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*  Pulsed Field Gel Electrophoresis - A. P. Monaco - Oxford University Press
DNA fragments greater than 50 kb and has made a significant impact on the analysis of both prokaryotic and eukaryotic genomes. ... PFGE analysis of yeast artificial chromosomes. 7. Functional analysis of mammalian genomes using yeast artificial chromosomes. ... Pathogens and Disease. Patrik Bavoil * Methods in Yeast Genetics and Genomics. Maitreya J. Dunham, Marc R. Gartenberg, and ... and transfer of yeast artificial chromosomes (YACs). The final two chapters describe the use of PFGE in the analysis of ...
  https://global.oup.com/academic/product/pulsed-field-gel-electrophoresis-9780199635351?cc=us&lang=en&tab=description
*  Bacterial recombination | Article about bacterial recombination by The Free Dictionary
In bacteria and the yeast S. cerevisiae, integration of such DNA into the genome requires substantial sequence similarity ... This artificial recombination depends on the use of site-specific endonucleases (restriction enzymes) and DNA ligase. See Gene ... Homologous chromosomes approach each other and with great precision line up side by side (synapsis); this is followed by the ... great diversity of gene sequences encoding the antibodies and T-cell receptors necessary for immune defense against pathogens. ...
  https://encyclopedia2.thefreedictionary.com/bacterial+recombination
*  Modification of flavonoid biosynthesis in plants - Agriculture Victoria Services Pty Ltd
... yeast artificial chromosomes; bacterial artificial chromosomes; binary bacterial artificial chromosomes; vectors derived from ... A genetic marker identifies a specific region or locus in the genome. The more genetic markers, the finer defined is the ... insects and fungal pathogens. 14. A method of modifying a flavonoid-related biological property of a plant comprising ... Causier, B. and Davies B. (2002). Analysing protein-protein interactions with the yeast two-hybrid system. Plant Mol. Biol. 50 ...
  http://www.freepatentsonline.com/9567600.html
*  Characterization of yeast artificial chromosomes from Plasmodium falciparum: construction of a stable, representative library...
... falciparum strain FCR3 in yeast as artificial chromosomes. A pYAC4 library with an average insert size of approximately 100 kb ... Unlike bacterial hosts, yeast stably maintain and propagate large tracts of parasite DNA. Long-range restriction enzyme mapping ... Since the telomeric ends of chromosomes are underrepresented in YAC libraries, we have enriched for these sequences by cloning ... Pulsed-field gel electrophoretic separation of P. falciparum chromosomes has shown that large chromosomal polymorphisms, ...
  https://www.semanticscholar.org/paper/Characterization-of-yeast-artificial-chromosomes-f-Bruin-Lanzer/941c1175c8b90e45070d862fc69524c9c64387fd
*  YAC Protocols | Springer for Research & Development
Transformation of Yeast Using Bioactive Beads With Surface-Immobilized Yeast Artificial Chromosomes ... Contribution of Yeast Artificial Chromosome-Based Physical Maps to the Final Assembly of the Trypanosoma cruzi Genome ... Construction of Yeast Artificial Chromosome Libraries From Pathogens and Nonmodel Organisms Cecilia P. Sanchez, Michael Lanzer ... Chain Reaction-Mediated Yeast Artificial Chromosome-Splitting Technology for Generating Targeted Yeast Artificial Chromosomes ...
  https://rd.springer.com/book/10.1385%2F1597451584
*  nQuire: a statistical framework for ploidy estimation using next generation sequencing | BMC Bioinformatics | Full Text
nQuire is likely to be useful in epidemiological studies of pathogens, artificial selection experiments, and for historical or ... the utility of nQuire analyzing individual samples of the pathogenic oomycete Phytophthora infestans and the Baker's yeast ... nQuire handles large genomes at high coverage efficiently and uses standard input file formats. We demonstrate ... A natural extension of analyzing the genome by chromosomes is to use sliding windows to detect possible transitions between ...
  https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-018-2128-z
*  Targeting DNA Double-Strand Breaks with TAL Effector Nucleases | Genetics
... of native or artificial TALEs suggests a variety of applications for these proteins in the targeted modification of genomes. In ... TALEN activity was measured in an in vivo yeast assay that is described in the text (Townsend et al. 2009). N, N terminus; NLS ... 2009; Moscou and Bogdanove 2009). TALEs are produced by plant pathogens in the genus Xanthomonas, which deliver the proteins to ... In the absence of a repair template, broken chromosomes are rejoined by nonhomologous end joining, which often introduces short ...
  http://www.genetics.org/content/186/2/757
*  Replacing a Genome Boosts Race to Develop Designer Bugs: Study ( Researchers transformed one bacterial s...)
Craig Venter who had a hand in mapping the human genome said a...The experiment marks the most ambitious attempt yet to re- ... Genome,Boosts,Race,to,Develop,Designer,Bugs:,Study,medicine,medical news today,latest medical news,medical newsletters,current ... Dog (Mans best friend) Genome Sequenced. 6. Decoding the Fungi Genome Will Save Millions of Lives.. 7. Yeast Genome Unravels ... Both of these bacteria, which are innocuous goat pathogens, lack an outer membrane, facilitating genome transfer. Before ...
  http://www.bio-medicine.org/medicine-news/Replacing-a-Genome-Boosts-Race-to-Develop-Designer-Bugs-3A-Study-22300-1/
*  Difference between revisions of "Vectors" - OpenWetWare
Yeast. Nomenclature and types Yeast artificial chromosomes Plant. CAMBIA Plant Transformation Vectors ... yeast, and several bacterial pathogens. All clones can be requested individually, and ORF clones can also be viewed and ... useful for a variety of techniques, and open reading frame (ORF) clones relevant to human, yeast, and several bacterial ... VectorDB contains information about many common vectors, including yeast vectors. The EMBL Hamburg outstation maintains a large ...
  https://openwetware.org/wiki/?title=Vectors&diff=273878&oldid=8016
*  Promoter From Z. Mais - Patent application
... retroviral vectors as well as artificial chromosomes, such as bacterial or yeast artificial chromosomes. Moreover, the term ... The Expression Profiles of Zm.348.2.A1_a_at Using Affymetrix GeneChip® Maize Genome Array Analysis [0137] Total RNA isolated ... various proteins for defense against pathogens, and many more, and v) bring about improved effects in the plants. [0032] The ... 1995, Yeast 11, 1233-1240; Sanz et al. (1994) Yeast 10:1195-1202, sequence: GenBank Acc. No.: NC001140 chromosome VIII, ...
  http://www.patentsencyclopedia.com/app/20110252504
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... whose members possess readable polytene chromosomes 1. As a result of genome mapping efforts, 88% of the An. gambiae genome has ... The mosquito midgut and salivary glands are key entry and exit points for pathogens such as Plasmodium parasites and Dengue ... mediated targeted mutagenesis has been used to introduce point mutations into genes of interest in cultured cells of both yeast ... provides a useful alternative for introducing artificial single nucleotide substitutions into episomal and chromosomal DNA gene ...
  https://www.jove.com/visualize/abstract/21912622/the-interplay-between-tubulins-p450-cytochromes-during-plasmodium
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... artificial chromosomes, RNA, proteins and small molecules into mice, rats and, to a limited degree, other animals. This ... elegans genes that protect against Salmonella and other intestinal pathogens using genome-wide RNAi libraries. ... Large-scale GI maps have been reported for eukaryotic organisms like yeast3-7, but GI information remains sparse for ... Since C. elegans whole genome RNAi libraries are available, this protocol makes it possible to comprehensively screen for C. ...
  https://www.jove.com/visualize/abstract/24376673/proof-concept-to-use-malignant-b-cell-line-drug-screen-strategy-for
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Immunology, Issue 83, Microdissection, whole genome amplification, malaria mosquito, polytene chromosome, mitotic chromosomes, ... and difficulties in genetically manipulating certain pathogens. Expression of type III effectors in the budding yeast ... or artificial transformation of the latter; and the excision of the plasmid is random and can either restore the initial ... organization of chromosomes in the cell nucleus. The advent of laser capture microdissection (LCM) and whole genome ...
  https://www.jove.com/visualize/abstract/22479512/identification-chromosomal-genes-yersinia-pestis-that-influence-type
*  View source - 2011.igem.org
This year, the UCSF iGEM team has engineered artificial biofilms via yeast cell surface display. We synthetically engineered S ... Getting close to pathogens, it sends the killer gene to them with conjugation. Then pathogens will be killed. To accomplish ... Further, we employed multiplex automated genome engineering (MAGE) for facile editing of the E. coli genome enabling rapid ... series of plasmids that allows the user to integrate constructs into lambdoid phage sites common to many bacterial chromosomes ...
  http://2011.igem.org/wiki/index.php?title=Jamboree/Team_Abstracts&action=edit
*  Artificial gene synthesis - Wikipedia
Special Issue SYNTHETIC YEAST GENOME Science 10 March 2017 Vol 355, Issue 6329 Fikes, Bradley J. (May 8, 2014). "Life ... In March 2017 6 yeast chromosomes were reported to have been synthesized. DNA sequences have been described which use newly ... cloned into plasmids and the ability to safely obtain genes for vaccine research without the need to grow the full pathogens. ... The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs ...
  https://en.wikipedia.org/wiki/Artificial_gene_synthesis
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... artificial chromosomes, RNA, proteins and small molecules into mice, rats and, to a limited degree, other animals. This ... To improve the efficiency of genome-wide single gene deletion, we attempt to establish a high-throughput technique for genome- ... The host immune response to pathogens is a complex biological process. The majority of in vivo studies classically employed to ... It is therefore an ideal approach for confirming putative interactions suggested by yeast two-hybrid or mass spectrometry ...
  https://www.jove.com/visualize/abstract/25229777/gene-expression-pathway-analysis-effects-cmah-deactivation-on-mouse
*  Genetics - Medical Microbiology - NCBI Bookshelf
Phage P1 cosmids can accept inserts up to 100 kbp, and still larger DNA molecules can be cloned in yeast artificial chromosomes ... The E. coli genome is only about 0.1% as large as the human genome, but it is sufficient to code for several thousand ... and synthesis by pathogens of specific virulence factors during growth in their host animals. The amount of a specific protein ... Recombination is the rearrangement of donor and recipient genomes to form new, hybrid genomes. Transposons are mobile DNA ...
  https://www.ncbi.nlm.nih.gov/books/NBK7908/?report=reader
*  Tettelin, Hervé | University of Maryland School of Medicine
... starting with eukaryotic chromosomes (yeast and malaria parasite) then shifting towards bacterial pathogens. I was the lead ... Genome analyses have transitioned from single or few genomes to hundreds to thousands of genomes. Pan-genome analyses are now ... Bacterial Artificial Chromosomes, pp. 309-318, Zhao S. and Stodolsky M. Eds, Humana Press Inc. (Totowa, NJ, USA). ... We demonstrated that for many bacteria, including many human pathogens, this intra-species diversity results in a pan-genome ...
  http://www.medschool.umaryland.edu/profiles/Tettelin-Herve/
*  Mirror-Image Cells Could Transform Science - or Kill Us All | WIRED
... the protective end caps of chromosomes. He also created the artificial yeast chromosome, critical to advances in DNA cloning ... Indeed, most of the basic molecules of life-from the nucleic acids of the genome to the amino acids of the proteins-have mirror ... "It can't infect us." Just as viruses from our side of the mirror can't infect it, mirror pathogens can't infect us. ... All of us earthlings, from algae to elephants, have proteins made of left-handed amino acids and a genome of right-handed ...
  https://www.wired.com/2010/11/ff_mirrorlife/
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Here we present the protocol for the incorporation of ribonucleotides into the genome of the eukaryotic model system yeast / ... simultaneous detection of Xist RNA and DNA encoded by the X chromosomes. This combined DNA-RNA FISH protocol can likely be ... gondii and related significant human pathogens that cause malaria (Plasmodium sp.) and cryptosporidiosis (Cryptosporidium). ... Bacterial artificial chromosome (BAC), DNA-probe, Rnf12 ... thereby allow genome-wide insights. The utilization of yeast as ...
  https://www.jove.com/visualize/abstract/21249130/gadd45a-is-an-rna-binding-protein-and-is-localized-in-nuclear-speckles
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... and difficulties in genetically manipulating certain pathogens. Expression of type III effectors in the budding yeast ... To improve the efficiency of genome-wide single gene deletion, we attempt to establish a high-throughput technique for genome- ... Briefly, the integral membrane bait protein is fused to Cub which is linked to an artificial transcription factor. Prey ... Several methods are available to manipulate bacterial chromosomes1-3. Most of these protocols rely on the insertion of ...
  https://www.jove.com/visualize/abstract/24594763/analysis-of-the-promoters-involved-in-enterocin-as-48-expression
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... whose members possess readable polytene chromosomes 1. As a result of genome mapping efforts, 88% of the An. gambiae genome has ... Explanation is given for how key refractory genes, those genes conferring resistance to vector pathogens, are identified in the ... mediated targeted mutagenesis has been used to introduce point mutations into genes of interest in cultured cells of both yeast ... provides a useful alternative for introducing artificial single nucleotide substitutions into episomal and chromosomal DNA gene ...
  https://www.jove.com/visualize/abstract/25370807/first-report-an-exophilic-anopheles-arabiensis-population-bissau-city
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... of the human genome (note that exons span 2% of our genome). Some HERV loci still express proteins that have been associated ... X-inactive specific transcript (Xist) lncRNA accumulation (called an Xist cloud) on one of the two X-chromosomes in mammalian ... It is applicable to a broad range of organisms including vertebrates, Drosophila, and yeast. We successfully applied 4sU- ... Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens ...
  https://www.jove.com/visualize/abstract/24937006/a-method-for-detecting-long-non-coding-rnas-with-tiled-rna-expression
*  Xenotransplantation - the transplant of organs or tissues between different species has become a major issue within the past
... to use whole yeast artificial chromosomes (YACs) containing large segments of the human genome to optimize gene expression, but ... This raised concerns over the creation of cross-species pathogens in xenotransplantation. ... required for reverse-transcription and integration of the viral genome into the host genome) (13). ... The use of artificial organs and human cells and tissues will both avoid the risk of cross-species viral epidemics. ...
  https://www.iatp.org/files/Xenotransplantation_How_Bad_Science_and_Big_Bu.htm
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... artificial chromosomes, RNA, proteins and small molecules into mice, rats and, to a limited degree, other animals. This ... The Δku80 strains make functional genomic studies feasible on the single gene as well as on the genome scale1-4. Here, we ... The human WRN gene and associated variants, cloned into DNA plasmids for expression in yeast, can be placed under the control ... gondii and related significant human pathogens that cause malaria (Plasmodium sp.) and cryptosporidiosis (Cryptosporidium). ...
  https://www.jove.com/visualize/abstract/24223931/identification-wee1-like-kinase-gene-essential-for-procyclic