N-methyl-D-aspartate receptors in human erythroid precursor cells and in circulating red blood cells contribute to the...
The presence of N-methyl-d-aspartate receptor (NMDAR) was previously shown in rat red blood cells (RBCs) and in a UT-7/Epo human myeloid cell line differentiating into erythroid lineage. Here we have characterized the subunit composition of the NMDAR and monitored its function during human erythropoiesis and in circulating RBCs. Expression of the NMDARs subunits was assessed in erythroid progenitors during ex vivo erythropoiesis and in circulating human RBCs using quantitative PCR and flow cytometry. Receptor activity was monitored using a radiolabeled antagonist binding assay, live imaging of Ca(2+) uptake, patch clamp, and monitoring of cell volume changes. The receptor tetramers in erythroid precursor cells are composed of the NR1, NR2A, 2C, 2D, NR3A, and 3B subunits of which the glycine-binding NR3A and 3B and glutamate-binding NR2C and 2D subunits prevailed. Functional receptor is required for survival ...http://www.zora.uzh.ch/id/eprint/89788/
Cord Blood CD36+ Early Erythroid Progenitor Cells | Creative Bioarray
Cord Blood-CD36+ Early Erythroid Progenitor cells are derived from Cord Blood-CD34+ cells in culture. Cord Blood-CD34+ cells are cultured for seven to eight days using StemSpan SFEM (StemCell Technologies, Inc.), a serum free expansion media for hematopoietic cells, EPO (3U/ml), SCF (50ng/ml), IL-3 (10ng/ml), and IL-6 (10ng/ml) growth factors. The cells are harvested from culture, and the Cord Blood-CD36+ cells are positively isolated using a direct immunomagnetic CD36 MicroBead labeling system ...https://www.creative-bioarray.com/Cord-Blood-CD36-Early-Erythroid-Progenitor-Cells-CSC-C4527X-item-2247.htm
CFU-E - Wikipedia
CFU-E stands for Colony Forming Unit-Hematopoietic . It arises from CFU-GEMM (via BFU-E, which stands for "erythroid burst-forming units") and gives rise to proerythroblasts. Understanding the murine CFU-e assay (analogous to human assay): CFU-e is a stage of erythroid development between the BFU-e stage and the pro-erythroblast stage. CFU-e colony assay is designed to detect how many colony-forming-units of erythroid lineage there are in a hematopoietic tissue (bone marrow, spleen, or fetal liver), which may be reflective of the organism's demand for oxygen delivery to the tissues or a hematopoietic disorder. Early erythroid progenitors are found at a quite low frequency relative to later stages of erythroid differentiation, such as the pro-erythroblast and the basophilic erythroblast stages which can be detected by flowcytometry directly ex-vivo (Socolovsky et al. 2001 ...https://en.wikipedia.org/wiki/CFU-E
Characterization of the erythropoiesis in myelodysplasia by means of ferrokinetic studies, in vitro erythroid colony formation...
In refractory anemia (RA) and refractory anemia with ringed sideroblasts (RARS) a discrepancy is observed between the decreased in vitro erythroid colony formation and the normal or increased number of normoblasts in the bone marrow. To study the inhttp://www.biomedsearch.com/nih/Characterization-erythropoiesis-in-myelodysplasia-by/9529128.html
Parvovirus B19 Replication and Expression in Differentiating Erythroid Progenitor Cells - pdf descargar
Parvovirus B19 Replication and Expression in Differentiating Erythroid Progenitor Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.http://libros.duhnnae.com/2017/jun9/149853460197-Parvovirus-B19-Replication-and-Expression-in-Differentiating-Erythroid-Progenitor-Cells.php
Erythropoietin-induced erythroid precursor pool depletion causes erythropoietin hyporesponsiveness.
The purpose of this study is to demonstrate that the erythroid precursor depletion in bone marrow induced by recombinant human erythropoietin (rHuEPO) treatment may be another contributing factor to erythropoietin hyporesponsiveness. Healthy Wihttp://www.biomedsearch.com/nih/Erythropoietin-Induced-Erythroid-Precursor-Pool/23187865.html
Modulation of intracellular calcium ([Ca(2+)](i)) by erythropoietin (Epo) is an important signaling pathway controlling erythroid proliferation and differentiation. Transient receptor potential (TRP) channels TRPC3 and homologous TRPC6 are expressed on normal human erythroid precursors, but Epo stimulates an increase in [Ca(2+)](i) through TRPC3 but not TRPC6. Here, the role of specific domains in the different responsiveness of TRPC3 and TRPC6 to erythropoietin was explored. TRPC3 and TRPC6 TRP domains differ in seven amino acids. Substitution of five amino acids (DDKPS) in the TRPC3 TRP domain with those of TRPC6 (EERVN) abolished the Epo-stimulated increase in [Ca(2+)](i). Substitution of EERVN in TRPC6 TRP domain with DDKPS in TRPC3 did not confer Epo responsiveness. However, substitution of TRPC6 TRP with DDKPS from TRPC3 TRP, as well as swapping the TRPC6 distal C terminus (C2) with that of TRPC3, resulted in a chimeric TRPC6 channel with Epo ...http://trpchannel.org/references/21757714
The main finding of this work is that free uptake of the morpholino oligonucleotide ON-654 into the human erythroid cells resulted in nearly 80% of correction, a yield higher than that in syringe-loaded cells. Thus, this oligonucleotide was able to penetrate the erythroid precursor cell membrane barrier and translocate to the nucleus, suggesting that similar result should be possible in vivo. In contrast, our attempts of nuclear delivery of free negatively charged oligonucleotides were unsuccessful.. In cultured patient cells, the time course of repair by free uptake of ON-654 oligonucleotide seems to be very slow. In days 1 to 8, the repair is minimal, increasing in days 8 to 15 and even more so in days 15 to 17 (Fig. 5B). The simplest interpretation of these results is slow uptake of the morpholino oligonucleotide. However, the comparison of free uptake and syringe loading and, in particular, the analysis ...http://molpharm.aspetjournals.org/content/62/3/545
The role of spatial organization of cells in erythropoiesis | SpringerLink
Erythropoiesis, the process of red blood cell production, occurs mainly in the bone marrow. The functional unit of mammalian erythropoiesis, the erythroblastic island, consists of a central macrophage surrounded by adherent erythroid progenitor cells (CFU-E/Pro-EBs) and their differentiating progeny, the erythroblasts. Central macrophages display on their surface or secrete various growth or inhibitory factors that influence the fate of the surrounding erythroid cells. CFU-E/Pro-EBs have three possible fates: (a) expansion of their numbers without differentiation, (b) differentiation into reticulocytes that are released into the blood, (c) death by apoptosis. CFU-E/Pro-EB fate is under the control of a complex molecular network, that is highly dependent upon environmental conditions in the erythroblastic island. In order to assess the functional role of space coupled with the complex network behavior in erythroblastic islands, we developed ...https://link.springer.com/article/10.1007%2Fs00285-014-0758-y
Regulated expression of multiple chicken erythroid membrane skeletal protein 4.1 variants is governed by differential RNA...
Protein 4.1 is an extrinsic membrane protein that facilitates the interaction of spectrin and actin in the erythroid membrane skeleton and exists as several structurally related polypeptides in chickens. The ratio of protein 4.1 variants is developmentally regulated during terminal differentiation of chicken erythroid and lenticular cells. To examine the mechanisms by which multiple chicken protein 4.1 variants are differentially expressed, we have isolated cDNA clones specific for chicken erythroid protein 4.1. We show that a single protein 4.1 gene gives rise to multiple 6.6-kilobase mRNAs by differential RNA processing. Furthermore, the ratios of protein 4.1 mRNAs change during chicken embryonic erythropoiesis. We observe a quantitative difference in variant ratios when protein 4.1 is synthesized in vivo or in a rabbit reticulocyte lysate in vitro. Our results show that the expression of multiple protein 4.1 polypeptides is regulated at ...http://authors.library.caltech.edu/10646/
Unexpected role for p19INK4d in post-transcriptional regulation of GATA1 and modulation of human terminal erythropoiesis |...
Terminal erythroid differentiation is tightly coordinated with cell cycle exit, which is regulated by cyclins, cyclin-dependent kinases and cyclin-dependent kinase inhibitors (CDKI), yet their roles in erythropoiesis remain to be fully defined. We show here that p19INK4d, a member of CDKI family, is abundantly expressed in erythroblasts and that p19INK4d knockdown delayed erythroid differentiation, inhibited cell growth, led to increased apoptosis and generation of abnormally nucleated late stage erythroblasts. Unexpectedly, p19INK4d knockdown did not affect cell cycle. Rather it led to decreased expression of GATA1 protein. Importantly, the differentiation and nuclear defects were rescued by ectopic expression of GATA1. As GATA1 protein is protected by nuclear HSP70, we examined the effects of p19INK4d knockdown on HSP70 and found that p19INK4d knockdown led to decreased expression of HSP70 and its nuclear localization. The reduced levels of HSP70 are the result of reduced ...http://www.bloodjournal.org/content/early/2016/11/22/blood-2016-09-739268?sso-checked=true
In Vitro Large Scale Production of Human Mature Red Blood Cells from Hematopoietic Stem Cells by Coculturing with Human Fetal...
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject-specific sections.https://www.hindawi.com/journals/bmri/2013/807863/cta/
Inactivation of erythropoietin leads to defects in cardiac morphogenesis | Development
Erythropoietin is an essential growth factor that promotes survival, proliferation, and differentiation of mammalian erythroid progenitor cells. Erythropoietin(−/−) and erythropoietin receptor(−/−) mouse embryos die around embryonic day 13.5 due, in part, to failure of erythropoiesis in the fetal liver. In this study, we demonstrated a novel role of erythropoietin and erythropoietin receptor in cardiac development in vivo. We found that erythropoietin receptor is expressed in the developing murine heart in a temporal and cell type-specific manner: it is initially detected by embryonic day 10.5 and persists until day 14.5. Both erythropoietin(−/−) and erythropoietin receptor(−/−) embryos suffered from ventricular hypoplasia at day 12-13 of gestation. This defect appears to be independent from the general state of hypoxia and is likely due to a reduction in the number of proliferating cardiac myocytes in the ventricular myocardium. Cell proliferation assays revealed that ...http://dev.biologists.org/content/126/16/3597
Saved from Proteolysis | Science Signaling
Terminal erythroid differentiation in response to erythropoietin depends on caspase-3 activation, whereas erythropoietin withdrawal leads to caspase-mediated proteolysis of the transcription factor GATA-1 and apoptosis. Ribeil et al. observed that activated caspase-3 colocalized in the nucleus with GATA-1 during erythroid differentiation and wondered how GATA-1 escaped proteolysis. They found that during differentiation, the molecular chaperone Hsp3 (which was expressed constitutively by differentiating human erythroblasts) colocalized in the nucleus with GATA-1, whereas Hsp70 disappeared from the nucleus during erythropoietin starvation. Hsp70 coimmunoprecipitated with GATA-1 from differentiated erythroblasts and protected GATA-1 from caspase-3-mediated cleavage in an in vitro proteolysis assay. Hsp70 knockdown with siRNA led to GATA-1 degradation in differentiating cells as well as to a decrease in the proportion of differentiating cells ...http://stke.sciencemag.org/content/2007/368/tw11
Developmental regulation of myeloerythroid progenitor function by the Lin28b-let-7-Hmga2 axis | JEM
Hematopoiesis is a tightly regulated but adaptable process in which production of terminally differentiated cells responds to pathophysiologic stimuli such as hypoxia, hemorrhage, infection, or inflammation (Orkin and Zon, 2008; Paulson et al., 2011; Takizawa et al., 2012). Basal myeloerythroid output is poised to meet specific developmental stage-specific demands, with erythroid production predominant in the FL to support growth under hypoxic conditions and granulocyte production favored in the adult BM to provide innate immunity and tissue repair capability. Candidate regulators of hematopoietic developmental maturation have begun to emerge (He et al., 2011; Mochizuki-Kashio et al., 2011; Xie et al., 2014). In this study, we document developmental maturation of myeloerythroid progenitors and link this mechanistically to regulation by the Lin28-let-7-Hmga2 axis, which is known to control developmental timing from worms to ...http://jem.rupress.org/content/213/8/1497
Developmental regulation of myeloerythroid progenitor function by the Lin28b...
Hematopoiesis is a tightly regulated but adaptable process in which production of terminally differentiated cells responds to pathophysiologic stimuli such as hypoxia, hemorrhage, infection, or inflammation (Orkin and Zon, 2008; Paulson et al., 2011; Takizawa et al., 2012). Basal myeloerythroid output is poised to meet specific developmental stage-specific demands, with erythroid production predominant in the FL to support growth under hypoxic conditions and granulocyte production favored in the adult BM to provide innate immunity and tissue repair capability. Candidate regulators of hematopoietic developmental maturation have begun to emerge (He et al., 2011; Mochizuki-Kashio et al., 2011; Xie et al., 2014). In this study, we document developmental maturation of myeloerythroid progenitors and link this mechanistically to regulation by the Lin28-let-7-Hmga2 axis, which is known to control developmental timing from worms to ...http://jem.rupress.org/content/early/2016/07/05/jem.20151912
The majority of the in vitro erythroid expansion potential resides in CD34− cells, outweighing the contribution of CD34+ cells...
During the first days, the expanding and differentiating CD34− progenitors progress to become CD34+ cells (Online Supplementary Figures S1 and S2), and the percentage of this CD34+ population increases with time (Online Supplementary Figure S1). At day 3 of culture, the CD34+ cells are c-kit+ and CD71low/medium but GPA− which may indicate a mixture of common myeloid and megakaryocyte/erythroid common progenitors (Online Supplementary Figure S2). Interestingly, the CD34− PBMCs were largely devoid of erythroid colony forming ability upon isolation from the peripheral blood. This has been described before in a subset of CD34− hematopoietic stem cells, the side population cells or SP cells.13 However, a gradual increase of erythroid colony formation was observed using our in vitro culture conditions, coinciding with the appearance of CD34+ cells ...http://www.haematologica.org/content/95/9/1594
anemia, dyserythropoietic, congenital - CISMeF
A familial disorder characterized by ANEMIA with multinuclear ERYTHROBLASTS, karyorrhexis, asynchrony of nuclear and cytoplasmic maturation, and various nuclear abnormalities of bone marrow erythrocyte precursors (ERYTHROID PRECURSOR CELLS). Type II is the most common of the 3 types; it is often referred to as HEMPAS, based on the Hereditary Erythroblast Multinuclearity with Positive Acidified Serum test. - anemia, dyserythropoietic, congenital -http://www.chu-rouen.fr/page/DOC_127121
Developmental Biology of Human Erythropoiesis - Orkin Stuart
In this competitive renewal application of this Program Project grant now in its 30th year, multicjisciplinary approaches are employed to address the central th...http://grantome.com/grant/NIH/P01-HL032262-33
JoVE Author Search: Begley CG
Erythropoietin, Erythropoiesis, Antibodies, Cell, Cells, Erythroid Progenitor Cells, Human, Progenitor Cells, Erythrocytes, Erythroid Cells, Flow Cytometry, Gene, Immunoblotting, Metabolism, Methods, Monoclonal Antibodies, Proteins, Role, Specificity, Understandinghttp://labindex.jove.com/author/Begley-CG
Red Blood Cells Can Now Be Mass-Produced
Many health conditions require blood transfusion. And though many people donate blood, the supply always seems insufficient. Which is why science had to step up through lab-made red blood cells.. As it is, the focus of research in this field is on stem cell donation, and growing these stem cells directly into mature red blood cells. However, this method presents two basic problems. First, it only produces a limited number of mature cells. Second, repeat donation is necessary because the donated stem cells eventually burn out and die.. The method developed by a team of researchers at the University of Bristol and NHS Blood and Transplant addresses both these problems.. The technique involves trapping of stem cells at an early age, in other words, premature red cells. These can then be cultured indefinitely. 'Immortalizing' is the term for it. And once these 'immortal' cells ...http://wallstreetpit.com/113162-red-blood-cells-mass-produced/
Do red blood cells have nuclei? | Reference.com
Mature red blood cells do not contain nuclei. However, red blood cells that are not fully formed and not fully matured do contain nuclei for a brief period of time during their development....https://www.reference.com/science/red-blood-cells-nuclei-688b4c86cb2cd4f0
Biology-Online • View topic - Cancer
Good question. My first reaction was that every cell can get cancer. I say this because every cell has the whole DNA genome in it - except for mature red blood cells cause they eject their nucleus by a type of cell division process. Mmm..... maybe them, as most cancers have to have a genomic DNA with mutations to have a functional disfunction (does that make sense?) to be called cancer. The only other one that I was going to say was perhaps a germ cell. They are just a quiet type of cell that is quiescent, so they really do not function till called upon to do so by factors in the environment. The only time it could become cancerous is when it starts functioning and is mitotically active. How about the other quiescent cells in the body? The satellite stem cells perhaps? Though once they re-enter the cell cycle, they can then have the capacity for becoming mutated and cancerous, so then it is just a matter of time ...http://www.biology-online.org/biology-forum/post-116900.html
MicroRNA expression dynamics during murine and human erythroid differentiation. | Sigma-Aldrich
Sigma-Aldrich offers abstracts and full-text articles by [Mei Zhan, Chris P Miller, Thalia Papayannopoulou, George Stamatoyannopoulos, Chao-Zhong Song].http://www.sigmaaldrich.com/catalog/papers/17588470
Erythroblasts synonyms, Erythroblasts antonyms - FreeThesaurus.com
Synonyms for Erythroblasts in Free Thesaurus. Antonyms for Erythroblasts. 3 words related to erythroblast: embryonic cell, formative cell, sideroblast. What are synonyms for Erythroblasts?http://www.freethesaurus.com/Erythroblasts