Arginase Promotes Neointima Formation in Rat Injured Carotid Arteries | Arteriosclerosis, Thrombosis, and Vascular Biology
... and harvested 2 weeks after injury (magnification 100×). B, Morphometric analysis of neointima formation 2 weeks postinjury. ... peaked after 1 week, and remained elevated 2 weeks after balloon injury. The peak increase in arginase activity observed 1 week ... Animals treated with empty gel exhibited a significant and concentric neointima. In contrast, a markedly diminished neointima ... Arginase Promotes Neointima Formation in Rat Injured Carotid Arteries. Kelly J. Peyton, Diana Ensenat, Mohammed A. Azam, Amit N ...http://atvb.ahajournals.org/content/29/4/488
Anti-MCP-1 Gene Therapy Inhibits Vascular Smooth Muscle Cells Proliferation and Attenuates Vein Graft Thickening Both In Vitro...
In all samples a neointima formed within 4 weeks of culturing. However, quantification revealed reduced neointima formation in ... After 2 weeks the expression in the IH decreased, and it was scarcely detected after 4 weeks (Figure 1A). ... Nature and origin of the neointima in whole vessel wall organ culture of the human saphenous vein. Virchows Arch. 1996; 428: 59 ... In HSV cultures neointima formation was inhibited by 53%. In addition, we demonstrate a direct inhibitory effect of 7ND-MCP-1 ...http://atvb.ahajournals.org/content/26/9/2063
Increased Secretion of Tissue Inhibitors of Metalloproteinases 1 and 2 From the Aortas of Cholesterol Fed Rabbits Partially...
... a significant increase in TIMP-1 expression was observed in rabbit aortic neointima 8 weeks after selective ... Male New Zealand White rabbits (6 weeks old initially) were fed a diet containing 1% cholesterol during 8 weeks according to an ... Arrows highlight an area of the deep neointima showing diminished and lacunar gelatinase activity. Scale bar, 100 μm. Results ... Adenovirus mediated gene transfer of the human TIMP-1 gene inhibits smooth muscle cell migration and neointima formation in ...http://atvb.ahajournals.org/content/19/7/1700
Kallikrein Gene Delivery Inhibits Vascular Smooth Muscle Cell Growth and Neointima Formation in the Rat Artery After Balloon...
1 week after angioplasty and completed neointima formation at 2 weeks. Decreased endogenous tissue kallikrein levels during ... Two weeks after gene delivery, rats were anesthetized and the left and right carotid artery were removed and embedded in ... Role of kinins and nitric oxide in the effects of ACE inhibition on neointima formation. Circ Res. 1993;72:1202-1210. ... Inhibition of neointima formation in rat carotid artery after adenovirus-mediated kallikrein gene delivery. A through D show ...http://hyper.ahajournals.org/content/34/2/164
Histone Deacetylase 3 Is Critical in Endothelial Survival and Atherosclerosis Development in Response to Disturbed Flow |...
Neointima formation on the grafts was checked 3 weeks later. Bar=100 μm. C, Mean±SD of neointima area from each group is ... To evaluate the role of HDAC3 in neointima formation, the isografts were harvested 3 weeks later. The isografts infected with ... Three weeks later, the grafts were harvested and fixed with formalin. The sections were stained with hematoxylin and eosin. ... Robust Neointima Formation in Aortic Isografts After HDAC3 Knockdown in Endothelial Cells. To investigate whether HDAC3 is ...http://circ.ahajournals.org/content/121/1/132
Tranilast Suppresses Vascular Chymase Expression and Neointima Formation in Balloon-Injured Dog Carotid Artery | Circulation
... or vehicle was orally administered to beagles for 2 weeks before and 4 weeks after balloon injury. Four weeks after the injury ... The balloon injury elicited remarkable neointima formation (Figure 1A⇓). Tranilast markedly prevented this neointima formation ... A indicates adventitia; M, media; N, neointima. Immunohistochemical labeling for PCNA in neointima of injured common carotid ... for 2 weeks before balloon injury and for 4 weeks thereafter throughout the experimental period. The other group (n=7) received ...http://circ.ahajournals.org/content/99/8/1084
US20040002755A1 - Method and apparatus for treating vulnerable coronary plaques using drug-eluting stents - Google...
By delaying the release of the anti-restenosis agent, a thin layer of neointima will grow during the preset period. This tissue ... In a preferred embodiment the release delay period of two (2) weeks should allow sufficient neointima tissue growth. ... leading to undesirable tissue growth in the form of neointima and/or intimal hyperplasia. If left untreated, this neointima and ... The thin layer of the neointima 600 remaining is sufficient to seal over and cover the vulnerable plaque 315, and provide ...https://patents.google.com/patent/US20040002755
Dose-Response Effects of 32P Radioactive Stents in an Atherosclerotic Porcine Coronary Model | Circulation
... reported a reduction in neointima after 12 weeks in rabbit iliac arteries after placement of radioactive stents with only 13 ... Neointima of nonradioactive stents consists of well-organized SMCs within a collagen matrix. Neointima of 0.5-μCi 32P ... The neointima of the radioactive stents with ≤1.0 μCi of 32P appeared similar to that of the nonradioactive stents (Figure 2⇓ ... The neointima of the stents with ≥3.0 μCi 32P was composed of SMCs and a proteoglycan matrix with calcification, foam cells, ...http://circ.ahajournals.org/content/100/14/1548
CREB-Mediated IL-6 Expression Is Required for 15(S)-Hydroxyeicosatetraenoic Acid-Induced Vascular Smooth Muscle Cell Migration ...
HETE-CREB signaling in balloon injury-induced neointima formation, 2 weeks after balloon injury and transduction with Ad-GFP, ... Smooth muscle cell migration from media to intima is a key event in neointima formation.3 To validate the in vitro observations ... Conclusions- The above results suggest a role for 15-LOX2-15-HETE in the regulation of VSMC migration and neointima formation ... C, All the conditions were the same as in panel B except that 2 weeks after balloon injury arteries isolated, fixed, cross- ...http://atvb.ahajournals.org/content/29/6/809
Application # 2017/0340432. Bioerodible Wraps and Uses Therefor - Patents.com
... hyperplastic neointima during the first week after injury resulting from balloon angioplasty. In addition, AVGs that undergo ... As above, the matrix typically bioerodes in situ over a time period ranging from 12 hours to two weeks.  In one ... In a typical application, the matrix biodegrades over a period of two weeks to two years. In a preferred embodiment, the matrix ... The matrix of the device typically bioerodes in situ (when implanted) over a time period ranging from 12 hours to two weeks, ...http://patents.com/us-20170340432.html
Abstract 710: The Adipokine Leptin Promotes Integrin-Mediated Adhesion of Endothelial Progenitor Cells | Circulation
Morphometric analysis 3 weeks after injury revealed a significant decrease in neointima formation in mice injected with leptin- ...http://circ.ahajournals.org/content/114/Suppl_18/II_121.1
VEGF Blockade Inhibits Lymphocyte Recruitment and Ameliorates Immune-Mediated Vascular RemodelingNovelty and Significance |...
Bevacizumab (5 mg/kg IP, 3 times a week) significantly reduced neointima (0.74±0.07 mm2 and 0.52±0.09 mm2, respectively, for ... Animals were euthanized at 4 weeks after PBMC transfer or adenovirus injection (5 weeks after coronary artery transplantation ... led to significant neointima formation and expansive remodeling over a period of 4 weeks, as previously described.11 The intima ... or 4 weeks after, human PBMC transfer, demonstrating VEGF and VEGFR-1, both predominantly expressed in the media and neointima ...http://circres.ahajournals.org/content/107/3/408
Critical Role of Mst1 in Vascular Remodeling After Injury | Arteriosclerosis, Thrombosis, and Vascular Biology
... days to weeks). VSMC accumulation in the neointima of injured rat carotid arteries reached a maximal level at 2 weeks after ... Shibata R, Kai H, Seki Y, Kato S, Morimatsu M, Kaibuchi K, Imaizumi T. Role of Rho-associated kinase in neointima formation ... It was reported that both proliferation and apoptosis were increased in the neointima of the balloon-injured rat carotid artery ... β-galactosidase activity was observed in both the neointima and media in the AdLacZ-infected artery (data not shown). ...http://atvb.ahajournals.org/content/25/9/1871
After a 4-week period to allow formation of a neointima, animals were killed by intravenous overdose of pentobarbital and both ... neointima 0.035 ± 0.007 mol glucose/mg protein, n = 12, P , 0.01) (Fig. 1D). Considering the well-defined role of GLUT1 in ... neointima 5,370 ± 720 mean arbitrary units, n = 4, P , 0.03). The increase in GLUT1 and GLUT4 protein expression was ... neointima 3,546 ± 615 mean arbitrary units, n = 4, P , 0.04). In addition, GLUT4 protein expression was also significantly ...http://diabetes.diabetesjournals.org/content/50/5/1171
Lysophosphatidic Acid Receptors 1 and 2 Play Roles in Regulation of Vascular Injury Responses but Not Blood Pressure |...
Four weeks after carotid ligation, neointima developed along the length of the carotid in WT mice (Figure 2A and 2B). The ... Unlike either the LPA2−/− or LPA1−/− mice, which displayed normal and enhanced neointima, respectively, LPA1−/−2−/− double ... A, Representative combined Masson elastin-stained sections of carotid arteries at 4 weeks after surgery taken ≈1.2 mm from the ... Neointimal formation along the length of the vessel was assessed at 4 weeks after surgery using computer assisted morphometry ...http://circres.ahajournals.org/content/103/6/662
Alternatively Spliced Tissue Factor Promotes Plaque Angiogenesis Through the Activation of Hypoxia-Inducible Factor-1α and...
As expected, 4 weeks after arterial wire injury, histological analysis revealed the presence of complex neointima lesions in ... ApoE−/− mice (8-week-old mice on a C57BL/6 background) were fed a Western-type diet (Harlan Laboratories) beginning 2 weeks ... Eight-week-old male C57Bl6 mice (The Jackson Laboratory, Bar Harbor, ME) were used for the in vivo Matrigel Plug model. ApoE−/− ... asTF Overexpression Promotes Neointima Formation and Angiogenesis in Response to Arterial Injury in ApoE−/− Mice. To test the ...http://circ.ahajournals.org/content/130/15/1274
Effect of Selective or Combined Inhibition of Integrins αIIbβ3 and αvβ3 on Thrombosis and Neointima After Oversized Porcine...
... started before and continued for 2 weeks after porcine PTCA, significantly reduce neointima formation at 28 days. αIIbβ3 ... processes thought to be central to neointima formation,4 and antagonists of these mitogens reduce neointima formation.5 6 The ... αvβ3 antagonism has been shown to reduce neointima in other studies,13 14 15 17 although agent specificity has varied, and ... The reduction in neointima with αIIbβ3 antagonists appears to be related to a reduction in thrombus formation at the site of ...http://circ.ahajournals.org/content/103/8/1135
Abstract 20944: Perivascular Progenitor Cells Contribute To Neointima Formation After Wire-induced Injury | Circulation
18.98 at 2 weeks to only 5.31±2.21 at 16 weeks after dilation. Whereas very few EGFP+-cells co-expressed α-smooth muscle actin ... Abstract 20944: Perivascular Progenitor Cells Contribute To Neointima Formation After Wire-induced Injury. Jan-Marcus Daniel, ... In conclusion, we provide evidence that BMPCs within the neointima rarely co-express markers for vascular cells and do not ... Most of the accumulating EGFP+-cells were identified as inflammatory cells (CD45, CD68), and their number in the neointima ...http://circ.ahajournals.org/content/122/Suppl_21/A20944
Time courses of apoptosis and cell proliferation and their relationship to arterial remodeling and restenosis after angioplasty...
Scattergrams of apoptosis in the neointima (A and C)and media (B and D)versus restenosis (A and B)and arterial remodeling (C ... Four weeks later, angioplasty was performed in 40 femoral arteries. Eight arteries in four animals did not undergo angioplasty ... A)Macrophages (brown)revealed by monoclonal anti-rabbit macrophage (RAM11). (B)Smooth muscle cells (brown)in the neointima and ... In the neointima and media, profiles of cell proliferation and apoptosis were similar, but the peak in cell proliferation ...http://www.onlinejacc.org/content/39/10/1680
Matrix Metalloproteinase-9 Is Necessary for the Regulation of Smooth Muscle Cell Replication and Migration After Arterial...
SMCs were present in the intima by 6 days after injury, and 4 weeks later, a marked thickening of the neointima was observed in ... Figure 3. Intimal and medial SMC density (A) and area (B) in WT (solid bar) and KO (hatched bar) carotid arteries at 4 weeks ... Furthermore, the neointima is significantly smaller in MMP-9−/− arteries 28 days after injury. These data confirm our ... In this study, we sought to determine whether MMP-9 is critical for SMC migration and for the formation of a neointima by using ...http://circres.ahajournals.org/content/91/9/845.full
Abstract 14668: Epac1 Deficiency Inhibit Neointima Formation After Vascular Injuryin vivo | Circulation
Four weeks after injury, the area of intimal thickening, and internal lumen were evaluated by Elastica van Gieson's staing. ... Abstract 14668: Epac1 Deficiency Inhibit Neointima Formation After Vascular Injuryin vivo. Yuko Kato, Utako Yokoyama, Satoshi ... Conclusion:These results suggest that Epac1 deficiency inhibit neointima formation after vascular injury in vivo. ... Abstract 14668: Epac1 Deficiency Inhibit Neointima Formation After Vascular Injuryin vivo ...http://circ.ahajournals.org/content/124/Suppl_21/A14668
Possible Antiatherosclerotic Effects of Hot Tubs
Four weeks after the placement of the cuffs, neointima was formed in the subendothelial layers of the arteries in the control ... rats chosen for this study1 were given 15 minutes of hot water baths daily for 4 weeks to study the effects of heat shock on ...http://www.life-enhancement.com/magazine/article/998-possible-antiatherosclerotic-effects-of-hot-tubs
Patent US5997468 - Intraluminal drug eluting prosthesis method - Google Patents
This usually occurs in about two weeks. The drug elution rate does not need to be uniform, and may be tailored to fit the need ... Damage causes healing with deposition of platelets, fibrin formation and proliferation of neointima 37 which as shown in FIG. 7 ... In the case of stents, the prosthesis materials will maintain vessel support for at least two weeks or until incorporated into ... This results in limited but quick neointima formation 40 and intimal proliferation over individual stent filaments progressing ...http://www.google.com/patents/US5997468?dq=5191154
Graft-Extrinsic Cells Predominate in Vein Graft Arterialization | Arteriosclerosis, Thrombosis, and Vascular Biology
... compared with the neointima taken as a whole (Figure 3E). Thus, focal cellular vein graft wall expansions observed in 2-week- ... steady-state vein graft wall thickness is reached only by 4 weeks postoperatively in this animal model.) In these 2-week-old ... D-G, Six-week-old WT IVC-to-βgal and βgal IVC-to-WT specimens were processed as in Figure 1, with the omission of eosin ... A-C, Two-week-old vein grafts (n=3) were formalin-fixed, paraffin-embedded, sectioned at 200-μm intervals, and stained either ...http://atvb.ahajournals.org/content/24/3/470
Blockade of the Intermediate-Conductance Calcium-Activated Potassium Channel as a New Therapeutic Strategy for Restenosis |...
Reduced neointima formation in TRAM-34-treated animals resulted in larger residual lumina at week 2 (+34%; P,0.05) and at week ... 5 g at week 2; 99±6 g at week 6), similar to the vehicle-treated group (25±4 g at week 2, 90±15 g at week 6). In contrast, the ... at week 2 (−9%; P,0.01) and week 6 (−19%; P,0.01) than vehicle-treated control animals (−36% at week 2 and −50% week 6). Less ... 6 Although neointima formation progressively increased from week 1 to week 6 after BCI in the vehicle-treated group, the area ...http://circ.ahajournals.org/content/108/9/1119