*  Semliki forest virus capsid - Stock Image F006/9297 - Science Photo Library
Semliki forest virus capsid, molecular model. This virus, named for the forest in Uganda where it was identified, is spread by the bite of mosquitoes. It can infect both humans and animals. In viruses, the capsid is the protein shell that encloses the genetic material. A capsid consists of subunits called capsomeres that self-assemble to form the shell seen here. Here, this shell, approximately spherical in shape, has icosahedral symmetry. One of the functions of the capsid is to aid the transmission of the viral genetic material into host cells. - Stock Image F006/9297
294966285 - EP 0996737 A1 2000-05-03 - SEMLIKI FOREST VIRUS VECTORS FOR GENE TRANSFER INTO NON-ENDOTHELIAL CARDIOVASCULAR CELLS - [origin: WO9909192A1] The invention provides methods of transducing non-endothelial cardiovascular cells with a recombinant Togavirus vector, vector kits, and vectors.[origin: WO9909192A1] The invention provides methods of transducing non-endothelial cardiovascular cells with a recombinant Togavirus vector, vector kits, and vectors.
*  Semliki Forest virus - Wikipedia
The Semliki Forest virus was first isolated from mosquitoes in the Semliki Forest, Uganda by the Uganda Virus Research Institute in 1942 and described by Smithburn & Haddow. It is known to cause disease in animals including humans. It is an Alphavirus found in central, eastern, and southern Africa. The Semliki Forest virus is a positive-stranded RNA virus with a genome of approximately 13,000 base pairs which encodes nine proteins. The 5' two thirds of the genome encode four non-structural proteins concerned with RNA synthesis and the structural proteins are encoded in the 3' third. Of the structural proteins, the C proteins makes up the icosahedral capsid which is enveloped by a lipid bilayer, derived from the host cell. The outermost surface of the virus is almost entirely covered by ...
*  Semliki Forest virus | MSDSonline
PATHOGEN SAFETY DATA SHEET - INFECTIOUS SUBSTANCES. SECTION I - INFECTIOUS AGENT NAME: Semliki Forest Virus SYNONYM OR CROSS REFERENCE: SFV (1-3), Old World Alphavirus (4), Semliki Forest Fever (5) CHARACTERISTICS: Family Togaviridae, Genus Alphavirus (6, 7). Virions have an approximate diameter of 50-70 nm and contain one positive stranded RNA molecule. Genetic material is enveloped in an icosahedral nucleocapsid.. SECTION II - HAZARD IDENTIFICATION PATHOGENICITY/TOXICITY: Most infections caused by the Semliki Forest Virus (SFV) are asymptomatic or very mild (3). When symptoms are present they are mild. During the acute phase of infection they are indistinguishable from those of malaria, influenza, or other febrile illnesses (3, 8). When present, symptoms include headache, fever, myalgia and athrolgia. Rare symptoms of abdominal pain, ...
*  Viruses | Free Full-Text | Polyprotein Processing as a Determinant for in Vitro Activity of Semliki Forest Virus Replicase
Semliki Forest virus (SFV) is an arthropod-borne alphavirus that induces membrane invaginations (spherules) in host cells. These harbor the viral replication complexes (RC) that synthesize viral RNA. Alphaviruses have four replicase or nonstructural proteins (nsPs), nsP1-4, expressed as polyprotein P1234. An early RC, which synthesizes minus-strand RNA, is formed by the polyprotein P123 and the polymerase nsP4. Further proteolytic cleavage results in a late RC consisting of nsP1-4 and synthesizing plus strands. Here, we show that only the late RCs are highly active in RNA synthesis in vitro. Furthermore, we demonstrate that active RCs can be isolated from both virus-infected cells and cells transfected with the wild-type replicase in combination with a plasmid expressing a template RNA. When an uncleavable polyprotein P123 and polymerase nsP4 were expressed together with a template, high ...
*  Semliki forest virus expressing interleukin-12 induces antiviral and antitumoral responses in woodchucks with chronic viral...
Semliki forest virus expressing interleukin-12 induces antiviral and antitumoral responses in woodchucks with chronic viral hepatitis and hepatocellular carcinoma. Semliki forest virus expressing interleukin-12 induces antiviral and antitumoral responses in woodchucks with chronic viral hepatitis and hepatocellular carcinoma.
*  Alteration of the cytoplasmic domain of the membrane-spanning glycoprotein p62 of Semliki Forest virus does not affect its...
Expression of the Semliki Forest virus p62/E2 protein was studied in the polarized epithelial cell line Madin-Darby canine kidney (MDCK). After infection this transmembrane protein, together with the other spike subunit E1, accumulates at the basolateral surface of MDCK cells (Fuller, S. D., C.-H. von Bonsdorff, and K. Simons, 1985, EMBO (Eur. Mol. Biol. Organ.) J., 4:2475-2485). The cDNAs encoding truncated forms of the protein were used to stably transform MDCK cells to examine the role of subunit oligomerization (E1-E2) and the cytoplasmic domain of p62/E2 in directed transport to the basolateral surface. The biochemical characteristics and polarity of the expressed proteins were studied using cell monolayers grown on nitrocellulose filters. A wild-type form of p62/E2, in the absence of E1, and two forms having either 15 or 3 of the wild-type 31-amino acid carboxyl cytoplasmic domain were all localized to the basolateral surface. These ...
*  A short treatment of cells with the lanthanide ions La3+, Ce3+, Pr3+ or Nd3+ changes the cellular chemistry into a state in...
Alpha- and flaviviruses contain class II fusion proteins, which form ion-permeable pores in the target membrane during virus entry. The pores generated during entry of the alphavirus Semliki Forest virus have been shown previously to be blocked by lanthanide ions. Here, analyses of the influence of rare earth ions on the entry of the flaviviruses West Nile virus and Uganda S virus revealed an unexpected effect of lanthanide ions. The results showed that a 30 s treatment of cells with an appropriate lanthanide ion changed the cellular chemistry into a state in which the cells no longer supported the multiplication of flaviviruses. This change occurred in cells treated before, during or after infection, did not inhibit multiplication of Semliki Forest virus and did not ...
*  Revues à comité de lecture international(2002-2003) - [TIMC-IMAG]
2003 Bicout DJ and Sache I. Persistent Walk and Spore Dispersal Density Function. Phys. Rev. E. 2003, vol. 67, n° 0319131 - 0319137. Billecocq A, Coudrier D, Boué F, Combes B, Zeller H, Artois M and Bouloy M. Expression of the nucleoprotein of the Puumala virus from the recombinant Semliki Forest virus replicon : characterization and use as a potential diagnostic tool. Clinical and diagnostic laboratory immunology. 2003, vol. 10, n° 4, pp. 658-663. Hours M, Anzivino-Viricel L, Maître A, (...)
*  PKR acts early in infection to suppress Semliki Forest virus production and strongly enhances the type I interferon response
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*  Structure of the Complex Carbohydrate Chains in the Membrane Glycoproteins of Semliki Forest Virus | Biochemical Society...
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*  Pathogen Safety Data Sheets: Infectious Substances - Semliki Forest Virus - Canada.ca
These Pathogen Safety Data Sheets, regulated under Workplace Hazardous Materials Information System (WHMIS) legislation, are produced for personnel working in the life sciences as quick safety reference material relating to infectious micro-organisms.
*  Dr Chris Cane</span>
I read Natural Sciences at Cambridge before researching for a PhD in molecular virology at the National Institute for Medical Research in London. After a couple of post-docs at the University of Warwick working on Semliki Forest and influenza viruses, I moved to the University of Leicester for four years, first as a lecturer and later a research fellow in the Department of Biochemistry. I then spent four years in the MRC Toxicology Unit at Leicester working on antioxidants and their role in resistance to infection. Following nine years as senior lecturer in human biology at the University of Northampton, I returned to the University of Leicester to my present post as Director of Taught Postgraduate Programmes in the College of Medicine, Biological Sciences and Psychology.. I was awarded a University Teaching Fellowship in 2010 "in recognition of exceptional leadership and for the development in postgraduate studies in the College of Medicine, Biological ...
US Air Force Research Lab. Monday, April 24, 2017, Colloquia Auditorium. Electron microscopy has been one of the robust tools in characterizing the life cycle of viruses, such as Semliki Forest Virus (SFV), an alphavirus that belongs to Togavirus family and causes encephalitis in human. The viral particle of SFV is composed of a nucleocapsid enclosed by a lipid-anchored glycoprotein spikes and infects cell through clathrin-dependent endocytosis pathway where it releases the nucleocapsid into the cytoplasm after membrane fusion at the late endosome. Cryo-electron microscopy and single particle reconstruction revealed the structure of SFV virion that both the nucleocapsid and surface spikes adapted into icosahedral symmetry with T4 surface lattice and that the connection between nucleocapsid protein and C-terminal tail of the glycoprotein stabilizes the particle integrity. ...
*  Tattoo Delivery of a Semliki Forest Virus-Based Vaccine Encoding Human Papillomavirus E6 and E7. | Sigma-Aldrich
Sigma-Aldrich offers abstracts and full-text articles by [Stephanie van de Wall, Mateusz Walczak, Nienke van Rooij, Baukje-Nynke Hoogeboom, Tjarko Meijerhof, Hans W Nijman, Toos Daemen].
*  SGDB | Show Synthetic Gene ID: 260
We have recently shown that the NS3-based genetic immunogens should contain also hepatitis C virus (HCV) nonstructural (NS) 4A to utilize fully the immunogenicity of NS3. The next step was to try to enhance immunogenicity by modifying translation or mRNA synthesis. To enhance translation efficiency, a synthetic NS3/4A-based DNA (coNS3/4A-DNA) vaccine was generated in which the codon usage was optimized (co) for human cells. In a second approach, expression of the wild-type (wt) NS3/4A gene was enhanced by mRNA amplification using the Semliki forest virus (SFV) replicon (wtNS3/4A-SFV). Transient tranfections of human HepG2 cells showed that the coNS3/4A gene gave 11-fold higher levels of NS3 as compared to the wtNS3/4A gene when using the CMV promoter. We have previously shown that the presence of NS4A enhances the expression by SFV. Both codon optimization and mRNA amplification resulted in an improved immunogenicity as evidenced by higher ...
*  "Immunoglobulin synthesis in nude (nu/nu), nu/+ and reconstituted nu/nu" by A J. Suckling, S Jagelman et al.
Suckling, A J.; Jagelman, S; and Webb, H E., "Immunoglobulin synthesis in nude (nu/nu), nu/+ and reconstituted nu/nu mice infected with a demyelinating strain of semliki forest virus." (1982). Subject Strain Bibliography 1982. 3033 ...
*  Protein folding during cotranslational translocation - Research Collection
*  EP 0982405 A3 20000412 - Recombinant alphavirus vectors
387500898 - EP 0982405 A3 2000-04-12 - Recombinant alphavirus vectors - [origin: WO9507994A2] The present invention provides composition and methods for utilizing recombinant alphavirus vectors.[origin: WO9507994A2] The present invention provides composition and methods for utilizing recombinant alphavirus vectors.
*  Boosting immunity with whole foods | The Chronicle Herald
As we fall back into the cooler months, many people are dreading cold and flu season. Whether or not you have children who have started back to school, the spread of bacteria and viruses spikes this time every year. How can we avoid sick days and stay well during the fall and winter months?
*  What inspired you to make this poem? I know the forest... but how we're you inspired? Did you...
Listing of the answers to the question: What inspired you to make this poem? I know the forest... but how we're you inspired? Did you... visit a forest somewhere? If so, which forest did you go to? Or did you read something on forests? Etc.
*  Di t ticien Forest (1190) - Liste des Di t ticiens Forest
Pour trouver un Di t ticien sur Forest (1190), cliquez sur cette page de l'annuaire pro Allo-bruxelles.be qui liste les Di t ticiens Forest (1190).
*  Treasured Forest | Retty's Life Journey
One thing that I loved the most from my journey to Bali was the forest. I remember a friend in France asked me to visit a small forest near her house. I was astounded to see the trees that she was proudly claiming as a forest. It was even far from the Indonesian's Bogor Botanical…
*  suggestions for a food forest for me? (forest garden forum at permies)
i have an area that i want to make into a food forest and in the more open part i want to add hugel beds perhaps. i would like to do mostly just regul