*  Studies on Cytotoxicity Generated in Human Mixed Lymphocyte Cultures | The Journal of Immunology
2) The AK and allo targets did not cross-compete in cold target inhibition tests. 3) AK and allocytotoxicity distributed ... Studies on Cytotoxicity Generated in Human Mixed Lymphocyte Cultures. Janet K. Seeley, Giuseppe Masucci, Anna Poros, Eva Klein ... Studies on Cytotoxicity Generated in Human Mixed Lymphocyte Cultures. Janet K. Seeley, Giuseppe Masucci, Anna Poros, Eva Klein ... Studies on Cytotoxicity Generated in Human Mixed Lymphocyte Cultures Message Subject (Your Name) has forwarded a page to you ...
  http://www.jimmunol.org/content/123/3/1303
*  Cytotoxic responses to alloantigens in systemic lupus erythematosus.
Lupus Erythematosus, Systemic / blood, drug therapy, immunology*. Lymphocyte Activation. Lymphocyte Culture Test, Mixed. ... Mixed lymphocyte reaction (MLR) assays conducted in parallel in these patients were decreased but to a lesser extent. Some of ... Plasma from most SLE patients tested did not have an effect on CML and MLR responses of normal MNC; the plasma of only one ... Title: Journal of clinical immunology Volume: 1 ISSN: 0271-9142 ISO Abbreviation: J. Clin. Immunol. Publication Date: 1981 Oct ...
  http://www.biomedsearch.com/nih/Cytotoxic-responses-to-alloantigens-in/19338096.html
*  Human T cell recognition of cloned HLA class I gene products expressed on DNA transfectants of mouse mastocytoma P815 - Serval
... genetics/immunology Lymphocyte Activation Lymphocyte Culture Test, Mixed Mast-Cell Sarcoma/*genetics/immunology Mice T- ... Human CTL generated in unidirectional mixed lymphocyte culture against AW24+ donor lymphocytes clearly lysed P815-HLA-AW24+ ... MHC class I gene products were apparently expressed on P815 transfectants in a form recognized by human cytolytic T lymphocytes ...
  https://serval.unil.ch/notice/serval:BIB_53B93F00A945
*  Fritz Bach - Wikipedia
The mixed lymphocyte culture (MLC) test as it was called, was an important step in transplant science. It was based on study of ... He was considered one of the pioneers in the field of transplant immunology. Being born in a Jewish family in Vienna, Bach fled ... One of his main achievements was the development of the mixed lymphocyte culture (MLC) test, which furthered research on ... By 1975 Bach had improved his technique so that the test would not take days anymore, but hours. This led to the possibility to ...
  https://en.wikipedia.org/wiki/Fritz_Bach
*  Immunosuppressive activity of cyclosporine metabolites in vitro - Publikationsserver der Universität Regensburg
Lymphocyte Culture Test, Mixed. MESH. Receptors, Antigen, T-Cell/immunology. MESH. T-Lymphocytes/immunology. MESH. ...
  https://epub.uni-regensburg.de/14746/
*  NAVER Academic | Mouse lymphoblasts lose their immunogenicity and susceptibility to specific cytotoxic T lymphocyte lysis...
... the cultured cells were unable to stimulate allogeneic spleen cells in mixed lymphocyte culture nor could they induce a ... We suggest that the extended survival in an allogeneic tumour‐bearing host of cultured, hence weakly immunogenic, anti‐tumour ... We demonstrate that mouse lymphoblasts (LB) lost their susceptibility to specific cytotoxic T lymphocyte (CTL)‐mediated lysis ... following culture for more than 5 days in the presence interleukin‐2 (IL‐2), IL‐7 but not IL‐4. In contrast, the cultured ...
  https://academic.naver.com/article.naver?doc_id=141288372
*  Frontiers | Recent Developments in Cellular Immunotherapy for HSCT-Associated Complications | Immunology
... in a mixed lymphocyte reaction for 7 days followed by incubation with recipient's skin for further 3 days. Then skin biopsies ... Culture Systems and Product Release. Classically, MSC expansion is performed in open culture systems using numerous plastic ... In practice, alloreactivity testing of the donor material versus the recipient material is not feasible due to the time it ... B lymphocytes secrete antigen-presenting vesicles. J Exp Med (1996) 183(3):1161-72. doi:10.1084/jem.183.3.1161 ...
  https://www.frontiersin.org/articles/10.3389/fimmu.2016.00500/full
*  Frontiers | miR-148a-3p Mediates Notch Signaling to Promote the Differentiation and M1 Activation of Macrophages | Immunology
Mixed Lymphocyte Reaction. BM-derived macrophages were seeded in 24-well plates, treated with different stimuli for 24 h, and ... test, paired t-test or one way ANOVA with Turkey's multiple comparison tests was used for statistical comparisons. P , 0.05 was ... Cell Culture and Transfection. Mouse BM cells were isolated from femurs and tibias. Nucleated cells were cultured in Dulbecco's ... After culturing with GM-CSF for 4 days, cells were analyzed by FACS. The results showed that the number of macrophages (CD11b+ ...
  https://www.frontiersin.org/articles/10.3389/fimmu.2017.01327/full
*  Measurement of Proliferative Responses of Cultured Lymphocytes - Current Protocols
Measurement of proliferative responses of human lymphocytes is a fundamental technique for the assessment of their biological ... Alternate Protocol 1: Culture for One‐Way Mixed Lymphocyte Reaction. *Alternate Protocol 2: Culture for Autologous Mixed ... Immunology 112:509‐520. Tasker, L. and Marshall‐Clarke, S. 2003. Functional responses of human neonatal B lymphocytes to ... The MTT‐assay as a rapid test for cell proliferation and cell killing: Application to human peripheral blood lymphocytes (PBL ...
  http://www.currentprotocols.com/WileyCDA/CPUnit/refId-im0710.html
*  Methods and antibodies for the immune capture and detection of Borrelia burgdorferi - DORWARD; DAVID W.
... burgdorferi recovered from two of four mixed cultures. 2 B. burgdorferi recovered from three of four mixed cultures. EXAMPLE 11 ... Mixed populations of the resulting hybrid cells are tested for their ability to secrete antibody of the desired specificity, ... 92:403-413; Umetsu, D. T., R. S. Geha (1987), In vitro production of antibody in cultures of human peripheral blood lymphocytes ... 1986, In: Handbook of Experimental Immunology: Applications of Immunological Methods in Biomedical Sciences, 4th ed. (ed. D. M ...
  http://www.freepatentsonline.com/5403718.html
*  Frontiers | Characterization and Functional Properties of Gastric Tissue-Resident Memory T Cells from Children, Adults, and the...
Additionally, all volunteers were confirmed to be H. pylori negative as determined by culture and rapid Urease test (CLO test). ... Lymphocytes in the human gastric mucosa during Helicobacter pylori have a T helper cell 1 phenotype. Gastroenterology (1998) ... of collagenase D in the digestion mix on surface marker expression the above procedure was followed using an enzymatic mix in ... The 5th most cited journal in Immunology. Frontiers in Immunology Mucosal Immunity ...
  https://www.frontiersin.org/articles/10.3389/fimmu.2014.00294/full
*  Shoshana Levy | Stanford Medicine Profiles
When culture supernatants from various cell lines were tested, Leu-2 antigen, but not Leu-1 or Leu-3, was found to be present. ... HCV is also the major cause of mixed cryoglobulinemia, a B-lymphocyte proliferative disorder. Direct experimentation with ... IMMUNOLOGY Vences-Catalan, F., Rajapaksa, R., Levy, S., Santos-Argumedo, L. 2012; 137 (1): 48-55 Abstract. In B lymphocytes, ... Ligation of TAPA-1 (CD81) or major histocompatibility complex class II in co-cultures of human B and T lymphocytes enhances ...
  https://med.stanford.edu/profiles/shoshana-levy?tab=teaching
*  Patente US5441943 - Uses of aloe products - Google Patentes
The method finds use in tissue cultures, animals and plants. ... Mixed lymphocyte cultures are in vitro models of the manner in ... cell-cell interaction response to alloantigen presented in the mixed lymphocyte culture. This model tests the capacity of ... Dorries, European Journal of Immunology, 4:230-233 (1974); Sugawara et al., Cell Immunology, 74:162-171 (1982). ... 3. Mixed Lymphocyte Cultures (MLC).. Unidirectional MLC were set up in microtiter, flat-bottom tissue culture plates (Costar Co ...
  http://www.google.es/patents/US5441943
*  Biology Major | Assumption College | Worcester, MA
... mixed lymphocyte reactions; and hemolytic plaque assays. Three lectures and one laboratory per week. Lab Fee: $400.00. ... BIO 410 INTRODUCTORY IMMUNOLOGY The focus of this course is the biology of the immune response. Topics include immunoglobulin ... Laboratory exercises will enable practical skills to be gained in measuring and testing for physiological markers of human ... In the laboratory, students learn tissue culture and such immunologic techniques as SDS gel electrophoresis; Western blotting; ...
  https://www.fe.assumption.edu/academics/programs/natural-sciences/bachelors-degree-in-biology
*  ASMscience | Tuberculosis and Nontube
Cultures are essential to exclude the possibility of mixed infections, which exist although are rare; in some instances, for ... The tuberculin skin test (Mantoux) is the test of choice for diagnosing tuberculosis infection in pregnant women. These tests ... Chapter 3 : Pathophysiology and Immunology * Authors: Arthur M. Dannenberg, Jr., Paul J. Converse ... Activated T lymphocytes and macrophages form granulomas. Granulomas inhibit replication and spread of organisms. The chapter ...
  http://www.asmscience.org/content/book/10.1128/9781555817138
*  Protocols and Video Articles Authored by Tobias Deuse (Translated to Hebrew)
The net functional immunosuppressive efficacy of MSCs was tested in mixed lymphocyte cultures. Although both clMSCs and bmMSCs ... Transplant Immunology. *The Journal of Heart and Lung Transplantation : the Official Publication of the International Society ... only CL-MSCs were able to reduce the release of IFN-γ by lymphocytes in a mixed lymphocyte reaction. In summary, CL-MSCs showed ... Sex Differences in Immunology: More Severe Development of Experimental Pulmonary Hypertension in Male Rats Exposed to Vascular ...
  https://www.jove.com/author/Tobias_Deuse?language=Hebrew
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Researchers can and do test the effects of sex steroid treatment in mixed sex neuronal cultures in models of neuronal injury ... In this article we demonstrate a rapid and minimally invasive procedure for the analysis of exercise-induced lymphocyte ... Immunology, Issue 48, Leukocyte phenotyping, programmed cell death, muscular activity, technique development ... Thus, mixed sex neuronal cultures might obscure and confound the experimental results; important information might be missed. ...
  https://www.jove.com/visualize/abstract/24586293/discrepancy-between-exercise-performance-body-composition-sex-steroid
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Immunology, Issue 67, Mucosal immunity, sexually transmitted diseases, genital tract lymphocytes, lymphocyte isolation, flow ... Figure 1. Detection schematic. The phage are mixed with the sample, the phage infects the cell, luxAB are expressed, and the ... To use this system, it is necessary for the researcher to learn how to prepare the bacterial culture and gavage the animals. We ... The ability of dendritic cells to respond to the signals required for IL-1β secretion can be tested using a synthetic purine, ...
  https://www.jove.com/visualize/abstract/23977019/plasmacytoid-dendritic-cells-are-crucial-bifidobacterium-adolescentis
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Immunology, Issue 10, Rodent, Growth Factor, TCGF, Lymphocyte, Interleukin 2, Apoptosis, Survival, T cell line, Clone ... The B/I-activated T cells are cultured with IL-7 and IL-15 for 24 hours and then pulsed with IL-2. After 24 hours, T cells are ... It can be used to examine the mechanisms of nutrient-induced dysfunction in a variety of organs and to test the effectiveness ... Replacement of interleukin 2 by T cell growth factor (TCGF) which contains a mix of cytokines is more effective than ...
  https://www.jove.com/visualize/abstract/21437204/lean-body-mass-interleukin-18-metabolic-syndrome-apparently-healthy
*  Allogenicity of Human Cardiac Stem/Progenitor Cells Orchestrated by Programmed Death Ligand 1Novelty and Significance |...
We then determined whether hCPC or IFNγ-hCPC could induce an allogeneic response using tailored 1-way mixed lymphocyte cultures ... To test the suppressive capacity of hCPC-activated CD4+CD25highCD127low T cells, we sorted the cells from 7-day cocultures ... and PBMC from all the donors were genotyped for HLA using routine standard techniques at the Laboratory of Immunology and ... Tailored mixed lymphocyte reactions to determine T-cell allogeneic immune responses were performed as previously described by ...
  http://circres.ahajournals.org/content/112/3/451
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... in culture. Representative images during the first days of culture demonstrate the presence of a mixed cell population and ... C in the tissue culture incubator. After incubation, the lymphocytes are washed off and the adherent monocytes are cultured for ... Lot release testing for the batches of DCs is performed and must meet minimum specifications before they are injected into ... Cellular Biology, Immunology, Microbiology, Issue 26, Vaccinia, virus, infection, HeLa, TRIzol reagent, total RNA, Microarray, ...
  https://www.jove.com/visualize/abstract/21209948/pge2-induces-il-6-orbital-fibroblasts-through-ep2-receptors-increased
*  EphB Receptors Trigger Akt Activation and Suppress Fas Receptor-Induced Apoptosis in Malignant T Lymphocytes | The Journal of...
We report in this article that the EphB3 receptor is expressed by all tested malignant T lymphocytes, where it is usually ... An 80-μl aliquot of this supernatant was mixed with 10 μl of the activation mix (50% glycerol, 500 μM ATP, 60 mM MgCl2, 0.05% v ... Cell culture. T-ALL cell lines H9 and E6.1 were purchased from ATCC (Manassas, VA). Molt-16 cells were kindly provided by Dr. ... The Journal of Immunology December 1, 2011, 187 (11) 5983-5994; DOI: 10.4049/jimmunol.1003482 ...
  http://www.jimmunol.org/content/187/11/5983.full
*  Cyclooxygenase-2 Inhibition Attenuates Antibody Responses against Human Papillomavirus-Like Particles | The Journal of...
Culture conditions and reagents. Splenocytes and bone marrow cells from naive and HPV 16 VLP-vaccinated mice were cultured in ... The Journal of Immunology December 1, 2006, 177 (11) 7811-7819; DOI: 10.4049/jimmunol.177.11.7811 ... Two-tailed Student's t tests were used for comparisons of two groups, and one-way ANOVA was used for comparison of three or ... CyChrome anti-mouse B220 and FITC anti-mouse CD3 mAbs were used to determine the percentage of T and B lymphocytes in spleen ...
  http://www.jimmunol.org/content/177/11/7811
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Immunology, Issue 10, Rodent, Growth Factor, TCGF, Lymphocyte, Interleukin 2, Apoptosis, Survival, T cell line, Clone ... This method involves physically separating OPCs from a mixed glial culture derived from neonatal rodent cortices. The result is ... and hiPSC lines tested to date. Cells can be passaged and maintained for more than 90 days in culture. The strategy is ... In contrast, we examine immune signaling in mature hippocampal slice cultures since the cultures closely parallel their in vivo ...
  https://www.jove.com/visualize/abstract/24204984/essential-amino-acid-enriched-high-protein-enteral-nutrition