology that is capable of first encapsulating single cells in droplet, ... quantitatively measure kinetic parameters of the reversible crosslinks, including reaction rates and activation energies, from ... Pickering emulsions are important in systems where controlled confinement of an oil or water phase is needed. For example, ...
  http://www.complexfluids.org/necf/abstracts.php?meetingID=75
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... optical tweezers, flow stretching, and magnetic tweezers. Amongst these approaches, magnetic tweezers have distinguished ... magnetic tweezers and the freely-orbiting magnetic tweezers, which makes it possible to directly measure the torque stored in a ... It is also important to consider that since most cognitive and locomotor impairments strike in the aged individual, determining ... Parameters addressed with these protocols yield the minimum on information essential to characterize and understand the ...
  https://www.jove.com/visualize/abstract/19956649/systematic-differences-in-impact-across-publication-tracks-at-pnas
*  Blog on Optical Tweezers: January 2010
The conventional quadrant detection scheme is applied ubiquitously in optical tweezers experiments to quantify these parameters ... this being particularly important for deformations of cells. This deformation effect of electromagnetic radiation is important ... If the object is relatively easy to deform, as is, e.g., a low surface tension droplet, the resulting deformation is easily ... combination of opposing optical and fluid drag forces on colloidal samples within microfluidic environments to both measure ...
  http://opticaltweezers.blogspot.com/2010/01/
*  Blog on Optical Tweezers: May 2012
We measure the fluctuations of the position of a Brownian particle confined by an optical trap in an aging gelatin droplet ... of further increasing the measurement rates of the optical stretcher in parameter domains where hydrodynamic and optical forces ... with important consequences for microscopy, optical sensing and control of light by light at the nanoscale. DOI ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (4) Optical Memory and Neural Networks (1) Optical ...
  http://opticaltweezers.blogspot.com/2012/05/
*  Microfluidic tools for cell biological research
... optical tweezers [116], or complex dedicated force machines [117]. Most of these apparatus measure global and local forces of ... Picture of a cell trapping array using hydrodynamic trap and zoom on a single cell trap [168]. (B) Droplet based yeast culture ... Medium renewal is a key parameter for long-term cell experiment. Traditional usage of a sealed glass slide is convenient but ... Those researchers developed important technological advancements (e.g., multilayer molds or 3D microfluidic structures), ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2998071/
*  Blog on Optical Tweezers: February 2010
We used optical tweezers to measure the rupture force between HU proteins and Lon; HUα, HUβ, and HUβ-A20D can bind to Lon, and ... Side imaging was used in conjunction with imaging in the plane of the optical trap. Droplet sizing was performed using cavity- ... not only can produce a bright spot but also can form an optical cage in the focal region by changing the truncation parameter β ... Positioning technology is one of the most important technologies for developingmicrosystems. In particular, displacement ...
  http://opticaltweezers.blogspot.com/2010/02/
*  Blog on Optical Tweezers: September 2010
... and show that the charge of counterions is an important control parameter. These measurements show that the mobility of ... Droplet manipulation and trapping potential in such a two-dimensional anisotropic system show that optical trapping has two ... By combining experimental data with a physical model and an optimization algorithm for analysis, we measure the 10 unique ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (3) Optical Memory and Neural Networks (1) Optical ...
  http://opticaltweezers.blogspot.com/2010_09_01_archive.html
*  Blog on Optical Tweezers: September 2009
We investigate the mechanics and the dynamics of RBCs by a unique noninvasive technique, using weak optical tweezers to measure ... we produced cytoplasmic protrusions with optical tweezers in mature BY-2 suspension cultured cells to study the parameters ... It also facilitates the use of an isolated droplet of known composition as a sensitive probe of the gas phase conditions in an ... Microbial capsules are important for virulence, but their architecture and physical properties are poorly understood. The human ...
  http://opticaltweezers.blogspot.com/2009_09_01_archive.html
*  Blog on Optical Tweezers: January 2011
... so it is particularly important to understand the mechanics of its membrane attachment. We used optical tweezers to measure the ... a nematic droplet held in laser tweezers. The identification and spatial evolution of such a topological fingerprint is ... By analyzing the printing mechanism, we identify the key physical parameters controlling the method, which has the potential ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (3) Optical Memory and Neural Networks (1) Optical ...
  http://opticaltweezers.blogspot.com/2011_01_01_archive.html
*  Blog on Optical Tweezers: January 2013
Beyond its potential to provide control over the reaction coordinate, force is also an important physiological parameter that ... In recent years, optical tweezers have become one of the tools to measure the mechanical properties of living cells. In this ... the ability of the droplet to uptake water from the air depends on the solute weight percent of sodium chloride present in a ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (4) Optical Memory and Neural Networks (1) Optical ...
  http://opticaltweezers.blogspot.com/2013/01/
*  Patente US20110229872 - Microfabricated Cell Sorter - Google Patentes
... can be sorted into an appropriate branch channel based on the presence or amount of a detectable signal such as an optical ... Optical Trapping. * [0175] In another embodiment, an optical trap, or laser tweezers, may be used to sort or direct cells in a ... A charged droplet may cause the next droplet of the opposite polarity to have a reduced charge. On the other hand, if ... The parameters which govern this relationship are the volume of the detection region and the concentration of cells in the ...
  http://www.google.es/patents/US20110229872
*  Blog on Optical Tweezers: November 2009
... we measure with high accuracy both the optical trapping parameters and the rotation frequency induced by the radiation pressure ... The optical properties of the droplet are determined using stimulated Raman scattering. The droplet is in thermodynamic ... both of which are important in controlling the balance of endocytic sorting. To the best of our knowledge, this is a unique ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (3) Optical Memory and Neural Networks (1) Optical ...
  http://opticaltweezers.blogspot.com/2009_11_01_archive.html
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Nanomanipulation of Single RNA Molecules by Optical Tweezers. Authors: William Stephenson, Gorby Wan, Scott A. Tenenbaum, Pan T ... Carbon dioxide (CO2) along with oxygen (O2) share the role of being the most important gases in the human body. The measuring ... Advantages of the ultra-thin layer method over other sample deposition approaches (e.g. dried droplet) are that it provides (i ... Shape changes in the discriminant function analysis are based on a statistical measure, and therefore can be evaluated by a p- ...
  https://www.jove.com/visualize/abstract/23516637/analysis-laboratory-repeat-critical-values-at-large-tertiary-teaching
*  Blog on Optical Tweezers: June 2010
... an effective method for optical manipulation that overcomes the important obstacles such as small optical trapping forces, ... manipulated with an optical trap, and a streptavidin-functionalized coverslip were used to measure the effect of different ... Here, the nanoparticles covered the entire area of the assembled SnO2 rod by evaporating a droplet of nanoparticle solution ... By integrating into the classical theory a complex-valued channel-compliance parameter-that modulates the phase and amplitude ...
  http://opticaltweezers.blogspot.com/2010_06_01_archive.html