Action of phorbol myristate acetate (PMA) at fertilization of mouse oocytes in vitro | Development
Effectiveness of blocking cytokinesis is dependent on the timing of exposure of oocytes to PMA action. When oocytes are ... Phorbol ester (PMA) in concentration 5 and 10ng ml−1 blocks cytokinesis of the second maturation division in mouse oocytes. ... Karyokinesis is not impaired and digynic triploid oocytes are obtained which undergo first cleavage division. ... Action of phorbol myristate acetate (PMA) at fertilization of mouse oocytes in vitro ...http://dev.biologists.org/content/90/1/171
Ultrastructural changes in oocytes during folliculogenesis in domestic mammals | Journal of Ovarian Research | Full Text
D: Oocyte after 18 hours of IVM showing cortical granules (arrows) aligned to the plasma membrane. E: oocyte after 18 hours of ... The number of cytoplasmic vesicles increases in active oocytes in cattle  and buffaloes , occupying most of the oocyte ... Ultrastructural events during oocyte maturation in bovine. A: Oocyte from tertiary follicle with intact germinal vesicle (GV), ... B: Pig primary follicle with several lipid droplets in the oocyte and granulosa cells cytoplasm. O: oocyte, Nu: nucleus, GC: ...https://ovarianresearch.biomedcentral.com/articles/10.1186/s13048-014-0102-6
GnRH agonist triggering affects the kinetics of embryo development: a comparative study | Journal of Ovarian Research | Full...
Some recent studies focused on the oocyte maturation rate after hCG and GnRHa triggering, and found that the number of oocytes ... Oocyte retrieval and intracytoplasmic sperm injection. Transvaginal oocyte retrieval was performed 35 h after triggering. ... In conclusion, the method used to trigger oocyte maturation seems to affect the dynamic parameters of early oocyte and embryo ... Our results confirmed that the oocyte-triggering agent affected the early developmental kinetics of oocytes and embryos. ...https://ovarianresearch.biomedcentral.com/articles/10.1186/s13048-016-0229-8
Effect of vitrification of mouse oocyte on the behavior of adult offspring. - Semantic Scholar
... and compared to that of offspring from fresh oocytes. RESULTS Offspring produced by vitrified oocytes showed normal motor ... STUDY DESIGN Oocytes from mice were vitrified, warmed and inseminated, and two-cell embryos were transferred to foster mothers ... In addition, offspring from vitrified oocytes did not exhibit alterations in emotional behavior. CONCLUSION No alterations were ... found in the behavioral characterization of adult offspring from vitrified oocytes compared with those from fresh oocytes. ...https://www.semanticscholar.org/paper/Effect-of-vitrification-of-mouse-oocyte-on-the-beh-Liu-Jia/ab61bf68dbc9e8ddac434f3d71dfc43a40f95072
Modulation of meiotic arrest in mouse oocytes by guanyl nucleotides a" by S M. Downs, R Buccione et al.
... action was inhibitory in denuded oocytes. AlF4- stimulated GVB in both cumulus cell-enclosed oocytes and denuded oocytes when ... Cumulus cell-free mouse oocytes (denuded oocytes) were maintained in meiotic arrest in a transient and dose-dependent manner ... cell-enclosed oocytes or denuded oocytes when meiotic arrest was maintained with dibutyryl cAMP (dbcAMP) or hypoxanthine. ... in cumulus cell-enclosed oocytes. Cholera toxin or FSH alone each stimulated the synthesis of cAMP in oocyte-cumulus cell ...http://mouseion.jax.org/stfb1990_1999/262/
British Library EThOS: Pig oocyte activation and developmental competence of parthenogenetic activated oocytes : in vitro and...
Pig oocyte activation and developmental competence of parthenogenetic activated oocytes : in vitro and in vivo studies ... Therefore studies presented in this thesis aimed at the establishment of an in vitro culture system for pig oocyte maturation ... activation protocol for pig oocytes was optimized and also tested by monitoring in vivo development of activated pig oocytes. ... and concentrations of Ca2+ and Mg2+ in activation medium and diploidization of activated oocytes. These experiments were ...http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.664239
I originated the concept of an oocyte-granulosa cell regulatory loop in which bi-directional communication between the oocyte ... I found that oocytes are not simply passive recipients of nutrients and signals from ovarian follicular somatic cells, as once ... I also achieved the first complete development of mammalian oocytes in vitro. This included in vitro initiation of primordial ... The focus of my research is on the complex interactions between developing oocytes and their companion follicular somatic cells ...http://www.nasonline.org/member-directory/members/20024872.html
LH frees oocytes from meiotic arrest | Development
Meiosis in mammalian oocytes pauses in prophase until luteinizing hormone (LH) releases this arrest. One suggestion is that LH ... thus blocking the transmission of a meiosis-inhibitory signal to oocytes. Now, Norris and co-workers show that LH-induced MAP ... occurs in oocytes. MAP kinase-dependent phosphorylation of connexin 43 causes this decreased permeability, they report. However ... does this by closing the gap junctions between the somatic cells that surround the oocyte, ...http://dev.biologists.org/content/135/19/e1905
KEGG PATHWAY: Progesterone-mediated oocyte maturation - Xenopus laevis (African clawed frog)
Progesterone-mediated oocyte maturation - Xenopus laevis (African clawed frog) [ Pathway menu , Organism menu , Pathway entry ... Xenopus oocytes are naturally arrested at G2 of meiosis I. Exposure to either insulin/IGF-1 or the steroid hormone progesterone ... This process is termed oocyte maturation. The transition is accompanied by an increase in maturation promoting factor (MPF or ... breaks this arrest and induces resumption of the two meiotic division cycles and maturation of the oocyte into a mature, ...http://www.genome.jp/kegg-bin/show_pathway?map=xla04914&show_description=show
Voltage-operated channels induced by foreign messenger RNA in Xenopus oocytes | Proceedings of the Royal Society of London B:...
Voltage-operated channels induced by foreign messenger RNA in Xenopus oocytes. C. B. Gundersen, R. Miledi, F. R. S., I. Parker ... Voltage-operated channels induced by foreign messenger RNA in Xenopus oocytes. C. B. Gundersen, R. Miledi, F. R. S., I. Parker ... Poly(A)+ messenger RNA (mRNA) extracted from rat brains or from cat muscles was injected into Xenopus laevis oocytes. This led ... Voltage-operated channels induced by foreign messenger RNA in Xenopus oocytes Message Subject (Your Name) has sent you a ...http://rspb.royalsocietypublishing.org/content/220/1218/131
Oocyte development and maturity classification of boarfish (Capros aper) in the Northeast Atlantic - DTU Orbit
Oocyte development and maturity classification of boarfish (Capros aper) in the Northeast Atlantic. Publication: Research - ... Oocyte development and maturity classification of boarfish (Capros aper) in the Northeast Atlantic. - ICES Journal of Marine ... This study presents the first detailed investigation of the oocyte development and maturity classification of boarfish, Capros ... Once spawning ceased the remaining mature oocytes were resorbed. Preliminary analysis of reproductive strategy indicates that ...http://orbit.dtu.dk/en/publications/oocyte-development-and-maturity-classification-of-boarfish-capros-aper-in-the-northeast-atlantic
Primary oocyte synonyms, primary oocyte antonyms - FreeThesaurus.com
Antonyms for primary oocyte. 1 word related to oocyte: gametocyte. What are synonyms for primary oocyte? ... oocyte. (redirected from primary oocyte). Also found in: Dictionary, Medical, Encyclopedia.. Related to primary oocyte: ... In female gonads, ascines contained secondary oocytes and lesser quantities of oogonia and primary oocytes (Fig.. Reproduction ... Primary oocyte synonyms, primary oocyte antonyms - FreeThesaurus.com https://www.freethesaurus.com/primary+oocyte ...https://www.freethesaurus.com/primary+oocyte
Functional Characterization of Human Breast Cancer Resistance Protein (BCRP, ABCG2) Expressed in the Oocytes of Xenopus laevis ...
BCRP Expressed in X. laevis oocytes Localizes to the Oocyte Surface. Oocytes were injected with cRNA coding for the wild-type ( ... R482T/S187T-expressing oocytes (. ), or R482T/S187A-expressing oocytes (gray ). Oocytes were injected with 50 μl of each cRNA ( ... oocytes were injected with 50 nl of water per oocyte (hereafter referred to as control oocytes) or 50 nl of 1 μg/μl cRNA ... injected oocytes was 3.79 ± 0.15 pmol/oocyte, whereas that in control was 4.55 ± 0.31 pmol/oocyte. [3H]MX accumulation in ...http://molpharm.aspetjournals.org/content/64/6/1452
Role for capping protein in mouse oocytes | Journal of Cell Science
This process is involved in the asymmetric division of mouse oocytes, but the role of capping protein itself is poorly ... The authors first show that capping protein mainly localises to the oocyte cytoplasm during maturation. Knockdown of capping ... Taken together, these data suggest that capping protein has an essential role in actin-driven processes during oocyte ... Expression of the capping-protein-binding region of CARMIL, the capping-protein antagonist, decreased the oocyte maturation ...http://jcs.biologists.org/content/128/1/e0104
In vitro maturation and fertilization of oocytes isolated from aged m" by J J. Eppig and M O'Brien
CONCLUSION: Oocytes from virgin aged mice undergo normal cytoplasmic maturation in vitro. Higher percentages of oocytes from ... RESULTS: Although the number of oocytes recovered from mice was greatly reduced with increasing age, the percentage of oocytes ... If so, oocyte maturation in vitro could be used as a strategy to rescue valuable genetic resources. ... Therefore, in vitro maturation and fertilization of oocytes could be used to rescue valuable genetic resources that might ...https://mouseion.jax.org/stfb1990_1999/665/
Intracellular acidification delays hormonal G2/M transition and inhibits G2/M transition triggered by thiophosphorylated MAPK...
When injected in an immature recipient oocyte, egg cytoplasm triggers GVBD through MPF auto-amplification, independently of ... phosphorylation induced by progesterone were also delayed by incubation of oocyte in NH(4)Cl. The delay induced by NH(4)Cl was ... Xenopus oocyte maturation is analogous to G2/M transition and characterized by germinal vesicle breakdown (GVBD), spindle ... Calcium Dynamics During Physiological Acidification in Xenopus Oocyte. *Matthieu Marin. , Chantal Sellier. , +4 authors. Jean- ...https://www.semanticscholar.org/paper/Intracellular-acidification-delays-hormonal-G2-M-t-Sellier-Bodart/b8f13290534b50af2f6247f23739cbff50348129
Granules that act as molecular timers discovered in vertebrate oocytes | Hokkaido University
Granules of cyclinB1 mRNA (green) in an immature mouse oocyte. The nuclei of the oocyte and follicular cells are stained blue. ... In the process of oocyte maturation in particular, the timing of protein synthesis is regulated to within a few minutes, and ... This study elucidated that the mRNA accumulated in immature vertebrate oocytes forms granules, and that these granules act as ... Granules containing mRNA molecules are present in vertebrate oocytes, and these have been elucidated to act as molecular timers ...https://www.global.hokudai.ac.jp/blog/granules-that-act-as-molecular-timers-discovered-in-vertebrate-oocytes/
Triggering final oocyte maturation - Eugonia
Triggering final oocyte maturation. The day when sufficient follicle maturation is observed you will be informed of the exact ... Home In vitro fertilisation Course of treatment program Triggering final oocyte maturation ... This injection is human chorionic gonadotrophin which mimics the LH action and induces final oocyte maturation and ovulation. ... time for your last injection which will induce final oocyte maturation and ovulation (Pregnyl or Profasi or Ovitrelle) as well ...http://www.eugonia.com.gr/en/ivf/treatment-program/triggering-final-oocyte-maturation/
Surrogate Genetics in the Frog Oocyte | SpringerLink
Gurdon, J.B. and Brown, D.D. (1978). The transcription of 5S DNA injected into Xenopus oocytes. Dev. Biol., 67, 346.PubMed ... Xenopus Laevis tRNA Gene Xenopus Oocyte Histone Gene Animal Pole These keywords were added by machine and not by the authors. ... Lane, C.D. and Knowland, J. (1975). The injection of RNA into living cells: The use of frog oocytes for the assay of mRNA and ... Probst, E., Kressmann, A. and Birnstiel, M.L. (1979). Expression of sea urchin genes in the oocyte of Xenopus laevis. J. Mol. ...https://link.springer.com/chapter/10.1007/978-1-4684-3707-2_17
Intrafollicular Mechanisms Regulating Oocyte Maturation | SpringerLink
Arrest and reinitiation of oocyte maturation occur in association with the processes of folliculogenesis and ovulation. Somatic ... Schuetz AW (1967a) Effect of steroids on the germinal vesicle of oocytes of the frog (Rana pipiens) In vitro Proc. Soc. Exp. ... Schuetz AW (1967b) Action of hormones in germinal vesicle breakdown in frog (Rana pipiens) oocytes. J. Exp. Zool. 166: 347-354. ... Maturation of oocytes, without evident hormone stimulation, can be achieved in the presence or absence of the somatic cells, ...https://link.springer.com/chapter/10.1007/978-3-642-83965-8_5
Urchin oocyte cDNA library (query)
... Dan Jacobson danj at welchgate.welch.jhu.edu Fri Apr 2 11:39:20 EST 1993 *Previous message ... Derived from whole mRNA of the oocyte of the urchin species , L. pictus or S. purpuratus. , ,2) cDNAs are ligated into ...http://www.bio.net/bionet/mm/methods/1993-April/004985.html
frozen frog oocytes
... From:. Andrea Grantham I'm trying to section Xenopus oocytes frozen in OCT with poor results. Lots of ... Is there anybody out in histonetland who may have done some sectioning of frog oocytes or something similar? I actually found a ... Does anybody know an optimal temp for cutting frog oocytes? Thanks for any help. Andi Grantham ...http://www.histosearch.com/histonet/Oct02/frozenfrogoocytes.html
Biomedical Cell Biology seminar: Actin + Oocytes: Old Love - New Affairs
Dr Schuh will also talk about the actin-dependent mechanism of asymmetric spindle positioning in mouse oocytes. ... of the vesicle-actin network is driven by recruitment of vesicles to the plasma membrane in the periphery of the oocyte. Being ... an unexpected actin-dependent but microtubule-independent mechanism for long-range transport of vesicles in mouse oocytes. ...https://warwick.ac.uk/insite/events/events/biomedical_seminar_250112/
Retrieval of Mouse Oocytes | Protocol (Translated to Hebrew)
Duselis, A. R., Vrana, P. B. Retrieval of Mouse Oocytes. J. Vis. Exp. (3), e185, doi:10.3791/185 (2007). ...https://www.jove.com/video/185/-?language=Hebrew