The development of macrophages from human CD34+ haematopoietic stem cells in serum-free cultures is optimized by IL-3 and SCF. ...
Adherence was used to isolate mature macrophages, and the macrophage-like phenotype was confirmed by analyses of surface ... Differentiation of macrophages from a less mature precursor, such as CD34+ haematopoietic stem cells, can further inform with ... However, for macrophage differentiation, a significant proportion of development has occurred prior to the monocyte stage; ... This study refines the means by which large numbers of macrophages are obtained under serum-free conditions from haematopoietic ...https://www.kennedy.ox.ac.uk/publications/375416
In vitro Analysis for Macrophage Binding and Pro-inflammatory Responses to Candida albicans -BIO-PROTOCOL
In particular, methods involved in fluorescent labeling of live C. albicans, quantification of macrophage binding of the ... Dectin-1 is the major macrophage receptor that is known to recognize fungal Beta-glucans leading to induction of various immune ... Macrophage recognition of Candida albicans (C. albicans) is facilitated by pattern recognition receptors that interact with the ... Macrophage binding and pro-inflammatory assays Notes: * Extraction of macrophages is done in a hood within animal facility. ...http://www.bio-protocol.org/e1123
Increased susceptibility to activation and increased uptake of low density lipoprotein by cholesterol-loaded macrophages. |...
... mouse peritoneal macrophages and human monocyte-derived macrophages. In LPS-stimulated macrophages, high density lipoprotein- ... Cellular cholesterol synthesis, however, was increased by 25% in the activated macrophages. Macrophage activation, measured as ... Increased susceptibility to activation and increased uptake of low density lipoprotein by cholesterol-loaded macrophages.. J ... Macrophages (J774A.1) that were activated with either lipopolysaccharide (LPS), zymosan, or phorbol ester demonstrated a 30-35 ...http://atvb.ahajournals.org/content/12/6/745
Human bronchoalveolar macrophage heterogeneity demonstrated by histochemistry, surface markers and phagocytosis. - Semantic...
Such heterogeneity may reflect different stages of macrophage maturation or the existence of macrophage subpopulations with ... and two anti-monocyte/macrophage MoAb (UCHMI and RFD2); and (4) simultaneous phagocytosis of C. guillermondii and staining with ... Thus only a proportion of Ia positive AM expressed monocyte/macrophage antigens and were phagocytic. ... Human alveolar macrophages (AM) were obtained by bronchoalveolar lavage from 18 patients with a variety of conditions. For each ...https://www.semanticscholar.org/paper/Human-bronchoalveolar-macrophage-heterogeneity-dem-Gant-Hamblin/072e73426b9a70a1e605dbbd95e88d5da41696f4
A Salmonella protein causes macrophage cell death by inducing autophagy | JCB
SipB could be readily detected in macrophages infected with wild-type (A and B), but not in macrophages infected with the sipD ... 2; unpublished data), presumably because transfected macrophages detached from the dish. These macrophages exhibited obvious ... macrophages. More importantly, expression of SipB in caspase-1−/− macrophages resulted in their death. The involvement of SipB ... macrophages. We propose from these analyses that Salmonella-induced macrophage cell killing is the result of autophagy-mediated ...http://jcb.rupress.org/content/163/5/1123
Thiol Oxidative Stress Induced by Metabolic Disorders Amplifies Macrophage Chemotactic Responses and Accelerates Atherogenesis...
Macrophage Eh correlated with the macrophage content in both atherosclerotic (r2=0.346, P=0.004) and renal lesions (r2=0.480, P ... The macrophage Eh was a strong predictor of macrophage chemotaxis (r2=0.554, P,0.001). ... Macrophages are continually recruited into atherosclerotic lesions, and macrophage accumulation appears to increase in ... Our data underscore the critical role of the macrophage glutathione redox state in regulating macrophage chemotaxis and ...http://atvb.ahajournals.org/content/29/11/1779
Glass, C.K. (2001) Potential roles of the peroxisome proliferator-activated receptorγ in macrophage biology and atherosclerosis...
Potential roles of the peroxisome proliferator-activated receptorγ in macrophage biology and atherosclerosis. Journal of Endo- ... Glass, C.K. (2001) Potential roles of the peroxisome proliferator-activated receptorγ in macrophage biology and atherosclerosis ...http://www.scirp.org/reference/ReferencesPapers.aspx?ReferenceID=520967
TH2 cytokines and allergic challenge induce Ym1 expression in macrophages by a STAT6-dependent mechanism. - Oxford Neuroscience
Macrophage expression of Ym1 was highly induced in vivo by an IL-4- and STAT6-dependent mechanism during the evolution of ... Microarray analysis of the transcriptional response of mouse peritoneal macrophages to the T(H)2 cytokine interleukin-4 (IL-4) ... Molecular characterization of the Ym1 promoter in transfected epithelial and macrophage cell lines revealed the presence of ... supporting the biological relevance of the IL-4-dependent pathway characterized ex vivo in peritoneal macrophages. These ...https://www.neuroscience.ox.ac.uk/publications/20623
Macrophage surface antigen monoclonal antibody, clone AM-3K - (MAB1733) - Products - Abnova
Mouse monoclonal antibody raised against macrophage surface antigen. Native human alveolar macrophages. (MAB1733) - Products - ... Interspecies reactivities of anti-human macrophage monoclonal antibodies to various animal species.. Zeng L, Takeya M, Ling X, ... AM-3K, a novel monoclonal antibody specific for tissue macrophages and its application to pathological investigation.. Zeng L, ... Distribution of cells immunopositive for AM-3K, a novel monoclonal antibody recognizing human macrophages, in normal and ...http://www.abnova.com/products/products_detail.asp?catalog_id=MAB1733
Generation of anti-inflammatory macrophages for implants and regenerative medicine using self-standing release systems with a...
Macrophages are highly plastic cells that define the tissue reactions on the implanted material. Local control of macrophage ... Gelatin; Cytokine; Macrophage phenotype control; Controlled release; Wound healing. Schools/Departments:. University of ... However, in the absence of M2Ct in the medium macrophages underwent rapid pro-inflammatory re-programming upon IFNg stimulation ... Herein, we describe the development of a cytokine cocktail (M2Ct) that induces stable M2-like macrophage phenotype with ...http://eprints.nottingham.ac.uk/40797/
Lymphokine enhances the expression and synthesis of Ia antigens on cultured mouse peritoneal macrophages. | JEM
Lymphokine enhances the expression and synthesis of Ia antigens on cultured mouse peritoneal macrophages.. R M Steinman, N ... Lymphokine enhances the expression and synthesis of Ia antigens on cultured mouse peritoneal macrophages. ... cruzi-immune spleen cells enhanced the expression of Ia antigens on proteose-peptone-elicited mouse peritoneal macrophages (M ...http://jem.rupress.org/content/152/5/1248
Genetic background affects the expansion of macrophage subsets in the lungs of Mycobacterium tuberculosis-infected hosts. -...
Our findings show a higher ratio of macrophages to M2 macrophages in the lungs of chronically infected C57BL/6 mice compared ... However, there was no difference in the functional ability of M1 and M2 macrophages for the two strains in vitro. In vivo, a ... We hypothesized that infected C57BL/6 mice would exhibit a higher frequency and function of M1 macrophages than infected BALB/c ... The contribution of our findings is that although M1 macrophage is an effector leucocyte with microbicidal machinery, its ...https://www.semanticscholar.org/paper/Genetic-background-affects-the-expansion-of-macrop-Bertolini-Souza/33d5d9d4835966ee79be57dd902912680fbcad46
Perivascular Macrophages Are the Primary Cell Type Productively Infected by Simian Immunodeficiency Virus in the Brains of...
CNS macrophages are the target of SIV infection in animals with SIVE. CNS macrophages (CD11b, green), astrocytes (GFAP, yellow ... In this study we immunophenotypically differentiate subpopulations of brain macrophages into perivascular macrophages and ... and perivascular macrophages (15)(16)(18)(19)(20)(21)(22). These brain macrophages are distinguished by their anatomic location ... perivascular macrophages) in normal brain and on macrophages within meninges and the choroid plexus. CD14 and CD45 were readily ...http://jem.rupress.org/content/193/8/905
The polysulphated polyxylan Hoe/Bay-946 inhibits HIV replication on human monocytes/macrophages - Publikationsserver der...
Due to the important role of monocytes/macrophages in the pathogenesis of AIDS, potential drugs with anti-HIV activity in ... Due to the important role of monocytes/macrophages in the pathogenesis of AIDS, potential drugs with anti-HIV activity in ... The polysulphated polyxylan Hoe/Bay-946 inhibits HIV replication on human monocytes/macrophages ... For testing the efficacy of antiviral substances, monocytes/macrophages from peripheral blood were infected, respectively, with ...https://epub.uni-regensburg.de/14253/
Tumor-associated macrophage - Wikipedia
However, it remains unclear when M1 tumor-associated macrophages are transformed to M2 tumor-associated macrophages, but tumor ... Patients with more M1 tumor-associated macrophages displayed the opposite effect. . Macrophages are the most common cells in ... growth factors and immunosuppressive cytokines that convert trophic macrophages to tumor-associated macrophages (TAM). These ... Tumor-associated macrophages are mainly of the M2 phenotype, and seem to actively promote tumor growth. The designation of M1 ...https://en.wikipedia.org/wiki/Tumor-associated_macrophage
Isolation of Macrophages
This procedure usually gets you ,95% pure macrophages by non-specific esterase staining. If you want inflammatory macrophages, ... Isolation of Macrophages. Jay Mone' jmone at MARAUDER.MILLERSV.EDU Tue Jun 2 06:47:20 EST 1998 *Previous message: Isolation of ... Dave: To isolate resident peritoneal macrophages, kill the mouse, and immediately inject the peritoneum with about 5 mls of pre ...http://www.bio.net/bionet/mm/cellbiol/1998-June/008849.html
killing macrophages by feeding
... brett at BORCIM.WUSTL.EDU brett at BORCIM.WUSTL.EDU Mon Apr 18 12:29:40 EST 1994 *Previous ... recently frank chisari from the scripps used carageenan to bump off the macrophages in vivo. i don't have the ref handy, but it ...http://www.bio.net/bionet/mm/immuno/1994-April/001268.html
Ironing Out Macrophages | Science Signaling
As the serum concentration of iron decreases, iron accumulates in hepatic and splenic macrophages. Kim et al. report that the ... typhimurium) exploits this system to proliferate in macrophages. S. typhimurium infection causes the host to produce the ... thus reducing both the uptake of dietary iron and the release of iron into the bloodstream by the macrophages that recycle red ... processes influenced by ERRγ but also suggests that preventing or reversing the iron accumulation that occurs in macrophages ...http://stke.sciencemag.org/content/7/323/ec112?rss=1
follicular cells and macrophages - Thyroid Disorders - MedHelp
The result was benign appearing follicular cells and macrophages. I have pain in my neck in the area of the thyroid. Difficulty ... follicular cells and macrophages mbooher1 I just had a needle biopsy of two nodules on each side of my thyroid. The result was ... follicular cells and macrophages. I just had a needle biopsy of two nodules on each side of my thyroid. The result was benign ... appearing follicular cells and macrophages. I have pain in my neck in the area of the thyroid. Difficulty swallowing and my ...https://www.medhelp.org/posts/Thyroid-Disorders/follicular-cells-and-macrophages/show/646272
Biochemisty of Macrophages : Ciba Foundation Symposium : 9780470718698
Biochemisty of Macrophages by Ciba Foundation Symposium, 9780470718698, available at Book Depository with free delivery ...https://www.bookdepository.com/Biochemisty-Macrophages/9780470718698
Virus Macrophage stock video 473376381 | iStock
Download this Virus Macrophage video now. And search more of the web's best library of royalty-free stock video footage from ...https://www.istockphoto.com/video/virus-macrophage-gm473376381-34632932?st=4a0d471
macrophages | Arteriosclerosis, Thrombosis, and Vascular Biology
Macrophage Transitions in Heart Valve Development and Myxomatous Valve DiseaseHighlights Alexia Hulin, Lindsey J. Anstine, ... Ly6CHi Blood Monocyte/Macrophage Drive Chronic Inflammation and Impair Wound Healing in Diabetes Mellitus Andrew Kimball, ... Macrophage-Associated Lipin-1 Enzymatic Activity Contributes to Modified Low-Density Lipoprotein-Induced Proinflammatory ... Saturated Fatty Acids Undergo Intracellular Crystallization and Activate the NLRP3 Inflammasome in MacrophagesHighlights ...http://atvb.ahajournals.org/keyword/macrophages
Medical Xpress - macrophages
Macrophage. Macrophages (Greek: big eaters, from makros "large" + phagein "eat"; abbr. MΦ) are white blood cells within tissues ... Human macrophages are about 21 micrometres in diameter. Monocytes and macrophages are phagocytes, acting in both non-specific ... Genomics reveals key macrophages' involvement in systemic sclerosis. A new international study has made an important discovery ... about the key role of macrophages, a type of immune cell, in systemic sclerosis (SSc), a chronic autoimmune disease which ...https://medicalxpress.com/tags/macrophages/sort/liverank/1w/
Medical Xpress - macrophages
Macrophage. Macrophages (Greek: big eaters, from makros "large" + phagein "eat"; abbr. MΦ) are white blood cells within tissues ... Human macrophages are about 21 micrometres in diameter. Monocytes and macrophages are phagocytes, acting in both non-specific ... Mount Sinai researchers have discovered that normal immune cells called macrophages, which reside in healthy breast tissue ... injury can be aided by their ability to hitch intercellular rides to specific anti-inflammatory cells called M2 macrophages, ...https://medicalxpress.com/tags/macrophages/sort/liverank/1m/
Macrophage - New World Encyclopedia
Macrophages also play a role in Human Immunodeficiency Virus (HIV) infection. Like T cells, macrophages can be infected with ... Fixed macrophages. A majority of macrophages are stationed at strategic points where microbial invasion or accumulation of dust ... 1989, 33) call macrophages "professional phagocytic cells." Macrophages not only ingest invading microorganisms, but have an ... Steps of a macrophage ingesting a pathogen:. a. Ingestion through phagocytosis, a phagosome is formed. b. The fusion of ...http://www.newworldencyclopedia.org/entry/Macrophage