Involvement of the Src-cortactin pathway in podosome formation and turnover during polarization of cultured osteoclasts |...
6B) in untreated osteoclasts, as well as in osteoclasts overexpressing the different Src mutants. Osteoclasts expressed high ... Osteoclasts are large, multinucleated cells that adhere to bone via podosomes, and degrade it. During osteoclast polarization, ... RAW-derived osteoclasts were co-transfected with GFP-actin and corWT-flag or GFP-actin and cor3YF-flag. Cells were fixed and ... A) Control RAW osteoclasts, (B) cells co-expressing GFP-actin and SrcY527F, or (C) cells co-expressing GFP-actin and Src251 ...http://jcs.biologists.org/content/119/23/4878
Gambogic acid inhibits osteoclast formation and ovariectomy-induced osteoporosis by suppressing the JNK, p38 and AKT signaling...
Taken together, our results demonstrate that GBA inhibits osteoclast formation in vitro and in vivo , suggesting that it is of ... Gambogic acid inhibits osteoclast formation and ovariectomy-induced osteoporosis by suppressing the JNK, p38 and AKT signaling ... Excessive osteoclast formation and bone resorption are key causes of osteoporosis. Natural compounds can serve as alternative ... Gambogic acid inhibits osteoclast formation and ovariectomy-induced osteoporosis by suppressing the JNK, p38 and AKT signaling ...http://www.biochemj.org/content/early/2015/06/10/BJ20150151
"Active caspase-3 is required for osteoclast differentiation" by K. H. Szymczyk, T. A. Freeman et al.
The Proceedings of the 8th ICCBMT). We noted that osteoclast numbers are decreased in long bones of procaspase-3 knockout mice ... Together these findings provide direct support for the hypothesis that caspase-3 activity is required for osteoclast ... we investigated the role of this protein in the differentiation of primary osteoclasts and RAW264.7 cells (Szymczyk KH, et al, ... 2005, Caspase-3 activity is necessary for RANKL-induced osteoclast differentiation. ...https://digitalcommons.pcom.edu/scholarly_papers/1588/
Atp6v1c1 is an essential component of the osteoclast proton pump and in F-actin ring formation in osteoclasts | Biochemical...
Atp6v1c1 is an essential component of the osteoclast proton pump and in F-actin ring formation in osteoclasts. Shengmei Feng, ... The present study demonstrates that Atp6v1c1 is an essential component of the osteoclast proton pump at the osteoclast ruffled ... Atp6v1c1 is an essential component of the osteoclast proton pump and in F-actin ring formation in osteoclasts ... Atp6v1c1 is an essential component of the osteoclast proton pump and in F-actin ring formation in osteoclasts ...http://www.biochemj.org/content/417/1/195
Hyaluronan inhibits osteoclast differentiation via Toll-like receptor 4 | Journal of Cell Science
BMMs differentiate to osteoclasts in the presence of M-CSF and RANKL, whereas RAW264.7 cells form osteoclasts in the presence ... Osteoclast culture. To generate mouse bone marrow derived osteoclasts, monocytes were isolated from tibiae of 6-week-old ICR or ... TLR4 is expressed in both BMM osteoclast precursor cells and differentiated osteoclasts (Takami et al., 2002). HA binds TLR4 in ... Osteoclast differentiation factor acts as a multifunctional regulator in murine osteoclast differentiation and function. J. ...http://jcs.biologists.org/content/120/1/166
c-Fms and the alphavbeta3 integrin collaborate during osteoclast differentiation. - Semantic Scholar
Finally, like high-dose MCSF, overexpression of c-Fos normalizes the number of beta(3)(-/-) osteoclasts in vitro, but not their ... In vitro, however, the number of beta(3)(-/-) osteoclasts is reduced because of arrested differentiation. This paradox suggests ... correlating with the increase in the osteoclast number. To identify components of the MCSF receptor that is critical for ... osteoclastogenesis in beta(3)(-/-) cells, we retrovirally transduced authentic osteoclast precursors with chimeric c-Fms ...https://www.semanticscholar.org/paper/c-Fms-and-the-alphavbeta3-integrin-collaborate-dur-Faccio-Takeshita/ae344a69fa8034be3370969248ae835f20965d47
Role of the osteoclast in cranial suture waveform patterning - Byron - 2006 - The Anatomical Record - Wiley Online Library
1995) that osteoclasts are only found in the diploic spaces of flat bones and Vastardis et al. (2004) that osteoclasts are not ... These raw osteoclast counts reveal that bone resorption reaches its climax at 6 weeks of age. By 6 months, osteoclast activity ... 4) and osteoclast knockdown data (Fig. 8), it seems clear that osteoclast activity is involved in sagittal suture patterning. ... Osteoclasts along straight margins follow a very similar pattern except that the maximum number of straight osteoclasts is ...http://onlinelibrary.wiley.com/doi/10.1002/ar.a.20322/full
Kahweol | CAS#6894-43-5 | includes inhibition of RANKL-induced osteoclast generation | MedKoo
It exhibits a wide variety of biological activities, including inhibiting RANKL-induced osteoclast generation, inducing cell ... It exhibits a wide variety of biological activities, including inhibiting RANKL-induced osteoclast generation, inducing cell ...http://www.medkoo.com/products/15202
Interleukin-6 and interleukin-11 support human osteoclast formation by a RANKL-independent mechanism. - Nuffield Department of...
In order to determine whether IL-6 and IL-11 could independently support human osteoclast formation, these factors were added ... are directly capable of inducing osteoclast formation by a RANKL-independent mechanism. ... are known to influence osteoclast formation and bone resorption. ... are directly capable of inducing osteoclast formation by a ... Interleukin-6 and interleukin-11 support human osteoclast formation by a RANKL-independent mechanism. Share Share Share Share ...https://www.ndorms.ox.ac.uk/publications/103838
Transforming growth factor-beta induces osteoclast formation in the absence of RANKL. - Nuffield Department of Orthopaedics,...
TGFbeta-induced osteoclast formation was qualitatively different from that induced by RANKL with numerous TRAP+/VNR+ ... TGFbeta is known to regulate RANKL-induced osteoclast formation and bone resorbing activity. In this study we sought to ... directly induce mononuclear phagocyte osteoclast precursors to differentiate into osteoclastic cells capable of lacunar ... determine whether TGFbeta could directly induce osteoclast formation by a RANKL-independent mechanism. We found that the ...https://www.ndorms.ox.ac.uk/publications/103848
Identification and characterization of anti-osteoclastogenic peptides derived from the cytoplasmic tail of receptor activator...
Sphingosine 1-phosphate as a regulator of osteoclast differentiation and osteoclast-osteoblast coupling. Jiyoon Ryu; Hyung Joon ... Osteoclasts, cells that resorb bone, play a crucial role in bone metabolism. In this review, we... ... Studies of OC-STAMP in Osteoclast Fusion: A New Knockout Mouse Model, Rescue of Cell Fusion, and Transmembrane Topology. ... OC-STAMP, a multi-pass transmembrane protein, has been shown to be required for pre-osteoclast fusion and for... ...http://connection.ebscohost.com/c/articles/79824426/identification-characterization-anti-osteoclastogenic-peptides-derived-from-cytoplasmic-tail-receptor-activator-nuclear-factor-kappa-b
KEGG PATHWAY: Osteoclast differentiation
Osteoclast differentiation [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show description , User data ... The osteoclasts, multinucleared cells originating from the hematopoietic monocyte-macrophage lineage, are responsible for bone ... from RANK changes gene expression patterns through transcription factors like NFATc1 and characterizes the active osteoclast. ...http://www.genome.jp/kegg-bin/show_pathway?ko04380+K04371
RANKL-independent human osteoclast formation with APRIL, BAFF, NGF, IGF I and IGF II. - Nuffield Department of Orthopaedics,...
All five cytokines induced both osteoclast differentiation and activation with respect to the formation of significant numbers ... RANKL-independent human osteoclast formation with APRIL, BAFF, NGF, IGF I and IGF II. Share Share Share Share ... Osteoclasts, Humans, Nerve Growth Factor, Insulin-Like Growth Factor I, Insulin-Like Growth Factor II, RANK Ligand, B-Cell ... RANKL-independent human osteoclast formation with APRIL, BAFF, NGF, IGF I and IGF II. ...https://www.ndorms.ox.ac.uk/publications/245427
Interleukin-32 promotes osteoclast differentiation but not osteoclast activation. - The Arthritis Research UK Centre for...
Moreover, IL-32 was also capable of stimulating the release of IL-4 and IFN-gamma, two known inhibitors of osteoclast formation ... CONCLUSIONS/SIGNIFICANCE: This is the first in vitro report on the complex role of IL-32 on osteoclast precursors. Further ... it promoted the differentiation of osteoclast precursors into TRAcP+ VNR+ multinucleated cells expressing specific osteoclast ... although the number of newly generated osteoclast was increased, a significant decrease of the percentage of lacunar resorption ...https://oacentre.kennedy.ox.ac.uk/publications/103892
NLRP12 provides a critical checkpoint for osteoclast differentiation - BIOQUANT OSTEO
Older PostOsteoclast Deficiency Contributes to Temporomandibular Joint Ankylosed Bone Mass Formation ... The alternative or noncanonical nuclear factor kappa B (NF-κB) pathway regulates the osteoclast (OC) response to receptor ...https://osteo.bioquant.com/bibliography-entries/2016/4/7/nlrp12-provides-a-critical-checkpoint-for-osteoclast-differentiation
Osteoclast - Wikipedia
An osteoclast is a large multinucleated cell and human osteoclasts on bone typically have five nuclei and are about 150-200 µm ... In bone, osteoclasts are found in pits in the bone surface which are called resorption bays, or Howship's lacunae. Osteoclasts ... Once activated, osteoclasts move to areas of microfracture in the bone by chemotaxis. Osteoclasts lie in a small cavity called ... When osteoclast-inducing cytokines are used to convert macrophages to osteoclasts, very large cells that may reach 100 µm in ...https://en.wikipedia.org/wiki/Osteoclast
Fluoride Action Network | Fluoride & Osteoclasts
"We observed osteoclasts resorbing bone beneath osteoid seams, and fragments of osteoid isolated in the bone marrow. This type ... Fluoride, Osteoclasts, & Bone Resoprtion:. "An iliac crest bone biopsy revealed an increased amount of thick unmineralized ... Fluoride & Osteoclasts. SOURCE: Fluoride Action Network , May 24, 2012 , By Michael Connett ... "Osteoclasts were readily seen eroding bone. . . . Bone fluoride analysis showed 18 times the normal level of fluoride, 1.8%. ...https://fluoridealert.org/studies/osteoclasts/
Osteoclasts Control Osteoblast Chemotaxis via PDGF-BB/PDGF Receptor Beta Signaling
Methodology/Principal Findings Using in vitro cell systems, we show here that mature osteoclasts, but not their precursors, ... Conclusions/Significance We conclude that, in vitro mature osteoclasts control osteoblast chemotaxis via PDGF-bb/PDGFR-β ... Our subsequent functional analyses demonstrate that mature osteoclasts, whose platelet-derived growth factor bb (PDGF-bb) ... This may provide one key mechanism by which osteoclasts control bone formation in vivo. ...http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0003537
Impaired Differentiation of Osteoclasts in TREM-2-deficient Individuals | JEM
Origin of osteoclasts: mature monocytes and macrophages are capable of differentiating into osteoclasts under a suitable ... Impaired Differentiation of Osteoclasts in TREM-2-deficient Individuals. Marina Cella, Cecilia Buonsanti, Carey Strader, ... PYK2 in osteoclasts is an adhesion kinase, localized in the sealing zone, activated by ligation of alpha(v)beta3 integrin, and ... Impaired Differentiation of Osteoclasts in TREM-2-deficient Individuals. Marina Cella, Cecilia Buonsanti, Carey Strader, ...http://jem.rupress.org/content/198/4/645
osteoclast resorption assays
... From:. 'Montague, Donna C' Do you have a protocol for osteoclast resorption on dentine slices? I ... Resorption of dentine by isolated osteoclasts in vitro. Br Dent J 156:216-220. Help! Our provencial library doesn't have this ...http://www.histosearch.com/histonet/May02A/osteoclastresorptionassay.html
KEGG PATHWAY: Osteoclast differentiation - Mus musculus (mouse)
Osteoclast differentiation - Mus musculus (mouse) [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ... The osteoclasts, multinucleared cells originating from the hematopoietic monocyte-macrophage lineage, are responsible for bone ... from RANK changes gene expression patterns through transcription factors like NFATc1 and characterizes the active osteoclast. ...http://www.genome.jp/kegg-bin/show_pathway?mmu04380+21926
The Foreign Body Giant Cell Cannot Resorb Bone, But Dissolves Hydroxyapatite Like Osteoclasts
These results show that FBGCs have the capacity to dissolve the mineral phase of bone, similar to osteoclasts. However, they ... Despite similarities, an important distinction between these cell types is that osteoclasts can resorb bone, but it is unknown ... Both cell types were cultured on bovine bone slices and analyzed for typical osteoclast features, such as bone resorption, ... At the gene expression level, FBGCs and osteoclasts expressed similar levels of mRNAs that are associated with the dissolution ...http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0139564
The Ligand for Osteoprotegerin (OPGL) Directly Activates Mature Osteoclasts | JCB
osteoclasts. OPG/ OCIF. osteoprotegerin/osteoclast inhibitory factor. OPGL/ODF OPG. ligand/osteoclast differentiation factor ( ... Mature osteoclasts specifically bind OPGL and express RANK. Osteoclasts plated on FBS-coated coverslips were stained as ... Cell. 93: 165-176), but their effects on mature osteoclasts are not well understood. Using primary cultures of rat osteoclasts ... 1997) Phosphatidylinositol 3-kinase association with the osteoclast cytoskeleton, and its involvement in osteoclast attachment ...http://jcb.rupress.org/content/145/3/527
Bone degradation machinery of osteoclasts: An HIV-1 target that contributes to bone loss | PNAS
Supporting these results, Nef-transgenic mice exhibit an increased osteoclast density and bone defects, and osteoclasts derived ... HIV-1 exacerbates osteoclast functions. Brigitte Raynaud-Messina, Lucie Bracq, Maeva Dupont, Shanti Souriant, Shariq M. Usmani ... HIV-1 exacerbates osteoclast functions. Brigitte Raynaud-Messina, Lucie Bracq, Maeva Dupont, Shanti Souriant, Shariq M. Usmani ... Bone degradation machinery of osteoclasts: An HIV-1 target that contributes to bone loss. Brigitte Raynaud-Messina, Lucie Bracq ...http://www.pnas.org/content/115/11/E2556.short?rss=1