*  Ligation issues: post #1
I was doing a three way ligation and it didnt work for a long time so taking a new approach with just a simple ligation and ... Ligation issues - posted in Molecular Cloning: Hi, I have been having problems with my cloning for the past 2 months. ... I was doing a three way ligation and it didnt work for a long time so taking a new approach with just a simple ligation and ... I tried 16C water bath over night and also I have tried to leave the ligation at RT for about and hr and then leaving in the ...
  http://www.protocol-online.org/forums/topic/7467-ligation-issues/
*  Oligo ligation to PCR product: post #1
Oligo ligation to PCR product - posted in Molecular Biology: I am using a 2 % Agarose / 1 M urea gel for separation and ... Oligo ligation to PCR product. Started by anonymous, Mar 22 2001 10:00 PM ...
  http://www.protocol-online.org/forums/topic/472-oligo-ligation-to-pcr-product/
*  Difference between revisions of "Corum:T4 Ligation" - OpenWetWare
Retrieved from "https://openwetware.org/mediawiki/index.php?title=Corum:T4_Ligation&oldid=621385" ...
  https://openwetware.org/wiki/?title=Corum:T4_Ligation&diff=621385&oldid=621384
*  Ligation problem: post #1
So we tried to CIP treat vector before ligation and that did not work either. Then we decid... ... Ligation problem - posted in Molecular Biology: Hi All, I am trying to insert a fragment in the .THT vector, which was cut with ... Incubation conditions: digestion was carried at 37C for 2 hours and ligation was done at 16C overnight. Right after ligation I ... Depending on the quantity of my ligation mixture, I will either run it on a gel and gel extract my ligated product or just ...
  http://www.protocol-online.org/forums/topic/30612-ligation-problem/
*  NEBNext Adaptor Ligation (E6080) | NEB
Home Protocols NEBNext Adaptor Ligation (E6080) NEBNext Adaptor Ligation (E6080). Protocol. *After completion of the End Repair ...
  https://www.neb.com/protocols/1/01/01/nebnext-adaptor-ligation-e6080
*  Ligation SOE PCR fragment help: post #1
However, mysteriously after ligation, transformation and test restriction the fragment is not 1000 bp, but either 400, 500, 750 ... Ligation SOE PCR fragment help - posted in Molecular Cloning: Hello everyone, I am trying to clone a 1000 bp fragment, obtained ... Cloning - Ligation problem Started by cahyaprihatna, 01 Apr 2015 ligation, problem, cloning * 3 replies ... Also tagged with one or more of these keywords: ligation, SOEing PCR, shorter fragments. Protocols and Techniques Forums → ...
  http://www.protocol-online.org/forums/topic/24123-ligation-soe-pcr-fragment-help/
*  In Gel Ligation - Molecular Biology - BioForum
I have come across a protocol for in gel ligation, where the RE digested insert and vector are run on a low melting point gel, ... Hi I am having real trouble getting a couple of ligations to work and have been searching around for any protocols that may ... the fragments are excised and melted and used directly in the ligation reaction Has anyone used this method before? Does it ... For trouble ligations I find that the Bioline Quick stick ligase work well. Also if I have a PCR product that has been ...
  http://www.protocol-online.org/forums/topic/5307-in-gel-ligation/
*  Triple ligation not working: post #1
I treated the vector backbone with CIP and purified . Tried to religate the inserts with CIP treated vector but no ligation was ... after ligation I used to get a band of around 6Kb but after transformation none of the colony screened show the presence of the ... Triple ligation not working - posted in Molecular Cloning: Hi all, I am trying to ligate insert 1 ( Nco1-Sac1, 2180 bp), ... I add ATP (2x) to the ligation, and incubate o/n at 8'C. -Be gentle when mixing. -include a controls with an intact vector as a ...
  http://www.protocol-online.org/forums/topic/12334-triple-ligation-not-working/
*  Ligation
... ChenHA via methods%40net.bio.net (by hzhen from freeuk.com). Thu Jun 21 22:35:37 EST 2007 *Previous message: Ligation ... There are many variables in ligation (e.g. concentration and size of DNA, vector/insert ratio etc.), and this is the bit I'd ... Having good competent cells just makes life easier - I can do just a single ligation reaction for each construct (easier when I ... and I'd say having good competent cells is the main reason why I only need to do repeat ligation for a very small fraction of ...
  http://www.bio.net/bionet/mm/methods/2007-June/102307.html
*  Protein Ligation
... Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de Mon Jan 19 04:27:18 EST 1998 *Previous message: ...
  http://www.bio.net/bionet/mm/proteins/1998-January/006149.html
*  ligation/transformation
... schroeder at vzdmza.zdv.uni-mainz.de schroeder at vzdmza.zdv.uni-mainz.de Fri May 12 03:31:28 EST 1995 ... Ligations, when analyzed on a gel, seemed to work fine. When used for transformation only very few colonies are growing, which ...
  http://www.bio.net/bionet/mm/methods/1995-May/028290.html
*  Ligation failed
... newsnet customer customer at newsnet.com Sat May 6 09:19:09 EST 2006 *Previous message: Ligation failed ... Here is the ligation reaction: , , , , insert (12ng/ul) 2ul , , linearised vector (6ng/ul) 4ul , , 10X lig. buffer 1ul , , T4 ... Total DNA in ligation reaction is 48ng. , , insert:vector ratio is 1:1 in accordance to Roche T4 ligase information , , sheet ... Both the insert and the vector are large , enough that the intramolecular ligation has a higher chance than the , ...
  http://www.bio.net/bionet/mm/methods/2006-May/100520.html
*  RNA ligation
... Nick Theodorakis via methods%40net.bio.net (by nick.theodorakis from gmail.com). Wed Nov 18 20:54:25 EST 2009 * ...
  http://www.bio.net/bionet/mm/methods/2009-November/104406.html
*  ligation problem
... NJ njuni at poppy.ocn.ne.jp Wed May 23 00:30:21 EST 2001 *Previous message: ligation problem ... Control ligations include vector with ligase and no insert , , , , RESULT : NO CLONES , , I hope u have some ideas :) , , ... Checked purity and yield of vecotr and insert by running on gel and ligation , setup with excess insert over vecotr at 15C for ...
  http://www.bio.net/bionet/mm/methods/2001-May/088856.html
*  ligation problems. Help
... M K Bennett pamkb at mail.bris.ac.uk Tue Apr 25 06:48:24 EST 1995 *Previous message: ligation problems ... two-way ligation to work. My vector is : 6.5 kb, with two homologous repeats (the LTRs in pLXSN), and the : insert is 3.5 kb (b ... I have tried transforming ligations into : HB101 and XL1Blue, but keep obtaining colonies containing either : vector alone, or ...
  http://www.bio.net/bionet/mm/methods/1995-April/027625.html
*  EWTN.com - Bilateral tubal ligation
Tubal ligation is never morally permissible for the direct intention of preventing pregnancy. This is a moral principle which ... Automatically this third one should be by c section accompanied By a bilateral tubal ligation as future pregnancies may be ...
  http://www.ewtn.com/v/experts/showmessage.asp?number=633137&Pg=Forum10&Pgnu=1&recnu=21
*  Peptide Ligation | NEB
The IPL reaction allows ligation of a synthetic peptide or a protein with an N-terminal cysteine residue to the C-terminus of ... Peptide Ligation. Product Listing Application Overview The IPL reaction allows the ligation of a synthetic peptide or a protein ... The transitory ligation product then undergoes a spontaneous S-N acyl rearrangement from a thioester to a stable peptide bond. ... Home Applications Protein Analysis & Tools Protein Labeling Peptide Ligation ...
  https://www.neb.com/applications/protein-analysis-and-tools/protein-labeling/peptide-ligation
*  Vein ligation and stripping
This is the ligation part of the operation. The second step is where a thin flexible length of wire is inserted into the bottom ... Vein ligation and stripping This is the most common form of surgery for varicose veins. Vein stripping has been performed for a ... Recovery after vein ligation and stripping A surgical dressing or bandage is applied to the treated area. You will have to wear ... Advantages of vein ligation and stripping The main benefit from this surgery is that of removal of the offending vein. ...
  http://www.medic8.com/cosmetic-surgery/varicose-veins/vein-ligation-and-stripping.html
*  ligation of PCR products
... Jeffrey Lawrence jlawrenc at vms.cis.pitt.edu Mon Oct 7 11:46:55 EST 1996 *Previous message: ligation ... overhang will also interfere with ligations. this base can be removed with T4 DNA polymerase (and its ever-so-handy exo ...
  http://www.bio.net/bionet/mm/methods/1996-October/049939.html
*  Blunt end ligation problem
... Ian A. York iayork at panix.com Wed Dec 16 10:28:20 EST 1998 *Previous message: Blunt end ligation ... If you don't dilute it, and use too much CIP, the ends get screwy and you get very inefficient ligation. When I had my tiny ...
  http://www.bio.net/bionet/mm/methods/1998-December/072414.html
*  ligation of BIG inserts
I'd be happy if somebody could give me a hint about ligation and ,transformation procedures (any addition to standard ligation ... ligation of BIG inserts. Dr. Duncan Clark duncan at genesys.demon.co.uk Mon Mar 30 03:20:37 EST 1998 *Previous message: ... It's probably not the ligation that is the problem but the capacity of the E.coli for such a high copy vector with that size of ...
  http://www.bio.net/bionet/mm/methods/1998-March/065865.html
*  Tubal ligation reversal Risks - Mayo Clinic
A tubal ligation reversal is a procedure to restore fertility after a woman has had a tubal ligation - a procedure that cuts or ... A tubal ligation reversal may allow a woman who had a tubal ligation to get pregnant without further medical assistance. There ... A tubal ligation reversal is more likely to be successful if there is still a large proportion of healthy tube. If your ... Tubal ligation reversal is abdominal surgery, which carries a risk of infection, bleeding and injury to nearby organs, as well ...
  https://www.mayoclinic.org/tests-procedures/tubal-ligation-reversal/basics/risks/prc-20020246?p=1
*  Pregnancy after Tubal Ligation?? | DailyStrength
my sister had a tubal ligation two years ago and this past summer she got pregnant, but she ended up having a miscarrage. so it ...
  https://www.dailystrength.org/group/pregnancy/discussion/pregnancy-after-tubal-ligation
*  HELP WANTED: Trouble with ligation
... Greg Nattrass gnattras at waite.adelaide.edu.au Fri Sep 19 20:13:57 EST 1997 *Previous ... In the past I have had two main problems with failed ligation reactions. The first appeared to involve a seasonal decrease in ... The more common problem is the carry over of CIP into the ligation reaction. This effectively removes the phosphates from your ... insert, thus no ligation and no transformants. I routinely use no more than 1unit CIP/pmole DNA ends (routinely 0.2unit/ ...
  http://www.bio.net/bionet/mm/methods/1997-September/061045.html