*  "Concerted effects of heterogeneous nuclear ribonucleoprotein C1/C2 to " by Rui Zhou, Juw Won Park et al.
Traditionally recognized as an RNA splicing regulator, heterogeneous nuclear ribonucleoprotein C1/C2 (hnRNPC1/C2) can also bind to double-stranded DNA and function in trans as a vitamin D response element (VDRE)-binding protein. As such, hnRNPC1/C2 may couple transcription induced by the active form of vitamin D, 1,25-dihydroxyvitamin D (1,25(OH)2D) with subsequent RNA splicing. In MG63 osteoblastic cells, increased expression of the 1,25(OH)2D target gene CYP24A1 involved immunoprecipitation of hnRNPC1/C2 with CYP24A1 chromatin and RNA. Knockdown of hnRNPC1/C2 suppressed expression of CYP24A1, but also increased expression of an exon 10-skipped CYP24A1 splice variant; in a minigene model the latter was attenuated by a functional VDRE in the CYP24A1 promoter. In genome-wide analyses, knockdown of hnRNPC1/C2 resulted in 3500 differentially expressed genes and 2232 differentially spliced genes, with ...
*  Heterogeneous nuclear ribonucleoprotein D0B is a sequence-specificDNA-binding protein | Biochemical Journal
Complement receptor 2 (CR2) is important in the regulation of the B lymphocyte response; the regulation of its expression is therefore of central importance. We recently reported that a 42 kDa heterogeneous nuclear ribonucleoprotein (hnRNP) is involved in the transcriptional regulation of the human CR2 gene [Tolnay, Lambris and Tsokos (1997) J. Immunol. 159, 5492-5501]. We cloned the cDNA encoding this protein and found it to be identical with hnRNP D0B, a sequence-specific RNA-binding protein. By using a set of mutated oligonucleotides, we demonstrated that the recombinant hnRNP D0B displays sequence specificity for double-stranded oligonucleotide defined by the CR2 promoter. We conducted electrophoretic mobility-shift assays to estimate the apparent Kd of hnRNP D0B for the double-stranded DNA motif and found it to be 59 nM. Interestingly, hnRNP D0B displayed affinities of 28 and 18 nM for the sense and anti-sense strands of the CR2 ...
*  SNRPE - Small nuclear ribonucleoprotein E - Homo sapiens (Human) - SNRPE gene & protein
Core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. As part of the U7 snRNP it is involved in histone 3'-end processing. May indirectly play a role in hair development.
*  Plus it
CDC27 is a core component of the anaphase-promoting complex/cyclosome (APC/C), a multisubunit E3 ubiquitin ligase, whose oscillatory activity is responsible for the metaphase-to-anaphase transition and mitotic exit. Here, in normal murine mammary gland epithelial cells (NMuMG), CDC27 expression is controlled posttranscriptionally through the RNA binding protein poly(rC) binding protein 1 (PCBP1)/heterogeneous nuclear ribonucleoprotein E1 (HNRNP E1). shRNA-mediated knockdown of HNRNP E1 abrogates translational silencing of the Cdc27 transcript, resulting in constitutive expression of CDC27. Dysregulated expression of CDC27 leads to premature activation of the G2-M-APC/C-CDC20 complex, resulting in the aberrant degradation of FZR1/CDH1, a cofactor of the G1 and late G2-M-APC/C and a substrate normally reserved for the SCF-βTRCP ligase. Loss of CDH1 expression and of APC/C-CDH1 activity, upon constitutive ...
*  heterogeneous nuclear ribonucleoprotein K - NB110-40898 | acris-antibodies.com
heterogeneous nuclear ribonucleoprotein K, 5 ml. The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA).
*  1x0f - Proteopedia, life in 3D
Heterogeneous nuclear ribonucleoprotein D, also known as AUF1, has two DNA/RNA-binding domains, each of which can specifically bind to single-stranded d(TTAGGG)n, the human telomeric repeat. Here, the structure of the C-terminal-binding domain (BD2) complexed with single-stranded d(TTAGGG) determined by NMR is presented. The structure has revealed that each residue of the d(TAG) segment is recognized by BD2 in a base-specific manner. The interactions deduced from the structure have been confirmed by gel retardation experiments with mutant BD2 and DNA. It is known that single-stranded DNA with the telomeric repeat tends to form a quadruplex and that the quadruplex has an inhibitory effect on telomere elongation by telomerase. This time it is revealed that BD2 unfolds the quadruplex of such DNA upon binding. Moreover, the effect of BD2 on the elongation by telomerase was examined in vitro. These results ...
*  Heterogeneous nuclear ribonucleoproteins elisa and antibody
Shop Heterogeneous nuclear ribonucleoproteins ELISA Kit, Recombinant Protein and Heterogeneous nuclear ribonucleoproteins Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
*  Anti hnRNP D Antibody (PrecisionAb™ Polyclonal Antibody) | Bio-Rad Antibodies (formerly AbD Serotec)
hnRNPD antibody for detecting human heterogeneous nuclear ribonucleoprotein D0. Validated on up to 12 cell lysates for western blotting. Try a trial size today.
*  OriGene - HNRNPD (NM 001003810) Human ORF cDNA Clone
HNRNPD - HNRNPD (Myc-DDK-tagged)-Human heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) (HNRNPD), transcript variant 4 available for purchase from OriGene - Your Gene Company.
*  HNRPD - Wikipedia
Heterogeneous nuclear ribonucleoprotein D0 (HNRNPD) also known as AU-rich element RNA-binding protein 1 (AUF1) is a protein that in humans is encoded by the HNRNPD gene. Alternative splicing of this gene results in four transcript variants. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). The interaction sites on the RNA are frequently biased towards particular sequence motifs. These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA ...
*  OriGene - HNRNPK (NM 002140) Human Protein
Recombinant protein of human heterogeneous nuclear ribonucleoprotein K (HNRNPK), transcript variant 1, 20 ug available for purchase from OriGene - Your Gene Company.
*  HNRNPA2B1 - Heterogeneous nuclear ribonucleoproteins A2/B1 - Bos taurus (Bovine) - HNRNPA2B1 gene & protein
Heterogeneous nuclear ribonucleoprotein (hnRNP) that associates with nascent pre-mRNAs, packaging them into hnRNP particles. The hnRNP particle arrangement on nascent hnRNA is non-random and sequence-dependent and serves to condense and stabilize the transcripts and minimize tangling and knotting. Packaging plays a role in various processes such as transcription, pre-mRNA processing, RNA nuclear export, subcellular location, mRNA translation and stability of mature mRNAs. Forms hnRNP particles with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleus. Involved in transport of specific mRNAs to the cytoplasm in oligodendrocytes and neurons: acts by specifically recognizing and binding the A2RE (21 nucleotide hnRNP A2 response element) or the A2RE11 ...
*  HNRNPL - Wikipedia
Heterogeneous nuclear ribonucleoprotein L is a protein that in humans is encoded by the HNRNPL gene. Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogeneous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for ...
*  Primary structures of the heterogeneous nuclear ribonucleoprotein A2, B1, and C2 proteins: a diversity of RNA binding proteins...
We have isolated cDNAs for the major heterogeneous nuclear ribonucleoprotein (hnRNP) A2, B1, and C2 proteins and determined their nucleotide and deduced amino acid sequences. The A2 and B1 cDNAs are identical except for a 36-nucleotide in-frame insert in B1. Similarly, the sequence of the C2 protein cDNA is related to that of C1 in that C2 contains an extra 39 in-frame nucleotides. Therefore, the B1 amino acid sequence is identical to A2 except for the insertion of 12 amino acids near its amino terminus, and C1 and C2 are also identical to each other except for an extra 13 amino acids near the middle of C2. All three proteins are members of a large family of RNA binding proteins that contain the consensus sequence-type RNA binding domain (CS-RBD). The A2 and B1 proteins have a modular structure similar to that of the hnRNP protein A1: they ...
*  anti-hnRNP A2B1 antibody [DP3B3] | GeneTex
hnRNP A2B1 antibody [DP3B3] (heterogeneous nuclear ribonucleoprotein A2/B1) for ELISA, ICC/IF, IHC, IP, WB. Anti-hnRNP A2B1 mAb (GTX26102) is tested in Human, Mouse, Chicken samples. 100% Ab-Assurance.
*  Multiple splicing factors are released from endogenous complexes during in vitro pre-mRNA splicing - CSHL Scientific Digital...
Pre-mRNA splicing occurs in a macromolecular complex called the spliceosome. Efforts to isolate spliceosomes from in vitro splicing reactions have been hampered by the presence of endogenous complexes that copurify with de novo spliceosomes formed on added pre-mRNA. We have found that removal of these large complexes from nuclear extracts prevents the splicing of exogenously added pre-mRNA. We therefore examined these complexes for the presence of splicing factors and proteins known or thought to be involved in RNA splicing. These fast-sedimenting structures were found to contain multiple small nuclear ribonucleoproteins (snRNPs) and a fragmented heterogeneous nuclear ribonucleoprotein complex. At least two splicing factors other than the snRNPs were also associated with these large structures. Upon incubation with ATP, ...
*  OriGene - Syncrip (NM 001047916) Human ORF cDNA Clone
Syncrip - Syncrip (Myc-DDK-tagged ORF) - Rat synaptotagmin binding, cytoplasmic RNA interacting protein (Syncrip), (10 ug) available for purchase from OriGene - Your Gene Company.
*  SYNCRIP MaxPab mouse polyclonal antibody (B01) - (H00010492-B01) - Products - Abnova
Mouse polyclonal antibody raised against a full-length human SYNCRIP protein. SYNCRIP (AAH40844, 1 a.a. ~ 410 a.a) full-length human protein. (H00010492-B01) - Products - Abnova
*  Anti-hnRNP Q Antibody [I8E4] | Immuquest
Purchase hnRNP Q Antibody [I8E4] directly from Immuquest. Validated for ICC/IF, Flow Cyt, ELISA, WB, IP in Mouse, Human, Xenopus laevis
*  Anti-hnRNP Q 抗体 (ab99152) | アブカム
ウサギ・ポリクローナル抗体 ab99152 交差種: Hu 適用: WB…hnRNP Q抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody 製品。国内在庫と品質保証制度も充実。
*  Anti-hnRNP F 抗体 (ab50982) | アブカム
ウサギ・ポリクローナル抗体 ab50982 交差種: Ms,Rat,Hu 適用: WB,ELISA,IHC-P,ICC/IF…hnRNP F抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody…
*  Anti-hnRNP L 抗体 (ab32680) | アブカム
ウサギ・ポリクローナル抗体 ab32680 交差種: Hu 適用: WB,IHC-P,ICC/IF…hnRNP L抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody 製品。国内在庫と品質保証制度も充実。
*  "A predominantly nuclear protein affecting cytoplasmic localization of " by Wei Gu, Feng Pan et al.
The localization of beta-actin mRNA to the leading lamellae of chicken fibroblasts and neurite growth cones of developing neurons requires a 54-nt localization signal (the zipcode) within the 3' untranslated region. In this study we have identified and isolated five proteins binding to the zipcode. One of these we previously identified as zipcode binding protein (ZBP)1, a 4-KH domain protein. A second is now investigated in detail: a 92-kD protein, ZBP2, that is especially abundant in extracts from embryonic brain. We show that ZBP2 is a homologue of the human hnRNP protein, KSRP, that appears to mediate pre-mRNA splicing. However, ZBP2 has a 47-amino acid (aa) sequence not present in KSRP. Various portions of ZBP2 fused to GFP indicate that the protein most likely shuttles between the nucleus and the cytoplasm, and that the 47-aa insert promotes the nuclear localization. Expression of a truncated ZBP2 ...
*  Anti-PCBP2/hnRNP E2 antibody (ab95942) | Abcam
Rabbit polyclonal PCBP2/hnRNP E2 antibody validated for WB, ICC/IF and tested in Human. Immunogen corresponding to synthetic peptide