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*  Identification of an active site cysteine residue in human type I Ins(1,4,5)P3 5-phosphatase by chemical modification and site...
Chemical modification using thiol-directed agents and site-directed mutagenesis have been used to investigate the crucial role ... Furthermore two mutant enzymes were obtained by site-directed mutagenesis of the cysteine residue to serine and alanine ... P3 5-phosphatase by chemical modification and site-directed mutagenesis. David COMMUNI, Christophe ERNEUX ... P3 5-phosphatase by chemical modification and site-directed mutagenesis Message Subject (Your Name) has forwarded a page to you ...
  http://www.biochemj.org/content/320/1/181
*  Site-directed mutagenesis - Wikipedia
Nobel Lecture on Invention of Site-Directed Mutagenesis OpenWetWare Diagram summarizing site-directed mutagenesis. ... Direct gene deletion and site-specific mutagenesis with PCR and one recyclable marker Direct gene deletion and site-specific ... Also called site-specific mutagenesis or oligonucleotide-directed mutagenesis, it is used for investigating the structure and ... "SDM-Assist software to design site-directed mutagenesis primers introducing "silent" restriction sites". BMC Bioinformatics. 14 ...
  https://en.wikipedia.org/wiki/Site-directed_mutagenesis
*  Site directed mutagenesis
... WS via methods%40net.bio.net (by novalidaddress from nurfuerspam.de). Mon Aug 15 03:50:43 EST 2011 * ...
  http://www.bio.net/bionet/mm/methods/2011-August/105220.html
*  help for site directed mutagenesis
... philip hardwidge hardp3 at mayo.edu Fri Feb 23 09:16:01 EST 2001 *Previous message: help ... I would like undertaken site directed mutagenesis, but never did. : , : , Can somebody tell me if it is very difficult to do, ... Several papers from about 10 years ago offer facile PCR-based methods for making site-directed mutants. -Phil Hardwidge Mayo ... Previous message: help for site directed mutagenesis *Next message: help for site directed mutagenesis ...
  http://www.bio.net/bionet/mm/methods/2001-February/087575.html
*  Q5® Site-Directed Mutagenesis Kit | NEB
... site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High- ... The Q5® Site-Directed Mutagenesis Kit enables rapid, ... The Q5 Site-Directed Mutagenesis Kit enables rapid, site- ... DNA Assembly, Cloning and Mutagenesis Kits. Applications: Site Directed Mutagenesis, Site Directed Mutagenesis. * Advantages ... Home DNA Assembly Cloning and Mutagenesis Kits Products Q5® Site-Directed Mutagenesis Kit ...
  https://www.neb.com/products/e0554-q5-site-directed-mutagenesis-kit
*  Site Directed Mutagenesis Of Dienelactone Hydrolase - Digital Library
Site-specific mutants of dienelactone hydrolase were created using mismatched oligonucleotides to prime DNA synthesis. ... Chen, Wei, 1965-. Site Directed Mutagenesis Of Dienelactone Hydrolase, thesis, December 1992; Denton, Texas. (digital.library. ... Site-specific mutants of dienelactone hydrolase were created using mismatched oligonucleotides to prime DNA synthesis. ... Site-specific mutants of dienelactone hydrolase were created using mismatched oligonucleotides to prime DNA synthesis. ...
  https://digital.library.unt.edu/ark:/67531/metadc500900/
*  GeneArt Site-Directed Mutagenesis System - Thermo Fisher Scientific
This system replaces the popular GeneTailor Site-Directed Mutagenesis System, and has been completel ... The GeneArt Site-Directed Mutagenesis System provides a state-of-the-art, simple, convenient, and highly efficient means to ... Site-Directed Mutagenesis System, and has been completely redesigned to be at the leading edge of commercial site-directed ... Optimized Mutagenesis Protocol. The GeneArt® Site-Directed Mutagenesis System has been optimized for efficiency and simplicity ...
  https://www.thermofisher.com/order/catalog/product/A13282
*  Site-Directed Mutagenesis | Thermo Fisher Scientific - CA
... identify enzyme active sites, and design novel proteins in drug discovery. ... Site-directed mutagenesis is a powerful research tool used to study protein function, ... Which site-directed mutagenesis system is right for you?. GeneArt Site-Directed Mutagenesis System GeneArt Site-Directed ... Multisite-directed mutagenesis efficiency. Figure indicates the multisite-directed mutagenesis efficiency of 3 sites of 1 bp or ...
  http://www.thermofisher.com/ca/en/home/life-science/cloning/mutagenesis.html
*  Site-directed mutagenesis-Within 10 bases from Bio S&T
... ,Please email us with project details and we will respond within 24 hours ... Site-directed Mutagenesis from Exon BioSystems. 2. Site-directed DNA Mutagenesis Service from Yorkshire Bioscience Ltd. 3. AMAP ... Date:12/10/2018)... ... December 10, 2018 , ... ... Manufacturing Organization (CDMO) with sites in China and the United States ...
  http://bio-medicine.org/biology-products/Site-directed-mutagenesis-Within-10-bases-from-Bio-S-26T-3832-1/
*  Efficient multi-site-directed mutagenesis directly from genomic template.
... the traditional multi-site-directed mutagenesis method based on overlap extension PCR was improved specifically for complicated ... This method was effectively applied for multi-site-directed mutagenesis directly from mouse genomic DNA, as well as for ... In this article, the traditional multi-site-directed mutagenesis method based on overlap extension PCR was improved ...
  http://www.biomedsearch.com/nih/Efficient-multi-site-directed-mutagenesis/23151786.html
*  Lidstrom:Site-Directed Mutagenesis - OpenWetWare
Lidstrom:Site-Directed Mutagenesis. From OpenWetWare. Revision as of 06:44, 29 February 2012 by Janet B. Matsen. (talk , ... Retrieved from "https://openwetware.org/mediawiki/index.php?title=Lidstrom:Site-Directed_Mutagenesis&oldid=588129" ... Kunkel Mutagenesis. *Justin Siegel at the Baker lab is our local expert. The UW iGem team uses this method. ...
  https://openwetware.org/wiki/?title=Lidstrom:Site-Directed_Mutagenesis&oldid=588129
*  Site-directed mutagenesis-improvements to established methods
... can save you both time and money over some of the common cloning methods used for mutagenesis. ... In vitro site-directed mutagenesis is a core technique in functional genomics studies. Until recently, labs performing site- ... Site-directed mutagenesis techniques have relied primarily on PCR and standard cloning methods. Read about some of the common ... Figure 1. Examples of site-directed mutagenesis by traditional PCR. Primers incorporating the desired base changes are used in ...
  https://www.idtdna.com/pages/education/decoded/article/site-directed-mutagenesis-improvements-to-established-methods
*  Wittrup: Site directed mutagenesis by QuikChange - OpenWetWare
Retrieved from "https://openwetware.org/mediawiki/index.php?title=Wittrup:_Site_directed_mutagenesis_by_QuikChange&oldid=127095 ...
  https://openwetware.org/wiki/Wittrup:_Site_directed_mutagenesis_by_QuikChange
*  Knight:Site-directed mutagenesis/Single site - OpenWetWare
Knight:Site-directed mutagenesis/Single site. From OpenWetWare. Revision as of 10:59, 24 November 2008 by Tk. (talk , contribs) ... Back to Site-directed mutagenesis Note that this is a work in progress and needs to be verified against my lab notebooks. -- ... This procedure is primarily derived from the Stratagene QuikChange Site-Directed Mutagenesis manual with some modifications ... Retrieved from "https://openwetware.org/mediawiki/index.php?title=Knight:Site-directed_mutagenesis/Single_site&oldid=264831" ...
  https://openwetware.org/wiki/?title=Knight:Site-directed_mutagenesis/Single_site&oldid=264831
*  'Round-the-horn site-directed mutagenesis -...
... this is the case for any site-directed protocol). Finally, as opposed to standard site-directed mutagenesis, this procedure ... Round-the-horn site-directed mutagenesis. From OpenWetWare. Revision as of 04:55, 31 August 2012 by Smoore. (talk , contribs) ( ... Retrieved from "https://openwetware.org/mediawiki/index.php?title=%27Round-the-horn_site-directed_mutagenesis&oldid=621679" ... Round-the-horn is a PCR-based mutagenesis. The muations are contained in one or both of the primers. The primers are ...
  https://openwetware.org/wiki/?title=
*  Knight:Site-directed mutagenesis/Multi site - OpenWetWare
Knight:Site-directed mutagenesis/Multi site. From OpenWetWare. Revision as of 12:14, 23 August 2007 by Reshma P. Shetty. (talk ... This procedure is primarily derived from the Stratagene QuikChange Multi Site-Directed Mutagenesis manual with some ... Retrieved from "https://openwetware.org/mediawiki/index.php?title=Knight:Site-directed_mutagenesis/Multi_site&oldid=144493" ... This protocol permits mutation at multiple sites simultaneously with only a single oligo per site. ...
  https://openwetware.org/wiki/?title=Knight:Site-directed_mutagenesis/Multi_site&oldid=144493
*  Site-Directed Mutagenesis - Cloning and Mutagenesis - GENEWIZ
SITE-DIRECTED MUTAGENESIS. GENEWIZ can increase your research productivity by performing your time-consuming site-directed ... Our customized mutagenesis services provide a fail-safe approach to obtain mutant constructs quickly, with 100% accuracy, thus ...
  https://www.genewiz.com/en/Public/Services/Cloning-Mutagenesis/Site-Directed-Mutagenesis
*  Site Directed In Vivo Mutagenesis - Princeton University Library Catalog
This thesis can be viewed in person at the Mudd Manuscript Library. To order a copy complete the Senior Thesis Request Form. For more information contact mudd@princeton.edu ...
  https://catalog.princeton.edu/catalog/dsp01z603r0481
*  Expression and site-directed mutagenesis of hepatic glucokinase | Biochemical Journal
Expression and site-directed mutagenesis of hepatic glucokinase. A J Lange, L Z Xu, F Van Poelwijk, K Lin, D K Granner, S J ... Expression and site-directed mutagenesis of hepatic glucokinase. A J Lange, L Z Xu, F Van Poelwijk, K Lin, D K Granner, S J ... Expression and site-directed mutagenesis of hepatic glucokinase Message Subject (Your Name) has forwarded a page to you from ... Your Name) thought you would like to see this page from the Biochemical Journal web site. ...
  http://www.biochemj.org/content/277/1/159
*  Site-directed mutagenesis of a lytic polysaccharide monooxygenase from Micromonospora aurantiaca
... Gullesen, Jonathan ... positions in the sequences of specific C1 oxidizing cellulose-active AA10 proteins were targeted for site-directed mutagenesis ...
  https://brage.bibsys.no/xmlui/handle/11250/2398688
*  Delineation of Subunit and Receptor Contact Sites by Site-Directed Mutagenesis of hCGβ | SpringerLink
Puett D., Huang J., Xia H. (1994) Delineation of Subunit and Receptor Contact Sites by Site-Directed Mutagenesis of hCGβ. In: ... Delineation of Subunit and Receptor Contact Sites by Site-Directed Mutagenesis of hCGβ. ... Site-directed mutagensis of virtually any plasmid by eliminating a unique site. Anal Biochem 1992; 200: 81-8.PubMedCrossRef ... El-Deiry S, Kaetzel D, Kennedy G, Nilson J, Puett D. Site-directed muta-genesis of the human chorionic gonadotropin β-subunit: ...
  https://link.springer.com/chapter/10.1007/978-1-4613-8386-4_12
*  Difference between revisions of "Lidstrom:Site-Directed Mutagenesis" - OpenWetWare
Retrieved from "https://openwetware.org/mediawiki/index.php?title=Lidstrom:Site-Directed_Mutagenesis&oldid=588128" ... Kunkel Mutagenesis. *Justin Siegel at the Baker lab is our local expert. The UW iGem team uses this method. ...
  https://openwetware.org/wiki/?title=Lidstrom:Site-Directed_Mutagenesis&diff=588128&oldid=588127
*  Problems with Quick change lightning multi site directed mutagenesis kit - Molecular Cloning - BioForum
I am using quick change lightning multi site directed mutagenesis kit from stratagene, i want to mutate 5nt , my plasmid+insert ... Problems with Quick change lightning multi site directed mutagenesis kit - posted in Molecular Cloning: ... Problems with Quick change lightning multi site directed mutagenesis kit. Started by Adnan, Sep 18 2009 07:13 AM ... I am using quick change multi site directed mutagenesis kit.........and am following its protocol only, nt a mixed protocol.. I ...
  http://www.protocol-online.org/forums/topic/10371-problems-with-quick-change-lightning-multi-site-directed-mutagenesis-kit/
*  Difference between revisions of "Richard Lab:Site Directed Mutagenesis" - OpenWetWare
An efficient one-step site-directed and site-saturation mutagenesis protocol. Nucleic Acids Res. 2004; 32(14): e115. ... This protocol only uses one oligo per mutation site (cuts the oligo cost in half) but can mutate more than one site at a time. ... This protocol only uses one oligo per mutation site (cuts the oligo cost in half) and can mutate more than one site at a time. ... Retrieved from "https://openwetware.org/mediawiki/index.php?title=Richard_Lab:Site_Directed_Mutagenesis&oldid=518978" ...
  https://openwetware.org/wiki/?title=Richard_Lab:Site_Directed_Mutagenesis&diff=518978&oldid=518976
*  IJMS | Free Full-Text | Development of Therapeutic Chimeric Uricase by Exon Replacement/Restoration and Site-Directed...
... site-directed mutagenesis uricase; exon replacement/restoration; site-directed mutagenesis ... After site-directed mutagenesis, H1-2P3H4P5-6H7-8 (E24D & E83G) with increased homology (91.45%) with dHU and higher activity ... Development of Therapeutic Chimeric Uricase by Exon Replacement/Restoration and Site-Directed Mutagenesis. Guangrong Xie †. ... In order to develop a more "human-like" uricase for therapeutic use, exon replacement/restoration and site-directed mutagenesis ...
  http://www.mdpi.com/1422-0067/17/5/764