Umbilical Cord-Derived Mesenchymal Stem Cells for Hematopoietic Stem Cell Transplantation
They proliferate as fibroblastic spindle-shaped cells and must be plastic-adherent when maintained in standard culture ... "Rapid hematopoietic recovery after coinfusion of autologous-blood stem cells and culture-expanded marrow mesenchymal stem cells ... "Cotransplantation of HLA-identical sibling culture-expanded mesenchymal stem cells and hematopoietic stem cells in hematologic ... Umbilical Cord-Derived Mesenchymal Stem Cells for Hematopoietic Stem Cell Transplantation. Yu-Hua Chao,1,2 Han-Ping Wu,3 Chin- ...https://www.hindawi.com/journals/bmri/2012/759503/
Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell...
MΦ in culture promote CXCL12 production. (A) Morphology of adherent layer of Dexter culture 24 h after addition of PBS or ... Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell ... Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell ... Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell ...https://www.ncbi.nlm.nih.gov/pubmed/?term=Bone+marrow+CD169%2B+macrophages+promote+the+retention+of+hematopoietic+stem+and+progenitor+cells+in+the+mesenchymal+stem+cell+niche
Cotransplantation of Marrow Stromal Cells May Prevent Lethal Graft-versus-Host Disease in Major Histocompatibility Complex...
Marrow stromal cells (MSC) produce a microenvironment supporting hematopoiesis and may contribute immune tolerance because of ... Marrow stromal cell Allogeneic hematopoietic stem cell transplantation Cell proliferation assay Interferon-γ ... Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage, and lung in irradiated mice ... Phenotypic and functional comparison of cultures of marrow- derived mesenchymal stem cell (MSCs) and stromal cells. J Cell ...https://link.springer.com/article/10.1532/IJH97.A30409
Indirect CD133 MicroBead Kit, human - Hematopoietic stem cells - MicroBeads and Isolation Kits - Cell separation reagents -...
Neural progenitor cells can be isolated from single-cell suspensions from primary neural tissues or cell lines. ES and iPS cell ... or hematopoietic progenitors can be isolated from differentiated ES or iPS cell cultures1 . Cancer stem cells can be isolated ... Hematopoietic stem cells can be isolated from peripheral blood, cord blood, bone marrow, or leukapheresis product. ... from single-cell suspensions from primary tumor tissue or cell lines. ...https://www.miltenyibiotec.com/BE-en/products/macs-cell-separation/cell-separation-reagents/microbeads-and-isolation-kits/hematopoietic-stem-cells/indirect-cd133-microbead-kit-human.html
US4721096A - Process for replicating bone marrow in vitro and using the same - Google Patents
... and then infusing the replicated bone marrow cells into a person whose bone marrow has been destroyed or functionally ... Further, when grown in culture, hematopoietic stem cells are relatively non-adherent. This non-adherence of the hematopoietic ... A61K35/28-Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells ... 106 cells. After incubation for 1 day, the non-adherent cells are removed by washing with culture medium and the adherent cells ...https://patents.google.com/patent/US4721096
Pharmaceuticals | Free Full-Text | Stem Cell Therapy: A New Treatment for Burns? | HTML
... embryonic and adult stem cells) or artificially obtained (induced pluripotent stem cells or iPSCs) and their fates have become ... The aim of this review is to describe well established characteristics of stem cells and to delineate new advances in the stem ... We are at the beginning of a new era in medicine, with multiple applications for stem cell therapy, not only as a monotherapy, ... Severe burns have several indications for stem cell therapy, including enhancement of wound healing, replacement of damaged ...http://www.mdpi.com/1424-8247/4/10/1355/htm
Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection | Retrovirology | Full Text
HD cells were generated by differentiating clonally expanded cultures of adherent subsets of ASCs (CD90+, CD105+, CD45-, and ... self-renewing cells known for their differentiation potential into cells of mesenchymal lineage. The ability of single cell ... In the present study, we investigated if the hematopoietic differentiated (HD) cells derived from ASCs could productively be ... HIV-1 infection increased apoptosis and cell cycle regulatory genes in HD cells. Although undifferentiated ASCs failed to show ...https://retrovirology.biomedcentral.com/articles/10.1186/1742-4690-8-3
The development of macrophages from human CD34+ haematopoietic stem cells in serum-free cultures is optimized by IL-3 and SCF. ...
... the effect on cell proliferation and differentiation to adherent macrophage-like cells was determined. The proliferative ... such as CD34+ haematopoietic stem cells, can further inform with regard to the development of macrophage-lineage cells. CD34+ ... response of CD34+ cells to M-CSF was determined during the initial phase of cell culture. Thirteen combinations of SCF, IL-3, ... cells were cultured in serum-free medium containing Flt3L, SCF, IL-3, IL-6 and M-CSF. Using differing combinations of growth ...https://www.kennedy.ox.ac.uk/publications/375416
Preferential expansion of human umbilical cord blood-derived CD34-positive cells on major histocompatibility complex-matched...
... hematopoietic stem cell-proliferation) and long-term culture-initiating cell assays, greater expansion of the Lin-CD34+ cells ... Results Culture-expanded amnion-derived adherent cells expressed mesenchymal stem cell markers and HLA-ABC molecules and could ... a MHC restriction exists between hematopoietic stem cells and mesenchymal stem cells/stromal cells in the human hematopoietic ... and contained a large number of hematopoietic stem cells. When the Lin-CD34+ cells were cultured on the autologous (MHC-matched ...http://www.haematologica.org/content/94/5/618
US10087408B2 - System and method for microfluidic cell culture - Google Patents
... on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to ... Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational ... primary hematopoietic stem cells; hybridoma cells or primary blood-born cancer cells. Suspension cells may be hematopoietic in ... adherent or weakly adherent cells may be cultured as suspension cells under appropriate conditions. Suspension cells may ...https://patents.google.com/patent/US10087408B2
Patente US9005965 - Stem cells culture systems - Google Patentes
Further provided are cultures of feeder cells for use in stem cell technology, as well as cultures, culture systems and methods ... propagating of stem cells in an undifferentiated state as well as for the development of somatic cells cultures from stem cells ... the somatic cell cultures being free of extraembryonic cells. ... Provided are systems and methods for providing human cell ... of an adherent cell feeder layer from human umbilical cord blood for support of long-term hematopoietic progenitor cell growth ...http://www.google.es/patents/US9005965
Hematopoietic Stem and Progenitor Cells (HSPCs)
Blood cells are produced by the proliferation and differentiation of a very small population of pluripotent hema ... div class='section', ,h2,Introduction,/h2, ,p,Mature blood cells have a finite life-span and must be continuously replaced ... performed by culturing hematopoietic cells on an adherent monolayer of primary stromal cells or on immortalized stromal cell ... Culture of Hematopoietic Cells. Hematopoietic cells can be stimulated to proliferate in culture using many different methods. ...https://www.stemcell.com/hematopoietic-stem-and-progenitor-cells-lp.html
CA2624916A1 - Production of oligodendrocytes from placenta-derived stem cells - Google Patents
The present invention provides methods and compositions for the production of glial cells and oligodendrocytes from placenta ... stem cells. The invention further provides for the use of these glia and oligodendrocytes in the treatment of, and intervention ... Cells within the embryoid-like bodies are typically not adherent to the culture substrate, as are the placental stem cells ... an isolated population of placental stem cells is combined with a plurality of hematopoietic stem cells. Such hematopoietic ...https://patents.google.com/patent/CA2624916A1/en
CiNii 論文 - Murine mesenchymal stem cells isolated by low density primary culture system ...
... of the adherent stromal cells and their interactions in maintaining the proliferation of hematopoietic stem cells TAVASSOLI M. ... Removal of hematopoietic cells and macrophage from mouse bone marrow culture : isolation of fibroblastic stromal cells ... Mesenchymal stem cells home to injured tissues when co-infused with hematopoietic cells to treat a radiation-induced multi- ... Murine mesenchymal stem cells isolated by low density primary culture system * * Eslaminejad Mohamadreza Baghaban ESLAMINEJAD ...https://ci.nii.ac.jp/naid/10018212392
Mesenchymal stem cells improve mouse non-heart-beating liver graft survival by inhibiting Kupffer cell apoptosis via TLR4-ERK1...
All bone marrow cells were cultured in DMEM with 10% foetal bovine serum (FBS; GIBCO) for 48 h. Non-adherent haematopoietic ... Mesenchymal stem cell inhibition of T-helper 17 cell- differentiation is triggered by cell-cell contact and mediated by ... we used an in vitro cell-cell co-culture system with the murine-derived RAW264.7 cell line to represent Kupffer cells, which ... Mesenchymal stem cells: a new strategy for immunosuppression and tissue repair. Cell Res. 2010;20:510-8.CrossRefPubMedGoogle ...https://rd.springer.com/article/10.1186/s13287-016-0416-y
Current Perspectives in Mesenchymal Stem Cell Therapies for Osteoarthritis
... and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and ... Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, ... Under normal culture conditions, MSCs display a fibroblast-like morphology, are adherent to plastic, and can form colonies from ... while lacking the expression of the haematopoietic antigens CD11b, CD14, CD34, CD45, CD79, CD19, and HLA-DR . In order to ...https://www.hindawi.com/journals/sci/2014/194318/
Cellular Kinetics of Perivascular MSC Precursors
... and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and ... Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, ... harbors multiple types of stem/progenitor cells, including hematopoietic stem cells (HSCs), endothelial progenitor cells (EPCs ... "Mesenchymal stem cells: a perspective from in vitro cultures to in vivo migration and niches," European Cells and Materials, ...https://www.hindawi.com/journals/sci/2013/983059/
Mesenchymal stem cells from cryopreserved human umbilical cord blood. - Semantic Scholar
... able to obtain homogeneous plastic adherent cells from the mononuclear cell fractions of cryopreserved UCB using our culture ... is well known to be a rich source of hematopoietic stem cells with practical and ethical advantages, but the presence of ... These adherent cell populations exhibited fibroblast-like morphology and typical mesenchymal-like immunophenotypes (CD73+, ... CD105+, and CD166+, etc.). These cells presented the self-renewal capacity and the mesenchymal cell-lineage potential to form ...https://www.semanticscholar.org/paper/Mesenchymal-stem-cells-from-cryopreserved-human-Lee-Choi/4b4eab2550cb2950c6e07ab89797ea33634c1edb
A New Human Somatic Stem Cell from Placental Cord Blood with Intrinsic Pluripotent Differentiation Potential | JEM
1, A and B , USSCs are adherent, spindle-shaped cells, and have a size of 20-25 μm. Hematopoietic CD45+ cells were no longer ... Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J. Cell. Biochem. 64:295-312. ... the stem cell compartment in cord blood (CB) is less mature. This has been documented for the hematopoietic stem cells, which ... Transplantation of fetal hematopoietic stem cells in utero: the creation of hematopoietic chimeras. Science. 233:776-778. ...http://jem.rupress.org/content/200/2/123
Compact disc164 is a cell adhesion molecule that boosts hematopoietic stem | Selective Inhibitors of Protein Methyltransferases
... we cultured H2122 and CL 1-5 cells under stem cell development moderate in 96-well plates via the restricting dilution method. ... The forming of spheroid cells was discovered produced H2122 and CL 1-5 adherent cells after 14 days (Amount ?(Figure8A).8A). ... Compact disc164 is a cell adhesion molecule that boosts hematopoietic stem. Compact disc164 is a cell adhesion molecule that ... C. Cell viability of BEAS2BCD164 cells, BEAS2BWT cells, and BEAS2BVeh cells had been analyzed from the MTT assay. The outcomes ...http://onetownmanyvoices.com/compact-disc164-is-a-cell-adhesion-molecule-that-boosts-hematopoietic-stem/
Pluristem Granted U.S. Patent for Production Methods and Use of Placental Cell Therapy in a Range of Indications | Latest News
Nasdaq:PSTI) (TASE:PSTI), a leading developer of placenta-based cell therapy products, today announced that the U. S. ... The patent protects specific methods of culturing placental adherent stromal cells, and use of these cells for the treatment of ... intermittent claudication), graft-versus-host disease, solid organ transplantation, hematopoietic stem cell transplantation, ... Trademark Office has issued to the Company patent #9,096,827 titled "Adherent Cells From Placenta Tissue and Use Thereof in ...http://www.biomedreports.com/articles/latest-news/233556-pluristem-granted-us-patent-for-production-methods-and-use-of-placental-cell-therapy-in-a-range-of-indications.html?tmpl=component&print=1&layout=default&page=
Mesenchymal stem cells (human) - Human cell types - Human cells and organs - MACS Handbook - Resources - Miltenyi Biotec
plastic-adherent growth when maintained under standard culture conditions. *differentiation to osteoblasts, adipocytes, and ... Dendritic cells, monocytes, macrophages NK cells Cancer Pluripotent stem cells Hematopoietic stem cells Mesenchymal stem cells ... these cells showed non-hematopoietic differentiation potential. Eventually, the cells became known as mesenchymal stem cells or ... T cell products B cell products NK cell products DCs, monocytes, macrophage products Granulocyte products Cancer products Stem ...https://www.miltenyibiotec.com/BE-en/resources/macs-handbook/human-cells-and-organs/human-cell-types/mesenchymal-stem-cells-human.html
Pluristem Granted U.S. Patent for Production Methods and Use of Placental Cell Therapy in a Range of Indications
17, 2015-- Pluristem Therapeutics Inc., a leading developer of placenta-based cell therapy products, today announced that the U ... The patent protects specific methods of culturing placental adherent stromal cells, and use of these cells for the treatment of ... hematopoietic stem cell transplantation, diabetes, autoimmune diseases, neuropathic pain and cancer.. "The U.S. is a very ... "Adherent Cells From Placenta Tissue and Use Thereof in Therapy".. ...https://www.cnbc.com/2015/08/17/globe-newswire-pluristem-granted-us-patent-for-production-methods-and-use-of-placental-cell-therapy-in-a-range-of-indications.html
Mesenchymal stem cell - Wikipedia
Mesenchymal stem cells, but not red blood cells or haematopoetic progenitors, are adherent to tissue culture plastic within 24 ... These bone marrow stem cells do not contribute to the formation of blood cells so do not express the hematopoietic stem cell ... Mesenchymal stem cells are characterized morphologically by a small cell body with a few cell processes that are long and thin ... These stem cells have been shown capable of producing hepatocytes. Stem cells have been identified in amniotic fluid. As many ...https://en.wikipedia.org/wiki/Mesenchymal_stem_cell
Flow Cytometry and Cellular Imaging Core Facility - Services & Fees - North Campus | MD Anderson Cancer Center
Hematopoietic progenitor and stem cell sorting (e.g. CD34+/38-, CD34+/38+, CD34+38-123+, Lin-/Sca1+/ c-kit+, Hoechst 33342 SP ... FACS sorted, adherent or rare tumor or stem cells can be analyzed using this technology. Protein expression data can be ... analysis is complemented by the ability to isolate any desired cell population with high-speed cell sorting for culture and ... cell proliferation and differentiation, cell signaling pathways, enzyme activity, gene regulation, cell lineage, apoptosis, ...https://www.mdanderson.org/research/research-resources/core-facilities/flow-cytometry-and-cellular-imaging-core-facility/services-and-fees-north-campus.html