Surprisingly, the metabolism of the host cells, as judged by the efficiency of (13)C-incorporation into host cell amino acids, ... Götz, Andreas; Eylert, Eva; Eisenreich, Wolfgang and Goebel, Werner (2010): Carbon metabolism of enterobacterial human ... replicating in epithelial colorectal adenocarcinoma cells (Caco-2). To this aim, we supplied [U-(13)C(6)]glucose to Caco-2 ... The data showed that EIEC HN280 growing in the cytosol of the host cells, as well as Stm 14028 replicating in the Salmonella- ...

Cell culture models such as TC7 provide qualitative information on drug interactions involving intestinal CYP3A and Pgp. ... Caco-2p) cell monolayers using RT-PCR. The CYP3A kinetics was determined in microsomes from both cell lines. The transport, ... Results: Both TC7 and Caco-2p cells expressed mRNA for Pgp and several important CYPs. However, mRNA for CYP3A4 was detectable ... The relative affinity of CYP3A for terfenadine metabolism in the two cell lines was comparable, but the maximum reaction rate ...

Monocarboxylate Transporter-Mediated Transport of γ-Hydroxybutyric Acid in Human Intestinal Caco-2 Cells. Wing Ki Lam, Melanie ... Monocarboxylate Transporter-Mediated Transport of γ-Hydroxybutyric Acid in Human Intestinal Caco-2 Cells. Wing Ki Lam, Melanie ... Monocarboxylate Transporter-Mediated Transport of γ-Hydroxybutyric Acid in Human Intestinal Caco-2 Cells. Wing Ki Lam, Melanie ... Thank you for sharing this Drug Metabolism & Disposition article.. NOTE: We request your email address only to inform the ...

Rectum / metabolism * Signal Transduction * Vascular Endothelial Growth Factor A / blood * Vascular Endothelial Growth Factor A ... Caco-2 Cells * Carrier Proteins / blood * Carrier Proteins / metabolism* * Carrier Proteins / pharmacology ...

Darimont C, Gradoux N, de Pover A (1999). "Epidermal growth factor regulates fatty acid uptake and metabolism in Caco-2 cells ... 1998). "Differential regulation of intestinal and liver fatty acid-binding proteins in human intestinal cell line (Caco-2): ... They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long- ... They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene ...

The intestine plays a substantial role in human vitamin B6 metabolism: a Caco-2 cell model. PLoS One. 2013;8(1):e54113. ... In several cancer cell lines, B6 contributed to increased p21 gene expression via p53 activation (4). Proposed mechanisms for ... Vitamin B6 is a coenzyme in the folate metabolism pathway. Its phosphorylated metabolites are involved in amino acid metabolism ... Vitamin B(6) activates p53 and elevates p21 gene expression in cancer cells and the mouse colon. Oncol Rep. May 2014;31(5):2371 ...

Effects of Capsaicin on Energy Metabolism by Human Intestinal Epithelial Cell Line Caco-2. Junkyu Han, Hiroko Isoda. 129. Human ... Cell-to-Cell Communication for Cell Density-Controlled Bioprocesses. Wilfried Weber, Martin Fussenegger. 71. Metabolome ... Influence of Glucose and Glutamine Concentration on Metabolism of rCHO Cells. Hiroshi Matsuoka, Jun-ya Watanabe, Toshiya Takeda ... Characterizing Physiology and Metabolism of High-Density CHO Cell Perfusion Cultures Using 2D-NMR Spectroscopy. Chetan Goudar, ...

Metabolism of α-linolenic acid in humans. Prostagland, Leukotri Essent Fatty Acids 75: 161-168, 2006. ... a commonly used gastrointestinal cell line, and human embryonic kidney (HEK-293), an epithelial immortalised cell line. Caco-2 ... Cell culture. The Caco-2 and HEK-293 cell lines used in the study were obtained from Highveld Biologicals (Pty) Ltd., ... Cell numbers (percent of control) for the Caco-2 and HEK-293 cells are shown for each oil concentration. The assays were ...

Barrett, M. A., Lawrence, J. M., Hutt, A. J. & Lansley, A., 31 Dec 1997, In: European Journal of Drug Metabolism and ... Stent material surface and glucose activate mononuclear cells of contril, type 1 and type 2 diabetes subjects. Harrison, M., ... Stereoselective absorption and hydrolysis of cefuroxime axetil diastereomers using the Caco-2 cell monolayer model. ... Watt, P. W., Corbett, M. E. & Rennie, M. J., 1 Jan 1992, In: American Journal of Physiology - Endocrinology and Metabolism. 263 ...

Sun H and Pang KS (2008) Permeability, transport, and metabolism of solutes in Caco-2 cell monolayers: a theoretical study. ... 0.032 mg/106cells, n = 11). A cell volume of 3.9 μl/1 million hepatocytes (Reinoso et al., 2001) or 1.40 μl/1 million CHO cells ... Cell viability was determined from exclusion of erythrosine-B by the cell membranes. Only the cell preparations that exhibited ... Fexofenadine was tested in CHO-Oatp1a1 cells and deltorphin II and CCK8 in CHO-Oatp1b2 cells (A). In CHO cells and rat ...

Cell Culture.. Caco-2 cells were obtained from the American Type Culture Collection (Manassas, VA) and grown in Dulbeccos ... 1984) In vitro and in vivo activation of oxidative drug metabolism by flavonoids. J Pharmacol Exp Ther 229:162-170. ... LLC-PK1 cells (porcine kidney epithelial cell line) and LLC-GA5-COL300 cells (a transformant cell line derived by transfecting ... 105 for LLC-PK1 cells and 3.125 × 105 for LLC-GA5-COL300 cells. The cells were grown for 3 days, and the culture medium was ...

However, the inhibition of transporters and CYP enzymes could be tested in cell lines, for example Caco-2 cells, to have more ... To gain deeper insight on the roles of cardiac metabolism pathways and hypoxia in cell cycle activation, we evaluated the ... As brain endothelial cells are polarized in vivo, the aim of the study was to demonstrate the cell polarization of hCMEC/D3 ... Human embryonic kidney cells 293 (HEK-293) and hPREP knockout cell line created from them by using CRISPR/Cas9-silencing were ...

Cell Adhesion/drug effects, Cell Membrane/drug effects/metabolism, Cell Proliferation/drug effects, Cytoskeleton/drug effects, ... In addition, cell integrity is not damaged, since neither propidium iodide uptake nor LDH release takes place in Caco-2 cells ... We evaluated the effect of these compounds on cell morphology, cell detachment, cell proliferation, F-actin disruption and ... The human colorectal adenocarcinoma cell line Caco-2 is a widely used in vitro model of the intestinal cells. The aim of this ...

Combined cereal and pulse flavonoids show enhanced bioavailability by downregulating phase II metabolism and ABC membrane ... transporter function in Caco-2 model. - Texas A&M University (TAMU) Scholar profile, educations, publications, research, recent ... flavonoids show enhanced bioavailability by downregulating phase II metabolism and ABC membrane transporter function in Caco-2 ... Membrane permeability, phase II metabolism, and ATP binding cassette (ABC) membrane transporter expression and function were ...

CYCLOSPORINE-A METABOLISM, BILE-ACID TRANSPORTER, VILLUS-CRYPT SURFACE, ALPHA-OST-BETA, HUMAN-LIVER, CACO-2 CELLS, ... Precision-cut Intestinal Slices as In Vitro Tool for Studies on Drug Metabolism. Current drug metabolism. 2013 Jan;14(1):112- ... Precision-cut Intestinal Slices as In Vitro Tool for Studies on Drug Metabolism. Current drug metabolism, 14(1), 112-119. ... Precision-cut Intestinal Slices as In Vitro Tool for Studies on Drug Metabolism. / Groothuis, Geny M. M.; de Graaf, Inge A. M. ...

In Caco-2 cell experiments, the compound displayed modest permeability suggesting that it may show limited bioavailability via ... cell partitioning. Moreover, only a small fraction of the parent compound was excreted via feces and urine and oxidative ... it preferred to partition to red blood cells (B/P ratio: 4.91). The results of microsomal metabolic stability corroborated that ... Tiantian Wen 2 , Jia Wang 3 , Jingmiao Shi 3 , Hui Zhou 2 , Zhimeng Chen 1 , Meng Lei 1 , Yongqiang Zhu 2 ...

Sulphation of lithocholic acid in the colon-carcinoma cell line CaCo-2. Biochem J, 343 Pt 3 (Pt 3), 533-9. DOI 10.1042/0264- ... Effect of a coffee lipid (cafestol) on regulation of lipid metabolism in CaCo-2 cells. J Lipid Res, 36 (10), 2079-89. PubMed ... Effect of coffee lipids (cafestol and kahweol) on regulation of cholesterol metabolism in HepG2 cells. Arterioscler Thromb Vasc ... Plasmacytoid dendritic cells protect against atherosclerosis by tuning T-cell proliferation and activity. Circ Res, 109 (12), ...

... whereas UGT1A6 is highly expressed also in undifferentiated cells. Drug Metabolism and Disposition. 2008. doi: 10.1124/dmd. ... whereas UGT1A6 is highly expressed also in undifferentiated cells. In: Drug Metabolism and Disposition. 2008. ... In: Drug Metabolism and Disposition, 2008.. Research output: Contribution to journal › Article › Scientific › peer-review ... T1 - The expression of most UDP-glucuronosyltransferases (UGTs) is increased significantly during Caco-2 cell differentiation, ...

Cell-alteration; Cell-cultures; Cell-metabolism; Cellular-reactions; Cytotoxic-effects; Organic-compounds; Membrane-dysfunction ... Caco-2 cells were exposed to 50 µg/ml MWCNT (oxidized or pristine) for 24 h, and experiments were repeated in the presence of ... The cell monolayers also displayed tight junctions and electrical resistance. Exposure to pristine and oxidized MWCNT, with or ... Cells displayed many of the properties that characterize enterocytes, such as apical microvilli, basolateral basement membrane ...

Lactobacillus rhamnosus strain GG reduces aflatoxin B-1 transport, metabolism, and toxicity in caco-2 cells. Gratz, S., Wu, Q. ... Genoprotective Effects of Essential Oil Compounds Against Oxidative and Methylated DNA Damage in Human Colon Cancer Cells. ... Gratz, S., Wu, Q., El-Nezami, H., Juvonen, R., Mykkanen, H. & Turner, P. C., Jul 2007, In: Annals of Nutrition & Metabolism. 51 ... Gratz, S. W., Richardson, A. J., Fyfe, C., Johnstone, A. M. & Holtrop, G., Sept 2013, In: Annals of Nutrition & Metabolism. 63 ...

Cytotoxicity, genotoxicity, and cell cycle analyses were performed in HT29 cells. Cactus colonic fermentates showed higher cell ... Transport and metabolism studies showed that 1% of the total CGAs were absorbed and transported from the apical to the ... Caco-2 cells possessed low metabolic activity. In conclusion, spent coffee extracts contain large amounts of CGAs, which ... Cell viability (MTT) was not affected by spent coffee extracts (,80%) up to 1000 mu g/mL after 2 h. Both spent coffee extracts ...

Gal, A. & Csaba, G., 31 Mar 1997, In: Endocrinology and Metabolism. 4, 2, p. 115-119 5 p.. Research output: Contribution to ... Solid-State 13C NMR of Cell Walls in Wheat Bran. Ha, M-A., Jardine, W. G. & Jarvis, M. C., 1997, In: Journal of Agricultural ... Stereoselective absorption and hydrolysis of cefuroxime axetil diastereomers using the Caco-2 cell monolayer model. Barrett, M ... The voltage dependence of the transducer may modify outer hair cell motility. Russell, I. J. & Lukashkin, A. N., 1 Jan 1997, In ...

... is expressed mainly in adult hormone-sensitive cells or cells with high lipid metabolism and in proliferating fetal cells. J ... Cell culture and selection. Four human colorectal cancer cells of Caco-2, HT-29, LoVo and LS174T were used in this study. All ... Cell cycle analysis. Cells (1 × 106) were harvested, washed with PBS and fixed in 70% ethanol. The fixed cells were washed with ... Cell proliferation assay. Cells (6 × 103/200 μl/well) were seeded in 96-well plates. Viable proliferating cells were detected ...

Thiamine has a critical role in energy metabolism and cell function [255]. Low levels have been implicated in fatigue and IBD ... the Intestinal Barrier by Facilitating Tight Junction Assembly Via Activation of AMP-Activated Protein Kinase in Caco-2 Cell ... Cell 2006, 124, 837-848. [Google Scholar] [CrossRef][Green Version]. *Turnbaugh, P.J.; Ley, R.E.; Mahowald, M.A.; Magrini, V.; ... Cell 2015, 163, 1079-1094. [Google Scholar] [CrossRef]. *Ercolini, D.; Fogliano, V. Food design to feed the human gut ...

Cytotoxicity, genotoxicity, and cell cycle analyses were performed in HT29 cells. Cactus colonic fermentates showed higher cell ... Transport and metabolism studies showed that 1% of the total CGAs were absorbed and transported from the apical to the ... Caco-2 cells possessed low metabolic activity. In conclusion, spent coffee extracts contain large amounts of CGAs, which ... Cell viability (MTT) was not affected by spent coffee extracts (,80%) up to 1000 mu g/mL after 2 h. Both spent coffee extracts ...

Aluminum-induced defective mitochondrial metabolism perturbs cytoskeletal dynamics in human astrocytoma cells. J Neurosci Res. ... Fatty acids increase paracellular absorption of aluminium across Caco-2 cell monolayers. Chem Biol Interact. 2009 Oct 7. 181(2 ... Aluminum may also have a direct effect on iron metabolism: it influences absorption of iron via the intestine, it hinders ... In vivo genotoxicity assessment of aluminium oxide nanomaterials in rat peripheral blood cells using the comet assay and ...

... cells, in a dose-dependent manner. Adenine induced significant apoptosis in HT29 cells, whereas Caco-2 cells ... i,Results,/i,. Adenine significantly inhibited the viability of colon cancer cells, HT29 and Caco-2  ... signaling contributing to autophagic cell death through mTOR in both colon cancer cell lines. ,i,Conclusions,/i,. Our findings ... i,Methods,/i,. Cell viability was measured using the MTT assay. Levels of phosphorylation and protein expression were ...

... human colon cancer cell line (Caco-2) methyl capping of all free hydroxyl groups of flavones human liver S9 fraction; human ... 70) Kaldas, M. I.; Walle, U. K.; Walle, T. Resveratrol transport and metabolism by human intestinal Caco-2 cells. J. Pharm. ... TRANSPORT OF POLYPHENOLS FROM THE INTESTINAL LUMEN INTO CELLS The transport of phenolic compounds to the cell is effected by a ... cells or some clones of human colon cancer Caco-2 cell line.100 They underwent partial deacylation but no phase II conjugation ...

Anthocyanin absorption and metabolism by human intestinal Caco-2 cells-A review. International Journal of Molecular Sciences, ... Nanik Suhartatik(1), Akhmad Mustofa(2*), Ponco Mursito(3) (1) Agriculture Product Technology, Faculty of Industrial and ... 2) Agriculture Product Technology, Faculty of Industrial and Food Technology, Slamet Riyadi University, Jl. Sumpah Pemuda ... https://www.scopus.com/authid/detail.uri?authorId=56165652100&eid=2-s2.0-84900790642. Agriculture Product Technology, ...

Anthocyanin absorption and metabolism by human intestinal Caco-2 cells-A review. International Journal of Molecular Sciences, ... Nanik Suhartatik(1), Akhmad Mustofa(2*), Ponco Mursito(3) (1) Agriculture Product Technology, Faculty of Industrial and ... 2) Agriculture Product Technology, Faculty of Industrial and Food Technology, Slamet Riyadi University, Jl. Sumpah Pemuda ... https://www.scopus.com/authid/detail.uri?authorId=56165652100&eid=2-s2.0-84900790642. Agriculture Product Technology, ...