RAPD - Wikipedia
Cleaved amplified polymorphic sequence Genetic marker "rDNA: Random Amplification of Polymorphic DNA (RAPD)". www.rvc.ac.uk. ... Its resolving power is much lower than targeted, species-specific DNA comparison methods, such as short tandem repeats. In ... Nearly all RAPD markers are dominant, i.e. it is not possible to distinguish whether a DNA segment is amplified from a locus ... DNA fragments from PCR amplification of random segments of genomic DNA with single primer of arbitrary nucleotide sequence and ...https://en.wikipedia.org/wiki/RAPD
DNA flow cytometry enabled ploidy and the genome size of Musa genotypes to be determined and was also used to monitor ... Screening techniques were developed to obtain disease (Fusarium wilt, black Sigatoka) and nematode resistant plants, while ... cytochimera dissociation and chromosomal stability, while, molecular marker technology was integrated into Musa breeding ... entitled Cellular biology and biotechnology including mutation techniques for creation of new useful banana genotypes . In 1996 ...http://www.fao.org/docrep/007/ae216e/ae216e07.htm
DNA profiling - Wikipedia
In a routine DNA paternity test, the markers used are short tandem repeats (STRs), short pieces of DNA that occur in highly ... This technique was also faster than RFLP analysis and used PCR to amplify DNA samples. It relied on variable number tandem ... Tautz D. (1989). "Hyper-variability of simple sequences as a general source for polymorphic DNA markers". Nucleic Acids ... Forensic scientists amplify the HV1 and HV2 regions of the mtDNA, and then sequence each region and compare single-nucleotide ...https://en.wikipedia.org/wiki/DNA_profiling
SNP Markers and Their Impact on Plant Breeding
PCR-based markers that include random amplification of polymorphic DNA (RAPD) , amplified fragment length polymorphism (AFLP ... a single locus with major effect on oleic acid was mapped to a 4 cM genetic interval by using SNP haplotypes at 8,590 loci. The ... P. Vos, R. Hogers, M. Bleeker et al., "AFLP: a new technique for DNA fingerprinting," Nucleic Acids Research, vol. 23, no. 21, ... they are anonymous and the level of their reproducibility is very low due to the non-specific binding of short, random primers ...https://www.hindawi.com/journals/ijpg/2012/728398/
Marker-assisted selection - Wikipedia
... random amplification of polymorphic DNA (RAPD), amplified fragment length polymorphism (AFlP), and single nucleotide ... DNA-based- Including microsatellites (also known as short tandem repeats, STRs, or simple sequence repeats, SSRs), restriction ... Generally the first step is to map the gene or quantitative trait locus (QTL) of interest first by using different techniques ... whereas markers (a DNA sequence or the morphological or biochemical markers produced due to that DNA) are genetically linked to ...https://en.wikipedia.org/wiki/Marker-assisted_selection
The androgen receptor and DXS15-134 markers show a high rate of discordance for germline X chromosome inactivation | Journal of...
... using a unique short tandem repeat (STR) site at DXS15-134.7 This marker has been localised distal to the AR locus at Xq28 and ... between either the varying digest or amplifying identical aliquots of DNA from a single digest. ... Generally, only two alleles were amplified at DXS15-134 compared to approximately 18 allelotypes for the AR locus. Random X ... Other markers and techniques were also limited by a lack of informative sites.21 22 However, since the development of the AR ...http://jmg.bmj.com/content/38/7/474
Patent US5175082 - Method of characterizing genomic dna - Google Patents
... which are capable of differentiating DNA by reference to more than one polymorphic minisatellite region or hypervariable locus ... Test samples of genomic DNA may be characterized by the use of polynucleotide probes each of which is specific for an ... informative genetic locus. Such probes may be prepared by the use of probes ... A region of human, animal or plant DNA which is comprised of tandem repeats of a short DNA sequence. All repeat units may not ...http://www.google.com.au/patents/US5175082?hl=en&output=html_text
Microsatellite - Wikipedia
The use of PCR means that microsatellite length analysis is prone to PCR limitations like any other PCR-amplified DNA locus. A ... Genetic marker Junk DNA Long interspersed repetitive element Microsatellite instability Mobile element Short interspersed ... Random microsatellite primers can be developed by cloning random segments of DNA from the focal species. These random segments ... then amplifying specific polymorphic regions of the extracted DNA by means of the polymerase chain reaction. Once these ...https://en.wikipedia.org/wiki/Microsatellite
Genetic fingerprinting - wikidoc
This technique was also faster than RFLP analysis and used PCR to amplify DNA samples. It relied on variable number tandem ... This method uses highly polymorphic regions that have short repeated sequences of DNA (the most common is 4 bases repeated, but ... One popular locus for fingerprinting was the D1S80 locus. As with all PCR based methods, highly degraded DNA or very small ... Forensic scientists amplify the HV1 and HV2 regions of the mtDNA, then sequence each region and compare single nucleotide ...http://www.wikidoc.org/index.php/Genetic_fingerprinting
High-Resolution Fine Mapping and Fluorescence in Situ Hybridization Analysis of sun, a Locus Controlling Tomato Fruit Shape,...
CT52 and TG576 were converted to codominant PCR-based markers as described previously for cleaved amplified polymorphic ... Feinberg, A. P., and B. Vogelstein, 1983 A technique for radiolabeling DNA restriction endonuclease fragments to high specific ... Genetic map of the sun locus. (A) Low-resolution map position of sun and markers on the short arm of chromosome 7 inferred from ... Third, the sun locus appears to interrupt a tandem duplication.. The size of the sun locus measured 38 kb in the wild relative ...http://www.genetics.org/content/168/4/2127
The Genetics of Autism | ELECTRONIC ARTICLES | Pediatrics
101 at this locus. Duplications can occur as interstitial tandem repeats (such that multiple copies of this locus are present ... These studies entail multiplex family screening using microsatellite markers. The DNA analysis of the affected family members ... obtained DNA samples from 99 multiplex autistic families and looked for evidence of linkage to 354 different polymorphic ... An example of single-mutation genetic heterogeneity is tuberous sclerosis caused by TSC1 on chromosome 9q in some families and ...http://pediatrics.aappublications.org/content/113/5/e472.full
Local population structure of white pine weevil (Pissodes strobi [Peck]) in interior and Sitka spruce stands in British...
An analysis using Random Amplified Polymorphic DNA (RAPD) showed three populations: Vancouver Island, Interior BC and North ... variable number of tandem repeats (VNTRs) or short tandem repeats (STRs) are tandem repeats of DNA that can be 1-6 nucleotides ... Using genetic markers and population assignment techniques to infer origin of boll weevils (Coleoptera: Curculionidae) ... Introduction A Single nucleotide polymorphism (SNP) is an alteration in a DNA sequence at a single base position. SNPs can be ...https://open.library.ubc.ca/cIRcle/collections/ubctheses/24/items/1.0071762
CSA El Bagre EPF
In regards of the short tandems repeats and to the genome per se, and our wide linkage analyses had identified a locus encoding ... and then amplifying specific polymorphic regions of the extracted DNA by means of the polymerase chain reaction (PCR). Once ... a diallelic single major locus component, a polygenic background, and a random environmental component [18-26]. Genetic linkage ... At each marker locus, an ancestral allele was defined as that allele occurring in the greatest frequency in the affected cohort ...https://studylib.net/doc/25161912/csa-el-bagre-epf
EP1256632A2 - High throughput polymorphism screening - Google Patents
Target nucleic acids are amplified using bridge amplification techniques. The detection and identification of the specific ... where the specific base incorporated by DNA polymerase is determined by the sequence at the polymorphic site. In another aspect ... polymorphic residue(s) is based on readout methods that utilize the specificity of specific enzymes for complementary DNA ... the identity of a specific base hybridized and ligated to the amplicon is determined by the sequence at the polymorphic site. ...https://patents.google.com/patent/EP1256632A2/en
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
The majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As ... without prior DNA extraction. In the model demonstrated here, an example of derived cleaved amplified polymorphic sequence ... on cell function has led to the development of diverse techniques to visualize individual RNA transcripts in single living ... DNA from armadillo passaged bacteria with a known number of repeat copies for each locus is used as a positive control. The FLA ...https://www.jove.com/visualize/abstract/19915680/analysis-of-short-tandem-repeats-by-parallel-dna-threading
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... short tandem repeats, STR) at many of these loci.5,6,7 Variable number tandem repeat (VNTR)5 analysis has been used to ... DNA from armadillo passaged bacteria with a known number of repeat copies for each locus is used as a positive control. The FLA ... Here, we present a simple method for isolating and amplifying the euchromatic segments of single polytene chromosome arms from ... Summary information includes the parameters coverage per locus, coverage per individual and number of polymorphic sites, among ...https://www.jove.com/visualize/abstract/23071651/locings-lightweight-alternative-for-assessing-suitability-next
Blood tests facts, information, pictures | Encyclopedia.com articles about Blood tests
... short tandem repeat (STR) typing, single nucleotide polymorphism (SNP) typing, mitochondrial DNA (mtDNA) analysis, human ... a technique that can amplify large amounts of specific small sequences of DNA from the human genome. It is also due to the ... An individual will have only one sequence at a particular polymorphic locus in each chromosome, but if the population bears ... The primers are DNA sequences that recognize a nonrepeated sequence in the genetic markers, and which are used by the DNA ...https://www.encyclopedia.com/medicine/divisions-diagnostics-and-procedures/medicine/blood-tests
PPT - More Molecular Genetic Technologies (Chapters 8, 9, 10) Polymerase Chain Reaction (PCR) Standard PCR method Real-time qu...
Manual dideoxy/automated fluorescent dye Pyrosequencing DNA Fingerprinting (DNA typing/profiling) Slideshow 37151 by Ava ... Excellent marker for DNA fingerprinting because:. *Polymorphic (fast-evolving). *Single locus. *Common throughout genomes of ... Microsatellites (short tandem repeats or STRs):. Heterozygote Male 5'-TAATAATAATAATAATAATAA----3'. Female 5'- ... Principles of cloning, vectors and cloning strategies -. dna cloning. dna cloning is a technique for reproducing dna fragments ...https://www.slideserve.com/Ava/polymerase-chain-reaction-pcrstandard-pcr-methodreal-time-quantitative-pcrdna-sequencing-manual-dideoxy-automated-fluore
Marker-assisted selection: an approach for precision plant breeding in the twenty-first century | Philosophical Transactions of...
DNA quantity and quality. Some marker techniques require large amounts and high quality of DNA, which may sometimes be ... This approach used flanking markers (less than 5 cM, on both sides of a target locus) for up to three QTLs in a single MAS step ... Sequence tagged site (STS), sequence characterized amplified region (SCAR) or single nucleotide polymorphism (SNP) markers that ... Ideally, the marker should be highly polymorphic in breeding material (i.e. it should discriminate between different genotypes ...http://rstb.royalsocietypublishing.org/content/363/1491/557
Simple Sequence Repeats Amplification: a Tool to Survey the Genetic Background of Olive Oils
The amplified SSR fragments and the DNA profiles from the monovarietal oil corresponded to the profiles from the leaves of the ... The most reliable SSR in providing correct allele sizing in distinguishing either single-cultivar olive oil samples or the ... To have reproducible results, PCR product purification and selection of a set of markers with a highly robust amplification ... This involved the analysis of DNA sequences using a panel of seven simple sequence repeats (SSRs) to provide genotype-specific ...http://www.ijbiotech.com/article_7124.html
A Novel Single Nucleotide Polymorphism within the 5′ Tandem Repeat Polymorphism of the Thymidylate Synthase Gene Abolishes USF...
To isolate TS-3RC DNA, PCR amplification was performed from a random population of human genomic DNA, and products were ... 3) ⇓ . These results show the presence of USF-1 and USF-2 on the chromatin at the TS locus, which includes the tandem repeats ... We have revised the previous PCR-based method for determining tandem repeat polymorphism genotype (12) into a RFLP technique ... Fragments of 241 (∗∗∗) and 213 bp (∗∗) were amplified from patient genomic DNA, corresponding to 3R and 2R genotypes, ...http://cancerres.aacrjournals.org/content/63/11/2898
MARKER MAPPING AND RESISTANCE GENE ASSOCIATIONS IN SOYBEAN - Patent application
... absence of a marker alone (as with most random amplified polymorphic DNA (RAPD) marker analysis).  Expressed Sequence ... ASH technology is based on the stable annealing of a short, single-stranded, oligonucleotide probe to a completely ... and a polymorphic chromosomal locus (e.g., an EST marker of the invention) are determined to cosegregate, the polymorphic locus ... Techniques for Marker Detection  The invention provides two EST molecular markers that are linked to known disease ...http://www.patentsencyclopedia.com/app/20110145953
Protocols and Video Articles Authored by Lihua Zhang
Mitochondrial DNA of velvet antler was amplified with random amplified polymorphic DNA (RAPD) technique and the PCR products ... Column chromatography requires for a short time to extract mitochondrial DNA of high purity with little damage of DNA molecules ... Transformants with a single copy of PDC deleted were isolated and identified by PCR and DNA sequencing, which was designated as ... Then, the first PDC deletion cassette PDC1-hisG-URA3-hisG- PDC1 (PHUHP) which contains a 1.6 kb URA3 marker gene, two copies of ...https://www.jove.com/author/Lihua_Zhang
... amplified by PCR using polymorphic markers D9S171, IFNA, and TP53, and separated by gel electrophoresis. B, cells from separate ... Analysis of clonality by amplification of short tandem repeats. Carcinomas of the female reproductive tract. Diagn Mol Pathol ... PCRs for each polymorphic microsatellite marker were repeated at least twice from the same DNA preparations and the same ... we chose microsatellite markers on these chromosomes for our clonality analysis. A number of molecular diagnostic techniques, ...http://clincancerres.aacrjournals.org/content/11/18/6512
Dienekes' Anthropology Blog: 04/2005
In the case of the former, a multiplex PCR technique will be used to type AT LEAST the 12 STR markers typed by Family Tree DNA ... Each individual was represented by 377 autosomal short tandem repeat (STR) genotypes. Malecot's genetic kinship was calculated ... Genescape of India, as Reconstructed from Polymorphic DNA Variation in the Y chromosome. Andrea Novelletto -- P1222. Y ... We address these questions using DNA sequence data from the mitochondrial (mtDNA) COIII locus, anonymous sequences from the non ...http://dienekes.blogspot.com/2005/04/