*  Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha | The EMBO Journal
Ketting RF, Haverkamp TH, vanLuenen HG, Plasterk RHA (2001) Dicer functions in RNA interference and in synthesis of small RNA ... miRNA hairpins are highly variable in sequence and, moreover, are predicted to fold into RNA hairpins that, while similar in ... Hutvágner G, McLachlan J, Pasquinelli AE, Balint E, Tuschl T, Zamore PD (2001) A cellular function for the RNAinterference ... Yi R, Qin Y, Macara IG, Cullen BR (2003) Exportin‐5 mediates the nuclear export of pre‐microRNAs and short hairpin RNAs. Genes ...
  http://emboj.embopress.org/content/24/1/138
*  DNA-directed RNA interference - Wikipedia
DNA constructs are designed to express self-complementary double-stranded RNAs, typically short-hairpin RNAs (shRNA), that once ... Any RNA, including endogenous mRNAs or viral RNAs, can be silenced by designing constructs to express double-stranded RNA ... DNA-directed RNA interference (ddRNAi) is a gene-silencing technique that utilizes DNA constructs to activate an animal cell's ... Lambeth, L. S.; Smith, C. A. (2013). "Short Hairpin RNA-Mediated Gene Silencing". SiRNA Design. Methods in Molecular Biology. ...
  https://en.wikipedia.org/wiki/DNA-directed_RNA_interference
*  Wnt4 overexpression promotes thymoma development through a JNK‑mediated planar cell polarity‑like pathway
Wnt4 gene expression was then blocked in thymoma cells by short hairpin (sh)RNA interference, and changes in JNK mRNA and ... shRNA, short hairpin RNA; Lane M, marker; lane 1, control; lane 2, TR001; lane 3, shRNA-Wnt4-1; lane 4, shRNA-Wnt4-2; lane 5, ... HSH13446-2-CH2 interference plasmid; Wnt4-shRNA-3, HSH13446-3-CH3 interference plasmid; and Wnt4-shRNA-4, HSH13446-4-CH4 ... Total RNA (1 µg) was reverse transcribed into cDNA and quantified by RT-qPCR using SYBR Premix Ex Taq™ (Takara Bio, Inc., Otsu ...
  https://www.spandidos-publications.com/ol/15/1/83
*  CiNii 論文 - Introduction of silencing-inducing transgene against Fgf19 does not affect...
RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells YU JY ... Plasmid-based short-hairpin RNA interference in the chicken embryo CHESNUTT C. ... Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells PADDISON PJ ... Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference UI-TEI K. ...
  https://ci.nii.ac.jp/naid/10021095808
*  Pharmaceutically controlled designer circuit for the treatment of the metabolic syndrome | PNAS
2006) Short hairpin RNA-expressing bacteria elicit RNA interference in mammals. Nat Biotechnol 24(6):697-702. ... RNA Isolation and RT-PCR.. Total RNA was extracted from mammalian cells using the RNeasy kit (Qiagen) and RT-PCR was performed ... 2010) Reprogramming cellular behavior with RNA controllers responsive to endogenous proteins. Science 330(6008):1251-1255. ...
  http://www.pnas.org/content/110/1/141?ijkey=2f60a7e1ee18be25edbaffff62e473d771d332de&keytype2=tf_ipsecsha
*  Protocols and Video Articles Authored by Robert C. Robbins (Translated to Russian)
Short Hairpin RNA Interference Therapy for Ischemic Heart Disease Circulation. Sep, 2008 , Pubmed ID: 18824759 During hypoxia, ... Here we hypothesize that short hairpin RNA (shRNA) interference therapy targeting PHD2 can be used for treatment of myocardial ... Using HLA I RNA interference and intrabody technology, HLA I surface expression of hESC(KD) was 88%-99% reduced. T cell ...
  https://www.jove.com/author/Robert+C._Robbins?language=Russian
*  Benefits and Risks of Manipulating the HIF Hydroxylase Pathway in Ischemic Heart Disease | Springer for Research & Development
Short hairpin RNA interference therapy for ischemic heart disease. Circulation. 2008;118:S226-33.PubMedCrossRefGoogle Scholar ...
  https://rd.springer.com/chapter/10.1007/978-0-85729-920-8_2
*  Derrick Wan | Stanford Medicine Profiles
Abstract 151: short hairpin RNA interference therapy for diabetic murine wound closure and hindlimb ischemia. Plastic and ... Short Hairpin RNA Silencing of PHD-2 Improves Neovascularization and Functional Outcomes in Diabetic Wounds and Ischemic Limbs. ... Here we investigated the therapeutic potential of inhibiting HIF-1α degradation through short hairpin RNA silencing of PHD-2 in ... and RNA was harvested at days 0, 1, 3, 5, and 7. Directional RNA sequencing libraries were prepared and sequenced. Paired-end ...
  https://med.stanford.edu/profiles/derrick-wan
*  Highly Improved Gene Targeting by Germline-Specific Cas9 Expression in Drosophila | Genetics
2005 Drosophila U6 promoter-driven short hairpin RNAs effectively induce RNA interference in Schneider 2 cells. Biochem. ... We and others have successfully generated genome-wide collections of transgenic RNA interference lines and distributed them to ... Although it takes an additional time of at least 1 month compared to direct injection of DNA or RNA encoding Cas9 and gRNA ... 2013 Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Genetics 194: 1029-1035. ...
  http://www.genetics.org/content/195/3/715.full
*  Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease | Genetics
2005 Drosophila U6 promoter-driven short hairpin RNAs effectively induce RNA interference in Schneider 2 cells. Biochem. ... 2006 A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic ... a CRISPR RNA (crRNA), which contains sequence complementary to invading virus or plasmid DNA, and a trans-activating CRISPR RNA ... This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila. ...
  http://www.genetics.org/content/194/4/1029.long
*  Share link to this page
CAPN5 gene silencing by short hairpin RNA interference.. Nelson NG, Skeie JM, Muradov H, Rowell HA, Seo S, Mahajan VB. BMC Res ... RNA interference-based therapy for spinocerebellar ataxia type 7 retinal degeneration.. Ramachandran PS, Bhattarai S, Singh P, ...
  http://www.rpbusa.org/rpb/grants-and-research/research/db/?institution=132206&granttype=55486&start=%2B&start_other=2014
*  A small cassette enables conditional gene inactivation by CRISPR/Cas9, Scientific Reports | 10.1038/s41598-017-16931-z |...
Specific knockdown of OCT4 in human embryonic stem cells by inducible short hairpin RNA interference ... Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression ...
  https://www.deepdyve.com/lp/springer_journal/a-small-cassette-enables-conditional-gene-inactivation-by-crispr-cas9-d2uGbFgSAY
*  Cited In for PubMed (Select 14730625) - PubMed - NCBI
Lentivirus-mediated short hairpin RNA interference targeting TNF-alpha in macrophages inhibits particle-induced inflammation ...
  https://www.ncbi.nlm.nih.gov/pubmed?linkname=pubmed_pubmed_citedin&from_uid=14730625
*  Connexin 36 Expression Regulates Neuronal Differentiation from Neural Progenitor Cells
Harper SQ, Davidson BL (2005) Plasmid-based RNA interference: construction of small-hairpin RNA expression vectors. Methods in ... 219-235.SQ HarperBL Davidson2005Plasmid-based RNA interference: construction of small-hairpin RNA expression vectors.Methods in ... Isolated RNA was DNaseI treated (Roche) and the preparation cleaned up using the RNeasy minelute kit (Qiagen). RNA quality and ... At each passage samples were collected for RNA analysis by snap freezing in liquid nitrogen and storing at −80 °C and for ...
  http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0014746
*  Browse by Academic Unit (A-Z) - White Rose Research Online
2 more authors) (2009) Specific Knockdown of OCT4 in Human Embryonic Stem Cells by Inducible Short Hairpin RNA Interference. ... 1 more author) (2010) A direct comparison of strategies for combinatorial RNA interference. BMC Molecular Biology, 11. 77. ISSN ... 3 more authors) (2010) RNA microarray analysis in prenatal mouse cochlea reveals novel IGF-I target genes: implication of MEF2 ... 3 more authors) (2010) RNA microarray analysis in prenatal mouse cochlea reveals novel IGF-I target genes: implication of MEF2 ...
  http://eprints.whiterose.ac.uk/view/iau/Sheffield=2EBMS.html
*  Short hairpin RNA - Wikipedia
Xiang, Shuanglin; Fruehauf, Johannes; Li, Chiang J (2006). "Short hairpin RNA-expressing bacteria elicit RNA interference in ... A short hairpin RNA or small hairpin RNA (shRNA/Hairpin Vector) is an artificial RNA molecule with a tight hairpin turn that ... Small interfering RNA Paddison, PJ; Caudy, AA; Bernstein, E; Hannon, GJ; Conklin, DS (15 April 2002). "Short hairpin RNAs ( ... Wang, Zhaohui; Rao, Donald D.; Senzer, Neil; Nemunaitis, John (2011). "RNA Interference and Cancer Therapy". Pharmaceutical ...
  https://en.wikipedia.org/wiki/Short_hairpin_RNA
*  miR-shRNA expression options. A) Flow chart for express | Open-i
A versatile approach to multiple gene RNA interference using microRNA-based short hairpin RNAs. ... A versatile approach to multiple gene RNA interference using microRNA-based short hairpin RNAs. ... We have developed a flexible platform using RNA polymerase II promoter-driven expression of microRNA-like short hairpin RNAs ... We have developed a flexible platform using RNA polymerase II promoter-driven expression of microRNA-like short hairpin RNAs ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC2194719_1471-2199-8-98-5&req=4
*  Plus it
Lentiviral vectors carrying short hairpin RNA interference (RNAi) targeting human PKCι were generated and used to obtain stable ... Yasuhiro Ikeda of the Mayo Clinic (Rochester, MN) for providing the pSIN-Fluc vector; the Mayo Clinic RNA Interference ... Data were normalized to 18S RNA abundance (×104) to control for RNA concentration. Horizontal line, 2 SD above the mean PKCι ... RNA isolation, quantitative real-time PCR, and analysis. H&E-stained sections of matched normal and pancreatic tumor tissues ...
  http://cancerres.aacrjournals.org/content/70/5/2064
*  Plus it
Oligodeoxynucleotides coding for small hairpin RNA interference (shRNAi) directed against murine ADAM-10 (small interfering RNA ... RNA isolation, reverse transcription, design of primer and probes, and quantitative RT-PCR. RNA isolation and reverse ... lacZ mRNA and 18S RNA levels were detected in total RNA of HT1080lacZ-K15 metastasis-bearing livers by quantitative RT-PCR. Top ... Top right, columns, mean of relative lacZ mRNA versus 18S RNA; bars, SE. Addl70-3: 0.29 ± 0.16, n = 4; AdTIMP-1: 2.25 ± 0.62, n ...
  http://cancerres.aacrjournals.org/content/67/18/8615
*  Co-activator SRC-1 is dispensable for transcriptional control by STAT3 | Biochemical Journal
RNA interference; shRNA, small hairpin RNA; SOCS3, suppressor of cytokine signalling 3; SRC, steroid receptor co-activator; ... In fact, silencing of SRC-1 by RNA interference rapidly induced apoptosis in IL-6-dependent INA-6 human myeloma cells, ...
  http://www.biochemj.org/content/420/1/123
*  Addgene: pLVUT-tTR-KRAB
Addition of Pol III promoter-small hairpin RNA cassette allows for drug-controllable RNA interference (Tet-on shRNA). ... Transgenes can be expressed from any RNA Pol II promoter as part of bicistronic unit comprising the KRAB-based repressor; tetO ...
  https://www.addgene.org/11651/
*  TRPM7 regulates polarized cell movements | Biochemical Journal
RNA interference; shRNA, short hairpin RNA; SLC41A2, solute carrier family 41 member 2; TRPM7, transient receptor potential ... In the present study we show that depletion of TRPM7 by RNA interference in fibroblasts alters cell morphology, the ...
  http://www.biochemj.org/content/434/3/513
*  XPO5 - Wikipedia
"Overexpression of exportin 5 enhances RNA interference mediated by short hairpin RNAs and microRNAs". RNA. 11 (2): 220-6. doi: ... Wilson RC, Doudna JA (2013). "Molecular mechanisms of RNA interference". Annual Review of Biophysics. 42: 217-39. doi:10.1146/ ... "Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs". Genes & Development. 17 (24): 3011-6. doi: ... RNA. 10 (2): 185-91. doi:10.1261/rna.5167604. PMC 1370530 . PMID 14730017. Chen T, Brownawell AM, Macara IG (Aug 2004). " ...
  https://en.wikipedia.org/wiki/XPO5
*  Novel and Stress-Regulated MicroRNAs and Other Small RNAs from Arabidopsis | Plant Cell
Elbashir, S.M., Lendeckel, W., and Tuschl, T. (2001). RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev. 15 ... The ability of RNA corresponding to the genomic sequences surrounding the clones, to fold into hairpin miRNA precursors, was ... Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans ... RNA Gel Blot Analysis. Total RNA was isolated from 2-week-old seedlings (Columbia ecotype) grown on MS nutrient-agar plates ( ...
  http://www.plantcell.org/content/16/8/2001.full