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*  Open access articles from Soft Matter Home
Using Brownian motion to measure shape asymmetry in mesoscopic matter using optical tweezers Basudev Roy, Argha Mondal, Sudipta ... The bending modulus κ is known to be a crucial parameter for the stability of the droplet phase. By combination of droplet ... Molecular ordering in the high-temperature nematic phase of an all-aromatic liquid crystal Francesco Vita, Maruti Hegde, ... Mechanical spectroscopy of retina explants at the protein level employing nanostructured scaffolds S. Mayazur Rahman, Andreas ...
  http://pubs.rsc.org/en/journals/articlecollectionlanding?sercode=sm&themeid=e297b895-0853-410c-8079-aa5917ba7475
*  Live cell imaging of mechanotransduction | Journal of The Royal Society Interface
... it is now convenient to visualize molecular signals at subcellular levels in live cells. Traditional assays to detect/measure ... Relatively local stretch can be conveniently applied on cells with laser/optical tweezers or magnetic tweezers. Optical ... When the tip of the force sensor is inserted into the droplet of FN solution to suck the liquid and result in stretching the FN ... The mechanics of cell-material interactions was evaluated by the FRET technique at the molecular level. The proliferation and ...
  http://rsif.royalsocietypublishing.org/content/early/2010/03/29/rsif.2010.0042.focus
*  Blog on Optical Tweezers: February 2010
Molecular Biology of the Cell (9) Molecular Cell (4) Molecular Crystals and Liquid Crystals (6) Molecular Microbiology (2) ... We used optical tweezers to measure the rupture force between HU proteins and Lon; HUα, HUβ, and HUβ-A20D can bind to Lon, and ... Side imaging was used in conjunction with imaging in the plane of the optical trap. Droplet sizing was performed using cavity- ... and discusses how optical tweezers can be employed to quantify cell-nanoparticle interactions at the single particle level. ...
  http://opticaltweezers.blogspot.com/2010/02/
*  Analyzing Large Protein Complexes by Structural Mass Spectrometry | Protocol
Place the capillary under an optical microscope and trim the tip, using the sharp AA-type tweezers. ... The droplet size is a function of the tip diameter, which should be ~1 μm. Small droplets lead to more efficient ionization, ... It is recommended that you set the m/z range to the maximum level, and then reduce it to the desired values. We further suggest ... Alberts, B. The cell as a collection of protein machines: preparing the next generation of molecular biologists. Cell. 92, (3 ...
  https://www.jove.com/video/1954/analyzing-large-protein-complexes-by-structural-mass-spectrometry
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... optical tweezers, flow stretching, and magnetic tweezers. Amongst these approaches, magnetic tweezers have distinguished ... a direct quantitative measure for the protein activity and abundance of substrates or binding sites at the single-cell level. ... Molecular Biology, Issue 91, plusTipTracker, microtubule plus-end-tracking proteins, EB1, growth cone, Xenopus laevis, live ... In this assay, neutrophils migrate directly from the blood droplet, through small channels, towards the source of ...
  https://www.jove.com/visualize/abstract/19956649/systematic-differences-in-impact-across-publication-tracks-at-pnas
*  Download Alien Encounters
Prerequisite of practice; transition tweezers findings in new military reflection projects. download of application; optical ... A molecular download alien of re-engineer in Africa is that Leptospira is There is official upset understanding in index ... now 13 queue in link( more on the droplet). lost from and been by Amazon. Please call a sexual UK gene. Please become a ... This got us with a effective' summer' of full products that can agree methods of select Household to level. We speak included ...
  http://pd19.org/ebook/download-alien-encounters
*  New England Complex Fluids Workgroup
... which is a direct measure of the viscoelastic response of the material.. Keywords: Microrheology, Biopolymer, Optical tweezers ... The goal of this research is to establish molecular-level design rules as to how chemical sequence can modulate the formation ... We have developed a droplet-based microfluidics technology that is capable of first encapsulating single cells in droplet, ... In this study, we use optical tweezers to oscillate a particle inside reconstituted biopolymer networks and by measuring the ...
  http://www.complexfluids.org/necf/abstracts.php?meetingID=75
*  Blog on Optical Tweezers: January 2010
Mechanical forces play important roles in the regulation of various biological processes at the molecular and cellular level, ... If the object is relatively easy to deform, as is, e.g., a low surface tension droplet, the resulting deformation is easily ... combination of opposing optical and fluid drag forces on colloidal samples within microfluidic environments to both measure ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (4) Optical Memory and Neural Networks (1) Optical ...
  http://opticaltweezers.blogspot.com/2010/01/
*  Microfluidic tools for cell biological research
... optical tweezers [116], or complex dedicated force machines [117]. Most of these apparatus measure global and local forces of ... This effect smooths the molecular distribution and create molecular gradient inside the cell instead of a straight molecular ... Picture of a cell trapping array using hydrodynamic trap and zoom on a single cell trap [168]. (B) Droplet based yeast culture ... Microfluidic technology enables studies of cell behavior from single- to multi-cellular organism level with precise and ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2998071/
*  Publications | Stavans Lab
By scattering a focused laser beam from a particle observed in an optical microscope, we measure its fluctuations via the ... Phase diagrams of droplet volume fraction versus polymer concentration are presented. At large droplet volume fractions where ... Joel Stavans (2008). The Accuracy of Molecular Processes The Case of Homologous Recombination. Evolution From Cellular To ... The timing of events in cells committed to lysis is independent of the level of activation of the SOS response, suggesting that ...
  http://www.weizmann.ac.il/complex/stavans/publications
*  Patente US20110229872 - Microfabricated Cell Sorter - Google Patentes
... can be sorted into an appropriate branch channel based on the presence or amount of a detectable signal such as an optical ... reporter-labeled cells can be sorted by the presence or level of reporter on the cells. The device includes a chip having a ... Optical Trapping. * [0175] In another embodiment, an optical trap, or laser tweezers, may be used to sort or direct cells in a ... In the embodiment of this example, the level of reporter signal is measured at the detection region using an optical detector, ...
  http://www.google.es/patents/US20110229872
*  Blog on Optical Tweezers: November 2009
Nano-mechanical measurements and manipulations at the single-cell and single-molecular levels using the atomic force microscope ... The optical properties of the droplet are determined using stimulated Raman scattering. The droplet is in thermodynamic ... and optical tweezers (OT) are combined to measure the interaction forces between blank SiO2 surfaces in aqueous ionic solutions ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (3) Optical Memory and Neural Networks (1) Optical ...
  http://opticaltweezers.blogspot.com/2009_11_01_archive.html
*  Blog on Optical Tweezers: November 2011
Molecular Biology of the Cell (8) Molecular Cell (4) Molecular Crystals and Liquid Crystals (6) Molecular Microbiology (2) ... by manipulating via optical tweezers at the single-cell level. Theoretical approaches were developed to model the dynamic and ... A defocused-edge detection method was devised to measure the orientation of the rotated cell from the corresponding phase- ... Mechanical stochastic tug-of-war models cannot explain bidirectional lipid-droplet transport ...
  http://opticaltweezers.blogspot.com/2011_11_01_archive.html
*  Books by Subject - Lane Medical Library - Stanford University School of Medicine
At the molecular level p53 plays a central role as a transcriptional activator, and p53 has two amino-terminal transactivation ... An Improved Optical Tweezers Assay for Measuring the Force Generation of Single Kinesin Molecules -- Seeded Microtubule Growth ... How to measure microtubule dynamics? / Anne Straube -- Purification of tubulin from porcine brain / Christopher Gell [and ... Microscopic analysis of lipid droplet metabolism and dynamics in yeast / Heimo Wolinski, Sepp D. Kohlwein -- Ch. 12. Use of ...
  http://lane.stanford.edu/biomed-resources/ebsubjectbrowse.html?m=Cell%20Biology&page=3
*  Blog on Optical Tweezers: October 2009
However, the detailed mechanism, at the molecular level, leading to UFP cytotoxicity is not yet fully understood. To address ... We applied oscillatory optical tweezers to measure the differential stiffness of dsDNA molecules, interacting with RecA, as a ... 2) Positive/negative photophoresis on a droplet: We show that a droplet is driven by a laser beam, either toward and backward ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (3) Optical Memory and Neural Networks (1) Optical ...
  http://opticaltweezers.blogspot.com/2009_10_01_archive.html
*  DEVICE AND METHOD FOR PRESSURE-DRIVEN PLUG TRANSPORT - Patent application
Optical detection of molecular characteristics or the tag associated with a characteristic or property that is chosen for ... optical tweezers, and combinations thereof, without regard for any particular theory or mechanism of action. [0089] The term " ... an optical microscope. Long exposures (i.e., about 2 seconds) have been used to measure fast (i.e., about 2 milliseconds) ... Vapor Diffusion Protein Crystallization in Capillaries by an Alternating Droplet System [0429] The principle of transferring ...
  http://www.patentsencyclopedia.com/app/20110177586
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Nanomanipulation of Single RNA Molecules by Optical Tweezers. Authors: William Stephenson, Gorby Wan, Scott A. Tenenbaum, Pan T ... More and more, molecular biologists require consistent small-volume analysis of nucleic acid samples for their experiments. The ... Advantages of the ultra-thin layer method over other sample deposition approaches (e.g. dried droplet) are that it provides (i ... Shape changes in the discriminant function analysis are based on a statistical measure, and therefore can be evaluated by a p- ...
  https://www.jove.com/visualize/abstract/23516637/analysis-laboratory-repeat-critical-values-at-large-tertiary-teaching
*  Blog on Optical Tweezers: January 2013
Molecular Biology of the Cell (9) Molecular Cell (4) Molecular Crystals and Liquid Crystals (6) Molecular Microbiology (2) ... In recent years, optical tweezers have become one of the tools to measure the mechanical properties of living cells. In this ... Inherent Brownian motion limits current force spectroscopy methods from observing possible bond level structural changes. We ... the ability of the droplet to uptake water from the air depends on the solute weight percent of sodium chloride present in a ...
  http://opticaltweezers.blogspot.com/2013/01/
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... optical tweezers, flow stretching, and magnetic tweezers. Amongst these approaches, magnetic tweezers have distinguished ... magnetic tweezers and the freely-orbiting magnetic tweezers, which makes it possible to directly measure the torque stored in a ... directed molecular evolution will allow the creation of custom enzymes that will serve as tools of "molecular surgery" and ... It is also observed that when nozzles are aimed at the inlet at shallow angles, the droplet momentum can cause additional ...
  https://www.jove.com/visualize/abstract/21559524/eugene-domain-specific-language-for-specifying-constraining-synthetic
*  Blog on Optical Tweezers: September 2009
Experiments at the single molecular level have shown that unfolding forces for parallel and antiparallel structures (Funfold: ... We investigate the mechanics and the dynamics of RBCs by a unique noninvasive technique, using weak optical tweezers to measure ... It also facilitates the use of an isolated droplet of known composition as a sensitive probe of the gas phase conditions in an ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (3) Optical Memory and Neural Networks (1) Optical ...
  http://opticaltweezers.blogspot.com/2009_09_01_archive.html
*  Blog on Optical Tweezers: January 2011
... molecular interactions of actin with myosin but are an artifact of the Brownian motion associated with these molecular level ... We used optical tweezers to measure the strength and attachment lifetime of single myo1c molecules as they bind beads coated ... a nematic droplet held in laser tweezers. The identification and spatial evolution of such a topological fingerprint is ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (3) Optical Memory and Neural Networks (1) Optical ...
  http://opticaltweezers.blogspot.com/2011_01_01_archive.html
*  ACPD - Recent
The role of droplet sedimentation in the evolution of low level clouds over Southern West Africa Christopher Dearden, Adrian ... index of insoluble organic extracts from atmospheric aerosol over the visible wavelength range using optical tweezers Rosalie H ... Molecular distribution and stable carbon isotopic compositions of dicarboxylic acids and related SOA from biogenic sources in ... We collected a 15 m ice core from the Canadian High Arctic to measure a 38-year deposition record of PFAAs, proving information ...
  https://www.atmos-chem-phys-discuss.net/
*  Blog on Optical Tweezers: May 2012
We measure the fluctuations of the position of a Brownian particle confined by an optical trap in an aging gelatin droplet ... Molecular Biology of the Cell (9) Molecular Cell (4) Molecular Crystals and Liquid Crystals (6) Molecular Microbiology (2) ... Thus far, for intact cells sustained oscillations have only been observed at the population level, i.e. for synchronized ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (4) Optical Memory and Neural Networks (1) Optical ...
  http://opticaltweezers.blogspot.com/2012/05/
*  Blog on Optical Tweezers: September 2010
Molecular Biology of the Cell (8) Molecular Cell (4) Molecular Crystals and Liquid Crystals (6) Molecular Microbiology (2) ... Droplet manipulation and trapping potential in such a two-dimensional anisotropic system show that optical trapping has two ... By combining experimental data with a physical model and an optimization algorithm for analysis, we measure the 10 unique ... The cells exhibit high levels of viability, proliferation, three- dimensional (3D) cell morphology and other functions in the ...
  http://opticaltweezers.blogspot.com/2010_09_01_archive.html
*  Blog on Optical Tweezers: April 2012
... steps of the simulation gives a three-dimensional visual insight into these types of experiment at the molecular level. DOI ... We present a simple method to calibrate optical tweezers by using only videomicroscopy to measure the position autocorrelation ... Smaller droplet sizes were preferentially selected with a 1.7 μm core diameter compared to cores of 2.7 μm and 4.5 μm. Further ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (3) Optical Memory and Neural Networks (1) Optical ...
  http://opticaltweezers.blogspot.com/2012_04_01_archive.html