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*  Scalable thioarylation of unprotected peptides and biomolecules under Ni/photoredox catalysis - Chemical Science (RSC...
... depending on thiol used) when alkyl sulfhydryl substrates are employed.40,41 Photoredox catalysis, in this regard, may offer a ... and low chemical waste. Reaction scalability (from μg to grams) has been achieved through modest reagent adjustments, and high ... from peptidic alkyl sulfhydryl groups presents a challenging, yet desirable, opportunity in synthetic cross-coupling chemistry ... in physiological or basic environments.5 This may be particularly problematic when designing clinical antibody-drug conjugates6 ...
  https://pubs.rsc.org/en/content/articlehtml/2018/sc/c7sc04292b
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... oxidation of cysteine sulfhydryl groups serves as redox sensors and signal transducers that regulate a variety of physiological ... Since low enzyme concentrations are used to make steady-state measurements, manual mixing of reagents and quenching of the ... Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry. Authors: Jennifer Parker, Ning Zhu, Mengmeng Zhu, Sixue ... 9 introduced a mouse model in which the ligation of branches of the carotid artery creates a region of low and oscillatory flow ...
  https://www.jove.com/visualize/abstract/23300845/dynamic-regulation-phenylalanine-hydroxylase-simulated-redox
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... oxidation of cysteine sulfhydryl groups serves as redox sensors and signal transducers that regulate a variety of physiological ... selective labeling of the palmitoylated cysteine using a thiol-reactive biotinylation reagent, biotin-BMCC. Purification of the ... Low-density cultures of embryonic rat hippocampal neurons have been widely used to study the localization, function, and ... The ABE assay is comprised of three biochemical steps (Figure 1): 1) irreversible blockade of unmodified cysteine thiol groups ...
  https://www.jove.com/visualize/abstract/23071722/analysis-organic-hydroperoxide-response-chromobacterium-violaceum
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... oxidation of cysteine sulfhydryl groups serves as redox sensors and signal transducers that regulate a variety of physiological ... Lower rates of reproduction constitute a negative selection factor that should reduce the number of mutant alleles in the ... Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry. Authors: Jennifer Parker, Ning Zhu, Mengmeng Zhu, Sixue ... We developed an assay that is easy to use and requires standard laboratory material and reagents. The method offers a rapid and ...
  https://www.jove.com/visualize/abstract/21304594/genome-sequence-analyses-pseudomonas-savastanoi-pv-glycinea
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... oxidation of cysteine sulfhydryl groups serves as redox sensors and signal transducers that regulate a variety of physiological ... Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry. Authors: Jennifer Parker, Ning Zhu, Mengmeng Zhu, Sixue ... In the up-down method, alternating VFHs of higher or lower forces are used on the plantar L5 dermatome to delineate flexor ... In this new protocol, we replaced both MPBH and Cys-Gly with one much more stable reagent glutamic acid hydrazide (Glu- ...
  https://www.jove.com/visualize/abstract/24587088/regulatory-specialization-xyloglucan-xg-glucuronoarabinoxylan-gax
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... oxidation of cysteine sulfhydryl groups serves as redox sensors and signal transducers that regulate a variety of physiological ... Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry. Authors: Jennifer Parker, Ning Zhu, Mengmeng Zhu, Sixue ... During MS/MS analysis, a series of reporter ions (i.e., 126-131 Da) emerge in the low mass region, providing information on ... Tandem mass tag (TMT) reagents enable concurrent identification and multiplexed quantitation of proteins in different samples ...
  https://www.jove.com/visualize/abstract/23272161/regulation-cell-wall-bound-invertase-pepper-leaves-xanthomonas
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... oxidation of cysteine sulfhydryl groups serves as redox sensors and signal transducers that regulate a variety of physiological ... Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry. Authors: Jennifer Parker, Ning Zhu, Mengmeng Zhu, Sixue ... During MS/MS analysis, a series of reporter ions (i.e., 126-131 Da) emerge in the low mass region, providing information on ... In this new protocol, we replaced both MPBH and Cys-Gly with one much more stable reagent glutamic acid hydrazide (Glu- ...
  https://www.jove.com/visualize/abstract/24058708/tomato-spotted-wilt-virus-benefits-non-vector-arthropod-tetranychus
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... oxidation of cysteine sulfhydryl groups serves as redox sensors and signal transducers that regulate a variety of physiological ... However, RhoC GTPase is expressed in metastatic cancer cells at relatively low levels. Therefore, few RhoC-specific reagents ... Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry. Authors: Jennifer Parker, Ning Zhu, Mengmeng Zhu, Sixue ... Each isobaric cysTMT tag has the same nominal parent mass and is composed of a sulfhydryl-reactive group, a MS-neutral spacer ...
  https://www.jove.com/visualize/abstract/23284973/solution-structure-oncogenic-mien1-protein-reveals-thioredoxin-like
*  Redox Regulation of Large Conductance Ca2+-activated K+ Channels in Smooth Muscle Cells | JGP
MTSEA is a thiol-specific reagent that covalently modifies free thiol groups on cysteine residues (Akabas et al., 1992; ... depending on pH and the pKa of the thiol under consideration. One possibility is that one or more sulfhydryl groups close to ... 4, concentrations as low as 5 μM diamide significantly inhibited channel activity, and nPo was only 10% of the control level at ... 6 and 8), suggesting that shifts in GSH concentration in the physiological range are likely to alter maxi-K channel activity. ...
  http://jgp.rupress.org/content/110/1/35
*  Patent US5569745 - Peptide-Chelator conjugates - Google Patents
The solution is then degassed to remove oxygen then thiol protecting groups are removed with a suitable reagent, for example ... "thiol" refers to a sulfhydryl group that may be substituted with an alkyl group to form a thioether; ... The one exception, the TKPRTKPR (SEQ ID No: 10) (tuftsin dimer) conjugate, did exhibit low accumulation in the GI tract and ... "metal chelator" refers to a molecule that forms a stable complex with a traceable metal atom under physiological conditions in ...
  http://www.google.com/patents/US5569745
*  Molecules | Free Full-Text | Radiolabeling Strategies for Tumor-Targeting Proteinaceous Drugs | HTML
In order to label proteins containing accessible thiol groups, [18F]maleimide reagents, like N-(6-[(4-[18F]fluoro-benzylidene) ... Berndt, M.; Pietzsch, J.; Wuest, F. Labeling of low-density lipoproteins using the 18F-labeled thiol-reactive reagent N-[6-(4-[ ... new reagents for radiometal chelate conjugation to antibody sulfhydryl groups undergo pH-dependent cleavage reactions. ... Furthermore these radionuclides must be capable of being attached under physiological conditions due to the heat-labile nature ...
  http://www.mdpi.com/1420-3049/19/2/2135/htm
*  IJMS | Free Full-Text | Oxidative Stress and Neurodegenerative Disorders | HTML
ROS at low or moderate concentration can play important physiological roles. However, an excessive amount of ROS under ... Under low ROS condition, a living organism cannot carry out certain physiological functions. In contrast, high levels of ROS, ... This process did not elicit cell death by itself (comparison of Non-Insult groups under higher and lower AO conditions). ... The level of NO2− can be measured with Griess reagents (see following information in text). In the cell-free assay system under ...
  http://www.mdpi.com/1422-0067/14/12/24438/htm
*  N-Ethylmaleimide - Wikipedia
NEM has been widely used to probe the functional role of thiol groups in enzymology. NEM is an irreversible inhibitor of all ... Despite repeated unsuccessful attempts to identify chemically the target thiol group, at physiological pH, NEM may form adducts ... NEM activates ouabain-insensitive Cl-dependent K efflux in low K sheep and goat red blood cells. This discovery contributed to ... N. C. Adragna, M. Di Fulvio and P.K. Lauf, J. Membrane Biology, 200:1-29, 2004 K+ Cl Cotransport: Sulfhydryl, divalent cations ...
  https://en.wikipedia.org/wiki/N-Ethylmaleimide
*  The Effect of Chromium and Boron on the Lipid Peroxidation and Antioxidant Status (In Experiment)
... the number of SH-groups of blood increases by 54%. The level of MDA was significantly lower (p,0.05), SH-groups of blood higher ... The content of sulfhydryl groups (SH) in plasma was determined by the method of Ellman [19]. To 1.5 ml of phosphate buffer pH ... Total lipids were determined using a set of reagents 'Lachema' (Czech Republic); the content of protein-by the method of Lowry ... The physiological effects of dietary boron. Crit Rev Food Sci Nutr. 2003;43:219-31. ...
  https://www.tsijournals.com/articles/the-effect-of-chromium-and-boron-on-the-lipid-peroxidation-and-antioxidant-status-in-experiment.html
*  The effects of hesperidin on idiopathic pulmonary fibrosis evaluated by histopathologial-biochemical and micro-computed...
Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem 1968; 25: ... The right and left lower zones of the lungs were separated and cylinder shapes (each 1.5 mm × 8 mm) were chosen as the Regions ... GSH is a major thiol tripeptide that plays an important role in the antioxidant mechanisms in the cell [34]. GSH is a cofactor ... Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions ...
  http://www.alliedacademies.org/articles/the-effects-of-hesperidin-on-idiopathic-pulmonary-fibrosis-evaluated-by-histopathologialbiochemical-and-microcomputed-tomography-e.html
*  S-sulfhydration as a cellular redox regulation | Bioscience Reports
Thiol-based redox regulation utilizes a characteristic feature of some redox-sensitive cysteine-SH groups in proteins, called ' ... In the next steps, GSSH and other low molecular weight hydropersulfides can transfer sulfane sulfur to other-SH groups, most of ... other sulfhydryl groups, cyanide). Not only enzymes involved in sulfur metabolism, like CSE, MST, Rhd but also proteins ... 2012) Physiological implications of hydrogen sulfide: a whiff exploration that blossomed. Physiol. Rev. 92:791-896, doi:10.1152 ...
  http://www.bioscirep.org/content/36/2/e00304
*  Direct Inhibition of Expressed Cardiac L-Type Ca2+ Channels by S-Nitrosothiol Nitric Oxide Donors | Circulation Research
... which are much less hydrophilic than are the MTS reagents. These reagents can oxidize free thiol groups and attach a neutral ... To test this idea, we first examined the effect of sulfhydryl-specific modifying reagents MTSEA, MTSET, or MTSES on IBa through ... The physiological concentrations of NO, on the other hand, are in the submicromolar range.54 Excessive amounts of NO could be ... We also tested the effect of 5 to 10 nmol/L SNAP on IBa, since it has been reported that low concentrations of NO donors ...
  http://circres.ahajournals.org/content/81/5/742
*  Patente US4426372 - Inhibition of undesired effects of platinum(II) compounds - Google Patentes
... is a pharmaceutically acceptable cation and R1 and R2 are lower aliphatic or cycloaliphatic groups) or, less preferably, ... lower alkyl)dithiocarbamate to said mammal, the lower alkyl groups of said dithiocarbamate having up to six carbon atoms. ... See Cvitkovic et al., CANCER 39:1357 (1977) and Hayes et al., CANCER 39:1372 (1977). In addition, a number of thiol reagents ... At high plasma and urine platinum concentrations, binding occurs to sulfhydryl groups of the renal tubule; maximal platinum ...
  http://www.google.es/patents/US4426372
*  Patent US5182107 - Transferrin receptor specific antibody-neuropharmaceutical or diagnostic ... - Google Patents
SPDP is a heterobifunctional crosslinking reagent that introduces thiol-reactive groups into either the monoclonal antibody or ... The thiol-reactive group reacts with a free sulfhydryl group forming a disulfide bond. ... Low affinity blood brain barrier receptor antibodies and uses therefor. US20020009444 *. Apr 25, 2001. Jan 24, 2002. Idec ... The diagnostic agent is then detected as indicative of the presence of a physiological condition for which the diagnostic agent ...
  http://www.google.com/patents/US5182107?dq=5,381,459
*  Patente US5486599 - Construction and use of synthetic constructs encoding syndecan - Google Patentes
The protein should not contain free sulfhydryl groups. In cases where both proteins to be conjugated contain free sulfhydryl ... Halogens (iodoacetyl functions) react with --SH groups at physiological pH's. Both of these reactive groups result in the ... Common thiol reactive groups include maleimides, halogens, and pyridyl disulfides. Maleimides react specifically with free ... Alternatively, a primary amine may be modified with Traut's Reagent to add a sulfhydryl (Blattler et al. (1985) Biochem 24:1517 ...
  http://www.google.es/patents/US5486599?dq=flatulence
*  CSIRO PUBLISHING | Reproduction, Fertility and Development
... a new protective reagent for SH groups. Biochemistry 3, 480-482. , Dithiothreitol, a new protective reagent for SH groups. ... Kosower, N. S., and Kosower, E. M. (1987). Thiol labeling with bromobimanes. Methods Enzymol. 143, 76-84. , Thiol labeling with ... The effect of sulfhydryl oxidation on the morphology of immature hamster epididymal spermatozoa induced to acquire motility in ... The presence of low-motile or immotile spermatozoa is one of many causes of infertility; however, this observation provides ...
  http://www.publish.csiro.au/RD/RD16225
*  Plus it
In our cortical neuronal model, exposure to high glucose increased thiol levels in the cortical neurons. Free sulfhydryl groups ... Measurement of thiol content.. Total thiol content was estimated in cell and tissue homogenates using DTNB reagent (29). ... Because lipids represent the major constituents of cell membranes, a physiological and biochemical disturbance in activities ... which would normally lower GSH levels in cortical neurons subjected to high glucose conditions. High MDA levels in cortical ...
  http://diabetes.diabetesjournals.org/content/55/12/3326
*  Tetrahydrocurcumin Potentially Attenuates Arsenic Induced Oxidative Hepatic Dysfunction in Rats | OMICS International
Recent studies have confirmed that the As+3 form of As highly toxic than As+5 and it has a higher affinity for thiol groups [2, ... Further, GSH prevent the oxidation of sulfhydryl groups [59]. In our study, GSH level decreased in the liver of As-treated rats ... Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantitation of low levels of DNA damage in individual ... The activity of the enzyme was determined by adding Ehrlich reagent and the absorbance was recorded at 555 nm after 5 min. The ...
  https://www.omicsonline.org/tetrahydrocurcumin-potentially-attenuates-arsenic-induced-oxidative-hepatic-dysfunction-in-rats-2161-0495.1000-168.php?aid=19968
*  Functionalizing Morpholino Oligos for Antisense Drug Research and Development
... and thiol-disulfide exchange reagents. The sulfhydryl group is provided in a protected form (disulfide amide, 3S, Figure 2) and ... The creation of bioconjugate reagents with spontaneously reactive or selectively reactive functional groups forms the basis for ... The high polarity of this dye makes it useful for biological and physiological applications thanks to its good water solubility ... low toxicity and exquisite specificity. However, due to their high mass (usually 6,000 to 10,000 Daltons), Morpholinos lack the ...
  http://austinpublishinggroup.com/drug-development/fulltext/drug-v3-id1021.php
*  Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation | Protocol
... sulfhydryl, and carboxyl groups of biological molecules. The current crop of cell-surface biotinylation reagents consist ... Finally, the broken disulfide bonds are reacted with iodoacetamide, consuming the labile thiol groups and preventing the bonds ... Li, Y., Cam, J., Bu, G. Low-density lipoprotein receptor family: endocytosis and signal transduction. Mol Neurobiol. 23, (1), ... 1Department of Cellular and Physiological Sciences, University of British Columbia. CITE THIS SHARE ...
  https://www.jove.com/video/55974/determining-cell-surface-expression-endocytic-rate-proteins-primary