Hepatocyte - Wikipedia
A hepatocyte is a cell of the main parenchymal tissue of the liver. Hepatocytes make up 80% of the livers mass. These cells ... In vitro model systems based on hepatocytes have been of great help to better understand the role of hepatocytes in (patho) ... The average life span of the hepatocyte is 5 months; they are able to regenerate. Hepatocyte nuclei are round with dispersed ... Smooth endoplasmic reticulum is abundant in hepatocytes, in contrast to most other cell types. Hepatocytes display an ...
hepatocytes Archives - British Liver Trust
The effects of dexamethasone on metabolic activity of hepatocytes in primary monolayer culture
The effects of dexamethasone on multiple metabolic functions of adult rat hepatocytes in monolayer culture were studied. Adult ... The effects of dexamethasone on metabolic activity of hepatocytes in primary monolayer culture In Vitro. 1980 Jul;16(7):559-70. ... The effects of dexamethasone on multiple metabolic functions of adult rat hepatocytes in monolayer culture were studied. Adult ...
increased hepatocyte apoptosis Mammalian Phenotype Term (MP:0003887)
The Mammalian Phenotype (MP) Ontology is a community effort to provide standard terms for annotating phenotypic data. You can use this browser to view terms, definitions, and term relationships in a hierarchical display. Links to summary annotated phenotype data at MGI are provided in Term Detail reports.
Advanced Cell Technology demonstrates highly efficient process for the generation of high-purity hepatocytes
has reported for the first time a robust and highly efficient process for the generation of high-purity hepatocytes (liver ... Advanced Cell Technology demonstrates highly efficient process for the generation of high-purity hepatocytes. *Download PDF ... As with other tissues, there is a scarcity of donor livers and hepatocytes, which is compounded by the low recovery and ... has reported for the first time a robust and highly efficient process for the generation of high-purity hepatocytes (liver ...
JCI - Cannabinoid receptor 1 signaling in hepatocytes and stellate cells does not contribute to NAFLD
Previous studies showed that hepatocytes are sensitive to flow sorting (35); therefore, we optimized a hepatocyte isolation ... For hepatocyte-specific deletion of Cnr1, Cnr1fl/fl mice were bred with mice expressing Cre recombinase under the control of ... Hepatocyte-specific Cnr1 deletion does not affect glucose tolerance or insulin sensitivity in mice fed chow or HSD. Mice used ... Hepatocyte Cnr1 deletion did not alter plasma glucose and insulin levels (Figure 3, E and F). These studies demonstrated that ...
JCI - Cannabinoid receptor 1 signaling in hepatocytes and stellate cells does not contribute to NAFLD
Deletion of CB-1 in hepatocytes did not alter the development of NAFLD in mice fed a high-sucrose diet (HSD) or a high-fat diet ... Here, we produced mice that lacked CB-1 in hepatocytes or stellate cells to determine if CB-1 signaling contributes to the ... Combined, these studies do not support a direct role for hepatocyte or stellate cell CB-1 signaling in the development of NAFLD ... Cannabinoid receptor 1 signaling in hepatocytes and stellate cells does not contribute to NAFLD. ...
Partial pancreatic transdifferentiation of primary human hepatocytes in the livers of a humanised mouse model
These data show that human hepatocytes can be induced to undergo partial pancreatic transdifferentiation in vivo, indicating ... Partial pancreatic transdifferentiation of primary human hepatocytes in the livers of a humanised mouse model J Gene Med. 2018 ... Methods: Engraftment of human hepatocytes was confirmed by measuring human albumin levels. Following delivery of the empty ... Conclusions: These data show that human hepatocytes can be induced to undergo partial pancreatic transdifferentiation in vivo, ...
Liver, Hepatocyte - Glycogen Accumulation and Depletion - Nonneoplastic Lesion Atlas
... the degree of glycogen accumulation within hepatocytes is typically highest in the early morning hours and wanes throughout the ... It initially disappears in the centrilobular hepatocytes (Figure 2. ), with progression to the periportal hepatocytes during ... Liver, Hepatocyte - Glycogen Accumulation and Depletion Narrative. Comment:. Because rodents typically feed at night, the ... There is a notable depletion of glycogen within hepatocytes. Excess glycogen accumulation beyond that present in Figure 1. and ...
BioIVT Webinar Will Discuss How Primary Hepatocytes Are Enabling Antimalarial Drug Discovery - MassBio
They include large lots of highly viable, consistent cryopreserved human hepatocytes, and cynomolgus and rhesus hepatocytes, ... As these hepatocytes can mimic liver properties, they allow his team to evaluate the metabolism, drug-drug interaction, and ... Maher will discuss the drug discovery platform, review complex and multiplex in vitro systems for primary hepatocyte culture, ... Researchers at the University of Georgia used hepatocytes to develop the first medium-throughput, 384-well plate-based liver- ...
Engineering the 3D Niche to enHANCE functionality of hepatocytes derived from human iPSCs | ENHANCE | Project | Fact sheet |...
Induced pluripotent stem cell derived hepatocytes (i-Heps) afford us the exciting possibility of redressing the growing ... Induced pluripotent stem cell derived hepatocytes (i-Heps) afford us the exciting possibility of redressing the growing ... Engineering the 3D Niche to enHANCE functionality of hepatocytes derived from human iPSCs. ... freshly isolated primary hepatocytes. Emerging evidence suggests the three dimensional environment surrounding a cell (the ...
Quantitative characterisation of ageing hepatocytes | Université de Liège
the volume of hepatocyte nuclei increases from 410 µm3 at birth to 840 µm3 at the 12th month and decreases thereafter to 470 µm ... the volume of hepatocytes increases from 5800 µm3 to about 10000 µm3 in 6 months and then decreases to 7300 µm3 (24th-27th ... the number of hepatocytes increases from 41.4 x 106 at birth to 1150 x 106 (6th month). ... Hepatocytes of rats of various ages were investigated morphometrically and qualitatively.. During the postnatal development and ...
SCOPe 2.08: Protein: Hepatocyte nuclear factor 4-alpha
Timeline for Protein Hepatocyte nuclear factor 4-alpha from a.123.1.1: Nuclear receptor ligand-binding domain: *Protein ... Lineage for Protein: Hepatocyte nuclear factor 4-alpha. *Root: SCOPe 2.08 *. Class a: All alpha proteins [46456] (290 folds). ... Protein Hepatocyte nuclear factor 4-alpha from a.123.1.1: Nuclear receptor ligand-binding domain appears in SCOPe 2.07. ... More info for Protein Hepatocyte nuclear factor 4-alpha from a.123.1.1: Nuclear receptor ligand-binding domain. ...
Signal transduction pathways in hepatocyte cell death. New targets for therapy | News articles | University of Groningen
Three models of hepatocyte cell death were studied: glycochenodeoxycholic acid (GCDCA)-induced cell death as a model for bile ... Hepatocyte cell death can occur via either apoptosis or necrosis (or intermediate forms). Knowledge about the cellular ... The aim of this thesis is to elucidate the mechanisms of cell death in hepatocytes, in order to develop strategies to protect ... This thesis describes that hepatocyte death and survival are regulated at the cross-roads of intracellular organelles (e.g., ...
Inflammatory Cytokine TNFalpha Promotes the Long-Term Expansion of Primary Hepatocytes in 3D Culture. - CIRM
Single-cell RNA sequencing reveals broad expression of hepatocyte markers. Strikingly, in vitro-expanded hepatocytes engrafted ... Single-cell RNA sequencing reveals broad expression of hepatocyte markers. Strikingly, in vitro-expanded hepatocytes engrafted ... Inflammatory Cytokine TNFalpha Promotes the Long-Term Expansion of Primary Hepatocytes in 3D Culture.. Return to Grants ... Currently, liver organoids can be generated readily in vitro from bile-duct epithelial cells, but not hepatocytes. Here, we ...
Receptor-Type Protein Tyrosine Phosphatase ϵ (PTPϵM) is a Negative Regulator of Insulin Signaling in Primary Hepatocytes and...
In this study, we showed that receptor-type PTPϵ (PTPϵM) dephosphorylated IR in rat primary hepatocytes and tyrosines 972, 1158 ... Receptor-Type Protein Tyrosine Phosphatase ϵ (PTPϵM) is a Negative Regulator of Insulin Signaling in Primary Hepatocytes and ... is a Negative Regulator of Insulin Signaling in Primary Hepatocytes and Liver," Zoological Science 22(2), 169-175, (1 February ... expression in primary hepatocytes. Furthermore, adenovirally introduced PTPϵM also exhibited inhibitory activity against ...
Abstract: Stiffness Mediated Regulation of Hepatocyte Functions (2015 Annual Meeting)
We further demonstrated that hepatocyte cultured on soft substrate showed better maintenance of hepatocyte-specific functions ... The intricate LME-hepatocytes signaling pathways through which healthy and diseased liver cells communicate within the LME will ... In our study, we investigated the effect of substrate stiffness on the functions of cultured primary hepatocytes utilizing a ... Together, these results indicate that the substrate stiffness plays a significant role in modulating hepatocyte behavior. ...
Metabolism and Toxicity of Thioacetamide and Thioacetamide SOxide in Rat Hepatocytes
On the other hand, TASO was highly toxic to isolated hepatocytes as indicated by LDH release, cellular morphology and vital ... The improved understanding of the metabolism and covalent binding of TA and TASO facilitates the use of hepatocytes to prepare ... Significantly, we found that hepatocytes produce TA from TASO relatively efficiently by back-reduction. The covalent binding of ... we treated isolated hepatocytes directly with TA. Surprisingly, TA was not toxic at concentrations up to 50 mM for 40 hr. ...
Frontiers | In vitro Modulation of the LPS-Induced Proinflammatory Profile of Hepatocytes and Macrophages- Approaches for...
In this study, macrophages (J774) and hepatocytes (HepG2) were stimulated with lipopolysaccharide (LPS) from E. coli 0111: B4 ... In this study, macrophages (J774) and hepatocytes (HepG2) were stimulated with lipopolysaccharide (LPS) from E.coli 0111: B4 ... These inflammatory mediators further stimulate hepatocytes and hepatic satellite cells to induce hepatocyte steatosis and ... Effect of DHA and Vitamin D3 on LPS-Induced mRNA Expression of Srepb-1c and PPARα by Macrophages and Hepatocytes. LPS exposure ...
Dendritic Cells and Hepatocytes Use Distinct Pathways to Process Protective Antigen from Plasmodium in vivo | PLOS Pathogens
BioreclamationIVT and Qualyst partner to market Transporter-Certified hepatocytes
... the intracellular concentrations in Transporter Certified hepatocytes reflect physiologic conditions because the hepatocytes ... "These hepatocytes perform at the highest level because we uniquely characterise and certify their transporter and bile acid ... Specifically, Transporter Certified hepatocytes can be used for transporter studies, quantitation of basolateral versus biliary ... Qualysts patented B-CLEAR Technology is used to evaluate hepatocyte lots and qualify them as Transporter Certified. B-CLEAR ...
Induction of CYP2C Genes in Human Hepatocytes in Primary Culture | Drug Metabolism & Disposition
Induction of CYP2C Genes in Human Hepatocytes in Primary Culture. Sabine Gerbal-Chaloin, Jean-Marc Pascussi, Lydiane Pichard- ... Induction of CYP2C Genes in Human Hepatocytes in Primary Culture Message Subject (Your Name) has forwarded a page to you from ... Induction of CYP2C Genes in Human Hepatocytes in Primary Culture. Sabine Gerbal-Chaloin, Jean-Marc Pascussi, Lydiane Pichard- ... Induction of CYP2C Genes in Human Hepatocytes in Primary Culture. Sabine Gerbal-Chaloin, Jean-Marc Pascussi, Lydiane Pichard- ...
RCSB PDB - 4AVX: Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate (Hgs-Hrs) bound to an IP2 compound at 1.68 A...
Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate (Hgs-Hrs) bound to an IP2 compound at 1.68 A Resolution ... Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate (Hgs-Hrs) bound to an IP2 compound at 1.68 A Resolution. *PDB DOI ... Crystal Structure of the Tandem Vhs and Fyve Domains of Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate (Hgs-Hrs) ...
Plus it
2009) Hepatocyte growth factor protects auditory hair cells from aminoglycosides. Laryngoscope 119:2027-2031, doi:10.1002/lary. ... 1995) Scatter factor/hepatocyte growth factor is essential for liver development. Nature 373:699-702, doi:10.1038/373699a0, ... 2000) Hepatocyte growth factor-stimulated invasiveness of monocytes. Blood 95:3964-3969, pmid:10845935. ... 1999) Hepatocyte growth factor/scatter factor-MET signaling in neural crest-derived melanocyte development. Pigment Cell Res 12 ...
Biological roles of hepatocyte growth factor‑Met signaling from genetically modified animals (Review)
Hepatocyte growth factor (HGF). HGF was cloned as a growth factor for hepatocytes (1,2), is identical to scatter factor (SF) ... Shiota G, Wang TC, Nakamura T and Schmidt EV: Hepatocyte growth factor in transgenic mice: Effects on hepatocyte growth, liver ... Decrease of hepatocyte migration and proliferation.. (33-35). Defects in redox regulation. Failure of hepatic stem cell ... Hepatocytes (oval cells). Alb, Mx1, Alfp. Increased liver damage and fibrosis. Impaired liver regeneration.. (33-39). ...
EVALUATION OF TIME-DEPENDENT CYTOCHROME P450 INHIBITION USING CULTURED HUMAN HEPATOCYTES | Drug Metabolism & Disposition
Preparation of Human Hepatocytes. Human hepatocytes were prepared from an isolated lobe of human liver (obtained from local ... Culturing of Human Hepatocytes. Freshly isolated hepatocytes were diluted to a cell concentration of approximately 8 × 106/ml ... Time-Dependent Inhibition of P450 Activity in Cultured Human Hepatocytes. The limited number of hepatocytes from each ... CYP3A4, 2C19, and 2D6 activity in cultured human hepatocytes after incubation with fluoxetine. Using cultured human hepatocytes ...
Hepatic Tissue Environment in NEMO-Deficient Mice Critically Regulates Positive Selection of Donor Cells after Hepatocyte...
The deletion of the I-kappa B kinase-regulatory subunit IKKγ/NEMO in hepatocytes prevents nuclear factor (NF)-kB activation and ... Methods Pre-conditioned NEMOΔhepa mice were transplanted with donor-hepatocytes from wildtype (WT) and mice deficient for the ... Background Hepatocyte transplantation (HT) is a promising alternative treatment strategy for end-stage liver diseases compared ... pro-apoptotic mediator Caspase-8 (Casp8Δhepa). Results Transplantation of isolated WT-hepatocytes into pre-conditioned NEMO ...
Mechanisms of Acetaminophen-Induced Hepatotoxicity: Role of Oxidative Stress and Mitochondrial Permeability Transition in...
Freshly isolated mouse hepatocytes were used to determine the role of mitochondrial permeability transition (MPT) in ... Incubation of APAP (1 mM) with hepatocytes resulted in cell death as indicated by increased alanine aminotransferase in the ... To separate metabolic events from later events in toxicity, hepatocytes were preincubated with APAP for 2 h followed by ... Role of Oxidative Stress and Mitochondrial Permeability Transition in Freshly Isolated Mouse Hepatocytes. Angela B. Reid, ...
Hepatocyte Nuclear Factors | Profiles RNS
"Hepatocyte Nuclear Factors" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH ( ... Hepatocyte nuclear factors are a family of evolutionarily conserved transcription factors that are preferentially expressed in ... This graph shows the total number of publications written about "Hepatocyte Nuclear Factors" by people in this website by year ... Below are the most recent publications written about "Hepatocyte Nuclear Factors" by people in Profiles. ...
RPPA Reveals Altered Phosphorylation Profile in Plasmodium-infected Hepatocytes - Grace Bio-Labs
The parasite infects the host by traveling to the liver where it intracellularly develops and affects hepatocyte cells, ... Different hosts, and even different hepatocyte cells within a single host exhibit different capacities to support or limit ... The RPPA assay was constructed using ONCYTE nitrocellulose slides arrayed with protein lysates of the different hepatocyte ... Kaushansky, et al, investigated differential susceptibility to infection of hepatocytes populations of BALB/cJ and BALB/cByJ ...