*  how to confirm ligation is successful - Molecular Cloning
how to confirm ligation is successful - (Mar/01/2006 ). Hi.Could anyone tell me what method can i use in order to know my ... If its simple ligation and therefore expect positives you may need the plasmid so better make minipreps. You can check by ... I agree also, if its a complicated ligation and you expect to have few positive clones, then it is better to screen a lot of ... Anyway, you may need to sequence your plasmid at the end, depending what you need it for, to check correct ligation sites, etc ...
  http://www.protocol-online.org/biology-forums/posts/14130.html
*  Ligation issues: post #1
I was doing a three way ligation and it didnt work for a long time so taking a new approach with just a simple ligation and ... Ligation issues - posted in Molecular Cloning: Hi, I have been having problems with my cloning for the past 2 months. ... I was doing a three way ligation and it didnt work for a long time so taking a new approach with just a simple ligation and ... I tried 16C water bath over night and also I have tried to leave the ligation at RT for about and hr and then leaving in the ...
  http://www.protocol-online.org/forums/topic/7467-ligation-issues/
*  Oligo ligation to PCR product: post #1
Oligo ligation to PCR product - posted in Molecular Biology: I am using a 2 % Agarose / 1 M urea gel for separation and ... Oligo ligation to PCR product. Started by anonymous, Mar 22 2001 10:00 PM ...
  http://www.protocol-online.org/forums/topic/472-oligo-ligation-to-pcr-product/
*  Difference between revisions of "Corum:T4 Ligation" - OpenWetWare
Retrieved from "https://openwetware.org/mediawiki/index.php?title=Corum:T4_Ligation&oldid=621385" ...
  https://openwetware.org/wiki/?title=Corum:T4_Ligation&diff=621385&oldid=621384
*  Difference between revisions of "Jamesh008:consensus DNA ligation protocol" - OpenWetWare
Endy:DNA ligation using T4 DNA ligase -- Using T4 DNA Ligase Knight:DNA ligation using NEB Quick Ligation Kit -- 5min ligation ... Silver:Ligation -- A protocol using the Roche Kit. BE.109:DNA ligation -- A ligation protocol for classroom use in a laboratory ... If you are having trouble with your ligation, NEB offers FAQ's (Quick Ligation T4 DNA ligase) to help. ... Add 1 μL ligation buffer to the tube. Vortex buffer before pipetting to ensure that it is well-mixed. Remember that the buffer ...
  https://openwetware.org/wiki/?title=Jamesh008:consensus_DNA_ligation_protocol&diff=85298&oldid=84474
*  Unable to obtain colonies from PCR-insert + vector ligation - Molecular Cloning
Also tried ligation with a regular T4 DNA ligase (not the quick ligation kit), didn't work. I think maybe the problem is in the ... I tried getting rid of the PEG in the quick ligation mix using spin columns, and then transforming using the purified ligation ... Unable to obtain colonies from PCR-insert + vector ligation - (Jun/14/2006 ). I am using a pET-19b (5.5kb) and a 1.4kb insert, ... Last time, which is more than a year ago, I used the quick ligation kit (NEB), they suggested to heat shock cells and not ...
  http://www.protocol-online.org/biology-forums/posts/17293.html
*  Ligation problem: post #1
So we tried to CIP treat vector before ligation and that did not work either. Then we decid... ... Ligation problem - posted in Molecular Biology: Hi All, I am trying to insert a fragment in the .THT vector, which was cut with ... Incubation conditions: digestion was carried at 37C for 2 hours and ligation was done at 16C overnight. Right after ligation I ... Depending on the quantity of my ligation mixture, I will either run it on a gel and gel extract my ligated product or just ...
  http://www.protocol-online.org/forums/topic/30612-ligation-problem/
*  NEBNext Adaptor Ligation (E6080) | NEB
Home Protocols NEBNext Adaptor Ligation (E6080) NEBNext Adaptor Ligation (E6080). Protocol. *After completion of the End Repair ...
  https://www.neb.com/protocols/1/01/01/nebnext-adaptor-ligation-e6080
*  Ligation SOE PCR fragment help: post #1
However, mysteriously after ligation, transformation and test restriction the fragment is not 1000 bp, but either 400, 500, 750 ... Ligation SOE PCR fragment help - posted in Molecular Cloning: Hello everyone, I am trying to clone a 1000 bp fragment, obtained ... Cloning - Ligation problem Started by cahyaprihatna, 01 Apr 2015 ligation, problem, cloning * 3 replies ... Also tagged with one or more of these keywords: ligation, SOEing PCR, shorter fragments. Protocols and Techniques Forums → ...
  http://www.protocol-online.org/forums/topic/24123-ligation-soe-pcr-fragment-help/
*  Afl II ligations... any suggestions?
... Name fred at here.there.com Tue Mar 3 19:48:04 EST 1998 *Previous message: Tons of FREE ... I've tried ligation +/- PEG, high and low amounts of DNA, at 12 degrees C and room temp., and high and low ligase (400 and 2000 ...
  http://www.bio.net/bionet/mm/methods/1998-March/065113.html
*  In Gel Ligation - Molecular Biology - BioForum
I have come across a protocol for in gel ligation, where the RE digested insert and vector are run on a low melting point gel, ... Hi I am having real trouble getting a couple of ligations to work and have been searching around for any protocols that may ... the fragments are excised and melted and used directly in the ligation reaction Has anyone used this method before? Does it ... For trouble ligations I find that the Bioline Quick stick ligase work well. Also if I have a PCR product that has been ...
  http://www.protocol-online.org/forums/topic/5307-in-gel-ligation/
*  RNA Whole-Mount In situ Hybridisation Proximity Ligation Assay (rISH-PLA) (Myers and Scarlett labs) - Xenbase
6) The oocytes were then incubated with the ligation solution (20 ul of 5 x ligation stock, 77.5 ul of Sigma H2O (SH2O) and 2.5 ... 8) Excess unbound ligation solution was removed by washing 3 times for 5 min at RT on a rocker with 1ml of 1 x Wash Buffer A. ... RNA Whole-Mount In situ Hybridisation Proximity Ligation Assay (rISH-PLA) (Myers and Scarlett labs). From Xenbase ... Retrieved from "http://wiki.xenbase.org/xenwiki/index.php?title=RNA_Whole-Mount_In_situ_Hybridisation_Proximity_Ligation_Assay ...
  http://wiki.xenbase.org/xenwiki/index.php/RNA_Whole-Mount_In_situ_Hybridisation_Proximity_Ligation_Assay_
*  Triple ligation not working: post #1
I treated the vector backbone with CIP and purified . Tried to religate the inserts with CIP treated vector but no ligation was ... after ligation I used to get a band of around 6Kb but after transformation none of the colony screened show the presence of the ... Triple ligation not working - posted in Molecular Cloning: Hi all, I am trying to ligate insert 1 ( Nco1-Sac1, 2180 bp), ... I add ATP (2x) to the ligation, and incubate o/n at 8'C. -Be gentle when mixing. -include a controls with an intact vector as a ...
  http://www.protocol-online.org/forums/topic/12334-triple-ligation-not-working/
*  Endoscopic Ligation Compared with Sclerotherapy for the Treatment of Bleeding Esophageal Varices | Annals of Internal Medicine ...
Complications were less common in the ligation group: fewer patients in the ligation group had esophageal strictures (0 of 38 ... Endoscopic Ligation Compared with Sclerotherapy for Treatment of Esophageal Variceal Bleeding: A Meta-Analysis Annals of ... Endoscopic Ligation Compared with Sclerotherapy for the Treatment of Bleeding Esophageal Varices Loren Laine, MD; Hussein M. El ... Ligation is a viable alternative to sclerotherapy and may have some advantages as a treatment for bleeding esophageal varices. ...
  http://annals.org/aim/article-abstract/706486/endoscopic-ligation-compared-sclerotherapy-treatment-bleeding-esophageal-varices
*  Ligation - Wikipedia
Ligation may refer to: Ligation (molecular biology), the covalent linking of two ends of DNA or RNA molecules, most commonly ... the production of peptides from amino acids Tubal ligation, a method of female sterilization Rubber band ligation, a treatment ... In medicine, the making of a Ligature (medicine) (tie) Chemical ligation, ...
  https://en.wikipedia.org/wiki/Ligation
*  KAHA Ligation - Wikipedia
It is an alternative to the Native Chemical Ligation (NCL). An α-ketoacid at the C-terminus of one peptide fragment reacts with ... The α-Ketoacid-Hydroxylamine (KAHA) Amide-Forming Ligation is a chemical reaction that is used to join two unprotected ... Bode, Jeffrey W.; Fox, Ryan M.; Baucom, Kyle D. (2006). "Chemoselective Amide Ligations by Decarboxylative Condensations ofN- ... Pusterla, Ivano; Bode, Jeffrey W. (2012). "The Mechanism of the α-Ketoacid-Hydroxylamine Amide-Forming Ligation". Angewandte ...
  https://en.wikipedia.org/wiki/KAHA_Ligation
*  Tubal ligation - Wikipedia
Pomeroy tubal ligation In this method of tubal ligation, a loop of tube is "strangled" with a suture. Usually, the loop is cut ... Essure tubal ligation In this method of tubal ligation, two small metal and fiber coils are placed in the fallopian tubes. ... Adiana tubal ligation In this method of tubal ligation, two small silicone pieces were placed in the fallopian tubes. During ... Tubal ligation is usually done in a hospital operating-room setting. A tubal ligation is approximately 99% effective in the ...
  https://en.wikipedia.org/wiki/Tubal_ligation
*  Chemical ligation - Wikipedia
In native chemical ligation, an unprotected peptide-thioester reacts with a Cys-peptide to give a ligation product with a ... The Staudinger ligation continues to be developed and has not yet found widespread use. Ser/Thr ligation (STL) was introduced ... The original chemical ligation methods involved the formation of a non-native bond at the ligation site. Subsequently, native ... Native chemical ligation relies on the presence of a cysteine residue at the ligation site. Methods using removable auxiliary ...
  https://en.wikipedia.org/wiki/Chemical_ligation
*  Sequencing by ligation - Wikipedia
Sequencing by ligation is a DNA sequencing method that uses the enzyme DNA ligase to identify the nucleotide present at a given ... Sequencing by ligation relies upon the sensitivity of DNA ligase for base-pairing mismatches. The target molecule to be ... Sequencing by ligation can proceed in either direction (either 5'-3' or 3'-5') depending on which end of the probe ... This sequencing by ligation method has been reported to have problems sequencing palindromic sequences. Sequencing by ...
  https://en.wikipedia.org/wiki/Sequencing_by_ligation
*  Proximity ligation assay - Wikipedia
Proximity ligation assay (in situ PLA) is a technology that extends the capabilities of traditional immunoassays to include ... Roussis IM, Guille M, Myers FA, Scarlett GP (2016). "RNA Whole-Mount In situ Hybridisation Proximity Ligation Assay (rISH-PLA ... "Proximity ligation assays for sensitive and specific protein analyses". Analytical Biochemistry. 345 (1): 2-9. doi:10.1016/j.ab ... "Cytokine detection by antibody-based proximity ligation". Proceedings of the National Academy of Sciences of the United States ...
  https://en.wikipedia.org/wiki/Proximity_ligation_assay
*  Rubber band ligation - Wikipedia
The CRH O'Regan ligation system also eliminates the use of forceps. It is much more expensive on a per-case basis than the ... Rubber band ligation is a popular procedure for the treatment of hemorrhoids, as it involves a much lower risk of pain than ... With rubber band ligation, a small band is applied to the base of the hemorrhoid, stopping the blood supply to the hemorrhoidal ... Ligation of hemorrhoids was first recorded by Hippocrates in 460 BC, who wrote about using thread to tie off hemorrhoids. In ...
  https://en.wikipedia.org/wiki/Rubber_band_ligation
*  Ligation (molecular biology) - Wikipedia
TA ligation is therefore a form of sticky end ligation. Blunt-ended vectors may be turned into vector for TA ligation with ... Ligation is complicated by the fact that the desired ligation products for most ligation reactions should be between two ... Blunt-end ligation is much less efficient than sticky end ligation, so a higher concentration of ligase is used in blunt-end ... For the ligation reaction to proceed efficiently, the ends should be stably annealed, and in ligation experiments, the Tm of ...
  https://en.wikipedia.org/wiki/Ligation_(molecular_biology)
*  Ligation-independent cloning - Wikipedia
Ligation-independent cloning (LIC) is a form of molecular cloning that is able to be performed without the use of restriction ... "Get Your Clone 90% Of The Time with Ligation Independent Cloning". Bitesize Bio. Retrieved 2012-05-09. Haun, RS; Serventi, IM; ... "Ligation Independent Cloning (LIC)". New England BioLabs (NEB). Retrieved 15 January 2016. " ... Moss, J (1992). "Rapid, reliable ligation-independent cloning of PCR products using modified plasmid vectors". BioTechniques. ...
  https://en.wikipedia.org/wiki/Ligation-independent_cloning
*  Native chemical ligation - Wikipedia
Native chemical ligation or NCL is an important extension of the chemical ligation field, a concept for constructing a large ... Especially, NCL is the most powerful ligation method for synthesizing native backbone proteins or modified proteins of moderate ... size (i.e., small proteins< 200 AA). In native chemical ligation, the thiol group of an N-terminal cysteine residue of an ...
  https://en.wikipedia.org/wiki/Native_chemical_ligation
*  Ligation
... ChenHA via methods%40net.bio.net (by hzhen from freeuk.com). Thu Jun 21 22:35:37 EST 2007 *Previous message: Ligation ... There are many variables in ligation (e.g. concentration and size of DNA, vector/insert ratio etc.), and this is the bit I'd ... Having good competent cells just makes life easier - I can do just a single ligation reaction for each construct (easier when I ... and I'd say having good competent cells is the main reason why I only need to do repeat ligation for a very small fraction of ...
  http://www.bio.net/bionet/mm/methods/2007-June/102307.html