*  Force-Induced Polarized Mitosis of Endothelial and Smooth Muscle Cells in Arterial Remodeling | Hypertension
Tissue Fixation and Immunohistochemistry. Rabbits were euthanized with IV injection of barbiturate (pentobarbital sodium, 100 ... Wall tissue remodeling regulates longitudinal tension in arteries. Circ Res. 2002; 90: 918-925. ... In some instances, (force-induced) mitosis in one direction may drive tissue growth in the same direction or in an orthogonal ... BrdU is cleared from the circulation within 60 minutes13; however, tissues were fixed ≥24 hours after injections, after BrdU- ...
  http://hyper.ahajournals.org/content/50/1/255
*  Reduced Neurogenesis after Neonatal Seizures | Journal of Neuroscience
Tissue fixation and immunohistochemistry. After deep anesthesia with sodium pentobarbital (60 mg/kg body weight), the animals ... Immunohistochemistry was performed on free-floating sections throughout the entire extent of the hippocampus as described by ... Tissue sections were incubated with a primary antibody (diluted in PBSTG) to BrdU (mouse monoclonal, 1:1000; Boehringer ... For the BrdU immunostaining, tissue permeabilization was achieved with 1 hr incubation in 0.5% PBST (0.1 m PBS and 0.5% Triton ...
  http://www.jneurosci.org/content/21/6/2094.long
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Traditional methods of immunohistochemistry (IHC) following tissue fixation allow visualization of various cell types. These ... Thereafter, the tissue homogenate was centrifuged in bovine serum albumin to separate vessel fragments from nervous tissue. The ... The blood brain barrier (BBB) specifically regulates molecular and cellular flux between the blood and the nervous tissue. Our ... In vitro expansion of neural progenitors from fetal CNS tissue has been well characterized. Despite the identification and ...
  https://www.jove.com/visualize/abstract/23285135/gfap-expression-glia-developmental-adolescent-mouse
*  Isolation of Human Embryonic Stem Cell-Derived Teratomas for the Assessment of Pluripotency - Current Protocols
Paraformaldehyde Fixation and Preparation of Tissues for Immunohistochemistry. *Basic Protocol 5: Preparation of Tissues for ... Alternate Protocol 3: Bouin's Fixation of Teratomas. *Alternate Protocol 4: Tissue Cryopreservation and Preparation for Cryo- ... The tissues in the teratoma are analyzed histologically to determine whether the hESCs are pluripotent and form tissues derived ... and they can be analyzed by immunohistochemistry for tissue markers. Methods for these procedures are included in this unit. ...
  http://www.currentprotocols.com/WileyCDA/CPUnit/refId-sc01b04.html?quicktabs_cp=materials
*  Diagnostic Evaluation of HER-2 as a Molecular Target: An Assessment of Accuracy and Reproducibility of Laboratory Testing in...
Werner M, Chott A, Fabiano A, Battifora H. Effect of formalin tissue fixation and processing on immunohistochemistry. Am J Surg ... Tissue fixation and processing for paraffin block embedding of breast cancer tissue is associated with loss of antigenicity (43 ... in the tissue microarrays either because available tissue in the tissue block was insufficient or because only unstained tissue ... HER-2 10H8-IHC was done within 2 weeks of sectioning the tissue block or receiving unstained tissue sections. At the University ...
  http://clincancerres.aacrjournals.org/content/11/18/6598.long
*  Axonal wrapping in the Drosophila PNS is controlled by glia-derived neuregulin homolog Vein | Development
Immunohistochemistry and electron microscopy analyses. Fixation and treatment of tissues for immunohistochemistry was performed ... Following a fixation in 2% OsO4 for 1 h on ice and 2% uranyl acetate at room temperature for 30 min, specimens were dehydrated ... 2012). Polyploidization of glia in neural development links tissue growth to blood-brain barrier integrity. Genes Dev. 26, 31- ... Anti-EGF receptor antibodies had been generated but did not allow a faithful localization of the protein in larval tissues. ...
  http://dev.biologists.org/content/142/7/1336.long
*  2010 HCVcc survives temperatures warrants precautions
For tissue fixation for histology or immunohistochemistry, 10% formalin (or 4% paraformaldehyde) is routinely used. ... Glutaraldehyde is another effective protein cross-linking reagent, mostly used for fixation of tissues for electron microscopy ... It should be noted, however, the routinely used concentrations of aldehydes for fixation purposes (4% for formaldehyde and 2.5 ... Production of infectious hepatitis C virus in tissue culture from a cloned viral genome. Nat Med 2005, 11:791-796. PubMed ...
  http://hcvets.com/data/Journals/2010HCVCellLifei.htm
*  Protocol specific for GPCR LGR6 Antibody (NLS439): Novus Biologicals
"Immunohistochemistry 1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.. 2. Make 4-mm sections and ... 3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.. 4. Deparaffinize the tissues by wash drying the slides in ... 5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.. 6. Wash the slides in 2 ... Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.. 22. Wash the slides in 2 ...
  https://www.novusbio.com/support/protocols/protocol-specific-for-gpcr-lgr6-antibody-nls439.html
*  Plus it
Immunohistochemistry. Tissue fixation and confocal imaging.. Postrecording biocytin-staining and confocal imaging have been ...
  http://www.jneurosci.org/content/35/33/11634
*  480226: HER-2/neu Immunohistochemistry (IHC),... | LabCorp
FFPE tissue, preserved in 10% neutral buffered formalin, fixed for 18 to 24 hours. ASCO/CAP 2013 guidelines recommend fixation ... Please note: fixation time and fixative used must be indicated on the test request form. ... One paraffin block or five unstained, positively charged tissue slides cut at 4 to 5 microns and dried for one hour at 60°C. ... Use of fixatives other than 10% formalin, fixation times less than six hours or greater than 48 hours may not yield reliable ...
  https://www.labcorp.com/test-menu/27696/her-2-ineu-i-immunohistochemistry-ihc-gastric-paraffin
*  Effect of Formalin Fixation on Thermal Conductivity of the Biological Tissue | Journal of Biomechanical Engineering | ASME DC
"Effect of Formalin Tissue Fixation and Processing on Immunohistochemistry," Am. J. Surg. Pathol., 24 , pp. 1016-1019. [CrossRef ... Effect of Formalin Fixation on Thermal Conductivity of the Biological Tissue Nachiket M. Kharalkar, Steven C. Bauserman and ... Effect of Formalin Fixation on Thermal Conductivity of the Biological Tissue. ASME. J Biomech Eng. 2009;131(7):074508-074508-4 ... Effect of formalin fixation on thermal conductivity of the biological tissues is presented. A self-heated thermistor probe was ...
  http://biomechanical.asmedigitalcollection.asme.org/article.aspx?articleid=1426115
*  Retention of function without normal disc morphogenesis occurs in cone but not rod photoreceptors | JCB
Immunohistochemistry. Tissue fixation and sectioning were performed as previously described (Stricker et al., 2005). In brief, ... Methods used for tissue collection and processing for plastic-embedment light and electron microscopy and immunohistochemistry ... LR White-embedded tissue sections (silver-gold) were viewed with an electron microscope (100EX; JEOL). For immunohistochemistry ... b) Immunohistochemistry to label cone S-opsin (red) was performed on P30 Nrl−/− and Nrl−/−/Rds−/− retinas and imaged with laser ...
  http://jcb.rupress.org/content/173/1/59
*  Anti-Fledermaus WNT1 Antikörper für Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Top performende anti-Fledermaus WNT1 Antikörper für Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) vergleichen & ... The IHC-pro Immunohistochemistry Protocol. Tissue Preparation. Formalin fixation and embedding in paraffin wax. Tissue ... Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Produktdetails lesen ... Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. ...
  http://www.antikoerper-online.de/wnt-signalweg-pathway-10/wnt1-antibody-5581/bat-ihc-p-212997/
*  Label-free Raman monitoring of extracellular matrix formation in three-dimensional polymeric scaffolds | Journal of The Royal...
... histology and microscopy techniques are all destructive and require tissue fixation, labelling, biochemical staining or cell ... Conventional quality testing methods such as immunohistochemistry, ... 2004 Tissue engineering of autologous cartilage grafts in three-dimensional in vitro macroaggregate culture system. Tissue Eng. ... 2005 A study examining the effects of tissue processing on human tissue sections using vibrational spectroscopy. Vib. Spectrosc ...
  http://rsif.royalsocietypublishing.org/content/10/86/20130464
*  Plus it
Immunohistochemistry and identification of neuronal cell populations. After the fixation procedure, the tissue was washed in ... Tissues were placed in 4°C oxygenated sterile Krebs solution containing (in mM): 117 NaCl, 4.7 KCl, 1.2 MgCl2 6H2O, 1.2 NaH2PO4 ... In control tissue (A-C), a low proportion of NSE-positive neurons (arrowhead; A) is c-Fos-immunoreactive (arrowhead; B). This ... The tissue was pinned in the dish with the mucosa up, and the Meissner's plexus (directly under the mucosa) was carefully ...
  http://ajpgi.physiology.org/content/285/5/G1049
*  Immunohistochemistry-Paraffin protocol for Laminin Antibody (NB300-144): Novus Biologicals
The fixation is routine paraformaldehyde or formalin fixation of tissue prior to paraffin embedding. However, the staining ... Immunohistochemistry Protocol De-paraffinize: xylene x2 5 min (to remove paraffin) xylene x1 10 min 100% ethanol x2 5 min (to ... Apply 3-4 drops of Crystal/Mount to tissue sections. Spread evenly by rotation. Dry slides in a 37C oven for 1-2 hours. RECIPES ... The following volumes are for 20 tissue sections (18 test and 2 controls). Pepsin Solution Dissolve 20 mg of pepsin in 5 ml of ...
  https://www.novusbio.com/support/protocols/immunohistochemistry-paraffin-protocol-for-laminin-antibody-nb300-144.html
*  Spatiotemporal gene expression - Wikipedia
... but both immunohistochemistry and in situ hybridization must be performed in fixed tissues. Techniques that require fixation of ... This pattern is lost by the time the organism develops into a larva, but wingless is still expressed in a variety of tissues ... Immunohistochemistry involves preparing an antibody with specific affinity for the protein associated with the gene of interest ... Furthermore, since immunohistochemistry visualizes the protein generated by the gene, if the protein product diffuses between ...
  https://en.wikipedia.org/wiki/Spatiotemporal_gene_expression
*  Vibratome - Wikipedia
Decreased tissue autofluoresecence due to avoidance of formalin-fixation and paraffin embedding Less wait period from tissue ... The alternating "thick and thin" slices are a challenge because electrophysiology and immunohistochemistry experiments require ... Embedding the tissue has no effect on the tissue itself, and agar or agarose embedding can be used for both live and fixed ... Tissue section thickness ranges from 100 μm to 300 μm for live tissue, although if the section thickness chosen is too thin, no ...
  https://en.wikipedia.org/wiki/Vibratome
*  Fixation (histology) - Wikipedia
Other benefits include: Long term storage and good tissue penetration. It is particularly good for immunohistochemistry ... The size of tissue can also affect the fixation process. 1-4 mm thickness [0.5 cm] At least 15-20 times greater than tissue ... Fixation of tissue is done for several reasons. One reason is to kill the tissue so that postmortem decay (autolysis and ... The broad objective of tissue fixation is to preserve cells and tissue components and to do this in such a way as to allow for ...
  https://en.wikipedia.org/wiki/Fixation_(histology)
*  List of MeSH codes (E05) - Wikipedia
... tissue fixation MeSH E05.200.500.695 --- karyometry MeSH E05.200.500.800 --- patch-clamp techniques MeSH E05.200.750.132 --- ... immunohistochemistry MeSH E05.200.500.607.790 --- periodic acid-schiff reaction MeSH E05.200.500.607.810 --- prussian blue ... tissue fixation MeSH E05.200.875.150 --- bacteriological techniques MeSH E05.200.875.150.125 --- bacterial typing techniques ... tissue culture techniques MeSH E05.200.249.750 --- tissue engineering MeSH E05.200.500.105 --- autoradiography MeSH E05.200. ...
  https://en.wikipedia.org/wiki/List_of_MeSH_codes_(E05)
*  Immunostaining - Wikipedia
Tissue preparation or fixation is essential for the preservation of cell morphology and tissue architecture. Inappropriate or ... Immunohistochemistry or IHC staining of tissue sections (or immunocytochemistry, which is the staining of cells), is perhaps ... The membrane can then be probed using antibodies using methods similar to immunohistochemistry, but without a need for fixation ... However, some antigens will not survive even moderate amounts of aldehyde fixation. Under these conditions, tissues should be ...
  https://en.wikipedia.org/wiki/Immunostaining
*  Immunohistochemistry - Wikipedia
This requires proper tissue collection, fixation and sectioning. A solution of paraformaldehyde is often used to fix tissue, ... Furthermore, autofluorescence may be due to the nature of the tissue or the fixation method. These aspects of IHC tissue prep ... Immunohistochemistry (IHC) involves the process of selectively imaging antigens (proteins) in cells of a tissue section by ... The combination of immunohistochemistry and tissue microarrays provides protein expression patterns in a large number of ...
  https://en.wikipedia.org/wiki/Immunohistochemistry
*  Meningeal lymphatic vessels - Wikipedia
Following fixation, the dura can be dissected out of the skullcap as a single piece of tissue that can be utilized for ... I think this is what allowed us to find those vessels." To visualize the dura using immunohistochemistry, the Kipnis group ... Like peripheral lymphatic vessels, the meningeal lymphatics were shown to serve both the tissue drainage and immune cell ... Compared to peripheral lymphatic vessels, the meningeal lymphatic network is markedly less complex, with far less tissue ...
  https://en.wikipedia.org/wiki/Meningeal_lymphatic_vessels
*  Frozen tissue array - Wikipedia
Since frozen tissue sections don't go through any fixation procedures, and therefore, the DNA, RNA, and protein in frozen ... Tests commonly employed in frozen tissue array include immunohistochemistry, and in situ hybridization. MicroArray and Gene ... Frozen tissue array consists of fresh frozen tissues in which up to 50 separate tissue cores are assembled in array fashion to ... Tissue microarrays from frozen tissues-OCT technique. Methods Mol Biol. 2010, 664:73-80. "Frozen Tissue Array". BioChain ...
  https://en.wikipedia.org/wiki/Frozen_tissue_array
*  Histopathology - Wikipedia
This can be done to slides processed by the chemical fixation or frozen section slides. To see the tissue under a microscope, ... Called immunohistochemistry, this technique has greatly increased the ability to specifically identify categories of cells ... The tissue is then prepared for viewing under a microscope using either chemical fixation or frozen section. If a large sample ... Histopathological examination of tissues starts with surgery, biopsy, or autopsy. The tissue is removed from the body or plant ...
  https://en.wikipedia.org/wiki/Histopathology