*  Plus it
Mutations in the DDR2 kinase gene identify a novel therapeutic target in squamous cell lung cancer. Cancer Discov 2011;1:78-89 ... S2). Given recent reports that EPHA7 is a tumor suppressor (51), we also evaluated whether knockdown of EPHA7 might drive ... Generation and analysis of dasatinib-resistant cell lines. We cultured 2 cell lines, HCC-366 and NCI-H2286, previously shown to ... Cell Signaling Technologies), Mek (Cell Signaling Technologies), p-Mek (Ser217/221; Cell Signaling Technologies), Akt (Cell ...
  http://mct.aacrjournals.org/content/13/2/475
*  Oncogenic EGFR signaling cooperates with loss of tumor suppressor gene functions in gliomagenesis | PNAS
2007) Coactivation of receptor tyrosine kinases affects the response of tumor cells to targeted therapies. Science 318:287-290. ... were cultured ex vivo. The suitability of these cells to study signal transduction events was confirmed by comparing the levels ... The highly infiltrative nature of EGFR tumor cells is depicted. (Scale bar: 30 μM.) (D) Tumor cells (black arrowheads) are ... Western blot analysis of total cell extracts from GBM tumor cells expressing the indicated receptors. Cells were serum starved ...
  http://www.pnas.org/content/106/8/2712
*  Differential effects by the p21 CDK inhibitor on PCNA-dependent DNA replication and repair - CSHL Scientific Digital Repository
... carcinoma cell colon carcinoma dna replication excision repair human human cell priority journal tumor suppressor gene p53 Cell ... Tumor Cells, Cultured Mammalia Simiae Simian virus. Subjects:. bioinformatics , genomics and proteomics , genetics & nucleic ... Viral DNA-Directed DNA Polymerase Proliferating Cell Nuclear Antigen Protein Kinases Simian virus 40 Support, Non-U.S. Gov't ... In mammalian cells, DNA damage increases the levels of the nuclear tumour-suppressor p53, resulting in elevated synthesis of ...
  http://repository.cshl.edu/26489/
*  Yes-associated protein (YAP) transcriptional coactivator functions in balancing growth and differentiation in skin | PNAS
alpha}-Catenin Is a Tumor Suppressor That Controls Cell Accumulation by Regulating the Localization and Activity of the ... E) Precocious induction of late-differentiation genes by Yap KD in MKs cultured in low-Ca2+ medium. mRNAs were isolated from ... as well as the negative YAP regulator large tumor suppressor (LATS) kinase (3-6). ... surrounding these Mx-like cells were some cells with melanin granules, typical of hair shaft precursor cells, and other cells ...
  http://www.pnas.org/content/108/6/2270
*  Nutrients | Free Full-Text | Selenium in Bone Health: Roles in Antioxidant Protection and Cell Proliferation | HTML
Such findings may relate to roles of Se in antioxidant protection, enhanced immune surveillance and modulation of cell ... In that model, Se treatment increased the expression of humoral defense and tumor suppressor-related genes while decreasing the ... Jurkat and HL60 cells) were cultured in a Se-deficient, serum-free medium, GPX and TRR activities were low and cells did not ... cell cycle arrest in G1 phase and apoptosis via the extracellular-regulated kinase 1/2 pathway and other cancer signaling genes ...
  http://www.mdpi.com/2072-6643/5/1/97/htm
*  JCI - Welcome
... and induced the simultaneous CRISPR/Cas9-mediated knockout of 3 tumor-suppressor genes. Intramuscular injection of MuLE viruses ... By transducing cultured primary mouse cells with single MuLE lentiviruses, we engineered tumors containing up to 5 different ... together with combinations of knockdowns of the tumor suppressors cyclin-dependent kinase inhibitor 2A (. Cdkn2a. ), ... Adoptive transfer of tumor-reactive T cells can successfully reduce tumor burden; however, in rare cases, lethal on-target/off- ...
  https://www.jci.org/tags/4
*  Cancer Cell Metabolism | The New York Academy of Sciences
There is resurgent interest in cancer cell metabolism as researchers seek to understand how metabolic pathways are altered in ... The most common genetic mutations that drive cancers are in proto-oncogenes and tumor suppressor genes, which regulate pathways ... Cells cultured under hypoxic conditions, which might mimic those experienced by cancer cells in a poorly vascularized tumor, ... which targeted the brain-type creatine kinase (CKB). Further study revealed that this kinase is secreted from cells and then ...
  https://www.nyas.org/ebriefings/cancer-cell-metabolism/
*  CASP9 | Cancer Genetics Web
... and induced the simultaneous CRISPR/Cas9-mediated knockout of 3 tumor-suppressor genes. Intramuscular injection of MuLE viruses ... PMCs were co-cultured with the high TGF-β1-expressing lung cancer cell line A549 and with various TGF-β1 signaling inhibitors. ... Furthermore, obovatol activated the mitogen-activated protein kinase (MAPK) signaling pathway [c-Jun N-terminal kinase (JNK), ... cells by MTT assay and also increased the number of ethidium homodimer positively stained cells in A549 NSCLC cells. Also, cell ...
  http://www.cancerindex.org/geneweb/CASP9.htm
*  Adenomatous polyposis coli (Apc) tumor suppressor gene as a multifunctional gene | SpringerLink
The Apc gene product (APC), basically a cytoplasmic protein, blocks cell cycle ... gene is mutated in familial adenomatous polyposis and in sporadic colorectal tumors. ... The discs-large tumor suppressor gene of Drosophila encodes a guanylate kinase homolog localized at septate junctions. Cell 66 ... Shimomura A, Kohu K, Akiyama T, Senda T (2005) Subcellular localization of the tumor suppressor APC in developing cultured ...
  https://link.springer.com/article/10.1111%2Fj.1447-073x.2005.00106.x
*  PML is required for p73 transcriptional and proapoptoti | Open-i
Cells, Cultured. *Genes, Tumor Suppressor. *Humans. *Mitogen-Activated Protein Kinases/physiology. *Receptors, Retinoic Acid/ ... p73 has been identified recently as a structural and functional homologue of the tumor suppressor p53. Here, we report that p73 ... p73 has been identified recently as a structural and functional homologue of the tumor suppressor p53. Here, we report that p73 ... Indeed, p73α transcriptional activity was diminished up to 65% in Pml−/− cells (Fig. 7 A and not depicted). In agreement with ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC2211783_20031943f7&req=4
*  Rho Kinase Regulates Schwann Cell Myelination and Formation of Associated Axonal Domains | Journal of Neuroscience
Scherer SS, Gutmann DH (1996) Expression of the neurofibromatosis 2 tumor suppressor gene product, merlin, in Schwann cells. J ... Kachar B, Behar T, Dubois-Dalcq M (1986) Cell shape and motility of oligodendrocytes cultured without neurons. Cell Tissue Res ... Riento K, Ridley AJ (2003) Rocks: multifunctional kinases in cell behaviour. Nat Rev Mol Cell Biol 4: 446-456. ... Actin plays a role in both changes in cell shape and gene-expression associated with Schwann cell myelination. J Neurosci 17: ...
  http://www.jneurosci.org/content/24/16/3953.long
*  Oncogenic KRAS Desensitizes Colorectal Tumor Cells to Epidermal Growth Factor Receptor Inhibition and Activation
... including epithelial cells [1]. Most colorectal tumors are initiated by inactivating mutations in the tumor suppressor gene APC ... extracellular signal-regulated kinase. MAPK. mitogen-activated protein kinase. MEK. MAPK/ERK kinase. PI3K. phosphatidylinositol ... All these cell lines were cultured in Dulbecco's modified Eagle medium (DMEM; Dulbecco, ICN Pharmaceuticals, Zoetermeer, The ... EGF stimulation of HCT116 and HKH2 cells showed that the immediate early response gene c-FOS was readily induced in HKH2 cells ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2887497/
*  Betulinic Acid-Induced Apoptosis in Glioma Cells: A Sequential Requirement for New Protein Synthesis, Formation of Reactive...
1995) Tumor suppressor p53 is a direct transcriptional activator of the human BAX gene. Cell 80:293-299. ... LN-308 cells do not express p53 protein (Van Meir et al., 1994). The human glioma cell lines were cultured in Dulbecco's ... In regard to p21, this protein inhibits cyclin-dependent kinase (cdk) activity and mediates p53-dependent and independent cell ... The p53 tumor suppressor gene (TP53) is the most frequently altered gene in human cancer and also is found mutated in human ...
  http://jpet.aspetjournals.org/content/289/3/1306.long
*  Plus it
2003) The product of the Nf2 tumor suppressor gene, is an inhibitor of the p21-activated kinase, Pak1. Mol Cell 12:841-849. ... To elevate cAMP levels in cultured SCs, forskolin (5 μm; Sigma) was added into serum-free cell culture medium for 30 min. The ... 2008) Tumor suppressor schwannomin/merlin is critical for the organization of Schwann cell contacts in peripheral nerves. J ... 2008) Protein kinase A-mediated phosphorylation of the NF2 tumor suppressor protein merlin at serine 10 affects the actin ...
  http://www.jneurosci.org/content/32/48/17251
*  Autocrine Platelet-Derived Growth Factor-Dependent Gene Expression in Glioblastoma Cells Is Mediated Largely by Activation of...
... of the tumor suppressor p16/CDKN2A gene, amplification of the epidermal growth factor receptor (EGFR) gene, and mutations ... This pattern of autocrine PDGF-dependent gene expression in cultured cells also has been proven to be a reliable data set for ... such as the tumor-amplified kinase BTAK ( 43), MAPKKK5 (ASK1), IP3 kinase, Pak1, Sprouty 2, and SAK. The latter was noteworthy ... were actually associated with tumor suppressor functions. FEZ1, a leucine zipper protein associated with tumor suppressor ...
  http://cancerres.aacrjournals.org/content/65/13/5523
*  p57KIP2 Expression and Loss of Heterozygosity during Immortal Conversion of Cultured Human Mammary Epithelial Cells | Cancer...
... is a candidate tumor suppressor gene. Genes Dev., 9: 650-662, 1995. ... We have uncovered a novel role for the cyclin-dependent kinase inhibitor, p57KIP2, during the immortalization of cultured human ... whereas most human tumor tissues and cells display telomerase activity, and most cultured human tumor cells display stabilized ... whereas tumor tissues can give rise to cell lines of indefinite life span. Additionally, normal human somatic cells in vitro ...
  http://cancerres.aacrjournals.org/content/59/20/5112
*  RB1CC1 elisa kit | Human RB1-inducible coiled-coil 1 ELISA Kit-NP 001077086.1
... and cell migration. This tumor suppressor also enhances retinoblastoma 1 gene expression in cancer cells. Alternative splicing ... Abundantly expressed in human musculoskeletal and cultured osteosarcoma cells. Expression was difficult to detect in immature ... Human RB1-inducible coiled-coil protein 1 (RB1CC1) ELISA kit; CC1; FIP200; 200 kDa FAK family kinase-interacting protein; Rb1- ... It is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. Plays a crucial role ...
  https://www.mybiosource.com/prods/ELISA-Kit/Human/RB1-inducible-coiled-coil-1/RB1CC1/datasheet.php?products_id=9337415
*  SMAD4 Loss Is Associated with Cetuximab Resistance and Induction of MAPK/JNK Activation in Head and Neck Cancer Cells |...
DPC4, a candidate tumor suppressor gene, is altered infrequently in head and neck squamous cell carcinoma. Cancer Res 1996;56: ... 4A). To examine the effect of JNK inhibition on cell viability, SCC61-SC and SCC61-SMAD4KD cells were cultured in the presence ... we targeted these kinases with small-molecule inhibitors in SMAD4 knockdown and control cell lines. Cells were serum starved ... 14, 15). This switch enables tumor cells to leverage the tumor-promoting effects of TGFβ in the tumor microenvironment to ...
  http://clincancerres.aacrjournals.org/content/23/17/5162
*  Browse our DAPK3 ELISA Kits
Cellular and mouse modeling studies show that DAPK3 is a tumor suppressor gene and is important in early development. Also, its ... Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. ... Depletion of ZIPK resulted in impairment of cultured VSMC migration and contraction. ... death-associated protein kinase 3-like , DAP kinase 3 , DAP-like kinase , MYPT1 kinase , ZIP kinase isoform , ZIP-kinase , dlk ...
  https://www.antibodies-online.com/abstract/Death-Associated+Protein+Kinase+3+
*  Ski regulates Hippo and TAZ signaling to suppress breast cancer progression | Science Signaling
By investigating the tumor suppressor side of Ski, Rashidian et al. found that Ski inhibited TAZ in breast cancer cells. In the ... Ski effectively reversed transformation and epithelial-to-mesenchyme transition in cultured breast cancer cells and metastasis ... and cancer stem cell expansion. Hippo signaling activates the the Lats family of kinases, which phosphorylate TAZ and YAP, ... preventing the activation of genes that promote cell proliferation and survival. Ski has both pro-oncogenic and anti-oncogenic ...
  http://stke.sciencemag.org/content/8/363/ra14
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... resulting in expression of mutated oncogenes and deletion of tumor suppressor genes. The phenotypic consequences of these ... Cell Biology, Biochemistry, Issue 30, ubiquitination, cultured cell, in vitro system, immunoprecipitation, immunoblotting, ... Receptor tyrosine kinases have been implicated in the development and progression of many cancers, including both leukemia and ... gene expression, cell proliferation, and cell survival in the neuroepithelium10-20. Proteomic analyses of human, rat, mouse, ...
  https://www.jove.com/visualize/abstract/22328928/bace1-inhibition-induces-specific-cerebrospinal-fluid-amyloid-pattern
*  Plus it
... especially in Schwann cells. Merlin is quite atypical for a tumor-suppressor gene because it localizes to the cell membrane for ... For instance, melanocytes cultured from café au lait macules were found to exhibit a somatic mutation in the NF1 cell, implying ... the NF1 gene encodes the protein neurofibromin, which is an inhibitor of the ras/mitogen-activated protein kinase pathway. This ... What Is the Normal Function of the NF1 Gene?. NF1 is a tumor-suppressor gene encoded by chromosome 17q11.2, extending 283 ...
  http://www.ajnr.org/content/34/12/2250
*  Protocols and Video Articles Authored by Lijun Liu
A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma' ... Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy', ' ... p53 is an essential tumor-suppressor gene and p53 mutations play a critical role in tumor occurrence and progression (e.g., ... These kinases were rapidly activated by ouabain as in cultured cells. In isolated guinea pig hearts, 1 microM ouabain caused ...
  https://www.jove.com/author/Lijun_Liu
*  Hyperactivation of P21ras and the Hematopoietic-Specific Rho Gtpase, Rac2, Cooperate to Alter the Proliferation of...
Genetic and biochemical evidence that haploinsufficiency of the Nf1 tumor suppressor gene modulates melanocyte and mast cell ... 1997) Role of phosphoinositide 3-OH kinase in cell transformation and control of the actin cytoskeleton by Ras. Cell. 89:457- ... cultured from Nf1+/− mice demonstrate increased proliferation relative to wild-type cells in response to stem cell factor (SCF ... Mutations in the NF1 tumor suppressor gene cause neurofibromatosis type I (NF1), a disease characterized by the formation of ...
  http://jem.rupress.org/content/194/1/57
*  Plus it
With the overexpression of EGFR and inactivation of the PTEN tumor suppressor gene, there is constitutive activation of AKT and ... However, there is no difference in the expression of pAKT or pS6 kinase in the different AKT-transfected cell lines. Thus, ... There is at least one other report indicating a biphasic effect of curcumin on cultured neural progenitor cells in which lower ... Curcumin treatment of HNSCC cell lines. Cell lines were plated in 24-well plates, with 10,000 cells per well, and allowed to ...
  http://clincancerres.aacrjournals.org/content/14/19/6228