*  Cellulose and lignin colocalization at the plant cell wall surface limits microbial hydrolysis of Populus biomass - Green...
However, non-pretreated woody biomass has shown high resistance to hydrolysis by cellulolytic microbes or purified cellulases. ... We investigate the limited solubilization of Populus deltoides by the cellulolytic thermophile Clostridium thermocellum in the ... This confirmed that biomass surfaces had become non-productive for the C. thermocellum hydrolytic exoproteome. This study ... However, non-pretreated woody biomass has shown high resistance to hydrolysis by cellulolytic microbes or purified cellulases. ...
  http://pubs.rsc.org/en/content/articlelanding/2017/gc/c7gc00346c
*  The US-DOE Bioenergy Science Center (BESC): Findings and Perspectives
Clostridium thermocellum is a promising microorganism for conversion of cellulosic biomass to biofuel, without added enzymes; ... and high biomass loadings (Verbeke et al.). There are also examples of metabolic engineering (Zheng et al.; Eminoglu et al.) as ... Expression of adhA from different organisms in Clostridium thermocellum Clostridium thermocellum is a cellulolytic anaerobic ... Clostridium thermocellum and Thermoanaerobacterium saccharolyticum are prominent candidate biocatalysts that, together, can ...
  https://www.biomedcentral.com/collections/besc
*  Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review
Moreover, high-throughput techniques, such as metagenomics, metatranscriptomics, next generation sequencing and synthetic ... it is essential to bring into attention all barriers faced and take advantage of all the experiences gained to achieve a high- ... production from lignocellulosic and starchy biomass. The CBP strategies include using native single strains with cellulytic and ... biology developed to explore novel microorganisms and powerful enzymes with high activity, thermostability and pH stability are ...
  https://www.biofueljournal.com/article_8440.html
*  Caldicellulosiruptor bescii - Wikipedia
This genetically modified strain is able to convert biomass composed of switch-grass to ethanol. C. bescii has the highest ... Bi-functional acetaldehyde/alcohol dehydrogenase genes from Clostridium thermocellum allow for the conversion of sugars to ... "Metabolic engineering of Caldicellulosiruptor bescii yields increased hydrogen production from lignocellulosic biomass". ...
  https://en.wikipedia.org/wiki/Caldicellulosiruptor_bescii
*  Clostridium thermocellum - Wikipedia
This makes it useful in converting biomass into a usable energy source. The degradation of the cellulose is carried out in the ... The cellulase system of the bacterium significantly differs from fungal cellulases due to its high activity on crystalline ... JGI: Clostridium thermocellum ATCC 27405 Cellulase, Clostridia, and Ethanol Ibid. Characterization of Clostridium thermocellum ... Clostridium thermocellum is an anaerobic, thermophilic bacterium. C. thermocellum has garnered research interest due to its ...
  https://en.wikipedia.org/wiki/Clostridium_thermocellum
*  Cellulosome - Wikipedia
Clostridium cellulovorans Clostridium clariflavum Clostridium josui Clostridium papyrosolvens Clostridium thermocellum (treated ... The high-affinity cohesin-dockerin interaction defines the cellulosome structure. Attachment of the cellulosome to its ... of cellulosomal domains should enable better use of cellulosic biomass and may offer a wide range of novel applications. In the ... The scaffoldin of some cellulosomes, an example being that of Clostridium thermocellum, contains a carbohydrate-binding module ...
  https://en.wikipedia.org/wiki/Cellulosome
*  The BioEnergy Science Center: NREL explains the higher cellulolytic activity of a vital microorganism
... thermocellum on biomass. A paper reporting the potential for the bacterium, "Dramatic performance of Clostridium Thermocellum ... Their discovery was made during an investigation into the performance of Clostridium thermocellum. The scientists found the ...
  http://bioenergysciencecenter.blogspot.com/2016/02/nrel-explains-higher-cellulolytic.html
*  Frontiers | Comparative feedstock analysis in Setaria viridis L. as a model for C4 bioenergy grasses and Panicoid crop species ...
... material are examined to compare biomass performance metrics. ... obtained through Clostridium thermocellum system during biomass ... was the only case where the root biomass conversion products were higher than the conversion products from the shoot biomass. ... Clostridium thermocellum ATCC 27405 was provided by Michael Flythe (USDA, Lexington, KY, USA) and was maintained in our ... used a cellulosome digestion via Clostridium thermocellum and measured the output of carbon from raw unprocessed biomass. ...
  https://www.frontiersin.org/articles/10.3389/fpls.2013.00181/full
*  New Discovery Could Improve Ethanol Production - CleanTechies
"Clostridium thermocellum". With it scientists will be able to experiment with genetically altering biomass plants to produce ... but scientists so far had been unable to determine the genetic basis for its ability to tolerate higher concentrations of ... The Clostridium thermocellum has been studied for decades, ... Biomassclean energydepartment of energyenzymesethanol ... Current methods to make ethanol from a type of biomass found in switchgrass and agricultural waste require the addition of ...
  http://cleantechies.com/2011/08/15/new-discovery-could-improve-ethanol-production/
*  Recent Bachelor's Research Profile - Malaney Abel | Science Education Programs at ORNL
... a participant in the Higher Education Research Experiences (HERE) Recent Bachelor's program at Oak Ridge National Laboratory, ... Adam Guss, Abel is studying the bacterium Clostridium thermocellum used to produce cellulosic ethanol, an alternative fuel ... "Being able to use cellulose in ethanol production will aide in maximizing biomass efficiency," explained Abel. "This, in turn, ... Malaney Abel, a participant in the Higher Education Research Experiences (HERE) Recent Bachelor's program at Oak Ridge National ...
  https://www.orau.org/ornl/recent-bachelors-associates/profile-malaney-abel.htm
*  Single, key gene discovery could streamline production of... ( WASHINGTON DC -- A team of researche...)
Although scientists have studied Clostridium thermocellum for decades...,Single,,key,gene,discovery,could,streamline,production ... Although scientists have studied Clostridium thermocellum for decades, the genetic basis for its ability to tolerate higher ... "Clostridium thermocellum" will mean that scientists can now experiment with genetically altering biomass plants to produce more ... This discovery could be the missing link in developing biomass crops that produce higher concentrations of ethanol at lower ...
  http://www.bio-medicine.org/biology-news-1/Single--key-gene-discovery-could-streamline-production-of-biofuels--20819-1/
*  Characterization ofClostridium thermocellumstrains with disrupted fermentation end-product pathways | SpringerLink
Clostridium thermocellum is a thermophilic, cellulolytic anaerobe that is a candidate microorganism for industrial biofuels ... Clostridium thermocellum and Clostridium stercorarium, thermophilic plant-fiber degraders. Ann N Y Acad Sci 1125:298-307PubMed ... Free amino acids were secreted by all examined strains, with both Δpta strains secreting higher amounts of alanine, valine, ... Cellobiose-grown cultures of the Δldh strain had identical biomass accumulation, fermentation end-products, transcription ...
  https://link.springer.com/article/10.1007%2Fs10295-013-1275-5
*  Frontiers | Glucose-tolerant β-glucosidase retrieved from a Kusaya gravy metagenome | Microbiology
It had a low KM (78 µM with p-nitrophenyl β-D-glucoside; 0.36 mM with cellobiose) and high Vmax (91 µmol min-1 mg-1 with p- ... hydrolyze cellooligosaccharides to glucose and play a crucial role in the enzymatic saccharification of cellulosic biomass. ... hydrolyze cellooligosaccharides to glucose and play a crucial role in the enzymatic saccharification of cellulosic biomass. ... Addition of cloned β-glucosidase enhances the degradation of crystalline cellulose by Clostridium thermocellum cellulase ...
  https://www.frontiersin.org/articles/10.3389/fmicb.2015.00548/full
*  Biomass and Biofuels Technologies - Energy Innovation Portal
Clostridium Thermocellum CbhA- Amino acid sequence modified for enhanced catalytic activity in the saccharification of ... High-Yielding Method for Converting Biomass to Fermentable Sugars for Biofuel Production Lignocellulosic biomass is a very ... One- and Two-Phase Conversion of Biomass to Furfural Exploiting the energy potential of biomass high in cellulose and lignin- ... High Starch in Plant Leaves at Senescence Currently, there is a great interest in using plant biomass, instead of grain, to ...
  http://techportal.eere.energy.gov/category.do/categoryID=1
*  Untitled Document
06/27/2017: Our paper on 'Linking Overflow Metabolism and Growth Cessation in Clostridium thermocellum DSM1313 during High ... Elucidating Clostridium thermocellum Central Metabolism For Enhanced Ethanol Production From Lignocellulosic Biomass' and ' ... Trinh attended BESC 2013 retreat in Chantanooga, TN and presented a poster on: 'Characterization of Clostridium thermocellum ... Reconstruction of Predictive Genome-Scale Metabolic Networks of Clostridium thermocellum DSM 1313' ...
  http://web.utk.edu/~ctrinh/news.html
*  BioEnergy Science Center
Microbial solubilization of biomass selectively targets carbohydrates. Clostridium thermocellum, a highly effective ... Findings Could Lead to More Efficient Ways of Breeding High-Ethanol-Yielding Poplar and Other Tree Species. Using whole-genome ... Engineering T. saccharolyticum Ethanol Pathway into C. thermocellum Improves Biofuel Production. Clostridium thermocellum is a ... BESC researchers found that naturally cellulolytic microbes such as Clostridium thermocellum (stained green and growing on ...
  http://bioenergycenter.org/besc/
*  Project : USDA ARS
... we have developed a simultaneous saccharification and fermentation assay based on the anaerobe Clostridium thermocellum. ... ARS scientists in Albany, CA and collaborators at CSIRO in Canberra, Australia have begun to use a high-throughput phenotyping ... Identify induced and natural variation in traits relevant to biomass crop improvement using the model grass Brachypodium. ... Chromosome arm length ratios and sizes have identified with high reliability the other chromosomes which are not yet aligned ...
  https://www.ars.usda.gov/research/project/?accnNo=418493&fy=2010
*  Heterologous expression of family 10 xylanases from Acidothermus cellulolyticus enhances the exoproteome of...
pSKW10 or pSKW11 also contain a wild-type pyrF allele from Clostridium thermocellum and were transformed into JWCB52 that ... Acidothermus cellulolyticus contain CAZymes with much simpler architectures that exhibit high specific activity on biomass at ... Clostridium thermocellum [35, 36], and Escherichia coli [37, 38], however, cannot efficiently utilize C5 sugars. C. bescii ... Growth of the JWCB74 and JWCB75 on birchwood xylan was 30.5- (P value = 0.013) and 26.0-fold (P value = 0.023) higher than that ...
  https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-016-0588-9
*  Metabolic control ofClostridium thermocellumvia inhibition of hydrogenase activity and the glucose transport rate | SpringerLink
Clostridium thermocellum has the ability to catabolize cellulosic biomass into ethanol, but acetic acid, lactic acid, carbon ... Clostridium thermocellum has the ability to catabolize cellulosic biomass into ethanol, but acetic acid, lactic acid, carbon ... This research highlights the complex effects of high concentrations of dissolved gases in fermentation, which are increasingly ... Özkan M, Yilmaz EI, Lynd LR, Özcengiz G (2004) Cloning and expression of the Clostridium thermocellum l-lactate dehydrogenase ...
  https://link.springer.com/article/10.1007%2Fs00253-011-3812-3
*  Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β-glucosidase | Microbial Cell...
Cellulosic biomass is considered as a promising alternative to fossil fuels, but its recalcitrant nature and high cost of ... Berger E, Zhang D, Zverlov VV, Schwarz WH: Two noncellulosomal cellulases of Clostridium thermocellum, Cel9I and Cel48Y, ... recalcitrant nature of cellulosic biomass and the high cost of cellulase are the major obstacles to utilize cellulosic biomass ... containing a single type of cohesin and dockerin pair from Clostridium thermocellum, were co-expressed in a single strain, ...
  https://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-12-14
*  Isolation and Characterization of Bacteria That Use Furans as the Sole Carbon Source | SpringerLink
Freier, D., Mothershed, C. P., & Wiegel, J. (1988). Characterization of Clostridium thermocellum JW20. Applied and ... This strain also had the greatest specific growth rate on furfural (0.6/h at 27-30 °C) and showed the highest specific activity ... Nichols, N. N., Dien, B. S., Guisado, G. M., & López, M. J. (2005). Bioabatement to remove inhibitors form biomass-derived ... Inhibition of ethanol-producing yeast and bacteria by degradation products produced during pre-treatment of biomass. Applied ...
  https://link.springer.com/article/10.1007/s12010-015-1859-9
*  NAVER Academic | Expression of alginases and alginate polymerase genes in response to oxygen, and their relationship with the...
Conserved reiterated domains in Clostridium thermocellum endoglucanases are not essential for catalytic activity (1988) Hall ... High quality biodiesel production from a microalga Chlorella protothecoides by heterotrophic growth in fermenters (2006) Xu Han ... Comparison of dilute acid and ionic liquid pretreatment of switchgrass: Biomass recalcitrance, delignification and enzymatic ...
  http://academic.naver.com/article.naver?doc_id=64815234
*  Organizer Protein Marshals Bacterial Forces Against Cellulose : Rochester News
Clostridium thermocellum is a bacterium with a remarkable combination of desirable qualities,' says Demain. Also working on the ... so that we'd have both a high volume and a high efficiency of cellulose degradation in one organism.' The discovery was made in ... Though biomass offers an abundant source of energy, and is much cleaner than conventional fossil fuels, it remains largely ... It's also an anaerobic bacterium that grows at high temperatures and thrives without oxygen; maintaining an adequate supply of ...
  https://www.rochester.edu/news/show.php?id=130
*  WikiGenes - PERCHLORIC ACID - perchloric acid
31P NMR studies of Clostridium thermocellum. Mechanism of end product inhibition by ethanol. Herrero, A.A., Gomez, R.F., ... A high-mobility-group protein and its cDNAs from Drosophila melanogaster. Wagner, C.R., Hamana, K., Elgin, S.C. Mol. Cell. Biol ... an anaerobic bacterium potentially useful for the single step conversion of biomass to ethanol [14]. ... has both a high basic (24%) and high acidic (24%) amino acid content, and is a DNA-binding protein [34]. ...
  https://www.wikigenes.org/e/chem/e/24247.html
*  Cellulosic ethanol - Wikipedia
... forest biomass has significant advantages over agricultural biomass. Forest biomass also has high density which significantly ... One example is Clostridium thermocellum, which uses a complex cellulosome to break down cellulose and synthesize ethanol. ... Forest biomass has higher cellulose and lignin content and lower hemicellulose and ash content than agricultural biomass. ... Biomass and agricultural biomass. In the US, about 1.4 billion dry tons of biomass can be sustainably produced annually. About ...
  https://en.wikipedia.org/wiki/Cellulosic_ethanol