Molecular and functional characterization of drug-metabolizing enzymes and transporter expression in the novel spontaneously...
In toxicological research, immortalized human hepatocytes provide a useful alternative to primary hepatocytes because ... The purpose of this study was to characterize a novel spontaneously immortalized human hepatocyte cell line, HC-04, with ... HC-04 cells retained hepatocyte-specific function including albumin production and ornithine transcarbamoylase and glucose-6- ... at a rate similar to primary human hepatocytes. Furthermore, the major human sulfotransferase and UDP-glucuronosyltransferase ...http://scholarbank.nus.edu.sg/handle/10635/28873
AID 353102 - Therapeutic index, ratio of CC50 for human hepatocytes to IC50 for HCV NS3-4A protease - PubChem
... ratio of CC50 for human hepatocytes to IC50 for HCV NS3-4A protease. ...https://pubchem.ncbi.nlm.nih.gov/bioassay/353102
Hepatocyte transplantation in bile salt export pump-deficient mice: selective growth advantage of donor hepatocytes under bile...
Hepatocyte transplantation in bile salt export pump-deficient mice: selective growth advantage of donor hepatocytes under bile ... Hepatocyte transplantation in bile salt export pump-deficient mice: selective growth advantage of donor hepatocytes under bile ... Modulation of rat hepatocyte proliferation by bile salts: in vitro and in vivo studies. Hepatology. 1996; 23: 1159-66.. *PubMed ... Hepatocytes corrected by gene therapy are selected in vivo in a murine model of hereditary tyrosinaemia type I. Nat Genet. 1996 ...http://onlinelibrary.wiley.com/doi/10.1111/j.1582-4934.2012.01586.x/references?globalMessage=0
Hepatocyte Incubations.. Human, rat, or dog cryopreserved hepatocytes were thawed and prepared in Williams' E buffer (pH = 7.4 ... All the metabolites that were detected in human hepatocytes were also found either in rat or dog hepatocytes. M1, M2, and M4 ... All the metabolites detected in human hepatocytes were also formed either in rat or dog hepatocytes. The structures of ... human hepatocytes showed that the metabolites detected in human hepatocytes were also found in either rat or dog hepatocytes. ...http://dmd.aspetjournals.org/content/39/10/1883
In Vitro Metabolism of Thyroxine by Rat and Human Hepatocytes | Science Inventory | US EPA
Rat hepatocytes had as much as 13-times higher concentrations of metabolites in the media compared to human hepatocytes. ... Rat hepatocytes had as much as 13-times higher concentrations of metabolites in the media compared to human hepatocytes. ... Rat hepatocytes, human hepatocytes, Phase II metabolic enzymes, ISTD-STICS-12-081, thyroid hormone, thyroxine, ORD-003013, ... The hepatocytes were fed daily with William's E media containing 0.1% DMSO. Up to five days after plating, cell culture media ...https://cfpub.epa.gov/si/si_public_record_report.cfm?dirEntryId=275394
Human extracellular matrix simultaneously enhances proliferation and function in 3-D spheroids of human hepatocyte cell lines ...
Human extracellular matrix simultaneously enhances proliferation and function in 3-D spheroids of human hepatocyte cell lines. ... Human extracellular matrix simultaneously enhances proliferation and function in 3-D spheroids of human hepatocyte cell lines ... Human extracellular matrix simultaneously enhances proliferation and function in 3-D spheroids of human hepatocyte cell lines. ...http://discovery.ucl.ac.uk/1325507/
Morphogenetic events in mixed cultures of rat hepatocytes and nonparenchymal cells maintained in biological matrices in the...
Hepatocytes were grown in chemically defined hepatocyte growth medium (HGM) containing hepatocyte growth factor (HGF) and ... Many single, long plates of hepatocytes were seen, suggesting progressive linear assembly guided by hepatocyte specific ... Desmin-positive cells surrounded hepatocytes. Collagen types I and III were deposited in a diffuse manner whereas collagen type ... Morphogenetic events in mixed cultures of rat hepatocytes and nonparenchymal cells maintained in biological matrices in the ...http://onlinelibrary.wiley.com/doi/10.1002/hep.510290149/abstract
The mouse with a human liver: a new model for the treatment of liver disease - Salk Institute for Biological Studies
... but this is not possible with liver cells or hepatocytes. "Human hepatocytes are almost impossible to work with as they don't ... Mice whose own hepatocytes have been replaced with human liver cells provide a solution to all these hurdles. ... Mice whose own liver cells have been replaced with human hepatocytes (shown in green) can be successfully infected with ... Explains Verma, "This robust model system opens the door to utilize human hepatocytes for purposes that were previously ...http://www.salk.edu/news-release/the-mouse-with-a-human-liver-a-new-model-for-the-treatment-of-liver-disease/
Since there is still a debate on whether GLP-1 receptor (GLP-1R) is expressed in hepatocytes (12, 13, 36, 76), it has not been ... In mouse hepatocytes, GLP-128-36 treatment activated protein kinase A (PKA) and the Wnt signaling pathway effector β-catenin (β ... A few studies have supported the notion that GLP-1R is also expressed in hepatocytes (26, 36, 77, 84), whereas a number of ... GLP-1 (9-36) amide metabolite suppression of glucose production in isolated mouse hepatocytes. Horm Metab Res 42: 657-662, 2010 ...http://ajpendo.physiology.org/content/311/3/E620
Exploring interactions between rat hepatocytes and nonparenchymal cells using gene expression profiling - Khetani - 2004 -...
Drug metabolizing enzymes in rat hepatocytes co-cultured with cell lines. In Vitro Cell Dev Biol 1990; 26: 1057-1062.. * ... Cooperation of Ito cells and hepatocytes in the deposition of an extracellular matrix in vitro. Am J Pathol 1993; 143: 538-544. ... Hepatocyte migration during liver development requires Prox1. Nat Genet 2000; 25: 254-255.. *CrossRef, ... Long-term in vitro function of adult hepatocytes in a collagen sandwich configuration. Biotechnol Prog 1991; 7: 237-245.. ...http://onlinelibrary.wiley.com/doi/10.1002/hep.20351/references?globalMessage=0
Hepatotoxicity Cytotoxicity Assays | China-Mainland | Sigma-Aldrich
Apart from human hepatocytes, HepaRG cells are the most metabolically active liver cell line described to date and have the ... Primary human hepatocytes are the gold standard for these types of studies, but limited availability, high cost, donor-to-donor ...https://www.sigmaaldrich.com/china-mainland/zh/life-science/adme-tox-assays/hepatotoxicity-cytotoxicity-assays.html
class notes and powerpoints - • Utilizes both glycolytic and oxidative pathways • Liver cell hepatocyte functions •...
Utilizes both glycolytic and oxidative pathways • Liver cell ( hepatocyte) functions: • Carbohydrate, lipid and amino acid ... Unformatted text preview: • Utilizes both glycolytic and oxidative pathways • Liver cell ( hepatocyte) functions: • ...https://www.coursehero.com/file/1679478/class-notes-and-powerpoints/
iPS Cell to Hepatocyte Differentiation System
iPS cell-derived hepatocytes resemble primary hepatocytes and express mature hepatocyte markers. To evaluate the robustness and ... iPS cell-derived hepatocytes reflect maturing hepatocyte mRNA expression profiles. mRNA expression of several hepatocyte ... fetal-like hepatocyte and mature hepatocyte. This process takes another two weeks. At the end of this process, hepatocyte ... The iPS-derived hepatocytes express markers typical of mature hepatocytes such as HNFα, albumin, PXR, and Phase I metabolizing ...http://www.clontech.com/US/Products/Stem_Cell_Research/Resources/Technical_Notes/iPSC-derived-hepatocytes?sitex=10020:22372:US&PEBCL1=q8DIRG9bPvMbTPhbC49c9ONqG5&PEBCL1_pses=ZG7AFC907621D7D9B47296560AA304D443226DB8FD4546DE161C4122758BE6DAF83AA0A21DAD20AEFF0C559D6E161D0F78B40BF35311019719
Isolation and culture of adult human liver progenitor cells: i...
... in vitro differentiation to hepatocyte-like cells.: Highly differentiated normal human hepatocytes ... Isolation and culture of adult human liver progenitor cells: in vitro differentiation to hepatocyte-like cells.. Authors * ... Under appropriate conditions, these cells differentiate in vitro in hepatocyte-like cells and therefore appear to behave as ... Highly differentiated normal human hepatocytes represent the gold standard cellular model for basic and applied research in ...https://www.mysciencework.com/publication/show/isolation-culture-adult-human-liver-progenitor-cells-vitro-differentiation-hepatocyte-like-cells-179b7954
iPS Cell to Hepatocyte Differentiation System
... functional hepatocytes from human induced pluripotent stem cells. ... The Cellartis iPS Cell to Hepatocyte Differentiation System ... Hepatocytes obtained with the Cellartis iPS Cell to Hepatocyte Differentiation System express major hepatic markers and show ... Hepatocytes derived from human induced pluripotent stem (hiPS) cells using the Cellartis iPS Cell to Hepatocyte Differentiation ... Cellartis Hepatocyte Differentiation Kit (Y30050). References. *Asplund, A., et al. (2015) Stem Cell Rev. and Rep. Advance ...http://www.clontech.com/US/Products/Stem_Cell_Research/Pluripotency_and_Differentiation/iPS_Cell_to_Hepatocyte_Differentiation_System?sitex=10020:22372:US&PEBCL1=6du0l11nLZ1hFC5C84u1YrXpqn&PEBCL1_pses=ZG82761168F143A8A140B60F080A7AE7551D2F5C575A3ABD2EBEA2D6F7EE892B24FE15697F39EBB3405C5C15F3CB91E2EF3D1E2D13697095F3
Primary Hepatocytes | Human and Animal Hepatocytes | Corning
Primary Animal Hepatocytes Primary Animal Hepatocytes Learn More *Characterized for platability and many metabolic enzyme ... High Quality Primary Human Hepatocytes High Quality Primary Human Hepatocytes Learn More *Pre-screened and characterized for ... Cyropreserved hepatocytes from common pre-clinical animal species are also available. All primary hepatocyte products are pre- ... Need to Outsource Your Hepatocyte Studies? Need to Outsource Your Hepatocyte Studies? Utilize Corning Expertise to get the ...https://www.corning.com/in/en/products/life-sciences/products/adme-tox-research/primary-hepatocytes.html
Hepatocytes | Gastrointestinal and Liver Physiology
Role of apoptotic hepatocytes in HCV dissemination: regulation by acetaldehyde Murali Ganesan, Sathish Kumar Natarajan, Jinjin ... Interleukin-1 inhibits the induction of insulin-like growth factor-I by growth hormone in CWSV-1 hepatocytes Margaret L. ... Targeted inactivation of copper transporter Atp7b in hepatocytes causes liver steatosis and obesity in mice Abigael Muchenditsi ... Follistatin attenuates early liver fibrosis: effects on hepatic stellate cell activation and hepatocyte apoptosis Shane Patella ...http://ajpgi.physiology.org/keyword/hepatocytes
Metabolism of rT3 by Isolated Rat Hepatocytes | SpringerLink
Rooda S.J.E., van Loon M.A.C., Visser T.J. (1986) Metabolism of rT3 by Isolated Rat Hepatocytes. In: Medeiros-Neto G., Gaitan E ...https://link.springer.com/chapter/10.1007/978-1-4684-5260-0_76
Hepatocyte Growth Factor | SpringerLink
... was originally detected in the circulation of hepatectomized animals on the basis of its ability to stimulate hepatocyte ... Hepatocyte growth factor inhibits growth of hepatocellular carcinoma cells. Proc Natl Acad Sci USA 1992;89:373-7.PubMedCrossRef ... Hepatocyte growth factor enhances B-cell activity. Life Sci 1993;53:PL89-93.PubMedCrossRefGoogle Scholar ... Scatter factor/hepatocyte growth factor is essential for liver development. Nature 1995;373:699-702.PubMedCrossRefGoogle ...https://link.springer.com/chapter/10.1007/978-1-4612-1876-0_18
Stem Cell-Derived Hepatocytes
Enhanced hiPS-HEP cells are a highly homogenous population of hepatocytes derived from human induced pluripotent stem (iPS) ... Hepatocyte long-term maintenance medium. Additional Information. Please see the product's Certificate of Analysis for ... Cellartis enhanced hiPS-HEP iPSC-derived hepatocytes. Cellartis enhanced hiPS-HEP v2 kits provide an inexhaustible supply of ... Cells express key hepatocyte proteins, including α1AT, Alb, CK18, HNF4α, and E-cadherin ...http://www.clontech.com/US/Products/Stem_Cell_Research/Cells/Human_Stem_Cell_Derived_Hepatocytes?sitex=10020:22372:US&PEBCL1=6du0l11nLZ1hFC5C84u1YrXpqn&PEBCL1_pses=ZG82761168F143A8A140B60F080A7AE7551D2F5C575A3ABD2EBEA2D6F7EE892B24FE15697F39EBB3405C5C15F3CB91E2EF3D1E2D13697095F3
The Preparation and Culturing of Rat Hepatocytes | SpringerLink
... cultured hepatocytes would seem at first sight to be an ideal model system for the study of hepatocarcinogenesis and the ... Isolated, cultured hepatocytes would seem at first sight to be an ideal model system for the study of hepatocarcinogenesis and ... Paine A (1990).The maintenance of cytochrome P$%) in rat hepatocyte cultures: Some applications of liver cell cultures to the ... Guillouzo A., Morel F.,Fardel O., and Meunier B. (1993) Use of human hepatocyte cultures for drug metabolism studies.Toxicology ...https://link.springer.com/chapter/10.1007/978-3-642-79215-1_23
Intracytoplasmic Crystalline Inclusions in the Hepatocytes of an Antelope
... Sanjeev Gumber,1 Eric Lombardini,2 Bruce Williams,2 ... This case report describes intracytoplasmic crystalline inclusions in the hepatocytes of a 13-year-old female Thomson's gazelle ... Von Kossa staining showed the presence of calcium within cytoplasm, mainly in the inclusions, of hepatocytes. Transmission ... Histologically, multifocal to coalescing areas of many hepatocytes contained large cytoplasmic vacuoles filled with pale ...https://www.hindawi.com/journals/vmi/2010/373698/abs/
Human Stem Cell-derived Hepatocytes Regenerate Liver Function: Study
Functional hepatocytes from human stem cells were found to increase the survival of mice with acute liver injury, say ... Human Stem Cell-derived Hepatocytes Regenerate Liver Function: Study. by Sheela Philomena on July 28, 2013 at 10:18 AM Genetics ... Functional hepatocytes from human stem cells were found to increase the survival of mice with acute liver injury, say ... The researchers "developed a system for mass manufacture of stem cell derived hepatocytes in numbers that would be useful for ...https://www.medindia.net/news/human-stem-cell-derived-hepatocytes-regenerate-liver-function-study-122716-1.htm
Ritonavir-induced hepatotoxicity and ultrastructural changes of hepatocytes. | Sigma-Aldrich
Ritonavir-induced hepatotoxicity and ultrastructural changes of hepatocytes.. [Chang-Chun Kuang, Ying Wang, Peng-Chao Hu, Fang- ... In conclusion, the mechanism of ritonavir's hepatotoxicity is that it induces apoptosis of hepatocytes via the caspase-cascade ... To investigate the effect of ritonavir on hepatocyte proliferation, we detected the change of cleaved caspase-3 expression ... Furthermore, the morphological and ultrastructural changes of hepatocytes derived from RTV-treated mice have been observed. The ...https://www.sigmaaldrich.com/catalog/papers/25079492
Metabolism of Acrylonitrile by Isolated Rat Hepatocytes | Cancer Research
Metabolism of Acrylonitrile by Isolated Rat Hepatocytes. Lee E. Geiger, Lora L. Hogy and F. Peter Guengerich ... Metabolism of Acrylonitrile by Isolated Rat Hepatocytes Message Subject (Your Name) has forwarded a page to you from Cancer ... 2-Cyanoethylene oxide accumulated in the hepatocyte incubations but did not appear to contribute extensively to alkylation of ... Phenotypic Characterization of Metaplastic Intestinal Glands and Ductular Hepatocytes in Cholangiofibrotic Lesions Rapidly ...http://cancerres.aacrjournals.org/content/43/7/3080