Patent US5200313 - Nucleic acid hybridization assay employing detectable anti-hybrid antibodies - Google Patents
... selective for binding the hybrids in the presence of the single stranded sample and probe nucleic acids. No immobilization or ... A nucleic acid hybridization assay employing an immobilized or immobilizable polynucleotide probe selected to form DNA.RNA or ... Genomic Vision. Diagnosis of viral infections by detection of genomic and infectious viral dna by molecular combing. ... DNA probes can be used advantageously when the sample is RNA.. Immobilization of the Probe. As described previously, the probe ...http://www.google.ca/patents/US5200313
AB INITIO GENERATION OF SINGLE COPY GENOMIC PROBES - Patent application
Ab initio generation of single copy genomic probes. 2010-01-07. Single copy genomic hybridization probes and methods of ... A probe prepared from this interval would be of adequate length and suitable for use as a genomic probe (for FISH, microsphere ... genomic probes is essential to ensure that diagnostic probes specifically recognize only a single location in the genome. These ... copy probe and other genomic locations that are partially paralogous to the entire potential probe sequence or a portion ...http://www.patentsencyclopedia.com/app/20100240880
Technology to Identify and Assay Chemical Genomic Probes - Ronald Davis
The primary objective of this proposal is to develop and deploy cutting edge technologies and chemical genomic tools and to ... Technology to Identify and Assay Chemical Genomic Probes Davis, Ronald Wayne Stanford University, Stanford, CA, United States ... Technology to Identify and Assay Chemical Genomic Probes. Davis, Ronald Wayne / Stanford University. $1,117,030. ... Technology to Identify and Assay Chemical Genomic Probes. Davis, Ronald Wayne / Stanford University. $1,117,031. ...http://grantome.com/grant/NIH/R01-HG003317-06
Background plaques when screening Lambda ZAP II genomic libraries with DNA probe
... Michael icarus at uvic.ca Tue Jan 27 22:48:35 ... I'm currently have some trouble screening a ZAP II library composed of genomic DNA of my bacteria of interest. My probe is a ... I screened around 36,000 plaques (genome of origin is around 4Mbp). As a +ve control, I dot 44ng of unlabelled probe DNA on ...http://www.bio.net/bionet/mm/methods/1998-January/064182.html
Automated design of genomic Southern blot probes - Probe Design 1 - Genes2Cognition Neuroscience Research Programme
Unique Probes. Non-Unique Probes Passed. Total Probes Passed. Total Candidate Probs. Total Probes Passed (%). Candidate Probes ... Average unique probes. Average non-unique probes passed. Average total probes passed. Average per design. Average total probes ... Probe Design. Mouse Chr. Passed. Genomic Design Window (bases). Length Best Probe (bases). Best Score Ratio. ... Automated design of genomic Southern blot probes. Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant ...http://www.genes2cognition.org/software/southern_blot/
Moth sex chromatin probed by comparative genomic hybridization (CGH) : HUSCAP
Comparative genomic hybridization (CGH) with a probe mixture of differently labeled genomic DNA from females and males ... Moth sex chromatin probed by comparative genomic hybridization (CGH). フルテキスト Genome46(2).pdf. 1.16 MB. PDF. 見る/開く. ... Moth sex chromatin probed by comparative genomic hybridization (CGH). 著者: Sahara, Ken 著作を一覧する. Marec, František 著作を一覧する. ...https://eprints.lib.hokudai.ac.jp/dspace/handle/2115/891
Molecular Cytogenetics and Genome Organization - Pat Heslop-Harrison
Examples shown use two cloned probes or two genomic probes. More or only one probe may be used, and cloned and genomic probes ... 2x probe; blocking clones, 10x probe; genomic DNA probe for detection of genomes in plant hybrids, 50x. If genomic probes are ... Probe concentration is approximate *Excess amounts of unlabeled blocking DNA genomic DNA or reannealed Cot=1 DNA are used to ... genomic DNA and Section 7.3). Appropriate multiples of probe concentration to try are: CISS for human chromosomes, ...https://www.le.ac.uk/biology/phh4/prochroprep.htm
Mutations in the RNA granule component TDRD7 cause cataract and glaucoma. - PubMed - NCBI
The black bar indicates TDRD7 genomic probe in Southern analysis. TaqI and StuI refer to the fragments resulting from ... whereas green anchor probe remains intact in inv(). White arrowheads indicate inverted genomic region that hybridizes to TDRD7- ... The red bar indicates fosmid clone G248P8912G10 used as a TDRD7-specific probe. Exons are indicated by purple boxes. (C) ... specific probe; white arrow represents noninverted region (see ). (D) TDRD7 haploinsufficiency is demonstrated by Western blot ...https://www.ncbi.nlm.nih.gov/pubmed/21436445?dopt=Abstract
Probing the HIV-1 genomic RNA trafficking pathway and dimerization by genetic recombination and single virion analyses. -...
These findings provide unique insights into the process of RNA export in general, and more specifically, of HIV-1 genomic RNA ... Probing the HIV-1 genomic RNA trafficking pathway and dimerization by genetic recombination and single virion analyses. ... Probing the HIV-1 genomic RNA trafficking pathway and dimerization by genetic recombination and single virion analyses. ... These findings provide unique insights into the process of RNA export in general, and more specifically, of HIV-1 genomic RNA ...https://www.neuroscience.ox.ac.uk/publications/228616
BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut | BMC Plant Biology |...
Whole genomic DNA from A. duranensis and A. ipaënsis were used as probes in genomic in situ hybridization (GISH). Probes were ... Total genomic DNA of A. duranensis (AA genome) and A. ipaënsis (BB genome) when used as probes on the chromosomes of A. ... Genomic probes. Estimation of organelle contamination in both libraries was evaluated by hybridization of high-density filters ... Genomic in situ hybridization (GISH) of A. hypogaea metaphase chromosomes with total genomic DNA from the AA genome of A. ...https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-8-14
Genomic Analysis Using High-Density Single Nucleotide Polymorphism-Based Oligonucleotide Arrays and Multiplex Ligation...
Molecular Probes). Probes for the Ewing's sarcoma region in 22q12 were used simultaneously with the probe for INI1 as an ... 8 This kit contains 2 probes for each of the 9 exons of INI1, probes for 9 other genes on chromosome 22, and 14 control probes ... The probes were applied to slides of the tumor cells and codenatured at 75°C on an Isotemp 125D heat block (Fisher Scientific ... All genomic positions were based on National Center for Biotechnology Information Build 36 of the human genome (hg18) from the ...http://clincancerres.aacrjournals.org/content/15/6/1923
Genes | Free Full-Text | Using Multiple Phenotype Assays and Epistasis Testing to Enhance the Reliability of RNAi Screening...
1. C. elegans as A Model for Probing Genomic Control of Muscle Protein Degradation. Caenorhabditis elegans is a free-living ... Mohr, S.; Bakal, C.; Perrimon, N. Genomic screening with RNAi: Results and challenges. Annu. Rev. Biochem. 2010, 79, 37-64. [ ... We found a similarly low false negative rate in our (unpublished) screens of the full genomic sets of protein kinases (3%) and ... Fraser, A.G.; Kamath, R.S.; Zipperlen, P.; Martinez-Campos, M.; Sohrmann, M.; Ahringer, J. Functional genomic analysis of C. ...http://mdpi.com/2073-4425/3/4/686/htm
Thermogenin - Wikipedia
Detection of brown adipose tissue uncoupling protein mRNA in adult patients by a human genomic probe. . In: Clin. Sci.. . 75, ...https://de.wikipedia.org/wiki/Thermogenin
Patent US4675283 - Detection and isolation of homologous, repeated and amplified nucleic acid ... - Google Patents
DNA from one of these clones was used as a probe for hybridization with a restriction digest of genomic DNA by the procedure of ... USA 80:4069-4073 (1983)] and the cloning of amplified DNA sequences after differential screening with genomic probes [Brison et ... problem when the probe comprises a genomic clone of eukaryotic DNA for hybridization with a restriction digest of total genomic ... Specific DNA probes in diagnostic microbiology. US4395486 *. Aug 19, 1981. Jul 26, 1983. Medical College Of Ga. Research Inst ...http://www.google.com/patents/US4675283?dq=5754119
Analysis with genomic probes from the regions of the deleted exons confirmed the absence of these exons (Fig.1 b). The effect ... The diagnostic probes include a flanking probe to genotype ES cells and animals (external probe 1, Ext-1) and two internal ... Southern blot analysis using a flanking genomic probe detected a new 3.2 kb mutant fragment in the heterozygote (+/−) and ... animals using each of the three probes as indicated. The small arrow indicates the mutant band with the Ext-1 probe. Constant ...http://www.jneurosci.org/content/17/23/9165
Isolation of Specific Clones from Nonarrayed BAC Libraries through Homologous Recombination
... genomic probes  were designed in correspondence of genomic regions highly conserved between human and mouse. Such probes ... To test the feasibility of HR screening of BAC libraries using multiple probes, we prepared a BAC library from genomic DNA of ... Genomic Library Amplification. To amplify the genomic library 16,000 clones were plated at a density of 3,000 cfu on 5 Petri ... 300 electroporations with 300 HR probes.. The genomic representation of the amplified library was evaluated by PCR analysis of ...https://www.hindawi.com/journals/bmri/2011/560124/
A genomic probe for GLI (pKK36P1) was kindly provided by Dr. B. Vogelstein (Johns Hopkins University, Baltimore, MD; Ref. 1 ). ... Southern hybridization of high molecular weight DNA was performed using the radiolabeled genomic GLI probe pKK36P1. Comparison ... B. Vogelstein (Johns Hopkins University, Baltimore, MD), for providing the pKK36P1 probe and to Dr. D. D. Bigner (Duke ... Salgaller M., Pearl D., Stephens R. In situ hybridization with single-stranded RNA probes to demonstrate infrequently elevated ...http://cancerres.aacrjournals.org/content/59/8/1890
Frontiers | Genetic Pharmacotherapy as an Early CNS Drug Development Strategy: Testing Glutaminase Inhibition for Schizophrenia...
Southern blot analysis used a 32P-labeled external probe (Probe A: 336 bp genomic probe 5′ of the targeting construct; Figure ... An external probe, Probe A, identified a band of genomic DNA of 5 kb in ES cells showing homologous recombination, in contrast ... A2) In a HindIII digest of ES cell DNA, Probe A hybridized to a 6.5 kb band for the WT allele, in contrast to a 5.0 kb band for ... Genomic DNA was removed by digestion with 1 μl DNase (Promega). Reverse transcription was carried out using a High Capacity ...https://www.frontiersin.org/articles/10.3389/fnsys.2015.00165/full
A new cacao linkage map based on codominant markers: development and integration of 201 new microsatellite markers |...
... for providing us with 19 microsatellites and seven genomic probes. ... A genomic library enriched for (GA)n and (CA)n was constructed, and 201 new microsatellite loci were mapped on 135 individuals ... Genomic exploration of the hemiascomycetous yeast: 2. Data generation and processing. FEBS Lett 487:6-13CrossRefGoogle Scholar ...https://link.springer.com/article/10.1007%2Fs00122-003-1533-4
Patent US5631142 - Compositions comprising bone morphogenetic protein-2 (BMP-2) - Google Patents
All recombinants which hybridize to the bovine genomic probe which do not hybridize to the BMP-2B probe are picked and plaque ... "N" means either A, T, C or G. These probes are radioactively labeled and employed to screen a bovine genomic library. The ... Duplicate nitrocellulose replicas are made of the secondary plates and both sets hybridized to the bovine genomic probe as the ... A 32 P-labeled single-stranded DNA probe is made from a template preparation of this subclone. This probe is used to screen ...http://www.google.com/patents/US5631142?dq=5,072,412
Plant Production and Delivery System for Recombinant Proteins as Protein-Flour or Protein-Oil Compositions - University of...
Probing genomic diversity and evolution of Escherichia coli 0157 by single nucleotide polymorphisms. Genome Res 16, 757-67. ... The term "gene" encompasses both cDNA and genomic forms of a gene. A genomic form or clone of a gene contains the coding region ... In addition to containing introns, genomic forms of a gene can also include sequences located on both the 5′ and 3′ end of the ... Larvae were counted as dead if they do not move upon probing. Error bars represent the Standard Deviation among samples. ...http://www.freepatentsonline.com/y2011/0263487.html
Patent US8182995 - Real time gene expression profiling - Google Patents
Single copy genomic hybridization probes and method of generating same. WO2002016652A2. 24 Aug 2001. 28 Feb 2002. Genome ... Methods for combined PCR amplification and hybridization probing using doubly labeled fluorescent probes. ... During amplification, the probe is digested by the nuclease activity of a polymerase when hybridized to the target sequence to ... In the beacon approach, a probe is designed with a stem-loop structure, and the two ends of the molecule are labeled with a ...http://www.google.ca/patents/US8182995
Characterization of large genomic deletions in the FBN1 gene using multiplex ligation-dependent probe amplification | BMC...
Our findings expand the number of large FBN1 deletions, and emphasize the importance of screening for large genomic deletions ... and TGFβR2 genes using multiplex-ligation dependent probe amplification (MLPA) in patients with aortopathy, in whom no ... Multiplex Ligation-dependent Probe Amplification (MLPA). Genomic DNA was extracted from peripheral blood using standard ... Characterization of large genomic deletions in the FBN1 gene using multiplex ligation-dependent probe amplification. ...https://0-bmcmedgenet-biomedcentral-com.brum.beds.ac.uk/articles/10.1186/1471-2350-12-119
Search results | IslandScholar
Development of genomic probes to Sarcocystis cruzi (Apicomplexa) Source Veterinary Parasitology Date 1991 Author Kibenge, ... Use of genomic DNA probes for the diagnosis of acute sarcocystosis in experimentally infected cattle Source Veterinary ... A genomic library of Sarcocystis cruzi sporozoite DNA was constructed in bacteriophage lambda gt10. Recombinant phages ... Two clones of 1.4 and 4.33 kilobase pairs (kbp) DNA inserts, were selected from a Sarcocystis cruzi sporozoite genomic library ...http://islandscholar.ca/islandora/search/mods_identifier_u2_mt%3A%28%22Pathology%20and%20Microbiology%22%29?sort=mods_canonical_date_issued_dt%20desc&islandora_solr_search_navigation=1&f%5B0%5D=mods_relatedItem_host_titleInfo_title_s%3A%22Veterinary%5C%20Parasitology%22
"Distortion of Quantitative Genomic and Expression Hybridization by Cot" by Heather L. Newkirk, Joan H. M. Knoll et al.
When added to target DNA, Cot-1 enhanced hybridization (2.2- to 3-fold) to genomic probes containing conserved repetitive ... Hybridization measurements with such probes are less reproducible in the presence of Cot-1 than for probes derived from sc ... defined sc probes lacking repeats or by substituting synthetic repetitive elements complementary to sequences in genomic probes ... association between these sequences and probes distort quantitative measurements of the probes hybridized to desired genomic ...https://ir.lib.uwo.ca/biochempub/11/