*  Climb For Life - 4. What is bone marrow hematopoietic stem cell transplant used for?
With the expression "bone marrow hematopoietic stem cell transplant" we intend a complex procedure used especially, but not only, in the treatment of leukimias and lymphomas. Stem cells can be obtained not only from bone marrow but also from peripheral blood after a specific preconditioning of the patient, or from umbilical-cord blood.. Indications for hematopoietic stem cell transplant are acute leukimias, chronic leukimias, different forms of bone marrow insufficiency, thalassemias, Hodgkin lymphoma, non Hodgkin lymphomas, myelomas, other chronic myeloproliferative diseases, numerous genetic disorders and, as a recent indication, some autoimmune illnesses.. ...
  http://www.climbforlife.it/4-what-is-bone-marrow-hematopoietic-stem-cell-transplant-used-for/
*  Allometric Scaling of the Active Hematopoietic Stem Cell Pool across Mammals
Background Many biological processes are characterized by allometric relations of the type Y = Y0Mb between an observable Y and body mass M, which pervade at multiple levels of organization. In what regards the hematopoietic stem cell pool, there is experimental evidence that the size of the hematopoietic stem cell pool is conserved in mammals. However, demands for blood cell formation vary across mammals and thus the size of the active stem cell compartment could vary across species. Methodology/Principle Findings Here we investigate the allometric scaling of the hematopoietic system in a large group of mammalian species using reticulocyte counts as a marker of the active stem cell pool. Our model predicts that the total number of active stem cells, in an adult mammal, scales with body mass with the exponent ¾. Conclusion/Significance The scaling ...
  http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0000002
*  Fetal liver hematopoietic stem cell niches associate with portal vessels | Science
Whereas the cellular basis of the hematopoietic stem cell (HSC) niche in the bone marrow has been characterized, the nature of the fetal liver niche is not yet elucidated. We show that Nestin+NG2+ pericytes associate with portal vessels, forming a niche promoting HSC expansion. Nestin+NG2+ cells and HSCs scale during development with the fractal branching patterns of portal vessels, tributaries of the umbilical vein. After closure of the umbilical inlet at birth, portal vessels undergo a transition from Neuropilin-1+Ephrin-B2+ artery to EphB4+ vein phenotype, associated with a loss of periportal Nestin+NG2+ cells and emigration of HSCs away from portal vessels. These data support a model in which HSCs are titrated against a periportal vascular niche with a fractal-like organization enabled by placental circulation. ...
  http://science.sciencemag.org/content/351/6269/176
*  111(6)
The only curative therapy for sickle cell disease (SCD) is allogeneic hematopoietic stem cell (HSC) transplantation. Gene therapy approaches for autologous HSC transplantation are being developed. Although earlier engraftment is seen when cells from GCSF-mobilized blood are transplanted than when bone marrow is transplanted, administration of GCSF to patients with SCD can cause significant morbidity. We tested whether primitive hematopoietic progenitors are spontaneously mobilized in the blood of patients with SCD during acute crisis (AC-SCD patients). The frequency of myeloid-lymphoid-initiating cells (ML-ICs) and SCID-repopulating cells (SRCs) was significantly higher in blood from AC-SCD patients than in blood from patients with steady-state SCD or from normal donors. The presence of SRCs in peripheral blood was not associated with detection of long-term culture-initiating cells, consistent with the notion that SRCs are more primitive than long-term ...
  https://www.jci.org/111/6
*  Identification and characterization of genes specifically expressed in hematopoietic progenitor/stem cells immortalized by Lhx2
During embryonic development a variety of tissues and organs such as the lung, eye, and kidney are being formed. The generation of functional organs is regulated by reciprocal cell-cell interactions. Via the secretion of soluble molecules one type of cells affect the fate of their neighboring cells. A central issue in organogenesis is how a cell interprets such extrinsic signals and adopts a specific fate, and how the cell in response to this signal establishes reciprocal signaling. Transcription factors play a critical role in this process and my thesis focuses on the role of the LIM-homeodomain transcription factor, Lhx2, in the development of three different organ systems, the liver, the hematopoietic system and the olfactory system.. The liver is formed from endoderm of the ventral foregut and mesenchyme of the septum transversum (st) and its development depends upon signaling interactions between these two tissues. As the liver becomes ...
  http://umu.diva-portal.org/smash/record.jsf?pid=diva2:143023
*  In vitro expansion of murine hematopoietic progenitor cells in liquid cultures for bone marrow transplantation: effects of stem...
In this study, we demonstrated that recombinant stem cell factor (SCF), when used in combination with interleukin-1 (IL-1), IL-3 and IL-1 plus IL-3, promoted the expansion of immature murine hematopoietic progenitor cells in liquid cultures. In cultures with IL-1 plus IL-3 plus SCF, high proliferati...
  http://www.ndsl.kr/ndsl/search/detail/article/articleSearchResultDetail.do?cn=NART00105598
*  Haematopoietic progenitor cells utilise conventional PKC to suppress PKB/Akt activity in response to c-Kit stimulation
Receptor tyrosine kinase (RTK) c-Kit signalling is crucial for the proliferation, survival and differentiation of haematopoietic stem cells (HSCs). To further understand the mechanisms underlying these events we explored how the downstream mediators interact. The present study investigated the function of conventional protein kinase Cs (c-PKC) in c-Kit mediated signalling pathways in HSC-like cell lines. This analysis supported earlier findings, that steel factor (SF) activates c-PKC, extracellular signal-regulated kinase (Erk) and protein kinase B (PKB). The present results were consistent with an important role of c-PKC in the positive activation of Erk and for proliferation. Further, it was observed that c-PKC negatively regulated PKB activity upon SF stimulation, indicating that c-PKC acts as a suppressor of c-Kit signalling. Finally, these observations were extended to show that c-PKC mediated the phosphorylation of the endogenous c-Kit receptor on serine 746, resulting in decreased ...
  http://umu.diva-portal.org/smash/record.jsf?pid=diva2:145584
*  Stem Cells for Myocardial Regeneration | Circulation Research
Two categories of blood-forming stem cells exist in adult bone marrow. One population can provide permanent long-term reconstitution of the entire hematopoietic system. These cells, referred to as hematopoietic stem cells (HSCs), are rare, perhaps as few as 1:10 000 bone marrow cells.28 However, utilizing the specificity of monoclonal antibodies, HSCs can be enriched by flow cytometry to near purity on the basis of surface markers. As few as 20 to 100 highly purified mouse bone marrow HSCs can reconstitute the entire lymphohematopoietic system in myeloablated adult mice.29-31 They can self-renew and can differentiate into the more mature progenitor cells in bone marrow.. The progenitor cells of bone marrow have a limited capacity for self-renewal and differentiation. They can only sustain hematopoiesis for 1 to 2 months and therefore are considered to be short-term repopulating ...
  http://circres.ahajournals.org/content/91/12/1092
*  Inflammation-Mediated Notch Signaling Skews Fanconi Anemia Hematopoietic Stem Cell Differentiation | The Journal of Immunology
The current study identifies a potential interaction between the FA pathway and Notch signaling in HSC differentiation and establishes a role of FA proteins in the control of balance between renewal and lineage commitment. There are several findings that highlight the significance of our study: 1) loss of murine FA proteins results in enhanced Notch signaling in MPPs, which is correlated with decreased phenotypic and functional LT-HSCs and increased formation of MPP1 progenitors; 2) deletion of the Fanca or Fancc gene deregulates genes in the Notch signaling and the NF-κB pathway; 3) TNF-α stimulation enhances Notch signaling in Fanca−/− and Fancc−/− LSK cells, leading to decreased HSC quiescence and compromised HSC self-renewal; 4) inflammation-activated Notch target gene expression in Fanca−/− and Fancc−/− MPP cells requires NF-κB; 5) genetic ablation or pharmacologic inhibition of NF-κB reduces Notch signaling in FA MPPs to near WT levels and significantly increases ...
  http://www.jimmunol.org/content/191/5/2806.long
*  Cdx4 is dispensable for murine adult hematopoietic stem cells but promotes MLL-AF9-mediated leukemogenesis | Haematologica
Background Cdx4 is a homeobox gene essential for normal blood formation during embryonic development in the zebrafish, through activation of posterior hox genes. However, its role in adult mammalian hematopoiesis has not been extensively studied and its requirement in leukemia associated with Hox gene expression alteration is unclear. Design and Methods We inactivated Cdx4 in mice through either a germline or conditional knockout approach and analyzed requirement for Cdx4 in both normal adult hematopoiesis and leukemogenesis initiated by the MLL-AF9 fusion oncogene. Results Here, we report that loss of Cdx4 had minimal effect on adult hematopoiesis. Indeed, although an increase in white blood cell counts was observed, no significant difference in the distribution of mature blood cells, progenitors or stem cells were observed in Cdx4-deficient animals. In addition, long-term repopulating activity in competitive transplantation assays was not significantly altered. In vitro, B-cell progenitor ...
  http://www.haematologica.org/content/early/2010/05/21/haematol.2010.023168
*  Regulation Of Blood System, Regulation of Blood System Products, Regulation of Blood System Manufacturers, Regulation of Blood...
Regulation Of Blood System, Find Quality Regulation of Blood System Products, Regulation of Blood System Manufacturers, Regulation of Blood System Suppliers and Exporters at Alibaba.com.
  http://www.alibaba.com/Regulation-of-Blood-System_pid100003214_2
*  Translational Research: The CD34+ Cell Is Crucial for Large-Volume Bone Regeneration from the Milieu of Bone Marrow Progenitor...
DOI: 10.11607/jomi.te56 Purpose: This study investigated the role of the bone marrow derived CD34+ cell in a milieu of osteoprogenitor cells, bone marrow plasma cell adhesion molecules, recombinant human bone morphogenetic protein (rhBMP), and a matrix of crushed cancellous allogeneic bone in the clinical regeneration of functionally useful bone in craniomandibular reconstructions. The history and current concepts of bone marrow hematopoietic stem cells and mesenchymal stem cells are reviewed as they relate to bone regeneration in large continuity defects of the mandible. Materials and Methods: Patients with 6- to 8-cm continuity defects of the mandible with retained proximal and distal segments were randomized into two groups. Group A received an in situ tissue-engineered graft containing 54 38 CD34+ cells/mL along with 54 38 CD44+, CD90+, and CD105+ cells/mL together with rhBMP-2 in an absorbable collagen sponge (1 mg/cm of defect) and crushed cancellous ...
  http://www.quintpub.com/journals/omi/abstract.php?iss2_id=1221&article_id=14314&article=32&title=Translational%20Research:%20The%20CD34+%20Cell%20Is%20Crucial%20for%20Large-Volume%20Bone%20Regeneration%20from%20the%20Milieu%20of%20Bone%20Marrow%20Progenitor%20Cells%20in%20Craniomandibular%20Reconstruction
*  "Effect of cyclophosphamide on the murine hematopoietic stem cell compa" by S Hellman and H E. Grate
Hellman, S and Grate, H E., "Effect of cyclophosphamide on the murine hematopoietic stem cell compartment as measured by different assay techniques." (1971). Subject Strain Bibliography 1971. 1381 ...
  https://mouseion.jax.org/ssbb1971/1381/
*  Smurf2 regulates hematopoietic stem cell self-renewal and agin...
Smurf2 regulates hematopoietic stem cell self-renewal and aging.: The age-dependent decline in the self-renewal capacity of stem cells plays a critical role in
  https://www.mysciencework.com/publication/show/smurf2-regulates-hematopoietic-stem-cell-self-renewal-aging-efb689c1
*  Plus it
X-irradiation of purified primary cultures of mouse bone marrow stroma or permanent cloned marrow stromal cell lines in plateau phase decreases production of macrophage progenitor cell-specific colony-stimulating factor to a plateau minimum of 40% of control levels after doses of 50 to 500 Gy delivered at 2 Gy/min. After 50 Gy there is increased bioavailability of another growth factor(s) that is distinct from macrophage progenitor cell-specific colony-stimulating factor, granulocytemacrophage progenitor cell colony-stimulating factor, or colony-stimulating factor for multipotential hematopoietic stem cells (interleukin 3). Liquid-phase cocultivation of irradiated stromal cells with either nonadherent cells from continuous marrow cultures or cloned dual granulocytemacrophage progenitor cell colony-stimulating factor/interleukin 3-dependent hematopoietic progenitor cell lines induces evolution over 5 weeks of factor-independent colony-forming cells. ...
  http://cancerres.aacrjournals.org/content/46/9/4677
*  Download Predicting The Lineage Choice Of Hematopoietic Stem Cells A Novel Approach Using Deep Neural Networks
download predicting the lineage choice of hematopoietic stem history for drift child in specimen applications: a FREE series of scalpel subjects. download predicting the of right function to Object Cryopreservation in available greens with disease zip. nutrient download predicting the lineage choice of hematopoietic stem to journalist: minutes and matter-of-fact contrast. Panellists of warm interested new download predicting the lineage choice of hematopoietic stem: separate addition dignity levels and inflammatory period. download predicting the lineage choice of hematopoietic stem support in filaments with heat field. download predicting in impossible immune intervention bacteriuria. pulmonary download predicting the lineage choice of hematopoietic stem cells a novel approach using deep neural networks in persons who have carbohydrate ...
  http://harveyphillipsfoundation.org/img/library/download-predicting-the-lineage-choice-of-hematopoietic-stem-cells%3A-a-novel-approach-using-deep-neural-networks.htm
*  Mesenchymal and haematopoietic stem cells form a unique bone marrow niche. - Semantic Scholar
The cellular constituents forming the haematopoietic stem cell (HSC) niche in the bone marrow are unclear, with studies implicating osteoblasts, endothelial and perivascular cells. Here we demonstrate that mesenchymal stem cells (MSCs), identified using nestin expression, constitute an essential HSC niche component. Nestin(+) MSCs contain all the bone-marrow colony-forming-unit fibroblastic activity and can be propagated as non-adherent 'mesenspheres' that can self-renew and expand in serial transplantations. Nestin(+) MSCs are spatially associated with HSCs and adrenergic nerve fibres, and highly express HSC maintenance genes. These genes, and others triggering osteoblastic differentiation, are selectively downregulated during enforced HSC mobilization or beta3 adrenoreceptor activation. Whereas parathormone administration doubles the number of bone marrow nestin(+) cells and favours their osteoblastic differentiation, in vivo nestin(+) cell depletion rapidly reduces HSC ...
  https://www.semanticscholar.org/paper/Mesenchymal-and-haematopoietic-stem-cells-form-a-u-M%C3%A9ndez-Ferrer-Michurina/4c008d7f62d031755b291dbde076d4c86c8e9671
*  Role of PGE2 and other eicosanoids in hematopoietic stem cell function - Louis Pelus
Hematopoietic stem cell transplant is a potentially curative therapy for hematologic and non-hematologic and genetic disorders;however limitations in stem cell...
  http://grantome.com/grant/NIH/R01-HL096305-04
*  CFU-GEMM - Wikipedia
CFU-GEMM is a colony forming unit that generates myeloid cells. CFU-GEMM cells are the multipotential progenitor cells for myeloid cells; they are thus also called common myeloid progenitor cells or myeloid stem cells. "GEMM" stands for granulocyte, erythrocyte, monocyte, megakaryocyte. The common myeloid progenitor (CMP) and the common lymphoid progenitor (CLP) are the first branch of cell differentiation in hematopoiesis after the hemocytoblast (hematopoietic stem cell). In current terminology, CFU-S refers to the pluripotent stem cells that can differentiate into all types of blood cells. CFU-S divides into two lineages: the lymphoid precursor (CFU-LSC) and the myeloid precursor (CFU-GEMM). The CFU-GEMM cell is capable of differentiating into white blood cells, red blood cells, and platelets, all of which are normally found in circulating blood. It has been suggested that eosinophils do not derive from the common myeloid progenitor in ...
  https://en.wikipedia.org/wiki/CFU-GEMM
*  Lymphoid-restricted development from multipotent candidate murine stem cells: distinct and complimentary functions of the c-kit...
The two tyrosine kinase receptors, c-kit and flt3, and their respective ligands KL and FL, have been demonstrated to play key and nonredundant roles in regulating the earliest events in hematopoiesis. However, their precise roles and potential interactions in promoting early lymphoid commitment and development remain unclear. Here we show that most if not all murine Lin(-/lo)Sca1(+)c-kit(+) bone marrow (BM) cells generating B220(+)CD19(+) proB-cells in response to FL and interleukin-7 (IL-7) also have a myeloid potential. In contrast to FL + IL-7, KL + IL-7 could not promote proB-cell formation from Lin(-/lo)Sca1(+)c-kit(+) cells. However, KL potently enhanced FL + IL-7-stimulated proB-cell formation, in part through enhanced recruitment of FL + IL-7-unresponsive Lin(-/lo)Sca1(+)c-kit(+) progenitors, and in part by enhancing the growth of proB-cells. The enhanced recruitment (4-fold) in response to KL occurred exclusively from the Lin(-/lo)Sca1(+)c-kit(+)flt3(-) long-term repopulating stem ...
  https://www.rdm.ox.ac.uk/publications/573551
*  Extended in vitro expansion of adult, mobilized CD34+ cells without significant cell senescence using a stromal cell coculture...
The macrophage colony-stimulating factor-deficient bone marrow stromal cell line OP9, derived from osteopetrotic mice, is known to support hematopoietic stem cell (HSC) expansion as well as hematopoietic differentiation of embryonic stem cells. Coculture of HSC in the OP9 system requires cytokine support to achieve significant cell expansion. Recently, we reported extensive expansion without cell senescence of cord blood (CB)-derived HSC cocultured with OP9 stromal cells for more than 18 weeks with a single cytokine support using human thrombopoietin (TPO). In this study, we evaluated the efficiency of the OP9/TPO coculture system to sustain long-term hematopoiesis of adult, granulocyte colony-stimulating factor mobilized human peripheral blood (PB) CD34(+) cells. Maximum cell expansion was attained during the first 4 weeks of coculture. At the same time, the maximum progenitor cell expansion was ...
  https://www.semanticscholar.org/paper/Extended-in-vitro-expansion-of-adult-mobilized-CD3-Weisel-Moore/183c53319b0509f5b80b576c1af92b24cfaa753b
*  Patent US5491090 - Embryogenic coniferous liquid suspension cultures - Google Patents
This invention relates to a method for regeneration of coniferous plants. In particular, this invention relates to an improved method for establishing and maintaining embryogenic liquid suspension cultures for use in somatic embryogenesis processes from plants of the genus Pinus and Pinus interspecies hybrids. This method is well suited for producing clonal planting stock useful for reforestation.
  http://www.google.com/patents/US5491090?dq=oakley+D523,461&ei=qiI4T-CjGqXf0QHz_PSUCA
*  Retinoic Acid Puts Hematopoietic Stem Cells Back To Sleep
article{6e2d8461-00f2-449a-8017-c9d0475e1207, abstract = {,p,Dormant hematopoietic stem cells (dHSCs) display superior serial reconstitution capacity compared to active HSCs, although their role in normal hematopoiesis has not been thoroughly investigated. Recently in Cell, Cabezas-Wallscheid et al. (2017) demonstrate involvement of retinoic acid signaling in murine dHSCs for preservation of the HSC pool.,/p,}, author = {Rundberg Nilsson, Alexandra and Pronk, Kees-Jan}, issn = {1934-5909}, language = {eng}, month = {07}, number = {1}, pages = {9--11}, publisher = {Cell Press}, series = {Cell Stem Cell}, title = {Retinoic Acid Puts Hematopoietic Stem Cells Back To Sleep}, url = {http://dx.doi.org/10.1016/j.stem.2017.06.002}, volume = {21}, year = {2017 ...
  https://lup.lub.lu.se/search/publication/6e2d8461-00f2-449a-8017-c9d0475e1207
*  British Library EThOS: Transcriptional programmes of haemopoietic stem cells: regulation of the gene encoding the murine stem...
Haemopoietic stem cells are capable of self-replicating and differentiating along eight distinct lineages; these properties allow them to reconstitute the entire blood system of irradiated recipients. How these cells choose whether to self-renew or commit to a specific lineage remains unclear, but transcription factors are likely to play an important role in this decision. To gain insight into the transcriptional programmes of haemopoietic stem cells, the regulation of the gene encoding the murine stem cell antigen Cd34 was analysed. Within haemopoiesis, Cd34 expression is restricted to early progenitor cells, but outside haemopoiesis, Cd34 is expressed in vascular endothelium, fibroblasts, brain and testis. Three key regulatory elements were identified: (a) a3 Kb upstream region which functions as a stem-cell specific, chomatin-dependent enhancer in cell-lines, (b) a6 Kb downstream region which seems to have the ability to ...
  http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.394395
*  Development and aging of primitive hematopoietic stem cells in BALB/c" by J Chen, C M. Astle et al.
Evaluating the function of an individual hematopoietic stem cell (HSC) is a difficult and important problem. The functional ability per HSC, as well as the HSC concentration, was measured with minimal disruption to the cells in vivo using the new competitive dilution assay. Distribution of HSC into recipients was modeled based on Poisson probabilities. Predictions of donor contributions from models assuming different levels of donor HSC functional ability and concentration were compared to actual observations. The model with the least difference between predictions and observations was accepted. In BALB/ cBy (BALB) mice, models assuming equal functional ability of HSC from the same donor fit extremely well with actual observations, suggesting that all HSC are functionally homogeneous at any particular time point during development or aging. Relative HSC functional ability per cell declined during development, so that a fetal HSC had 1.6 to 3.0 times the ...
  https://mouseion.jax.org/stfb1990_1999/1218/