Cellulose and lignin colocalization at the plant cell wall surface limits microbial hydrolysis of Populus biomass - Green...
However, non-pretreated woody biomass has shown high resistance to hydrolysis by cellulolytic microbes or purified cellulases. ... We investigate the limited solubilization of Populus deltoides by the cellulolytic thermophile Clostridium thermocellum in the ... This confirmed that biomass surfaces had become non-productive for the C. thermocellum hydrolytic exoproteome. This study ... However, non-pretreated woody biomass has shown high resistance to hydrolysis by cellulolytic microbes or purified cellulases. ...http://pubs.rsc.org/en/content/articlelanding/2017/gc/c7gc00346c
The US-DOE Bioenergy Science Center (BESC): Findings and Perspectives
Clostridium thermocellum is a promising microorganism for conversion of cellulosic biomass to biofuel, without added enzymes; ... and high biomass loadings (Verbeke et al.). There are also examples of metabolic engineering (Zheng et al.; Eminoglu et al.) as ... Expression of adhA from different organisms in Clostridium thermocellum Clostridium thermocellum is a cellulolytic anaerobic ... Clostridium thermocellum and Thermoanaerobacterium saccharolyticum are prominent candidate biocatalysts that, together, can ...https://www.biomedcentral.com/collections/besc
Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review
Moreover, high-throughput techniques, such as metagenomics, metatranscriptomics, next generation sequencing and synthetic ... it is essential to bring into attention all barriers faced and take advantage of all the experiences gained to achieve a high- ... production from lignocellulosic and starchy biomass. The CBP strategies include using native single strains with cellulytic and ... biology developed to explore novel microorganisms and powerful enzymes with high activity, thermostability and pH stability are ...https://www.biofueljournal.com/article_8440.html
Caldicellulosiruptor bescii - Wikipedia
This genetically modified strain is able to convert biomass composed of switch-grass to ethanol. C. bescii has the highest ... Bi-functional acetaldehyde/alcohol dehydrogenase genes from Clostridium thermocellum allow for the conversion of sugars to ... "Metabolic engineering of Caldicellulosiruptor bescii yields increased hydrogen production from lignocellulosic biomass". ...https://en.wikipedia.org/wiki/Caldicellulosiruptor_bescii
Clostridium thermocellum - Wikipedia
This makes it useful in converting biomass into a usable energy source. The degradation of the cellulose is carried out in the ... The cellulase system of the bacterium significantly differs from fungal cellulases due to its high activity on crystalline ... JGI: Clostridium thermocellum ATCC 27405 Cellulase, Clostridia, and Ethanol Ibid. Characterization of Clostridium thermocellum ... Clostridium thermocellum is an anaerobic, thermophilic bacterium. C. thermocellum has garnered research interest due to its ...https://en.wikipedia.org/wiki/Clostridium_thermocellum
Cellulose utilization by Clostridium thermocellum: Bioenergetics and hydrolysis product assimilation | PNAS
Office of the Biomass Program of U.S. Department of Energy (2003) Biomass Program: Multi-Year Technical Plan FY04-FY08, www. ... thermocellum cellulase system are higher molecular weight cellodextrins that are rapidly cleaved in the absence of cells and ... Cellulose utilization by Clostridium thermocellum: Bioenergetics and hydrolysis product assimilation Message Subject (Your Name ... Cellulose utilization by Clostridium thermocellum: Bioenergetics and hydrolysis product assimilation. Yi-Heng Percival Zhang, ...https://www.pnas.org/content/102/20/7321?ijkey=82a2ddbbb3b723f8592c4bdd61ed25f53aa9bba4&keytype2=tf_ipsecsha
Cellulosome - Wikipedia
Clostridium cellulovorans Clostridium clariflavum Clostridium josui Clostridium papyrosolvens Clostridium thermocellum (treated ... The high-affinity cohesin-dockerin interaction defines the cellulosome structure. Attachment of the cellulosome to its ... of cellulosomal domains should enable better use of cellulosic biomass and may offer a wide range of novel applications. In the ... The scaffoldin of some cellulosomes, an example being that of Clostridium thermocellum, contains a carbohydrate-binding module ...https://en.wikipedia.org/wiki/Cellulosome
Technology -Genetic Engineered Clostridium Thermocellum for Ethanol Production from Cellulose
The ideal organism to make ethanol from cellulose biomass will break down cellulose, ferment sugar, tolerate high ... The anaerobic bacterium Clostridium thermocellum is an excellent candidate for conversion of renewable cellulose biomass to ... C. thermocellum uses a large number of enzymes to break down a range of biomass types, and a mechanism to selectively modulate ... Clostridium thermocellum is a thermophile with cellulase genes. It is an ideal candidate for these genetic manipulations. ...http://rochester.technologypublisher.com/technology/2966
The BioEnergy Science Center: NREL explains the higher cellulolytic activity of a vital microorganism
... thermocellum on biomass. A paper reporting the potential for the bacterium, "Dramatic performance of Clostridium Thermocellum ... Their discovery was made during an investigation into the performance of Clostridium thermocellum. The scientists found the ...http://bioenergysciencecenter.blogspot.com/2016/02/nrel-explains-higher-cellulolytic.html
Frontiers | Comparative feedstock analysis in Setaria viridis L. as a model for C4 bioenergy grasses and Panicoid crop species ...
... material are examined to compare biomass performance metrics. ... obtained through Clostridium thermocellum system during biomass ... was the only case where the root biomass conversion products were higher than the conversion products from the shoot biomass. ... Clostridium thermocellum ATCC 27405 was provided by Michael Flythe (USDA, Lexington, KY, USA) and was maintained in our ... used a cellulosome digestion via Clostridium thermocellum and measured the output of carbon from raw unprocessed biomass. ...https://www.frontiersin.org/articles/10.3389/fpls.2013.00181/full
Novel perspectives for evolving enzyme cocktails for lignocellulose hydrolysis in biorefineries | Sustainable Chemical...
Significant potential exists for bioconversion of biomass, the most abundant and also the most renewable biomaterial on our ... The current scenario, however, also warrants the need for research and development of integrated biomass production and ... efforts to utilize renewable resources for the production of greener energy and a replacement which can also meet the high ... the cellulolytic anaerobes such as Clostridium thermocellum and Bacteroides cellulosolvens produce cellulases with high ...https://sustainablechemicalprocesses.springeropen.com/articles/10.1186/2043-7129-1-15
Integrated analysis of hydrothermal flow through pretreatment | Biotechnology for Biofuels | Full Text
Compared to SSF, solubilization by C. thermocellum was 30% higher after 4 days, and was over twice as fast on ball-milled FT- ... FT pretreatment consistently resulted in higher XMG recovery, higher removal of non-carbohydrate carbon and higher glucan ... Corn stover exhibited higher hydrolysis yields than bagasse or poplar, which could be due to higher removal of non-carbohydrate ... Removal of non-carbohydrate carbon during FT pretreatment increased from 65% at low severity to 80% at high severity for corn ...https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/1754-6834-5-49
New Discovery Could Improve Ethanol Production - CleanTechies
"Clostridium thermocellum". With it scientists will be able to experiment with genetically altering biomass plants to produce ... but scientists so far had been unable to determine the genetic basis for its ability to tolerate higher concentrations of ... The Clostridium thermocellum has been studied for decades, ... Biomassclean energydepartment of energyenzymesethanol ... Current methods to make ethanol from a type of biomass found in switchgrass and agricultural waste require the addition of ...http://cleantechies.com/2011/08/15/new-discovery-could-improve-ethanol-production/
Recent Bachelor's Research Profile - Malaney Abel | Science Education Programs at ORNL
... a participant in the Higher Education Research Experiences (HERE) Recent Bachelor's program at Oak Ridge National Laboratory, ... Adam Guss, Abel is studying the bacterium Clostridium thermocellum used to produce cellulosic ethanol, an alternative fuel ... "Being able to use cellulose in ethanol production will aide in maximizing biomass efficiency," explained Abel. "This, in turn, ... Malaney Abel, a participant in the Higher Education Research Experiences (HERE) Recent Bachelor's program at Oak Ridge National ...https://www.orau.org/ornl/recent-bachelors-associates/profile-malaney-abel.htm
Molecular and Biochemical Analyses of CbCel9A/Cel48A, a Highly Secreted Multi-Modular Cellulase by Caldicellulosiruptor bescii...
High activity CAZyme cassette for improving biomass degradation in thermophiles. *Roman Brunecky, Daehwan Chung, +11 authors ... Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum. *Ya-Jun Liu, Shiyue Liu, Sheng ... CelI, a noncellulosomal family 9 enzyme from Clostridium thermocellum, is a processive endoglucanase that degrades crystalline ... Supplementing with Non-Glycoside Hydrolase Proteins Enhances Enzymatic Deconstruction of Plant Biomass. *Xiaoyun Su, Jing Zhang ...https://www.semanticscholar.org/paper/Molecular-and-Biochemical-Analyses-of-a-Highly-by-Yi-Su/9ba5e8b8445ac653c5029bcf66615e760ad365c5
Single, key gene discovery could streamline production of... ( WASHINGTON DC -- A team of researche...)
Although scientists have studied Clostridium thermocellum for decades...,Single,,key,gene,discovery,could,streamline,production ... Although scientists have studied Clostridium thermocellum for decades, the genetic basis for its ability to tolerate higher ... "Clostridium thermocellum" will mean that scientists can now experiment with genetically altering biomass plants to produce more ... This discovery could be the missing link in developing biomass crops that produce higher concentrations of ethanol at lower ...http://www.bio-medicine.org/biology-news-1/Single--key-gene-discovery-could-streamline-production-of-biofuels--20819-1/
Expression of a heat-stable NADPH-dependent alcohol dehydrogenase from Thermoanaerobacter pseudethanolicus 39E in Clostridium...
09 BIOMASS FUELS; consolidated bioprocessing; Clostridium thermocellum; butanol dehydrogenase; furfural; 5-hydroxymethyl-2- ... Redirecting carbon flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum journal, ... Clostridium thermocellum is a promising candidate for ethanol production from cellulosic biomass, but requires metabolic ... Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation Journal Article Sander, Kyle B. ; Wilson, ...https://www.osti.gov/pages/biblio/1350020-expression-heat-stable-nadph-dependent-alcohol-dehydrogenase-from-thermoanaerobacter-pseudethanolicus-clostridium-thermocellum-results-increased-hydroxymethylfurfural-resistance
Characterization ofClostridium thermocellumstrains with disrupted fermentation end-product pathways | SpringerLink
Clostridium thermocellum is a thermophilic, cellulolytic anaerobe that is a candidate microorganism for industrial biofuels ... Clostridium thermocellum and Clostridium stercorarium, thermophilic plant-fiber degraders. Ann N Y Acad Sci 1125:298-307PubMed ... Free amino acids were secreted by all examined strains, with both Δpta strains secreting higher amounts of alanine, valine, ... Cellobiose-grown cultures of the Δldh strain had identical biomass accumulation, fermentation end-products, transcription ...https://link.springer.com/article/10.1007%2Fs10295-013-1275-5
Frontiers | Glucose-tolerant β-glucosidase retrieved from a Kusaya gravy metagenome | Microbiology
It had a low KM (78 µM with p-nitrophenyl β-D-glucoside; 0.36 mM with cellobiose) and high Vmax (91 µmol min-1 mg-1 with p- ... hydrolyze cellooligosaccharides to glucose and play a crucial role in the enzymatic saccharification of cellulosic biomass. ... hydrolyze cellooligosaccharides to glucose and play a crucial role in the enzymatic saccharification of cellulosic biomass. ... Addition of cloned β-glucosidase enhances the degradation of crystalline cellulose by Clostridium thermocellum cellulase ...https://www.frontiersin.org/articles/10.3389/fmicb.2015.00548/full
Winter rye as a bioenergy feedstock: impact of crop maturity on composition, biological solubilization and potential revenue |...
... thermocellum achieved total solubilization 33% higher than that of SSCF for the earliest harvest date and 50% higher for the ... Winter rye was ground and autoclaved then fermented at 15 g/L total solids by either (1) Clostridium thermocellum or (2) ... This study investigates the dynamic trends in biomass yield, composition, and biological solubilization over the course of a ... thermocellum fermentation and SSCF. C. thermocellum fermentation achieved higher carbohydrate solubilization than SSCF across ...https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-015-0225-z
Metabolome analysis reveals a role for glyceraldehyde 3-phosphate dehydrogenase in the inhibition of C. thermocellum by ethanol...
For C. thermocellum, we observed that ethanol tolerance, in experiments with gradual ethanol addition, was twofold higher than ... Our hypothesis is supported by enzyme assays showing greater sensitivity of the C. thermocellum enzyme to high levels of NADH, ... thermocellum is inhibited by high levels of ethanol. We then showed that this bottleneck could be relieved by expression of the ... and by the increase in ethanol tolerance and production when the T. saccharolyticum gapdh was expressed in C. thermocellum. We ...https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-017-0961-3
Biomass and Biofuels Technologies - Energy Innovation Portal
Clostridium Thermocellum CbhA- Amino acid sequence modified for enhanced catalytic activity in the saccharification of ... High-Yielding Method for Converting Biomass to Fermentable Sugars for Biofuel Production Lignocellulosic biomass is a very ... One- and Two-Phase Conversion of Biomass to Furfural Exploiting the energy potential of biomass high in cellulose and lignin- ... High Starch in Plant Leaves at Senescence Currently, there is a great interest in using plant biomass, instead of grain, to ...http://techportal.eere.energy.gov/category.do/categoryID=1
Metabolic Engineering of Clostridium cellulolyticum for Production of Isobutanol from Cellulose | Applied and Environmental...
... cellulolytic Clostridium thermocellum. This strain is particularly attractive because it is able to thrive in high-temperature ... Progress and challenges in enzyme development for biomass utilization. Adv. Biochem. Eng. Biotechnol. 108:95-120. ... Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant. ... Proteomic profile changes in membranes of ethanol-tolerant Clostridium thermocellum. Appl. Microbiol. Biotechnol. 74:422-432. ...https://aem.asm.org/content/77/8/2727
Single, Key Gene Discovery Could Streamline Production Of Biofuels
Although scientists have studied Clostridium thermocellum for decades, the genetic basis for its ability to tolerate higher ... "Clostridium thermocellum" will mean that scientists can now experiment with genetically altering biomass plants to produce more ... This discovery could be the missing link in developing biomass crops that produce higher concentrations of ethanol at lower ... Current methods to make ethanol from a type of biomass found in switchgrass and agricultural waste require the addition of ...https://www.technologynetworks.com/genomics/news/single-key-gene-discovery-could-streamline-production-of-biofuels-209570
Transcriptomic and proteomic analyses of core metabolism in Clostridium termitidis CT1112 during growth on α-cellulose, xylan,...
The majority of the proteins associated with central metabolism were detected in high abundance. While major differences were ... and provides a foundation for future studies on metabolic engineering to optimize biofuel production from natural biomass. ... Clostridium termitidis CT1112 is an anaerobic, Gram-positive, mesophilic, spore-forming, cellulolytic bacterium, originally ... Impact of pretreated Switchgrass and biomass carbohydrates on Clostridium thermocellum ATCC 27405 cellulosome composition: a ...https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-016-0711-x