Effects of endotoxin lipopolysaccharide administration on the somatotropic axis
The aim of this work was to study the effect of chronic activation of the immune system on the somatotropic axis. Accordingly, the changes in growth hormone (GH) secretion, circulating insulin-like growth factor-I (IGF- I) and IGF binding proteins (IGFBPs) in response to endotoxin lipopolysaccharide (LPS) administration were examined in adult male Wistar rats. Acute LPS injection (2.5, 25 or 250 μg/kg) increased serum corticosterone in a dose-dependent manner and decreased serum levels of insulin and IGF-I, serum GH concentration declined linearly as the LPS dose increased. Western ligand blot showed an increase in the 33 kDa band (corresponding to IGFBP-1 and IGFBP-2) in the rats that received the highest dose of LPS (250 μg/kg). Chronic LPS administration (250 μg/kg daily for 8 days) significantly decreased body weight, serum levels of IGF-I and pituitary GH content, whereas it increased circulating IGFBP-3 (47 kDa band), IGFBP-1 and IGFBP-2 (33 kDa band) and the 24 kDa band (which possibly ...http://abacus.universidadeuropea.es/handle/11268/6231
"Migration inhibitory action of bacterial lipopolysaccharide on progeni" by Y Ishii, M Shinoda et al.
Ishii, Y; Shinoda, M; and Shikita, M, "Migration inhibitory action of bacterial lipopolysaccharide on progenitor cells of monocyte/macrophage lineage growing in culture in the presence of colony-stimulating factor (csf-1)." (1982). Subject Strain Bibliography 1982. 3652 ...https://mouseion.jax.org/ssbb1982/3652/
Influence of an Intra-articular Lipopolysaccharide Challenge on Markers of Inflammation and Cartilage Metabolism and the...
This project established an in vivo method to identify and manipulate expression of markers of osteoarthritis (OA). Specifically, strategies that predictably induce joint inflammation to evaluate dietary methods of OA prevention in young horses have yet to be accomplished. Therefore, the 3 studies described herein were conducted to determine effectiveness of an intra-articular lipopolysaccharide (LPS) challenge on markers of inflammation and cartilage metabolism in young horses and potential of dietary glucosamine hydrochloride (HCl) to mitigate these alterations. In the first study, horses were challenged with 0.25 ng or 0.50 ng of intra-articular LPS solution or lactated ringer's solution (control). Injection of LPS increased inflammation based on synovial prostaglandin E2 (PGE2) concentrations. Carboxypeptide of type II collagen (CPII), a maker of type II collagen synthesis, also increased in a dose-dependent manner. However, clinical parameters of health were not influenced and remained ...http://oaktrust.library.tamu.edu/handle/1969.1/149284
Lipopolysaccharide-induced interleukin-8 gene expression in human granulocytes: transcriptional inhibition by interferon-γ |...
We recently showed that lipopolysaccharide (LPS) is a potent inducer of interleukin-8 (IL-8) expression in human polymorphonuclear leucocytes (PMN), at the level of both mRNA and protein, and that interferon-gamma (IFN gamma) inhibits IL-8 mRNA accumulation in stimulated PMN. To further define the molecular basis of the regulation of IL-8 gene expression in PMN, we investigated the effects of LPS and IFN gamma at both the transcriptional and post-transcriptional levels. As determined by Northern blot analysis, new protein synthesis was not required for the induction of IL-8 mRNA expression by LPS. Neither did the half-life of IL-8 mRNA in LPS-treated PMN differ from that observed in untreated cells. However, nuclear run-on analysis revealed that LPS increased the transcription of the IL-8 and IL-1 beta genes and that, in LPS-activated cells, IFN gamma markedly inhibited the rate of IL-8 gene transcription, but not that of IL-1 beta. IFN gamma did not affect IL-8 mRNA stability in LPS-treated ...http://www.biochemj.org/content/310/3/751
Activated Protein C Induces Endoplasmic Reticulum Stress and Attenuates Lipopolysaccharide-Induced Apoptosis Mediated by...
Activated Protein C Induces Endoplasmic Reticulum Stress and Attenuates Lipopolysaccharide-Induced Apoptosis Mediated by Glycogen Synthase Kinase-3β. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.http://libros.duhnnae.com/2017/jul3/149894565188-Activated-Protein-C-Induces-Endoplasmic-Reticulum-Stress-and-Attenuates-Lipopolysaccharide-Induced-Apoptosis-Mediated-by-Glycogen-Synthase-Kinase-3.php
Volume 6.06 | Feb 20 - Cord Blood News
Human CD34+ Progenitor Cells Freshly Isolated from Umbilical Cord Blood Attenuate Inflammatory Lung Injury following Lipopolysaccharide Challengehttps://www.cordbloodresearchnews.com/issue/volume-6-06-feb-20/
Aberrant Cerebellar Neurotrophin-3 Expression Induced by Lipopolysaccharide Exposure During Brain Development.
Autism is a developmental disorder affecting communication, social interaction, motor skills, and cerebellar structure and functions. Recent studies have indicated that maternal infection during brain development may be one of the risk factors for auhttp://www.biomedsearch.com/nih/Aberrant-Cerebellar-Neurotrophin-3-Expression/23319369.html
Lipopolysaccharide Injection Induces Relapses of Experimental Autoimmune Encephalomyelitis in Nontransgenic Mice via Bystander...
Thank you for your interest in spreading the word about The Journal of Immunology.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...http://www.jimmunol.org/content/175/2/959/tab-article-info
JAIRO | Tetracyclines downregulate the production of LPS-induced cytokines and chemokines in THP-1 cells via ERK, p38, and...
Tetracyclines downregulate the production of LPS-induced cytokines and chemokines in THP-1 cells via ERK, p38, and nuclear factor-κB signaling pathwaysTetracyclines downregulate the production of LPS-induced cytokines and chemokines in THP-1 cells via ERK, p38, and nuclear factor-κB signaling pathways ...http://jairo.nii.ac.jp/0039/00008301
Lipopolysaccharides | Colorado PROFILES
Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed ...https://profiles.ucdenver.edu/display/215329
"Lipopolysaccharide Receptor, Toll -Like Receptor 4, Mediated Signaling" by Sang Hoon Rhee
Genetic evidence indicating that Toll-like receptor 4 (TLR4) is the lipopolysaccharide (LPS) receptor in mice was reported. However, biochemical evidence that murine TLR4 confers LPS responsiveness has not been convincingly demonstrated. Inducible cyclooxygenase (COX-2) is selectively expressed in LPS-stimulated macrophages in part mediated through activation of NFkappaB. Thus, we determined whether murine TLR4 confers LPS responsiveness as evaluated by activation of NFkappaB and COX-2 expression. Expression of the constitutively active form (DeltaTlr4) of TLR4 in murine macrophage cell line (RAW 264.7) is sufficient to activate NFkappaB and COX-2 expression and to stimulate prostaglandin E2 synthesis. The truncated form [DeltaTlr4 (P712H)] of the missense mutant Tlr4 (P712H) found in LPS-hyporesponsive mouse strain (C3H/HeJ) inhibits LPS-induced NFkappaB activation and COX-2 expression. Inability of DeltaTlr4 (P712H) to activate NFkappaB and to induce COX-2 expression is rescued by a constitutivelyhttps://digitalcommons.lsu.edu/gradschool_disstheses/390/
Ex Vivo LPS Stimulation in Healthy and Compromised Subjects - Tabular View - ClinicalTrials.gov
Ex vivo LPS stimulation of whole blood will be a good alternative challenge to induce an inflammatory response and examine differences in the inflammatory response between healthy and compromised subjects.. The purpose of the current study is to examine the inflammatory response in a younger population (35-45 yrs old) to see whether in this population also differences in the ex vivo LPS induced cytokine response exists between healthy and compromised subjects, as is seen in elderly subjects. Overweight subjects showing a state of disturbed blood glucose control will be included as subjects with compromised health and compared to healthy lean subjects with the same age (shifting from healthy towards unhealthy, not diseased).. Hypothesis Ex vivo LPS stimulation of whole blood will induce a measurable inflammatory cytokine response in a healthy population that is different from a response of the compromised population. The investigators will include subjects aged 35-45 years that differ in health ...https://clinicaltrials.gov/ct2/show/record/NCT01656915?show_rss=Y&sel_rss=mod14
Inhibition of aldose reductase prevents lipopolysaccharide-induced inflammatory response in human lens epithelial cells. |...
Sigma-Aldrich offers abstracts and full-text articles by [Agnieszka Pladzyk, Aramati B M Reddy, Umesh C S Yadav, Ravinder Tammali, Kota V Ramana, Satish K Srivastava].https://www.sigmaaldrich.com/catalog/papers/17122129
NIOSHTIC-2 Publications Search - 20038105 - Lipopolysaccharide increases Na+, K+ -pump, but not ENaC, expression in guinea-pig...
Earlier, we found in functional experiments that lipopolysaccharide (LPS; 4 mg/kg; i.p.) hyperpolarized the epithelium by stimulating the transepithelial transport of Na+ in guinea-pig tracheal epithelium. Epithelial sodium channel (ENaC) activity and Na+,K+-pump activity were increased. In this study, we hypothesized that LPS increases the expression of ENaC and the Na+,K+-pump in the epitheliumhttps://www.cdc.gov/niosh/nioshtic-2/20038105.html
The effect of gram negative lipopolysaccaride on CD14 expression in mononuclear white blood cells
Lipopolysaccaride (LPS) is one of the main components of the Gram negative cell wall, it gets released into the blood stream when bacterial cell are destroyed or lysed by the immune system. If the levels are allowed to build up it can cause sepsis, which is potentially life threatening. CD14 is a cell surface protein which is capable of binding LPS and setting off a series of reaction to neutralise it. In this experiment three types of cells, THP-1 Monocytes, differentiated THP Macrophages and MM6 Monocytes, where incubated with 5 different strain of LPS, there were also two incubation periods. The levels of membrane bound CD14 were measured by binding with monoclonal antibodies conjugated with a fluorochrome. The samples where then read by a flow cytometer in order to determine the change in CD14 levels from no LPS to incubated samples. The soluble CD14 was measured by ELISA and the results were read by a plate reader and then analysed in comparison to known standards in order to determine the ...https://repository.cardiffmet.ac.uk/handle/10369/5167
The duration of LPS priming determines the phenotype of macrophage, but not T cell P2X7 responses - Surrey Research Insight...
Tempest-Roe, S, Tam, FW and Taylor, SRJ (2013) The duration of LPS priming determines the phenotype of macrophage, but not T cell P2X7 responses In: British Society of Immunology Annual Congress, 2013-12-02 - ?. Full text not available from this repository ...http://epubs.surrey.ac.uk/828896/
NIOSHTIC-2 Publications Search - 20030634 - Effects of lipopolysaccharide on tracheal epithelial bioelectric responses to...
We examined whether lipopolysaccharide (LPS) affects transepithelial potential difference V of guinea-pig tracheal epithelium and bioelectric responses to serosally- and mucosally-applied methacholine (MCh). V was measured in vitro using the isolated perfused trachea apparatus, which allows addition of MCh separately to the mucosal or serosal surface. Eighteen hours after LPS (4 mg/kg, Lp.) basalhttps://www.cdc.gov/niosh/nioshtic-2/20030634.html
"Role of circulating fibroblast growth factor-2 in lipopolysaccharide- " by Parnell C. Mattison, Angel A. Soler-Garcia et al.
Mattison, P. C., Soler-García, A. A., Das, J. R., Jerebtsova, M., Perazzo, S., Tang, P., & Ray, P. E. (2012). Role of circulating fibroblast growth factor-2 in lipopolysaccharide- induced acute kidney injury in mice. Pediatric Nephrology, 27(3), 469-483.. ...http://hsrc.himmelfarb.gwu.edu/smhs_peds_facpubs/385/
Bacterial lipopolysaccharides form procollag-enendotoxin complexes that trigger cartilage inflammation and degeneration:...
Introduction: We have previously reported that bacterial toxins, especially endotoxins such as lipopolysaccharides (LPS), might be important causative agents in the pathogenesis of rheumatoid arthritis (RA) in an in vitro model that simulates the potential effects of residing in damp buildings. Since numerous inflammatory processes are linked with the nuclear factor-kappa B (NF-kappa B), we investigated in detail the effects of LPS on the NF-kappa B pathway and the postulated formation of procollagen-endotoxin complexes. Methods: An in vitro model of human chondrocytes was used to investigate LPS-mediated inflammatory signaling. Results: Immunoelectron microscopy revealed that LPS physically interact with collagen type II in the extracellular matrix (ECM) and anti-collagen type II significantly reduced this interaction. BMS-345541 (a specific inhibitor of I kappa B kinase (IKK)) or wortmannin (a specific inhibitor of phosphatidylinositol 3-kinase (PI-3K)) inhibited the LPS-induced ...https://epub.ub.uni-muenchen.de/23141/index.html
Phenolic components fromRhus parviflorafruits and their inhibitory effects on lipopolysaccharide-induced nitric oxide...
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.https://www.growkudos.com/publications/10.1080%25252F14786419.2013.814050/reader
Bacterial Lipopolysaccharides | SpringerLink
The bacterial lipopolysaccharide also known as endotoxin is exhaustively covered in the present work. Central emphasis is placed upon the fine chemical structure of the lipopolysaccharide and its signhttps://link.springer.com/book/10.1007%2F978-3-7091-0733-1
LPS isolation by hot phenol/water method - Microbiology
Resently, I want to isolate LPS from E.coli by hot phenol/water method which was described by Westphal, O. & Jann, K. (1965) Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. I have an ergent need to get it. Is there anybody who can help me to get this protocol? Thank you ...http://www.protocol-online.org/biology-forums-2/posts/7187.html
Although COX-1 mRNA and protein expression are detectable in normal brain tissue (Ivanov et al., 2002; Aid et al., 2008), we are unaware of previous evidence for cerebrovascular enzyme expression under basal or endotoxin-stimulated conditions. Our findings thus seem to challenge evidence that brain COX-1 is insensitive to proinflammatory challenges (Ivanov and Romanovsky, 2004). This apparent disparity may be explained by the fact that COX-1 is normally expressed principally by parenchymal (microglial) cells that may be LPS unresponsive but that could serve to dilute regulatory effects on vascular enzyme expression. Our failure to detect LPS effects on COX-1-IR in brain homogenates is consistent with this view.. Previous evidence supporting COX-1 localization to CNS vascular or glial cells has been gathered primarily in injury or disease models. Accumulation of COX-1-expressing macrophages and upregulation by ECs and microglia has been reported after spinal injury in rats (Schwab et al., 2000a) ...http://www.jneurosci.org/content/29/41/12970
Items where Author is "Chinopoulos, Christos" - Repository of the Academy's Library
Németh, Beáta and Dóczi, Judit and Csete, Dániel and Kacsó, Gergely and Ravasz, Dóra and Kiss, Gergely and Nagy, Ádám Miklós and Horváth, Gergő and Tretter, László and Mócsai, Attila and Csépányi-Kömi, Roland and Iordanov, Iordan and Ádám, Veronika and Chinopoulos, Christos (2016) Abolition of mitochondrial substrate-level phosphorylation by itaconic acid produced by LPS-induced Irg1 expression in cells of murine macrophage lineage. FASEB JOURNAL, 30 (1). pp. 286-300. ISSN 0892-6638 Pribér, János Krisztián and Fónai, Fruzsina and Jakus, Péter and Rácz, Boglárka and Chinopoulos, Christos and Tretter, László and Gallyas, Ferenc and Sümegi, Balázs and Veres, Balázs (2015) Cyclophilin D disruption attenuates lipopolysaccharide-induced inflammatory response in primary mouse macrophages. BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, 93 (3). pp. 241-250. ISSN 0829-8211 Dobolyi, Arpád and Ostergaard, Elsebet and Bagó, Attila G. and Dóczi, Tamás and ...http://real.mtak.hu/view/creators/Chinopoulos=3AChristos=3A=3A.default.html
"Endotoxin recognition and signal transduction by the TLR4/MD2-complex" by Katherine A. Fitzgerald, Daniel C. Rowe et al.
Bacterial lipopolysaccharides are recognized in mammals by a receptor complex composed of CD14, Toll-like receptor (TLR)-4 and MD-2. Transduction of signaling is achieved following the recruitment of a combination of four Toll-interleukin-1 resistance (TIR)-domain-containing adapter molecules, which provide a structural platform enabling the recruitment and activation of downstream effectors essential for pathway-specific transcription factor activation and inflammatory gene expression.http://escholarship.umassmed.edu/gsbs_sp/293/