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*  Frontiers | Complement Evasion Strategies of Viruses: An Overview | Microbiology
... cleaving complement proteins by encoding protease, and inhibiting the synthesis of complement proteins. Additionally, viruses ... cleaving complement proteins by encoding protease, and inhibiting the synthesis of complement proteins. Additionally, viruses ... Being a major first line of immune defense, the complement system keeps a constant vigil against viruses. Its ability to ... Being a major first line of immune defense, the complement system keeps a constant vigil against viruses. Its ability to ...
  https://www.frontiersin.org/articles/10.3389/fmicb.2017.01117/full
*  Viruses | Free Full-Text | Molecular Signatures of Hepatitis C Virus (HCV)-Induced Type II Mixed Cryoglobulinemia (MCII) | HTML
... the involvement of complement factors and the specific role of some HCV proteins. In this review, we will analyze the host and ... Non-enveloped hcv core protein as constitutive antigen of cold-precipitable immune complexes in type ii mixed cryoglobulinaemia ... It is cleaved by viral and host proteases, resulting in a series of structural (core, E1 and E2) and nonstructural proteins (p7 ... HCV/E2 Ag binding to the ubiquitously expressed CD81 receptor on B-cells, as part of the CD81/CD19/CD21 complex, and ...
  http://www.mdpi.com/1999-4915/4/11/2924/htm
*  Hepatitis C vaccine - Wikipedia
... genotypes 1a and 1b and targeting the HCV antigens nonstructural protein 3 (NS3) and 4A (NS4A), as well as NS4B and NS5A ... Due to the relatively conserved binding region of E2 to the CD81 receptor on the liver cells, this discovery is expected to ... Another strategy which is different from conventional vaccine is to induce the T cell arm of the immune response using viral ... One effort involved using hepatitis B core antigen modified to carry a hepatitis C protein. In a 2006 study, 60 patients ...
  https://en.wikipedia.org/wiki/Hepatitis_C_vaccine
*  Ultrastructural and Biophysical Characterization of Hepatitis C Virus Particles Produced in Cell Culture
Interaction of hepatitis C virus nonstructural protein 5A with core protein is critical for the production of infectious virus ... HCV immune complexes that have been purified by protein A affinity chromatography contain HCV RNA, core protein, triglycerides ... Viral antigen quantification. Relative core protein levels were determined by enzyme-linked immunosorbent assay (ELISA) as ... Identification of a residue in hepatitis C virus E2 glycoprotein that determines scavenger receptor BI and CD81 receptor ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2953183/?lang=en-ca
*  In Vitro Systems for the Study of Hepatitis C Virus Infection
The structural proteins comprise the building blocks for the virion and the nonstructural proteins replicate the viral RNA [7 ... Naïve HepG2 cells do not express CD81; however, complementation with exogenous CD81 (HepG2-CD81) induces susceptibility to HCV ... An innate immune response may reduce HCV activity at a post entry level to varying degrees in the different cell types. ... PH5CH and HuS-E cells were generated by immortalizing primary hepatocytes with the T antigen of simian virus 40 and the E6/E7 ...
  https://www.hindawi.com/journals/ijh/2012/292591/
*  An overview of HCV molecular biology, replication and immune responses | Virology Journal | Full Text
... replication and immune responses against HCV and discussed how HCV escape from adaptive and humoral immune responses. This ... Nonstructural proteins. NS2. NS2 protein is a 21-23 kDa transmembrane protein. NS2 protein is essential for completion of the ... Soluble forms of CD81 can inhibit entry of HCVpp to cells [14]. Ectopic expression of CD81 in CD81-negative cells does not ... CD4+ T cells recognize antigens presented by MHC class II molecules on the surface of professional antigen presenting cells ( ...
  https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-8-161
*  A robust model of natural hepatitis C infection using hepatocyte-like cells derived from human induced pluripotent stem cells...
HLCs infected with either JFH-1 or wild-type HCV expressed HCV core antigen, NS5A, NS5B, NS3 and HCV negative-stand RNA. HLCs ... SEC14L2, an α-tocopherol transfer protein, was expressed in HLCs, but not in Huh7 cell, had been implicated in effective HCVser ... CD81, ApoE, ApoB, LDL-R) and HCV essential host factors (miR-122 and SEC14L2) comparable to the primary human hepatocyte. ... 8a-c). The cytoplasmic localization of non-structural proteins: NS3A, NS5A, NS5B and structural protein: HCV core antigen was ...
  https://virologyj.biomedcentral.com/articles/10.1186/s12985-016-0519-1
*  JCI - Hepatic expansion of a virus-specific regulatory CD8+ T cell population in chronic hepatitis C virus infection
Inhibition of natural killer cells through engagement of CD81 by the major hepatitis C virus envelope protein. J. Exp. Med. ... Antigen specificity of regulatory CD8+ LILs. In an attempt to define the antigen specificity of the regulatory cells, we ... HLA-A2 tetramers in complexes with HCV nonstructural protein 31073-1081 (NS31073-1081) (CINGVCWTV), NS31406-1415 (KLVALGINAV), ... Viral immune evasion due to persistence of activated T cells without effector function. J. Exp. Med. 1998. 188:2205-2213. View ...
  https://www.jci.org/articles/view/20515
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
The nonstructural protein 3 (NS3) from the hepatitis C virus processes the non-structural region of the viral precursor ... which lacks endogenous expression of CD81, an essential entry factor of HCV. In the absence of ectopically expressed CD81, ... The magnitude of the immune response characterized by the frequency and activity of hCYP2D6-specific T and/or B cells and the ... that ensures a direct delivery of the triggering antigen to the liver. Thus, the ensuing local inflammation generates a fertile ...
  https://www.jove.com/visualize/abstract/23936097/allosteric-inhibitors-of-the-ns3-protease-from-the-hepatitis-c-virus
*  С ?? ҩ ??.??Ϣ?? .11202501??11203000
11202652:G-Protein Signaling Triggered by R5 Human Immunode. *11202653:Characterization of Nonstructural Protein Membrane ... 11202916:Characterization of Immune Responses Induced by In. *11202917:Chimeric Influenza Virus Hemagglutinin Proteins Co ... 11202945:Epstein-Barr Virus Nuclear Antigen 2(EBNA2) Gene. *11202946:An S101P Substitution in the Putative Cleavage Mot ... 11202738:Hepatitis C Virus E2-CD81 Interaction Induces Hype. *11202739:Molecular Interactions of Human Immunodeficiency V ...
  http://www.100md.com/index/other/byinfono/022/406.htm
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Agrobacterium-mediated transient protein production in plants is a promising approach to produce vaccine antigens and ... of which ORF1 occupies most of the genome and encodes seven non-structural proteins (NS1-7) released from a polyprotein ... which lacks endogenous expression of CD81, an essential entry factor of HCV. In the absence of ectopically expressed CD81, ... Recently, we have discovered that γHV68 usurps an innate immune signaling pathway to promote viral lytic replication5. ...
  https://www.jove.com/visualize/abstract/22216177/replication-gene-expression-particle-production-consensus-merkel-cell
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Agrobacterium-mediated transient protein production in plants is a promising approach to produce vaccine antigens and ... of which ORF1 occupies most of the genome and encodes seven non-structural proteins (NS1-7) released from a polyprotein ... which lacks endogenous expression of CD81, an essential entry factor of HCV. In the absence of ectopically expressed CD81, ... Additionally, the vector immune response has been described using two strains of SINV engineered to express GFP2,9. Here, we ...
  https://www.jove.com/visualize/abstract/21818349/an-internal-ribosome-entry-site-directs-translation-3-gene-from
*  CiNii 論文 - Advances in Genomic Research on Hepatitis C Virus with a Useful Tool, Replicon...
Hepatitis C virus non-structural proteins in the probable membranous compartment function in viral genome replication MIYANARI ... The role of CD81 and glycosaminoglycans in the adsorption and uptake of infectious HCV particles MORIKAWA K ... Benefit of hepatitis C virus core antigen assay in prediction of therapeutic response to interferon and ribavirin combination ... Implication for the E7-mediated immune evasion mechanism in cervical carcinogenesis PARK JS ...
  http://ci.nii.ac.jp/naid/10021988439
*  Viruses | Free Full-Text | Neutralizing Antibodies and Pathogenesis of Hepatitis C Virus Infection | HTML
The interplay between the virus and host innate and adaptive immune responses determines the outcome of infection. There is ... These include scavenger receptor class B type 1 (SR-BI), CD81, the tight junction proteins claudin 1 (CLDN1) and occludin (OCLN ... Adaptive immune responses are mediated by the humoral and cellular immune systems. HCV-specific T lymphocytes are detectable 5 ... Hepatitis C virus nonstructural 5A protein induces interleukin-8, leading to partial inhibition of the interferon-induced ...
  http://www.mdpi.com/1999-4915/4/10/2016/htm
*  Binding of the Hepatitis C Virus Envelope Protein E2 to CD81 Inhibits Natural Killer Cell Functions
HCV-encoded proteins such as envelope protein E2, the nonstructural protein 5A, and core protein have been shown to have ... CD81 (TAPA-1): A molecule involved in signal transduction and cell adhesion in the immune system. Annu. Rev. Immunol. 16:89-109 ... Interleukin-12: a proinflammatory cytokine with immunoregulatory functions that bridge innate resistance and antigen-specific ... CD81 is a member of the tetraspan superfamily (TM4SF) of proteins (28). Results from a number of studies suggest that TM4SF ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2196015/?lang=en-ca
*  DEUTERATED NUCLEOSIDE/TIDE DERIVATIVES - Patent application
The P7 protein is of unknown function and is comprised of a highly variable sequence. There are several non-structural proteins ... cytokines that modulate immune function. Also included are vaccines comprising HCV antigens or antigen adjuvant combinations ... CD81 inhibitors, cyclophilin inhibitors, internal ribosome entry site (IRES) inhibitors or any combinations thereof. Interferon ... This large polypeptide is subsequently processed into the individual structural and nonstructural proteins by a combination of ...
  http://www.patentsencyclopedia.com/app/20160130294
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... modulation biosensing system was previously used to detect the coding sequences of the non-structural Ibaraki virus protein 3 ( ... which lacks endogenous expression of CD81, an essential entry factor of HCV. In the absence of ectopically expressed CD81, ... The method relies on heat-based antigen unmasking, and directly labeled home-made DNA probes, or commercially available probes ... Infectious Diseases, Issue 89, innate immune response, virus infection, pathogen recognition receptor, RIG-I, PKR, IRF-3, ...
  https://www.jove.com/visualize/abstract/24586263/computational-study-on-inhibitor-binding-mode-allosteric-regulation
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Immunology, Issue 80, Virus Diseases, Immune System Diseases, HIV, CD4 T cell, CD8 T cell, antigen-presenting cell, Cytokines, ... The RND and OMF proteins are transmembrane proteins. Transmembrane proteins make up more than 30% of all proteins and are 65% ... which lacks endogenous expression of CD81, an essential entry factor of HCV. In the absence of ectopically expressed CD81, ... An attenuated West Nile virus (WNV), a nonstructural (NS) 4B-P38G mutant, induced higher innate cytokine and T cell responses ...
  https://www.jove.com/visualize/abstract/21246041/hepatitis-b-virus-lacks-immune-activating-capacity-but-actively
*  US Patent for Anti-HCV monoclonal antibody as a medicament for the therapeutic treatment and prevention of HCV infections...
A recombinant human Fab (c33-3) specific for a non-structural HCV antigen (NS3) was included as a negative control in each ... Buironi, R., et al., Dissection of human humoral immune response against hepatitis C virus E2 glycoprotein by repertoire ... Non-limiting examples of molecules suitable for conjugation include human serum albumin, maltose-binding protein, glutathione-S ... Assessment of e20 Binding to E2 Glycoproteins from HCV 1a Mutated within CD81-Binding Regions ...
  https://patents.justia.com/patent/8623363
*  Microbiology research tools: primary antibodies, proteins, peptides, kits
... proteins, molecular biology reagents, kits, slides, and lysates. ... EBV Early Antigen Restricted Ea-R p85 Proteins and Peptides (1) ... Avian Influenza A Nonstructural 1 Primary antibodies (1). *Avian Influenza A Nonstructural 1 Proteins and Peptides (1) ... Host Immune Response (121)+. *BPIFB1 Primary antibodies (4). *BPIFB1 Proteins and Peptides (1) ... CD200R Proteins and Peptides (5). *CD81 Primary antibodies (21). *CD81 Proteins and Peptides (2) ...
  https://www.abcam.com/index.html?pageconfig=productmap&cl=252
*  HCV Research and News: 01/18/12
Ciesek S, Steinmann E, Wedemeyer H et al. Cyclosporine A inhibits hepatitis C virus nonstructural protein 2 through cyclophilin ... Coinfection IDU IDX184 IDX19368 IDX19370 IDX20963 IDX21437 IDX21459 IDX2963 IDX719 IL28B IL28B CC Immune disorders Immune ... and the tight junction proteins claudin-1 and occludin.[40] Interestingly, neutralizing antibodies against CD81 are able to ... the degree of human leucocyte antigen (HLA) matching[25] or the IL28b genotype of the donor and the recipient.[26,27] ...
  http://hepatitiscresearchandnewsupdates.blogspot.com.au/2012_01_18_archive.html
*  Activation of HCV Specefic T Cells - Patent application
... or after the administration of the antigen(s) to boost the immune response to that antigen. The adjuvant and protein antigen(s ... protein binding to CD81. CD81 recombinant receptor protein is coated onto 96-well plates. Anti-E2 specific monoclonal antibody ... nonstructural proteins (e.g., nsP1, nsP2, nsP3, nsP4), and 3' viral or cellular sequences required for nonstructural protein- ... The protein antigen(s) and viral vector are preferably administered sequentially with protein antigen administered at least ...
  http://www.patentsencyclopedia.com/app/20100034844
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
While the primary function of T cells is to mediate protective immune responses to foreign antigens presented by self-MHC, T ... which lacks endogenous expression of CD81, an essential entry factor of HCV. In the absence of ectopically expressed CD81, ... The HCV protein expression was verified by Western blot and immunofluorescence assays using antibodies specific for HCV NS3 and ... An attenuated West Nile virus (WNV), a nonstructural (NS) 4B-P38G mutant, induced higher innate cytokine and T cell responses ...
  https://www.jove.com/visualize/abstract/22701633/dcs-pulsed-with-novel-hla-a2-restricted-ctl-epitopes-against
*  US Patent Application for ANTI-HCV MONOCLONAL ANTIBODY AS A MEDICAMENT FOR THE THERAPUTIC TREATMENT AND PREVENTION OF HCV...
A recombinant human Fab (c33-3) specific for a non-structural HCV antigen (NS3) was included as a negative control in each ... Non-limiting examples of molecules suitable for conjugation include human serum albumin, maltose-binding protein, glutathione-S ... Assessment of e20 Binding to E2 Glycoproteins from HCV 1a Mutated within CD81-Binding Regions ... yet are all the same able to bind to different glycoproteins never encountered by the immune system of the patient selected for ...
  https://patents.justia.com/patent/20110256140
*  admin | Anti cancers
Takaki et al.108 also found that HCV non-structural proteins, particularly NS4, change the iDC Talazoparib datasheet phenotype ... A few studies have been performed by proteomics to search new specific T cell antigens for IGRA but no satisfying protein was ... To investigate the contribution of intrahepatic PDC and MDC to local immune responses during HCV infection, Lai et al.85,86 ... migration of DC is markedly affected by interaction of HCV E2 with CD81. A two-photon confocal microscopic analysis revealed ...
  https://anti-cancers.com/index.php/author/admin/