Loading...
*  Publications - Institute for Bioengineering of Catalonia (IBEC)
Keywords: 2A strain 2457T, Escherichia-Coli, Salmonella-Enterica, Protein, DNA, Expression, Binding, HHA, Shigella, Plasmid ... The hallmark of prioniopathies is the presence of an abnormal conformational isoform (PrPsc) of the natural cellular prion ... In particular, mice lacking specific domains of the PrPc protein have revealed the contribution of these domains to ... Keywords: Cell-adhesion, Conformational-changes, Electron-microscopy, Protein adsorption, Fractal dimension, Integrin binding, ...
  http://www.ibecbarcelona.eu/for-researchers/publications/2009/
*  Kuru prions and sporadic Creutzfeldt-Jakob disease prions have equivalent transmission properties in transgenic and wild-type...
... propagate as either variant CJD-like or sporadic CJD-like prion strains in transgenic mice expressing human prion protein. EMBO ... to an abnormal isoform, designated PrPSc (1, 2). This transition appears to involve only conformational change rather than ... of inoculated mice becoming infected (15, 26) (SI Table 1). Affected vCJD-inoculated Tg152 mice propagate a novel prion strain ... 152 mice (129VV Tg152 mice), have been described in refs. 9, 15, 26, and 51. This line of transgenic mice was originally ...
  http://www.pnas.org/content/105/10/3885.long
*  PRNP - Wikipedia
The mechanism for conformational conversion to the scrapie isoform is speculated to be an elusive ligand-protein, but, so far, ... However, more recent strains have shown significant cognitive abnormalities. As the null mice age, a marked loss of Purkinje ... NMR shows copper binding results in a conformational change at the N-terminus. PrPSc is a conformational isoform of PrPC, but ... PRNP (PRioN Protein) is the human gene encoding for the major prion protein PrP (for prion protein), also known as CD230 ( ...
  https://en.wikipedia.org/wiki/PRNP
*  Cross-sequence transmission of sporadic Creutzfeldt-Jakob disease creates a new prion strain
of molecular strain typing, in which different isoforms. of the prion protein are proposed to correspond to. individual strains ... sporadic CJD-like prion strains in transgenic mice. expressing human prion protein. The EMBO Journal Vol. 21 No. 23 pp. 6358 ... sCJD-VV2 prions generates a new prion strain with. altered conformational properties and disease. phenotypes as p-dCJD prions. ... my opinion, to continue with this myth that the U.K. strain of BSE (one strain TSE in. cows), and the nv/v CJD (one strain TSE ...
  http://www.vegsource.com/talk/madcow/messages/1001524.html
*  UCD Dublin | Research | Biomolecular & Biomed Science
Strain-specified relative conformational stability of the scrapie prion protein'. Protein Science, 10 :854-863. [Details] ... Chimeric prion protein expression in cultured cells and transgenic mice'. Protein Science, 1 :986-997. [Details] ... Attempts to convert the cellular prion protein into the scrapie isoform in cell-free systems'. Journal of Virology, 66 :6155- ... Propagation of prion strains through specific conformers of the prion protein'. Journal of Virology, 71 :9032-9044. [Details] ...
  http://www.ucd.ie/research/people/biomolecularbiomedscience/professormikescott/
*  MYL2 - Wikipedia
... is a protein that in humans is encoded by the MYL2 gene. This cardiac ventricular RLC isoform is distinct from that expressed ... Transgenic mice overexpressing the human MLC-2v R58Q mutation, which is associated with FHC has been shown to lead to a ... suggesting that the conformational change induced by calcium binding to RLC is functionally important. Another mode of RLC ... both epicardial and endocardial myofiber tension development and recovery to control cardiac torsion and myofiber strain ...
  https://en.wikipedia.org/wiki/MYL2
*  IJMS | Free Full-Text | Molecular Pathology of Lewy Body Diseases | HTML
Finally, differential expression changes of isoforms corresponding to genes primarily involved in Lewy body formation point to ... The impairment of protein degradation pathways, including both the ubiquitin-proteasome system and the autophagy- ... A protein-chameleon: conformational plasticity of alpha-synuclein, a disordered protein involved in neurodegenerative disorders ... Maraganore, DM; Wilkes, K; Lesnick, TG; Strain, KJ; de Andrade, M; Rocca, WA; Bower, JH; Ahlskog, JE; Lincoln, S; Farrer, MJ. A ...
  http://www.mdpi.com/1422-0067/10/3/724/htm
*  E ????E ???? ?? E??E (1996)(E )(336 ) 38 | ?????????.
mice expressing chimenc human-mouse priori protein. Proc Nat1 Acad Scr USA. 91,993&9940.. 19 Brown, P , Gibbs, C J , Jr, ... accumulation of abnormal isoforms of the priori protein (PrP) and the deposi-. tion of PrP amyloid m the central nervous system ... mvolves a conformational change with a shift from a-helix to P-sheet structure. (3--5). Amyloid formation occurs to the highest ... strains of transmtsstble mmk encephalopathy in hamsters J Gen Vu-01 73,32%334. 45 Tatzelt, J , Zuo, J , Voellmy, R , Scott, M ...
  http://nanobukva.ru/b/franch/Baker_H_F___Ridley_R_M__Prion_Diseases_
*  Frontiers | Potential role of glutathione in evolution of thiol-based redox signaling sites in proteins | Pharmacology
In favourable protein contexts, a bistable redox switch may be formed. Because of glutaredoxins similarities to thioredoxin, ... A redox buffer, glutathione is conjugated to reactive cysteine of endogenous proteins, inducing conformational changes in the ... In favourable protein contexts, a bistable redox switch may be formed. Because of glutaredoxins similarities to thioredoxin, ... We searched for proteins where the CSD is present, absent and also found as a single cysteine in protein orthologs. Three ...
  https://www.frontiersin.org/articles/10.3389/fphar.2015.00001/full
*  PCSK9 - Wikipedia
... investigators at Rockefeller University and University of Texas Southwestern had discovered the same protein in mice, and had ... doi:10.1016/j.str.2007.04.004. PMID 17502100. Bottomley MJ, Cirillo A, Orsatti L, Ruggeri L, Fisher TS, Santoro JC, Cummings RT ... The conformational change causes LDLR to release its LDL ligand, and the receptor is recycled back to the plasma membrane. ... This gene produces two isoforms through alternative splicing. PCSK9 is a member of the peptidase S8 family. The solved ...
  https://en.wikipedia.org/wiki/PCSK9
*  Untangling the Tauopathy for Alzheimer's disease and parkinsonism | SpringerLink
Tau is a microtubule-associated protein that mainly localizes to the axon to stabilize axonal microtubule structure and ... Distinct tau prion strains propagate in cells and mice and define different tauopathies. Neuron. 2014;82(6):1271-88.PubMed ... Cleavage and conformational changes of tau protein follow phosphorylation during Alzheimer's disease. Int J Exp Pathol. 2008;89 ... Cloning and sequencing of the cDNA encoding an isoform of microtubule-associated protein tau containing four tandem repeats: ...
  https://link.springer.com/article/10.1186%2Fs12929-018-0457-x
*  US20030049249A1 - Agent - Google Patents
... in prion diseases is a conformational change in cellular prion protein whereby it is converted into the pathologic isoform PrP ... Based on their studies in PrP null mice, Collinge et al. (1994) concluded that prion protein is necessary for normal synaptic ... New variant' CJD (vCJD) of humans is a distinct strain type of CJD that is associated with a pattern of PrP glycoforms that are ... This provided direct evidence that the pathogenic PrP protein can be formed from specific protein-protein interactions between ...
  https://patents.google.com/patent/US20030049249
*  Canonical and alternative transcript expression of PAX6 and CXCR4 in pancreatic cancer
The two isoforms differ only in the furthest N-terminal region of the protein, and this difference may affect how the receptor ... The two human CXCR4 isoforms display different HIV receptor activities: Consequences for the emergence of X4 strains. J Immunol ... Singh S, Mishra R, Arango NA, Deng JM, Behringer RR and Saunders GF: Iris hypoplasia in mice that lack the alternatively ... Identification of a Pax paired domain recognition sequence and evidence for DNA-dependent conformational changes. J Biol Chem. ...
  https://spandidos-publications.com/ol/13/6/4027
*  Mitofusin-1 protein is a generally expressed mediator of mitochondrial fusion in mammalian cells | Journal of Cell Science
Without the critical G2 motif threonine residue, the Mfn1T109A protein may not undergo the dramatic conformational change upon ... Inoue, K., Nakada, K., Ogura, A., Isobe, K., Goto, Y., Nonaka, I. and Hayashi, J. I. (2000). Generation of mice with ... cultured cells transiently transfected with Mfn1 and Mfn2 did not uncover biochemical interaction between these two isoforms. ... Human protein extracts were purchased from Clontech (Protein Medley Kidney and Heart). For mitochondrial protein extracts and ...
  http://jcs.biologists.org/content/116/13/2763?ijkey=3b32da1f5c4fb043ea4fee0a855567c11e6f797c&keytype2=tf_ipsecsha
*  CYP3A4 - Wikipedia
Like all members of this family, it is a hemoprotein, i.e. a protein containing a heme group with an iron atom. In humans, the ... Although humanized hCYP3A4 mice successfully expressed the enzyme in their intestinal tract, low levels of hCYP3A4 were found ... 1] Potent inhibition of human cytochrome P450 3A isoforms by cannabidiol: Role of phenolic hydroxyl groups in the resorcinol ... Shahrokh K, Cheatham TE, Yost GS (October 2012). "Conformational dynamics of CYP3A4 demonstrate the important role of Arg212 ...
  https://en.wikipedia.org/wiki/CYP3A4
*  Publications - Institute for Bioengineering of Catalonia (IBEC)
Keywords: QCM, Human plasma fibronectin, Induced conformational-changes, Von-willebrand-factor, BSA, Protein adsortion, Polymer ... Tg20 strain). By correlating histopathology with electrophysiology in living behaving mice, we demonstrate that both Prnp 2/2 ... The hallmark of prioniopathies is the presence of an abnormal conformational isoform (PrPsc) of the natural cellular prion ... This conductance switching may pose limitations to miniaturizing redox protein-based devices. Keywords: Bioelectronics, Protein ...
  http://www.ibecbarcelona.eu/for-researchers/publications/TOR/
*  Molecular and cellular neurobiotechnology - Institute for Bioengineering of Catalonia (IBEC)
... or that lack prion protein (knock-out) to generate mice that express varying amounts of prion protein and deposit β-amyloid. ... The hallmark of prioniopathies is the presence of an abnormal conformational isoform (PrPsc) of the natural cellular prion ... Understanding the structural basis for strains and the basis for adaptation of a strain to a new host will require continued ... We observed FoxP2 protein expressed in a gradient from the subventricular zone to the mantle layer in mice embryos. In the ...
  http://ibecbarcelona.eu/ca/recerca/molecular-and-cellular-neurobiotechnology/
*  Evaluation of an active immunoprophylaxis against prion diseases - PDF
... protein According to the protein only hypothesis postulated by Stanley Prusiner the prion protein in its pathogenic isoform PrP ... 18 INTRODUCTION destabilize the prion protein and might lead to spontaneous conformational changes (Owen et al., 1989). Figure ... Most animals immunized did not show a prolongation of incubation time compared to untreated control mice. Three mice treated ... prion protein Features of PrP Physiological function of PrP Conformation of PrP C and PrP Sc Species barrier and prion strains ...
  http://docplayer.net/1422132-Evaluation-of-an-active-immunoprophylaxis-against-prion-diseases.html
*  HUMAN and ANIMAL TSE Classifications i.e. mad cow disease and the UKBSEnvCJD only theory
of molecular strain typing, in which different isoforms. of the prion protein are proposed to correspond to. individual strains ... protein (PrPSc) in inoculated mice.. Reliable comparisons of mouse prion strain properties. can only be achieved after passage ... means by which such conformational difference is detected. is somewhat indirect; relying on the action of proteases,. primarily ... sporadic CJD-like prion strains in transgenic mice. expressing human prion protein. The EMBO Journal Vol. 21 No. 23 pp. 6358 ...
  http://vegsource.com/talk/madcow/messages/1000420.html
*  http://ufdc.ufl.edu/UFE0042392/00001
... s uch as mice fed a high iron diet or Hfe knockout mice (61) On the other hand, TfR2 protein was down regulated in ... Multiple isoforms of DMT1 transcripts have been identified with two isoforms (67) On ly the IRE form of DMT1 is PAGE 39. 39 ... The acidic pH of the endosomal lumen induces a conformational change in Tf that results in iron release. The free Fe 3+ ... Another group also identified a mutation in DMT1 in an inbred mouse strain ( mk ) with severe microcytic an emia (64) Later, ...
  http://ufdc.ufl.edu/UFE0042392/00001
*  Proteasome - Wikipedia
Wenzel T, Baumeister W (Mar 1995). "Conformational constraints in protein degradation by the 20S proteasome". Nature Structural ... doi:10.1016/j.str.2006.05.019. PMID 16843899. Krüger E, Kloetzel PM, Enenkel C (2001). "20S proteasome biogenesis". Biochimie. ... For example, studies in mice bearing human skin grafts found a reduction in the size of lesions from psoriasis after treatment ... Although the precise function of these proteasome isoforms is still largely unknown, cells expressing these proteasomes show ...
  https://en.wikipedia.org/wiki/Proteasome
*  Frontiers | Regulation of Ras Exchange Factors and Cellular Localization of Ras Activation by Lipid Messengers in T Cells |...
The calcium-binding event induces protein conformational changes through the alteration of the directional vectors of the α- ... In agreement with a PLCγ1-DAG-RasGRP1 signaling axis (Figure 5), conditional PLCγ1 knockout mice and RasGRP1-deficient mice ... Localization of the src gene product of the Harvey strain of MSV to plasma membrane of transformed cells by electron ... An acylation cycle regulates localization and activity of palmitoylated Ras isoforms. Science (2005) 307:1746-52. doi:10.1126/ ...
  https://www.frontiersin.org/articles/10.3389/fimmu.2013.00239/full
*  Frontiers | The Molecular Mechanism of Natural Killer Cells Function and Its Importance in Cancer Immunotherapy | Immunology
In mice, NKG2D is present in two different isoforms, short isoform (NKG2D-S) and a long isoform (NKG2D-L), which are generated ... of death receptor with its cognate ligand induces a conformational change in the receptor and recruitment of adaptor protein ... NK cells are identified as CD3−NK1.1+ cells in C57BL/6, FVB/N, and NZB strains of mice. BALB/c, CBA/J, AKR, C3H, DBA/1, DBA/2, ... Adoptive transfer of bone marrow cells from Rae-1ε transgenic mice to syngeneic wild-type mice leads to efficient rejection of ...
  https://www.frontiersin.org/articles/10.3389/fimmu.2017.01124/full
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Certain strains of genetically modified mice, such as Atm-/- and p53-/- consistently develop thymic lymphoma early in life 1,2 ... but it also enhances our understanding of the protein folding process and protein-protein interactions. Experimental methods ... Notably, the C-terminal domain PHD of RBP2 is absent in the smaller RBP2 isoform4. It is conceivable that the small isoform of ... This may be due, in part, to the lack of chiral centers and conformational constraints in peptoid molecules. Recently, we ...
  https://www.jove.com/visualize/abstract/24260260/identification-highly-methylated-genes-across-various-types-b-cell
*  CREUTZFELDT JAKOB DISEASE: March 2013
... and consist primarily of scrapie prion protein (PrPSc), a pathogenic isoform of the host-encoded cellular prion protein (PrPC ... Mounting evidence suggests that prion-strain-specific features segregate with different PrPSc conformational and aggregation ... presence of this prion strain at the first passage or the persistence of both types of PrPSc through serial passages in mice.98 ... and consist primarily of scrapie prion protein (PrPSc), a pathogenic isoform of the host-encoded cellular prion protein (PrPC ...
  http://creutzfeldt-jakob-disease.blogspot.com/2013_03_01_archive.html