Cross-sequence transmission of sporadic Creutzfeldt-Jakob disease creates a new prion strain
of molecular strain typing, in which different isoforms. of the prion protein are proposed to correspond to. individual strains ... sporadic CJD-like prion strains in transgenic mice. expressing human prion protein. The EMBO Journal Vol. 21 No. 23 pp. 6358 ... sCJD-VV2 prions generates a new prion strain with. altered conformational properties and disease. phenotypes as p-dCJD prions. ... my opinion, to continue with this myth that the U.K. strain of BSE (one strain TSE in. cows), and the nv/v CJD (one strain TSE ...http://www.vegsource.com/talk/madcow/messages/1001524.html
UCD Dublin | Research | Biomolecular & Biomed Science
Strain-specified relative conformational stability of the scrapie prion protein'. Protein Science, 10 :854-863. [Details] ... Chimeric prion protein expression in cultured cells and transgenic mice'. Protein Science, 1 :986-997. [Details] ... Attempts to convert the cellular prion protein into the scrapie isoform in cell-free systems'. Journal of Virology, 66 :6155- ... Propagation of prion strains through specific conformers of the prion protein'. Journal of Virology, 71 :9032-9044. [Details] ...http://www.ucd.ie/research/people/biomolecularbiomedscience/professormikescott/
PRNP - Wikipedia
The mechanism for conformational conversion to the scrapie isoform is speculated to be an elusive ligand-protein, but, so far, ... However, more recent strains have shown significant cognitive abnormalities. As the null mice age, a marked loss of Purkinje ... NMR shows copper binding results in a conformational change at the N-terminus. PrPSc is a conformational isoform of PrPC, but ... PRNP (PRioN Protein) is the human gene encoding for the major prion protein PrP (for prion protein), also known as CD230 ( ...https://en.wikipedia.org/wiki/PRNP
IJMS | Free Full-Text | Molecular Pathology of Lewy Body Diseases | HTML
Finally, differential expression changes of isoforms corresponding to genes primarily involved in Lewy body formation point to ... The impairment of protein degradation pathways, including both the ubiquitin-proteasome system and the autophagy- ... A protein-chameleon: conformational plasticity of alpha-synuclein, a disordered protein involved in neurodegenerative disorders ... Maraganore, DM; Wilkes, K; Lesnick, TG; Strain, KJ; de Andrade, M; Rocca, WA; Bower, JH; Ahlskog, JE; Lincoln, S; Farrer, MJ. A ...http://www.mdpi.com/1422-0067/10/3/724/htm
E ????E ???? ?? E??E (1996)(E )(336 ) 38 | ?????????.
mice expressing chimenc human-mouse priori protein. Proc Nat1 Acad Scr USA. 91,993&9940.. 19 Brown, P , Gibbs, C J , Jr, ... accumulation of abnormal isoforms of the priori protein (PrP) and the deposi-. tion of PrP amyloid m the central nervous system ... mvolves a conformational change with a shift from a-helix to P-sheet structure. (3--5). Amyloid formation occurs to the highest ... strains of transmtsstble mmk encephalopathy in hamsters J Gen Vu-01 73,32%334. 45 Tatzelt, J , Zuo, J , Voellmy, R , Scott, M ...http://nanobukva.ru/b/franch/Baker_H_F___Ridley_R_M__Prion_Diseases_
MYL2 - Wikipedia
... is a protein that in humans is encoded by the MYL2 gene. This cardiac ventricular RLC isoform is distinct from that expressed ... Transgenic mice overexpressing the human MLC-2v R58Q mutation, which is associated with FHC has been shown to lead to a ... suggesting that the conformational change induced by calcium binding to RLC is functionally important. Another mode of RLC ... both epicardial and endocardial myofiber tension development and recovery to control cardiac torsion and myofiber strain ...https://en.wikipedia.org/wiki/MYL2
US20030049249A1 - Agent - Google Patents
... in prion diseases is a conformational change in cellular prion protein whereby it is converted into the pathologic isoform PrP ... Based on their studies in PrP null mice, Collinge et al. (1994) concluded that prion protein is necessary for normal synaptic ... New variant' CJD (vCJD) of humans is a distinct strain type of CJD that is associated with a pattern of PrP glycoforms that are ... This provided direct evidence that the pathogenic PrP protein can be formed from specific protein-protein interactions between ...https://patents.google.com/patent/US20030049249
Frontiers | Potential role of glutathione in evolution of thiol-based redox signaling sites in proteins | Pharmacology
In favourable protein contexts, a bistable redox switch may be formed. Because of glutaredoxins similarities to thioredoxin, ... A redox buffer, glutathione is conjugated to reactive cysteine of endogenous proteins, inducing conformational changes in the ... In favourable protein contexts, a bistable redox switch may be formed. Because of glutaredoxins similarities to thioredoxin, ... We searched for proteins where the CSD is present, absent and also found as a single cysteine in protein orthologs. Three ...https://www.frontiersin.org/articles/10.3389/fphar.2015.00001/full
HUMAN and ANIMAL TSE Classifications i.e. mad cow disease and the UKBSEnvCJD only theory
of molecular strain typing, in which different isoforms. of the prion protein are proposed to correspond to. individual strains ... protein (PrPSc) in inoculated mice.. Reliable comparisons of mouse prion strain properties. can only be achieved after passage ... means by which such conformational difference is detected. is somewhat indirect; relying on the action of proteases,. primarily ... sporadic CJD-like prion strains in transgenic mice. expressing human prion protein. The EMBO Journal Vol. 21 No. 23 pp. 6358 ...http://vegsource.com/talk/madcow/messages/1000420.html
Canonical and alternative transcript expression of PAX6 and CXCR4 in pancreatic cancer
The two isoforms differ only in the furthest N-terminal region of the protein, and this difference may affect how the receptor ... The two human CXCR4 isoforms display different HIV receptor activities: Consequences for the emergence of X4 strains. J Immunol ... Singh S, Mishra R, Arango NA, Deng JM, Behringer RR and Saunders GF: Iris hypoplasia in mice that lack the alternatively ... Identification of a Pax paired domain recognition sequence and evidence for DNA-dependent conformational changes. J Biol Chem. ...https://spandidos-publications.com/ol/13/6/4027
Mitofusin-1 protein is a generally expressed mediator of mitochondrial fusion in mammalian cells | Journal of Cell Science
Without the critical G2 motif threonine residue, the Mfn1T109A protein may not undergo the dramatic conformational change upon ... Inoue, K., Nakada, K., Ogura, A., Isobe, K., Goto, Y., Nonaka, I. and Hayashi, J. I. (2000). Generation of mice with ... cultured cells transiently transfected with Mfn1 and Mfn2 did not uncover biochemical interaction between these two isoforms. ... Human protein extracts were purchased from Clontech (Protein Medley Kidney and Heart). For mitochondrial protein extracts and ...http://jcs.biologists.org/content/116/13/2763?ijkey=3b32da1f5c4fb043ea4fee0a855567c11e6f797c&keytype2=tf_ipsecsha
The origin of the prion agent of kuru: molecular and biological strain typing | Philosophical Transactions of the Royal Society...
1995 Unaltered susceptibility to BSE in transgenic mice expressing human prion protein. Nature. 378, 779-783. doi:10.1038/ ... 1996 Evidence for the conformation of the pathologic isoform of the prion protein enciphering and propagating prion diversity. ... These data are consistent with a conformational selection model of prion transmission barriers (Collinge 1999, 2001; Hill & ... 152 mice (129VV Tg152 mice; Collinge et al. 1995), and into wild-type FVB/NHsd mice (genotype Prnpa). The number of prion ...http://rstb.royalsocietypublishing.org/content/363/1510/3747
... s uch as mice fed a high iron diet or Hfe knockout mice (61) On the other hand, TfR2 protein was down regulated in ... Multiple isoforms of DMT1 transcripts have been identified with two isoforms (67) On ly the IRE form of DMT1 is PAGE 39. 39 ... The acidic pH of the endosomal lumen induces a conformational change in Tf that results in iron release. The free Fe 3+ ... Another group also identified a mutation in DMT1 in an inbred mouse strain ( mk ) with severe microcytic an emia (64) Later, ...http://ufdc.ufl.edu/UFE0042392/00001
Frontiers | The Molecular Mechanism of Natural Killer Cells Function and Its Importance in Cancer Immunotherapy | Immunology
In mice, NKG2D is present in two different isoforms, short isoform (NKG2D-S) and a long isoform (NKG2D-L), which are generated ... of death receptor with its cognate ligand induces a conformational change in the receptor and recruitment of adaptor protein ... NK cells are identified as CD3−NK1.1+ cells in C57BL/6, FVB/N, and NZB strains of mice. BALB/c, CBA/J, AKR, C3H, DBA/1, DBA/2, ... Adoptive transfer of bone marrow cells from Rae-1ε transgenic mice to syngeneic wild-type mice leads to efficient rejection of ...https://www.frontiersin.org/articles/10.3389/fimmu.2017.01124/full
Patent US6303652 - BTK inhibitors and methods for their identification and use - Google Patents
Isoform of bruton's tyrosine kinase (BTK) protein. US8889643. Jun 25, 2012. Nov 18, 2014. Bionsil, S.r.l.. Isoform of bruton's ... 1993) Nature 361, 226-233). In mice, mutations in the BTK gene have been identified as the cause of murine X-linked immune ... The generated recombinant plasmids were transformed into the E. coli strain DH5a. Single transformants were expanded in 5 ml ... This conformational similarity with the crystal structures indicated that the molecular models used for docking were ...http://www.google.com/patents/US6303652?dq=inassignee:doubleclick
PCSK9 - Wikipedia
... investigators at Rockefeller University and University of Texas Southwestern had discovered the same protein in mice, and had ... doi:10.1016/j.str.2007.04.004. PMID 17502100. Bottomley MJ, Cirillo A, Orsatti L, Ruggeri L, Fisher TS, Santoro JC, Cummings RT ... The conformational change causes LDLR to release its LDL ligand, and the receptor is recycled back to the plasma membrane. ... This gene produces two isoforms through alternative splicing. PCSK9 is a member of the peptidase S8 family. The solved ...https://en.wikipedia.org/wiki/PCSK9
Molecular and cellular neurobiotechnology - Institute for Bioengineering of Catalonia (IBEC)
... or that lack prion protein (knock-out) to generate mice that express varying amounts of prion protein and deposit Î²-amyloid. ... The hallmark of prioniopathies is the presence of an abnormal conformational isoform (PrPsc) of the natural cellular prion ... Understanding the structural basis for strains and the basis for adaptation of a strain to a new host will require continued ... We observed FoxP2 protein expressed in a gradient from the subventricular zone to the mantle layer in mice embryos. In the ...http://ibecbarcelona.eu/ca/recerca/molecular-and-cellular-neurobiotechnology/
The mitochondrial protein Bak is pivotal for gliotoxin-induced apoptosis and a critical host factor of Aspergillus fumigatus...
... mice subsequently infected with a GT-producing A. fumigatus strain. The presence of GT synthesis by this fungal strain was ... Cell damage-induced conformational changes of the pro-apoptotic protein Bak in vivo precede the onset of apoptosis. J. Cell ... Mouse VDAC isoforms expressed in yeast: channel properties and their roles in mitochondrial outer membrane permeability. J. ... mice are resistant to A. fumigatus infection. Female wt C57BL6 or Bak−/− mice (6 mice per group) were immunosuppressed by ...http://jcb.rupress.org/content/174/4/509
Pleiotropy of Glycogen Synthase Kinase-3 Inhibition by CHIR99021 Promotes Self-Renewal of Embryonic Stem Cells from Refractory...
... and BALB/c mouse strains through stabilization of β-catenin and c-Myc protein levels. Stabilized β-catenin promoted ES self- ... β-catenin and c-Myc have similar effects on ES cells derived from both B6 and BALB/c mice. Conclusions/Significance Our data ... Further, elevated c-Myc protein levels did not contribute significantly to CH-mediated ES cell self-renewal. Instead, the role ... Here, we dissect the mechanism of GSK-3 inhibition by CHIR99021 in mouse ES cells from refractory mouse strains. Methodology/ ...http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0035892
Cellular prion protein (PrPC) protects neuronal cells from the effect of huntingtin aggregation | Journal of Cell Science
Colling, S. B., Collinge, J. and Jefferys, J. G. R. (1996). Hippocampal slices from prion protein null mice: disrupted Ca2+- ... Cellular isoform of the prion protein PrPC in human intestinal cell lines: Genetic polymorphism at codon 129, mRNA ... Cellular prion protein inhibits proapoptotic Bax conformational change in human neurons and in breast carcinoma MCF-7 cells. ... The chronically infected ScSN56 cell line infected with the Chandler strain of scrapie was a generous gift from Byron Caughey ( ...http://jcs.biologists.org/content/120/15/2663
Genetic variation and exercise-induced muscle damage: implications for athletic performance, injury and ageing |...
Role of TNF receptor 1 in protein turnover during cancer cachexia using gene knockout mice. Mol Cell Endocrinol 142(1-2):183- ... Gautel M (2011) The sarcomeric cytoskeleton: who picks up the strain? Curr Opin Cell Biol 23(1):39-46 PubMedCrossRef ... Wilson IA, Brindle KM, Fulton A (1995) Differential localization of the mRNA of the M and B isoforms of creatine kinase in ... A predictive evidence for conformational change to influence mitochondrial transport and a study of allelic association in ...https://www.springermedizin.de/genetic-variation-and-exercise-induced-muscle-damage-implication/10239762
Evaluation of an active immunoprophylaxis against prion diseases - PDF
... protein According to the protein only hypothesis postulated by Stanley Prusiner the prion protein in its pathogenic isoform PrP ... 18 INTRODUCTION destabilize the prion protein and might lead to spontaneous conformational changes (Owen et al., 1989). Figure ... Most animals immunized did not show a prolongation of incubation time compared to untreated control mice. Three mice treated ... prion protein Features of PrP Physiological function of PrP Conformation of PrP C and PrP Sc Species barrier and prion strains ...http://docplayer.net/1422132-Evaluation-of-an-active-immunoprophylaxis-against-prion-diseases.html
Cutting Edge: Brucella abortus Exploits a Cellular Prion Protein on Intestinal M Cells as an Invasive Receptor | The Journal of...
Prnp−/− mice (14) were backcrossed onto a C57BL/6 background. Other PrPC-deficient (Prnp−/−) mice (RBRC00437) were provided by ... Antigenic characterization of an abnormal isoform of prion protein using a new diverse panel of monoclonal antibodies. Virology ... because phagosomes containing a virB mutant strain of B. abortus fuse with lysosomes to form conventional phagolysosomes (12). ... replicates by interacting with cellular PrPC and mediating its conformational change into the disease-causing PrPSc (19). ...http://www.jimmunol.org/content/189/4/1540
CYP3A4 - Wikipedia
Like all members of this family, it is a hemoprotein, i.e. a protein containing a heme group with an iron atom. In humans, the ... Although humanized hCYP3A4 mice successfully expressed the enzyme in their intestinal tract, low levels of hCYP3A4 were found ... 1] Potent inhibition of human cytochrome P450 3A isoforms by cannabidiol: Role of phenolic hydroxyl groups in the resorcinol ... Shahrokh K, Cheatham TE, Yost GS (October 2012). "Conformational dynamics of CYP3A4 demonstrate the important role of Arg212 ...https://en.wikipedia.org/wiki/CYP3A4
... protein) for 2 weeks before randomization and treatment. Mice were treated with vehicle, FGF21 protein at 1 mg/kg daily, or CVX ... The antibody does not bind other isoforms of FGFR or βKlotho, but rather induces its effects by homodimerization and activation ... The bacterial expression vectors were transformed into the host strain BL21-(DE3)-RIL (Stratagene). The transformed cells were ... sites critical for bioactivity attributable to either steric hindrance afforded by the scaffold antibody and/or conformational ...http://jpet.aspetjournals.org/content/346/2/270