Role of Chromosome Loss in ras/myc-induced Syrian Hamster Tumors | Cancer Research
... myc oncogenes were expressed. Furthermore, radioimmune precipitation with a monoclonal antibody to p21res of [35S]methionine- ... dodecyl sulfate electrophoresis showed that the suppressed hybrid cells contained high levels of the mutated ras protein. These ... Role of Chromosome Loss in ras/myc-induced Syrian Hamster Tumors. Mitsuo Oshimura, Minoru Koi, Nobuyoshi Ozawa, Osamu Sugawara ... Role of Chromosome Loss in ras/myc-induced Syrian Hamster Tumors. Mitsuo Oshimura, Minoru Koi, Nobuyoshi Ozawa, Osamu Sugawara ...http://cancerres.aacrjournals.org/content/48/6/1623
Knock-in of Mutant K-ras in Nontumorigenic Human Epithelial Cells as a New Model for Studying K-ras-Mediated Transformation |...
S2A). The p53 pathway was also functionally examined by assessing increases in p53 and p21 protein by Western blot after cells ... Targeted knock-in of oncogenic K-rasV12. A, scheme for targeted knock-in of the K-rasV12 oncogene. The endogenous K-ras gene ... rasWT, and LXSN (left) and parent, K-rasV12 #1, K-rasV12 #2, K-rasWT, and LXSN (right) in each time point are near 1.0 and ... rasV12 compared with the endogenous WT K-ras gene. Using Western blot analysis, the amount of K-Ras protein in knock-in clones ...http://cancerres.aacrjournals.org/content/67/18/8460.long
Promoter swapping between the genes for a novel zinc finger protein and β-catenin in pleiomorphic adenomas with t(3;8)(p21;q12 ... Identification of a provirally activated c-Ha-ras oncogene in an avian nephroblastoma via a novel procedure: cDNA cloning of a ... c-Ha-ras, nov, and sprouty2: examples of rare targets for provirus integration. c-Ha-ras and nov were earlier reported to be ... Analysis of cVIS in c-Ha-ras, nov, and sprouty2 loci. A, precise structure of MAV-c-Ha-ras chimeric mRNAs. Dashed lines, the ...http://cancerres.aacrjournals.org/content/66/1/78
Oxidative Stress Induces Premature Senescence by Stimulating Caveolin-1 Gene Transcription through p38 Mitogen-Activated...
... such as G-protein α subunit, Ras, nitric oxide synthase, protein kinase C, and protein kinase A ( 2, 7, 10, 16- 25). However, ... caveolin-1 mRNA and protein expression are lost or reduced during cell transformation by activated oncogenes, such as v-Abl and ... we evaluated the effect of inhibition of p38 MAPK on the cellular levels of p21WAF1/Cip1. Figure 3F shows that p21WAF1/Cip1 ... Activation of Ras-MAP kinase and protein kinase a signaling cascades transcriptionally down-regulates caveolin-1 promoter ...http://cancerres.aacrjournals.org/content/66/22/10805
McKenzie PP, Guichard SM, Middlemas DS, Ashmun RA, Danks MK, Harris LC. Wild-type p53 can induce p21 and apoptosis in ... Mantovani F, Banks L. The human papillomavirus E6 protein and its contribution to malignant progression. Oncogene 2001; 20: ... p21 (p21WAF1), BTG2, WIP1, and p48, by comparison with the up-regulation of these genes in the parental IMR-32 cells. This ... 2B). This contrasted with the vector controls, SH-EP/CMV1 and SH-EP/CMV2, both of which strongly induced p53 and p21 after drug ...http://cancerres.aacrjournals.org/content/67/21/10351
High-Throughput Tyrosine Kinase Activity Profiling Identifies FAK as a Candidate Therapeutic Target in Ewing Sarcoma | Cancer...
A, protein levels measured by Western immunoblotting for AKT/mTOR pathway proteins in A673 and TC32 cells serum-starved ... Integrin-mediated signal transduction linked to Ras pathway by GRB2 binding to focal adhesion kinase. Nature 1994;372:786-91. ... Nuclear expression of p53, p21 and cyclin D1 is increased in bronchioloalveolar carcinoma. Histopathology 1999;34:439-46. ... pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions. Proc Natl Acad Sci U S A 1992;89: ...http://cancerres.aacrjournals.org/content/73/9/2873.long
5B), whereas total levels of both proteins remained unchanged (data not shown). Moreover, the tumor suppressor p21, which acts ... Chronic pancreatitis is essential for induction of pancreatic ductal adenocarcinoma by K-Ras oncogenes in adult mice. Cancer ... and protein content was assessed using Protein Assay Reagent (Thermo Scientific). For Western blotting, 10 μg proteins were ... Reverse phase protein array analyses. Reverse phase protein array (RPPA) technology was used for high-content analysis of ...http://cancerres.aacrjournals.org/content/76/1/96
Requirement for Rac1 in a K-ras-Induced Lung Cancer in the Mouse | Cancer Research
... such as the anti-apoptotic protein BAD, via the p21-activated kinase 1 ( 22, 23). It is also possible that the effect of Rac1 ... Tang Y, Zhou H, Chen A, Pittman RN, Field J. The Akt proto-oncogene links Ras to Pak and cell survival signals. J Biol Chem ... 16) were crossed to the LSL-K-rasG12D animals to generate LSL-K-rasG12D;Rac1flox/flox and control LSL-K-rasG12D;Rac1flox/+ mice ... Tumors were excised from the LSL-K-rasG12D;Rac1flox/+ mice at 12-wk (lanes 1-4) and LSL-K-rasG12D;Rac1flox/flox mice at 18-wk ( ...http://cancerres.aacrjournals.org/content/67/17/8089
Expression of Endogenous Oncogenic V600EB-raf Induces Proliferation and Developmental Defects in Mice and Transformation of...
5, 11, 12). Thus, in a similar way to RAS oncogenes that are detected in 15% to 30% of human cancers (13), B-RAF mutations are ... Right, pooled weight data from four spleens of LSL-B-rafV600E ± Mx1-Cre mice at P21. LSL-B-rafV600E + Mx1-Cre mice have a mean ... Protein lysates were harvested from various tissues and subjected to (A) B-Raf kinase assays and (B) Western blot analysis with ... Tumor induction by an endogenous K-ras oncogene is highly dependent on cellular context. Cancer Cell 2003;4:111-20. ...http://cancerres.aacrjournals.org/content/65/24/11493.long
Role of Mitogen-activated Protein Kinase/Extracellular Signal-regulated Kinase Cascade in Gonadotropin-releasing Hormone...
... and SH3-dependent protein-protein interactions among tyrosine-phosphorylated receptor, Shc, Grb2, and Sos, resulting in p21ras ... Oncogene, 10: 849-855, 1995.. OpenUrlPubMed. *↵ Koch W. J., Hawes B. E., Allen L. F., Lefkowitz R. J. Direct evidence that Gi- ... Immunocomplexes were precipitated with protein A-Sepharose or protein G plus/protein A-agarose and washed three times with HNTG ... and SH3-dependent protein-protein interactions among tyrosine-phosphorylated receptors, Shc, Grb2, and Sos, resulting in a Ras- ...http://cancerres.aacrjournals.org/content/59/20/5133
Toyoshima H., Hunter T. p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21. Cell, 78: 67-74, ... The PTEN/MMAC1 tumor suppressor induces cell death that is rescued by the AKT/protein kinase B oncogene. Cancer Res., 58: 5667- ... AFX-like Forkhead transcription factors mediate cell-cycle regulation by Ras and PKB through p27kip1. Nature (Lond.), 404: 782- ... Protein concentration was quantitated using a modified Bradford assay (Bio-Rad protein assay), and 50 μg of total protein were ...http://cancerres.aacrjournals.org/content/61/11/4569
Bargmann CI, Hung MC, Weinberg RA Multiple independent activations of the neu oncogene by a point mutation altering the ... elevated Cyclin D1 protein levels, and phosphorylation of retinoblastoma protein. These bitransgenic mammary tumors also ... Rossig L, Jadidi AS, Urbich C, Badorff C, Zeiher AM, Dimmeler S Akt-dependent phosphorylation of p21(Cip1) regulates PCNA ... 4A ⇓ , Lanes 1, 4-7). Consistent with previous studies (20 , 28) , ErbB-2 protein levels were quite low in 8-10-week-old NDL2-5 ...http://cancerres.aacrjournals.org/content/64/9/3171
A number of oncogenes, including K-ras, Myc, ERBB gene family, and MET can be overexpressed or mutated in lung cancer, as well ... The focal adhesion protein paxillin is a target of a number of oncogenes involved in key signal transduction and important in ... p21-activated kinases in cancer. Nat Rev Cancer 2006; 6: 459-71. ... Detection of ras gene mutations in human lung cancer: ... Expression of the focal adhesion protein paxillin in lung cancer and its relation to cell motility. Oncogene 1999; 18: 67-77. ...http://cancerres.aacrjournals.org/content/68/1/132
Inhibition of RAS-Mediated Transformation and Tumorigenesis by Targeting the Downstream E3 Ubiquitin Ligase Seven in Absentia...
... profiling by DNA microarray analysis in mouse embryonic fibroblasts transformed by rasV12 mutated protein and the E1A oncogene ... Rabbit-α-p21 was purchased from Santa Cruz Biotechnology and used at 1:1,000 dilution. Mouse-α-HIF1α was purchased from BD ... 6A and B). The differential requirement for SIAH in RAS signaling in cells expressing wild-type RAS versus oncogenic RAS could ... RAS proteins are evolutionarily conserved small GTPases that shuffle between a GTP-bound active state and GDP-bound inactive ...http://cancerres.aacrjournals.org/content/67/24/11798.full
Apoptosis Repressor with Caspase Recruitment Domain Contributes to Chemotherapy Resistance by Abolishing Mitochondrial Fission...
Gustafsson AB, Sayen MR, Williams SD, Crow MT, Gottlieb RA. TAT protein transduction into isolated perfused hearts: TAT- ... p300 regulates p53-dependent apoptosis after DNA damage in colorectal cancer cells by modulation of PUMA/p21 levels. Proc Natl ... Oncogene 2002; 21: 1-8.. OpenUrlCrossRefPubMed. *↵ Li P, Nijhawan D, Budihardjo I, et al. Cytochrome c and dATP-dependent ... Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins. Cell 2000; ...http://cancerres.aacrjournals.org/content/69/2/492
... and activator protein 2 (45) to the p21 promoter. In our studies, although p53 was mutated, p21 was up-regulated in IRF-1- ... Nozawa H, Oda E, Nakao K, et al Loss of transcription factor IRF-1 affects tumor susceptibility in mice carrying the Ha-ras ... In fact, ectopic expression of IRF-1 can revert oncogene-transformed cultured cell lines to a normal phenotype (5) , whereas ... Harper JW, Adami G, Wein N, Keyomarsi K, Elledge S The p21 cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin- ...http://cancerres.aacrjournals.org/content/64/22/8381
Protein Kinase Cι Is Required for Pancreatic Cancer Cell Transformed Growth and Tumorigenesis | Cancer Research
The KRAS proto-oncogene is mutated in ,90% of PDAC. Protein kinase Cι (PKCι) is required for the oncogenic Ras-mediated ... Suppression of Ki-ras p21 levels leading to growth inhibition of pancreatic cancer cell lines with Ki-ras mutation but not ... Evidence that atypical protein kinase C-λ and atypical protein kinase C-ζ participate in Ras-mediated reorganization of the F- ... Protein kinase Cι is required for Ras transformation and colon carcinogenesis in vivo. J Cell Biol 2004;164:797-802. ...http://cancerres.aacrjournals.org/content/70/5/2064
Targeting the Raf-MEK-ERK mitogen-activated protein kinase cascade for the treatment of cancer. Oncogene 2007;26:3291-310. ... Early G1 growth arrest of hybridoma B cells by DMSO involves cyclin D2 inhibition and p21[CIP1] induction. Oncogene 1998;17: ... Glycolysis and methylaminoisobutyrate uptake in rat-1 cells transfected with ras or myc oncogenes. Proc Natl Acad Sci U S A ... Regulation of c-myc expression by Ras/Raf signalling. Oncogene 1998;16:211-6. ...http://cancerres.aacrjournals.org/content/73/13/4039
EIF3i promotes colon oncogenesis by regulating COX-2 protein synthesis and beta-catenin activation. Oncogene 2014;33:4156-63. ... Survivin initiates procaspase 3/p21 complex formation as a result of interaction with Cdk4 to resist Fas-mediated cell death. ... Stahel RA, *et al.. A novel antisense oligonucleotide targeting survivin expression induces apoptosis and sensitizes lung ... Western blot analysis of survivin protein in different human cancer cell lines using 10 μg proteins of total cell lysate each. ...http://cancerres.aacrjournals.org/content/76/2/453
Activation of Akt/protein kinase B by G protein-coupled receptors.. A role for α and β γ subunits of heterotrimeric G proteins ... Because both ICI and IGF-I have been reported to regulate p27Kip1 and p21Waf1 levels in MCF-7 cells (31 , 32) , we examined the ... Hu Q., Klippel A., Muslin A. J., Fantl W. J., Williams L. T. Ras-dependent induction of cellular responses by constitutively ... Oncogene, 19: 4746-4753, 2000. ... and protein was quantitated using the Bradford protein assay ( ...http://cancerres.aacrjournals.org/content/62/14/3985
... and proto-oncogenes converge on the G protein RAS, which activates the serine/threonine kinase RAF, which activates the MAPK ... van den Bos C, Silverstetter S, Murphy M, Connolly T. p21(cip1) rescues human mesenchymal stem cells from apoptosis induced by ... phospho-stress-activated protein kinase (SAPK)/JNK (Thr183/Tyr185), SAPK/JNK, phospho-mitogen-activated protein kinase (MAPK)/ ... Lee WH, Morton RA, Epstein JI, et al. Cytidine methylation of regulatory sequences near the π-class glutathione S-transferase ...http://cancerres.aacrjournals.org/content/65/20/9485
Gene Expression Profiling of Liposarcoma Identifies Distinct Biological Types/Subtypes and Potential Therapeutic Targets in...
... or known oncogenes [RAB11B (RAS oncogene family)], GTPase signal transduction, SSPN-sarcospan (Kras oncogene-associated gene ... and p21 (Santa Cruz Biotechnology, Inc.). The equal loading of protein was confirmed by Amido Black staining (Sigma Chemical) ... Total protein concentration of the lysates was measured by Bio-Rad protein assay (Bio-Rad Laboratories), and equal amounts of ... Evidence that the G1-S and G2-M transitions are controlled by different cdc2 proteins in higher eukaryotes. Cell 1991; 66: 731- ...http://cancerres.aacrjournals.org/content/67/14/6626.full
Neurotensin Induces Protein Kinase C-dependent Protein Kinase D Activation and DNA Synthesis in Human Pancreatic Carcinoma Cell...
Activation of protein kinase Cα inhibits growth of pancreatic cancer cells via p21(cip)-mediated G(1) arrest. J. Cell Sci., 113 ... Giehl K., Skripczynski B., Mansard A., Menke A., Gierschik P. Growth factor-dependent activation of the Ras-Raf-MEK-MAPK ... and cellular oncogenes (17 , 18) . Molecular cloning has demonstrated the existence of multiple related PKC isoforms, which are ... Rapid protein kinase D translocation in response to G protein-coupled receptor activation. Dependence on Protein Kinase C. J. ...http://cancerres.aacrjournals.org/content/62/6/1632
... from inhibition of the farnesylation of proteins other than Ras ( 3, 4). More than 10 uncharacterized isoprenylated proteins ... Servais P, Gulbis B, Fokan D, Galand P. Effects of the farnesyltransferase inhibitor UCF-1C/manumycin on growth and p21-ras ... Recombinant ATM protein complements the cellular A-T phenotype. Oncogene 1997; 15: 159-67. ... Fritz G, Kaina B, Aktories K. The ras-related small GTP-binding protein RhoB is immediate-early inducible by DNA damaging ...http://cancerres.aacrjournals.org/content/65/9/3671
P21-activated kinase is required for mitotic progression and regulates Plk1. Oncogene 2008;27:4900-8. ... Three of the 7 validated genes were Ras superfamily GTPases (RhoJ, Arl4a, Rnd2), implicating a novel role for Ras superfamily ... Chemical genetic screen for AMPKalpha2 substrates uncovers a network of proteins involved in mitosis. Mol Cell 2011;44:878-92. ... Pak protein kinases and their role in cancer. Cancer Metastasis Rev 2009;28:51-63. ...http://cancerres.aacrjournals.org/content/72/21/5516