*  Isolation of Sf21/Sf9 microsomes (without clumping)
... Mike Autry jma at ddt.biochem.umn.edu Tue Nov 29 16:52:41 EST 2005 * ... The insect cell microsomes are clumpy when isolated either in solutions containing high salt (0.6 M KCl, 250 mM sucrose, 3 mM ... Why do the microsomes clump so much? Are there solutes to add (pyrophosphate, NaI, etc) that would allow easy and complete ... Final pellets (Sf21 microsomes) were resuspended at approximately 5 mg/ml in 250 mM sucrose, 30 mM histidine (pH 7.4) and ...
  http://www.bio.net/bionet/mm/methods/2005-November/099937.html
*  Microsome
Male minipig intestine microsomes. Cell Fractions (Vial containing 5 mg of proteins). MIC356. Biopredic. Enquire. order. ... Human jejunum microsomes. Cell Fractions (Vial containing 5 mg of proteins). MIC316. Biopredic. Enquire. order. ... Cytochrome P450(CYP2C9) Microsome. Reagent (5 nmol). PV-P2378. MBL. £230.00. order. ... Cytochrome P450(CYP3A4) Microsome. Reagent (5 nmol). PV-P2377. MBL. £230.00. order. ...
  https://www.caltagmedsystems.co.uk/pricing_ordering/index.php?PT_ID=3&PG_ID=18
*  Metabolism of Cyclophosphamide by Rat Hepatic Microsomes | Cancer Research
Microsomes from livers of female rats and mice metabolized cyclophosphamide with a Vmax of 0.66 and 9.69 µmoles/g/hr, ... Metabolism of Cyclophosphamide by Rat Hepatic Microsomes Message Subject (Your Name) has forwarded a page to you from Cancer ... The conversion of cyclophosphamide to its alkylating metabolite(s) by microsomes obtained from male rat liver was characterized ... to be greatly depressed if previously frozen rather than fresh microsomes were used. Cyclophosphamide bound to microsomal ...
  http://cancerres.aacrjournals.org/content/31/6/901
*  Microsomes and Drug Oxidations, Vol. 6 | Rent 9780850662825 | 0850662826
Boobis, A. R. is the author of 'Microsomes and Drug Oxidations, Vol. 6', published 1985 under ISBN 9780850662825 and ISBN ...
  https://www.valorebooks.com/textbooks/microsomes-and-drug-oxidations-vol-6/9780850662825
*  Liver Microsomes, S9 Fractions and Cytosol | Thermo Fisher Scientific - JP
... microsomes & other liver subcellular fractionsLarge pooled lots for reproducible, long-term studiesFractions carefully isolated ... Table 3 - Kinetic parameters for the GIBCO® 50 donor human liver microsome pool Isoform Metabolite Km(μM) Vmax (nmol/min/mg) ... Human microsome pools and subcellular fractions Subcellular fractions, derived from the endoplasmic reticulum of liver, contain ... Table 2 - Cytochrome P450 and Phase II enzymes tested in GIBCO® human microsome pools Enzyme Monitored Marker Substrate ...
  https://www.thermofisher.com/jp/en/home/industrial/pharma-biopharma/drug-discovery-development/microsomes-s9-fractions-cytosol.html
*  Cytochrome P-420: Tubular Aggregates from Hepatic Microsomes | Science
Cytochrome P-420: Tubular Aggregates from Hepatic Microsomes Message Subject. (Your Name) has forwarded a page to you from ... Aggregates were formed when clear supernatants from hepatic microsomes that had been treated with steapsin were desalted and ... drugs to give the characteristic difference spectra normally seen only with cytochrome P-450 contained in intact microsomes. ...
  http://science.sciencemag.org/content/165/3900/1371
*  Overexpression of Catalase Enhances Benzo(a)pyrene Detoxification in Endothelial Microsomes. | Sigma-Aldrich
Overexpression of catalase did not alter the distribution of each of these enzymes in the microsomes. In contrast to our ... Overexpression of Catalase Enhances Benzo(a)pyrene Detoxification in Endothelial Microsomes.. [Fang Yang, Hong Yang, Aramandla ... Our data showed that endothelial microsomes were enriched in cytochrome P450 (CYP) 1A1, CYP1B1 and epoxide hydrolase 1 (EH1), ... greater than that of the BaP diols/diones after exposure of microsomes to BaP. Overexpression of catalase reduced the ...
  https://www.sigmaaldrich.com/catalog/papers/27607467
*  NIOSHTIC-2 Publications Search - 00163633 - Effects of organic solvent vehicles on benzo[a]pyrene metabolism in rabbit lung...
... metabolite profile were studied in lung microsomes prepared from male New Zealand white rabbits. The production of 3- OH-benzo( ... The authors suggest that DMSO is probably the best solvent vehicle for studying BP metabolism in rabbit lung microsomes. ... Effects of organic solvent vehicles on benzo[a]pyrene metabolism in rabbit lung microsomes.. ... metabolite profile were studied in lung microsomes prepared from male New Zealand white rabbits. The production of 3- OH-benzo( ...
  https://www.cdc.gov/niosh/nioshtic-2/00163633.html
*  3H]Benzo(a)pyrene Metabolism in Tracheal Epithelial Microsomes and Tracheal Organ Cultures | Cancer Research
3H]Benzo(a)pyrene Metabolism in Tracheal Epithelial Microsomes and Tracheal Organ Cultures. Marc J. Mass and David G. Kaufman ... Isolated tracheal microsomes catalyzed the binding of [3H]BP to DNA in vitro. Analysis of metabolites produced by tracheal ... 3H]Benzo(a)pyrene Metabolism in Tracheal Epithelial Microsomes and Tracheal Organ Cultures ... 3H]Benzo(a)pyrene Metabolism in Tracheal Epithelial Microsomes and Tracheal Organ Cultures ...
  http://cancerres.aacrjournals.org/content/38/11_Part_1/3861
*  JCI - Markedly inhibited 7-dehydrocholesterol-delta 7-reductase activity in liver microsomes from Smith-Lemli-Opitz homozygotes.
Markedly inhibited 7-dehydrocholesterol-delta 7-reductase activity in liver microsomes from Smith-Lemli-Opitz homozygotes.. S ... In Smith-Lemli-Opitz homozygotes, the transformation of 7-dehydrocholesterol to cholesterol by hepatic microsomes was blocked ... Thus, lathosterol 5-dehydrogenase is equally active which indicates that homozygotes liver microsomes are viable. Accordingly, ... 7-dehydrocholesterol into cholesterol by liver microsomes from seven controls and four Smith-Lemli-Opitz homozygous subjects. ...
  https://www.jci.org/articles/view/118223
*  Effect of Cardiotonic Lactones on Calcium Metabolism of Dog Cardiac Microsomes | Circulation Research
Effect of Cardiotonic Lactones on Calcium Metabolism of Dog Cardiac Microsomes. MARK L. ENTMAN, JOSEPH W. COOK, RUBIN BRESSLER ... Effect of Cardiotonic Lactones on Calcium Metabolism of Dog Cardiac Microsomes. MARK L. ENTMAN, JOSEPH W. COOK and RUBIN ... Effect of Cardiotonic Lactones on Calcium Metabolism of Dog Cardiac Microsomes. MARK L. ENTMAN, JOSEPH W. COOK and RUBIN ... The potency of the lactones in relation to calcium metabolism in the microsomes is influenced by ring saturation, position of ...
  http://circres.ahajournals.org/content/24/6/793
*  Phosphate binding by cerebral microsomes in relation to adenosine-triphosphatase activity | Biochemical Journal
Phosphate binding by cerebral microsomes in relation to adenosine-triphosphatase activity. R Rodnight, DA Hems, BE Lavin ... Phosphate binding by cerebral microsomes in relation to adenosine-triphosphatase activity Message Subject (Your Name) has ...
  http://www.biochemj.org/content/101/2/502
*  THE EFFECT OF SPIRONOLACTONE ON DRUG METABOLISM BY HEPATIC MICROSOMES | Journal of Pharmacology and Experimental Therapeutics
THE EFFECT OF SPIRONOLACTONE ON DRUG METABOLISM BY HEPATIC MICROSOMES. BITTEN STRIPP, MAYNARD E. HAMRICK, NICOLA G. ZAMPAGLIONE ... THE EFFECT OF SPIRONOLACTONE ON DRUG METABOLISM BY HEPATIC MICROSOMES. BITTEN STRIPP, MAYNARD E. HAMRICK, NICOLA G. ZAMPAGLIONE ... THE EFFECT OF SPIRONOLACTONE ON DRUG METABOLISM BY HEPATIC MICROSOMES. BITTEN STRIPP, MAYNARD E. HAMRICK, NICOLA G. ZAMPAGLIONE ... THE EFFECT OF SPIRONOLACTONE ON DRUG METABOLISM BY HEPATIC MICROSOMES Message Subject (Your Name) has forwarded a page to you ...
  http://jpet.aspetjournals.org/content/176/3/766
*  Response of Phospholipid Metabolism to Thiram in Rat Liver Microsomes | Springer for Research & Development
Incorporation of 14C into the phospholipid components of the liver microsomes isolated by a conventional method was determined ... Leyck S., Freundt K.J. (1978) Response of Phospholipid Metabolism to Thiram in Rat Liver Microsomes. In: Deutsche ... strongly with an inhibition of MFO as shown by a decrease in the O-demethylation of p-nitroanisol in prepared liver microsomes ...
  https://rd.springer.com/chapter/10.1007/978-3-662-39532-5_76
*  Metabolism of thioacetamide and thioacetamide S-oxide by rat liver microsomes. | Drug Metabolism & Disposition
Metabolism of thioacetamide and thioacetamide S-oxide by rat liver microsomes.. W R Porter and R A Neal ... Metabolism of thioacetamide and thioacetamide S-oxide by rat liver microsomes.. W R Porter and R A Neal ... Metabolism of thioacetamide and thioacetamide S-oxide by rat liver microsomes.. W R Porter and R A Neal ... Metabolism of thioacetamide and thioacetamide S-oxide by rat liver microsomes. Message Subject (Your Name) has forwarded a page ...
  http://dmd.aspetjournals.org/content/6/4/379
*  Effect of benzo(a)pyrene exposure on fluoranthene metabolism by mouse adipose tissue microsomes
... type of microsomes (hepatic microsomes of Vaca et al., 1992 versus adipose microsomes in our study), and exposure ... Microsomes from BaP-pretreated mice produced considerably higher proportion of FLA 2, 3-diol, and 2, 3 D FLA when microsomes ... as seen when microsomes from mice that have not received any BaP were exposed to FLA alone versus microsomes from mice that ... 8-diol when microsomes were incubated with BaP alone and BaP in combination with FLA when compared to microsomes from mice that ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2826120/
*  Propofol Hydroxylation by Dog Liver Microsomes: Assay Development and Dog Breed Differences | Drug Metabolism & Disposition
... in greyhound microsomes (1.7 ± 0.4 nmol/mg/min) compared with beagle microsomes (5.1 ± 1.3 nmol/mg/min) but was not ... Liver Microsomes.. The liver tissue used in this study was obtained from mature male greyhound, beagle, and mixed-breed dogs ( ... Liver microsomes were diluted with phosphate buffer and 50 μl was added to each tube. Next, 100 μl of an NADPH-regenerating ... Propofol Hydroxylation by Dog Liver Microsomes: Assay Development and Dog Breed Differences. Michael H. Court, Bonnie L. Hay- ...
  http://dmd.aspetjournals.org/content/27/11/1293
*  Kinetics of naphthalene metabolism in target and non-target tissues of rodents and in nasal and airway microsomes from the...
60 APPLIED LIFE SCIENCES; ENZYMES; EPITHELIUM; GLUTATHIONE CONJUGATES; MACACUS; METABOLISM; MICE; MICROSOMES; NAPHTHALENE; NOSE ... Title: Kinetics of naphthalene metabolism in target and non-target tissues of rodents and in nasal and airway microsomes from ... The catalytic efficiencies of microsomes from known susceptible tissues/subcompartments are 10 and 250 fold higher than in rat ... Kinetics of naphthalene metabolism in target and non-target tissues of rodents and in nasal and airway microsomes from the ...
  https://www.osti.gov/scitech/biblio/22285335-kinetics-naphthalene-metabolism-target-non-target-tissues-rodents-nasal-airway-microsomes-from-rhesus-monkey
*  Determination of the inhibitory potential of 6 fluoroquinolones on CYP1A2 and CYP2C9 in human liver microsomes - ZHANG - 2008 -...
If you are a society or association member and require assistance with obtaining online access instructions please contact our Journal Customer Services team ...
  http://onlinelibrary.wiley.com/doi/10.1111/j.1745-7254.2008.00908.x/pdf
*  Microsome - Wikipedia
To get microsomes containing a specific CYP or for high amounts of active enzyme, microsomes are prepared from Sf9 insect cells ... In this way, cytochrome P450 in microsomes is concentrated and isolated. Microsomes have a reddish-brown color, due to the ... microsomes are not present in healthy, living cells. Microsomes can be concentrated and separated from other cellular debris by ... Researchers often select microsome lots based on the enzyme activity level of specific CYPs. Some lots are available to study ...
  https://en.wikipedia.org/wiki/Microsome
*  In Vitro Metabolism of 17-(Dimethylaminoethylamino)-17-demethoxygeldanamycin in Human Liver Microsomes | Drug Metabolism &...
human liver microsome. HPLC. high-performance liquid chromatography. LC-MS/MS. liquid chromatography-tandem mass spectrometry. ... In Vitro Metabolism of 17-(Dimethylaminoethylamino)-17-demethoxygeldanamycin in Human Liver Microsomes. Nan Zheng, Peng Zou, ... In Vitro Metabolism of 17-(Dimethylaminoethylamino)-17-demethoxygeldanamycin in Human Liver Microsomes. Nan Zheng, Peng Zou, ... In Vitro Metabolism of 17-(Dimethylaminoethylamino)-17-demethoxygeldanamycin in Human Liver Microsomes. Nan Zheng, Peng Zou, ...
  http://dmd.aspetjournals.org/content/39/4/627.short
*  Quantitation of Immunoglobulin Genes by Nucleic Acid Hybridization with RNA from Myeloma and Spleen Microsomes | The Journal of...
Quantitation of Immunoglobulin Genes by Nucleic Acid Hybridization with RNA from Myeloma and Spleen Microsomes. Ursula Storb ... It was shown that RNA extracted from microsomes contained a very limited number of RNA species and was essentially ... Hyperimmune spleen DNA was hybridized with radiolabeled RNA from spleen and myeloma microsomes in the presence of unlabeled ... Quantitation of Immunoglobulin Genes by Nucleic Acid Hybridization with RNA from Myeloma and Spleen Microsomes ...
  http://www.jimmunol.org/content/108/3/755
*  Analytical Study of Kallikrein and Kallikrein-Like Esterase Activity in Subfractions from Rat Kidney Cortex Microsomes and...
Analytical Study of Kallikrein and Kallikrein-Like Esterase Activity in Subfractions from Rat Kidney Cortex Microsomes and ... Analytical Study of Kallikrein and Kallikrein-Like Esterase Activity in Subfractions from Rat Kidney Cortex Microsomes and ...
  https://link.springer.com/chapter/10.1007/978-1-4684-5143-6_48
*  6 alpha-hydroxylation of taurochenodeoxycholic acid and lithocholic acid by CYP3A4 in human liver microsomes
... Araya, Z Uppsala ... Fourteen recombinant expressed cytochrome P450 (CYP) enzymes, human liver microsomes from different donors, and selective ...
  http://uu.diva-portal.org/smash/record.jsf?pid=diva2:112787
*  Identification of Cytochrome P450 Isoforms Involved in CitalopramN-Demethylation by Human Liver Microsomes | Journal of...
Assay with human liver microsomes.. The basic incubation medium contained 0.1 or 0.2 mg/ml microsomes, 0.5 mM NADP+, 2.0 mM ... The value for the putative EM microsomes was significantly (P , .05) greater than that for the PM microsomes. For CYP3A4, the ... Human liver microsomes.. Human liver samples (n = 14) were obtained, as excess material removed during surgery on the liver, ... Microsomes from human B lymphoblastoid cell lines expressing each of eight human P450 isoforms were examined in terms of the ...
  http://jpet.aspetjournals.org/content/280/2/927