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*  A single domain antibody fragment that recognizes the adaptor ASC defines the role of ASC domains in inflammasome assembly | JEM
VHHs can induce or selectively bind distinct conformations, mask epitopes required for protein-protein interactions, or inhibit ... VHHs are stable in the absence of disulfide bonds or glycosylation and can thus be easily produced in bacteria or expressed in ... Fluorescently labeled monomeric MBP-caspase-1CARD-SUMO (4 µM) was mixed with oligomeric GFP-ASCCARD (1.1 µM) preincubated with ... A single domain antibody fragment that recognizes the adaptor ASC defines the role of ASC domains in inflammasome assembly. ...
  http://jem.rupress.org/content/early/2016/04/05/jem.20151790
*  Single-domain antibody - Wikipedia
... is an antibody fragment consisting of a single monomeric variable antibody domain. Like a whole antibody, it is able to bind ... Although most research into single-domain antibodies is currently based on heavy chain variable domains, Nanobodies derived ... are also possible targets for orally available single-domain antibodies. Detergent-stable species targeting a surface protein ... "Escherichia coli F4 fimbriae specific llama single-domain antibody fragments effectively inhibit bacterial adhesion in vitro ...
  https://en.wikipedia.org/wiki/Single-domain_antibody
*  Chimeric camel/human heavy-chain antibodies protect against MERS-CoV infection | Science Advances
Nanobodies: Natural single-domain antibodies. Annu. Rev. Biochem. 82, 775-797 (2013).. ... MERS-CoV polyclonal antibodies, an irrelevant VHH, and four VHHs were then tested for their ability to bind to these S1 ... 3 MERS-CoV-neutralizing efficacy of monomeric VHHs and chimeric antibodies on Huh-7 cells.. MERS-CoV (EMC isolate) was ... This fragment is about 14 kDa in size, is relatively stable, and can be produced with high yields in prokaryotic systems (18, ...
  http://advances.sciencemag.org/content/4/8/eaas9667.full
*  Frontiers | Intracellular Crosslinking of Filoviral Nucleoproteins with Xintrabodies Restricts Viral Packaging | Immunology
... while monomeric sdAb showed ambivalent partition. Infection of stable Vero cell lines expressing dimeric sdAb specific for ... likely benefit from transitioning away from intracellular expression of crosslinking sdAb to exogenous delivery of antibody. By ... with purified sdAb constructs showed NP aggregation occurred in a genus specific manner with dimeric and not monomeric sdAb. A ... with purified sdAb constructs showed NP aggregation occurred in a genus specific manner with dimeric and not monomeric sdAb. A ...
  https://www.frontiersin.org/articles/10.3389/fimmu.2017.01197/full
*  Frontiers | Fc Engineering for Developing Therapeutic Bispecific Antibodies and Novel Scaffolds | Immunology
Promisingly, Fc antigen binding fragment [Fcab] and monomeric antibody or half antibody may be particularly advantageous to ... Promisingly, Fc antigen binding fragment [Fcab] and monomeric antibody or half antibody may be particularly advantageous to ... to antibody fragments. Impressively, antibody fragments such as bispecific T-cell engager [BiTE], bispecific killer cell ... to antibody fragments. Impressively, antibody fragments such as bispecific T-cell engager [BiTE], bispecific killer cell ...
  https://www.frontiersin.org/articles/10.3389/fimmu.2017.00038/full
*  Frontiers | Extensive Basal Level Activation of Complement Mannose-Binding Lectin-Associated Serine Protease-3: Kinetic...
All MASPs have the same six-domain structure (4, 7). The C-terminal SP domain is preceded by five regulatory domains in the ... or a MASP-3-specific antibody. Both antibodies were developed against the serine protease (SP) domain of the corresponding ... Factor D (FD), a single-domain SP, is a key enzyme for the alternative pathway. FD has only one natural substrate, factor B (FB ... MASPs are expected to bind preferably to MBL-Sepharose as a result of the 3 to 1, immobilized rMBL to soluble PRM ratio. After ...
  https://www.frontiersin.org/articles/10.3389/fimmu.2017.01821/full
*  Application # 2016/0194410. BINDING AGENT - Patents.com
... domains, single domain antibodies (see: Hey, T. and Fiedler, E., et al., Trends Biotechnol. 23 (2005) 514-522), and monovalent ... 0004] Monoclonal antibodies (mAbs), being directed towards single epitopes on the antigen, usually bind with affinities which ... capable of forming a stable duplex via multiple base pairing. Stable means that the melting temperature of the duplex is higher ... In such spacer monomeric units within a spacer are linked in each case via a phosphate or phosphate analog moiety. [0078] The ...
  http://patents.com/us-20160194410.html
*  WO2014004427A2 - Anti-egfr antibodies and uses thereof - Google Patents
... the antibodies are fully human antibodies that bind to human EGFR with high affinity. In certain embodiments, the antibodies of ... The antibodies of the invention are useful for the treatment of various cancers as well as other EGFR-related disorders. ... present invention are capable of inhibiting the growth of tumor cells expressing high levels of EGFR and/or inducing antibody- ... The present invention provides antibodies that bind to EGFR and methods of using same. According to certain embodiments of the ...
  https://patents.google.com/patent/WO2014004427A2/en
*  Discovery Biology News
In a report by Tereshko et al., camelid single domain antibodies, specifically the variable heavy chain (VHH) domains can be ... Discovering antibody therapeutics and new modalities requires high quality stable cell lines for antibody lead identification ... A study published in FASEB demonstrated that monomeric variable heavy-chain domain of camel homodimeric antibodies (mVHH), can ... Targeting fibroblast activation protein in tumor stroma with chimeric antigen receptor T cells can inhibit tumor growth and ...
  https://www.genscript.com/DiscoveryBiology_news.html
*  William Weis | Stanford Medicine Profiles
Here we report stabilization of two distinct β2AR conformations using single domain camelid antibodies (nanobodies)-a ... demonstrating that a single Dkk1 molecule can bind to both portions of the LRP6 ectodomain and thereby inhibit different Wnts. ... The 1056-amino-acid N-terminal region of DP contains a plakin domain common to members of the plakin family. Plakin domains ... stable F-actin-binding state. The data also imply that αE-catenin must be activated in order to bind to vinculin. ...
  https://med.stanford.edu/profiles/william-weis
*  SMART: Secondary literature for RICIN domain
... can be dissected into amino-terminal and carboxyl-terminal domains to form single binding domains (SBDs) of B chain, each of ... The products were shown to be N-glycosylated and produced in a soluble stable form. Also, they retained the ability to bind ... All the mutants showed immunoreactivity with a panel of anti-RTB monoclonal and polyclonal antibodies. Single-lectin site ... These include the monomeric enzymes PAP, PAP-S, PAP-II, and dodecandrin and the enzymatic A chains from the heterodimeric ...
  http://smart.embl-heidelberg.de/smart/show_secondary.cgi?domain=RICIN
*  IJMS | Free Full-Text | Bispecific Antibodies as a Development Platform for New Concepts and Treatment Strategies | HTML
Bispecific antibodies that can simultaneously bind to cell surface antigens and payloads are a very ideal delivery system for ... The single-chain-based bispecific antibodies usually bridge tumor cells with immune cells and form an immunological synapse ... Bispecific antibodies that can inhibit two correlated signaling molecules at the same time can be developed to overcome ... Bispecific antibodies in the IgG format include asymmetric bispecific antibodies and homodimerized bispecific antibodies, all ...
  http://www.mdpi.com/1422-0067/18/1/48/htm
*  ABLYNX NV - Patent applications
The binding domains or binding units may in particular be a domain antibody, a single domain antibody, a dAb or a Nanobody®. ... STABLE FORMULATIONS OF IMMUNOGLOBULIN SINGLE VARIABLE DOMAINS AND USES THEREOF - The present invention relates to stable ... AMINO ACID SEQUENCES THAT BIND TO A DESIRED MOLECULE IN A CONDITIONAL MANNER - The present invention relates to amino acid ... In particular, the biological materials of the present invention inhibit the biological activity of the P2X7 receptor, such as ...
  http://www.patentsencyclopedia.com/assignee/ablynx-nv/
*  Anti-ErbB3 Antibodies and Uses Thereof - Patent application
... domain-specific antibodies, single domain antibodies, domain-deleted antibodies, chimeric antibodies, CDR-grafted antibodies, ... Next, the ability of a test antibody to bind to the ErbB3 molecule is assessed. If the test antibody is able to bind to ErbB3 ... antibody may contain a monomeric VH or VL domain. [0038] In certain embodiments, an antigen-binding fragment of an antibody may ... antibodies possessing human variable domains and mouse constant domains) were obtained; exemplary antibodies generated in this ...
  http://www.patentsencyclopedia.com/app/20160144029
*  Antibodies That Bind OV064 and Methods of Use Therefor - Patent application
... which consists of a VH domain; (vii) a single domain functional heavy chain antibody, which consists of a VHH domain (known as ... The anti-OV064 antibody can inhibit, e.g., competitively inhibit, the binding of an antibody described herein, e.g., sc77, ... consisting of the VH and CH1 domains; (iv) a Fv fragment consisting of the VL and VH domains of a single arm of an antibody, (v ... The DTPA-conjugated antibodies are stable at least 10 weeks at -80° C. and are stable at least 120 hrs after labeling. Both ...
  http://www.patentsencyclopedia.com/app/20090208489
*  Methods and Compositions for Generating Bioactive Assemblies of Increased Complexity and Uses - Patent application
... and includes antibody fragments such as Fab', Fab, F(ab')2, single domain antibodies (DABs), Fv, scFv (single chain Fv), and ... Because the R subunits have been isolated only as stable dimers and AKAPs bind only to dimeric R subunits, a unique feature of ... The recombinant host cells synthesize a single polypeptide chain with a linker peptide bridging the two V domains. Methods for ... monomeric subunit linked to DDD2 and the third one consisting of a monomeric subunit linked to AD3 (designated as Y), resulting ...
  http://www.patentsencyclopedia.com/app/20090202433
*  IJMS | Free Full-Text | Novel Strategies for Drug Discovery Based on Intrinsically Disordered Proteins (IDPs) | HTML
Structure and Properties of a Complex of alpha-Synuclein and a Single-Domain Camelid Antibody. J. Mol. Biol 2010, 402, 326-343 ... There are three structural domains in p53 [89]: N-terminal translational activation domain, central DNA binding domain, and C- ... Human α-synuclein is a 140 amino acid-protein and its normal function is to bind to the surface of synaptic vesicles [31,32]. ... Drugs, such as FTID, decreases tau phosphorylation and inhibit neurofibrillary degeneration.. P53 protein [64]: p53 is at the ...
  http://www.mdpi.com/1422-0067/12/5/3205/htm
*  DiVA - Search result
Both the N-terminal and C-terminal domains of calmodulin can independently bind to and inhibit the DNA binding of bHLH proteins ... Previous experiments with two single-domain proteins showed that macromolecular crowding can stabilize dramatically toward heat ... IdeS was stable above pH 10 but not at acidic pH. It exhibited an activity maximum around 37 degrees C and a decreased thermal ... Experiments on monomeric proteins have shown that macromolecular crowding can stabilize toward heat perturbation and also ...
  http://umu.diva-portal.org/smash/resultList.jsf?af=%5B%5D&aq=%5B%5B%7B%22journalId%22%3A%221356%22%7D%5D%5D&aqe=%5B%5D&aq2=%5B%5B%5D%5D&language=en&query=
*  RECOMBINANT RENEWABLE POLYCLONAL ANTIBODIES - Patent application
Each light chain contains a single constant domain (CL), while each heavy chain contains three constant domains, CH1, CH2 and ... It has been found that antibodies often bind targets that are much larger than the monomeric form of the intended target, in ... and libraries based on stable scaffolds tend to produce antibodies that are more stable and well expressed (Fellouse et al., J ... The ability to completely inhibit the Gall promoter and hence antibody expression during yeast display and secretion, allows ...
  http://www.patentsencyclopedia.com/app/20130085072
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
However, as another opportunity, these interesting and stable single-domain antibodies8 can be easily engineered with different ... Both the usefulness and limitation of the method depends on whether the antibodies employed are able to bind to native epitopes ... The hydrophobic transmembrane domains make the proteins insoluble in aqueous buffer. Before a transmembrane protein can be ... These include the design of monomeric proteins for increased stability and complexes for increased binding affinity. To ...
  https://www.jove.com/visualize/abstract/25811612/inhibition-of-type-vi-secretion-by-an-anti-tssm-llama-nanobody
*  Michael Levitt's Profile | Stanford Profiles
The anti-Tac monoclonal antibody is known to bind to the p55 chain of the human interleukin 2 receptor and to inhibit ... The number of sequences matched by a single UFW (sequences with single domain architectures) increased as slowly as the number ... It is partially derived from the SCOP database of protein domains, and it includes sequences for each domain as well as other ... whereas the other two mutants were either less stable or equally stable. Measurement of the equilibrium constants for the ...
  https://profiles.stanford.edu/michael-levitt
*  SMART: Secondary literature for CA domain
Anti-E-cadherin monoclonal antibody can bind to E-cadherin (uvomorulin) and inhibit cell-cell adhesion through the inhibition ... The cadherins typically consist of five tandemly repeated extracellular domains, a single membrane-spanning segment and a ... The monomeric state of this isolated domain is demonstrated by light scattering and sedimentation analysis. Seven beta-strands ... By stable transfection into E-cadherin-deficient cell lines, we show that cadherins are both necessary and sufficient for ...
  http://smart.embl.de/smart/show_secondary.cgi?domain=CA
*  COMPOSITIONS AND METHODS OF USE FOR THERAPEUTIC ANTIBODIES - Patent application
... consisting of the VH and CH1 domains; a Fv fragment consisting of the VL and VH domains of a single arm of an antibody; a dAb ... In certain embodiments, the antibodies bind the target protein IL12Rβ1 and inhibit IL12 dependent INF-γ production in human ... the human antibodies of the invention into nanobody or single domain antibody framework sequences, as described for example in ... The stable subclones can then be cultured in vitro to generate small amounts of antibody in tissue culture medium for ...
  http://www.patentsencyclopedia.com/app/20120034231
*  Neurodegenerative Diseases | Leaders in Pharmaceutical Business Intelligence (LPBI) Group
Choosing the right antibody platform can speed the discovery of a new therapeutics against these new targets. Single domain ... Although it had well-characterized protein domains such as coiled-coil domains and leucine-zipper domains, its function was ... A study published in FASEB demonstrated that monomeric variable heavy-chain domain of camel homodimeric antibodies (mVHH), can ... Lu et al. show that K36M mutant histones are oncogenic, and they inhibit the normal methylation of this same residue in wild- ...
  https://pharmaceuticalintelligence.com/category/neurodegenerative-diseases/
*  Fibronectin Type III Domain Based Scaffold Compositions, Methods And Uses - Patent application
... single domain antibodies, and fragments thereof. Functional fragments include antigen-binding fragments that bind to a ... sequence alignment of only the most stable FN3 domains was produced in order to maximize the potential of producing a stable ... in agreement with the predicted masses for the monomeric proteins. [0186]The folding and thermal stability of each FN3 domain ... inhibit enzymatic degradation. Formulations for delivery of hydrophilic agents including proteins and protein scaffolds and a ...
  http://www.patentsencyclopedia.com/app/20100255056