Adenovirus-Mediated Overexpression of c-Jun and c-Fos Induces Intercellular Adhesion Molecule-1 and Monocyte Chemoattractant...
Fos-associated protein p39 is the product of the jun proto-oncogene. Science. 1988;240:1010-1016. ... Protein Extraction and Western Blot Analysis. Nuclear proteins were extracted from HUVECs as previously described.12 Protein ... The Jun-Fos complex is the major component of cellular AP-1.19 20 To induce AP-1 activation specifically, a straightforward ... Methods. Adenoviral Vectors. To construct the recombinant adenoviruses expressing human c-jun (AdJun) and c-fos (AdFos) genes, ...http://atvb.ahajournals.org/content/19/9/2078
... not only were the cellular p65 levels decreased effectively, but the RON protein levels were also decreased, and this decrease ... protein kinase C, protamine kinase, pp60c-src tyrosine kinase, p44/42 mitogen-activated protein kinase, and c-Jun-NH2-kinase ( ... Oligonucleotide primers specific for the RON promoter spanning the p65-binding sites (forward, 5′-CTC CAA GGG CCG GAA GAG TCG ... Materials and Methods. Cells, treatment, and reagents. MDA-MB-231 and MDA-MB-468 breast carcinoma cells were cultured as ...http://cancerres.aacrjournals.org/content/68/13/5185
Anti-fibrotic effects of phenolic compounds on pancreatic stellate cells | BMC Complementary and Alternative Medicine | Full...
... curcumin and resveratrol significantly suppressed the mRNA and protein levels of several fibrotic mediators namely alpha-smooth ... We examined the anti-fibrotic effects of a variety of herbal constituents using a cellular platform, the LTC-14 cells, which ... Methods. We examined the anti-fibrotic effects of a variety of herbal constituents using a cellular platform, the LTC-14 cells ... Extracted nuclear and cytoplasmic proteins were subjected to Western blotting analysis. Protein levels of α-SMA in the ...https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-015-0789-y
An equal amount of protein was subjected to SDS-PAGE. Proteins were transferred to polyvinylidene difluoride membranes and ... The siRNAs targeting the human GSK-3β gene (5′-AAG AAT CGA GAG CTC CAG ATC-3′, designated as #4 and 5′-AAG TAA TCC ACC TCT GGC ... Materials and Methods. Cells and Reagents. The human prostate cancer cell lines (LNCaP, DU145, and PC-3) and HEK293 cells were ... recent evidences demonstrated that GSK-3β can be activated in response to certain cellular stimuli, and its activity is ...http://mct.aacrjournals.org/content/2/11/1215
Patente US6025194 - Nucleic acid sequence of senescence asssociated gene - Google Patentes
... as well as peptides and antibodies corresponding to the GC6 protein can be used to identify senescent cells, distinguish ... Isolated, purified, and recombinant nucleic acids and proteins corresponding to the human GC6 gene and its mRNA and protein ... The present invention relates to cellular senescence, markers of cellular senescence, and methods and reagents for identifying ... CAG TCT GAG GGT CAC TGC ACT TTG GAG TGC CT - #G GAA GAG GCT CTGGAA 1148 Gln Ser Glu Gly His Cys Thr Leu Glu Cys Le - #u Glu Glu ...http://www.google.es/patents/US6025194
... protein-protein interactions, and stability of transcription factors but also to other cellular proteins by acetylation (3). ... Protein was quantified by the Bradford method, and equal amounts of protein were loaded onto 10% Gelatin-Zymograms (Invitrogen ... CREB-binding protein/p300 are transcriptional coactivators of p65. Proc Natl Acad Sci U S A 1997;94:2927-32. ... GGG GTT TGT GCC ACT GGT GAG-3′; reverse: 5′-GGG AGG CAA TTC TCC ATG GTT C-3′. Values were normalized to input controls taken ...http://mct.aacrjournals.org/content/10/9/1644
Protocols and Video Articles Authored by Gaoqing Yang
... identified proteins were used to infer an overall interaction map linking the ORF3 protein with components of the host cellular ... While the sequence of the decorin core protein is determined by the gene encoding its structure, the structure of its GAG chain ... We apply our method to simulated datasets and achieve higher accuracy compared to CNVnator. Moreover, we apply our method to ... Jun, 2013 , Pubmed ID: 23423786 GATA-binding protein 2 (GATA2) is a nuclear transcription factor that plays a critical role in ...https://www.jove.com/author/Gaoqing_Yang
US5962415A - Compositions comprising a peptide inhibitor of nuclear protein translocation and an immunosuppressant and methods...
Novel compositions of immunosuppressants, such as cyclosporin, and polypeptide inhibitors of cytoplasmic protein nuclear ... and polypeptide inhibitors of nuclear translocation of cellular proteins have been disclosed. Although preferred embodiments of ... In unstimulated cells, a major form of NF-κB is a heterodimer of p50 and p65 (RelA) subunits. Nonactive NF-κB is retained in ... 6C is a photograph of a gel depicting the effect of HIV-1MEM polypeptide on the expression of proviral gag sequences in anti- ...https://patents.google.com/patent/US5962415?oq=ascentive
Protocols and Video Articles Authored by Jing Huang (Translated to Portuguese)
CHARMM36m: an Improved Force Field for Folded and Intrinsically Disordered Proteins Nature Methods. Jan, 2017 , Pubmed ID: ... inhibitor Icotinib by analyzing protein expression and amplification of EGFR proto-oncogene in esophageal squamous cell ... The main cellular fatty acids included summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), iso-C15 : 0, C16 : 0, iso-C17 : 0 ... B. Jun, 2016 , Pubmed ID: 27299801 We have studied the structure of (Ala)5, a model unfolded peptide, using a combination of 2D ...https://www.jove.com/author/Jing_Huang?language=Portuguese
AKT3 AKT serine/threonine kinase 3 [Homo sapiens (human)] - Gene - NCBI
RAC-gamma serine/threonine protein kinase. v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma). NP_ ... organism-specific biosystemThe transit of proteins and other cargo through the cell requires a cellular transport process in ... gag HIV-1 p17 induces capillary-like structures in human endothelial cells by binding to CXCR1 and CXCR2, which requires ... HIV-1 Vpr-induced upregulation of CCL5 requires p50 and p65 subunits of NF-kappaB, p38delta MAPK, Akt-2 and Akt-3, and AP-1 ...https://www.ncbi.nlm.nih.gov/gene/10000
Other | Discovery of Brain-Penetrant, Irreversible Kynurenine Aminotransferase II Inhibitors
21 Myc protein take part in oncogenesis in a multitude of tumors by a genuine variety of different systems, including raising ... p65), RelB, NF-B1 (p50/p105), and NF-B2 (p52/p100).31 Currently, a couple of zero spontaneous animal choices for MCL, although ... GAGs) such as for example hyaluronic acidity (HA) heparan sulfate (HS) and chondroitin sulfate can be found in the vitreous gel ... The proto-oncogene c-kit, localized to human chromosome 4, encodes a transmembrane receptor, CD117/c-kit, belonging to the ...http://researchreport-digital.com/category/other/
Inhibition of Elastase-Pulmonary Emphysema in Dominant-Negative MafB Transgenic Mice
... and 10 ng of nuclear protein was used to assess the activity of NF-kB p65 and AP-1, c-fos, using TransAM® NFκB Family ... also known as V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, is a member of the Maf protein family and possesses a ... GCC TAC AAG GTC AAG TGC GAG AAA CTC GCC; MafB sh-RNA antisense, GGC GAG TTT CTC GCA CTT GAC CTT GTA GGC; control sh-RNA sense, ... Chronic obstructive pulmonary disease: molecular and cellular mechanisms. Eur Respir J. 2003;22:672-88 ...http://www.ijbs.com/v10p0882.htm
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Here we describe a protein purification method for nucleotide-binding leucine-rich repeat (NB-LRR)-interacting proteins and the ... Neuroscience, Issue 39, Cellular Biology, Aptamer, RNA, amyloid β-protein, oligomer, amyloid fibrils, protein assembly ... nuclear translocation of the p65 subunit of NF-κB (p65), and secretion of the hallmark proinflammatory cytokine, tumor necrosis ... Yukiko Doi, Hideyuki Takeuchi, Hiroyuki Mizoguchi, Kazuya Fukumoto, Hiroshi Horiuchi, Shijie Jin, Jun Kawanokuchi, Bijay ...https://www.jove.com/visualize/abstract/25062013/granulocyte-colony-stimulating-factor-attenuates-oligomeric-amyloid
Brain Infection by Neuroinvasive but Avirulent Murine Oncornaviruses - PDF
Rabbit Polyclonal antibody to NFkB p65 (v-rel reticuloendotheliosis viral oncogene homolog A (avian)) Datasheet GeneTex, Inc : ... proteins in granule neurons implies that there are differences in the ways in which these viral proteins interact with cellular ... The DNA -, RNA -, Protein Pathway The DNA -, RNA -, Protein Pathway RNA Polymerase = enzyme that makes mrna from the DNA gene ... Diagnostic Methods of HBV and HDV infections Diagnostic Methods of HBV and HDV infections Zohreh Sharifi,ph.D Blood Transfusion ...http://healthdocbox.com/Aids_and_Hiv/65517162-Brain-infection-by-neuroinvasive-but-avirulent-murine-oncornaviruses.html
Sensitivity of leukemic T-cell lines to arsenic trioxide cytotoxicity is dependent on the induction of phosphatase B220/CD45R...
Cellular redox status is not essential for As2O3 sensitivity of leukemic T cells, suggesting the existence of additional ... The protein from nuclear extracts was quantified by the Bradford method (Bio-Rad, France). An equal amount of nuclear extract ( ... Takayama S, Reed JC, Homma S: Heat-shock proteins as regulators of apoptosis. Oncogene. 2003, 22: 9041-9047. 10.1038/sj.onc. ... Cellular redox status is not essential for As2O3 sensitivity of leukemic T cells, suggesting the existence of additional ...https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-251
October 2016 - New β-secretase inhibitors for treatment of Alzheimer's disease
Cellular behavior can be affected not merely by the quantity of adsorbed proteins but also the orientation and conformation ... conflicting arguments concerning HTLV-1 manifestation might therefore continue until more sensitive protein detection methods ... Cdx2 can be able to connect to the p65 subunit of NFκB and for that reason avoid the NFκB binding and activation from the Cox-2 ... mRNA had been increased as soon as 4 h after initiation of lifestyle and peaked at 8 h accompanied by a rise in Env Gag/Pol and ...http://healthyguide.info/2016/10/