Institute of Cancer Research Repository - Squamous cell carcinoma antigen: a role in the early identification of nodal...
Squamous cell carcinoma antigen: a role in the early identification of nodal metastases in men with squamous cell carcinoma of the penis. To evaluate whether serum squamous cell carcinoma antigen (SCCAg) measurements may be of use in identifying nodal metastases in patients with SCC of the penis after treating the primary tumour. The levels of SCCAg were analysed in 11 men with penile SCC between 1994 and 2001. An elevated SCCAg level had a sensitivity of 57% (95% confidence interval, CI, 18-90%) and a specificity of 100% (CI 40-100%) for nodal metastases. Levels of SCCAg increased exponentially in patients who developed nodal metastases after treatment of the primary tumour, and were elevated before clinical or radiological evidence of nodal disease. Either the absolute level or the rate of rise of SCCAg may be a useful tool with which to follow patients after excision of the primary tumour. It may be more sensitive than computed tomography and magnetic resonanc imaging in detecting recurrence, ...http://publications.icr.ac.uk/1591/
MMP-9 Modulates Pigment Epithelium-Derived Factor Control of Angiogenesis via Proteolysis | IOVS | ARVO Journals
Purpose: : To determine the effect of matrix metalloproteinase (MMP)-mediated proteolysis on the anti-angiogenic activity of pigment epithelium-derived factor (PEDF) in vitro and determine the MMP-mediated cleavage sites within the primary sequence of PEDF. Furthermore, we have determined the effect of inhibiting MMPs on the intraocular levels of PEDF protein expressed from an adenoviral expression system in a mouse eye. Methods: : To determine the site at which PEDF is cleaved by MMP-9, purified PEDF was incubated with and without MMP-9 (1:200 mass ratio MMP to PEDF) at 37°C for 24h then with trypsin (1:40 trypsin to PEDF) overnight. The peptides were separated by reverse-phase HPLC on a C-18 column, collected, treated with 2-sulfobenzoic acid anhydride, and sequenced by MALDI-MS. The anti-angiogenic activity of MMP-treated PEDF was quantified using the mouse aortic ring bioassay and a standard invasion assay. The effect of MMP inhibition on PEDF levels was evaluated by immunoassay 1 day after ...http://iovs.arvojournals.org/article.aspx?articleid=2395125
CD104 (Integrin Beta-4) (Squamous Cell Carcinoma Antigen) Antibody - Without BSA and Azide
... , Mouse Monoclonal Antibody [Clone UM-A9 ] validated in IF, FC (AH10540-100), Abgenthttp://www.abgent.com/products/AH10540-CD104-Integrin-Beta-4-Squamous-Cell-Carcinoma-Antigen-Antibody-Without-BSA-and-Azide
Pigment epithelium-derived factor regulates microvascular permeability through adipose triglyceride lipase in sepsis | Clinical...
The integrity of the vascular barrier, which is essential to blood vessel homoeostasis, can be disrupted by a variety of soluble permeability factors during sepsis. Pigment epithelium-derived factor (PEDF), a potent endogenous anti-angiogenic molecule, is significantly increased in sepsis, but its role in endothelial dysfunction has not been defined. To assess the role of PEDF in the vasculature, we evaluated the effects of exogenous PEDF in vivo using a mouse model of cecal ligation and puncture (CLP)-induced sepsis and in vitro using human dermal microvascular endothelial cells (HDMECs). In addition, PEDF was inhibited using a PEDF-monoclonal antibody (PEDF-mAb) or recombinant lentivirus vectors targeting PEDF receptors, including adipose triglyceride lipase (ATGL) and laminin receptor (LR). Our results showed that exogenous PEDF induced vascular hyperpermeability, as measured by extravasation of Evan's Blue (EB), dextran and microspheres in the skin, blood, trachea and cremaster muscle, both ...http://www.clinsci.org/content/129/1/49
Is Pigment Epithelium-Derived Factor a Real Anti-Angiogenic Factor? | IOVS | ARVO Journals
Purpose: : It has been reported that Pigment Epithelium-Derived Factor (PEDF) is a potent anti-angiogenic factor, whose concentration in the vitreous is reported to be around 1500 ng/ml. However, the precise mechanisms are not clarified to date. Therefore, we tried to examine how PEDF has suppressive effects on neovascularization by way of in vitro assays. Methods: : Bovine retinal microvascular endothelial cells (BRECs) and human umbilical vein endothelial cells (HUVECs) were used for in vitro assays. To estimate the effect of PEDF on the proliferation, migration and tube formation by endothelial cells, thymidine uptake, boyden chamber assay and tube formation assay were performed. Moreover, to investigate whether PEDF suppresses the expression of vascular endothelial growth factor (VEGF) or its receptor (VEGFR2) and inhibits VEGF-dependent phosphorylation of VEGFR2 or p44/42 MAP kinase, Northern and Western blot analyses were performed. SU5416 was used as a selective inhibitor of VEGFR2 ...http://iovs.arvojournals.org/article.aspx?articleid=2395322
PTH-329 Expression of pigment epithelium-derived factor in colorectal cancer [Abstract] -ORCA
Introduction Pigment epithelium-derived factor (PEDF) is a 50kDa, secreted glycoprotein that has been identified as a member of the serpin gene family and has been shown to exhibit neurotrophic, neuroprotective, anti-angiogenic and anti-tumourigenic effects. The aims of our study were to determine the expression profile of PEDF in a range of colorectal cell lines and its association with clinical and pathological data. Method Six human cell lines (RKO and HT115 are colonic adenocarcinoma, HRT-18 is rectal adenocarcinoma, COLO-201 is metastatic adenocarcinoma (originating from ascites), LS174T is a mucinous adenocarcinoma, and CCD-33C0 is a normal colorectal fibroblast cell line) were analysed using polymerase chain reaction (PCR) and quantitative transcript analysis (qPCR). Primary colorectal cancer tissue was collected at operation and analysed using qPCR. Results PEDF transcript was positive in RKO, HRT-18, LS174T and CCD-33C0 cell lines but negative in HT115 and COLO-201. On qPCR, PEDF ...http://orca-mwe.cf.ac.uk/80593/
The effects of Pigment Epithelium-Derived Factor on Epithelial-to-mesenchymal transition of the retinal pigment epithelium...
Purpose : Proliferative vitreoretinopathy (PVR) may lead to irreversible vision loss. Epithelial-to-mesenchymal transition (EMT) of retinal pigment epithelium (RPE) is a critical event in the pathogenesis of PVR. Downregulation of Pigment Epithelium-Derived Factor (PEDF) is demonstrated to be associated with increased EMT in some cancers. The aim of the current study was to investigate the effects of PEDF on EMT induced by TGF-β in RPE cells. Methods : Sub-confluent human RPE cells were cultured in DMEM and pretreated with PEDF (10, 50,100, 200ng/ml) for 24hs and then stimulated with recombinant TGF-β2 (10ng/ml) for additional 24, 48 and 72 hrs with or without PEDF. The expressions of α-smooth muscle actin (α-SMA, an EMT marker) and fibronectin (FN) were examined using immunofluorescent staining, qRT-PCR and Western blotting respectively. The sub-confluent human RPE cells were cultured in DMEM and pretreated with PEDF (10, 50,100ng/ml) for 24hs and then stimulated with 20ng/ml PDGF-BB. RPE ...http://iovs.arvojournals.org/article.aspx?articleid=2642688
Pigment Epithelium-Derived Factor of Glial Origin enhances Survival of Retinal Ganglion Cells | IOVS | ARVO Journals
Purpose: Retinal neurodegeneration secondary to diabetic and hypertensive retinopathy and glaucoma can lead to irreversible loss of vision and is today among the leading causes of blindness worldwide. Our purpose was to show that pigment epithelium-derived factor (PEDF), secreted by the retinal pigment epithelium and Müller glial cells (MGC), exerts neuroprotective and neurotrophic actions towards retinal ganglion cells (RGC) in a cell culture model.. Methods: Homotypic and co-culture experiments using immunoisolated primary RGC and cultured Müller glial cells were conducted for 24 hours under normoxic (95% air; 5% CO2) and hypoxic conditions (0% O2; 5% CO2; 95% N2). PEDF was substituted to homotypic RGC cultures or depleted from RGC/MGC co-cultures by adding appropriate antibodies or by pretreatment of MGC with appropriate small interfering RNA (siRNA) directed against PEDF encoding RNA.. Results: After 24 hours of normoxic treatment RGC survival rate was 54.04±0.03% in homotypic and ...http://iovs.arvojournals.org/article.aspx?articleid=2335910
PEDF - Wikipedia
Pigment epithelium-derived factor (PEDF) also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functions. Found in vertebrates, this 50 kDa protein is being researched as a therapeutic candidate for treatment of such conditions as choroidal neovascularization, heart disease, and cancer. In humans, pigment epithelium-derived factor is encoded by the SERPINF1 gene. Pigment epithelium-derived factor (PEDF) was originally discovered by Joyce Tombran-Tink and Lincoln Johnson in the late 1980s. This group was studying human retinal cell development by identifying secreted factors produced by the retinal pigmented epithelium (RPE), a layer of cells that supports the retina. Upon noticing RPE produced a factor that promoted the differentiation of primitive retinal cells into cells of a neuronal phenotype, they set out to determine the identity of the factor. They isolated proteins unique to RPE cells and tested the ...https://en.wikipedia.org/wiki/PEDF
Bayesian phylogeny analysis of vertebrate serpins illustrates evolutionary conservation of the intron and indels based six...
The serpin superfamily is characterized by proteins that fold into a conserved tertiary structure and exploits a sophisticated and irreversible suicide-mechanism of inhibition. Vertebrate serpins are classified into six groups (V1-V6), based on three independent biological features-genomic organization, diagnostic amino acid sites and rare indels. However, this classification system was based on the limited number of mammalian genomes available. In this study, several non-mammalian genomes are used to validate this classification system using the powerful Bayesian phylogenetic method. This method supports the intron and indel based vertebrate classification and proves that serpins have been maintained from lampreys to humans for about 500 MY. Lampreys have fewer than 10 serpins, which expand into 36 serpins in humans. The two expanding groups V1 and V2 have SERPINB1/SERPINB6 and SERPINA8/SERPIND1 as the ancestral serpins, ...https://peerj.com/articles/1026/
IJMS | Free Full-Text | Expression and Effects of High-Mobility Group Box 1 in Cervical Cancer | HTML
We investigated the significance of high- mobility group box1 (HMGB1) and T-cell-mediated immunity and prognostic value in cervical cancer. HMGB1, forkhead/winged helix transcription factor p3 (Foxp3), IL-2, and IL-10 protein expression was analyzed in 100 cervical tissue samples including cervical cancer, cervical intraepithelial neoplasia (CIN), and healthy control samples using immunohistochemistry. Serum squamous cell carcinoma antigen (SCC-Ag) was immunoradiometrically measured in 32 serum samples from 37 cases of squamous cervical cancer. HMGB1 and SCC-Ag were then correlated to clinicopathological characteristics. HMGB1 expression tends to increase as cervical cancer progresses and it was found to be significantly correlated to FIGO stage and lymph node metastasis. These findings suggest that HMGB1 may be a useful prognostic indicator of cervical carcinoma. In addition, there were significant positive relationships between HMGB1 and FOXP3 or IL-10 expression (both p | 0.05). In contrast, HMGB1http://www.mdpi.com/1422-0067/15/5/8699/htm
The Relationship of the Expression of Vascular Endothelial Growth Factor (VEGF) and Pigment Epithelium-Derived Factor (PEDF) in...
Purpose: : To determine the changes of the expression of VEGF and PEDF from human retinal pigment epithelium (RPE) in diabetic condition as in vitro and to measure the vitreous levels of VEGF and PEDF and the expression of VEGF and PEDF in fibrovascular membranes of proliferative diabetic retinopathy (PDR) patients as in vivo. Methods: : Cultured human RPE cells were stimulated with hypoxia (CoCl2) and high glucose concentration (4500 mg/L). RT-PCR was used to examine the expression of VEGF and PEDF mRNA. Western blot was used to detect the levels of VEGF and PEDF proteins. Vitreous samples were collected from 60 eyes of 60 patients with macular hole (control group, n=10), inactive PDR (n=25), and active PDR (n=25). The levels of VEGF and PEDF were measured by enzyme-linked immunosorbent assay (ELISA). Fibrovascular proliferative membranes were collected from 6 eyes with inactive PDR (n=3) and active PDR (n=3). The expression of VEGF and PEDF in the membranes was determined by ...http://iovs.arvojournals.org/article.aspx?articleid=2391059
VEGFR-1 Is Differentially Regulated by Pigment Epithelium-Derived Factor (PEDF) via Presenilin in RPE and Retinal Endothelial...
Purpose: : To compare the regulation of VEGFR-1 expression and cleavage in RPE and retinal endothelial cells (RECs) and to determine if this is linked with presenilin-1. Methods: : Human and bovine RPE and RECs were grown to confluence and exposed to a) VEGF (100ng/ml) or PEDF (100ng/ml) alone for 24 hr, or b) VEGF for 1hr followed by PEDF for 24 hr, or c) PEDF for 1hr followed by VEGF for 24 hr. The expression and localisation of VEGFR-1 and presenilin was assessed by immunocytochemistry and confocal microscopy. Western blot analysis was undertaken on extracted protein using antibodies against the C-terminus and the N-terminus of VEGFR-1 as well as presenilin-1. Results: : Addition of PEDF to VEGF-stimulated RECs resulting in the appearance of a low molecular weight VEGFR-1 C-terminal fragment that was not present in cells treated with either VEGF or PEDF alone. By contrast, a number of different molecular weight C-terminal VEGFR-1 fragments were observed in untreated RPE cells. These were ...http://iovs.arvojournals.org/article.aspx?articleid=2395118
Squamous cell carcinoma antigen recognized by T-cells 3
The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing. [provided by RefSeq, Jul 2008 ...https://pharos.nih.gov/idg/targets/Q15020
ActoFactor™ Recombinant Human Maspin | Creative Bioarray
Maspin (mammary serine protease inhibitor) is a non-inhibitory serpin that is expressed predominantly in normal mammary epithelial cells but at significantly reduced levels or absent in most breast carcinomas. It has the ability to block the growth, invasiveness, and metastatic potential of breast and lung tumors. This anti-tumor activity is achieved, in part, by the ability of Maspin to inhibit angiogenesis and to preferentially promote apoptosis of tumor cells. Recombinant human Maspin is a 42.2 kDa non-glycosylated protein containing 375 amino acid residues ...https://www.creative-bioarray.com/ActoFactor-Recombinant-Human-Maspin-CSC-CTK0449-item-3775.htm
Hitchhiker's Guide to the Galaxy on Serpin
The majority of serpin diseases are due to protein aggregation and are termed "serpinopathies". Serpins are vulnerable to disease-causing mutations that promote formation of misfolded polymers due to their inherently unstable structures. Well-characterised serpinopathies include α1-antitrypsin deficiency (alpha-1), which may cause familial emphysema and sometimes liver cirrhosis, certain familial forms of thrombosis related to antithrombin deficiency, types 1 and 2 hereditary angioedema (HAE) related to deficiency of C1-inhibitor, and familial encephalopathy with neuroserpin inclusion bodies (FENIB; a rare type of dementia caused by neuroserpin polymerisation).. Each monomer of the serpin aggregate exists in the inactive, relaxed conformation (with the RCL inserted into the A-sheet). The polymers are therefore hyperstable to temperature and unable to inhibit proteases. Serpinopathies therefore cause pathologies similarly to other proteopathies (e.g. prion diseases) via ...http://hitchhikersgui.de/Serpin
Most recent papers with the keyword SERPINF1 | Read by QxMD
Pigment epithelium-derived factor (PEDF) encoded by serpinf1 is a potent antiangiogenic factor found in a wide variety of fetal and adult tissues. Several reports have shown that lack of PEDF leads to osteogenesis imperfecta (OI) type VI whose hallmark is a defect in mineralization that leads to excessive osteoid build up that fails to mineralize. Because PEDF is antiangiogenic factor it would pose serious consequences on bone development and healing of fractures. To understand possible mechanisms by which PEDF plays a role in bone development and regulation of matrix mineralization, we determined the effects of exogenous PEDF on vascular endothelial growth factor (VEGF) expression by human mesenchymal stem cells (hMSCs) and mechanisms of its regulation by PEDF ...https://www.readbyqxmd.com/keyword/36143
Serpin B3/SCCA1 Research Products: Novus Biologicals
Serpin B3/SCCA1 products available through Novus Biologicals. Browse our Serpin B3/SCCA1 product catalog backed by our Guarantee+.https://www.novusbio.com/common-name/serpin-b3-scca1
Long-Range Dynamic Effects of Point Mutations Propagate through Side Chains in the Serine Protease Inhibitor Eglin c †
Recombinant Human PEDF protein (ab86705) References
References for Abcam's Recombinant Human PEDF protein (ab86705). Please let us know if you have used this product in your publicationhttp://www.abcam.com/recombinant-human-pedf-protein-ab86705-references.html
GMS | 21st Annual Meeting of the German Retina Society and 8th Symposium of the International Society of Ocular Trauma (ISOT) |...
Purpose: Transplanted autologous pigment cells in suspension are well tolerated for several years in the subretinal space of AMD patients, however no significant improvement in vision is achieved. We postulate that vision improvement will require (a) that the transplanted cells be in a monolayer and (b) that the cells are genetically modified to express factors to maintain self and neural cell health and inhibit choroidal blood vessel growth. Since transfection of cells to be transplanted using viral vectors is complicated by the possible dissemination of the virus as well as severe immune reaction, we have explored transfection of pigment epithelium-derived factor (PEDF) in pigment cells using non-viral protocols. Methods: Three recombinant plasmids p-N-PEDF-GFP, p-N-PEDF IV, and p-L-GFP-PEDF IV were transfected into human RPE cells by electroporation. Results: Using electroporation RPE cells can be transfected in vitro with an efficiency of up to 80%. Sequencing of the different PEDF plasmids ...http://www.egms.de/static/en/meetings/rg2008/08rg125.shtml
Crystal structure of protein Z-dependent inhibitor complex shows how protein Z functions as a cofactor in the membrane...
Protein Z (PZ) binds to PZ-dependent inhibitor (ZPI) and accelerates the inhibition of the coagulation protease, activated factor X (FXa), in the presence of phospholipids and Ca2+. A 2.3A resolution crystal structure of PZ complexed with ZPI shows that ZPI is a typical serine protease inhibitor and that PZ has a serine protease fold with distorted oxyanion hole and S1 pocket. The 2 molecules bind with fully complementary surfaces spanning over 2400A(2) and involving extensive ionic and hydrophobic interactions. ZPI has an unusual shutter region with a negatively charged residue buried within the hydrophobic core of the molecule. This unique Asp(213) is critical in maintaining the balanced metastability required for optimal protease inhibition, especially when PZ is bound, with its replacement with Asn resulting in increased thermal stability, but decreased efficiency of protease inhibition. The structure of ZPI shows negatively and positively charged surfaces on top of the molecule, in keeping withhttps://www.semanticscholar.org/paper/Crystal-structure-of-protein-Z-dependent-inhibitor-Wei-Yan/346ff16c6186e279195fae64d485b9e2a23f6c70
E-mail a Wiley Online Library Link
Yukun Cao, Ting Yang, Chunhu Gu and Dinghua Yi Pigment epithelium-derived factor delays cellular senescence of human mesenchymal stem cells in vitro by reducing oxidative stress Cell Biology International 37. Version of Record online: 29 JAN 2013 , DOI: 10.1002/cbin.10041. Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf. Required = Required Field. ...http://onlinelibrary.wiley.com/emailArticleLink?doi=10.1002/cbin.10041&issueDoi=10.1002/cbin.v37.4
Cellular RNA Interference Mechanisms (Progress in Molecular by Dirk Grimm - Welcome to West E-books
Serpin Structure and Evolution: 501 (Methods in Enzymology). Serpins are a gaggle of proteins with comparable buildings that have been first pointed out as a suite of proteins capable of inhibit proteases. This quantity within the tools in Enzymology sequence comprehensively covers this subject. With a global board of authors, this quantity covers matters such as Crystallography of serpins and serpin complexes, Serpins as hormone transporters, and creation of serpins utilizing telephone loose structures. ...http://westdown.org.uk/pdf/cellular-rna-interference-mechanisms-progress-in-molecular-biology-and
Proteinase Inhibitors chọn lọc - TaiLieu.VN
The serpins are of general protein chemical interest due to their ability to undergo a large conformational change consisting of the insertion of the reactive centre loop (RCL) as strand 4 of the central b sheet A. To make space for the incoming RCL, the 'shutter region' opens by the b strands 3A and 5A sliding apart over the underlying a helix B. Loop insertion occurs during the formation of complexes of serpins with their target serine proteinases and during latency transition. This type of loop insertion is unique to plasminogen activator inhibitor-1 (PAI-1). ...http://tailieu.vn/tag/proteinase-inhibitors.html