Gluconeogenesis in the kidney cortex. Effects of d-malate and amino-oxyacetate | Biochemical Journal
... inhibited strongly the glutamate-oxaloacetate transaminase in the 30000g supernatant fraction of a kidney-cortex homogenate. ... Gluconeogenesis in the kidney cortex. Effects of d-malate and amino-oxyacetate. R. Rognstad, J. Katz ... 1. Rat kidney-cortex slices incubated with d-malate alone formed very little glucose. d-Malate, however, augmented ... 8. Detritiation of l-[2-3H]aspartate was inhibited by 90% during an incubation of kidney-cortex slices with l-lactate and amino ...http://www.biochemj.org/content/116/3/483
Molecular activity of Na+/K+-ATPase from different sources is related to the packing of membrane lipids | Journal of...
... microsomes were prepared from the cortex of the kidney. Microsomes were prepared from 10 % tissue homogenates that had been ... Kidney microsomes from the rat have a lower level of total unsaturates than toad kidney microsomes, and this difference is ... These values were 789, 781, 745 and 741 Da for rat kidney, rat brain, toad kidney and toad brain, respectively. ... Both species have the same total polyunsaturate content in their kidney microsomes, although rat kidney microsomes have ...http://jeb.biologists.org/content/204/24/4271
Expression of Glutathione-Dependent Enzymes and Cytochrome P450s in Freshly Isolated and Primary Cultures of Proximal Tubular...
Isolation of Microsomes and Cytosol from Human Kidney Homogenates.. Freshly isolated slices of human kidney cortex were ... 1989) Isolation of two distinct populations of cells from rat kidney cortex and their use in the study of chemical-induced ... 1996) Anatomy of the kidney. in The Kidney, ed Brenner BM (WB Saunders Co. Philadelphia), pp 8-18. ... 1984) Tissue culture of human kidney epithelial cells of proximal tubule origin. Kidney Int 25:383-390. ...http://jpet.aspetjournals.org/content/293/2/677
Immunolocalization of NHE8 in rat kidney | Renal Physiology
The kidneys were removed, and the dissected cortex was homogenized. This homogenate was subjected to a low-speed spin (2,400 g ... Mouse kidney membranes were prepared in a similar fashion except that both cortex and medulla were included in the homogenate. ... Double-labeling of NHE8 with γ-glutamyl transpeptidase (GGT) and NHE8 with megalin in rat kidney cortex. A-D: 1 set of ... Immunofluorescence studies of rat kidney cortex. A: immunofluorescence and phase images using Rab65. B and C: ...http://ajprenal.physiology.org/content/288/3/F530
Identification and proteomic profiling of exosomes in human urine | PNAS
... were markedly enriched relative to kidney homogenates from cortex, outer medulla, and inner medulla. ... Immunoblots of proteins in human urine exosomes (Urine) and kidney regions (Cortex, Outer Medulla, and Inner Medulla). ... Kidney samples for immunoblotting were obtained from the unaffected portion of a kidney resected because of a renal tumor ( ... Xiaoyan Wang for preparing human kidney samples; Dr. Zu-Xi Yu for assistance with electron microscopy; Angel Aponte for help ...http://www.pnas.org/content/101/36/13368
Immunoperoxidase labelling of Liu-FPN1 in rat kidney. ( | Open-i
A) Strong labelling is evident throughout the cortex and outer stripe of the outer medulla. Labelling is ... Immunoperoxidase labelling of Liu-FPN1 in rat kidney. ( ... Homogenate, microsomal membranes and BLM from rat kidney cortex ... immunoreactivity in rat kidney cortex. Homogenate, microsomal membranes and BLM from rat kidney cortex were separated under ... I) FPN1 and kidney electrogenic Na-bicarbonate cotransporter-1 (NBCe1-A) immunoreactivity in rat kidney cortex. ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC3822789_jcmm0015-0209-f2a&req=4
Cytochrome P450-Derived Arachidonic Acid Metabolism in the Rat Kidney: Characterization of Selective Inhibitors | Journal of...
... and the cortex was dissected and homogenized in buffer containing 100 mM Tris-HCl and 1.15% KCl, pH 7.4. Homogenates were ... 1995) Effect of arachidonic acid on activity of the apical K+ channel in the thick ascending limb of the rat kidney. J Gen ... Cytochrome P450-Derived Arachidonic Acid Metabolism in the Rat Kidney: Characterization of Selective Inhibitors. Mong-Heng Wang ... Cytochrome P450-Derived Arachidonic Acid Metabolism in the Rat Kidney: Characterization of Selective Inhibitors. Mong-Heng Wang ...http://jpet.aspetjournals.org/content/284/3/966?ijkey=2efe7dbbff8d2e7a988deeead0c1db7cecc7b357&keytype2=tf_ipsecsha
Alagebrium Reduces Glomerular Fibrogenesis and Inflammation Beyond Preventing RAGE Activation in Diabetic Apolipoprotein E...
Protein extracts from kidney cortex for each group (n = 5-6/group) were used to estimate the concentration the expression of ... Plates were blocked with 4% milk/PBS/T for 2 h at room temperature (RT) before mouse kidney homogenates were added (1:2; 2 h RT ... Briefly, frozen cortex was minced on ice using a scalpel blade. The minced cortex was flushed through a 100-μm disposable ... Kidneys were rapidly dissected and weighed (wet weight) before both organs were snap frozen in liquid nitrogen and stored at − ...http://diabetes.diabetesjournals.org/content/61/8/2105.full
Impaired Prostaglandin E2/Prostaglandin I2 Receptor-Gs Protein Interactions in Isolated Renal Resistance Arterioles of...
... renal cortex, renal medulla, and whole-kidney homogenates.5 6 17 21 25 Similar findings are reported for isolated mesenteric ... and membranes from whole-kidney homogenates from SHR.6 13 17 21 25 Other groups have reported unchanged or increased forskolin ... Defective G protein activation of the cAMP pathway in rat kidney during genetic hypertension. Proc Natl Acad Sci U S A. 1995;92 ... Interactions of cAMP-mediated vasodilators with angiotensin II in rat kidney during hypertension. Am J Physiol. 1993;265:F845- ...http://hyper.ahajournals.org/content/34/5/1134
Expression of membrane-associated carbonic anhydrase isoforms IV, IX, XII, and XIV in the rabbit: induction of CA IV and IX...
Abbreviations: K. cortex, kidney cortex; G. bladd., gall bladder; Sk. Muscle, skeletal muscle. ... After incubation on ice for 30 min, homogenates were clarified by centrifugation at 14,000 g. Aliquots of tissue homogenates ( ... 1 and 2B). CA XII mRNA expression was restricted to kidney cortex and colon, and CAs IV and XII comprised all of the membrane- ... There was no expression of CA IX in kidney cortex. A and B: open vs. solid bars represent independent experiments from two ...http://ajpregu.physiology.org/content/288/5/R1256.long
... and then 2 mg for kidney cortex and liver ) of homogenate was incubated with 1mM ADMA for 45 min (kidney cortex) or 60min ( ... Kidney cortex CAT and cellular f ractions of PKC e xpression. (A) Membrane bound CAT expression in the kidney cortex with ... A) Kidney cortex DDAH1 and 2 expression and (B) activity assay measured by citrulline production. (C) Kidney cortex PRMT1 and ( ... in the aorta and kidney cortex. Reductions in kidney cortex CAT1 indicated to us that the proximal tubules had a reduced ...http://ufdc.ufl.edu/UFE0024922/00001
Regulation of Aldosterone Biosynthesis by Adrenal Renin Is Mediated Through AT1 Receptors in Renin Transgenic Rats |...
The adrenal and kidney cortex were immediately frozen in liquid nitrogen and kept at −70°C until RNA extraction. These tissues ... Active renin in the tissue homogenates was measured by incubating 100 μL sample with a 50 mmol sodium maleate buffer (pH 6.4) ... For the RNase protection assay, total RNA (40 μg from each kidney cortex and 2 μg from each adrenal capsule) was vacuum-dried ... Renin expression in the kidney cortex of the two experimental groups. From the left to the right, lanes 1 to 3 represent the ...http://circres.ahajournals.org/content/77/1/73
Organ-Specific Overexpression of Renal LAT2 and Enhanced Tubular l-DOPA Uptake Precede the Onset of Hypertension | Hypertension
The presence of LAT1 and LAT2 transcripts in kidney cortexes and intestinal mucosa of 12-week-old WKY and SHR animals was first ... AADC activity was determined in homogenates of renal tissues with l-DOPA (102 to 104 μmol/L) as the substrate, as previously ... Figure 4. Kidney LAT2 mRNA quantification. A, Northern blot of 10 μg total RNA from 4- and 12-week-old WKY and SHR kidneys. RNA ... Expression of LAT2 in SHR kidneys was higher than in WKY kidneys. This increase was more marked at 4 than at 12 weeks of age. ...http://hyper.ahajournals.org/content/42/4/613
Production and Actions of Superoxide in the Renal Medulla | Hypertension
When the tissue homogenates from the renal cortex, outer medulla (OM), and inner medulla (IM) were incubated with NADH and ... Tissue homogenates (20 μg) freshly prepared from the different kidney regions were incubated with dihydroethidium (DHE, 10 μmol ... was monitored when tissue homogenate from different kidney regions was incubated with substrates for the major O2−·-producing ... was detected in the reaction mixtures from all 3 kidney regions (n=6 rats). The renal cortex and OM exhibited greater NADH ...http://hyper.ahajournals.org/content/37/2/547
Glutamine synthesis and synthetase in guinea pig kidney - Memorial University Research Repository
These studies showed that the guinea pig kidney cortex tubule synthesizes glutamine rapidly at pH 7.4 (503+78μmoles/30 min/g ... since glutamine synthetase almost consistently appeared in the soluble fraction of spun homogenates, using sucrose or KC1 media ... Subcellular fractionation studies on guinea pig kidney cortex indicated an exclusive oytosolic localization of glutamine ... The guina pig was chosen as a suitable experimental animal model for the studies reported, because the guinea pig kidney has a ...http://research.library.mun.ca/4066/
Lipid Profile in Human Frontal Cortex Is Sustained Throughout Healthy Adult Life Span to Decay at Advanced Ages, The Journals...
"Lipid Profile in Human Frontal Cortex Is Sustained Throughout Healthy Adult Life Span to Decay at Advanced Ages, The Journals ... Fatty Acid Profile Fatty acids from human frontal cortex homogenates were analyzed as methyl ester derivatives (FAMEs) by gas ... kidney failure, and metabolic syndrome) and hypoxia were excluded. Following initial screening, the present series include 58 ... Fatty Acid Profile Fatty acids from human frontal cortex homogenates were analyzed as methyl ester derivatives (FAMEs) by gas ...https://www.deepdyve.com/lp/ou_press/lipid-profile-in-human-frontal-cortex-is-sustained-throughout-healthy-KUocWei0C0
Critical Role of AT1 Receptor Expression After Ischemia/Reperfusion in Isolated Rat Hearts | Circulation Research
Cloning of a rabbit kidney cortex AT1 angiotensin II receptor that is present in proximal tubule epithelium. Am J Physiol. 1993 ... The homogenate was centrifuged at 7000 rpm for 20 minutes. The supernatant was then applied to a Sep-Pak C18 cartridge, washed ... Primers were designed on the basis of the AT1R sequence cloned from the rabbit kidney cortex.30 The sense primer sequence was 5 ... Angiotensin II and aldosterone receptor binding in rat heart and kidney: response to chronic angiotensin II or aldosterone ...http://circres.ahajournals.org/content/83/5/552
TLR2 Is Constitutively Expressed within the Kidney and Participates in Ischemic Renal Injury through Both MyD88-Dependent and ...
Right panel, KC in tissue homogenates. Protein was detected in either serum or kidney homogenates using a commercial ELSIA kit ... TLR2 is highly expressed in the WT kidney, with the highest amount of TLR2 expressed within the renal cortex and the outer ... and then kidneys were harvested and halved kidneys were snap frozen in liquid nitrogen. The kidneys were homogenized in ... TLR2 expression within normal mouse kidney. Fig. 1 shows four panels (labeled A-D) and a section of WT kidney stained for H&E ...http://www.jimmunol.org/content/178/10/6252.long
Differences between the cortex, outer and inner medulla, Kidney Int., 40, 625-631.. 3.Adams, P. L., Adams, F. F., Bell, P. D., ... In SkQR1-treated animals, Evans Blue concentration after I/R in the total kidney homogenate did not differ from that of intact ... 1b). We observed the dye accumulation in the cortex and outer medulla, i.e. in the kidney sections most sensitive to ischemic ... We measured the amount of Evans Blue extracted from the total kidney homogenates and found the difference between the control ...http://protein.bio.msu.ru/biokhimiya/contents/v81/full/81129996.html
Frontiers | Nerve growth factor is primarily produced by GABAergic neurons of the adult rat cortex | Frontiers in Cellular...
... including the prefrontal cortex, primary motor cortex, parietal cortex and temporal cortex. Our results show that NGF ... including the prefrontal cortex, primary motor cortex, parietal cortex and temporal cortex. Our results show that NGF ... These findings demonstrate that GABAergic cells are the primary source of NGF production in the cortex, and likely support the ... Classification of cells producing NGF in the cortex is lacking, however, and cholinergic corticopetal projections have been ...https://www.frontiersin.org/articles/10.3389/fncel.2014.00220/full
Role of Superoxide in Modulating the Renal Effects of Angiotensin II | Hypertension
O2− Levels in Renal Cortex Homogenates. The levels of O2− in renal homogenates, as estimated by lucigenin-enhanced ... Because the kidney and specifically the pressure-natriuresis phenomenon is thought to play a central role in the long-term ... Figure 8. Superoxide (O2−) levels in renal cortex homogenates incubated with vehicle [C, control], Ang II (ANG II, 10 nmol/L), ... Protocol 10 involved superoxide levels in renal cortex homogenates (n=6). Ang II (10 nmol/L), valsartan (10 μmol/L), apocynin ( ...http://hyper.ahajournals.org/content/42/6/1150
LC3 II is increased in MnSOD KO sham kidneys. C: Western blot detection for Atg5 from renal homogenate of control kidneys. ... Seven different fields (20X) (3 cortex, 2 outer medulla, 2 inner medulla) from each mouse kidney section were considered for ... B: Western blot detection for LC3 I and II from renal homogenate of control kidneys. GAPDH was used as a loading control. Graph ... The kidney-specific MnSOD knockout (KO) mice and littermate wild-type (WT) controls (Kidney Cre) mice were used in this study ...http://ajprenal.physiology.org/content/304/3/F257
Robert K. Crane - Wikipedia
"The accumulation of D-galactose against a concentration gradient by slices of rabbit kidney cortex". Journal of Biological ... "The association of hexokinase with particulate fractions of brain and other tissue homogenates". Journal of Biological ...https://en.wikipedia.org/wiki/Robert_K._Crane
Prostaglandin E-Prostanoid4 Receptor Mediates Angiotensin II-Induced (Pro)Renin Receptor Expression in the Rat Renal...
Sodium regulation of angiotensinogen mRNA expression in rat kidney cortex and medulla. J Clin Invest. 1986;78:1311-1315. ... The homogenates were centrifuged at 4000 rpm at 4°C for 30 minutes, and the supernatant was collected. ... Increased expression of cyclooxygenase-2 in the renal cortex of human prorenin receptor gene-transgenic rats. Kidney Int. 2006; ... At day 14, under isoflurane anesthesia, blood was withdrawn from vena cava and kidneys were harvested and cut into cortex and ...http://hyper.ahajournals.org/content/64/2/369
Increased peripheral and local soluble FGL2 in the recovery of renal ischemia reperfusion injury in a porcine kidney auto...
Twenty μg protein from kidney homogenate was separated on 15% (wt/vol) poly acrylamide denaturing gels and electro-blotted onto ... MPO + cells and active caspase-3+ cells in the renal cortex were manually counted, while the expression of sFGL2 and FcγRIIB ... Donor kidney retrieving and preservation. The left kidney was mobilized and removed with minimal warm ischemia (about 1 min) ... Foxp3 and IL-10 mRNA expression in the kidney. Both Foxp3 and IL-10 mRNA in the kidney remarkably increased from day 5, ...https://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-12-53