*  Protein scaffold-based molecular probes for cancer molecular imaging
Single domain immunoglobin is a class of protein scaffold present in the human single domain antibody (sdAb) and camelidae ... 1997). High affinity nanobodies have also made directly from phage display with VHH gene repertoires without immunizing a ... The protein scaffold-based probes have great potential in diagnosis of disease, in monitoring therapeutic efficacy of new drugs ... post-transcriptional modification in yeast could be a disadvantage and maybe some times an advantage such as glycosylation. ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2914822/?lang=en-ca
*  Discovery Biology News
... with expertise to develop high affinity single domain antibodies for therapeutic use. ... Discovery of these antibodies began in mice, where a key finding involving the glycosylation state of the antibody led to ... With our one-stop services, we are determined to be your best partner in antibody drug discovery from gene synthesis to in-vivo ... or as a new bi-specific antibody therapeutic.. Camelid single domain antibodies are a novel bi-specific antibody platform that ...
  https://www.genscript.com/DiscoveryBiology_news.html
*  Protocols and Video Articles Authored by Victor A. Streltsov
... to create a diverse library of single domain antibody fragments based on the shark IgNAR antibody isotype. Coupling of these ... and a mutant thereof with reduced glycosylation, potentially suitable for preclinical therapeutic and structural studies, ... From a pool of monoclonal antibodies, we selected one such candidate (A4D11) with high affinity for tBclA, a truncated version ... we describe the humanization of WO-2 using complementary determining region loop grafting onto the human germline gene and the ...
  https://www.jove.com/author/Victor+A._Streltsov
*  Engineered Anti-IL-23p19 Antibodies - Patent application
... present invention provides single domain antibodies comprising two VH domains with modifications such that single domain ... adding one or more glycosylation sites that are not present in the antibody. Glycosylation of antibodies is typically either N- ... 0006] Therapeutic antibodies may be used to block cytokine activity. The most significant limitation in using antibodies as a ... single immortalized cells are screened for production of antibodies of the desired specificity and affinity for the antigen, ...
  http://www.patentsencyclopedia.com/app/20110142853
*  Antibody - Wikipedia
Magnetic immunoassay Microantibody Monoclonal antibody Neutralizing antibody Secondary antibodies Single-domain antibody Slope ... diagnostic and therapeutic agents. Affimer Antibody mimetic Anti-mitochondrial antibodies Anti-nuclear antibodies Aptamer ... The chromosomal region that encodes an antibody is large and contains several distinct gene loci for each domain of the ... This serves to increase the diversity of the antibody pool and impacts the antibody's antigen-binding affinity. Some point ...
  https://en.wikipedia.org/wiki/Antibody
*  ANTI-GITR ANTIBODIES - Patent application
... present invention provides single domain antibodies comprising two VH domains with modifications such that single domain ... adding one or more glycosylation sites that are not present in the antibody. Glycosylation of antibodies is typically either N- ... Changes to the Fc can also alter the half-life of antibodies in therapeutic antibodies, and a longer half-life would result in ... production of antibodies of the desired specificity and affinity for the antigen, and yield of the monoclonal antibodies ...
  http://www.patentsencyclopedia.com/app/20120189639
*  ǰ?????? | Antibody Engineering & Therapeutics 2017 ٷ ??վ
Single domain antibodies have a natural tendency to bind cavities and are often sensitive to conformational changes of their ... Examples will include work on antibody fragment affinity improvement by 100-fold to low pM affinity. Also the engineering of ... by CRISPR/Cas9 and TALE nucleases we have constructed large libraries in mammalian cells containing a single antibody gene/cell ... of the IgG Fc have allowed for serum persistence and immune engagement to become tunable properties for therapeutic mabs and Fc ...
  http://cn.giievent.tw/ibu428094/agenda1.shtml
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
However, as another opportunity, these interesting and stable single-domain antibodies8 can be easily engineered with different ... Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis ... the antibody Fc domain does not have constant function, and is strongly modulated by IgG subclass (IgG1-4) and glycosylation at ... There is an urgent need to develop approaches for repairing the damaged heart, discovering new therapeutic drugs that do not ...
  https://www.jove.com/visualize/abstract/24751715/development-vhh-antibodies-against-dengue-virus-type-2-ns1-comparison
*  Patent US6451983 - Tumor necrosis factor antibodies - Google Patents
The ligand is preferably an antibody, F(ab) fragment, single domain antibody (dABs) single chain antibody or a serum binding ... It is preferred, however, that the ligand is a monoclonal antibody or F(ab) fragment thereof. ... Affinity of Binding of the Selected V Gene Pairs:. MAb32 and purified, monomeric forms of the recombinant mouse scFv-MAb32 and ... Assignment of function to such regions would permit the development of MAbs and other ligands of therapeutic use. Polyclonal ...
  http://www.google.co.uk/patents/US6451983
*  Antibody - Wikipedia
The chromosomal region that encodes an antibody is large and contains several distinct gene loci for each domain of the ... Affinity designationsEdit. A group of antibodies can be called monovalent (or specific) if they have affinity for the same ... To obtain antibody that is specific for a single epitope of an antigen, antibody-secreting lymphocytes are isolated from the ... which contains a conserved glycosylation site involved in these interactions.[4] The production of antibodies is the main ...
  https://en.m.wikipedia.org/wiki/Antibody
*  A Human Single-Domain Antibody Elicits Potent Antitumor Activity by Targeting an Epitope in Mesothelin Close to the Cancer Cell...
SD1 is the first human single-domain antibody targeting mesothelin-expressing tumors, shows potential as a cancer therapeutic ... A novel human monoclonal antibody that binds with high affinity to mesothelin-expressing cells and kills them by antibody- ... N-glycosylation as novel strategy to improve pharmacokinetic properties of bispecific single-chain diabodies. J Biol Chem 2008; ... gene encodes approximately 71 kDa precursor protein that is processed to approximately 31 kDa N-terminal protein and ...
  http://mct.aacrjournals.org/content/12/4/416?rss=1
*  The Aggregation of Huntingtin and α-Synuclein.
On the other hand, it was found that engineered single-domain intracellular antibody against Htt inhibits aggregation at low ... Therapeutic Research in α-Synuclein Aggregation As mentioned before, α-syn aggregation has become an important target in PD- ... This section of the text is focused on the protein product of the PARK1 gene, the α-syn protein, due to its unfolded nature and ... Formation of a high affinity lipid-binding intermediate during the early aggregation phase of α-synucleinBiochemistryYear: ...
  http://www.biomedsearch.com/nih/Aggregation-Huntingtin-Synuclein/22899913.html
*  Patent US5182107 - Transferrin receptor specific antibody-neuropharmaceutical or diagnostic ... - Google Patents
Other aspects of this invention include a delivery system comprising an antibody reactive with a transferrin receptor linked to ... The method comprises administering to the host a therapeutically effective amount of an antibody-neuropharmaceutical or ... diagnostic agent conjugate wherein the antibody is reactive with a transferrin receptor. ... O-linked glycosylation of peptides. US8383107. Mar 21, 2011. Feb 26, 2013. National Research Council Of Canada. Single domain ...
  http://www.google.com/patents/US5182107?dq=5,381,459
*  Trends in recombinant protein use in animal production | Microbial Cell Factories | Full Text
produced recombinant llama single-domain antibody fragments (VHHs) using recombinant strains of S. cerevisiae to confer rapid ... To improve the affinity of monovalent scFv, dimeric single-chain mini-antibody molecules, named minibodies or SIPs (small ... Mao D, Bai W, Hui F, Yang L, Cao S, Xu Y. Effect of inhibin gene immunization on antibody production and reproductive ... type 1 neutralization by multimerized single-chain variable antibody fragments regardless of differential glycosylation. Clin ...
  https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-017-0654-4
*  State of play and clinical prospects of antibody gene transfer | Journal of Translational Medicine | Full Text
Therapeutic proof of concept has been demonstrated in mice and non-human primates, and intramuscular vectored mAb therapy is ... RNA presents another emerging platform for antibody gene transfer. Early reports in mice show that mRNA may be able to rival ... To address these issues, antibody gene transfer seeks to administer to patients the mAb-encoding nucleotide sequence, rather ... The current review outlines the state of play and clinical prospects of antibody gene transfer, thereby highlighting recent ...
  https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-017-1234-4
*  SMART: Secondary literature for RICIN domain
To test that hypothesis, single binding domain 2 (SBD2) of ricin B chain was expressed as a gene 3 fusion protein on the ... Both hybrid hormones bound to the anti-porcine relaxin antibody R6 with high affinity, and the insulin analog, with an ... Recombinant B-chain alone was nontoxic to cells (ID50 > 10(-6)M). Our data suggest that N-glycosylation of ricin B-chain is not ... The plant protein toxin ricin has found widespread application as a potential therapeutic agent for many human diseases and in ...
  http://smart.embl-heidelberg.de/smart/show_secondary.cgi?domain=RICIN
*  Antibodies That Bind OV064 and Methods of Use Therefor - Patent application
... which consists of a VH domain; (vii) a single domain functional heavy chain antibody, which consists of a VHH domain (known as ... 0072]The term "monospecific antibody" refers to an antibody that displays a single binding specificity and affinity for a ... Site directed mutagenesis of the antibody CH2 domain to eliminate glycosylation may be preferred in order to prevent changes in ... Most therapeutic antibodies are human, chimeric, or humanized antibodies of the IgG1 type. [0100]The variable regions of each ...
  http://www.patentsencyclopedia.com/app/20090208489
*  Frontiers | Fc Engineering for Developing Therapeutic Bispecific Antibodies and Novel Scaffolds | Immunology
... and dual-affinity-retargeting [DART] are showing exciting results in terms of recruiting and activating self-immune effector ... Promisingly, Fc antigen binding fragment [Fcab] and monomeric antibody or half antibody may be particularly advantageous to ... Promisingly, Fc antigen binding fragment [Fcab] and monomeric antibody or half antibody may be particularly advantageous to ... to antibody fragments. Impressively, antibody fragments such as bispecific T-cell engager [BiTE], bispecific killer cell ...
  https://www.frontiersin.org/articles/10.3389/fimmu.2017.00038/full
*  COMPOSITIONS AND METHODS OF USE FOR THERAPEUTIC ANTIBODIES - Patent application
... antibody lacks glycosylation). Glycosylation can be altered to, for example, increase the affinity of the antibody for the ... the human antibodies of the invention into nanobody or single domain antibody framework sequences, as described for example in ... The antibody light chain gene and the antibody heavy chain gene can be inserted into separate vector or, more typically, both ... The antibody can be, for example, a human antibody, a humanized antibody or a chimeric antibody. Preferably the antibody is a ...
  http://www.patentsencyclopedia.com/app/20120034231
*  ACTRIIB BINDING AGENTS AND USES THEREOF - Patent application
... can be incorporated into single-domain antibodies, single-chain antibodies, maxibodies, minibodies, intrabodies, diabodies, ... the anti-ActRIIB antibody is a therapeutic antibody or functional fragment thereof. An anti-ActRIIB antibody or functional ... 0085] Affinity may be determined by an affinity ELISA assay. In certain embodiments, affinity may be determined by a BIACORE® ... the coated antibody and Ab-17G05 as the solution-phase antibody. [0111] A reporter gene assay in A204 cells may be used to ...
  http://www.patentsencyclopedia.com/app/20130189269
*  ANTIBODY MOLECULES HAVING SPECIFICITY FOR HUMAN IL-6 - Patent application
... single domain antibodies, scFv, bi, tri or tetra-valent antibodies, Bis-scFv, diabodies, triabodies, tetrabodies and epitope- ... Alternatively, IgG2 and TgG4 isotypes may be used when the antibody molecule is intended for therapeutic purposes and antibody ... well and retained the affinity of the donor antibody. This heavy chain graft, comprising only a single donor framework residue ... 0135] It is also envisaged that the antibody of the present invention may be administered by use of gene therapy. In order to ...
  http://www.patentsencyclopedia.com/app/20120183996
*  Anti-ErbB3 Antibodies and Uses Thereof - Patent application
... domain-specific antibodies, single domain antibodies, domain-deleted antibodies, chimeric antibodies, CDR-grafted antibodies, ... Therapeutic Uses of the Antibodies [0086] The antibodies of the invention are useful, inter alia, for the treatment, prevention ... bioequivalent antibodies may include anti-ErbB3 antibody variants comprising amino acid changes which modify the glycosylation ... 0105] As shown in Tables 2-4, many of the exemplary antibodies tested in this Example exhibited high affinity binding to ErbB3 ...
  http://www.patentsencyclopedia.com/app/20160144029
*  FTY720 Increases CD74 Expression and Sensitizes Cancer Cells to Anti-CD74 Antibody-Mediated Cell Death - Patent application
0072] Another form of an antibody fragment is a single-domain antibody (dAb), sometimes referred to as a single chain antibody ... The siRNA, interference RNA or therapeutic gene may be attached to a carrier moiety that is conjugated to an antibody or ... antibody. As simply transferring mouse CDRs into human FRs often results in a reduction or even loss of antibody affinity, ... more glycosylation sites in the Fc sequence (e.g., U.S. Pat. No. 6,254,868, the Examples section of which is incorporated ...
  http://www.patentsencyclopedia.com/app/20130022541
*  ANTI-HtrA1 ANTIBODIES AND METHODS OF USE - Patent application
... light chain variable domain of an antibody. In certain embodiments, a single-domain antibody is a human single-domain antibody ... sufficient affinity such that the antibody is useful as a diagnostic and/or therapeutic agent in targeting HtrA1. In one ... the antibody. [0199] b) Glycosylation Variants [0200] In certain embodiments, an antibody provided herein is altered to ... Taqman Gene Expression Master Mix (Applied Biosystem) and Taqman Gene Expression Assay primers (Applied Biosystem) to exon 1-2 ...
  http://www.patentsencyclopedia.com/app/20130129743