*  Liver Microsomes, S9 Fractions and Cytosol | Thermo Fisher Scientific - JP
... microsomes & other liver subcellular fractionsLarge pooled lots for reproducible, long-term studiesFractions carefully isolated ... Table 1 - Relevant applications for liver subcellular fractions Metabolic Enzymes Liver Microsomes Liver S9 Fractions Liver ... Table 3 - Kinetic parameters for the GIBCO® 50 donor human liver microsome pool Isoform Metabolite Km(μM) Vmax (nmol/min/mg) ... Human microsome pools and subcellular fractions Subcellular fractions, derived from the endoplasmic reticulum of liver, contain ...
  https://www.thermofisher.com/jp/en/home/industrial/pharma-biopharma/drug-discovery-development/microsomes-s9-fractions-cytosol.html
*  JCI - Markedly inhibited 7-dehydrocholesterol-delta 7-reductase activity in liver microsomes from Smith-Lemli-Opitz homozygotes.
Markedly inhibited 7-dehydrocholesterol-delta 7-reductase activity in liver microsomes from Smith-Lemli-Opitz homozygotes.. S ... Thus, lathosterol 5-dehydrogenase is equally active which indicates that homozygotes liver microsomes are viable. Accordingly, ... 7-dehydrocholesterol into cholesterol by liver microsomes from seven controls and four Smith-Lemli-Opitz homozygous subjects. ... that is catalyzed by lathosterol-5-dehydrogenase was equally rapid in controls and homozygotes liver microsomes (120 +/- 8 vs ...
  https://www.jci.org/articles/view/118223
*  Response of Phospholipid Metabolism to Thiram in Rat Liver Microsomes | Springer for Research & Development
Incorporation of 14C into the phospholipid components of the liver microsomes isolated by a conventional method was determined ... Leyck S., Freundt K.J. (1978) Response of Phospholipid Metabolism to Thiram in Rat Liver Microsomes. In: Deutsche ... strongly with an inhibition of MFO as shown by a decrease in the O-demethylation of p-nitroanisol in prepared liver microsomes ...
  https://rd.springer.com/chapter/10.1007/978-3-662-39532-5_76
*  Metabolism of thioacetamide and thioacetamide S-oxide by rat liver microsomes. | Drug Metabolism & Disposition
Metabolism of thioacetamide and thioacetamide S-oxide by rat liver microsomes.. W R Porter and R A Neal ... Metabolism of thioacetamide and thioacetamide S-oxide by rat liver microsomes.. W R Porter and R A Neal ... Metabolism of thioacetamide and thioacetamide S-oxide by rat liver microsomes.. W R Porter and R A Neal ... Metabolism of thioacetamide and thioacetamide S-oxide by rat liver microsomes. Message Subject (Your Name) has forwarded a page ...
  http://dmd.aspetjournals.org/content/6/4/379
*  Determination of the inhibitory potential of 6 fluoroquinolones on CYP1A2 and CYP2C9 in human liver microsomes - ZHANG - 2008 -...
If you are a society or association member and require assistance with obtaining online access instructions please contact our Journal Customer Services team ...
  http://onlinelibrary.wiley.com/doi/10.1111/j.1745-7254.2008.00908.x/pdf
*  Propofol Hydroxylation by Dog Liver Microsomes: Assay Development and Dog Breed Differences | Drug Metabolism & Disposition
Liver Microsomes.. The liver tissue used in this study was obtained from mature male greyhound, beagle, and mixed-breed dogs ( ... Liver microsomes were diluted with phosphate buffer and 50 μl was added to each tube. Next, 100 μl of an NADPH-regenerating ... Propofol Hydroxylation by Dog Liver Microsomes: Assay Development and Dog Breed Differences. Michael H. Court, Bonnie L. Hay- ... Propofol Hydroxylation by Dog Liver Microsomes: Assay Development and Dog Breed Differences. Michael H. Court, Bonnie L. Hay- ...
  http://dmd.aspetjournals.org/content/27/11/1293
*  In Vitro Metabolism of 17-(Dimethylaminoethylamino)-17-demethoxygeldanamycin in Human Liver Microsomes | Drug Metabolism &...
human liver microsome. HPLC. high-performance liquid chromatography. LC-MS/MS. liquid chromatography-tandem mass spectrometry. ... In Vitro Metabolism of 17-(Dimethylaminoethylamino)-17-demethoxygeldanamycin in Human Liver Microsomes. Nan Zheng, Peng Zou, ... In Vitro Metabolism of 17-(Dimethylaminoethylamino)-17-demethoxygeldanamycin in Human Liver Microsomes. Nan Zheng, Peng Zou, ... In Vitro Metabolism of 17-(Dimethylaminoethylamino)-17-demethoxygeldanamycin in Human Liver Microsomes. Nan Zheng, Peng Zou, ...
  http://dmd.aspetjournals.org/content/39/4/627.short
*  6 alpha-hydroxylation of taurochenodeoxycholic acid and lithocholic acid by CYP3A4 in human liver microsomes
... Araya, Z Uppsala ... Fourteen recombinant expressed cytochrome P450 (CYP) enzymes, human liver microsomes from different donors, and selective ... bile acid; 6 alpha-hydroxylation; CYP3A4; human liver; BILE-ACIDS; PIG-LIVER; METABOLISM; URINE; CYTOCHROME-P-450; ... The aim of the present study was to identify the enzymes in human liver catalyzing hydroxylations of bile acids. ...
  http://uu.diva-portal.org/smash/record.jsf?pid=diva2:112787
*  AID 31223 - Inhibition of acyl coenzyme A:cholesterol acyltransferase (ACAT) activity in rat liver microsome - PubChem
BioAssay record AID 31223 submitted by ChEMBL: Inhibition of acyl coenzyme A:cholesterol acyltransferase (ACAT) activity in rat liver microsome.
  https://pubchem.ncbi.nlm.nih.gov/bioassay/31223
*  Identification of Cytochrome P450 Isoforms Involved in CitalopramN-Demethylation by Human Liver Microsomes | Journal of...
Human liver microsomes.. Human liver samples (n = 14) were obtained, as excess material removed during surgery on the liver, ... Assay with human liver microsomes.. The basic incubation medium contained 0.1 or 0.2 mg/ml microsomes, 0.5 mM NADP+, 2.0 mM ... in human liver microsomes.. The immunoinhibition of CIT N-demethylation was examined by preincubating human liver microsomal ... Kinetic profile of CIT N-demethylation in human liver microsomes.. Eadie-Hofstee plots for the N-demethylation of CIT (2.5-500 ...
  http://jpet.aspetjournals.org/content/280/2/927
*  Evaluation of metabolism dependent inhibition of CYP2B6 mediated bupropion hydroxylation in human liver microsomes by monoamine...
Evaluation of metabolism dependent inhibition of CYP2B6 mediated bupropion hydroxylation in human liver microsomes by monoamine ... Human liver microsomal CYP2B6 activities were investigated using bupropion hydroxylation as probe substrate marker. The results ... In conclusion, the results from this study demonstrated that MAO inhibitors can inactivate human liver microsomal CYP2B6. The ... study was to evaluate the metabolism dependent inhibition of CYP2B6 catalyzed bupropion hydroxylation in human liver microsomes ...
  http://www.sigmaaldrich.com/catalog/papers/25656918
*  Biotransformation of tirilazad in human: 1. Cytochrome P450 3A-mediated hydroxylation of tirilazad mesylate in human liver...
Cytochrome P450 3A-mediated hydroxylation of tirilazad mesylate in human liver microsomes.. L C Wienkers, R C Steenwyk, P E ... Cytochrome P450 3A-mediated hydroxylation of tirilazad mesylate in human liver microsomes.. L C Wienkers, R C Steenwyk, P E ... Cytochrome P450 3A-mediated hydroxylation of tirilazad mesylate in human liver microsomes.. L C Wienkers, R C Steenwyk, P E ... Cytochrome P450 3A-mediated hydroxylation of tirilazad mesylate in human liver microsomes. ...
  http://jpet.aspetjournals.org/content/277/2/982
*  Benzene Metabolism by Ethanol-, Acetone-, and Benzene-inducible Cytochrome P-450 (IIE1) in Rat and Rabbit Liver Microsomes |...
In the present investigation it was found that benzene was metabolized at a rate 20-65-fold higher in liver microsomes from ... The data indicate that benzene is metabolized mainly by the ethanol-inducible P-450 form in liver microsomes and that the ... Benzene Metabolism by Ethanol-, Acetone-, and Benzene-inducible Cytochrome P-450 (IIE1) in Rat and Rabbit Liver Microsomes. ... Benzene Metabolism by Ethanol-, Acetone-, and Benzene-inducible Cytochrome P-450 (IIE1) in Rat and Rabbit Liver Microsomes ...
  http://cancerres.aacrjournals.org/content/48/19/5387
*  Isoniazid is a mechanism-based inhibitor of cytochrome P450 1A2, 2A6, 2C19 and 3A4 isoforms in human liver microsomes.
... ... METHODS: Using pooled human liver microsomes, the in vitro inhibitory effects of isoniazid on CYP1A2 (phenacetin O-deethylation ... CYP2C19 and CYP3A4 in human liver microsomes. Co-administration of isoniazid and drugs that are primarily metabolised by these ...
  https://www.omicsonline.org/references/isoniazid-is-a-mechanismbased-inhibitor-of-cytochrome-p450-1a2-2a6-2c19-and-3a4-isoforms-in-human-liver-microsomes-1363160.html
*  AID 706454 - Metabolic stability in human liver microsomes assessed as compound remaining at 1 uM after 60 mins by HPLC-MS...
Metabolic stability in human liver microsomes assessed as compound remaining at 1 uM after 60 mins by HPLC-MS analysis. ...
  https://pubchem.ncbi.nlm.nih.gov/bioassay/706454
*  In Vitro Glucuronidation of Thyroxine and Triiodothyronine by Liver Microsomes and Recombinant Human UDP...
In liver microsomes, phenolic glucuronidation was the highest in mice for T4 and in rats for T3 and lowest in monkeys for both ... In human liver microsomes, phenolic glucuronide was formed in higher amounts than acyl glucuronide for T4; in contrast, the ... Alamethicin (50 μg/mg protein) was mixed with liver microsomes for use in incubations. T4 or T3 in 5 μl of methanol was added ... To evaluate the effect of pH on the formation of T4 glucuronides, T4 was incubated with human liver microsomes at pH 6.8 and ...
  http://dmd.aspetjournals.org/content/35/12/2203
*  Amlodipine Metabolism in Human Liver Microsomes and Roles of CYP3A4/5 in the Dihydropyridine Dehydrogenation | Drug Metabolism ...
human liver microsomes. LC. liquid chromatography. MH+. protonated molecular ions. MS. mass spectrometry. MSn. multiple stage ... Amlodipine Metabolism in Human Liver Microsomes and Roles of CYP3A4/5 in the Dihydropyridine Dehydrogenation. Yanlin Zhu, Fen ... Amlodipine Metabolism in Human Liver Microsomes and Roles of CYP3A4/5 in the Dihydropyridine Dehydrogenation ... in human liver microsomes (HLM), and to identify the cytochrome P450 (P450) enzyme(s) involved in the M9 formation. Liquid ...
  http://dmd.aspetjournals.org/content/42/2/245
*  Tentative Identification of Benzo[a]pyrene Metabolite-Nucleoside Complexes Produced in Vitro by Mouse Liver Microsomes |...
Tentative Identification of Benzo[a]pyrene Metabolite-Nucleoside Complexes Produced in Vitro by Mouse Liver Microsomes. OLAVI ... Tentative Identification of Benzo[a]pyrene Metabolite-Nucleoside Complexes Produced in Vitro by Mouse Liver Microsomes. OLAVI ... Tentative Identification of Benzo[a]pyrene Metabolite-Nucleoside Complexes Produced in Vitro by Mouse Liver Microsomes. OLAVI ... When [3H]benzo[a]pyrene is incubated in vitro together with deproteinized salmon sperm DNA, NADPH, and mouse liver microsomes, ...
  http://molpharm.aspetjournals.org/content/14/2/306
*  Contribution of CYP3A4, CYP2B6, and CYP2C9 Isoforms toN-Demethylation of Ketamine in Human Liver Microsomes | Drug Metabolism &...
Liver to plasma concentration ratio of ketamine was taken into consideration. Pooled human liver microsomes and human ... average content of CYP3A4 was 146 pmol/mg of human liver microsomes and that of CYP2C9 was 89 pmol/mg of human liver microsomes ... demethylation of ketamine in human liver microsomes. Our results confirmed that NK is the major metabolite in human liver ... The in vitro total intrinsic clearance of ketamine in human liver microsomes was 23.3 × 10−6 ml/min/pmol P450 and that of theN- ...
  http://dmd.aspetjournals.org/content/30/7/853
*  Novel Mechanism for Dehalogenation and Glutathione Conjugation of Dihalogenated Anilines in Human Liver Microsomes: Evidence...
Novel Mechanism for Dehalogenation and Glutathione Conjugation of Dihalogenated Anilines in Human Liver Microsomes: Evidence ... fluoro Novel Mechanism reactive metabolites Dihalogenated Anilines GSH thiol attacks Human Liver Microsomes Glutathione ... Novel Mechanism for Dehalogenation and Glutathione Conjugation of Dihalogenated Anilines in Human Liver Microsomes: Evidence ... dihalogenated anilines observed in human liver microsomes. Of particular interest were conjugates formed in which one of the ...
  https://figshare.com/articles/Novel_Mechanism_for_Dehalogenation_and_Glutathione_Conjugation_of_Dihalogenated_Anilines_in_Human_Liver_Microsomes_Evidence_for_i_ipso_i_Glutathione_Addition/2601655/1
*  Characterization of Substrate Specificity of Dog CYP1A2 Using CYP1A2-Deficient and Wild-Type Dog Liver Microsomes | Drug...
The metabolic profile in dog liver microsomes was also different from human liver microsomes, with the contribution of 13X to ... 1 mg/ml dog or human liver microsomes, and 0.8 mM NADPH. For human liver microsomes, inhibitory effects of 1 μM ANF were also ... 0.8 mg/ml dog or human liver microsomes, and 0.8 mM NADPH. For human liver microsomes, inhibitory effects of 1 μM ANF were also ... 0.5 mg/ml dog or human liver microsomes, and 0.8 mM NADPH. For human liver microsomes, inhibitory effects of 1 μM ANF, human ...
  http://dmd.aspetjournals.org/content/36/9/1903
*  Identification of Enzymes Responsible for Primary and Sequential Oxygenation Reactions of Capravirine in Human Liver Microsomes...
Louis, MO). Human liver microsomes (pooled from 14 donors) were prepared at Pfizer (Groton, CT). Human P450 Supersomes 1A2, 3A4 ... Antibody Inhibition. The anti-human CYP3A4 antibody (0.4 mg/ml) was first incubated with human liver microsomes (0.2 mg/ml) for ... The effect of ritonavir on the formation of capravirine metabolites in human liver microsomes is shown in Fig. 2. The formation ... The contribution of CYP3A4 to the total metabolism of capravirine in human liver microsomes was thus estimated to be ,90% (91.7 ...
  http://dmd.aspetjournals.org/content/34/11/1798
*  Cytochrome P450 inhibitors. Evaluation of specificities in the in vitrometabolism of therapeutic agents by human liver...
Evaluation of specificities in the in vitrometabolism of therapeutic agents by human liver microsomes.. D J Newton, R W Wang ... Evaluation of specificities in the in vitrometabolism of therapeutic agents by human liver microsomes.. D J Newton, R W Wang ... Evaluation of specificities in the in vitrometabolism of therapeutic agents by human liver microsomes.. D J Newton, R W Wang ... Evaluation of specificities in the in vitrometabolism of therapeutic agents by human liver microsomes. ...
  http://dmd.aspetjournals.org/content/23/1/154
*  CYP2B6, CYP3A4, AND CYP2C19 ARE RESPONSIBLE FOR THE IN VITRO N-DEMETHYLATION OF MEPERIDINE IN HUMAN LIVER MICROSOMES | Drug...
Inhibition Studies with Human Liver Microsomes and Recombinant P450 Enzymes. Pooled human liver microsomes (0.25 mg) or P450 ... N-Demethylation of Meperidine by Human Liver Microsomes. In the presence of NADPH, human liver microsomes metabolized ... Human Liver Microsomes. Human liver tissue was processed at St. Jude Children's Research Hospital, and was provided by the ... Correlation Studies in Human Liver Microsomes. Human liver microsomal preparations (0.25 mg) were incubated with meperidine (75 ...
  http://dmd.aspetjournals.org/content/32/9/930