Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell...
MΦ in culture promote CXCL12 production. (A) Morphology of adherent layer of Dexter culture 24 h after addition of PBS or ... Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell ... Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell ... Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell ...https://www.ncbi.nlm.nih.gov/pubmed/?term=Bone+marrow+CD169%2B+macrophages+promote+the+retention+of+hematopoietic+stem+and+progenitor+cells+in+the+mesenchymal+stem+cell+niche
Indirect CD133 MicroBead Kit, human - Hematopoietic stem cells - MicroBeads and Isolation Kits - Cell separation reagents -...
Neural progenitor cells can be isolated from single-cell suspensions from primary neural tissues or cell lines. ES and iPS cell ... or hematopoietic progenitors can be isolated from differentiated ES or iPS cell cultures1 . Cancer stem cells can be isolated ... Hematopoietic stem cells can be isolated from peripheral blood, cord blood, bone marrow, or leukapheresis product. ... from single-cell suspensions from primary tumor tissue or cell lines. ...https://www.miltenyibiotec.com/BE-en/products/macs-cell-separation/cell-separation-reagents/microbeads-and-isolation-kits/hematopoietic-stem-cells/indirect-cd133-microbead-kit-human.html
US4721096A - Process for replicating bone marrow in vitro and using the same - Google Patents
... and then infusing the replicated bone marrow cells into a person whose bone marrow has been destroyed or functionally ... Further, when grown in culture, hematopoietic stem cells are relatively non-adherent. This non-adherence of the hematopoietic ... A61K35/28-Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells ... 106 cells. After incubation for 1 day, the non-adherent cells are removed by washing with culture medium and the adherent cells ...https://patents.google.com/patent/US4721096
Pharmaceuticals | Free Full-Text | Stem Cell Therapy: A New Treatment for Burns? | HTML
... embryonic and adult stem cells) or artificially obtained (induced pluripotent stem cells or iPSCs) and their fates have become ... The aim of this review is to describe well established characteristics of stem cells and to delineate new advances in the stem ... We are at the beginning of a new era in medicine, with multiple applications for stem cell therapy, not only as a monotherapy, ... Severe burns have several indications for stem cell therapy, including enhancement of wound healing, replacement of damaged ...http://www.mdpi.com/1424-8247/4/10/1355/htm
Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection | Retrovirology | Full Text
HD cells were generated by differentiating clonally expanded cultures of adherent subsets of ASCs (CD90+, CD105+, CD45-, and ... self-renewing cells known for their differentiation potential into cells of mesenchymal lineage. The ability of single cell ... In the present study, we investigated if the hematopoietic differentiated (HD) cells derived from ASCs could productively be ... HIV-1 infection increased apoptosis and cell cycle regulatory genes in HD cells. Although undifferentiated ASCs failed to show ...https://retrovirology.biomedcentral.com/articles/10.1186/1742-4690-8-3
The development of macrophages from human CD34+ haematopoietic stem cells in serum-free cultures is optimized by IL-3 and SCF. ...
... the effect on cell proliferation and differentiation to adherent macrophage-like cells was determined. The proliferative ... such as CD34+ haematopoietic stem cells, can further inform with regard to the development of macrophage-lineage cells. CD34+ ... response of CD34+ cells to M-CSF was determined during the initial phase of cell culture. Thirteen combinations of SCF, IL-3, ... cells were cultured in serum-free medium containing Flt3L, SCF, IL-3, IL-6 and M-CSF. Using differing combinations of growth ...https://www.kennedy.ox.ac.uk/publications/375416
Preferential expansion of human umbilical cord blood-derived CD34-positive cells on major histocompatibility complex-matched...
... hematopoietic stem cell-proliferation) and long-term culture-initiating cell assays, greater expansion of the Lin-CD34+ cells ... Results Culture-expanded amnion-derived adherent cells expressed mesenchymal stem cell markers and HLA-ABC molecules and could ... a MHC restriction exists between hematopoietic stem cells and mesenchymal stem cells/stromal cells in the human hematopoietic ... and contained a large number of hematopoietic stem cells. When the Lin-CD34+ cells were cultured on the autologous (MHC-matched ...http://www.haematologica.org/content/94/5/618
Patente US9005965 - Stem cells culture systems - Google Patentes
Further provided are cultures of feeder cells for use in stem cell technology, as well as cultures, culture systems and methods ... propagating of stem cells in an undifferentiated state as well as for the development of somatic cells cultures from stem cells ... the somatic cell cultures being free of extraembryonic cells. ... Provided are systems and methods for providing human cell ... of an adherent cell feeder layer from human umbilical cord blood for support of long-term hematopoietic progenitor cell growth ...http://www.google.es/patents/US9005965
CiNii 論文 - Murine mesenchymal stem cells isolated by low density primary culture system ...
... of the adherent stromal cells and their interactions in maintaining the proliferation of hematopoietic stem cells TAVASSOLI M. ... Removal of hematopoietic cells and macrophage from mouse bone marrow culture : isolation of fibroblastic stromal cells ... Mesenchymal stem cells home to injured tissues when co-infused with hematopoietic cells to treat a radiation-induced multi- ... Murine mesenchymal stem cells isolated by low density primary culture system * * Eslaminejad Mohamadreza Baghaban ESLAMINEJAD ...https://ci.nii.ac.jp/naid/10018212392
Current Perspectives in Mesenchymal Stem Cell Therapies for Osteoarthritis
... and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and ... Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, ... Under normal culture conditions, MSCs display a fibroblast-like morphology, are adherent to plastic, and can form colonies from ... while lacking the expression of the haematopoietic antigens CD11b, CD14, CD34, CD45, CD79, CD19, and HLA-DR . In order to ...https://www.hindawi.com/journals/sci/2014/194318/
A New Human Somatic Stem Cell from Placental Cord Blood with Intrinsic Pluripotent Differentiation Potential | JEM
1, A and B , USSCs are adherent, spindle-shaped cells, and have a size of 20-25 μm. Hematopoietic CD45+ cells were no longer ... Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J. Cell. Biochem. 64:295-312. ... the stem cell compartment in cord blood (CB) is less mature. This has been documented for the hematopoietic stem cells, which ... Transplantation of fetal hematopoietic stem cells in utero: the creation of hematopoietic chimeras. Science. 233:776-778. ...http://jem.rupress.org/content/200/2/123
Pluristem Granted U.S. Patent for Production Methods and Use of Placental Cell Therapy in a Range of Indications | Latest News
Nasdaq:PSTI) (TASE:PSTI), a leading developer of placenta-based cell therapy products, today announced that the U. S. ... The patent protects specific methods of culturing placental adherent stromal cells, and use of these cells for the treatment of ... intermittent claudication), graft-versus-host disease, solid organ transplantation, hematopoietic stem cell transplantation, ... Trademark Office has issued to the Company patent #9,096,827 titled "Adherent Cells From Placenta Tissue and Use Thereof in ...http://www.biomedreports.com/articles/latest-news/233556-pluristem-granted-us-patent-for-production-methods-and-use-of-placental-cell-therapy-in-a-range-of-indications.html?tmpl=component&print=1&layout=default&page=
Mesenchymal stem cells (human) - Human cell types - Human cells and organs - MACS Handbook - Resources - Miltenyi Biotec
plastic-adherent growth when maintained under standard culture conditions. *differentiation to osteoblasts, adipocytes, and ... Dendritic cells, monocytes, macrophages NK cells Cancer Pluripotent stem cells Hematopoietic stem cells Mesenchymal stem cells ... these cells showed non-hematopoietic differentiation potential. Eventually, the cells became known as mesenchymal stem cells or ... T cell products B cell products NK cell products DCs, monocytes, macrophage products Granulocyte products Cancer products Stem ...https://www.miltenyibiotec.com/BE-en/resources/macs-handbook/human-cells-and-organs/human-cell-types/mesenchymal-stem-cells-human.html
Flow Cytometry and Cellular Imaging Core Facility - Services & Fees - North Campus | MD Anderson Cancer Center
Hematopoietic progenitor and stem cell sorting (e.g. CD34+/38-, CD34+/38+, CD34+38-123+, Lin-/Sca1+/ c-kit+, Hoechst 33342 SP ... FACS sorted, adherent or rare tumor or stem cells can be analyzed using this technology. Protein expression data can be ... analysis is complemented by the ability to isolate any desired cell population with high-speed cell sorting for culture and ... cell proliferation and differentiation, cell signaling pathways, enzyme activity, gene regulation, cell lineage, apoptosis, ...https://www.mdanderson.org/research/research-resources/core-facilities/flow-cytometry-and-cellular-imaging-core-facility/services-and-fees-north-campus.html
Mesenchymal stem cell - Wikipedia
Mesenchymal stem cells, but not red blood cells or haematopoetic progenitors, are adherent to tissue culture plastic within 24 ... These bone marrow stem cells do not contribute to the formation of blood cells so do not express the hematopoietic stem cell ... Mesenchymal stem cells are characterized morphologically by a small cell body with a few cell processes that are long and thin ... These stem cells have been shown capable of producing hepatocytes. Stem cells have been identified in amniotic fluid. As many ...https://en.wikipedia.org/wiki/Mesenchymal_stem_cell
Unrestricted somatic stem cells from human umbilical cord blood grow in serum-free medium as spheres | BMC Biotechnology | Full...
... spheres cultured in stem cell proliferation media grow as an adherent monolayer. (Magnification, ×200; scale bar is 100 μm). ... Although USSCs are rare compared to haematopoietic stem cells in cord blood, they can be expanded rapidly to yield large ... Oct4 and its pseudogenes confuse stem cell research. Cell Stem Cell. 2007, 1: 364-366. 10.1016/j.stem.2007.09.003.View Article ... Cells were plated at 7 × 103 cells/cm2 cultured for ten days in stem cell proliferation medium containing 10-7 M dexamethasone ...https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-9-101
Long-Term Therapeutic Effects of Mesenchymal Stem Cells Compared to Dexamethasone on Recurrent Experimental Autoimmune Uveitis...
Nonadherent cells were collected as T cells, while the adherent cells were removed from columns after incubation on ice, and ... 104 cell/well), in a total volume of 200 μL. For crossover experiments, T cells from control groups were cultured in the ... Further characterization was based on the expression of CD90 and CD29, and the absence of cell surface hematopoietic markers, ... Mesenchymal stem cell effects on T-cell effector pathways. Stem Cell Res Ther . 2011; 11: 34-42. [CrossRef] ...https://iovs.arvojournals.org/article.aspx?articleid=2166518
Vascular Progenitors From Cord Blood-Derived Induced Pluripotent Stem Cells Possess Augmented Capacity for Regenerating...
... for generating therapeutic patient-specific hemangioblasts and hematopoietic stem cells from human pluripotent stem cells. Int ... of the hemogenic endothelial progenitor and its direct precursor in human pluripotent stem cell differentiation cultures. Cell ... and subdivided adherent CD31+ cells by their coexpression of the endothelial-perivascular-mesenchymal stem/progenitor marker ... human embryonic stem cell; hPSC, human pluripotent stem cell; iPSC, induced pluripotent stem cell; LDM, liquid differentiation ...http://circ.ahajournals.org/content/129/3/359
Supportive Interaction Between Cell Survival Signaling and Angiocompetent Factors Enhances Donor Cell Survival and Promotes...
Nonadherent hematopoietic cells were removed during routine fresh medium replacement. The adherent, spindle-shaped MSCs were ... Mesenchymal stem cells can be differentiated into endothelial cells in vitro. Stem Cells. 2004; 22: 377-384. ... Ang-1-specific ELISA was performed on the cell culture supernatant samples collected from MAAs, using cell culture supernatant ... BM cells were seeded into 150-mm dishes and cultured in DMEM supplemented with 15% FBS and antibiotics. A small number of cells ...http://circres.ahajournals.org/content/99/7/776
Endothelial progenitor cells in non-Hodgkin's lymphoma | Haematologica
2002) Vascular and haematopoietic stem cells: novel targets for anti-angiogenesis therapy? Nat Rev Cancer 2:826-35. ... a population of postnatal blood cells that formed an adherent layer of cells of endothelial morphology after 7 days of culture ... 2005) Stem cell research and cell transplantation for myocardial regeneration. Eur J Cardiothorac Surg 28:318-24. ... In cancer, angiogenic factors can be produced by the tumor cells themselves but also by surrounding hematopoietic cells in the ...http://www.haematologica.org/content/92/4/433
Computers Software & Internet « Blog
If you did not know Stemcell.com focuses on 6 specialized cell culture media. Embryonic stem cells & iPS cells, STEMCELL ... Mesenchymal stem cells are plastic-adherent, fibroblast-like cells which are able to self-renew and differentiate into bone, ... Hematopoietic stem & progenitor cells can be found in a variety of different tissues through development notably the bone ... expansion and differentiation of human and mouse mesenchymal stem cells. Neural stem & progenitor cells STEMCELL Technologies ...http://sunpride2000.com/category/computers-software-internet/
Isolation and Expansion of Endothelial Progenitor Cells Derived from Mouse Embryonic Stem Cells
... suspension culture. The most convenient and efficient of these is suspension culture of ESCs in non-adherent plates (3, 4). ... hematopoietic, and epithelial cells, emerges. Only a subset of these cells will differentiate toward endothelial cells (less ... Pipette the cells up and down with a 5 mL cell culture pipette and wash the plate to detach all the cells (use a cell scraper ... Endothelial differentiation of embryonic stem cells. Curr Protoc Stem Cell Biol. 2008;Chapter 1(Unit 1) F 5. [PMC free article] ...http://pubmedcentralcanada.ca/pmcc/articles/PMC3733326/?lang=en-ca
C166 ATCC ® CRL-2581™ Mus musculus The C166 cell line was e
Mus musculus The C166 cell line was e ... stable proliferation of multipotential hematopoietic stem cells ... Culture Properties adherent Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is ... In vitro and in vivo differentiation into B cells, T cells, and myeloid cells of primitive yolk sac hematopoietic precursor ... In vitro and in vivo differentiation into B cells, T cells, and myeloid cells of primitive yolk sac hematopoietic precursor ...https://www.atcc.org/en/Products/All/CRL-2581.aspx
Allen C Eaves - Wikipedia
Functional characterization of individual human hematopoietic stem cells cultured at limiting dilution on supportive marrow ... Unregulated proliferation of primitive chronic myeloid leukemic progenitors in the presence of normal marrow adherent cells. ... 2016 James Till Ernest McCulloch Connie Eaves Stem Cells Hematopoiesis Hematopoietic stem cells Cellular therapy Bone marrow ... Rapid decline of chronic myeloid leukemic cells in long-term culture due to a defect at the leukemic stem cell level. Proc Natl ...https://en.wikipedia.org/wiki/Allen_C_Eaves
Protocols and Video Articles Authored by Gillian Farnie
Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures' ... Many groups have extrapolated the cancer stem cell hypothesis from the haematopoietic system to solid cancers, where using in ... Using a non-adherent sphere culture technique we have grown DCIS mammospheres from primary DCIS tissue, where self-renewal ... Mammary Stem Cells and Breast Cancer--role of Notch Signalling Stem Cell Reviews. Jun, 2007 , Pubmed ID: 17873349 Adult stem ...https://www.jove.com/author/Gillian_Farnie