Empty Backbone) Expresses proteins in the cytoplasm as fusions to maltose-binding protein ... Mutations in maltose-binding protein that alter affinity and solubility properties. Walker IH, Hsieh PC, Riggs PD. Appl ... Any E. coli strain can be used for expression of fusion proteins; NEBExpress, BL21, or BL21(DE3) are good first choices ...https://www.addgene.org/75291/
RCSB PDB - 1MG1: HTLV-1 GP21 ECTODOMAIN/MALTOSE-BINDING PROTEIN CHIMERA Structure Summary Page
... ectodomain crystallized as a maltose-binding protein chimera reveals structural evolution of retroviral transmembrane proteins. ... PROTEIN (HTLV-1 GP21 ECTODOMAIN/MALTOSE-BINDING PROTEIN CHIMERA) A 450 Primate t-lymphotropic virus 1 Fragment: GP21 ECTODOMAIN ... HTLV-1 GP21 ECTODOMAIN/MALTOSE-BINDING PROTEIN CHIMERA. *DOI: 10.2210/pdb1mg1/pdb ... Interactions are determined by geometric criteria as described in K. Stierand, M. Rarey (2010), Drawing the PDB: Protein-ligand ...http://www.rcsb.org/pdb/explore/explore.do?structureId=1MG1
Identification of GEMC1 interacting proteinsa) WB of in | Open-i
WB of indicated proteins on pull-downs performed with amylose resin that was untreated (Mock), pre-bound to MBP ( ... A pull-down with recombinant xGEMC1 fused to Maltose Binding Protein (MBP) and incubated in egg extract co-precipitated Cdc45, ... A pull-down with recombinant xGEMC1 fused to Maltose Binding Protein (MBP) and incubated in egg extract co-precipitated Cdc45, ... c) Chromatin binding of the indicated proteins at different times after addition of sperm nuclei (3000 n/μl) to egg extracts ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC2875115_ukmss-28864-f0003&req=4
Anti-Maltose Binding Protein antibody (ab21144) | Abcam
Rabbit polyclonal Maltose Binding Protein antibody validated for WB, ELISA. Referenced in 1 publication and 3 independent ... Anti-Maltose Binding Protein antibody. See all Maltose Binding Protein primary antibodies. ... Anti-Maltose Binding Protein antibody (ab21144) at 1/5000 dilution. Secondary. Anti-Rabbit antibody (Sigma A0545) at 1/16000 ... Western blot - Anti-Maltose Binding Protein antibody (ab21144)This image is courtesy of an anonymous Abreview ...https://www.abcam.com/maltose-binding-protein-antibody-ab21144.html
RCSB PDB - 1MH3: maltose binding-a1 homeodomain protein chimera, crystal form I Methods Report Page
Insights into binding cooperativity of MATa1/MATalpha2 from the crystal structure of a MATa1 homeodomain-maltose binding ...http://www.rcsb.org/pdb/explore/materialsAndMethods.do?structureId=1MH3
Maltose-binding protein - Wikipedia
N-Terminal Fusion of Target Protein to Maltose-Binding Protein at Michigan Technological University maltose-binding protein at ... Maltose-binding protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the ... The fusion protein binds to amylose columns while all other proteins flow through. The MBP-protein fusion can be purified by ... Ferenci, T; Randall, LL (1979). "Precursor maltose-binding protein is active in binding substrate". J Biol Chem. 254 (20): 9979 ...https://en.wikipedia.org/wiki/Maltose-binding_protein
RCSB PDB - Protein Feature View - Maltose ABC transporter, periplasmic maltose-binding protein - Q9S5Y1 (Q9S5Y1...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... Protein Feature View of PDB entries mapped to a UniProtKB sequence * Number of PDB entries for G4FGM4: no matching PDB entries ... Protein disorder predictions are based on JRONN (Troshin, P. and Barton, G. J. unpublished), a Java implementation of RONN * ... This protein in other organisms (by gene name): G4FGM4 - Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) ...http://www.rcsb.org/pdb/protein/G4FGM4
RCSB PDB - 1MPB: MALTODEXTRIN-BINDING PROTEIN (MALTOSE-BINDING PROTEIN) MUTANT, WITH ARGININE REPLACING TRYPTOPHAN AT POSITION...
MALTOSE-BINDING PROTEIN) MUTANT, WITH ARGININE REPLACING TRYPTOPHAN AT POSITION 230 (TRP-230-ARG) ... MALTODEXTRIN-BINDING PROTEIN (MALTOSE-BINDING PROTEIN) MUTANT, WITH ARGININE REPLACING TRYPTOPHAN AT POSITION 230 (TRP-230-ARG) ... A mutant of the periplasmic maltose-binding protein (MBP) with altered transport properties was studied. A change of residue ... A mutant of the periplasmic maltose-binding protein (MBP) with altered transport properties was studied. A change of residue ...https://www.rcsb.org/structure/1MPB
Escherichia coli Maltose-Binding Protein Induces M1 Polarity of RAW264.7 Macrophage Cells via a TLR2- and TLR4-Dependent Manner...
Maltose-binding protein (MBP) is a critical player of the maltose/maltodextrin transport system in Escherichia coli. Our ... Escherichia coli Maltose-Binding Protein Induces M1 Polarity of RAW264.7 Macrophage Cells via a TLR2- and TLR4-Dependent Manner ...https://www.sigmaaldrich.com/catalog/papers/25941931
Gentaur Molecular :Ray Biotech \ Mouse Anti-Maltose Binding Protein \ 128-10144-1
Mouse Anti-Maltose Binding Protein \ 128-10144-1 for more molecular products just contact us ... Mouse Anti-Maltose Binding Protein storing antibdies for longer storage periods of the Mouse Anti-Maltose Binding Protein we ... Index / Ray Biotech / Mouse Anti-Maltose Binding Protein / Product Detail : 128-10144-1 Mouse Anti-Maltose Binding Protein. ... We have also other products like : Mouse Anti-Maltose Binding Protein. Related products : Mouse Anti-Maltose Binding Protein ...http://www.antibody-antibodies.com/product_det.php?id=1782271&supplier=search&name=Mouse%20Anti_Maltose%20Binding%20Protein
Role of water in ligand binding to maltose-binding protein: insight from a new docking protocol based on the 3D-RISM-KH...
Role of water in ligand binding to maltose-binding protein: insight from a new docking protocol based on the 3D-RISM-KH ... Maltose-binding protein is a periplasmic binding protein responsible for transport of maltooligosaccarides through the ... View full text: Role of water in ligand binding to maltose-binding protein: insight from a new docking protocol based on the 3D ... Get@NRC: Role of water in ligand binding to maltose-binding protein: insight from a new docking protocol based on the 3D-RISM- ...http://nparc.nrc-cnrc.gc.ca/eng/view/object/?id=f8d89406-113d-481f-b97f-a9bc19e92c39
RCSB PDB - Obsolete - 1MBP: THE 2.3-*ANGSTROMS RESOLUTION STRUCTURE OF THE MALTOSE- OR MALTODEXTRIN-BINDING PROTEIN, A PRIMARY...
OR MALTODEXTRIN-BINDING PROTEIN, A PRIMARY RECEPTOR FOR BACTERIAL ACTIVE TRANSPORT AND CHEMOTAXIS ... THE 2.3-*ANGSTROMS RESOLUTION STRUCTURE OF THE MALTOSE- OR MALTODEXTRIN-BINDING PROTEIN, A PRIMARY RECEPTOR FOR BACTERIAL ...https://www.rcsb.org/structure/obsolete/1MBP
PDB 1mh4 structure summary ‹ Protein Data Bank in Europe (PDBe) ‹ EMBL-EBI
Insights into binding cooperativity of MATa1/MATalpha2 from the crystal structure of a MATa1 homeodomain-maltose binding ... Maltose-binding periplasmic protein, Silenced mating-type protein A1. '> Homeobox domain * Occurring in:. *Maltose-binding ... Maltose-binding periplasmic protein; Silenced mating-type protein A1. '> Homeodomain * Occurring in:. *Maltose-binding ... Maltose-binding periplasmic protein, Silenced mating-type protein A1. '> Solute-binding family 1, conserved site ...http://www.ebi.ac.uk/pdbe/entry/pdb/1mh4
Frontiers | Highly Effective Inhibition of Biofilm Formation by the First Metagenome-Derived AI-2 Quenching Enzyme |...
This was confirmed by heterologous expression of the respective QQ proteins in Klebsiella oxytoca M5a1 and monitoring biofilm ... This was confirmed by heterologous expression of the respective QQ proteins in Klebsiella oxytoca M5a1 and monitoring biofilm ... Overall, we propose that the identified novel QQ-2 protein efficiently inhibits AI-2 modulated biofilm formation by modifying ... Evaluating the ability of the respective purified QQ-proteins to prevent biofilm formation of several model systems ...https://www.frontiersin.org/articles/10.3389/fmicb.2016.01098/full
Patent US7282572 - Method and device for improving protein stability and solubility - Google Patents
Protein expression is driven by a strong bacterial promoter (TAC). The expression is induced by the addition of 1 mM IPTG that ... are cloned into the multiple cloning site of the Vector System just downstream of the p26 or alpha crystallin type protein and ... A method for expressing proteins as a fusion chimera with a domain of p26 or alpha crystallin type proteins to improve the ... protein stability and solubility when over expressed in bacteria such as E. coli is provided. Genes of interest ...http://www.google.com/patents/US7282572
Integration of ATP, cAMP, and Ca2+ Signals in Insulin Granule Exocytosis | Diabetes
... maltose-binding protein; NBF, nucleotide-binding fold; PKA, protein kinase A; SNAP, soluble N-ethylmaleimide-sensitive factor ... was also expressed as a maltose-binding protein (MBP)-fused protein in BL21. These fusion proteins were purified by affinity ... Binding of SUR1, cAMP-GEFII, and Piccolo (A) and binding of Piccolo and VDCC (B). A: FLAG-cAMP-GEFII expressed in COS-1 cells ... We previously identified the cAMP-binding protein cAMP-GEFII by a yeast two-hybrid screen of MIN6 cDNA with a partial SUR1 ( ...http://diabetes.diabetesjournals.org/content/53/suppl_3/S59.full
... to produce recombinant fusion proteins with the maltose binding protein (MBP) at the NH2 terminus of the viral protein. After ... maltose binding protein; PBMC, peripheral blood mononuclear cells; TCM, central memory T-cell; TEM, effector memory T-cell; TT ... and VP4 structural proteins and the P2C nonstructural protein. cDNAs encoding these CVB4 proteins were cloned, expressed, and ... As a control in the assays, the MBP protein alone was also purified in the same way as the MBP viral fusion proteins. ...http://diabetes.diabetesjournals.org/content/51/6/1745
Vasostatin, a Calreticulin Fragment, Inhibits Angiogenesis and Suppresses Tumor Growth | JEM
IFN-γ inducible protein-10. MBP. maltose-binding protein. Mig. monokine induced by IFN-γ. VEGF. vascular endothelial growth ... The expression of the NH2-terminal domain of human calreticulin fused to maltose-binding protein (MBP) in Escherichia coli was ... Protein Sequencing.. Protein spots were excised from the Coomassie-stained gel and destained. The proteins were digested with ... 1994) Calreticulin: not just another calcium-binding protein. Mol Cell Biochem 135:71-78, pmid:7816058.. ...http://jem.rupress.org/content/188/12/2349
REG-γ associates with and modulates the abundance of nuclear activation-induced deaminase | JEM
A fusion protein between AID and maltose-binding protein (MBP; MBP-AID) was coexpressed in E. coli with His-tagged REG-γ. ... Maltose binding proteins were purified from the clarified lysates by binding for 1 h at 4°C on to amylose resin (NEB) and ... The Protein G-tagged proteins were purified by binding to IgG-Sepharose. After incubation with TEV protease, the eluted FLAG3- ... This MBP-AID truncation protein also binds REG-γ, indicating that REG-γ binds residues located within the N-terminal 60% ...http://jem.rupress.org/content/208/12/2385
A single domain antibody fragment that recognizes the adaptor ASC defines the role of ASC domains in inflammasome assembly | JEM
maltose-binding protein. PA. protective antigen. PMA. phorbol-12-myristate-13-acetate. PYD. Pyrin domain. WR. Western Reserve. ... We immunized an alpaca with purified ASC, produced as a fusion with maltose-binding protein (MBP). After five booster ... and lysates were bound to the VHH beads in the cold. After extensive washes, bound proteins were eluted with 0.2 M glycine pH ... VHHs can induce or selectively bind distinct conformations, mask epitopes required for protein-protein interactions, or inhibit ...http://jem.rupress.org/content/early/2016/04/05/jem.20151790
Identification of a Novel Prostaglandin F2α Synthase in Trypanosoma brucei | JEM
The recombinant enzyme was produced as a fusion protein with maltose-binding protein (MBP) in the cytosolic fraction of E. coli ... 5 μg of protein was subjected to electrophoresis on a 14% SDS-polyacrylamide gel, and the protein bands were detected by silver ... Abbreviations used in this paper: AA, arachidonic acid; COX, cyclooxygenase; EIA, enzyme immunoassay; MBP, maltose-binding ... Expression of TbPGFS in E. coli and TbPGFS activity of the recombinant protein. (A) TbPGFS was expressed as a fusion protein ...http://jem.rupress.org/content/192/9/1327
Structural insights into WHAMM-mediated cytoskeletal coordination during membrane remodeling | JCB
... we developed a maltose-binding protein (MBP) tagging system for expressing high levels of soluble WHAMM fusion proteins in ... maltose-binding protein. MT. microtubule. NPF. nucleation-promoting factor. NTA. nitrilotriacetic acid. PIP. ... The domain organization of maltose-binding protein (MBP)- and polyhistidine (His)-tagged WHAMM derivatives that were used in ... To purify the MBP fusion proteins, supernatants were mixed with amylose resin (New England Biolabs, Inc.), and bound proteins ...http://jcb.rupress.org/content/199/1/111/
A mechanism for vertebrate Hedgehog signaling: recruitment to cilia and dissociation of SuFu-Gli protein complexes | JCB
maltose-binding protein. MEF. mouse embryonic fibroblast. MT. microtubule. Noc. nocodazole. Ptc. Patched. Q-PCR. quantitative ... was expressed in bacteria as a soluble fusion with maltose-binding protein (MBP). Serum from rabbits immunized with this ... The similar localization pattern of SuFu and Gli at the tips of cilia and the fact that SuFu binds Gli proteins (Pearse et al ... 5 B, top). This also indicates that SuFu is dedicated to binding Gli proteins, and in their absence, SuFu does not stably ...http://jcb.rupress.org/content/191/2/415.full
Multispan mitochondrial outer membrane protein Ugo1 follows a unique Mim1-dependent import pathway | JCB
Next, we tested whether Mim1 can bind Ugo1 precursor molecules and observed that maltose-binding protein (MBP)-Mim1 but not MBP ... maltose-binding protein. MOM. mitochondrial outer membrane. PBR. peripheral benzodiazepine receptor. PK. proteinase K. TA. tail ... were mixed with either MBP or MBP-Mim1 bound to maltose beads. The beads were washed, and then bound material was eluted with ... Two novel proteins in the mitochondrial outer membrane mediate β-barrel protein assembly. J. Cell Biol. 166:621-627. doi: ...http://jcb.rupress.org/content/194/3/397.long