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A synaptic Ras-GTPase activating protein (p135 SynGAP) inhibited by CaM kinase II. Neuron 20: 895-904. ... which has been shown to mediate synaptic targeting of the PSD95β isoform through protein-protein interactions (Chetkovich et al ... To test antibody specificity, transfected COS-7 cells were fixed for immunostaining, and transfected QT6 cells were lysed for ... the Rho-GTPase interacting protein Citron (Zhang et al., 1999), and the synaptic Ras-GTPase activating protein SynGAP (Chen et ...
  http://www.jneurosci.org/content/24/2/378
*  CDC42 binds PAK4 via an extended GTPase-effector interface | PNAS
2015) Signaling, regulation, and specificity of the type II p21-activated kinases. J Biol Chem 290:12975-12983. ... such as specificity (18), probing the interactions of full-length proteins could potentially help shed light on how GTPases ... kinase autoinhibition takes place upon RAC1/CDC42 binding to type I PAKs and is found in other GTPase-kinase pairs such as RAS- ... a kinase domain and a CRIB-GTPase complex "domain," connected by the flexible linker. As protein-protein interaction surfaces ...
  http://www.pnas.org/content/115/3/531
*  Regulation of Dendritic Spines, Spatial Memory, and Embryonic Development by the TANC Family of PSD-95-Interacting Proteins |...
... and Nck-interacting kinase), which belong to the STE20 family of mitogen-activated protein kinase kinase kinase kinases (MAP4Ks ... a Ras family small GTPase implicated in the negative regulation of dendritic spines and synaptic AMPA receptors (Pak et al., ... TANC1 is a PSD-95-interacting synaptic protein that contains multiple domains for protein-protein interactions but whose ... Specific antibodies were affinity-purified using SulfoLink columns (Pierce). The specificity of the TANC antibodies (1609 and ...
  http://www.jneurosci.org/content/30/45/15102
*  EVI5L - Wikipedia
... proteins, and functions as a GTPase-activating protein (GAP) with a broad specificity. Measurement of in vitro Rab-GAP activity ... serine/threonine-protein kinase 2) SRPK2 (serine/threonine-protein kinase 2) Function Jenkins D, Beales PL, Wilkie AO (May 2012 ... while TPR mediates protein-protein interactions and the assembly of multi protein complexes. These three domains are highly ... EVI5L is a member of the Ras superfamily of monomeric guanine nucleotide-binding (G) ...
  https://en.wikipedia.org/wiki/EVI5L
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... their interactions with inhibitory proteins and with activating proteins that regulate the exchange of GDP for GTP. The Ras ... The Rho GTPase family affects various cellular processes like proliferation and apoptosis. Notably, the Rho kinase inhibitor Y- ... Potentiated Raf principally propagates signaling by phosphorylating the two dual-specificity MAPK kinases: MEK1 and MEK2 (Fig. ... phosphatidylinositol-3 kinase; PKC, protein kinase C; RIN1, Ras and Rab interactor 1; RKIP, Raf kinase inhibitor protein; SOS, ...
  http://mct.aacrjournals.org/content/6/3/811
*  Active GTPases in Neuronal Cells | Thermo Fisher Scientific - SA
... and activates Ras GTPase at the membrane. Additional Ras and Rho GTPases are activated after signaling of Ras via PI3 kinase, ... Differentiated cells have highly specified roles guided by cascades of protein interactions. In these cascades, small GTPases ... more optimization of the effector binding domains is necessary for better specificity and lower background. ... The Ras GTPase family acts as membrane-associated signal transducers; the Rho GTPase family regulates actin and microtubule ...
  https://www.thermofisher.com/sa/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-biology-application-notes/active-gtpases-neuronal-cells.html
*  MAP kinase pathways | Journal of Cell Science
... that activate the Rho1 GTPase, which activates protein kinase C (PKC) 1, a MAPKKKK for the pathway (Hohmann, 2002). ... Pathway specificity is regulated at several levels, including kinase-kinase and kinase-substrate interactions, colocalization ... This is facilitated by the scaffold protein KSR, which links the three tiers of kinases to Ras. Other scaffold proteins can ... Roux, P. P. and Blenis, J. (2004). ERK and p38 MAPK-activated protein kinases: a family of protein kinases with diverse ...
  http://jcs.biologists.org/content/118/16/3569.full
*  SMART: ANK domain annotation
Like Ras, ARFs are active in their GTP-bound form, and their duration of activity is controlled by GTPase-activating proteins ( ... They are involved in protein-protein interactions. The core of the repeat seems to be an helix-loop-helix structure. ... PAPbeta, a protein that binds to and is phosphorylated by the non-receptor tyrosine kinase PYK2, contains several modular ... Contacts occur in discontinuous patches, suggesting a combinatorial quality for ankyrin repeat specificity. The first two ...
  http://smart.embl.de/smart/do_annotation.pl?DOMAIN=ANK&START=708&END=737&E_VALUE=3.95e1&TYPE=SMART&BLAST=LKNTMLERACDQNNSIMVECLLLLGADANQ
*  SMART: Secondary literature for RAS domain
GTP activated Ras interacts directly with the Raf protein to recruit the MAP kinases and their subordinates. Attachment of Ras ... The mutant proteins show reduced GTPase rates, which are not stimulated by the GTPase-activating protein GAP. p21(S17A) has ... are targets of Ras, giving rise to different effects in the cell cycle progression. These Ras-mediated interactions are an ... p21 has a preference for the Sp isomers of GDP alpha S and GTP alpha S. It has low specificity for the Sp isomer of GTP beta S ...
  http://smart.embl.de/smart/show_secondary.cgi?domain=RAS
*  G-protein signaling - Regulation of CDC42 activity Pathway Map - PrimePCR | Life Science | Bio-Rad
Rho GTPase activating protein 5 (RhoGAP5) GAP activity is abrogated by RAS p21 protein activator (GTPase activating protein) 1 ... Dock6, a Dock-C subfamily guanine nucleotide exchanger, has the dual specificity for Rac1 and Cdc42 and regulates neurite ... A crystallographic view of interactions between Dbs and Cdc42: PH domain-assisted guanine nucleotide exchange. The EMBO journal ... GEF activity of Dbl towards CDC42 is enhanced upon Tyrosine kinase, non-receptor, 2 (ACK1)-dependent phosphorylation [15], [16 ...
  http://www.bio-rad.com/en-us/prime-pcr-assays/pathway/g-protein-signaling-regulation-cdc42-activity
*  Ultrasensitivity - Wikipedia
... and GTPase activating proteins (GAPs). Computational studies on the switching behavior of GTPases have revealed that the GTPase ... "An ultrasensitive Ca2+/calmodulin-dependent protein kinase II-protein phosphatase 1 switch facilitates specificity in ... Bashor, C. J.; Helman, N. C.; Yan, S.; Lim, W. A. (2008). "Using Engineered Scaffold Interactions to Reshape MAP Kinase Pathway ... such as Ran and Ras, can exist in either a GTP-bound form (active) or a GDP-bound form (inactive), and the conversion between ...
  https://en.wikipedia.org/wiki/Ultrasensitivity
*  Advances in the Development of Cancer Therapeutics Directed against the RAS-Mitogen-Activated Protein Kinase Pathway | Clinical...
... conjunction with dual specificity kinase capability is consistent with these proteins occupying a strategic position in the RAS ... Structural alterations in the upstream GTPase RAS occur in ∼25% of human cancers and confer them with the ability to relay ... viable drug development candidates largely because of the inherent difficulties of disrupting protein-protein interactions with ... a potent inhibitor of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase 1/2 kinases: mechanism of ...
  http://clincancerres.aacrjournals.org/content/14/12/3651.long
*  RAB8A - Wikipedia
... the GTP-binding protein rab8 interacts with a stress-activated protein kinase". Proceedings of the National Academy of Sciences ... Ras-related protein Rab-8A is a protein that in humans is encoded by the RAB8A gene. The protein encoded by this gene is a ... "General role of GDP dissociation inhibitor 2 in membrane release of Rab proteins: modulations of its functional interactions by ... Zahraoui A, Joberty G, Arpin M, Fontaine JJ, Hellio R, Tavitian A, Louvard D (Jan 1994). "A small rab GTPase is distributed in ...
  https://en.wikipedia.org/wiki/RAB8A
*  RASGRF1 (Ras protein-specific guanine nucleotide-releasing factor 1)
Ras protein-specific guanine nucleotide-releasing factor 1), Authors: Fernando Calvo, Piero Crespo. Published in: Atlas Genet ... In vitro, Ras-GRF1 can activate H-Ras, K-Ras and N-Ras GTPases, but in vivo, its specificity is reduced to H-Ras, probably as a ... Differential response of the Ras exchange factor, Ras-GRF to tyrosine kinase and G protein mediated signals.. ... positive regulation of GTPase activity regulation of Ras protein signal transduction positive regulation of Ras protein signal ...
  http://atlasgeneticsoncology.org/Genes/GC_RASGRF1.html
*  Gene Report for G00001924 - Genes2Cognition Neuroscience Research Programme
... ras) GTPase-activating protein and COOH-terminal Grb2. Distinct sites in the proline-rich COOH-terminal region of synapsin I ... Phosphorylation of amphiphysin I by minibrain kinase/dual-specificity tyrosine phosphorylation-regulated kinase, a kinase ... is a non-receptor tyrosine kinase that activates Src family kinases via SH2- and SH3-mediated interactions. Specific FAK ... calmodulin-dependent protein kinase II or mitogen-activated protein kinase modulated the binding to the SH3 domains of ...
  http://www.genes2cognition.org/db/Gene/G00001924
*  SYNGAP1 Gene - GeneCards | SYGP1 Protein | SYGP1 Antibody
Protein Coding), Synaptic Ras GTPase Activating Protein 1, including: function, proteins, disorders, pathways, orthologs, and ... Exhibits dual GTPase-activating specificity for Ras and Rap. May be involved in certain forms of brain injury, leading to long- ... Phosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption. ... Synaptic Ras GTPase activating protein 1 homolog (Rat) (B1AZX6_HUMAN). *Synaptic Ras GTPase-activating protein 1 (SYGP1_HUMAN) ...
  http://www.genecards.org/cgi-bin/carddisp.pl?gene=SYNGAP1&simap=3
*  Targeting of Mitogen-Activated Protein Kinases and Phosphatidylinositol 3 Kinase Inhibits Hepatocyte Growth Factor/Scatter...
HGF/SF had been shown to activate Ras,20 which can interact with GTPase-activating proteins (GAP), Grb2 proteins, or Sos. ... Davies SP, Reddy H, Cohen P. Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem J. ... Purification of scatter factor, a fibroblast-derived basic protein that modulates epithelial interactions and movement. Proc ... Targeting of Mitogen-Activated Protein Kinases and Phosphatidylinositol 3 Kinase Inhibits Hepatocyte Growth Factor/Scatter ...
  http://circ.ahajournals.org/content/107/23/2955
*  MAPKAP1 - Target of rapamycin complex 2 subunit MAPKAP1 - Homo sapiens (Human) - MAPKAP1 gene & protein
mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function ... MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization ... upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide ... Ras GTPase binding Source: UniProtKB ,p>Inferred from Direct Assay,/p> ,p>Used to indicate a direct assay for the function, ...
  http://www.uniprot.org/uniprot/Q9BPZ7
*  PPSP: prediction of PK-specific phosphorylation site with Bayesian decision theory | BMC Bioinformatics | Full Text
Protein Kinase) groups. Compared with four existing tools Scansite, NetPhosK, KinasePhos and GPS, PPSP is more accurate and ... the intrinsic feature of substrates specificity is still elusive and remains to be delineated. In this work, we present a novel ... of proteins, phosphorylation plays essential regulatory roles in a broad spectrum of the biological processes. Although many ... Recently, protein Ras guanine-releasing factor 1 (RasGrf1, Q13972), a GTPase of the Ras and Rho family, has been proposed to be ...
  https://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-7-163
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Downregulation of the Ras-mitogen-activated protein kinase pathway by the EphB2 receptor tyrosine kinase is required for ephrin ... 2010). There is evidence that the inhibition is mediated by Ras GTPase-activating protein (RasGAP) (Elowe et al. 2001). RasGAP ... To study ephrin-BDNF interactions, we used a protocol in which a stable baseline was established for 3 min, followed by a 5-min ... Although the activity-dependent release of BDNF provides some spatial specificity, it cannot do so with the precision of cell- ...
  http://jn.physiology.org/content/105/5/2364
*  The GAPs, GEFs, and GDIs of heterotrimeric G-protein alpha subunits
GTPase activating specificity of RGS12 and binding specificity of an alternatively spliced PDZ (PSD-95/Dlg/ZO-1) domain. J Biol ... the potential for RGS12 to serve as a nexus for signaling from tyrosine kinases and G-proteins of both the Gα and Ras- ... The tetratricopeptide repeat: a structural motif mediating protein-protein interactions. Bioessays. 1999 ;21(11):932-9 ... GTPase-activating proteins for heterotrimeric G proteins: regulators of G protein signaling (RGS) and RGS-like proteins. Annu ...
  http://www.ijbs.com/v01p0051.htm
*  Tetraspanin CD151 Is a Negative Regulator of FcεRI-Mediated Mast Cell Activation | The Journal of Immunology
A potential link between FcεRI and the Ras/mitogen-activated protein kinase signaling pathway through SOS and Grb2. J. Biol. ... For instance, recruitment of inhibitory adaptor protein Dok-1 negatively regulates Ras by recruiting Ras GTPase or SHIP-1, ... Differential regulation of MAP kinase signalling by dual-specificity protein phosphatases. Oncogene 26: 3203-3213. ... Protein-protein interactions in the tetraspanin web. Physiology (Bethesda) 20: 218-224. ...
  http://www.jimmunol.org/content/195/4/1377
*  IJMS | Free Full-Text | MAPKs and Signal Transduction in the Control of Gastrointestinal Epithelial Cell Proliferation and...
In this review, we focus on ERK, JNK, p38 and ERK5, the major MAPKs, and their interactions with PI3K-Akt, TGFβ/Smad and Wnt/β- ... pathways are activated by several stimuli and transduce the signal inside cells, generating diverse responses including cell ... Biondi, R.M.; Nebreda, A.R. Signalling specificity of Ser/Thr protein kinases through docking-site-mediated interactions. ... for Ras, a GTPase that exists in humans in three forms: H-Ras, K-Ras and N-Ras. Therefore, SOS is relocated to the plasma ...
  http://www.mdpi.com/1422-0067/14/5/10143/htm
*  Identification of Proteins in the Postsynaptic Density Fraction by Mass Spectrometry | Journal of Neuroscience
1998) A synaptic Ras-GTPase activating protein (p135 SynGAP) inhibited by CaM Kinase II. Neuron 20:895-904. ... One criteria that we have used to assess the specificity of association of a protein with the PSD fraction is its enrichment in ... 1998) Subunit composition, protein interactions, and structures of the mammalian brain sec6/8 complex and septin filaments. ... a ras GTPase-activating protein phosphorylated by CaMKII (Chen et al., 1998). Other prominent PSD proteins are scaffold ...
  http://www.jneurosci.org/content/20/11/4069
*  Phosphoinositide phospholipase C - Wikipedia
... where it recruits protein kinase C (PKC), which becomes activated in conjunction with binding calcium ions. This results in a ... this specificity helps tether the enzyme tightly to the plasma membrane in order to access substrate through ionic interactions ... PLC-ε (230-260kDa ) is activated by Ras and Rho GTPases. PLC-ζ (75kDa) is thought to play an important role in vertebrate ... Members of the Rho GTPase family (e.g., Rac1, Rac2, Rac3, and cdc42) have been implicated in their activation by binding to an ...
  https://en.wikipedia.org/wiki/Phosphoinositide_phospholipase_C