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*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Interaction of HA with its cell surface receptor CD44 induces signaling events that promote tumor cell growth, survival, and ... We have recently developed a 3D in vitro system to model the in vivo growth characteristics of cells that exhibit either ... Tumor Cells, Cultured, Early Detection of Cancer, senescence, cancer, cells, flow cytometry, C12FDG, cell culture, clinical ... We found that some tumor cell lines such as melanoma B16F1 cells, glioblastoma U87 cells, and breast cancer MDA-MB-435 cells ...
  https://www.jove.com/visualize/abstract/23028492/salinomycin-induces-autophagy-colon-breast-cancer-cells-with
*  Patent US6087157 - Device and method for analyzing tumor cell invasion of an extracellular matrix - Google Patents
Methods for culturing eukaryotic cells and studying their growth characteristics, including the invasive growth characteristics ... of tumor cells, on the submucosal matrix are described. ... A device for analyzing the in-vitro growth properties of cells ... inexpensive cell culture growth substrate that promotes or induces growth and differentiation of cells cultured in vitro. ... the cell/matrix interactions are different when the cells are cultured on the abluminal versus the luminal side of the ...
  http://www.google.com/patents/US6087157?dq=6,232,546
*  Herpes Simplex Virus 1 Infection Promotes the Growth of a Subpopulation of Tumor Cells in Three-Dimensional Uveal Melanoma...
Several aspects of HSV-1-tumor cell interactions have been difficult to study in vitro, as traditional two-dimensional (2D) ... infection in some tumor cells within the matrix and this persistent infection induces increased growth of infected cells. In ... as it destroyed the cultured cells through lytic infection, also suggesting that noncytolytic HSV infections could have a ... K26GFP was shown to grow with wild-type virus characteristics in cell culture (25). ...
  https://jvi.asm.org/content/92/19/e00700-18
*  SMART: SERPIN domain annotation
... that induces a neuronal phenotype in cultured human retinoblastoma cells. Morphological changes include the induction of an ... Transfection of MDA-MB-435 mammary carcinoma cells with the maspin gene did not alter the cells' growth properties in vitro, ... Maspin, a serpin with tumor-suppressing activity in human mammary epithelial cells. ... Since CrmA possesses characteristics shared by inhibitors of serine proteinases, we presume that ICE, though it is a cysteine ...
  http://smart.embl-heidelberg.de/smart/do_annotation.pl?DOMAIN=SERPIN&BLAST=DUMMY
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... imaging of T-cell interaction with tumor cells and tumor cells migration along the collagen IV of basement membrane. Taken ... We have recently developed a 3D in vitro system to model the in vivo growth characteristics of cells that exhibit either ... and cultured with IL-7 + IL-15 for additional 4 days. Tumor-specificity and anti-tumor efficacy of the ex vivo expanded T cells ... and can distinguish between metastatic and non-metastatic tumor clones; and 3) curative tumor resection induces dynamic changes ...
  https://www.jove.com/visualize/abstract/19557135/a-systems-immunology-approach-to-host-tumor-interaction-large-scale
*  Protocols and Video Articles Authored by Huey-Kang Sytwu (Translated to Swedish)
... secreted factors on auditory cell proliferation in vitro. In the present work, two auditory cell types were cultured in the ... Triptolide not only inhibits tumor growth but also induces apoptosis of these drug-resistant cancer cells in xenograft mouse ... Daxx and TCF4 Interaction Links to Oral Squamous Cell Carcinoma Growth by Promoting Cell Cycle Progression Via Induction of ... Mesenchymal Stem Cells from Rat Olfactory Bulbs Can Differentiate into Cells with Cardiomyocyte Characteristics Journal of ...
  https://www.jove.com/author/Huey-Kang_Sytwu?language=Swedish
*  Lack of transforming growth factor-β signaling promotes collective cancer cell invasion through tumor-stromal crosstalk |...
We hypothesize that TGF-β is a critical regulator of tumor-stromal interactions that promote mammary tumor cell migration and ... Immunoblotting and quantitative PCR analyses on cultured cells validated these targets and correlated Tmeff1 expression with ... Fibroblast-stimulated carcinoma cells utilize TGF-β signaling to drive single cell/strand migration but migrate collectively in ... At epithelial-stromal boundaries, single cell/strand migration of TβRIIfl/fl carcinoma cells was characterized by expression of ...
  https://breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3217
*  Role of Recombinant DNA Technology to Improve Life
Highly sustained levels of cells that were engineered for tumor recognition in blood using a retrovirus encoding a T-cell ... A higher level of transgenic expression was mediated and an enhanced antitumor effect was observed on in vitro HCC cells while ... Vial-mediated delivery of the CRISPR/Cas9 system to somatic cells of adult animals induces specific chromosomal rearrangements ... Testosterone production by stimulated hCG can be inhibited by each of these antibodies in cells cultured by LEYDIG and uterine ...
  https://www.hindawi.com/journals/ijg/2016/2405954/
*  DNA- methylation changes in a human cell model of breast cancer progression
... the tumor cell lines, caMCFs, which shown all characteristics of a fully malignant breast cancer cell types [6] (Fig.1). The ... All the cells from the in vitro model showed low SPRY1 increases after treatment with 5-aza-dC (2.32-fold in MCF-10F, 2.6- fold ... 17-Beta-estradiol induces transformation and tumorigenesis in human breast epithelial cells. FASEB J. 2006;20:1622-1634. [ ... The cellular response to neuregulins is governed by complex interactions of the erbB receptor family. Mol Cell Biol. 1995;15: ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2872054/?lang=en-ca
*  Gr-1+ Myeloid Cells Derived from Tumor-Bearing Mice Inhibit Primary T Cell Activation Induced Through CD3/CD28 Costimulation |...
It is known that costimulation through B7-CD28 interaction in the presence of TCR signaling induces full T cell activation. ... natural suppressor cells, T cells become deactivated or tolerized. This deactivation of T cells, at least in vitro, can be ... as responder cells. Irradiated stimulator cells (5 × 105/well) and responder cells (2 × 105/well) were cultured in 96-well ... To enrich myeloid cell populations according to their density characteristics, splenic or BM cell suspensions were depleted of ...
  http://www.jimmunol.org/content/165/2/779?ijkey=bd3bebf684d09b9db814aae0078a2ec38a2c39b8&keytype2=tf_ipsecsha
*  Developing and applying a gene functional association network for anti-angiogenic kinase inhibitor activity assessment in an...
... in which human umbilical vein endothelial cells (HUVECs) formed capillary structures when co-cultured with normal human dermal ... The interactome includes the involvement of oncogenes and tumor suppressor genes in angiogenesis. We developed a network ... We established a comprehensive gene functional association network to model in vitro angiogenesis regulation. The present study ... the resulting interactome captured 17226 functional gene associations and displayed characteristics of a scale-free network. ...
  https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-9-264
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
To test for an interaction between two proteins of interest in cultured mammalian cells, one protein is expressed as a fusion ... and number of tumor spheres formed represents CSC activity harbored in either bulk populations of cultured cancer cells or ... Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies. Authors: Enza Lonardo, Michele ... here we describe in vitro screening methods that utilizes the mouse BAF3 cells expressing the random mutation library of JAK2 ...
  https://www.jove.com/visualize/abstract/26087013/clonal-evolution-blast-crisis-correlate-with-enhanced-proteolytic
*  Protocols and Video Articles Authored by Yi Pan (Translated to Italian)
Many research studies have shown that insulin-producing cells can be derived from the in-vitro cultured pancreatic colonies ... We found that PD-L1 was present both on the surface of tumor cells and tumor-infiltrating immune cells. Patients with tumor- ... Previous studies were mostly focused on either bacteria itself or host cells, the interaction mechanism of host-pathogen awaits ... required for cell growth and proliferation. On the other hand, deprivation of amino acids induces autophagy via inhibition of ...
  https://www.jove.com/author/Yi_Pan?language=Italian
*  Induction of Immunogenic Tumor Cell Death by Attenuated Oncolytic Measles Virus | OMICS International
... namely replicative viruses that exclusively or preferentially infect and kill tumor.. ... They also showed that DC co-cultured with MV-infected melanoma tumor cells induces cytotoxic T-cell responses against tumor ... Interaction of MV infected tumor cells and dendritic cells. Our laboratory and others have been interested in characterizing, ... in vitro, how MV-infected tumor cells stimulate APC such as DC to induce their capacities to stimulate an adaptive antitumor ...
  https://www.omicsonline.org/open-access/induction-of-immunogenic-tumor-cell-death-by-attenuated-oncolytic-measles-virus-2155-9899-1000291.php?aid=38192
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Tumor Cells, Cultured, Early Detection of Cancer, senescence, cancer, cells, flow cytometry, C12FDG, cell culture, clinical ... This report describes protocols to monitor autophagy-cytoskeleton interactions caused by S. flexneri in vitro using tissue ... High glucose induces bone marrow-derived mesenchymal stem cell senescence by upregulating autophagy. ... as a cellular scaffold and is an efficient culture technique because the cells respond to the topographic characteristics of ...
  https://www.jove.com/visualize/abstract/25961745/high-glucose-induces-bone-marrow-derived-mesenchymal-stem-cell
*  T Helper 1 (Th1) and Th2 Characteristics Start to Develop During T Cell Priming and Are Associated with an Immediate Ability to...
1 or Swiss type mouse mammary tumor virus [MMTV(SW)] ((26)). MMTV(SW) induces both a polyclonal and envelope-specific ... The antibody-producing cells arise from cognate T cell B cell interaction, revealed by the respective induction of Cγ2a and Cγ1 ... This correlates with findings from studies in vitro, where signals from T cells like CD40 ligation on B cells can induce Ig ... 45)) reported that naïve T cells cultured with Con A initially upregulate both IFN-γ and IL-4 message, but when IL-4 is present ...
  http://jem.rupress.org/content/187/8/1193
*  Cancer Research
Transplantation of iPS-Derived Tumor Cells with a Homozygous MHC Haplotype Induces GRP94 Antibody Production in MHC-Matched ... In this study, we demonstrate that autoantibodies can mitigate tumor growth in vitro and in vivo. We generated two cancer cell ... to comprehensively investigate molecular characterization of tumor-immune interactions. Levels of six tumor-infiltrating immune ... Metastatic cells cultured on osteo-mimetic surfaces coated with tenascin C exhibited enhanced adhesion and colony formation as ...
  https://medworm.com/journal/cancer-research/
*  Modulated Gene Expression in 3D Cell Culture | Sigma-Aldrich
Introduction of the modulation in expression profile for some genes and proteins when comparing 2D versus 3D cell culture ... Tensile strain induces integrin beta1 and ILK expression higher and faster in 3D cultured rat skeletal myoblasts than in 2D ... Ovarian normal and tumor-associated fibroblasts retain in vivo stromal characteristics in a 3-D matrix-dependent manner. ... Use of a three-dimensional cell culture model to study airway smooth muscle-mast cell interactions in airway remodeling. Am. J ...
  https://www.sigmaaldrich.com/technical-documents/articles/biology/modulated-gene-expression-3d-cell-culture.html
*  Autophagy as a Therapeutic Target in Multiple Diseases: From Molecular Mechanisms to Drug Discovery | The New York Academy of...
The regulation of autophagy in cancer cells can enhance tumor cell survival, yet can also suppress the initiation of tumor ... was a potent inducer of autophagy in cultured mammalian cells as well as in vivo in mice. We identified a cellular target of ... Levine is internationally recognized as a leading authority in the field of virus-host interactions and in the field of ... peptide decreased huntingtin protein aggregates in cells expressing mutant huntingtin and decreased the in vitro replication of ...
  https://www.nyas.org/events/2012/autophagy-as-a-therapeutic-target-in-multiple-diseases-from-molecular-mechanisms-to-drug-discovery/
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Applying a direct current electric field to cultured cells in vitro induces directional cell migration, or galvanotaxis. The 2- ... Melanoma cells exhibit the same characteristics reflecting the original tumor stage (RGP, VGP and metastatic melanoma cells) in ... skin reconstruct models has enabled the field to investigate cell-matrix and cell-cell interactions between different cell ... Tumor Cells, Cultured, Early Detection of Cancer, senescence, cancer, cells, flow cytometry, C12FDG, cell culture, clinical ...
  https://www.jove.com/visualize/abstract/20856861/mnsod-upregulation-induces-autophagic-programmed-cell-death-senescent
*  Dendritic cell - Wikipedia
... dendritic cells cultured in vitro do not show the same behaviour or capability as dendritic cells isolated ex vivo. Nonetheless ... concurrent interaction of all three cell types, namely CD4+ T helper cells, CD8+ T cells and dendritic cells, seems to be ... result in isolation of cells with characteristics unique to DCs. The dendritic cells are constantly in communication with other ... Subsequent treatment with tumor necrosis factor (TNF) further differentiates the iDCs into mature dendritic cells. Monocytes ...
  https://en.wikipedia.org/wiki/Dendritic_cell
*  Mucin 1 gene silencing inhibits the growth of SMMC-7721 human hepatoma cells through Bax-mediated mitochondrial and caspase-8...
... a clone formation assay in vitro and a tumor xenograft mouse model with an in vivo imaging system were utilized and the growth ... Cells were cultured for 48 h, 5×106 cells were harvested and the DNA was extracted using an Apoptotic DNA Ladder Extraction kit ... MUC1 gene silencing induces apoptotic phenotypic alterations in SMMC-7721 cells. (A) Detection of cell nuclear condensation by ... 8 and these interactions were reduced upon MUC1 gene silencing in SMMC‑7721 cells. The above results indicate that MUC1 gene ...
  https://spandidos-publications.com/mmr/12/5/6782
*  Expression of the miR200 Family of microRNAs in Mesothelial Cells Suppresses the Dissemination of Ovarian Cancer Cells |...
... once the integrity of the mesothelial cells is disrupted by tumor cells or oxidative stress, the mesothelial cells acquire the ... 1A). We next examined the proliferation of cells cultured on HPMCs incubated with or without TGFβ. ES-2 and SKOV3 cells that ... Our results define the novel interaction of mesothelial cells and ovarian cancer cells in the presence of TGFβ for the ... was used for the in vitro labeling of the nuclei of dividing cancer cells on HPMCs. Cancer cells that constitutively expressed ...
  http://mct.aacrjournals.org/content/13/8/2081
*  Small GTPase R-Ras regulates Integrity and Functionality of Tumor Blood Vessels
R-Ras activation in Pericytes Induces Normal Capillary-Like Vessel Morphogenesis. The physical interaction of pericytes with ... and in vitro (Figure 7B) may be attributable to enhancement of integrin-mediated cell adhesion to the extracellular matrix (ECM ... Consequently, the R-Ras deficiency greatly exacerbates the tumor-associated immature and abnormal characteristics of blood ... The expression of R-Ras38V did not affect the levels of PHD2 or HIF-2α in cultured ECs in hypoxia or normoxia (Figure S5A). ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3422514/?lang=en-ca
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... tumor cells as well as other cells.. Cancer Biology, Issue 55, tumor, vascular, endothelial, tube formation, Matrigel, in vitro ... and used them for live-cell imaging in real-time of cell:cell interactions. Our overall goal was to develop models that ... We have recently developed a 3D in vitro system to model the in vivo growth characteristics of cells that exhibit either ... We demonstrated that tumor cells that exhibit dormancy in vivo at a metastatic site remain quiescent when cultured in a 3- ...
  https://www.jove.com/visualize/abstract/22279592/erbb2-ephrinb1-src-kinase-ptpn13-signaling-complex-regulates-map