In vitro Analysis for Macrophage Binding and Pro-inflammatory Responses to Candida albicans -BIO-PROTOCOL
In particular, methods involved in fluorescent labeling of live C. albicans, quantification of macrophage binding of the ... Dectin-1 is the major macrophage receptor that is known to recognize fungal Beta-glucans leading to induction of various immune ... Macrophage recognition of Candida albicans (C. albicans) is facilitated by pattern recognition receptors that interact with the ... Macrophage binding and pro-inflammatory assays Notes: * Extraction of macrophages is done in a hood within animal facility. ...http://www.bio-protocol.org/e1123
Increased susceptibility to activation and increased uptake of low density lipoprotein by cholesterol-loaded macrophages. |...
... mouse peritoneal macrophages and human monocyte-derived macrophages. In LPS-stimulated macrophages, high density lipoprotein- ... Cellular cholesterol synthesis, however, was increased by 25% in the activated macrophages. Macrophage activation, measured as ... Increased susceptibility to activation and increased uptake of low density lipoprotein by cholesterol-loaded macrophages.. J ... Macrophages (J774A.1) that were activated with either lipopolysaccharide (LPS), zymosan, or phorbol ester demonstrated a 30-35 ...http://atvb.ahajournals.org/content/12/6/745
Fractalkine Deficiency Markedly Reduces Macrophage Accumulation and Atherosclerotic Lesion Formation in CCR2−/− Mice |...
Macrophage Accumulation Is Markedly Reduced in CX3CL1−/−CCR2−/− Mice. We next asked whether failure to accumulate macrophages ... Figure 5. Macrophage infiltration of the aortic root in WT, CX3CL1−/−, CCR2−/−, and CX3CL1−/−CCR2−/− female mice, all on the ... Macrophage content in atherosclerotic lesions was similar in WT and CX3CL1−/− mice, although there was a trend toward a ... To quantify the macrophage content of the atherosclerotic lesions, we stained the aortic root sections with MOMA-2 (Serotec, ...http://circ.ahajournals.org/content/117/13/1642
Human bronchoalveolar macrophage heterogeneity demonstrated by histochemistry, surface markers and phagocytosis. - Semantic...
Such heterogeneity may reflect different stages of macrophage maturation or the existence of macrophage subpopulations with ... and two anti-monocyte/macrophage MoAb (UCHMI and RFD2); and (4) simultaneous phagocytosis of C. guillermondii and staining with ... Thus only a proportion of Ia positive AM expressed monocyte/macrophage antigens and were phagocytic. ... Human alveolar macrophages (AM) were obtained by bronchoalveolar lavage from 18 patients with a variety of conditions. For each ...https://www.semanticscholar.org/paper/Human-bronchoalveolar-macrophage-heterogeneity-dem-Gant-Hamblin/072e73426b9a70a1e605dbbd95e88d5da41696f4
A Salmonella protein causes macrophage cell death by inducing autophagy | JCB
SipB could be readily detected in macrophages infected with wild-type (A and B), but not in macrophages infected with the sipD ... 2; unpublished data), presumably because transfected macrophages detached from the dish. These macrophages exhibited obvious ... macrophages. More importantly, expression of SipB in caspase-1−/− macrophages resulted in their death. The involvement of SipB ... macrophages. We propose from these analyses that Salmonella-induced macrophage cell killing is the result of autophagy-mediated ...http://jcb.rupress.org/content/163/5/1123
Thiol Oxidative Stress Induced by Metabolic Disorders Amplifies Macrophage Chemotactic Responses and Accelerates Atherogenesis...
Macrophage Eh correlated with the macrophage content in both atherosclerotic (r2=0.346, P=0.004) and renal lesions (r2=0.480, P ... The macrophage Eh was a strong predictor of macrophage chemotaxis (r2=0.554, P,0.001). ... Macrophages are continually recruited into atherosclerotic lesions, and macrophage accumulation appears to increase in ... Our data underscore the critical role of the macrophage glutathione redox state in regulating macrophage chemotaxis and ...http://atvb.ahajournals.org/content/29/11/1779
Glass, C.K. (2001) Potential roles of the peroxisome proliferator-activated receptorγ in macrophage biology and atherosclerosis...
Potential roles of the peroxisome proliferator-activated receptorγ in macrophage biology and atherosclerosis. Journal of Endo- ... Glass, C.K. (2001) Potential roles of the peroxisome proliferator-activated receptorγ in macrophage biology and atherosclerosis ...http://www.scirp.org/reference/ReferencesPapers.aspx?ReferenceID=520967
TH2 cytokines and allergic challenge induce Ym1 expression in macrophages by a STAT6-dependent mechanism. - Oxford Neuroscience
Macrophage expression of Ym1 was highly induced in vivo by an IL-4- and STAT6-dependent mechanism during the evolution of ... Microarray analysis of the transcriptional response of mouse peritoneal macrophages to the T(H)2 cytokine interleukin-4 (IL-4) ... Molecular characterization of the Ym1 promoter in transfected epithelial and macrophage cell lines revealed the presence of ... supporting the biological relevance of the IL-4-dependent pathway characterized ex vivo in peritoneal macrophages. These ...https://www.neuroscience.ox.ac.uk/publications/20623
vOX2 glycoprotein of human herpesvirus 8 modulates human primary macrophages activity
... Cristiano Salata, Matteo Curtarello, ...https://www.growkudos.com/publications/10.1002%25252Fjcp.21722/reader
Macrophage surface antigen monoclonal antibody, clone AM-3K - (MAB1733) - Products - Abnova
Mouse monoclonal antibody raised against macrophage surface antigen. Native human alveolar macrophages. (MAB1733) - Products - ... Interspecies reactivities of anti-human macrophage monoclonal antibodies to various animal species.. Zeng L, Takeya M, Ling X, ... AM-3K, a novel monoclonal antibody specific for tissue macrophages and its application to pathological investigation.. Zeng L, ... Distribution of cells immunopositive for AM-3K, a novel monoclonal antibody recognizing human macrophages, in normal and ...http://www.abnova.com/products/products_detail.asp?catalog_id=MAB1733
Generation of anti-inflammatory macrophages for implants and regenerative medicine using self-standing release systems with a...
Macrophages are highly plastic cells that define the tissue reactions on the implanted material. Local control of macrophage ... Gelatin; Cytokine; Macrophage phenotype control; Controlled release; Wound healing. Schools/Departments:. University of ... However, in the absence of M2Ct in the medium macrophages underwent rapid pro-inflammatory re-programming upon IFNg stimulation ... Herein, we describe the development of a cytokine cocktail (M2Ct) that induces stable M2-like macrophage phenotype with ...http://eprints.nottingham.ac.uk/40797/
Genetic background affects the expansion of macrophage subsets in the lungs of Mycobacterium tuberculosis-infected hosts. -...
Our findings show a higher ratio of macrophages to M2 macrophages in the lungs of chronically infected C57BL/6 mice compared ... However, there was no difference in the functional ability of M1 and M2 macrophages for the two strains in vitro. In vivo, a ... We hypothesized that infected C57BL/6 mice would exhibit a higher frequency and function of M1 macrophages than infected BALB/c ... The contribution of our findings is that although M1 macrophage is an effector leucocyte with microbicidal machinery, its ...https://www.semanticscholar.org/paper/Genetic-background-affects-the-expansion-of-macrop-Bertolini-Souza/33d5d9d4835966ee79be57dd902912680fbcad46
Isolation of Macrophages
This procedure usually gets you ,95% pure macrophages by non-specific esterase staining. If you want inflammatory macrophages, ... Isolation of Macrophages. Jay Mone' jmone at MARAUDER.MILLERSV.EDU Tue Jun 2 06:47:20 EST 1998 *Previous message: Isolation of ... Dave: To isolate resident peritoneal macrophages, kill the mouse, and immediately inject the peritoneum with about 5 mls of pre ...http://www.bio.net/bionet/mm/cellbiol/1998-June/008849.html
killing macrophages by feeding
... brett at BORCIM.WUSTL.EDU brett at BORCIM.WUSTL.EDU Mon Apr 18 12:29:40 EST 1994 *Previous ... recently frank chisari from the scripps used carageenan to bump off the macrophages in vivo. i don't have the ref handy, but it ...http://www.bio.net/bionet/mm/immuno/1994-April/001268.html
Ironing Out Macrophages | Science Signaling
As the serum concentration of iron decreases, iron accumulates in hepatic and splenic macrophages. Kim et al. report that the ... typhimurium) exploits this system to proliferate in macrophages. S. typhimurium infection causes the host to produce the ... thus reducing both the uptake of dietary iron and the release of iron into the bloodstream by the macrophages that recycle red ... processes influenced by ERRγ but also suggests that preventing or reversing the iron accumulation that occurs in macrophages ...http://stke.sciencemag.org/content/7/323/ec112?rss=1
follicular cells and macrophages - Thyroid Disorders - MedHelp
The result was benign appearing follicular cells and macrophages. I have pain in my neck in the area of the thyroid. Difficulty ... follicular cells and macrophages mbooher1 I just had a needle biopsy of two nodules on each side of my thyroid. The result was ... follicular cells and macrophages. I just had a needle biopsy of two nodules on each side of my thyroid. The result was benign ... appearing follicular cells and macrophages. I have pain in my neck in the area of the thyroid. Difficulty swallowing and my ...https://www.medhelp.org/posts/Thyroid-Disorders/follicular-cells-and-macrophages/show/646272
Virus Macrophage stock video 473376381 | iStock
Download this Virus Macrophage video now. And search more of the web's best library of royalty-free stock video footage from ...https://www.istockphoto.com/video/virus-macrophage-gm473376381-34632932?st=4a0d471
Medical Xpress - macrophages
Macrophage. Macrophages (Greek: big eaters, from makros "large" + phagein "eat"; abbr. MΦ) are white blood cells within tissues ... Human macrophages are about 21 micrometres in diameter. Monocytes and macrophages are phagocytes, acting in both non-specific ... Genomics reveals key macrophages' involvement in systemic sclerosis. A new international study has made an important discovery ... about the key role of macrophages, a type of immune cell, in systemic sclerosis (SSc), a chronic autoimmune disease which ...https://medicalxpress.com/tags/macrophages/sort/liverank/1w/
Medical Xpress - macrophages
Macrophage. Macrophages (Greek: big eaters, from makros "large" + phagein "eat"; abbr. MΦ) are white blood cells within tissues ... Human macrophages are about 21 micrometres in diameter. Monocytes and macrophages are phagocytes, acting in both non-specific ... Mount Sinai researchers have discovered that normal immune cells called macrophages, which reside in healthy breast tissue ... injury can be aided by their ability to hitch intercellular rides to specific anti-inflammatory cells called M2 macrophages, ...https://medicalxpress.com/tags/macrophages/sort/liverank/1m/
Macrophage - New World Encyclopedia
Macrophages also play a role in Human Immunodeficiency Virus (HIV) infection. Like T cells, macrophages can be infected with ... Fixed macrophages. A majority of macrophages are stationed at strategic points where microbial invasion or accumulation of dust ... 1989, 33) call macrophages "professional phagocytic cells." Macrophages not only ingest invading microorganisms, but have an ... Steps of a macrophage ingesting a pathogen:. a. Ingestion through phagocytosis, a phagosome is formed. b. The fusion of ...http://www.newworldencyclopedia.org/entry/Macrophage
Macrophages | Arteriosclerosis, Thrombosis, and Vascular Biology
Thank you for your interest in spreading the word on Arteriosclerosis, Thrombosis, and Vascular Biology.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address. ...http://atvb.ahajournals.org/content/early/2013/05/02/ATVBAHA.113.301575
... From:. Phil Bergin We use the Dako clone Ber-MAC3 anti human CD163 which seems to be working quite well for ... macrophages. So far we have tried CD68, Ham 56. Is there a specific marker > that would only pick up the above mentioned ... identifying tissue resident macrophages. Its supposed to be good for differentiating between non-activated and activated ...http://www.histosearch.com/histonet/Oct01/Re.Macrophages.html
Macrophage fusion | JEM
Macrophage fusion: what we know. The molecular mechanisms that allow macrophages to fuse with each other and, possibly, with ... Macrophages are present in all tissues and can fuse with other macrophages to differentiate into multinucleate osteoclasts (in ... Several proteins have been identified that play a role in macrophage fusion. One of these proteins, macrophage fusion receptor ... Osteoclasts and giant cells: macrophage-macrophage fusion mechanism. Int. J. Exp. Pathol. 81:291-304. ...http://jem.rupress.org/content/202/3/337
Macrophages in AMD | HSTalks
Chi-Chao Chan on Macrophages in AMD, part of a collection of online lectures. ... there's macrophage, microglia, or resident macrophage, and lymphocytes, they're all inflammatory cells. The same type, these ... Macrophages in AMD. *Dr. Chi-Chao Chan - National Institute of Health (NIH), USA ... Chan, C. (2016, October 31). Macrophages in AMD [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks ...https://hstalks.com/t/3382/macrophages-in-amd/?biosci
Identifying functional microRNAs in macrophages with polarized phenotypes. - PubMed - NCBI
Introduction of mimics of miRNAs can alter macrophage phenotypes. A, C, and E, RNA was collected from THP-1 cells 48 h post- ... High basal miRNA expression levels in macrophages correlates with gradual accumulation of guide strands and transient ... Identifying functional microRNAs in macrophages with polarized phenotypes.. Graff JW1, Dickson AM, Clay G, McCaffrey AP, Wilson ... Macrophages respond to external stimuli with rapid changes in expression of many genes. Different combinations of external ...https://www.ncbi.nlm.nih.gov/pubmed/22549785
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