Bioline International Official Site (site up-dated regularly)
Random amplified polymorphic DNA of mosquito species and populations (Diptera: Culicidae): Techniques, statistical analysis, ... Materials and Methods. Identification - Peyton et al. (1992) provide a definition of the subgenus Nyssorhynchus. Species in the ... Use of the random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) to detect DNA polymorphisms in aphids ( ... Applications of random amplified polymorphic DNA (RAPD) in molecular ecology. Mol Ecol 1: 55-63.. Kambhampati S, Black WC IV, ...http://www.bioline.org.br/request?oc95147
Genetic analysis of Chrysanthemum hybrids based on RAPD molecular markers
An alternative technique for identifying molecular marks called random amplified polymorphic DNA (RAPD) has been developed ( ... In this method, by using a single arbitrary primer (10 mer) and amplifying DNA by polymerase chain reaction (PCR), the ... Variability of the random amplified polymorphic DNA assay among thermal cyclers, and effects of primer and DNA concentration. ... Random amplified polymorphic DNA markers for DNA fingerprinting and genetic variability assessment of minute parasitic wasp ...http://ejournal.sinica.edu.tw/bbas/content/2000/4/bot414-01.html
Topic: Seeds Induced to Germinate Rapidly by Mentally Projected `Qi Energy Are Apparently Genetically Altered
RAPD (random amplified polymorphic DNA) is a molecular marking technique developed by two teams, Williams et al. (1990) and ... The RAPD method was used to compare treated groups of wheat and pea seeds and their controls using 11 selected primers. Seven ... Differences among the genomes are revealed by the presence or absence of certain DNA fragments that are amplified, the DNA ... Primer pairs were enzymatically amplified from 10-50 ng of wheat or pea DNA in a 20 [micro]l reaction. Reactions contained 10 ...https://healingtaousa.com/topic/seeds-induced-to-germinate-rapidly-by-mentally-projected-qi-energy%C2%92-are-apparently-genetically-altered/
Genetic diversity of the sunflower caterpillar (Chlosyne lacinia saundersii Doubleday and Hewitson) (Lepidoptera: Nymphalidae)...
Random Amplified Polymorphic DNA), as it identifies the DNA polymorphism amplified at random through the use of a sole ... Although this technique results in amplification of random sequences, several authors have demonstrated its efficiency to ... The comparison of den-dograms by objective methods. Taxon 11: 30-40. [ Links ]. ... DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18: 6531-6535. [ Links ]. ...http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000400029
The utilization of micro-and macromolecular data for the analyses of evolutionary problems : examples from Juniperus and...
The development of the random amplified polymorphic DNA (RAPD) markers (Williams et al., 1990; Welsh et al., 1991) allowed ... In the last decade, techniques based on DNA markers have been used to detect variation at DNA level and have proven to be ... The basics of the methods employed will be described briefly and their applications also reviewed. ... The RAPD technique has been useful in studying polymorphism, identifying genes of interest, and permitting the determination of ...https://www.duo.uio.no/handle/10852/12094
Assessment of Genetic Diversity in Podophyllum peltatum by Molecular Markers
RAPD (random amplified polymorphic DNA) and AFLP (amplified fragment length polymorphism) are genetic fingerprinting techniques ... One method that is relatively accurate and reproducible from lab to lab is AFLP. This technique requires that the DNA is first ... DNA fingerprinting of strawberry (Fragaria × ananassa) cultivars using randomly amplified polymorphic DNA (RAPD) markers. ... Amplified DNA Marker Scoring. Each genomic DNA sample was scored for the presence or absence of specific PCR bands (DNA marker ...https://hort.purdue.edu/newcrop/ncnu02/v5-537.html
Genetic analysis of the cultivated potato Solanum tuberosum L. Phureja Group using RAPDs and nuclear SSRs | SpringerLink
Tinker NA, Fortin MG, Mather DE (1993) Random amplified polymorphic DNA and pedigree relationships in spring barley. Theor Appl ... a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407-4414PubMedGoogle Scholar ... Wieczorek J, Guo Q, Hijmans RJ (2004) The point-radius method for georeferencing locality descriptions and calculating ... This nuclear simple sequence repeat (nSSR or microsatellite) study complements a prior random amplified polymorphic DNA (RAPD) ...https://link.springer.com/article/10.1007%2Fs00122-006-0399-7
Skewed RAPD markers in linkage maps of Citrus
Among these, random amplified polymorphic DNA (RAPD) markers are the most common because the technique is easy, inexpensive, ... Material and Methods. The species used were Citrus reticulata Blanco cv. Cravo, C. sinensis (L.) Osbeck cv. 'Pêra' and 94 F1 ... Cai Q, Guy CL and Moore GA (1994) Extension of the linkage map in Citrus using random amplified polymorphic DNA (RAPD) markers ... DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531-6535. [ Links ]. ...http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300021&lng=en&nrm=iso&tlng=en
Molecular Strategies: Detection of Foodborne Bacterial Pathogens | Springer for Research & Development
Conventional methods of pathogen identification have often depended on the identification of disease symptoms, isolation, and ... Comparison of amplified ribosomal DNA restriction analysis, random fragment polymorphic DNA analysis, and amplified fragment ... Evaluation of the DNA fingerprinting method AFLP as a new tool in bacterial taxonomy. Microbiology 142: 1881-1893.CrossRef ... A PCR-based DNA fingerprinting technique: AFLP for molecular typing of bacteria. Nucleic Acids Res. 18: 3649-3650.CrossRef ...https://rd.springer.com/chapter/10.1007/978-1-4419-7931-5_8
Patent US7709704 - Cotton variety 04T048 - Google Patents
... cotton plants by crossing the cotton variety 04T048 with itself or another cotton variety and plants produced by such methods. ... Still further provided by the invention are methods for producing ... Randomly Amplified Polymorphic DNAs (RAPDs), DNA Amplification Fingerprinting (DAF), Sequence Characterized Amplified Regions ( ... In the method, the plant breeding techniques may be selected from the group consisting of recurrent selection, mass selection, ...http://www.google.com/patents/US7709704?dq=%22melissa+white%22
Synthetic Consolidants Attacked by Melanin-Producing Fungi: Case Study of the Biodeterioration of Milan (Italy) Cathedral...
... these methods include restriction fragment length polymorphism analysis (3, 10, 30, 31), random amplified polymorphic DNA ... Immunostaining technique. In order to obtain rapid identification and localization of melanin-producing fungi with a method ... strains isolated from rocks and other habitats assessed by physiological methods and by random amplified polymorphic DNA (RAPD ... a method independent from cultivation. Total DNA was extracted from samples pulverized in a mortar following a method ...http://pubmedcentralcanada.ca/pmcc/articles/PMC1797126/?lang=en-ca
Características morfológicas y genéticas permiten la identificación de cultivares de ajo en las regiones Norte y Central de...
... has been characterized by the random amplifica-tion of polymorphic DNA fragment technique (RAPD). This characteriza-tion has ... With the amplified DNA fragments, a binary matrix was generated; afterwards, a dissimilarity matrix and dendrogram were ... MATERIALS AND METHODS. The following varieties were used in this study: Coreano, California, Chino, Español, Cortazar, Positas ... DNA extraction and RAPD analysis. Genomic DNA was extracted from frozen leaves according to Doyle & Doyle (1990). DNA samples ...http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572008000100007&lng=es&nrm=iso&tlng=en
Molecular characterization and classification of Trypanosoma spp. Venezuelan isolates based on microsatellite markers and...
At a molecular level, T. evansi is characterized by the complete loss of the maxicircles of the kinetoplastic DNA, while T. ... by random amplified polymorphic DNA method. Vet. Parasitol. 2009; doi:10.1016/j.vetpar.2009.01.034 ... A novel, high-throughput technique for species identification reveals a new species of tsetse-transmitted trypanosome related ... Methods. Trypanosoma spp. field isolates and DNA extraction Nine Venezuelan Trypanosoma spp. isolates were obtained from horse ...https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-015-1129-2
DNA flow cytometry enabled ploidy and the genome size of Musa genotypes to be determined and was also used to monitor ... Screening techniques were developed to obtain disease (Fusarium wilt, black Sigatoka) and nematode resistant plants, while ... entitled Cellular biology and biotechnology including mutation techniques for creation of new useful banana genotypes . In 1996 ... Random amplified polymorphic DNA (RAPD). Random amplified polymorphic DNA (RAPD) is a novel technique developed by Williams et ...http://www.fao.org/docrep/007/ae216e/ae216e07.htm
Genetic diversity and structure of Jatropha curcas L. in its centre of origin
Additionally, the marker systems used have been mainly dominant, such as random amplified polymorphic DNA (RAPD), amplified ... The genomic DNA was isolated and purified by the method described by Doyle and Doyle (1990) modified for processing samples of ... due to the propagation technique used by Mesoamerican Jatropha farmers. Other researchers raised similar ideas to explain the ... genotypes using randomly amplified polymorphic DNA markers. Karnataka Journal of Agricultural Sciences 22: 293-295. ...https://www.cambridge.org/core/journals/plant-genetic-resources/article/genetic-diversity-and-structure-of-jatropha-curcas-l-in-its-centre-of-origin/6D179B4FA89126417D6105C336AFB277/core-reader
ScopeMed.org - Deposit for Medical Articles
Genetic Analysis of Escherichia coli Strains Isolated From Patients With Spinal Cord Injury by Random Amplified Polymorphic DNA ... EXTERNAL DEBT IN DEVELOPING COUNTRIES AND DEBT REDUCTION TECHNIQUES IN 1990's. Seyfettin ERDOGAN, Vedat CENG Z. Ekonomik ... RAPD) Method. Dr. Abdullah KILI , Dr. Mehmet YAPAR, Dr. Mehmet Ali SARA LI, Dr. Mehmet BAYSALLAR, Dr. Levent DO ANCI. Gulhane ...http://www.scopemed.org/?term=Mehmet%20Ozg%C3%BCr%20Erdogan&sarea=author&b1=Search+ScopeMed
Publications: O'Brien, Philip - Murdoch Research Repository
1995) Characterization of cereal bare patch isolates of Rhizoctonia solani by random amplified polymorphic DNA analysis. Plant ... 1993) Improved method for protoplast regeneration of rhizoctonia solani. Soil Biology and Biochemistry, 25 (5). pp. 633-636. ... 1991) An improved technique for fluorescence staining of fungal nuclei and septa. Australasian Plant Pathology, 20 (3). pp. 119 ... 1993) Analysis of variation in isolates of Rhizoctonia solani by random amplified polymorphic DNA assay. Mycological Research, ...http://researchrepository.murdoch.edu.au/view/author/O=27Brien,_Philip.date.html
The American Journal of Tropical Medicine and Hygiene | An Assessment of the Genetic Diversity of Leishmania infantum Isolates...
In fact, statistical analyses found no significant differences between the isolates using both random amplified polymorphic DNA ... and multilocus microsatellite typing genotyping techniques with three and seven markers, respectively. These findings provide ... Features of DNA fragments obtained by random amplified polymorphic DNA (RAPD) assays. Mol Ecol 8: 493- 503.[Crossref]. ... Inferring phylogenies from protein sequences by parsimony, distance, and likelihood methods. Methods Enzymol 266: 418- 427.[ ...http://www.ajtmh.org/content/journals/10.4269/ajtmh.2012.11-0300
Genetic relationships (RAPD-PCR) between geographically separated populations of the 'cosmopolitan' interstitial polychaete...
Identification of grapewine cultivars by DNA analyses: pitfalls of random amplified polymorphic DNA techniques using 10mer ... Total DNA was isolated by a modification of the method of Kocher et al. (1989). The DNA was extracted from frozen tissues (-70 ... Application of random amplified polymorphic DNA (RAPD) in molecular ecology. Mol. Ecol. 1: 55-63. Hagerman, G. M., and R. M. ... Effects of thermocyclers and primers on the reproducibility of banding patterns in random amplified polymorphic DNA analysis. ...https://www.thefreelibrary.com/Genetic+relationships+
ASMscience | Molecular Epidemiology of Extraintestinal Pathogenic |span class='jp-italic'|Escherichia coli|/span
Random Amplified Polymorphic DNA. 0.44747096. Restriction Fragment Length Polymorphism. 0.44747096. Random Amplified ... Fingerprinting microbial genomes using the RAPD or AP-PCR method. Methods Mol Cell Biol 5:13-24.. ... New molecular techniques for microbial epidemiology and the diagnosis of infectious diseases. J Infect Dis 161:595-602.[PubMed] ... Polymorphic DNA. 0.44747096. Restriction Fragment Length Polymorphism. 0.44747096. Outer Membrane Protein A. 0.43003705. Type 1 ...http://www.asmscience.org/content/journal/ecosalplus/10.1128/ecosalplus.126.96.36.199
Start Codon Targeted (SCoT) Polymorphism: A Simple, Novel DNA Marker Technique for Generating Gene-Targeted Markers in Plants |...
Random amplified polymorphic DNA (RAPD) markers have been used for numerous applications in plant molecular genetics research ... Random amplified polymorphic DNA (RAPD) markers have been used for numerous applications in plant molecular genetics research ... A novel method for generating plant DNA markers was developed based on the short conserved region flanking the ATG start codon ... AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 1995;23:4407-14.PubMedCrossRefGoogle Scholar ...https://link.springer.com/article/10.1007%2Fs11105-008-0060-5
ASSESSMENT OF GENETIC DIVERSITY WITHIN GERMPLASM ACCESSIONS IN. - Free Online Library
For Molecular characterizationRAPD (Random Amplified Polymorphic DNA) analysis selected for the estimation of genetic diversity ... DNA extraction method: Total genomic DNA was isolated from fresh and healthy leaves using the CTAB (hexadecyltrimethylammonium- ... The RAPD is the first PCR-based molecular markers technique to develop DNA marker for detecting and monitoring pedigree ... and random amplified polymorphic DNA (RAPD) (Bredemeijer et al., 1998, Villand et al., 1998, Park et al., 2004 and Garcia- ...https://www.thefreelibrary.com/ASSESSMENT+OF+GENETIC+DIVERSITY+WITHIN+GERMPLASM+ACCESSIONS+IN-a0351034665
US7229810B2 - Polymer conjugates of proteinases - Google Patents
Methods are provided for the stabilization of proteinases by the covalent attachment of or admixture with water-soluble ... as well as more complex PCR-based nucleic acid fingerprinting techniques such as Random Amplified Polymorphic DNA ("RAPD") ... In additional embodiments, the invention provides methods of synthesizing or amplifying nucleic acid molecules. Methods ... In certain such methods, the synthesized (i.e., amplified) nucleic acid molecule may be further amplified prior to insertion ...https://patents.google.com/patent/US7229810?oq=6437692
Detecting Seeds of Small Broomrape with PCR
... researchers have turned to molecular methods, in particular the randomly amplified polymorphic DNA (RAPD) technique, to ... Use of random amplified polymorphic DNA (RAPD) markers in the study of the parasitic weed Orobanche. Theor Appl Gen 93:367-372. ... The PCR amplified small broomrape DNA and did not amplify DNA from other Orobanche species with similar host ranges found in ... 5). The primer pair did not amplify DNA from O. californica Cham. & Schldl. and O. fasciculata Nutt., two other Orobanche spp. ...http://www.plantmanagementnetwork.org/pub/php/research/broomrape/