BIOLOGICAL SAMPLE TARGET CLASSIFICATION, DETECTION AND SELECTION METHODS, AND RELATED ARRAYS AND OLIGONUCLEOTIDE PROBES -...
BIOLOGICAL SAMPLE TARGET CLASSIFICATION, DETECTION AND SELECTION METHODS, AND RELATED ARRAYS AND OLIGONUCLEOTIDE PROBES. United ... together with related arrays and oligonucleotide probes.. Inventors:. Gardner, Shea N. (Oakland, CA), Jaing, Crystal J. ( ... together with related arrays and oligonucleotide probes. ...http://techportal.eere.energy.gov/application.do/ID=19987
Oligonucleotide Probes for Multiplex Genetic Analyses
This project discusses about Oligonucleotide Probes, Multiplex Genetic Analyses, Multiplex nucleic acid analyses, Genetic ... Oligonucleotide probes are effective tools for the research of nucleic acids. ... variation, Different Types of nucleic acid analyses, multiplex oligonucleotide design, ... In fact techniques utilizing oligonucleotide probes are effective tools for the research of nucleic acids. In the past few ...http://www.projectsparadise.com/oligonucleotide-probes-multiplex-genetic-analyses/
Patent US5667972 - Method of sequencing of genoms by hybridization of oligonucleotide probes - Google Patents
The sequence of a given DNA fragment is read by the hybridization and assembly of positively hybridizing probes through ... with about 50,000 to 100,000 groups of probes, the main type of which is (A,T,C,G)(A,T,C,G)N8(A,T,C,G), information for ... The conditions under which oligonucleotides hybridize only with entirely homologous sequences are recognized. ... wherein a portion of the labeled oligonucleotide probe sequence at an end of the labeled oligonucleotide probe is the same as ...http://www.google.com/patents/US5667972?dq=patent:6144888
Internal labeling of oligonucleotide probes by Diels-Alder cycloaddition - Strathprints
Graham, D. and Grondin, A. and McHugh, C. and Fruk, L. and Smith, W.E. (2002) Internal labeling of oligonucleotide probes by ... dna, oligonucleotide probes, cycloaddition, Chemistry, Biochemistry, Organic Chemistry, Drug Discovery. Subjects:. Science , ... A new method of adding fluorescent labels to the middle of oligonucleotides is reported. Diets-Alder cycloaddition was used to ... This is a new approach to internal oligonucleotide chemistry that opens Lip a large range of possibilities for further ...https://strathprints.strath.ac.uk/1064/
Sensitive and specific detection of microRNAs by northern blot analysis using LNA-modified oligonucleotide probes
Synthesis of the LNA-modified oligonucleotide probes. The LNA-modified and DNA oligonucleotide probes (Table (Table1)1) were ... Comparison of LNA2- and LNA3-modified oligonucleotide probes with DNA probes in the detection of the low-abundant miR171 in A. ... Besides being highly efficient as northern probes, the same LNA-modified oligonucleotide probes would also be useful for miRNA ... Besides being highly efficient as northern probes, the same LNA3-modified oligonucleotide probes could also be useful for ...http://pubmedcentralcanada.ca/pmcc/articles/PMC545470/?lang=en-ca
Hybridization of Oligonucleotide Probes in Aqueous Solutions: Washing in Buffers Containing Quaternary Ammonium Salts
Synthesis of cDNA Probes from mRNA Using Random Oligonucleotide Primers *Protocol 7: Radiolabeling of Subtracted cDNA Probes by ... Hybridization of Oligonucleotide Probes in Aqueous Solutions: Washing in Buffers Containing Quaternary Ammonium Salts. ( ... TMACl is used with probes that are 1450 nucleotides in length, whereas TEACl is used with oligonucleotides that are 50200 ... Protocol 18: Hybridization of Oligonucleotide Probes in Aqueous Solutions: Washing in Buffers Containing Quaternary Ammonium ...http://molecularcloning.com/index.php?prt=160
"Short oligonucleotide probes containing G-stacks display abnormal bind"
These probes are less likely to covary with other probes that interrogate the same genes. Moreover, we found that these probes ... we describe a probe performance assessment method based on the concordance of the observed signals from probes that share ... which gives reasonable estimates of binding affinity for most other probes. These results suggest that probes containing G- ... However, due to lack of tools, the observed microarray data have not been used to assess the performance of individual probes ...http://stars.library.ucf.edu/facultybib2000/7801/
Design and evaluation of 16S rRNA sequence based oligonucleotide probes for the detection and quantification of Comamonas...
We have designed an oligonucleotide probe for use in fluorescent in situ hybridization (FISH) and two pairs of PCR primers ... The FISH probe and one of the PCR primer pairs are suitable for quantification of C. testosteroni in mixed microbial ... The oligonucleotides presented here provide a useful tool to study C. testosteroni population dynamics in mixed microbial ... Probe CteA was labelled with the cyanine dye CY3, whereas the EUB probe mix (an equimolar mixture of probes EUB338, EUB338-II, ...https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-6-54
Use of an oligonucleotide probe to detect Vibrio parahaemolyti...
Use of an oligonucleotide probe to detect Vibrio parahaemolyticus in artificially contaminated oysters.: A 26-mer ... Use of an oligonucleotide probe to detect Vibrio parahaemolyticus in artificially contaminated oysters.. Authors * C Lee ... This oligonucleotide probe specifically reacted with DNA from 89 of 95 V. parahaemolyticus isolates but not with DNA from other ... The probe could be used to directly identify V. parahaemolyticus in artificially contaminated food without an isolation step. ...https://www.mysciencework.com/publication/show/use-of-an-oligonucleotide-probe-to-detect-vibrio-parahaemolyticus-in-artificially-contaminated-oysters
Creating Scientific Software, with Application to Phylogenetics and Oligonucleotide Probe Design
The question of what makes a good microarray probe was a research area at the time, and YODA was developed to incorporate the ... YODA is an application primarily for selecting microarray probe sequences for measuring gene expression. At the time of its ... development, none of the existing programs for this task satisfied the best-known requirements for microarray probe selection. ...https://vtechworks.lib.vt.edu/handle/10919/64366
Fluorescence quenching of gold nanoparticles integrating with a conformation-switched hairpin oligonucleotide probe for...
Fluorescence quenching of gold nanoparticles integrating with a conformation-switched hairpin oligonucleotide probe for ...https://www.growkudos.com/publications/10.1039%25252Fc2cc35564g/reader
Design and application of group-specific oligonucleotide probes for detecting and monitoring mouse clostridia. - Semantic...
The oligonucleotide probe set including our newly developed and previously described probes used in this study can be applied ... In this study, five new oligonucleotide probes were designed and applied to detect these clostridial groups that are essential ... These five new probes for mouse clostridia were able to detect the difference in clostridial diversity in each mouse group. In ... pathogen-free mice from different breeding colonies were analysed by fluorescence in situ hybridization using these five probes ...https://www.semanticscholar.org/paper/Design-and-application-of-group-specific-oligonucl-Momose-Park/6cf9689e9211ec5c5389453c7f9f8e8f6a3017d1
BIOLOGICAL SAMPLE TARGET CLASSIFICATION, DETECTION AND SELECTION METHODS, AND RELATED ARRAYS AND OLIGONUCLEOTIDE PROBES -...
... two oligonucleotide probes in combination with other probes, and in particular three or more oligonucleotide probes herein ... the oligonucleotide probe being in combination with at least four other oligonucleotide probes, wherein: the oligonucleotide ... the oligonucleotide probe being in combination with at least four other oligonucleotide probes, wherein: the oligonucleotide ... The oligonucleotide probes can be included in one or more compositions, and each oligonucleotide probe can be in a composition ...http://www.patentsencyclopedia.com/app/20130267429
Biosensors | Free Full-Text | Thiol- and Biotin-Labeled Probes for Oligonucleotide Quartz Crystal Microbalance Biosensors of...
Grafting on a gold surface of 20-base thiol- or biotin-labeled probe, and selective hybridization with the complementary 20- ... Hybridization kinetic curves indicate that the biosensor designed with the thiol-labeled probe is more sensitive, and that the ... biosensor designed with the biotin-labeled probe has a shorter time response and a higher hybridization efficiency. ... Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been ...http://mdpi.com/2079-6374/2/3/245
Faculty Collaboration Database - Assessing unmodified 70-mer oligonucleotide probe performance on glass-slide microarrays. ...
Oligonucleotide Array Sequence Analysis. Oligonucleotide Probes. Polymerase Chain Reaction. Sensitivity and Specificity. View ... Assessing unmodified 70-mer oligonucleotide probe performance on glass-slide microarrays. Genome Biol 2003;4(1):R5 PMID: ... The correlation coefficient between oligonucleotide and cDNA probes for identifying differentially expressed genes was 0.80, ... BACKGROUND: Long oligonucleotide microarrays are potentially more cost- and management-efficient than cDNA microarrays, but ...https://fcd.mcw.edu/?module=search&func=showPublication&id=28255
SINGLE-USE TEST MODULE FOR DETECTION OF HYBRIDIZATION OF TARGETS WITH OLIGONUCLEOTIDE PROBES - diagram, schematic, and...
Back to SINGLE-USE TEST MODULE FOR DETECTION OF HYBRIDIZATION OF TARGETS WITH OLIGONUCLEOTIDE PROBES , All Patents . ... SINGLE-USE TEST MODULE FOR DETECTION OF HYBRIDIZATION OF TARGETS WITH OLIGONUCLEOTIDE PROBES - diagram, schematic, and image 93 ...http://www.patentsencyclopedia.com/imgfull/20110312810_93
The ¤development of oligonucleotide probes and PCR methods specific for Salmonella or for Salmonella typhimurium and...
The ¤development of oligonucleotide probes and PCR methods specific for Salmonella or for Salmonella typhimurium and ...http://www.forskningsdatabasen.dk/catalog/2192957953
Radiolabeling of Subtracted cDNA Probes by Random Oligonucleotide Extension
Synthesis of cDNA Probes from mRNA Using Random Oligonucleotide Primers *Protocol 7: Radiolabeling of Subtracted cDNA Probes by ... Radiolabeling of Subtracted cDNA Probes by Random Oligonucleotide Extension. (Protocol summary only for purposes of this ... Protocol 18: Hybridization of Oligonucleotide Probes in Aqueous Solutions: Washing in Buffers Containing Quaternary Ammonium ... Protocol 3: Labeling of DNA Probes by Nick Translation *Protocol 4: Labeling of DNA Probes by Polymerase Chain Reaction * ...http://molecularcloning.com/index.php?prt=149
Aptamer-Based Electrochemical Sensors with Aptamer−Complementary DNA Oligonucleotides as Probe
"Label-free luminescent oligonucleotide-based probes" by Dik-Lung Ma, Hong-Zhang He et al.
Label-free DNA-based probes harness the selective interaction between luminescent dyes and functional oligonucleotides that ... DNA oligonucleotides represent popular tools for the development of sensing platforms due to their low cost, rich structural ... Based on the numerous examples of label-free luminescent DNA-based probes reported recently, we envisage that this field would ... The label-free strategy aims to overcome some of the drawbacks associated with the use of covalently-labeled oligonucleotides ...https://repository.hkbu.edu.hk/hkbu_staff_publication/585/
CBFα3 (AML2) Is Induced by TGF-β1 to Bind and Activate the Mouse Germline Ig α Promoter | The Journal of Immunology
Oligonucleotide probes for EMSAs. Double-stranded (ds) oligonucleotides were prepared and labeled as previously described (51). ... The same blot was probed in A and B. C, EMSAs in which the TβRE probe was incubated with 2 μg of NEs from splenic B (Spl B), I. ... To further examine the importance of the nucleotides mutated in mutant m1, we used the m1-TβRE oligonucleotide as a probe in ... Oligonucleotides. The sequences of the top strand of the double-stranded oligonucleotides used in EMSAs are given below. Lower ...http://www.jimmunol.org/content/161/12/6751
Complete Northern Systems | Thermo Fisher Scientific
Optimized Hybridization for Oligonucleotide Probes ULTRAhyb™-Oligo Hybridization Buffer is optimized specifically for ... Figure 3 shows identical Northern blots that were hybridized overnight with a 32P-labeled 44-mer oligonucleotide probe to ‹- ... Oligonucleotides ranging in length from 26 nt to 45 nt have been tested. ULTRAhyb-Oligo works best using oligonucleotides with ... Each blot was hybridized with DNA probe to p53 at a concentration of 1 x 10 6 cpm probe/ml hybridization solution. Incubation ...https://www.thermofisher.com/mx/en/home/references/ambion-tech-support/northern-analysis/tech-notes/premade-rnase-free-northern-reagents-.html
Genomic Analysis Using High-Density Single Nucleotide Polymorphism-Based Oligonucleotide Arrays and Multiplex Ligation...
Molecular Probes). Probes for the Ewing's sarcoma region in 22q12 were used simultaneously with the probe for INI1 as an ... 8 This kit contains 2 probes for each of the 9 exons of INI1, probes for 9 other genes on chromosome 22, and 14 control probes ... High-density SNP-based oligonucleotide array analysis. The high-density oligonucleotide array analysis was done using an ... The probes were applied to slides of the tumor cells and codenatured at 75°C on an Isotemp 125D heat block (Fisher Scientific ...http://clincancerres.aacrjournals.org/content/15/6/1923
RANDOX 25I-NBOMe (2C-I-NBOMe, Cimbi-5) ELISA [R-NBOMe] Equl offers Enzyme Assay Kits, Reagent Mixtures, Enzymes, Glycobiology,...
SD E-Chem Testers and Replacement Probes. * Electrochemistry Calibration Solutions. * BD 600 System Accessories ...http://www.equl.com/index.php?main_page=product_info&cPath=70_1541_1709&products_id=322614
No symbol available for ENSRNOG00000038982 - ddPCR Probe - Digital PCR | PrimePCR | Bio-Rad
ddPCR™ probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled ... PrimePCR™ ddPCR™ Expression Probe Assay: No symbol available for ENSRNOG00000038982, Rat. print ... PrimePCR™ ddPCR™ Gene Expression Probe Assays Product Insert, Rev A. Click to download ... Info: FAM; Same primer pair and probe as used in qPCR assay qRnoCEP0047500; Exonic ...http://www.bio-rad.com/en-us/prime-pcr-assays/assay/drnocpe5200395-primepcr-ddpcr-expression-probe-assay-no-symbol-available-for-ensrnog00000038982-rat