US4721096A - Process for replicating bone marrow in vitro and using the same - Google Patents
... and then infusing the replicated bone marrow cells into a person whose bone marrow has been destroyed or functionally ... Further, when grown in culture, hematopoietic stem cells are relatively non-adherent. This non-adherence of the hematopoietic ... A61K35/28-Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells ... 106 cells. After incubation for 1 day, the non-adherent cells are removed by washing with culture medium and the adherent cells ...https://patents.google.com/patent/US4721096
Pharmaceuticals | Free Full-Text | Stem Cell Therapy: A New Treatment for Burns? | HTML
... embryonic and adult stem cells) or artificially obtained (induced pluripotent stem cells or iPSCs) and their fates have become ... The aim of this review is to describe well established characteristics of stem cells and to delineate new advances in the stem ... We are at the beginning of a new era in medicine, with multiple applications for stem cell therapy, not only as a monotherapy, ... Severe burns have several indications for stem cell therapy, including enhancement of wound healing, replacement of damaged ...http://www.mdpi.com/1424-8247/4/10/1355/htm
Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection | Retrovirology | Full Text
HD cells were generated by differentiating clonally expanded cultures of adherent subsets of ASCs (CD90+, CD105+, CD45-, and ... Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection. ... Characterization of hematopoietic potential of mesenchymal stem cells. J Cell Physiol. 2010, 225: 888-97.View ArticlePubMed ... hematopoietic progenitor cells are commonly known to generate macrophages. Furthermore, the ability of embryonic stem cells to ...https://retrovirology.biomedcentral.com/articles/10.1186/1742-4690-8-3
The development of macrophages from human CD34+ haematopoietic stem cells in serum-free cultures is optimized by IL-3 and SCF. ...
... the effect on cell proliferation and differentiation to adherent macrophage-like cells was determined. The proliferative ... such as CD34+ haematopoietic stem cells, can further inform with regard to the development of macrophage-lineage cells. CD34+ ... response of CD34+ cells to M-CSF was determined during the initial phase of cell culture. Thirteen combinations of SCF, IL-3, ... cells were cultured in serum-free medium containing Flt3L, SCF, IL-3, IL-6 and M-CSF. Using differing combinations of growth ...https://www.kennedy.ox.ac.uk/publications/375416
Preferential expansion of human umbilical cord blood-derived CD34-positive cells on major histocompatibility complex-matched...
... hematopoietic stem cell-proliferation) and long-term culture-initiating cell assays, greater expansion of the Lin-CD34+ cells ... Results Culture-expanded amnion-derived adherent cells expressed mesenchymal stem cell markers and HLA-ABC molecules and could ... a MHC restriction exists between hematopoietic stem cells and mesenchymal stem cells/stromal cells in the human hematopoietic ... and contained a large number of hematopoietic stem cells. When the Lin-CD34+ cells were cultured on the autologous (MHC-matched ...http://www.haematologica.org/content/94/5/618
Patente US9005965 - Stem cells culture systems - Google Patentes
Further provided are cultures of feeder cells for use in stem cell technology, as well as cultures, culture systems and methods ... propagating of stem cells in an undifferentiated state as well as for the development of somatic cells cultures from stem cells ... the somatic cell cultures being free of extraembryonic cells. ... Provided are systems and methods for providing human cell ... of an adherent cell feeder layer from human umbilical cord blood for support of long-term hematopoietic progenitor cell growth ...http://www.google.es/patents/US9005965
CiNii 論文 - Murine mesenchymal stem cells isolated by low density primary culture system ...
... of the adherent stromal cells and their interactions in maintaining the proliferation of hematopoietic stem cells TAVASSOLI M. ... Removal of hematopoietic cells and macrophage from mouse bone marrow culture : isolation of fibroblastic stromal cells ... Mesenchymal stem cells home to injured tissues when co-infused with hematopoietic cells to treat a radiation-induced multi- ... Murine mesenchymal stem cells isolated by low density primary culture system * * Eslaminejad Mohamadreza Baghaban ESLAMINEJAD ...https://ci.nii.ac.jp/naid/10018212392
Current Perspectives in Mesenchymal Stem Cell Therapies for Osteoarthritis
... and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and ... Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, ... Under normal culture conditions, MSCs display a fibroblast-like morphology, are adherent to plastic, and can form colonies from ... while lacking the expression of the haematopoietic antigens CD11b, CD14, CD34, CD45, CD79, CD19, and HLA-DR . In order to ...https://www.hindawi.com/journals/sci/2014/194318/
A New Human Somatic Stem Cell from Placental Cord Blood with Intrinsic Pluripotent Differentiation Potential | JEM
1, A and B , USSCs are adherent, spindle-shaped cells, and have a size of 20-25 μm. Hematopoietic CD45+ cells were no longer ... Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J. Cell. Biochem. 64:295-312. ... the stem cell compartment in cord blood (CB) is less mature. This has been documented for the hematopoietic stem cells, which ... Transplantation of fetal hematopoietic stem cells in utero: the creation of hematopoietic chimeras. Science. 233:776-778. ...http://jem.rupress.org/content/200/2/123
Pluristem Granted U.S. Patent for Production Methods and Use of Placental Cell Therapy in a Range of Indications | Latest News
Nasdaq:PSTI) (TASE:PSTI), a leading developer of placenta-based cell therapy products, today announced that the U. S. ... The patent protects specific methods of culturing placental adherent stromal cells, and use of these cells for the treatment of ... intermittent claudication), graft-versus-host disease, solid organ transplantation, hematopoietic stem cell transplantation, ... Trademark Office has issued to the Company patent #9,096,827 titled "Adherent Cells From Placenta Tissue and Use Thereof in ...http://www.biomedreports.com/articles/latest-news/233556-pluristem-granted-us-patent-for-production-methods-and-use-of-placental-cell-therapy-in-a-range-of-indications.html?tmpl=component&print=1&layout=default&page=
Unrestricted somatic stem cells from human umbilical cord blood grow in serum-free medium as spheres | BMC Biotechnology | Full...
... spheres cultured in stem cell proliferation media grow as an adherent monolayer. (Magnification, ×200; scale bar is 100 μm). ... Although USSCs are rare compared to haematopoietic stem cells in cord blood, they can be expanded rapidly to yield large ... Oct4 and its pseudogenes confuse stem cell research. Cell Stem Cell. 2007, 1: 364-366. 10.1016/j.stem.2007.09.003.View Article ... Cells were plated at 7 × 103 cells/cm2 cultured for ten days in stem cell proliferation medium containing 10-7 M dexamethasone ...https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-9-101
Mesenchymal stem cell - Wikipedia
Mesenchymal stem cells, but not red blood cells or haematopoetic progenitors, are adherent to tissue culture plastic within 24 ... These bone marrow stem cells do not contribute to the formation of blood cells so do not express the hematopoietic stem cell ... Mesenchymal stem cells are characterized morphologically by a small cell body with a few cell processes that are long and thin ... These stem cells have been shown capable of producing hepatocytes. Stem cells have been identified in amniotic fluid. As many ...https://en.wikipedia.org/wiki/Mesenchymal_stem_cell
Vascular Progenitors From Cord Blood-Derived Induced Pluripotent Stem Cells Possess Augmented Capacity for Regenerating...
... for generating therapeutic patient-specific hemangioblasts and hematopoietic stem cells from human pluripotent stem cells. Int ... of the hemogenic endothelial progenitor and its direct precursor in human pluripotent stem cell differentiation cultures. Cell ... and subdivided adherent CD31+ cells by their coexpression of the endothelial-perivascular-mesenchymal stem/progenitor marker ... human embryonic stem cell; hPSC, human pluripotent stem cell; iPSC, induced pluripotent stem cell; LDM, liquid differentiation ...http://circ.ahajournals.org/content/129/3/359
Supportive Interaction Between Cell Survival Signaling and Angiocompetent Factors Enhances Donor Cell Survival and Promotes...
Nonadherent hematopoietic cells were removed during routine fresh medium replacement. The adherent, spindle-shaped MSCs were ... Mesenchymal stem cells can be differentiated into endothelial cells in vitro. Stem Cells. 2004; 22: 377-384. ... Ang-1-specific ELISA was performed on the cell culture supernatant samples collected from MAAs, using cell culture supernatant ... BM cells were seeded into 150-mm dishes and cultured in DMEM supplemented with 15% FBS and antibiotics. A small number of cells ...http://circres.ahajournals.org/content/99/7/776
Endothelial progenitor cells in non-Hodgkin's lymphoma | Haematologica
2002) Vascular and haematopoietic stem cells: novel targets for anti-angiogenesis therapy? Nat Rev Cancer 2:826-35. ... a population of postnatal blood cells that formed an adherent layer of cells of endothelial morphology after 7 days of culture ... 2005) Stem cell research and cell transplantation for myocardial regeneration. Eur J Cardiothorac Surg 28:318-24. ... In cancer, angiogenic factors can be produced by the tumor cells themselves but also by surrounding hematopoietic cells in the ...http://www.haematologica.org/content/92/4/433
Computers Software & Internet « Blog
If you did not know Stemcell.com focuses on 6 specialized cell culture media. Embryonic stem cells & iPS cells, STEMCELL ... Mesenchymal stem cells are plastic-adherent, fibroblast-like cells which are able to self-renew and differentiate into bone, ... Hematopoietic stem & progenitor cells can be found in a variety of different tissues through development notably the bone ... expansion and differentiation of human and mouse mesenchymal stem cells. Neural stem & progenitor cells STEMCELL Technologies ...http://sunpride2000.com/category/computers-software-internet/
Isolation and Expansion of Endothelial Progenitor Cells Derived from Mouse Embryonic Stem Cells
... suspension culture. The most convenient and efficient of these is suspension culture of ESCs in non-adherent plates (3, 4). ... hematopoietic, and epithelial cells, emerges. Only a subset of these cells will differentiate toward endothelial cells (less ... Pipette the cells up and down with a 5 mL cell culture pipette and wash the plate to detach all the cells (use a cell scraper ... Endothelial differentiation of embryonic stem cells. Curr Protoc Stem Cell Biol. 2008;Chapter 1(Unit 1) F 5. [PMC free article] ...http://pubmedcentralcanada.ca/pmcc/articles/PMC3733326/?lang=en-ca
C166 ATCC ® CRL-2581™ Mus musculus The C166 cell line was e
Mus musculus The C166 cell line was e ... stable proliferation of multipotential hematopoietic stem cells ... Culture Properties adherent Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is ... In vitro and in vivo differentiation into B cells, T cells, and myeloid cells of primitive yolk sac hematopoietic precursor ... In vitro and in vivo differentiation into B cells, T cells, and myeloid cells of primitive yolk sac hematopoietic precursor ...https://www.atcc.org/en/Products/All/CRL-2581.aspx
Allen C Eaves - Wikipedia
Functional characterization of individual human hematopoietic stem cells cultured at limiting dilution on supportive marrow ... Unregulated proliferation of primitive chronic myeloid leukemic progenitors in the presence of normal marrow adherent cells. ... 2016 James Till Ernest McCulloch Connie Eaves Stem Cells Hematopoiesis Hematopoietic stem cells Cellular therapy Bone marrow ... Rapid decline of chronic myeloid leukemic cells in long-term culture due to a defect at the leukemic stem cell level. Proc Natl ...https://en.wikipedia.org/wiki/Allen_C_Eaves
Protocols and Video Articles Authored by Gillian Farnie
Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures' ... Many groups have extrapolated the cancer stem cell hypothesis from the haematopoietic system to solid cancers, where using in ... Using a non-adherent sphere culture technique we have grown DCIS mammospheres from primary DCIS tissue, where self-renewal ... Mammary Stem Cells and Breast Cancer--role of Notch Signalling Stem Cell Reviews. Jun, 2007 , Pubmed ID: 17873349 Adult stem ...https://www.jove.com/author/Gillian_Farnie
Stem Cells: History, Concepts and Characterization
... in the stromal adherent fraction of the bone marrow sustaining the homeostatic turnover of non-haematopoietic stromal cells, ... Studies in isolation and culture of stem cells started since 1964, when Kleinsmith and Pierce developed conditions to culture ... Mesenchymal Stem Cells (MSCs) are a type of adult stem cells widely studied and an attractive stem cell source for clinical ... DISCLAIMER: The use of stem cells or stem cell rich tissues as well as the mobilization of stem cells by any means, e.g., ...https://eternamdregenerativemedicine.com/technhical-blogs/stem-cells-history-concepts-and-characterization/
Phenotypical and functional characteristics of mesenchymal stem cells from bone marrow: comparison of culture using different...
Phenotypic characteristics of mesenchymal stem cells cultured in various expansion media. Morphological analysis. Adherent cell ... like hematopoietic supportive stromal cells [1, 2]. These cells are capable of multilineage differentiation from a single cell ... at a cell concentration of 1 × 105 cells per square centimeter for BM cells. Cells were cultured in basic growth medium (BGM) ... Mesenchymal stem cell expansion cultures from bone marrow. BM cells (n = 13) were isolated from patients with normal BMs ...https://stemcellres.biomedcentral.com/articles/10.1186/scrt97
Mesenchymal stem cells in pathogenesis of myelodysplastic syndromes
... looking at adherent cell layers in Dexter-type long-term cultures indicated that the composition and function of such cells ... 2. Nimer SD.. MDS: a stem cell disorder--but what exactly is wrong with the primitive hematopoietic cells in this disease? ... Myelodysplastic cells in patients reprogram mesenchymal stromal cells to establish a transplantable stem cell niche disease ... Raaijmakers MH.. Disease progression in myelodysplastic syndromes: do mesenchymal cells pave the way? Cell Stem Cell 2014;14: ...http://pubmedcentralcanada.ca/pmcc/articles/PMC4923510/
Animal Cell Culture: Introduction, Types, Methods and Applications - microbeonline
Hematopoietic stem cells (derived from blood, spleen and bone marrow) and tumor cells can be grown in suspension. These cells ... These cells are also called anchorage independent or non-adherent cells which can be grown floating in the culture medium. ... Cell lines can undergo finite or infinite cell divisions.. Animal cell culture. A. Primary cell culture. This is the cell ... B. Secondary cell culture and cell line. When a primary culture is sub-cultured, it is known as secondary culture or cell line ...http://microbeonline.com/animal-cell-culture-introduction-types-methods-applications/
Vascular Trauma Induces Rapid but Transient Mobilization of VEGFR2+AC133+ Endothelial Precursor Cells | Circulation Research
... a novel hematopoietic stem-cell marker, is also expressed on subsets of CD34+ cells.7 AC133+ CEPs express vascular endothelial ... vWF+-adherent colonies that formed from passaged nonadherent cells, between 6 to 8 weeks of initial culture, were scored as ... between CEPs and mature ECs or hematopoietic cells is complicated by the fact that subsets of hematopoietic cells express ... cells have the capacity to form late-outgrowth colonies and also express the stem-cell marker AC133, they can be considered as ...http://circres.ahajournals.org/content/88/2/167
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