Heparin and Heparan Sulfate Binding Chimeric Vectors<...
Heparin and Heparan Sulfate Binding Chimeric Vectors. Richard Jude Samulski (Inventor), Zhijian Wu (Inventor), Aravind Asokan ( ... Heparin and Heparan Sulfate Binding Chimeric Vectors. / Samulski, Richard Jude (Inventor); Wu, Zhijian (Inventor); Asokan, ... Samulski, RJ, Wu, Z & Asokan, A 1800, Heparin and Heparan Sulfate Binding Chimeric Vectors, Patent No. 7867484, IPC No. C12N 15 ... title = "Heparin and Heparan Sulfate Binding Chimeric Vectors",. abstract = "The present invention is based on the finding that ...https://uncch.pure.elsevier.com/en/publications/heparin-and-heparan-sulfate-binding-chimeric-vectors
List of MeSH codes (D09) - Wikipedia
... heparitin sulfate MeSH D09.698.373.425.500 --- heparan sulfate proteoglycan MeSH D09.698.373.475 --- hyaluronic acid MeSH ... dermatan sulfate MeSH D09.698.373.400 --- heparin MeSH D09.698.373.400.300 --- heparin, low-molecular-weight MeSH D09.698. ... heparan sulfate proteoglycan MeSH D09.698.735.800 --- platelet factor 4 MeSH D09.698.735.830 --- proteochondroitin sulfates ... dextran sulfate MeSH D09.698.365.272.400 --- iron-dextran complex MeSH D09.698.365.388 --- glycogen MeSH D09.698.365.388.518 ...https://en.wikipedia.org/wiki/List_of_MeSH_codes_(D09)
heparan sulfate)-glucosamine N-sulfotransferase - Wikipedia
... heparan sulfate]-glucosamine N-sulfotransferase. Other names in common use include heparin N-sulfotransferase, 3'- ... heparitin sulfotransferase, and 3'-phosphoadenylyl-sulfate:heparitin N-sulfotransferase. Suzuki S, Trenn RH, Strominger JL ( ... N-heparan sulfate sulfotransferase, heparan sulfate N-deacetylase/N-sulfotransferase, heparan sulfate 2-N-sulfotransferase, ... heparan sulfate]-N-sulfoglucosamine Thus, the two substrates of this enzyme are 3'-phosphoadenylyl sulfate and heparan sulfate- ...https://en.wikipedia.org/wiki/(heparan_sulfate)-glucosamine_N-sulfotransferase
Glycosaminoglycans in human retinoblastoma cells: Heparan sulfate, a modulator of the pigment epithelium-derived factor...
Hovingh P, Linker A: The enzymatic degradation of heparin and heparitin sulfate. 3. Purification of a heparitinase and a ... PEDFglycosaminoglycansheparan sulfateheparinretinoblastoma cellsreceptor binding. Background. Glycosaminoglycans (GAGs) are ... These data indicate that retinoblastoma cells secrete heparin/heparan sulfate with binding affinity for PEDF, which may be ... PEDF also has binding affinity for GAGs, such as, heparin, heparin- and chondroitin-sulfates, but this affinity is ~1000-fold ...https://bmcbiochem.biomedcentral.com/articles/10.1186/1471-2091-4-1
Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources - Board of Regents, The University of Texas...
... wherein the sulfated polysaccharide comprises heparin or heparitin sulfate. 28. The method of claim 25, wherein the sulfated ... For example, heparitin sulfate (heparan sulfate), would also suffice in the polyanion-containing protein-dissociating ... such as those containing sulfated polysaccharides (i.e., heparin or heparitin sulfate), for the isolation of nucleic acids (RNA ... wherein the sulfated polysaccharide comprises heparitin sulfate. 30. The method of claim 28, wherein the sulfated ...http://www.freepatentsonline.com/5128247.html
6-0-SULFATED POLYSACCHARIDES AND METHODS OF PREPARATION THEREOF - Patent application
... heparan sulfate(s), heparin(s), heparin sulfate(s), heparitin(s), heparitin sulfate(s), heparanoid(s), heparosan(s). The ... 0037] Heparan sulfate. As used herein, the term "heparan sulfate" or the abbreviation "HS" means a polysaccharide made up of ... 0034] Anticoagulant heparan sulfate (HSact). As used herein the term "anticoagulant heparan sulfate" or the abbreviation "HSact ... HS derived from wild-type CHO cells, N,O-desulfated, re-N-sulfated heparin (CDSNS-heparin), and 6-O-desulfated heparin was ...http://www.patentsencyclopedia.com/app/20120202706
EC 2.8.1 and EC 2.8.2
... phosphoadenylyl-sulfate:heparitin N-sulfotransferase. Systematic name: 3'-phosphoadenylyl-sulfate:[heparan sulfate]-glucosamine ... heparan sulfate: for definition click here. Other name(s): heparin-glucosamine 3-O-sulfotransferase; 3'-phosphoadenylyl-sulfate ... heparan sulfate N-deacetylase/N-sulfotransferase; heparan sulfate 2-N-sulfotransferase; heparan sulfate N-sulfotransferase; ... heparan sulfate]-glucosamine 3-sulfate. Glossary: 3'-phosphoadenylyl sulfate = PAPS. heparan sulfate: for definition click here ...http://www.sbcs.qmul.ac.uk/iubmb/enzyme/EC2/0801p.html
Investigating the mechanism of the assembly of FGF1-binding heparan sulfate motifs. - PubMed - NCBI
Enzymatic 2-O- sulfation of CDSNS-heparin polysaccharide.. Investigating the mechanism of the assembly of FGF1-binding heparan ... Heparitin Sulfate. Grant support. *R01 GM075168-05/GM/NIGMS NIH HHS/United States ... Investigating the mechanism of the assembly of FGF1-binding heparan sulfate motifs.. Nguyen TK1, Raman K, Tran VM, Kuberan B. ... Heparan sulfate (HS) chains play crucial biological roles by binding to various signaling molecules including fibroblast growth ...https://www.ncbi.nlm.nih.gov/pubmed/21803043
US5032679A - Heparin fragments as inhibitors of smooth muscle cell proliferation - Google Patents
... composition predominantly of tetrasacchardie units derived from heparin/heparan sulfate has enchanced activity in preventing ... Structural studies on heparitin sulfate of normal and Hurler tissues. Pusztai et al. 1961. Studies in immunochemistry. 18. The ... and the aldouronic acid is mostly L-iduronic in heparin and mostly D-glucuronic acid in heparan sulfate. Heparan sulfate is ... A preferred source of heparin/heparan sulfate starting material is porcine intestinal mucosa, and preparations labeled "heparin ...https://patents.google.com/patent/US5032679?oq=flatulence
Rosetting FCR3S1.2-PfEMP1 binds to heparan sulfate. All | Open-i
Rosetting FCR3S1.2-PfEMP1 binds to heparan sulfate. All the gels are 10% SDS-PAGE stained with Coomassie. A shows the expressed ... The adhesion could be competed out in a dose dependent manner with heparin or heparan sulfate but not with chondroitin sulfate ... Therefore, heparan sulfate or a heparan sulfate-like GAG seems to be the binding target for this PfEMP1. ... Therefore, heparan sulfate or a heparan sulfate-like GAG seems to be the binding target for this PfEMP1. ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC2199182_JEM.971645f3e&req=4
Identification of rosetting PfEMP1. (A) Rosetting, sing | Open-i
... to a heparin-Sepharose matrix.The adhesive interactions could be inhibited with heparan sulfate or enzymes that remove heparan ... PfEMP1 is suggested to be the rosetting ligand and heparan sulfate, or a heparan sulfate-like molecule, the receptor both for ... PfEMP1 is suggested to be the rosetting ligand and heparan sulfate, or a heparan sulfate-like molecule, the receptor both for ... The adhesive interactions could be inhibited with heparan sulfate or enzymes that remove heparan sulfate from the cell surface ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC2199182_JEM.971645f1a&req=4
A scissor blade-like closing mechanism implicated in transmembrane signaling in a Bacteroides hybrid two-component system.
Heparin / metabolism. Heparitin Sulfate / metabolism. Humans. Intestines / metabolism, microbiology. Models, Biological. Models ... Here, we identify the activating ligand of the hybrid two-component system, BT4663, which controls heparin and heparan sulfate ... 0/Bacterial Proteins; 9005-49-6/Heparin; 9050-30-0/Heparitin Sulfate; EC 2.7.-/Protein Kinases ...http://www.biomedsearch.com/nih/scissor-blade-like-closing-mechanism/22532667.html
Structure of Foot-and-mouth disease virus serotype A10 61 alone and complexed with oligosaccharide receptor: receptor...
... has an RGSD motif in place of the otherwise conserved RGD integrin-binding motif and the potential to bind heparan sulphate ( ... Heparin bound at a similar site and in a similar conformation to that seen in the analogous complex with O(1)BFS, although the ... Crystal structures are available for type O, A and C viruses and also for a complex of type O strain O(1)BFS with heparin. The ... structure of FMDV A10(61) (a cell culture-adapted strain) complexed with heparin has now been determined. This virus ...https://www.neuroscience.ox.ac.uk/publications/27469
Patente US4840941 - Method for inhibiting infection of human T-cells - Google Patentes
... chitosan sulfate, chondroitin polysulfates, further sulfated chondroitin-4-sulfate and -6-sulfate, heparin, etc. having S- ... keratan sulfate, chondroitin-4-sulfate, chondroitin-6-sulfate, dermatan sulfate, teichuronic acid, hyaluronic acid, heparitin ... heparan sulfate from bovine liver or lung, and chitin from arthropod or from fungus or yeast, respectively. The process for ... 100 100Chondroitin-6-sulfate polysulfate 15 2 1Heprin 7,000-3,000 13 41 1Heparitin sulfate 15,000 7 100 90Keratan sulfate 4,000 ...http://www.google.es/patents/US4840941?dq=flatulence
Addition of a C-terminal cysteine improves the anti-herpes simplex virus activity of a peptide containing the human...
TAT-C bound to heparan sulfate but was a poor competitor for viral attachment. The antiviral activity depended on the net ... Anti-HSV activity of lactoferricin analogues is only partly related to their affinity for heparan sulfate.. *Håvard Jenssen, ... AntiHSV activity of lactoferrin and lactoferricin is dependent on the presence of heparin sulfphate at the cell surface. ... Extended Release of an Anti-Heparan Sulfate Peptide From a Contact Lens Suppresses Corneal Herpes Simplex Virus-1 Infection. * ...https://www.semanticscholar.org/paper/Addition-of-a-C-terminal-cysteine-improves-the-of-a-Bultmann-Teuton/6ab39f39bf261330c29a411176b33411730f5914
Salt overload damages the glycocalyx sodium barrier of vascular endothelium - Semantic Scholar
Quantitative eGC analyses reveal that sodium overload caused a reduction of heparan sulphate residues by 68% which lead to ... Inhibition of inflammation induced shedding of the endothelial glycocalyx with low molecular weight heparin.. *Herbert H ... Heparitin Sulfate. *Drug abuse. *Endothelial Cells. *Phenobarbital. *Fluid overload. *In Vitro [Publication Type] ...https://www.semanticscholar.org/paper/Salt-overload-damages-the-glycocalyx-sodium-barrier-Oberleithner-Peters/d75fbd21881bc8f889dba4eeb1342c94f066db98
Loss of heparan sulfate glycosaminoglycan assembly in podocytes does not lead to proteinuria.
Podocytes synthesize the majority of the glomerular basement membrane components with some contribution from the glomerular capillary endothelial cells. The anionic charge of heparan sulfate proteoglycans is conferred by covalently attached heparan sulfate glycosaminoglycans and these are thought to provide critical charge selectivity to the glomerular basement membrane for ultrafiltration. One key component in herparan sulfate glycosaminoglycan assembly is the Ext1 gene product encoding a subunit of heparan sulfate co-polymerase. Here we knocked out Ext1 gene expression in podocytes halting polymerization of heparin sulfate glycosaminoglycans on the proteoglycan core proteins secreted by podocytes. Glomerular development occurred normally in these knockout animals but ...http://repository.ubn.ru.nl/handle/2066/69069
The heparin/heparan sulfate sequence that interacts with cyclophilin B contains a 3-O-sulfated N-unsubstituted glucosamine...
Many of the biological functions of heparan sulfate (HS) proteoglycans can be attributed to specialized structures within HS moieties, which are thought to modulate binding and function of various effector proteins. Cyclophilin B (CyPB), which was initially identified as a cyclosporin A-binding protein, triggers migration and integrin-mediated adhesion of peripheral blood T lymphocytes by a mechanism dependent on interaction with cell surface HS. Here we determined the structural features of HS that are responsible for the specific binding of CyPB. In addition to the involvement of 2-O,6-O, and N-sulfate groups, we also demonstrated that binding of CyPB was dependent on the presence of N-unsubstituted glucosamine residues (GlcNH2), which have been reported to be precursors for sulfation by 3-O-sulfotransferases-3 (3-OST-3). Interestingly, 3-OST-3B isoform was found to be the main 3-OST isoenzyme expressed in peripheral blood ...http://christie.openrepository.com/christie/handle/10541/71358
國立交通大學機構典藏：Synthetic heparin and heparan sulfate oligosaccharides and their protein interactions
Heparin and heparan sulfate bind a host of basic proteins that take advantage of the sugar's dense structural information. The significance of these interactions in various aspects of development, physiology, and disease stimulated keen interest in evaluating structure-activity relationships. The well-defined heparin and heparan sulfate oligosaccharides needed for these studies can be mainly accessed by chemical synthesis and, more recently by chemoenzymatic means. The various synthetic strategies available to chemical synthesis have recently enabled the acquisition of several regular and irregular sequences, including a number of dodecasaccharides, through improved coupling methods and judicial protecting group manipulations. Controlled chain elongation and critical application of modification enzymes allowed the generation of well-defined constructs via chemoenzymatie ...https://ir.nctu.edu.tw/handle/11536/23654
Altered glycosylation of recombinant NKp30 hampers binding to heparan sulfate: a lesson for the use of recombinant...
NKp30 is a natural cytotoxicity receptor expressed by human NK cells and involved in NK lytic activity. We previously published that membranal heparan sulfate serves as a coligand for human NKp30. In the present study, we complement our results by showing direct binding of recombinant NKp30 to immobilized heparin. The heparan sulfate epitope(s) on target tumor cells and the heparin epitope(s) recognized by NKp30 share similar characteristics. Warren and colleagues (Warren HS, Jones AL, Freeman C, Bettadapura J, Parish CR. 2005. Evidence that the cellular ligand for the human NK cell activation receptor NKp30 is not a heparan sulfate glycosaminoglycan. J Immunol. 175:207-212) published that NKp30 does not bind to membranal heparan sulfate on target cells and that ...http://christie.openrepository.com/christie/handle/10541/56020
Regulation of FGF-1 mitogenic activity by heparan sulfate oligosaccharides is dependent on specific structural features:...
The interaction of heparan sulfate (HS) (and the closely related molecule heparin) with FGF-1 is a requirement for enabling the growth factor to activate its cell surface tyrosine kinase receptor. However, little is known about the regulatory role of naturally occurring cell surface HS in FGF-1 activation. We have addressed this issue by utilizing a library of HS oligosaccharides, which are defined in both length and sulfate content. Mitogenic activation assays using these oligosaccharides showed that HS contained both FGF-1 activatory and inhibitory sugar sequences. Further analysis of these oligosaccharides showed a clear correlation between FGF-1 promoting activity and their 6-O-sulfate content. The results, in particular with the dodecasaccharide sequences, suggested that specific positioning of 6-O-sulfate groups may be required for the ...http://eprints.hud.ac.uk/id/eprint/2268/
The Heparan and Heparin Metabolism Pathway is Involved in Regulation of Fatty Acid Composition
Research has shown that the enzymes and proteins encoded by DSEL (dermatan sulfate epimerase-like), EXTL1 (exostoses (multiple)-like 1), HS6ST1 (heparan sulfate 6-O-sulfotransferase 1), HS6ST3 (heparan sulfate 6-O-sulfotransferase 3), NDST3 (N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3), and SULT1A1 (sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1), are involved in heparan sulfate and heparin metabolism . Both heparan sulfate and heparin are members of the glycosaminoglycan family of carbohydrates that are very closely related in structure. As reviewed by Kolset and Salmivirta , cell surface heparan sulfate proteoglycans play biological ...http://www.ijbs.com/v07p0659.htm
Heparan Sulfate Proteoglycan 2抗体[A74]参考文献
引用Abcam Anti-Heparan Sulfate Proteoglycan 2抗体[A74]的参考文献列表。为您列举引用本产品的发表文章，并提供信息包括论文文献数据库中的检索编号以便您搜寻文章。http://www.abcam.cn/heparan-sulfate-proteoglycan-2-antibody-a74-ab23418-references.html
A cyclic anti-heparan sulfate peptide provides protection against primary and recurrent herpes simplex virus-1 (HSV-1)...
Purpose : Primary and recurrent ocular herpes infections affect almost 60-90% of the population and in severe cases is one of the leading causes of infectious blindness. This study describes the topical use of a cyclic anti-heparan sulfate peptide that shows promise in blocking ocular herpes infections. Methods : Cyclic peptide synthesis and confirmation was performed at the Protein Research Laboratory at the University of Illinois at Chicago. A random cyclic peptide sequence was also generated to serve as a control. Cell viability assay via MTT was performed in human corneal epithelial cells (HCE) to rule out non-toxic concentrations. HSV-1 virus entry using a recombinant virus strain encoding the beta-galactosidase gene and viral protein synthesis and replication of HSV-1 were assayed in the presence of the control and treatment cyclic peptides. Using the mouse corneas as a model of ocular infection, the therapeutic activity of the cyclic peptide was ...http://iovs.arvojournals.org/article.aspx?articleid=2641397
"Basement membrane proteoglycans: modulators Par Excellence of cancer g" by Renato V. Iozzo, Jason J. Zoeller et al.
Proteoglycans located in basement membranes, the nanostructures underling epithelial and endothelial layers, are unique in several respects. They are usually large, elongated molecules with a collage of domains that share structural and functional homology with numerous extracellular matrix proteins, growth factors and surface receptors. They mainly carry heparan sulfate side chains and these contribute not only to storing and preserving the biological activity of various heparan sulfate-binding cytokines and growth factors, but also in presenting them in a more 'active configuration' to their cognate receptors. Abnormal expression or deregulated function of these proteoglycans affect cancer and angiogenesis, and are critical for the evolution of the tumor microenvironment. This review will focus on the functional roles of the major heparan sulfate ...http://jdc.jefferson.edu/pacbfp/26/
The expression of heparan sulfate on dendritic spines | Open-i
The expression of heparan sulfate on dendritic spines of cultured hippocampal neurons. (A-E) Double immunostaining of hippocampal neurons at 30 DIV with anhttps://openi.nlm.nih.gov/detailedresult.php?img=PMC2132915_JCB9810074.f3&req=4
Localization of the Cdc42Hs-binding site of IRS-58. Dif | Open-i
Localization of the Cdc42Hs-binding site of IRS-58. Different portions of IRS-58 were expressed as GST fusion proteins and either dot-blotted or Western-transfehttps://openi.nlm.nih.gov/detailedresult.php?img=PMC2195994_JCB0011075.f3ad&req=4
Overexpression of heparan sulfate 6-O-sulfotransferases in human embryonic kidney 293 cells results in increased N-acetyl...
Heparan sulfate (HS), a linear negatively charged polysaccharide located at the cell surface and in the extracellular matrix, interacts with, and thereby regulates the functions of numerous proteins. HS-protein interactions depend on the fine structure of HS, especially its sulfation pattern. This thesis aimed to understand how differently sulfated domains in HS are generated. Specifically, the substrate specificities of HS hexuronic acid 2-O-sulfotransferase (2OST) and HS glucosaminyl 6-O-sulfotransferases (6OSTs) were investigated. Three different 6OSTs (6OST1-3) have been cloned and characterized. To study the mechanisms controlling 6-O-sulfation we incubated the recombinant purified 6-OST isoforms with different 6-O-desulfated poly- and oligosaccharide substrates and the active sulfate donor 3'-phosphoadenosine ...http://uu.diva-portal.org/smash/record.jsf?pid=diva2:165615
Kuberan Balagurunathan - | University of Utah
Peer-Reviewed Publications and Book Chapters. "Discovery of novel sulfonated small molecules that inhibit vascular tube formation", K. Raman, R. Karuturi, V.P. Swarup, U.R. Desai, B. Kuberan, Bioorg Med Chem Lett, 22, 4467-4470, 2012. "A synthetic heparan sulfate oligosaccharide library reveals the novel enzymatic action of D-glucosaminyl 3-O-sulfotransferase-3a", T. K. Nguyen, S. Arungundram, V.M. Tran, K. Raman, K. Al-Mafraji, A. Venot, G. J. Boons, B. Kuberan, Mol Biosyst, 8, 609-614, 2012. " Is N-sulfation just a gateway modification during heparan sulfate biosynthesis?", Karthik Raman, Thao Kim Nu Nguyen, B. Kuberan, FEBS Letters, 585, 3420-3423, 2011. "Investigating the Mechanism of the Assembly of FGF1-binding heparan sulfate motifs", Thao Kim Nu Nguyen, Karthik Raman, Vy My Tran, B. Kuberan, FEBS Letters, 585, 2698-2702, 2011. "Novel ...http://pharmacy.utah.edu/medchem/faculty/current-faculty/Kuberan%20B.php
Test for heparan sulfate functionality?: post #1
Test for heparan sulfate functionality? - posted in Protein and Proteomics: Hi! I'm wondering if there is an assay that can test for heparan sulfate binding function. I'm not that familiar with protein interactions studies, I primarily work with virology and cell biology. I have tested heparan sulfate and it's effect on our virus ability to bind to cells, and we have found that it has no effect. What I now need is a way to show that the heparan sulfate is actually working i...http://www.protocol-online.org/forums/topic/28381-test-for-heparan-sulfate-functionality/
KEGG PATHWAY: Glycosaminoglycan biosynthesis - heparan sulfate / heparin - Reference pathway + Vertebrates
Heparan sulfate (HS) and heparin (Hep) are glycosaminoglycans with repeating disaccharide units that consist of alternating residues of alpha-D-glucosamine (GlcN) and uronic acid, the latter being either beta-D-glucuronic acid (GlcA) or alpha-L-iduronic acid (IdoA). In these sugar residues, sulfation modification may be performed at various positions. Structural studies show that Hep possesses a higher degree of sulfation than HS. The biosynthesis of HS/Hep occurs with the addition of the first GlcNAc residue by EXTL3 glycosyltransferase after completion of tetrasaccharide linkage region attached to serine residue of a core protein. The chain polymeraization is then catalyzed by EXT1 and EXT2 transferases. As the chain polymerizes, HS/Hep undergoes a series of modification reactions including N-deacetylation, N-sulfation, epimerization, and subsequently O-sulfation. As final products of biosynthesis, HS is present in the form of hepran ...http://www.genome.jp/kegg-bin/show_pathway?category=Vertebrates&mapno=00534
Heparan sulfate 2-O-sulfotransferase 1
Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. This gene encodes a member of the heparan sulfate biosynthetic enzyme family that transfers sulfate to the 2 position of the iduronic acid residue of heparan sulfate. The disruption of this gene resulted in no kidney formation in knockout embryonic mice, indicating that the absence of this enzyme may interfere with the signaling required for kidney formation. Two alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Aug 2008 ...https://pharos.nih.gov/idg/targets/Q7LGA3
JCI - Series Introduction: Heparan sulfate proteoglycans: intricate molecules with intriguing functions
The Perspectives in this and following issues update our understanding of HSPG biology in various areas of basic research and clinical medicine, encompassing cancer; angiogenesis; tumor invasion; hereditary disorders, both induced and uninduced; and viral diseases. Before considering the role of HSPGs in disease states, it is essential to have a good grasp of the biosynthetic pathways leading to the formation of the complex heparan sulfate. Esko and Lindahl provide an overview of heparan sulfate structure and biosynthesis and set the stage for discussing how alterations in this fascinating class of compounds can lead to human disorders. Understanding heparan structure and biosynthesis would also assist in understanding the potential implication of abrogating specific enzymes involved in its biosynthesis and modifications. The nearly mathematical perfection of heparan ...https://www.jci.org/articles/view/13560
DSpace@MIT: Heparan sulfate glycosaminoglycan regulation of vasculogenesis
Neovascularization is an essential process to repair ischemic tissues following myocardial infarction, stroke, diabetic complications, or transplant procedures. Blood vessels are generated by distinct vasculogenic and angiogenic processes. Although multiple proangiogenic factors have been identified, limited success has been achieved translating these as clinical therapeutics. Furthermore, recent studies have shown that vasculogenesis contributes to adult neovascularization in multiple settings. Harnessing the vasculogenic potential of embryonic stem cells is an emerging concept to generate neovasculature. The differentiation of embryonic stem cells into endothelium has been well documented, however most studies focus on genetic or chemokine regulation. Limited information exists which implicates the role of the extracellular microenvironment in stem cell differentiation. Heparan sulfate glycosaminoglycans (HSGAG) are a crucial part of the dynamic ...http://dspace.mit.edu/handle/1721.1/63084
Endostatin is recognized by heparan sulfate epitopes containing both sulfated and acetylated N-substituents
Heparan sulfate (HS) is a polysaccharide of glycosaminoglycan type composed of alternating hexuronic acid [either glucuronic acid (GlcA) or iduronic acid (IdoA)] and glucosamine (GlcN) units that can be sulfated in various positions. HS binds to a large number of proteins and these interactions promote many biological processes, including cell adhesion and growth factor signaling. This thesis deals with the structural analysis of short heparan sulfate sequences that mediate binding to fibroblast growth factors FGF1 and FGF2, their receptor FGFR4, and the angiogenesis inhibitor endostatin.. Both FGF1 and FGF2 were shown to interact with N-sulfated hexa- and octasaccharide fragments isolated from HS. A pool of HS fragments depleted for FGF1 binding retained the ability to bind FGF2. Changes in 6-O sulfation affected binding to FGF1 but not ...http://uu.diva-portal.org/smash/record.jsf?pid=diva2:161078
Patente US20040013653 - Stimulation of angiogenesis via enhanced endothelial expression of syndecan ... - Google Patentes
The present invention provides tangible means and methods for stimulation of angiogenesis via enhanced endothelial expression of core proteins having a syndecan-4 cytoplasmic region intracellularly. The tangible means include a prepared DNA sequence fragment having separate and individual DNA sequenced portions coding for an heparan sulfate binding extracellular domain, a central transmembrane domain, and a cytoplasmic domain coding for the syndecan-4 polypeptide. The prepared DNA sequence unitary fragment may be delivered to endothelial cells in-situ, both under in-vivo and/or in-vitro conditions, using suitable expression vectors including plasmids and viruses. The resulting transfected endothelial cells overexpress heparan sulfate binding, core proteins; and the resulting overexpression of these proteoglycan entities causes stimulation of angiogenesis in-situ.http://www.google.es/patents/US20040013653
Snapping Up Sulfate | Science
Sulfate-binding proteins hold their ligands by means of a large number of neutral hydrogen-bonding interactions, but designers of small synthetic receptors generally have had to resort to electrostatic interactions to capture the sulfate ion. Bondy et al. have modified a system for binding sulfate that relies on Pt2+ centers (which provide an electrostatic interaction) that sport four nicotinamide arms (which form hydrogen bonds). Now, by using iso-quinoline ligands derivatized with urea groups, they can stabilize sulfate binding in deuterated dimethyl sulfoxide (DMSO-d6) enough so that they can follow anion binding on the nuclear magnetic resonance time scale. Halides formed 2:1 complexes with these receptors, but the four urea moieties converged on a single sulfate ion in a 1:1 complex featuring eight hydrogen bonds, as revealed by x-ray ...http://science.sciencemag.org/content/304/5669/361.3
CiNii 論文 - Elimination of heparan sulfate by heparitinases induces abnormal mesodermal and...
Yamada Shuhei , Sugahara Kazuyuki Trends in glycoscience and glycotechnology 10(52), 95-123, 1998-03-02 医中誌Web 参考文献154件 被引用文献6件 ...https://ci.nii.ac.jp/naid/10008755943
Gentaur Molecular :Biovis \ G418 Sulfate; Appearance White to off-white solid \ 1557-5G
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3,5-tetrahydroaldosterone sulfate - THAS Summary Report | CureHunter
3,5-tetrahydroaldosterone sulfate: RN given refers to (3beta,5beta,11beta)-isomer; RN for cpd without isomeric designation not available 9/90http://www.curehunter.com/public/keywordSummaryC065339-3-5-tetrahydroaldosterone-sulfate-THAS.do