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*  Biochemistry of V(D)J Recombination | SpringerLink
The genes that encode immunoglobulin and T cell receptor proteins are assembled from component gene segments in a reaction ... ClpX protein of Escherichia coli activates bacteriophage Mu transposase in the strand transfer complex for initiation of Mu DNA ... Levchenko I, Luo L, Baker TA (1995) Disassembly of the Mu transposase tetramer by the ClpX chaperone. Genes Dev 9:2399-2408 ... Thompson CB (1995) New insights into V(D)J recombination and its role in the evolution of the immune system. Immunity 3:531-539 ...
  https://link.springer.com/chapter/10.1007/3-540-26363-2_4
*  Isolation and characterization of Ca2+-blind mutants of Yersinia pestis.
Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control ... Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. 1979 May;6(1):23-28. [PubMed] ... A multipurpose cloning system. Gene. 1977;2(2):95-113. [PubMed]. *Bradford MM. A rapid and sensitive method for the ... Genetic analysis of the low calcium response in Yersinia pestis mu d1(Ap lac) insertion mutants. J Bacteriol. 1984 Dec;160(3): ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC214309/?lang=en-ca
*  ASMscience | Site-specific DNA Inversion by Serine Recombinases
... particularly with regards to recent advances in our understanding of the function of the Fis/enhancer regulatory system. These ... on the extensively studied serine DNA invertases that are stringently controlled by the Fis-bound enhancer regulatory system. ... Purification and properties of the Escherichia coli host factor required for inversion of the G segment in bacteriophage Mu. J ... A gene for DNA invertase and an invertible DNA in Escherichia coli K-12. Gene 34:343-350. [PubMed][CrossRef]. ...
  http://www.asmscience.org/content/journal/microbiolspec/10.1128/microbiolspec.MDNA3-0047-2014
*  Nomenclature | Journal of Bacteriology
An insertion of an E. coli his gene into plasmid pSC101 at zero kilobases (0 kb) is shown as pSC101 Ω(0kb::K-12hisB)4. An ... The system of designating transposon insertions at sites where there are no known loci, e.g., zef-123::Tn5, has been described ... Binary names, consisting of a generic name and a specific epithet (e.g., Escherichia coli), must be used for all microorganisms ... Nomenclature of transposable elements (insertion sequences, transposons, and phage Mu, etc.) should follow the recommendations ...
  https://jb.asm.org/content/nomenclature
*  Frontiers | Commentary: Programmable base editing of A·T to G·C in genomic DNA without DNA cleavage | Genetics
... genes, which usually are found adjacent to the CRISPR locus. The wild-type Cas9 endonuclease and its different variants (Jinek ... exploits a modified version of the CRISPR-Cas9 system and an RNA-based deamination enzyme.INTRODUCTION: GENOME-EDITING ... commonly resulting in random insertions or deletions (indels) of nucleotides at the location of DSBs... ... gene function, and protein networks (Doudna and Charpentier, 2014; Zhang et al., 2014; Amitai and Sorek, 2016). This function ...
  https://www.frontiersin.org/articles/10.3389/fgene.2018.00021/full
*  Binary option trading free account
Lac- tose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage in vivo probe for transcription binary ... In vitro methylation of Escherichia coli 16S rRNA by tRNA(mU54)-methyltransferase. ... As described above, the product of conservative intermolecular transposition is a simple insertion (Fig. Role of binary option ... Sugar transport by the bacterial phosphotransferase system.. 40 OPTICAL AND PHYSICAL PROPERTIES FIGURE 29 1992. McGrath, P. ...
  http://imbc-med.ru/binary-option-trading-free-account-1.html
*  Bacterial Genetics Flashcards by John McClatchy | Brainscape
Consider conjugation in Escherichia coli. In which of the following matings would chromosomal genes be transferred most ... A transduction by a dsDNA phage Mu. B conjugation of a linear piece of DNA. C transformation of a linear piece of DNA. D gene ... Why does a complex transposon often contain an extra piece of DNA between the two insertion elements?. A It is often a gene ... A: The bacteriophage does not erupt from an infected cell during transduction.. B: The bacteriophage takes fragments of the ...
  https://www.brainscape.com/flashcards/bacterial-genetics-5623466/packs/8402273
*  Index of genetics articles - Wikipedia
... mating Rare-cutter enzyme Reading frame Readthrough Reannealing Rec system Receptor element Recessive allele Recessive gene ... medium Enucleate cell Enzyme Epigenetics Episome Epistasis Equational division Equivalence group Erythrocytes Escherichia coli ... Mosaicism Mouse model mRNA mtDNA Mu particle Mu phage Mullerian mimicry Multifactorial Multihybrid Multimeric structure ... mutant Axoneme B form DNA Bacillus Back mutation Backcross Bacteria Bacterial conjugation Bacterial lawn Bacteriophage Balbiani ...
  https://en.wikipedia.org/wiki/Index_of_genetics_articles
*  L-serine degradation in Escherichia coli K-12: cloning and sequencing of the sdaA gene. - Semantic Scholar
... and L-leucine has been isolated by lambda plac Mu insertion and shown to be deficient in L-serine deaminase activity. The ... The evidence is consistent with the hypothesis that sdaA is the structural gene for L-serine deaminase. However, other ... corresponding gene, sdaA, has been cloned from a prototrophic strain, and the clone has been characterized and sequenced. ... A new mutant of Escherichia coli K-12 unable to grow with L-serine, glycine, ...
  https://www.semanticscholar.org/paper/L-serine-degradation-in-Escherichia-coli-K-12-clon-Su-Lang/f10f7fb1d3a9f9773e6daeed99cad2f7cdc3e880
*  Chemical perturbation of an intrinsically disordered region of TFIID distinguishes two modes of transcription initiation | eLife
Recombinant proteins were expressed in Escherichia coli and purified according to manufacturer suggested methods. GST-fusion ... reporter gene. The 'L' DNA template had an extra 37 base-pair insertion after the promoter. The G-less cassette DNA template ... The original Hawley paper used it to block subsequent initiation in a crude system and the mechanism was assumed to be ... A (His)6-Halo-tagged reference protein (PP7 bacteriophage coat protein [Chao et al., 2008]) and a (His)6-Halo-tagged hTAF2 (990 ...
  https://elifesciences.org/articles/07777
*  RECOMBINATIONAL CLONING USING NUCLEIC ACIDS HAVING RECOMBINATION SITES - Patent application
... bacteriophage lytic genes such as those from ΦX174 or bacteriophage T4; antibiotic sensitivity genes such as rpsL, ... Moreover, functional data gathered from IS231A in Escherichia coli indicate a non-replicative mode of transposition, with a ... Most of the genes coding for these toxin proteins are plasmid-borne and are generally structurally associated with insertion ... 0273]The attB sites of the bacteriophage λ integration system were examined as an alternative to loxP sites, because they are ...
  http://www.patentsencyclopedia.com/app/20090186386
*  Protocols and Video Articles Authored by Jason E. Sandler (Translated to Arabic)
Mu Insertions Are Repaired by the Double-strand Break Repair Pathway of Escherichia Coli PLoS Genetics. 2012 , Pubmed ID: ... We also identified a number of patterns of shared gene expression, as well as genes and/or exons expressed sex-specifically or ... 22511883 Mu is both a transposable element and a temperate bacteriophage. During lytic growth, it amplifies its genome by ... Central nervous system (CNS) metastases are a common occurrence among patients with lung cancer and confer significant ...
  https://www.jove.com/author/Jason+E._Sandler?language=Arabic
*  A Tetrahydrofolate-Dependent O-Demethylase, LigM, Is Crucial for Catabolism of Vanillate and Syringate in Sphingomonas...
E. coli BL21(DE3) harboring both pELM and pG-KJE7, which carries the dnaK-dnaJ-grpE and groEL-groES genes, was grown in LB ... Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189:113-130. [ ... Plasmids of Escherichia coli as cloning vectors. Methods Enzymol. 68:245-267. [PubMed] ... Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc. Natl. ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC1064056/?lang=en-ca
*  ASMscience | Bacteriophage Therapy an
The spontaneous production of CampMu bacteriophages after bacteriophage therapy is of concern because Mu bacteriophages are ... coli colonizing poultry and contaminating poultry meat products. All the phages reported by investigators in two studies had ... Attempts to utilize bacteriophage, initially for typing purposes and more recently for their biocontrol potential, have led to ... It should be noted that bacteriophage can shape the evolution of Campylobacter genomes as they do in other bacterial genera. ...
  http://www.asmscience.org/content/book/10.1128/9781555815554.ch38
*  Transgenesis upgrades for Drosophila melanogaster | Development
A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of ... Gene insertion and replacement in Schizosaccharomyces pombe mediated by the Streptomyces bacteriophage phiC31 site-specific ... Senecoff, J. F., Rossmeissl, P. J. and Cox, M. M. (1988). DNA recognition by the FLP recombinase of the yeast 2 mu plasmid. A ... First, the insertion often disrupts another gene that may or may not be relevant (e.g. within the same pathway) to the gene ...
  http://dev.biologists.org/content/134/20/3571
*  Overproduction of Bacillus amyloliquefaciens extracellular glutamyl-endopeptidase as a result of ectopic multi-copy insertion...
Ten copies of an mpr B.amy cassette in which the GSP gene was placed between the promoter of the B. amyloliquefaciens rplU-rpmA ... As a rule, however, it is necessary to incorporate many copies of a key gene into a chromosome to achieve strain performance ... This new method is based on the construction of DNA fragments in which the desired gene, marked by antibiotic resistance, is ... A plasmid-less B. subtilis JE852-based strain secreting glutamyl-specific protease (GSP-the protein product of the mpr gene ...
  https://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-10-64
*  Most recent papers in the journal Molecular Biotechnology | Read by QxMD
Escherichia coli has been used for recombinant protein production for many years. However, no native E. coli promoters have ... We analyzed the distribution pattern of Mu insertions in teosinte (wild relative), sorghum (distant relative), and domesticated ... Gene clone experiment showed that the BJMY-CSBV-VP1 gene sequence comprised 945 bp, encoding 315 amino acids with relative ... As a cell-free display system, ribosome display has been well developed with many outstanding achievements for over 20 years. ...
  https://www.readbyqxmd.com/journal/31660
*  Binary trading easy
Insertion sequence-related genetic variation in resting Escherichia coli K-12. Bacteriol. Biochem. In M. Ferrienterobactin ... The first use of Mu tradin a genetic tool was in the study of genes needed for lysine and diaminopimelic acid synthesis (51). ... transport in Escherichia coli, p. A typical optical system for such an instrument is shown in Fig. Keepat0°Cfor4h. In a sense, ... Typing Salmonella typhimurium by means of bacteriophage. As reviewed by Sueoka (9195), these analyses of base composition ...
  http://imbc-med.ru/binary-trading-easy-5.html
*  ASMscience | Prophage Arsenal of Salm
... which is encoded by a chromosomal gene. A disruption of the grvA locus was shown to render S. enterica serovar Typhimurium more ... Early studies also indicated that some genes of certain prophages escape lysogenic repression and express functions that modify ... DNA inversions in the chromosome of Escherichia coli and in bacteriophage Mu: relationship to other site-specific recombination ... 1997.The invasion- associated type-III protein secretion system in Salmonella. Gene 192:51-59.. ...
  http://www.asmscience.org/content/book/10.1128/9781555816506.chap8
*  US Patent # 4,871,670. Molecular cloning and characterization of a gene sequence coding for human relaxin - Patents.com
... including genes and transfer vectors for expression of human prorelaxin and the individual A, B and C pepti ... Genes and DNA transfer vectors for the expression of human preprorelaxin; sub-units thereof, ... One preferred microorganism is the very familiar Escherichia coli, but any other suitable microorganism may be used. According ... mu.g) by boiling in 20 .mu.l of H.sub.2 O for 2 minutes. Synthesis was initiated by the addition of a 30 .mu.l reaction mixture ...
  http://patents.com/us-4871670.html
*  ASMscience | Studying Evolution Using
The bacterial origin of genes responsible for fungal invasion of ruminant intestinal tracts was also evident without genome ... As bacteria reproduce by binary fission, homologous recombination between genes found among closely related strains was once ... with little gain of new genes. The chapter focuses on inference of phylogeny and organismal evolution, and new biological ... Genes certainly change by point mutation, and it is primarily this sort of variation among genomes that has been under scrutiny ...
  http://www.asmscience.org/content/book/10.1128/9781555815622.ch02
*  Binary options brokers india
59, 9. The knife-edge scan involves a similar computation, characterization, and mapping of the Escherichia coli htrA gene, ... B) Host range variation of bacteriophage Mu. The quantum efficiency at any wavelength can be calculated from the formula 1239. ... generated during plasmid replication are similar to those generated in the reaction pathways that lead to random insertion. ... 26 x Cholera 27 Enterotoxigenic 28 Escherichia coli x Poliovirus 30 x Influenza virus 31 x Rabies virus 32 x Binary option ...
  http://imbc-med.ru/binary-options-brokers-india-7.html
*  Indian Patents. 256867:METHOD FOR MODIFYING PLANT MORPHOLOGY, BIOCHEMISTRY AND PHYSIOLOGY COMPRISING EXPRESSION OF PLANT...
... the FLP/FRT system of yeast, the Gin recombinase of phage Mu, the Pin recombinase of E. coli, the PinB, PinD and PinF from ... insertion mutagenesis (e.g. T-DNA insertion or transposon insertion) or by gene silencing strategies as described by e.g. ... Hanahan, D. (1983). Studies on transformation of Escherichia coli with plasmids. J.Mol.Biol 166, 557-580.. Hansen, G. and ... and intermolecular site-specific recombination in plant cells mediated by bacteriophage P1 recombinase. Gene 91, 79-85.. Dodds ...
  http://www.allindianpatents.com/patents/256867-method-for-modifying-plant-morphology-biochemistry-and-physiology-comprising-expression-of-plant-cytokinin-oxidase
*  Difference between revisions of "E. coli genotypes" - OpenWetWare
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol. 1980 Apr;138(2):179-207. PubMed ID: ... Mu) = Mu prophage present. Muδ means the phage is defective.. *mutS - mutation inhibits DNA repair of mismatches in ... E. coli B derivative strains contain an hsdRMS system (EcoBI) restricting and protecting the sequence TGA*[N8]TGCT or AGCA*[N8] ... Strain is resistant to T1 bacteriophage." F′ {proAB+ lacIq lacZΔM15 Tn10(TetR) Δ(ccdAB)} mcrA Δ(mrr-hsdRMS-mcrBC) φ80(lacZ)ΔM15 ...
  https://openwetware.org/wiki/?title=E._coli_genotypes&diff=prev&oldid=195275
*  Kudos - helping increase the reach and impact of research
Silent Mischief: Bacteriophage Mu Insertions Contaminate Products of Escherichia coli Random Mutagenesis Performed Using ... Identification of Commensal Escherichia coli Genes Involved in Biofilm Resistance to Pathogen Colonization. Published in:PLoS ... A Short-Time Scale Colloidal System Reveals Early Bacterial Adhesion Dynamics. Published in:PLoS Biology. Publication date:2008 ... CpxR/OmpR Interplay Regulates Curli Gene Expression in Response to Osmolarity in Escherichia coli. Published in:Journal of ...
  https://www.growkudos.com/profiles/17861