*  US8431799B1 - Soybean cultivar 12245180 - Google Patents
... random amplified polymorphic DNA), 3 classical markers, and 4 isozyme loci. See also, Shoemaker, R. C., 1994 RFLP Map of ... Mutation Breeding. Mutation breeding is another method of introducing new traits into soybean cultivar 12245180. Mutations that ... The goal of artificial mutagenesis is to increase the rate of mutation for a desired characteristic. Mutation rates can be ... Both naturally occurring and transgenic DNA sequences may be introduced through backcrossing techniques. A backcross conversion ...
  https://patents.google.com/patent/US8431799B1/en
*  Soybean Cultivar 86351435 - Patent application
... random amplified polymorphic DNA), 3 classical markers, and 4 isozyme loci. See also, Shoemaker, R. C., 1994 RFLP Map of ... Mutation Breeding [0202]Mutation breeding is another method of introducing new traits into soybean variety 86351435. Mutations ... The goal of artificial mutagenesis is to increase the rate of mutation for a desired characteristic. Mutation rates can be ... Various molecular marker techniques may be used in combination to enhance overall resolution. [0206]Soybean DNA molecular ...
  http://www.patentsencyclopedia.com/app/20110055949
*  Soybean Variety S06-4649RR - Patent application
... random amplified polymorphic DNA), three classical markers, and four isozyme loci. See also, Shoemaker R. C. 1994 RFLP Map of ... The goal of artificial mutagenesis is to increase the rate of mutation for a desired characteristic. Mutation rates can be ... Method to develop high oleic acid soybeans using conventional soybean breeding techniques. ... The development of soybean sublines and the use of accelerated yield technology is a plant breeding technique. Using S06-4649RR ...
  http://www.patentsencyclopedia.com/app/20130232637
*  Soybean Variety XB23K11 - Patent application
... random amplified polymorphic DNA), three classical markers, and four isozyme loci. See also, Shoemaker "RFLP Map of Soybean". ... The goal of artificial mutagenesis is to increase the rate of mutation for a desired characteristic. Mutation rates can be ... 7. A method for developing a second soybean plant comprising applying plant breeding techniques to a first soybean plant, or ... The development of soybean sublines and the use of accelerated yield technology is a plant breeding technique. [0180] Soybean ...
  http://www.patentsencyclopedia.com/app/20120240259
*  Preimplantation genetic diagnosis guided by single-cell genomics | Springer for Research & Development
The method was able to pinpoint recombination sites between parental homologous chromosomes and inherited DNA mutations could ... Wang J, Fan HC, Behr B, Quake SR: Genome-wide single-cell analysis of recombination activity and de novo mutation rates in ... In these assays, preferably the causal genetic mutation, along with a minimum of two linked polymorphic markers, are genotyped ... by NGS one can interrogate almost every nucleotide amplified by the WGA method, whereas microarrays examine only certain loci ...
  https://rd.springer.com/article/10.1186/gm475
*  SNP Markers and Their Impact on Plant Breeding
PCR-based markers that include random amplification of polymorphic DNA (RAPD) [7], amplified fragment length polymorphism (AFLP ... P. Vos, R. Hogers, M. Bleeker et al., "AFLP: a new technique for DNA fingerprinting," Nucleic Acids Research, vol. 23, no. 21, ... In a study conducted in canola to map the fad2 and fad3 gene, single nucleotide mutations were identified by sequencing the ... However, in organisms that do not have a reference genome, GBS-derived SNPs must be validated using one of the techniques that ...
  https://www.hindawi.com/journals/ijpg/2012/728398/
*  Protocols and Video Articles Authored by Atsuo Ogura (Translated to Portuguese)
... is a method for differential subtraction of polymorphic (RFLP) DNA fragments between two DNA samples of interest without probes ... is a widely used assisted reproductive technique, the fertilization rates and pregnancy rates of immature spermatids especially ... By using an amplified in situ hybridization technique, we detected and counted the renal EPO-producing cells under both ... the success rate for producing live offspring by cloning remains < 5%. Nevertheless, the techniques have potential as important ...
  https://www.jove.com/author/Atsuo_Ogura?language=Portuguese
*  Molecular-weight size marker - Wikipedia
RAPD Random amplified polymorphic DNA is a technique that is conducted similar to AFLP. The difference is that the molecular ... Microsatellites have a high and complex mutation rate, which is their major disadvantage. AFLP Amplified fragment length ... One such method employs the technique of partial ligation. DNA ligation is the process by which linear DNA pieces are connected ... DNA sequence polymorphism uses older techniques like RFLP, but on a larger scale. Sequencing is much faster and more efficient ...
  https://en.wikipedia.org/wiki/Molecular-weight_size_marker
*  Protocols and Video Articles Authored by Lihua Zhang
Mitochondrial DNA of velvet antler was amplified with random amplified polymorphic DNA (RAPD) technique and the PCR products ... If left un-repaired in vivo by the specific enzyme, this nanocavity will result in nucleotide mutation in the following DNA ... It can be found that the technique of RAPD combined with HPCE is advantageous in rich polymorphism, high detection rate, simple ... DNA and genomic DNA were extracted from Cervi Cornu Pantotrichum through the modified alkaline and the salt-extracting method ...
  https://www.jove.com/author/Lihua_Zhang
*  Microsatellite allele dose and configuration establishment (MADCE): an integrated approach for genetic studies in...
The MADCE method therefore enables researchers to tackle many of the genotyping problems that arise when performing mapping, ... These microsatellites amplified 35 different alleles based on size. Using MADCE, we uncovered 30 highly informative segregating ... More importantly, it is the first technique to allow the determination of the full allelic configuration in pedigreed breeding ... The method, named Microsatellite Allele Dose and Configuration Establishment (MADCE), can be applied to mapping populations and ...
  https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-12-25
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... without prior DNA extraction. In the model demonstrated here, an example of derived cleaved amplified polymorphic sequence ... First, the de novo mutation rate in humans is relatively high, so new mutations are generated at a high frequency in the ... "Eberwine method"3, microarray analysis4 and for RNA vaccine studies5. The technique can also be used for producing radiolabeled ... Cross-validation within the derivation set can be performed using bootstrap resampling techniques9. Forward validation is ...
  https://www.jove.com/visualize/abstract/20593030/human-intelligence-polymorphisms-dna-methyltransferase-genes-involved
*  Human mitochondrial molecular clock - Wikipedia
Estimates of the mutation rate of human mitochondrial DNA (mtDNA) vary greatly depending on the available data and the method ... Random genetic drift may also cause the loss of mutations. For these reasons, the actual mutation rate will not be equivalent ... The Hypervariable regions are known to be highly polymorphic relative to other parts of the genome. The rate at which mutations ... Using these techniques this group came up with a TMRCA of 140,000 to 290,000 years. It should be noted however that Cann et al ...
  https://en.wikipedia.org/wiki/Human_mitochondrial_molecular_clock
*  Microsatellite - Wikipedia
They have a higher mutation rate than other areas of DNA leading to high genetic diversity. Microsatellites are often referred ... then amplifying specific polymorphic regions of the extracted DNA by means of the polymerase chain reaction. Once these ... Random microsatellite primers can be developed by cloning random segments of DNA from the focal species. These random segments ... This method of RNA splicing is believed to have diverged from human evolution at the formation of tetrapods and to represent an ...
  https://en.wikipedia.org/wiki/Microsatellite
*  Blood tests facts, information, pictures | Encyclopedia.com articles about Blood tests
... a technique that can amplify large amounts of specific small sequences of DNA from the human genome. It is also due to the ... DNA Profiling Genetics Copyright Genetics Society of America. DNA Profiling. DNA profiling is a molecular testing method used ... Laboratory DNA Science: An Introduction to Recombinant DNA Techniques and Methods of Genome Analysis. Menlo Park, CA: Addison- ... DNA molecular analysis has also been used in the diagnosis of clinical disorders. Many genetic diseases are caused by mutations ...
  https://www.encyclopedia.com/medicine/divisions-diagnostics-and-procedures/medicine/blood-tests
*  Endotoxin-tolerant Mice Have Mutations in Toll-like Receptor 4 (Tlr4) | JEM
... flanking the region predicted to contain the C3H/HeJ mutation were used to amplify genomic DNA from our two panels of ... dATP by the random-primer method ((23)). Filters were washed for 30 min at 65°C in 0.5× SSC and 0.1% SDS and exposed for 4-16 h ... 1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6-13, ... Using high-resolution genetic mapping techniques, we have restricted the position of the Lps gene to a 0.9-cM region of ...
  http://jem.rupress.org/content/189/4/615
*  Multiple displacement amplification - Wikipedia
... is a non-PCR based DNA amplification technique. This method can rapidly amplify minute amounts of DNA samples to a reasonable ... ADO appears to be independent of the fragment sizes and has been reported to have similar rate in other single-cell techniques ... The large size of MDA-amplified DNA products also provides desirable sample quality for identifying the size of polymorphic ... The amplification bias is currently observed to be random. It might affect the analysis of small stretches of genomic DNA in ...
  https://en.wikipedia.org/wiki/Multiple_displacement_amplification
*  Preimplantation genetic diagnosis guided by single-cell genomics | Genome Medicine | Full Text
Here, we discuss the latest single-cell genomics methodologies based on DNA microarrays, single-nucleotide polymorphism arrays ... The method was able to pinpoint recombination sites between parental homologous chromosomes and inherited DNA mutations could ... Wang J, Fan HC, Behr B, Quake SR: Genome-wide single-cell analysis of recombination activity and de novo mutation rates in ... In these assays, preferably the causal genetic mutation, along with a minimum of two linked polymorphic markers, are genotyped ...
  https://0-genomemedicine-biomedcentral-com.brum.beds.ac.uk/articles/10.1186/gm475
*  Collection of Genomic DNA from Adults in Epidemiological Studies by Buccal Cytobrush and Mouthwash | Cancer Epidemiology,...
... and PCR success rates to amplify fragments of the β-globin gene from phase 1 of the study by method of collection and DNA ... Other DNA Extraction Techniques. In this section, several DNA extraction techniques are compared with phenol-chloroform ... and screening for mutations in the BRCA1/2 genes in a subset of phenol-chloroform DNA extracts. Relative to DNA extracted by ... Both polymorphic and wild-type samples were identified by dHPLC, and they were subsequently confirmed by sequence analysis. ...
  http://cebp.aacrjournals.org/content/10/6/687
*  Preimplantation genetic diagnosis - Wikipedia
The FISH technique is considered to have an error rate between 5 and 10%. The main problem of the use of FISH to study the ... The PGD allows studying the DNA of eggs or embryos to select those that carry certain mutations for genetic diseases. It is ... Recently a method was developed allowing to fix metaphase plates from single blastomeres. This technique in conjunction with ... Because the Y chromosome region Kontogianni was amplifying contained many repeats, it was more efficient than trying to amplify ...
  https://en.wikipedia.org/wiki/Preimplantation_genetic_diagnosis
*  Polymorphism (biology) - Wikipedia
Because the heterozygote survives, so does the HgbS allele survive at a rate much higher than the mutation rate (see and refs ... Using Painter's technique they studied the polytene chromosomes and discovered that the wild populations were polymorphic for ... Territories of type A and type B males are random if not mated but alternate if mated: no two breeding males of the same song ... Data is available from 1939 to the present day, got by the usual field method of capture-mark-release-recapture and by genetic ...
  https://en.wikipedia.org/wiki/Polymorphism_(biology)