Involvement of the Src-cortactin pathway in podosome formation and turnover during polarization of cultured osteoclasts |...
6B) in untreated osteoclasts, as well as in osteoclasts overexpressing the different Src mutants. Osteoclasts expressed high ... Osteoclasts are large, multinucleated cells that adhere to bone via podosomes, and degrade it. During osteoclast polarization, ... RAW-derived osteoclasts were co-transfected with GFP-actin and corWT-flag or GFP-actin and cor3YF-flag. Cells were fixed and ... A) Control RAW osteoclasts, (B) cells co-expressing GFP-actin and SrcY527F, or (C) cells co-expressing GFP-actin and Src251 ...http://jcs.biologists.org/content/119/23/4878
Combined Effects of Soy Isoflavones and Docosahexaenoic Acid on Osteoclast Formation
This study investigated the combined effects of soy isoflavones and DHA on osteoclast formation. Mouse bone marrow cells were ... These results demonstrated that the combination of soy isoflavones and DHA decreased in osteoclast formation significantly, ... Combined Effects of Soy Isoflavones and Docosahexaenoic Acid on Osteoclast Formation. Shinichi Katsumata1, 2, , Gabrielle G. ... Combined Effects of Soy Isoflavones and Docosahexaenoic Acid on Osteoclast Formation. Journal of Food and Nutrition Research. ...http://www.sciepub.com/JFNR/abstract/6254
Gambogic acid inhibits osteoclast formation and ovariectomy-induced osteoporosis by suppressing the JNK, p38 and AKT signaling...
Taken together, our results demonstrate that GBA inhibits osteoclast formation in vitro and in vivo , suggesting that it is of ... Gambogic acid inhibits osteoclast formation and ovariectomy-induced osteoporosis by suppressing the JNK, p38 and AKT signaling ... Excessive osteoclast formation and bone resorption are key causes of osteoporosis. Natural compounds can serve as alternative ... Gambogic acid inhibits osteoclast formation and ovariectomy-induced osteoporosis by suppressing the JNK, p38 and AKT signaling ...http://www.biochemj.org/content/early/2015/06/10/BJ20150151
"Active caspase-3 is required for osteoclast differentiation" by K. H. Szymczyk, T. A. Freeman et al.
The Proceedings of the 8th ICCBMT). We noted that osteoclast numbers are decreased in long bones of procaspase-3 knockout mice ... Together these findings provide direct support for the hypothesis that caspase-3 activity is required for osteoclast ... we investigated the role of this protein in the differentiation of primary osteoclasts and RAW264.7 cells (Szymczyk KH, et al, ... 2005, Caspase-3 activity is necessary for RANKL-induced osteoclast differentiation. ...https://digitalcommons.pcom.edu/scholarly_papers/1588/
Atp6v1c1 is an essential component of the osteoclast proton pump and in F-actin ring formation in osteoclasts | Biochemical...
Atp6v1c1 is an essential component of the osteoclast proton pump and in F-actin ring formation in osteoclasts. Shengmei Feng, ... The present study demonstrates that Atp6v1c1 is an essential component of the osteoclast proton pump at the osteoclast ruffled ... Atp6v1c1 is an essential component of the osteoclast proton pump and in F-actin ring formation in osteoclasts ... Atp6v1c1 is an essential component of the osteoclast proton pump and in F-actin ring formation in osteoclasts ...http://www.biochemj.org/content/417/1/195
THU0031 Blocking αVβ3 integrin with cilengitide affects osteoclastogenesis in vitro and prevents collagen induced arthritis in...
... mononuclear osteoclasts (pre-osteoclasts) and TRAP+ multinucleated mature osteoclasts in the presence of macrophage-colony ... AlphaVbeta3 (αvβ3) Integrin is highly expressed in osteoclasts and its inhibition disturbs osteoclast function in vivo. However ... indicating an inhibiting effect on early stages of pre-osteoclast proliferation. Interestingly, with respect to osteoclast ... Results In vitro increasing concentrations of cilengitide (IC50: 250nM) dose-dependently reduced pre-osteoclast numbers on all ...http://ard.bmj.com/content/71/Suppl_3/163.3
c-Fms and the alphavbeta3 integrin collaborate during osteoclast differentiation. - Semantic Scholar
Finally, like high-dose MCSF, overexpression of c-Fos normalizes the number of beta(3)(-/-) osteoclasts in vitro, but not their ... In vitro, however, the number of beta(3)(-/-) osteoclasts is reduced because of arrested differentiation. This paradox suggests ... correlating with the increase in the osteoclast number. To identify components of the MCSF receptor that is critical for ... osteoclastogenesis in beta(3)(-/-) cells, we retrovirally transduced authentic osteoclast precursors with chimeric c-Fms ...https://www.semanticscholar.org/paper/c-Fms-and-the-alphavbeta3-integrin-collaborate-dur-Faccio-Takeshita/ae344a69fa8034be3370969248ae835f20965d47
The Mechanism of Osteoclast Differentiation Induced by IL-1 | The Journal of Immunology
Osteoclast formation. Murine osteoclasts were prepared from bone marrow cells or splenocytes as previously described (21, 22). ... Osteoclast precursor interaction with bone matrix induces osteoclast formation directly by an interleukin-1-mediated autocrine ... Herein, we demonstrate that IL-1 can directly induce osteoclast formation if IL-1RI is overexpressed in osteoclast precursor ... Although IL-1 has a synergistic effect on RANKL-induced osteoclast formation, IL-1 alone cannot induce osteoclast ...http://www.jimmunol.org/content/183/3/1862
Role of the osteoclast in cranial suture waveform patterning - Byron - 2006 - The Anatomical Record - Wiley Online Library
1995) that osteoclasts are only found in the diploic spaces of flat bones and Vastardis et al. (2004) that osteoclasts are not ... These raw osteoclast counts reveal that bone resorption reaches its climax at 6 weeks of age. By 6 months, osteoclast activity ... 4) and osteoclast knockdown data (Fig. 8), it seems clear that osteoclast activity is involved in sagittal suture patterning. ... Osteoclasts along straight margins follow a very similar pattern except that the maximum number of straight osteoclasts is ...http://onlinelibrary.wiley.com/doi/10.1002/ar.a.20322/full
Kahweol | CAS#6894-43-5 | includes inhibition of RANKL-induced osteoclast generation | MedKoo
It exhibits a wide variety of biological activities, including inhibiting RANKL-induced osteoclast generation, inducing cell ... It exhibits a wide variety of biological activities, including inhibiting RANKL-induced osteoclast generation, inducing cell ...http://www.medkoo.com/products/15202
Interleukin-6 and interleukin-11 support human osteoclast formation by a RANKL-independent mechanism. - Nuffield Department of...
In order to determine whether IL-6 and IL-11 could independently support human osteoclast formation, these factors were added ... are directly capable of inducing osteoclast formation by a RANKL-independent mechanism. ... are known to influence osteoclast formation and bone resorption. ... are directly capable of inducing osteoclast formation by a ... Interleukin-6 and interleukin-11 support human osteoclast formation by a RANKL-independent mechanism. Share Share Share Share ...https://www.ndorms.ox.ac.uk/publications/103838
Transforming growth factor-beta induces osteoclast formation in the absence of RANKL. - Nuffield Department of Orthopaedics,...
TGFbeta-induced osteoclast formation was qualitatively different from that induced by RANKL with numerous TRAP+/VNR+ ... TGFbeta is known to regulate RANKL-induced osteoclast formation and bone resorbing activity. In this study we sought to ... directly induce mononuclear phagocyte osteoclast precursors to differentiate into osteoclastic cells capable of lacunar ... determine whether TGFbeta could directly induce osteoclast formation by a RANKL-independent mechanism. We found that the ...https://www.ndorms.ox.ac.uk/publications/103848
Interleukin (IL)-6 induction of osteoclast differentiation depends on IL-6 receptors expressed on osteoblastic cells but not on...
... strikingly triggered osteoclast formation induced by IL-6. In this study, we examined the mechanism of osteoclast formation by ... 6 induction of osteoclast differentiation depends on IL-6 receptors expressed on osteoblastic cells but not on osteoclast ... 6 induction of osteoclast differentiation depends on IL-6 receptors expressed on osteoblastic cells but not on osteoclast ... In contrast, osteoclast progenitors in spleen cells from transgenic mice overexpressing human IL-6R were not able to ...http://jem.rupress.org/content/182/5/1461
RANKL-independent human osteoclast formation with APRIL, BAFF, NGF, IGF I and IGF II. - Nuffield Department of Orthopaedics,...
All five cytokines induced both osteoclast differentiation and activation with respect to the formation of significant numbers ... RANKL-independent human osteoclast formation with APRIL, BAFF, NGF, IGF I and IGF II. Share Share Share Share ... Osteoclasts, Humans, Nerve Growth Factor, Insulin-Like Growth Factor I, Insulin-Like Growth Factor II, RANK Ligand, B-Cell ... RANKL-independent human osteoclast formation with APRIL, BAFF, NGF, IGF I and IGF II. ...https://www.ndorms.ox.ac.uk/publications/245427
AB0103 Tacrolimus Inhibit IL-1β and ER Stress-Induced, Rankl-Mediated Osteoclastogenesis by Inhibiting Perk, Ire1, Grp78, Eif2a...
Results IL-1β and TG-induced ER stress increased the formation of osteoclasts by up-regulating the osteoclast specific signals ... The formation of osteoclasts was evaluated by tartrate-resistant acid phosphatase (TRAP) staining and resorption pit assay with ... Methods Bone marrow cells (BMCs) were obtained from 5-week-old male ICR mice and cultured to be differentiated into osteoclasts ... of the above effects of TAC on osteoclastogenesis were investigated by using RT-PCR and immunoblotting for osteoclast specific ...http://ard.bmj.com/content/73/Suppl_2/838.1
Interleukin-32 promotes osteoclast differentiation but not osteoclast activation. - The Arthritis Research UK Centre for...
Moreover, IL-32 was also capable of stimulating the release of IL-4 and IFN-gamma, two known inhibitors of osteoclast formation ... CONCLUSIONS/SIGNIFICANCE: This is the first in vitro report on the complex role of IL-32 on osteoclast precursors. Further ... it promoted the differentiation of osteoclast precursors into TRAcP+ VNR+ multinucleated cells expressing specific osteoclast ... although the number of newly generated osteoclast was increased, a significant decrease of the percentage of lacunar resorption ...https://oacentre.kennedy.ox.ac.uk/publications/103892
NLRP12 provides a critical checkpoint for osteoclast differentiation - BIOQUANT OSTEO
Older PostOsteoclast Deficiency Contributes to Temporomandibular Joint Ankylosed Bone Mass Formation ... The alternative or noncanonical nuclear factor kappa B (NF-κB) pathway regulates the osteoclast (OC) response to receptor ...https://osteo.bioquant.com/bibliography-entries/2016/4/7/nlrp12-provides-a-critical-checkpoint-for-osteoclast-differentiation
Osteoclast - Wikipedia
An osteoclast is a large multinucleated cell and human osteoclasts on bone typically have five nuclei and are about 150-200 µm ... In bone, osteoclasts are found in pits in the bone surface which are called resorption bays, or Howship's lacunae. Osteoclasts ... Once activated, osteoclasts move to areas of microfracture in the bone by chemotaxis. Osteoclasts lie in a small cavity called ... When osteoclast-inducing cytokines are used to convert macrophages to osteoclasts, very large cells that may reach 100 µm in ...https://en.wikipedia.org/wiki/Osteoclast
Fluoride Action Network | Fluoride & Osteoclasts
"We observed osteoclasts resorbing bone beneath osteoid seams, and fragments of osteoid isolated in the bone marrow. This type ... Fluoride, Osteoclasts, & Bone Resoprtion:. "An iliac crest bone biopsy revealed an increased amount of thick unmineralized ... Fluoride & Osteoclasts. SOURCE: Fluoride Action Network , May 24, 2012 , By Michael Connett ... "Osteoclasts were readily seen eroding bone. . . . Bone fluoride analysis showed 18 times the normal level of fluoride, 1.8%. ...https://fluoridealert.org/studies/osteoclasts/
KEGG PATHWAY: Osteoclast differentiation
Osteoclast differentiation [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show description , User data ... The osteoclasts, multinucleared cells originating from the hematopoietic monocyte-macrophage lineage, are responsible for bone ... from RANK changes gene expression patterns through transcription factors like NFATc1 and characterizes the active osteoclast. ...http://www.genome.jp/kegg-bin/show_pathway?ko04380+K04371
Osteoclasts Control Osteoblast Chemotaxis via PDGF-BB/PDGF Receptor Beta Signaling
Methodology/Principal Findings Using in vitro cell systems, we show here that mature osteoclasts, but not their precursors, ... Conclusions/Significance We conclude that, in vitro mature osteoclasts control osteoblast chemotaxis via PDGF-bb/PDGFR-β ... Our subsequent functional analyses demonstrate that mature osteoclasts, whose platelet-derived growth factor bb (PDGF-bb) ... This may provide one key mechanism by which osteoclasts control bone formation in vivo. ...http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0003537
Impaired Differentiation of Osteoclasts in TREM-2-deficient Individuals | JEM
Origin of osteoclasts: mature monocytes and macrophages are capable of differentiating into osteoclasts under a suitable ... Impaired Differentiation of Osteoclasts in TREM-2-deficient Individuals. Marina Cella, Cecilia Buonsanti, Carey Strader, ... PYK2 in osteoclasts is an adhesion kinase, localized in the sealing zone, activated by ligation of alpha(v)beta3 integrin, and ... Impaired Differentiation of Osteoclasts in TREM-2-deficient Individuals. Marina Cella, Cecilia Buonsanti, Carey Strader, ...http://jem.rupress.org/content/198/4/645
osteoclast resorption assays
... From:. 'Montague, Donna C' Do you have a protocol for osteoclast resorption on dentine slices? I ... Resorption of dentine by isolated osteoclasts in vitro. Br Dent J 156:216-220. Help! Our provencial library doesn't have this ...http://www.histosearch.com/histonet/May02A/osteoclastresorptionassay.html
KEGG PATHWAY: Osteoclast differentiation - Mus musculus (mouse)
Osteoclast differentiation - Mus musculus (mouse) [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ... The osteoclasts, multinucleared cells originating from the hematopoietic monocyte-macrophage lineage, are responsible for bone ... from RANK changes gene expression patterns through transcription factors like NFATc1 and characterizes the active osteoclast. ...http://www.genome.jp/kegg-bin/show_pathway?mmu04380+21926
Effect of Tumor Necrosis Factor Inhibitor Therapy on Osteoclasts Precursors in Rheumatoid Arthritis
"Infliximab acts directly on human osteoclast precursors and enhances osteoclast formation induced by receptor activator of ... S. L. Teitelbaum and F. P. Ross, "Genetic regulation of osteoclast development and function," Nature Reviews Genetics, vol. 4, ... Effect of Tumor Necrosis Factor Inhibitor Therapy on Osteoclasts Precursors in Rheumatoid Arthritis. Inês P. Perpétuo,1 Joana ... L. K. Osier, S. N. Popoff, and S. C. Marks Jr., "Osteopetrosis in the toothless rat: failure of osteoclast differentiation and ...https://www.hindawi.com/journals/bmri/2017/2690402/ref/