Mixed lymphocyte reaction - Wikipedia
Lymphocyte interaction: A potential histocompatibility test in vitro. Science 143:813. 4. Benacerraf, B. and H.O. McDevitt. ... A reaction between leukocytes in mixed peripheral blood cultures. Fed. Proc. 22:4281. 3. Bach, F.H. and K. Hirschhorn. 1964. ... Mixed lymphocyte reaction (MLR) is a test used by pharmaceutical and biotech organizations to show the safety of a drug or ... "Why are Mixed Lymphocyte Reactions Performed?". Retrieved 7 January 2017. 1. Meo, T.: The MLR test in the mouse. 1979. In ...https://en.wikipedia.org/wiki/Mixed_lymphocyte_reaction
Fritz Bach - Wikipedia
The mixed lymphocyte culture (MLC) test as it was called, was an important step in transplant science. It was based on study of ... changing cell structures in vitro cultures of peripheral blood lymphocytes of unrelated individuals. Bach worked further on the ... Bach's work on transplant compatibility helped in later experiments that identified the major histocompatibility complex. In ... One of his main achievements was the development of the mixed lymphocyte culture (MLC) test, which furthered research on ...https://en.wikipedia.org/wiki/Fritz_Bach
Jan Klein - Wikipedia
... proliferation of lymphocytes in an in vitro culture challenged with H2-incompatible stimulating cell (the so-called mixed ... The tests revealed that closely related species such as M. domesticus and M. musculus, the many haplochromine fish species in ... All other histocompatibility genes came to be called minor. Initially, genetic mapping of the mouse class I antigens suggested ... The phenomenon could be reproduced in vitro by the exposure to the antigen. Lymphocytes isolated from the high responder ...https://en.wikipedia.org/wiki/Jan_Klein
Adoptive cell transfer - Wikipedia
They then produce pure cultures of lymphocytes that can be tested for reactivity against other tumors, in coculture assays. ... In vitro tests on melanoma and kidney cancer models met expectations. In 2016 Strep-tag II sequences were introduced into ... CARs can provide recognition of cell surface components not restricted to major histocompatibility complexes (MHC). They can be ... harboring rearrangement of the mixed lineage leukemia (MLL). The transfer of regulatory T cells has been used to treat Type 1 ...https://en.wikipedia.org/wiki/Adoptive_cell_transfer
Regulatory T cell - Wikipedia
In vitro experiments have given mixed results regarding the requirement of cell-to-cell contact with the cell being suppressed ... CD4+ helper T cells from skin-test positive adults killed CD8+ T cells but not CD4+ helper T cells when added to culture media ... Read, S; Malmström, V; Powrie, F (17 July 2000). "Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the ... The large majority of Foxp3-expressing regulatory T cells are found within the major histocompatibility complex (MHC) class II ...https://en.wikipedia.org/wiki/Regulatory_T_cell
AID 90482 - Percent inhibition against incorporation of [3H]-thymidine in Human mixed lymphocyte reaction at a concentration of...
BioAssay record AID 90482 submitted by ChEMBL: Percent inhibition against incorporation of [3H]-thymidine in Human mixed lymphocyte reaction at a concentration of 25 uM.https://pubchem.ncbi.nlm.nih.gov/bioassay/90482
THE MIXED LEUCOCYTE REACTION APPLIED TO INBRED MOUSE STRAINS,
The mixed leucocyte reaction, (MLR), has been applied successfully to peripheral blood leucocytes of the mouse. Before harvest, the leucocytes were mobilized into the peripheral blood by a single intravenous injection of the mice with pertussis vaccine. Mixtures, (50-50), of C57BL/6 and DBA/2 mouse leucocytes were cultured in medium containing low amounts of mouse plasma and supplemented with foetal bovine serum. DNA-synthetic activities at selected times were determined by liquid scintillation counting following pulse labeling of the cell populations with 3H-TdR. DNA synthesis in the mixed cultures attained a maximum value at the 5th to 7th day (7,500 cpm), as contrasted with maximum control values of 200-1000 cpm). Considerable DNA synthesis (1000-2000 cpm), also was observed at zero time, and then declined to low levels (200-300 cpm) at the 18th hour. DNA synthesis did not occur in the mixed leucocyte ...http://oai.dtic.mil/oai/oai?verb=getRecord&metadataPrefix=html&identifier=AD0659476
Autologous mixed lymphocyte reaction (MLR) induced in co-cultures of T-Cells and iodine preincubated thyroid epithelial cell...
Wenzel, B. E.; Bay, V.; Engel, U.; Gutekunst, R. und Scriba, Peter Christian (1986): Autologous mixed lymphocyte reaction (MLR) induced in co-cultures of T-Cells and iodine preincubated thyroid epithelial cell cultures (TEC) from patients with Graves' disease (GD). In: Immunobiology, Vol. 173: 347 A J. 45 ...https://epub.ub.uni-muenchen.de/8914/
"Detection of autologous mixed lymphocyte reaction responding cells and" by M H. Bocchieri and J B. Smith
Bocchieri, M H. and Smith, J B., "Detection of autologous mixed lymphocyte reaction responding cells and their precursor frequency in nzb mice." (1982). Subject Strain Bibliography 1982. 4506 ...https://mouseion.jax.org/ssbb1982/4506/
Natural killer cells activated in a human mixed lymphocyte res...
Natural killer cells activated in a human mixed lymphocyte response culture identified by expression of Leu-11 and class II histocompatibility antigens.: Lymphohttps://www.mysciencework.com/publication/show/natural-killer-cells-activated-human-mixed-lymphocyte-response-culture-identified-expression-leu-11-class-ii-histocompa-130db279
"Studies on the syngeneic mixed lymphocyte reaction. Iii. Development" by P B. Hausman, C E. Moody et al.
Hausman, P B.; Moody, C E.; Innes, J B.; J, J; B; and Weksler, M E., "Studies on the syngeneic mixed lymphocyte reaction. Iii. Development of a monoclonal antibody with specificity for a autoreactive t cells." (1983). Subject Strain Bibliography 1983. 1462 ...https://mouseion.jax.org/ssbb1983/1462/
Stable Down-Regulation of HLA Class-I by Serum Starvation in Human PBMCs
Background: The human leukocyte antigen (HLA) matching between organ donor and recipient is an acceptable strategy in clinical transplantation since 1964. However, in bone marrow transplantation, finding matched donors is often problematic. Thus new method for down regulation of HLA can be an alternative strategy to solve this problem. Objective: To examine the effect of serum starvation on HLA class I expression in human peripheral blood mononuclear cells (PBMCs). Methods: PBMCs were cultured in RPMI-1640 supplemented with 10% FBS (non-starved cells) as well as in medium only (starved cells) for 16, 24, 48, 72, 96h under standard cell culture conditions. The pattern of cell death and HLA class I expression was determined by flowcytometry. Antigenicity of the starved PBMCs was evaluated in a one-way mixed lymphocyte culture by MTT assay. Results: Mean fluorescence intensity (MFI) of different indicated starved PBMCs ...http://iji.sums.ac.ir/article_16728.html
Resting and sensitized T lymphocytes exhibit distinct stimulatory (antigen-presenting cell) requirements for growth and...
Previous studies have shown that unprimed or resting T lymphocytes will grow and release lymphokines when stimulated by dendritic cells (DC). We now have examined the stimulatory requirements for antigen-primed or blast-transformed T cells. The latter were derived from dendritic/T cell clusters that developed during the primary mixed leukocyte reaction (MLR). The specificity of the blasts was established by a binding assay in which most T cells aggregated small B lymphocytes of the appropriate haplotype within 2 h at 4 or 37 degrees C. Since unprimed T cells did not aggregate allogeneic B cells, we suggest that DC induce T lymphocytes to express additional functioning receptors for antigen. Lyt-2-T blasts did not grow or release interleukin 2 or B cell helper factors unless rechallenged with specific alloantigen, whereupon growth (generation time of 14-18 h) and lymphokine release rapidly resumed. The blasts could be stimulated by allogeneic ...http://jem.rupress.org/content/160/6/1717
Role of the pineal gland in immunity. Circadian synthesis and release of melatonin modulates the antibody response and...
Inhibition of synthesis of the pineal neurohormone melatonin (MEL) in mice, by administration of propranolol (PRO) in the evening, and daily injections of p-chlorophenylalanine (PCPA), resulted in a significant depression of the primary antibody response to sheep red blood cells (SRBC). Spleen cells from these mice showed a reduced reactivity against antigens in the autologous mixed lymphocyte reaction (AMLR). In contrast, alloreactivity remained normal. Reconstitution of the night-time peak of plasma MEL by evening injections to the mice completely reversed the suppression of the humoral response and the AMLR. MEL administration was able to antagonize the depression of antibody production induced by corticosterone in vivo. These results suggest that the pineal gland has important immunomodulatory functions through its cyclic, circadian release of MEL.https://www.semanticscholar.org/paper/Role-of-the-pineal-gland-in-immunity-Circadian-syn-Maestroni-Conti/448615c8c08e0ea32204ad22baf8a1f4cebc1484
Human T-cell clones from autoimmune thyroid glands: specific recognition of autologous thyroid cells. - The Kennedy Institute...
The thyroid glands of patients with autoimmune diseases such as Graves' disease and certain forms of goiter contain infiltrating activated T lymphocytes and, unlike cells of normal glands, the epithelial follicular cells strongly express histocompatibility antigens of the HLA-DR type. In a study of such autoimmune disorders, the infiltrating T cells from the thyroid glands of two patients with Graves' disease were cloned in mitogen-free interleukin-2 (T-cell growth factor). The clones were expanded and their specificity was tested. Three types of clones were found. One group, of T4 phenotype, specifically recognized autologous thyroid cells. Another, also of T4 phenotype, recognized autologous thyroid or blood cells and thus responded positively in the autologous mixed lymphocyte reaction. Other clones derived from cells that were activated in vivo were of no known specificity. These clones provide a model ...https://www.kennedy.ox.ac.uk/publications/481940
"The effect of thiabendazole in a mixed leukocyte culture." by E J. Lovett and J Lundy
Lovett, E J. and Lundy, J, "The effect of thiabendazole in a mixed leukocyte culture." (1977). Subject Strain Bibliography 1977. 1493 ...https://mouseion.jax.org/ssbb1977/1493/
AID 132509 - Compound was tested in vitro for immunosuppressive activity which was measured in the mouse mixed lymphocyte...
BioAssay record AID 132509 submitted by ChEMBL: Compound was tested in vitro for immunosuppressive activity which was measured in the mouse mixed lymphocyte reaction.https://pubchem.ncbi.nlm.nih.gov/bioassay/132509
Antigen specificity of mixed lymphocyte response-induced suppr...
Antigen specificity of mixed lymphocyte response-induced suppressor cells.: The nature of the antigens recognized by mixed lymphocyte response-generated suppreshttps://www.mysciencework.com/publication/show/antigen-specificity-mixed-lymphocyte-response-induced-suppressor-cells-7f7318f3
CONSEQUENT INTRAFAMILIAL IMMUNIZATION FOR DL-A HAPLOTYPING IN CANINES | JEM
A procedure of intrafamilial immunization is described for production of antisera recognizing DL-A haplotypes. In a colony consisting of 1 sire, 6 bitches, and 67 offspring all haplotypes could be accurately allocated. In the colony the observed reaction frequencies of the antisera are in agreement with mendelian codominant inheritance. Mixed lymphocyte culture tests confirmed the accuracy of the serologic typing and the presence of homozygous individuals within the colony. Further evidence is presented supporting the presence of two or more subloci within the DL-A system. Colonies of canines such as the one described should provide a sensitive system for evaluating interaction between serologic DL-A typing, MLC reactivity, and immune response genetics in a nonrodent species which is not highly inbred.. ...http://jem.rupress.org/content/137/2/494
Allostimulatory and processing capacities of D1 cells. | Open-i
Allostimulatory and processing capacities of D1 cells. (a) Mixed lymphocyte reaction (MLR) by sorted I-Abright and I-Aint D1 cells shows that the mature I-https://openi.nlm.nih.gov/detailedresult.php?img=PMC2196118_JEM.winzler8&req=4
Coupled complementation of immune response genes controlling responsiveness to the H-2.2 alloantigen. | JEM
The ability of various B10 congenic resistant strains to respond to the alloantigen H-2.2 was tested. High and low antibody-producing strains were distinguished by their anti-H-2.2 hemagglutinating respones. However, these strains do not differ in their ability to respond to these antigenic differences in the mixed lymphocyte culture. The humoral response to the H-2.2 alloantigen was shown to be controlled by two interacting genes localized within the H-2 complex. Thus, F1 hybrids prepared between parental low responder strains could yield high level immune responses. In addition, strains bearing recombinant H-2 haplotypes were used to map the two distinct genes controlling the immune response. The alleles at each locus were shown to be highly polymorphic as evidenced by the asymmetric complementation patterns observed. The restricted interactions of specific alleles was termed coupled complementation. The significance of ...http://jem.rupress.org/content/146/2/571
ESCRS | European Society of Cataract & Refractive Surgeons
Five Chinese rhesus macaques which received decellularized porcine corneal lamellae and were followed up for at least 6 months underwent full-thickness corneal allotransplantation. Previous xeno-grafts (7.5mm in diameter) were removed with 8.0mm trephines and 8.5mm-sized grafts from donor macaques were transplanted. Clinically applicable minimal immunosuppression was done based on topical, subconjunctival and systemic corticosteroids. Rejection signs and serial changes in recipients blood profile, including memory T cell subset, anti-Gal and donor pig specific antibodies, and complement were evaluated. Changes in aqueous complement concentration were also assessed at 4 weeks after transplantation. The mixed lymphocytes reaction (MLR) was analyzed in three rhesus macaques (KPed rhesus) with clear allografts that survived more than 6 months. MLR was done using peripheral blood mononuclear cells (PBMC). PBMCs from the recipient rhesus and normal rhesus were used as responder ...http://www.escrs.org/milan2012/programme/poster-details.asp?id=13122
In Vivo Effects of Calcitriol on Phenotypic and Functional Properties of Dendritic Cells
Background: Dendritic cells (DCs) play a central role in the initiation and expansion of T cell mediated immune responses with potential immunotherapy application. The compounds which have the ability to induce immunomodulatory effects on DCs may be employed for the treatment of immunopathologic conditions such as autoimmune diseases. Objective: The aim of this study was to investigate the in vivo effects of calcitriol (active form of vitamin D3) on DCs. Methods: 0.1 microgram calcitriol was injected intra-peritoneally into C57BL/6 mice every other day within 3 weeks, and spleen DCs were extracted by magnetic beads. The phenotypic and functional properties of DCs were studied by flow cytometry and mixed lymphocyte reaction (MLR), respectively. Results: The expression of CD86 and MHC II, as maturation markers and costimulatory molecules were significantly decreased (p=0.028 and p=0.047, respectively) while CD11b expression, as a marker of mice myeloid DCs which mostly induces ...http://iji.sums.ac.ir/article_17042.html
Modulatory role of regulatory T cells in a murine model of severe equine asthma | BMC Veterinary Research | Full Text
In this paper, we describe that the transient decrease of CD4+, CD25+, Foxp3+ splenic T regulatory cells, induced by Cy, correlates with increased airway inflammation in mice exposed to hay contaminated with Aspergillus fumigatus.. The results presented here demonstrate that, in our experimental model, older animals (8 months old) showed a higher percentage of CD4+, CD25+, Foxp3+ cells in the spleen when compared to younger mice (2 months old). Similar findings were reported by Zhao , who also compared the capacity of CD4+, CD25+ cells from older and younger mice to inhibit delayed hypersensitivity, mixed lymphocyte reaction or cytokine production. From their results, they concluded that CD4+, CD25+ cells present in the older mice, were less effective in suppressing the above-mentioned assays. These facts may explain why aging produces a progressive decline in immune regulation, and in the case of horses and in the mice of our experimental model , why age facilitates ...https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-017-1037-0
FR-900520 and FR-900523, novel immunosuppressants isolated from a Streptomyces. II. Fermentation, isolation and physico...
FR-900520 and FR-900523, novel neutral macrolide immunosuppressants, were isolated from the cultured broth of Streptomyces hygroscopicus subsp. yakushimaensis No. 7238. Their molecular formulae were determined as C43H69NO12 and C42H67NO12, respectively. The compounds suppressed immune response in vitro. IC50 values of FR-900520 and FR-900523 for mouse mixed lymphocyte reaction were 0.55 nM and 1.6 nM, respectively. FR-900520, the major component, clearly prolonged skin allograft survival in rats.https://www.semanticscholar.org/paper/FR-900520-and-FR-900523-novel-immunosuppressants-i-Hatanaka-Kino/2eb22b4cfc95fedcdeb172a527a2455032c16284
"Cytotoxicity of allogeneic lymphocytes sensitized against h-2 antigens" by W Ax, H S. Koren et al.
Ax, W; Koren, H S.; and Fischer, H, "Cytotoxicity of allogeneic lymphocytes sensitized against h-2 antigens in vitro." (1971). Subject Strain Bibliography 1971. 191 ...http://mouseion.jax.org/ssbb1971/191/
Transplant of Epithelium Conjunctival Human Autologous Cultivated ex Vivo in Amniotic Membrane for the Treatment of...
The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details ...https://clinicaltrials.gov/ct2/show/results/NCT00799526
"Phytohemagglutinin stimulated lymphocyte cultures: distribution and do" by James G. Krueger
Krueger, James G., "Phytohemagglutinin stimulated lymphocyte cultures: distribution and dose-response levels in ten strains of mice." (1974). Summer and Academic Year Student Reports. 1328 ...http://mouseion.jax.org/strp/1328/
"Effects of cell-bound phytohemagglutinin and concanavalin a on the mix" by J R. Schmidtke, P K. Ray et al.
Schmidtke, J R.; Ray, P K.; and Simmons, R L., "Effects of cell-bound phytohemagglutinin and concanavalin a on the mixed leucocyte response of mouse spleen cells in vitro." (1974). Subject Strain Bibliography 1974. 1861 ...https://mouseion.jax.org/ssbb1974/1861/